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North Dakota State Universitywww.ndsu.edu/bioopportunities
Ethanol Production from Sugar Beet Pulp
Sugar beet plant
Impa
ct
• NorthDakotaandMinnesotaproduce15.8milliontonsofsugarbeetsperyear,whichismorethan50%oftotalU.S.production
• 750,000drytonsoflow-valuepulpremainaftersucroseextractioninNorthDakotaandMinnesota
• NorthDakotaandMinnestoacouldproduce75millionto90milliongallonsofethanolperyearifallcarbohydratesinbeetpulp(70-75%)canbeused
ScottPryor,NurunNahar,andRachelRorickNDSUDeptofAgriculturalandBiosystemsEngineering
Todeterminethetechnicalfeasibilityofsequentialenzymetreatmentstoproduceseparatefermentablesugarstreamstoincreasecelluloseloadingratesandethanoltitersfromsugarfermentation.Separatesugarstreamsalsomaybefermentedintootherhigher-valuefuelsorchemicals.O
bjec
tive
• Minimaladditionalcostsforharvest,storageortransportationofsugarbeetpulp
• Eliminationofdryingandpressingprocessesforpulp;energyfordryingisestimatedat$80/ton
• Lowlignincontent(<2%)leadstoeliminationofcostlythermochemicalpretreatmentrequiredformostotherbiomassfeedstocks
• Sugarbeetpulphasahighcarbohydratecontentconsistingmainlyofcellulose,hemicelluloseandpectin
• Cellulose,hemicelluloseandpectincomponentsarereadilyhydrolyzedbycommercialenzymes
Benefits
•Relativelysmallproductionscaleonaprocessingplantbasis
• Relativelylowlevelofcellulosecontent
•Largepectinlevelsnottypicalofmostlignocellulosicbiomass
•Hemicellulosecomponenthasdifferentcompositionthanmostbiomassfeedstocks
Challenges
Bioproduct Research Laboratory
Working with sugar beet pulp
Analyzing carbohydrates and ethanol
Glucose21%
Arabinose 21%
Galactose5%
Xylose2%
Galcturonic Acid21%
Protein11%
Ash4%
Other15%
Cellulose
HemicellulosePectin
Sugar Beet Pulp
Arabinose and Galacturonic Acid Glucose
Cellulose
Ethanol
Pectinases & Hemicellulases
Cellulases
EnzymaticHydrolysis
FermentationProcess
Escherichia coli Saccharomyces cerevisiae
Flow chart of ethanol production procedure from sugar beet pulp
Sugar beet pulp composition