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Scientific studies conducted to help advance equine health and well-being
Equine Health Studies Program
2008-2010 Equine Research Report
Dr. Peter F. HaynesDean
Dr. James E. MillerInterim Associate Dean for Research and Advanced Studies
Dr. Joseph TaboadaAssociate Dean for Student and Academic Affairs
LSU SCHOOL OF VETERINARY MEDICINE ADMINISTRATION
Dr. David F. SeniorAssociate Dean for Advancement and Strategic Initiatives
Ernie TanoosAssistant Dean for Finance and Administrative Services
LETTER FROM OUR DEAN
The Louisiana State University School of Veterinary Medicine is pleased to once again present the Equine Health Studies Program’s Equine Research Report, which covers scientific activities of the program from 2008 through 2010. Central to the program’s mission is the health, well-being and performance of horses supported through state-of-the-art research that benefits the horse-owning public in Louisiana and beyond. As a former equine surgeon and faculty member, I have watched the EHSP grow and flourish, as evidenced by contents of this Research Report, translating research into practical solutions for our broad-base constituents and clients. In addition to its research prowess, the program’s dedicated faculty and staff provide clinical service, education, and community outreach.
The EHSP has made significant advances in research collaborations with industry to extend its work in the areas of laminitis prevention; lameness, orthopedics and biomechanics; reproductive disorders; respiratory and gastrointestinal diseases including the treatment and prevention of gastric ulcer disease; equine Cushing’s disease; and surgery that will impact equine veterinary care for years to come.
The EHSP continues to build and maintain strong relationships and community engagement with the stakeholders of Louisiana so that it can be responsive to the needs of horses in the region. In the aftermath of Hurricanes Gustav and Ike and the Gulf Oil Spill, the SVM was able to step in and help with the rescue and care of animals and wildlife in south Louisiana. The EHSP, in concert with the SVM and the Louisiana State Animal Rescue Team, has been on the forefront of emergency preparedness, and continues to be a model for other veterinary institutions. Additionally, the EHSP continues to provide strong leadership within the State and has been actively involved with referring veterinarians, the Louisiana Racing Commission, the Louisiana Horse Council, the Louisiana Thoroughbred Breeders Association, and many other organizations to provide the horsemen with the latest information and quality service.
Through the dedicated efforts of its faculty and staff, and the continued facility and equipment enhancements in support of its activities, this multidisciplinary program is a real “Point of Pride,” reflects a very positive trajectory, and continues to bring national and international recognition to the School.
Sincerely,
Peter F. Haynes, DVM, DACVSDean
Dr. Frank AndrewsDirector of the Equine Health Studies Program
Ky MortensenDirector of Advancement and Client Services
Dr. Margaret CoffeyDirector of the LSU Veterinary Teaching Hospital
Ginger GuttnerDirector of Public Relations
The Louisiana State University School of Veterinary Medicine continues to promote a high level of support for equine health through teaching, research and service. The equine industry is an integral part of the Louisiana livestock community, especially the racing industry. Our Equine Health Studies Program is the basis for the research effort that investigates and provides up to date and pertinent information for teaching students and extending service to the public. It is important for our faculty to continue building through extra-mural support and we have been very fortunate to have a number of sources that include federal (NIH, USDA), state (Equine health appropriation) and private/industry (Grayson, Elanco, Boehringer Ingelheim Vetmedica, Zinpro Corporation, Centaur Corporation, Seabuck Equine, LLC, SmartPill Corporation, Pall Corporation) funding. The faculty contributing to the program are to be commended for their concerted efforts to ensure that equine health remains as one of the top priorities of the School.
Sincerely,
James E. Miller, DVM, MPVM, PhDInterim Associate Dean for Research & Advanced StudiesProfessor of Epidemiology & Community Health
LETTER FROM OUR INTERIM ASSOCIATE DEAN FOR RESEARCH & ADVANCED STUDIES
LETTER FROM OUR DIRECTOR
It’s my pleasure to introduce the 2008-10 Equine Research Report from the Equine Health Studies Program (EHSP), Louisiana State University (LSU) School of Veterinary Medicine (SVM)! As director, I am honored to present this research report documenting the productive faculty here at LSU.
The EHSP team continues to work toward the missions of clinical service, teaching, and research. As you can see in the contents of this report, faculty and staff continue to provide high quality biomedical research to address the health needs of the horse industry in Louisiana, the U.S., and the world. I congratulate each and everyone in the program for their continued commitment and devotion to excellence of the EHSP!
As you read this report, the EHSP biomedical research team at the LSU SVM has diverse research interests with one major goal–to improve the health and welfare of the horse. Investigators represented in this report contributed important information in areas of Emergency Response, Gastrointestinal Disease, Laminitis, Musculoskeletal Injury and Lameness, Orthopedics, Reproduction, Respiratory Physiology and Disease, and Colic Surgery. Each study in this report highlights benefits to the horse industry in Louisiana and surrounding regions. Many of these research findings were presented at national and international meetings, including the American Association of Equine Practitioners, American College of Veterinary Surgery, American College of Veterinary Internal Medicine, British Equine Veterinary Association, World Congress on Disaster and Emergency Medicine, Veterinary Orthopedic Society, The Laminitis Society, Applied Equine Nutrition and Training Conference, South African Equine Veterinary Association, Association Argentina de Veterinaria Equina, and the American Society of Animal Science. As you can see, researchers in the EHSP are sought after speakers at many major conferences throughout the world.
The biomedical research outlined in this report and state-of-the-art facilities on campus are a direct result of support from the Louisiana Governor’s Biotechnology Initiative Grants Program, Louisiana Board of Regents Enhancement Grants Program, and recurrent funding through the State Legislature resulting from Louisiana racetrack slot machine
The Equine Research Report is published by the Louisiana State University, School of Veterinary Medicine, Baton Rouge, LA 70803.
Phone: (225) 578-9500
Fax: (225) 578-9605
revenue. Also, funding for many of these important projects was provided from extramural sources, including the Grayson Jockey Club Research Foundation, the Morris Animal Foundation, Merck-Merial Veterinary Student Summer Research Program, Boehringer-Ingelheim Vetmedica, Pennington Biomedical Research Foundation, Applied Biosystems, Howard Hughes Medical Institute, National Wetlands Research Center, and National Institutes of Health, among others.
Furthermore, we owe our deepest gratitude to the horses that participated in these investigational studies. The research findings presented in this report would not have been possible without the availability and use of horses. All biomedical research on animals at LSU is conducted under Federal Guidelines for the Humane Care and Use of Animals and approved by the Institutional Animal Care and Use Committee (IACUC) within the SVM. Horses were carefully and compassionately used for the advancement of equine health to discover more effective methods to diagnose, treat, and prevent illness and injuries. These horses are valued members of our program and are treated with kindness and dignity. In some cases, data obtained from medical records may have been used to identify specific risk factors and treatment outcomes for important diseases or injuries. All client and animal identity was kept in strict confidence and will not be released under any circumstances.
The faculty and staff of the EHSP are proud to present this research report and look forward to the continued participation in the biomedical research program as it moves forward. As part of the EHSP team, I am committed to furthering the health, well-being and performance of horses to the benefit of the people and horses of Louisiana. Also, with continued support from the horse industry and the State of Louisiana, we can further promote and establish the EHSP at the LSU SVM as the premier equine biomedical program in the world.
Sincerely,
Frank M. Andrews, DVM, MS, DACVIM
Professor and Director
LSU School of Veterinary Medicine on Facebook: http://www.facebook.com/LSU SVM
LSU EHSP on Facebook: http://www.facebook.com/LSUequine
LSU School of Veterinary Medicine on Twitter: http://www.twitter.com/LSUVetMed
http://www.vetmed.lsu.edu
Our Mission: The LSU Equine Health Studies Program will become a premier equine biomedical center in the 21st century through leading-edge research of equine diseases, contemporary instruction of professional veterinary students and veterinarians in advanced studies programs, and enhanced continuing education of the horse-owning public and private equine practitioners, with the ultimate goal of providing state-of-the-art diagnostic and therapeutic capabilities for critically ill and injured horses, and optimal clinical service to horsemen in Louisiana and the surrounding region.
TABLE OF CONTENTS
2008-2010 EHSP FACULTY ............................................................................................................ 4
2008-2010 PUBLICATIONS AND ABSTRACTS .......................................................................... 12
2008-2010 GRANTS ...................................................................................................................... 30
EHSP FACILITIES AND EQUIPMENT .......................................................................................... 36
EHSP DEVELOPMENT ................................................................................................................. 46
RESEARCH NEWS & DEVELOPMENT ........................................................................................ 48
BIOMECHANICS AND ORTHOPEDICS ....................................................................................... 50
Biomechanical algorithm to quantify forces from equine motion at the saddle position ...... 50
An in vitro biomechanical comparison between dynamic compression plates having a roughened contact surface and dynamic compression plates having a polished contact surface for the fixation of osteotomized equine third metacarpal bones ............................. 52
An In Vitro Cyclic Fatigue Comparison of a Modified 5.5 mm Locking Compression Plate Fixation with a 5.5 mm Locking Compression Plate Fixation of Osteotomized Equine Third Metacarpal Bones ................................................................................................................ 53
STEM CELL AND REGENERATIVE MEDICINE .......................................................................... 54
In Vitro Tissue Generation by Adult Equine Multipotent Stromal Cells on Collagen Scaffolds 54
The Equine Health Studies Program is supported with funds provided by the Louisiana State University School of Veterinary Medicine, the State of Louisiana, and contributions from private donors.
http://www.equine.vetmed.lsu.edu
TABLE OF CONTENTS
LAMINITIS ...................................................................................................................................... 56
Isolation and characterization of equine laminar progenitor cells ........................................ 56
Treatment of Experimental Equine Laminitis with Doxycycline .......................................... 58
GASTROINTESTINAL ................................................................................................................... 59
Pharmacokinetics and clinical safety of oral paste firocoxib, a COX-1 sparing NSAID, in foals ..................................................................................................................................... 59
Right Colonic Pathophysiology in Horses Administered Phenylbutazone ........................... 59
New Approaches in Treating Equine Gastric Ulcer Syndrome ............................................ 60
DISASTER RESPONSE ................................................................................................................ 62
Experiential learning in disaster response for veterinary students and veterinarians .......... 62
SURGERY ...................................................................................................................................... 63
LARMoF’s Procedure for Treatment of Cribbing in Horses: Is it working? .......................... 63
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2008-2010 EHSP FACULTY AND STAFF
Frank M. Andrews, Professor and Director Equine Health Studies Program, Veterinary Clinical Sciences
Dr. Andrews received his DVM and MS in Veterinary Medicine from Washington State University in 1983. He completed 1 year in
private practice at Associated Veterinary Clinics, Inc. in Walla Walla, Washington and completed an internal medicine residency in
1988 at The Ohio State University, College of Veterinary Medicine. Dr. Andrews became board certified in Large Animal Internal
Medicine in 1989 and was on the faculty at the University of Tennessee, College of Veterinary Medicine from 1988-2008. Dr.
Andrews is currently the LVMA Equine Committee Professor and Director of the Equine Health Studies Program. Dr. Andrews’
research focuses on investigating treatment and prevention of gastric ulcer disease, gastric emptying and gastrointestinal motility,
and treatment of pituitary par intermedia dysfunction (Equine Cushing’s Disease).
Steven A. Barker, Professor and Director Equine Medication Surveillance Laboratory, Comparative Biomedical Sciences
Dr. Barker is a professor of veterinary physiology, pharmacology and toxicology at the LSU School of Veterinary Medicine in the
Department of Comparative Biomedical Sciences. He received his BS in 1971, his MS in 1973, and his PhD in 1978, all from the
University of Alabama in Birmingham. Dr. Barker is also the director of the Analytical Systems Laboratory and the director of the
Equine Medication Surveillance Laboratory. He has served as the State Chemist to the Louisiana State Racing Commission since
1987.
Ralph E. Beadle, Professor Emeritus, Equine Medicine, Veterinary Clinical Sciences
Dr. Beadle was born and raised in Montana. He completed his pre-veterinary and veterinary education at Colorado State
University, where he was awarded a DVM in 1967. He spent the next five years at the University of Georgia, where he worked in
the Equine Clinic and obtained a PhD in Veterinary Physiology. After a period of two years spent at Michigan State University, he
has been at LSU for the rest of his professional career. During the first seven years at LSU, he was in the Department of Veterinary
Physiology, Pharmacology and Toxicology, where he taught both physiology and pharmacology. From that time until September
1999, he was in the Department of Veterinary Clinical Sciences, where he worked in the Medicine Section of the Equine Clinic. He
retired in September 1999, but since that time has continued to be involved in the activities of the Department of Veterinary Clinical
Sciences as a professor emeritus. His research interests involve non-sweating horses, horses with recurrent obstructive airway
disease, and horses affected with acute and chronic laminitis.
Hermann H. Bragulla, Assistant Professor, Comparative Biomedical Sciences
Dr. Bragulla received his DVM (1984) and PhD (1986) in Veterinary Medicine from the Ludwig-Maximilians-University in Munich/
Germany. He became a certified specialist for veterinary anatomy (1991) and received his habilitation (1996) from the Free
University Berlin in Berlin/Germany. Dr. Bragulla was on the faculty of the Department of Veterinary Anatomy, School of Veterinary
Medicine, Free University Berlin, from 1988 – 2004. He started as an instructor (1988 – 1996) and was promoted to assistant
professor (1996 – 2004). Dr. Bragulla’s research focuses on the comparative and developmental morphology of digital end organs
such as the equine hoof concerning the age-related normal structure versus disease-related changes in the structure of keratinized
tissues and changes in the papillary body and blood supply of connective tissues of digital end organs.
Daniel J. Burba, Professor Equine Surgery, Veterinary Clinical Sciences
Daniel J Burba was born in west central Pennsylvania. He completed an undergraduate study in Biology at Morehead State
University, Morehead, KY and attended the College of Veterinary Medicine at Auburn University. Dr Burba was accepted into an
internship program in Large Animal Medicine and Surgery at Oklahoma State University and subsequently completed a residency
in Equine Surgery in 1990. Dr Burba became a Diplomate of the American College of Veterinary Surgeons in 1995. He has
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2008-2010 EHSP FACULTY AND STAFF
received such rewards as the Carl J. Norden distinguished teaching award, teaching merit honor roll of Gamma Sigma Delta Honor
Society of Agriculture. Dr. Burba has published numerous book chapters on equine wound management, as well as several journal
articles and abstracts. He is a member of Phi Zeta Veterinary Medicine Honor Society, Gamma Sigma Delta Agriculture Honor
Society, Veterinary Wound Management Society, American Association of Equine Practitioners, American Veterinary Medical
Association, and American Association of Veterinary Clinicians. Dr Burba’s main interest is in orthopedics and laser cribbing
surgeries.
Renee T. Carter, Assistant Professor Ophthalmology, Veterinary Clinical Sciences
Dr. Carter received her DVM in Veterinary Medicine from Louisiana State University in 2000 and completed a rotating internship
in small animal medicine and surgery at LSU in 2001. She completed a four-year comparative ophthalmology residency and
fellowship at the University of Madison-Wisconsin in 2006 and returned to LSU as an assistant professor in Veterinary Clinical
Sciences (VCS). Dr. Carter became board certified in Veterinary Ophthalmology in 2007 and her research focuses on corneal
wound healing disorders and the role of Leptospirosis in the pathogenesis of equine recurrent uveitis.
Ann Chapman, Assistant Professor Equine Medicine, Veterinary Clinical Sciences
Dr. Chapman was born and raised in Harrisburg, Penn. She received her BA in Biology from Gettysburg College in 1990 and
worked in laboratory research for both private and government companies. She received her DVM from the LSU School of
Veterinary Medicine in 2001. After working in private practice for one year, she began her combination Equine Internal Medicine
Residency/Graduate Program at LSU in 2002. Dr. Chapman completed her residency in 2005 and became a Diplomate of the
American College of Veterinary Internal Medicine (Large Animal). She received a Masters of Science in Veterinary Clinical
Sciences in December 2006. She performs Mobile Equine Medicine Consultation throughout the state of Louisiana and Equine
Ambulatory Medicine to the local Baton Rouge community.
Shafiqul I. Chowdhury, Professor Molecular Virology, Pathobiological Sciences
Dr. Shafiqul Chowdhury received his DVM (1978) and MS (1980) in Veterinary Medicine from Bangladesh Agricultural in University
in 1978. He received his PhD in 1987 from Free University of Berlin, Germany. Dr. Chowdhury became Full Professor in the
College of Veterinary Medicine, Kansas State University in 2002. He joined LSU School of veterinary medicine in 2008. Dr.
Chowdhury’s research focuses on molecular pathogenesis of equine herpes type 1 (EHV-1) neuropathogenesis and development
of an efficacious vaccine against EHV-1. His EHV-1 research is funded by a corporate grant.
Anderson daCunha, Assistant Professor Anesthesiology, Veterinary Clinical Sciences
Dr. da Cunha is an assistant professor of veterinary anesthesiology in the Department of Veterinary Clinical Sciences. He is also
service chief of Anesthesia Services in the Veterinary Teaching Hospital & Clinics. Dr. da Cunha received his DVM from Federal
University of Parana in 2000, his MS from Federal University of Santa Maria in 2002 and after his residency at North Caroline State
University (2006) he became a diplomate of the American College of Veterinary anesthesiologists in 2009.
Fabio Del Piero, Professor of Pathology, Pathobiological Sciences
Dr. Del Piero started his career in pathology in the late eighties and worked at University of Milano, Animal Health Trust UK, Cornell
University, and University of Pennsylvania. He joined LSU in 2012 and he is currently Professor of Pathology in the Department of
Pathobiological Sciences. Dr. Del Piero investigations focus on comparative pathology with emphasis on equine pathology, and
infectious diseases.
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Susan C. Eades, Professor Equine Medicine, Veterinary Clinical Sciences
Dr. Eades graduated from the LSU School of Veterinary Medicine in 1982, then completed an internship in large animal medicine
and surgery and a residency in large animal internal medicine at the University of Pennsylvania’s New Bolton Center. She then
completed a PhD in Veterinary Physiology at the University of Georgia. Her doctoral studies concentrated on intestinal vascular
and nonvascular smooth muscle physiology and pharmacology. Upon completion of her PhD, Dr. Eades began as an assistant
professor of large animal medicine at the University of Georgia College of Veterinary Medicine, where she remained through 1997.
She returned to LSU in 1998 as an associate professor of equine medicine. Dr. Eades’ clinical interests include equine internal
medicine; however, she has a special interest in cardiology and ultrasound. Her research interests include intestinal disease and
laminitis.
Bruce E. Eilts, Professor Theriogenology, Veterinary Clinical Sciences
Dr. Eilts is originally from the Minneapolis/St. Paul area in Minnesota. He graduated from high school in West St. Paul, Minn.,
and then attended the University of Minnesota as a pre-veterinary medicine student. He obtained a BS in veterinary science
in 1975 and his DVM in 1977, both from the University of Minnesota. He was in private practice for one year before returning
to the University of Minnesota to obtain an MS in theriogenology in 1982. After two and a half years in a private practice in
southern California, he came to LSU as an assistant professor in 1984. He became board certified in the American College of
Theriogenologists in 1986. His main clinical interest and research is basic reproduction management in the horse.
Joseph Francis, Associate Professor, Comparative Biological Sciences
Dr. Francis is an associate professor in the Department of Comparative Biomedical Sciences. He received his BVSc and his MVSc
in 1990 and 1994, respectively, both from Madras Veterinary College (India). In 1999, Dr. Francis received his PhD from Kansas
State University. His research focuses on the brain mechanisms regulating cardiovascular function, specifically the understanding
of the central nervous system interactions of the cytokine renin-angiotensin-aldosterone system in heart failure.
Lorrie Gaschen, Associate Professor Radiology, Veterinary Clinical Sciences
Dr. Gaschen received her BS and DVM degrees from the University of Florida in 1985 and 1990, respectively. She received
her PhD from the University of Utrecht in the Netherlands in 2001. She is a Diplomate of Diagnostic Imaging and a European
Specialist in Veterinary Diagnostic Imaging. Dr. Gaschen is a Professor and service chief of Diagnostic Imaging in the LSU School
of Veterinary Medicine’s Veterinary Teaching Hospital & Clinics. She joined the faculty at LSU in January 2006. Dr. Gaschen’s
research interests are in vascular imaging and ultrasound of the gastrointestinal tract and pancreas and MRI.
Marjorie S. Gill, Professor Farm Animal Services, Veterinary Clinical Medicine
Dr. Gill received her DVM and MS degrees from Iowa State University in 1976 and 1984, respectively. She is a Diplomate of the
American Board of Veterinary Practitioners (Food Animal Practice). She joined the faculty at LSU in July 1984. Dr. Gill’s clinical
interests are in farm animal medicine, small ruminant medicine and surgery, animal behavior, and urogenital, gastrointestinal and
ophthalmological surgery.
Jeff Gimble, Professor, Pennington Biomedical Research Center
Dr. Gimble is a professor at the Pennington Biomedical Research Center and an adjunct professor in the Department of Veterinary
Clinical Sciences. He received his BA from Dartmouth College in 1976. He received his MA, PhD, and MD from Yale University in
1980, 1981, and 1982, respectively. Dr. Gimble collaborates on numerous adult stem cell projects within the EHSP.
2008-2010 EHSP FACULTY AND STAFF
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Samithamby Jeyaseelan, Associate Professor and Director of Lung Biology Laboratory, Pathobiological Sciences
Dr. Jeyaseelan received his DVM degree in Veterinary Medicine from the University of Peradeniya, Sri Lanka in 1992. He
completed his PhD degree in Pulmonary Immunology at the College of Veterinary Medicine, University of Minnesota in 2001. He
completed his first postdoctoral training at Yale University in Pulmonary Immunology from 2001-2003 and second postdoctoral
training at National Jewish Health/Colorado Health Sciences Center in lung Biology from 2003-2004. Dr. Jeyaseelan was on the
faculty at National Jewish Health/Colorado Health Sciences Center from 2004-2007. Dr. Jeyaseelan is currently an Associate
Professor of Immunology in Pathobiological Sciences and Director of the Lung Biology Laboratory. Dr. Jeyaseelan’s research
focuses on investigating the mechanisms underlying lung inflammation and host defense in response to bacterial pathogens. Dr.
Jeyaseelan has published more than 40 original articles and more than 12 review articles. He serves as an Associate Editor of the
Journal of Immunology, Academic editor of PLoS One and editorial boards of Clinical and Vaccine Immunology and Infection and
Immunity. Dr. Jeyaseelan’s research program is funded by the National Institutes of Health and Flight Attendant Medical Research
Institute.
Jill R. Johnson, Professor Emeritus Equine Medicine, Veterinary Clinical Sciences
Dr. Johnson is a native of South Dakota. She graduated from veterinary school at the University of Minnesota, then stayed on and
completed her MS degree in Veterinary Surgery and Radiology. She joined the faculty of the LSU School of Veterinary Medicine
in 1977. She is a specialist in internal medicine (Diplomate, American College of Veterinary Internal Medicine) and equine
practice (Diplomate, American Board of Veterinary Practitioners). Past research activities have centered on immunogenetics and
immunology, methods of quantifying exercise training using the global positioning system (GPS) and development of tissue culture
models to study diseases. Current research involves the spatial-temporal study of hospital-acquired infections in the large animal
hospital.
Michael T. Kearney, Biostatistician and Instructor, Pathobiological Sciences
Michael received his MS and MApStat degrees from LSU in 1980 and 1981, respectively. He manages statistical consulting for the
School of Veterinary Medicine with primary emphasis on linear models, repeated measures analyses, and nonparametric statistics.
He teaches graduate courses in Biostatistics and using SAS to analyze research data. He also offers a professional curriculum
elective for veterinary statistics.
Konstantin G. Kousoulas, Professor and Director BIOMMED, Pathobiological Sciences
Dr. Kousoulas is a professor of veterinary virology in the department of Pathobiological Sciences at the School of Veterinary
Medicine. He is also a professor of veterinary science in the LSU Agricultural Experiment Station, an adjunct professor of biological
sciences in the College of Basic Sciences and an adjunct professor in the Department of Microbiology and Immunology at the
LSU Health Sciences Center in New Orleans. Dr. Kousoulas is the director of the LSU School of Veterinary Medicine’s Division
of Biotechnology & Molecular Medicine. He received his BS in 1975 from Fairleigh Dickinson. In 1977, he received his MS and in
1981, he received his PhD, both from Pennsylvania State University. Dr. Kousoulas’ research interests are in molecular biology
and pathogenesis of herpes and corona viruses; experimental approaches in his laboratory involve advanced molecular biology,
molecular genetics and cell biology to study viral attachment, viral penetration into cells, cellular receptors and virus spread. Other
interests include the development of vaccines to combat animal and human viral infections, the application of viral vectors for gene
therapy, and the utilization of computers for biological research and teaching.
2008-2010 EHSP FACULTY AND STAFF
8
Jose A. Len, Theriogenology Instructor, Veterinary Clinical Sciences
Dr. Len received his DVM in veterinary medicine from the Universidad de Guadalajara, Mexico in 1994. He then worked as the
Panamanian Racing Commission Veterinarian at the Hipodromo Presidente Remon Racetrack in Panama City, Rep. of Panama
until 1999. From 1999 to 2005, Dr. Len was the veterinarian and manager of Haras Cerro Punta, a thoroughbred breeding farm in
the Republic of Panama. Dr. Len completed a theriogenology residency in 2008 at Louisiana State University, School of Veterinary
Medicine and became board certified in Theriogenology in 2009. Currently he is an instructor in Theriogenology and PhD candidate
at Louisiana State University, School of Veterinary medicine. Dr. Len’s research is focused in the improvement of processes for
semen chilling and freezing and efficient use of spermatozoa for intracytoplasmic sperm injection (ICSI) in the horse.
Mandi J. Lopez, Associate Professor and Director, Laboratory for Equine and Comparative Orthopedic Research, Veterinary Clinical Sciences
Dr. Lopez is from the Pacific Northwest. She received her bachelor’s degree from Humboldt State University in Arcata, California
and attended veterinary school at the University of California, Davis. She completed a food animal internship at Kansas State
University prior to going to the University of Wisconsin, where she completed a residency in large animal surgery and obtained
MS and PhD degrees. She then did a post-doctoral fellowship in applied biomechanics. Her areas of interest are comparative
orthopedic research and surgery. Dr. Lopez is board certified by the American College of Veterinary Surgeons. Dr. Lopez has
several patents for biomedical devices and has expertise in both applied and basic research. She came to LSU in January 2004
and directs the Laboratory of Equine and Comparative Orthopedic Research (LECOR).
Sara K. Lyle, Assistant Professor Theriogenology, Veterinary Clinical Sciences
Dr. Lyle was born and raised in Gainesville, Fla. She obtained her BS in Chemistry at Duke University and her DVM from the
University of Florida. She completed a residency in theriogenology in 1990 and her MS in reproduction in 1991 at the University
of Florida. She is board certified by the American College of Theriogenologists. She joined LSU in 2002 and completed a PhD in
2008, and is currently an Assistant Professor. Her clinical interests include mare infertility and assisted reproductive technologies.
Her research interests include reproductive immunology (equine) and assisted reproductive technologies in horses.
Charles T. “Chuck” McCauley, Assistant Professor Equine Surgery, Veterinary Clinical Sciences
Dr. McCauley joined the equine faculty in the Department of Veterinary Clinical Sciences in February 2006. Prior to joining
the faculty at LSU, Dr. McCauley was employed in a busy private referral practice in northeast Texas. He completed his BS
in Microbiology and his DVM at Texas A&M University. He successfully completed an internship and residency in food animal
medicine and surgery at Oklahoma State University. In addition, Dr. McCauley completed a residency in large animal surgery
(equine emphasis) at Purdue University. He is double boarded by the American Board of Veterinary Practitioners (Food Animal
Practice) and the American College of Veterinary Surgeons (Large Animal Surgery).
Rebecca S. McConnico, Associate Professor Equine Medicine, Veterinary Clinical Sciences
Dr. McConnico is originally from north central Ohio, where she lived for 18 years. She obtained her BS in Animal Science from
the University of Arkansas, her DVM from Louisiana State University, and her PhD and clinical residency in large animal internal
medicine from North Carolina State University. She is board certified in Large Animal Internal Medicine and her clinical interests
include equine critical care and internal medicine. She is also a certified veterinary acupuncturist. The long term goals of Dr.
McConnico’s research collaborations are elucidating the pathophysiologic mechanisms associated with intestinal diseases in
horses and determining the link between these diseases and other related abnormalities (eg., laminitis, endotoxemia, myositis),
with the broader intention of preventing, attenuating, and determining effective treatment modalities for these life-threatening
conditions. Additionally, Dr. McConnico is integrally involved in the development of the LSU-School of Veterinary Medicine’s
2008-2010 EHSP FACULTY AND STAFF
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Disaster program, a training and response program based on community involvement which hinges on growing partnerships with
other LSU units, non-government groups, and state and federal government entities.
Mustajab Mirza, Assistant Professor Equine Surgery and Emergency Service, Veterinary Clinical Sciences
Dr. Mirza received his DVM from the College of Veterinary Sciences Lahore affiliated with the University of Agriculture, Faisalabad,
Pakistan in 1992. He received his MS degree from LSU in 1998. Dr. Mirza’s primary interests are repair of long bone fractures
and pathogenesis of colics in equids, Laminitis, ophthalmology and advanced wound healing. He primarily provides after-hours
emergency services for the LSU Equine Clinic. Dr. Mirza’s research interests are in long bone fractures, performance limitations,
advanced therapeutics for osteoarthritis, and gastrointestinal disease in the horse.
Colin F. Mitchell, Associate Professor Equine Surgery, Veterinary Clinical Sciences
Dr. Mitchell, originally from Perth, Scotland, received his veterinary medical degree from the University of Edinburgh. He then
completed an internship at the University of Prince Edward Island prior to entering a combined three-year equine surgery residency
and MS graduate program at the University of Minnesota, which he completed in June 2004. He then remained on the hospital
staff at the University of Minnesota, where he worked as the equine emergency clinician/surgeon until July 2005, when he joined
the LSU School of Veterinary Medicine. He is board certified by the American College of Veterinary Surgeons. His clinical interests
include orthopedic, laparoscopic and respiratory surgery. His research interests include orthopedic implants and laminitis.
Dale L. Paccamonti, Professor and Head, Veterinary Clinical Sciences
Dr. Paccamonti, originally from Kankakee, Ill., completed his undergraduate and veterinary education at Michigan State University,
receiving his DVM in 1981. After four years in a mixed practice in Chestertown, Md., he pursued advanced training at the University
of Florida, where he completed a residency in theriogenology and received his MS degree in 1988. Dr. Paccamonti is a Diplomate
in the American College of Theriogenologists. He joined the faculty at the LSU School of Veterinary Medicine in 1988, where he is
currently head of the Department of Veterinary Clinical Sciences and a full professor of theriogenology. Dr. Paccamonti’s primary
research interests include the study of infertility in mares, assisted reproduction techniques in horses, factors affecting sperm
motility in stallions, semen cryopreservation in stallions, and the process of fetal maturation and parturition in mares. He also
collaborates in reproductive research in other domestic species. He shares responsibility for clinical theriogenology cases in all
species presented to the Veterinary Teaching Hospital & Clinics.
Daniel B. Paulsen, Professor and Director LADDL, Pathobiological Sciences
Dr. Paulsen received his BS in 1975, his DVM in 1977, and his MS in 1978, all from Kansas State University. In 1989, he received
his PhD from Oklahoma State University. Dr. Paulsen’s major research interests are bovine respiratory disease with emphasis on
Mannheimia haemolytica, Pasteurella multocida, Infectious bovine rhinotracheitis, bovine virus diarrhea, and bovine respiratory
coronavirus; pathogenesis, bacterial genetics, respiratory immunity and vaccinology; toxicologic pathology associated with inhaled
toxins and effects of inhaled substances on the pathogenesis of asthma; and application of immunohistochemical techniques in
equine respiratory disease and laminitis.
Kenneth Pierce, Assistant Professor Ophthalmology, Veterinary Clinical Sciences
Dr. Pierce, a native New Orleanian, received his Bachelor of Science from Tuskegee University in 2001 and his Doctorate of
Veterinary Medicine from Louisiana State University School of Veterinary Medicine in 2005. In 2006 Dr. Pierce completed a Small
Animal Medicine and Surgery internship at the University of Tennessee College of Veterinary Medicine. In 2007 Dr. Pierce traveled
to southern California to complete a specialty ophthalmology internship with Eye Care for Animals and B. Braun, Inc. He then
2008-2010 EHSP FACULTY AND STAFF
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completed a four year Comparative Ophthalmology residency at Michigan State University and Masters degree in 2011. Dr. Pierce
became a Diplomate of the American College of Veterinary Ophthalmologists in August 2011.
Cherie Pucheu-Haston, Assistant Professor Dermatology, Veterinary Clinical Sciences
Dr. Cherie Pucheu-Haston received her DVM from Louisiana State University in 1992, and completed an internship in Small
Animal Medicine and Surgery at LSU in 1993. She received her residency training in veterinary dermatology at North Carolina
State University in 1993-1995, and achieved board certification in Veterinary Dermatology in 1995. She worked as a specialist
in private practice for seven years, then returned to NCSU in 2002 to pursue advanced graduate training. She received her PhD
in Immunology (minor Biotechnology) from NCSU in 2006. She completed three years as a postdoctoral research associate in
the Immunotoxicology Branch of the United States Environmental Protection Agency, as a grantee from the University of North
Carolina-Chapel Hill Curriculum in Toxicology. Dr. Pucheu-Haston returned to LSU in 2011 as an Assistant Professor in Veterinary
Dermatology. Her research interests include identification of factors involved in the development and perpetuation of atopic
dermatitis, the relationships between allergic skin diseases and hypersensitivity in other organ systems (asthma, gastrointestinal
disease) and the identification of genomic or proteomic biomarkers of allergic sensitization.
Nathalie Rademacher. Dr.med.vet., Assistant Professor Radiology
Dr. Rademacher received her DVM from the Justus-Liebig-University in Giessen, Germany in 2000 and her Dr.med.vet. from the
Vetsuisse Faculty in Bern, Switzerland. After completion of 1 year small animal internship in private referral center in Switzerland,
she completed a diagnostic imaging residency in 2006 at the Vetsuisse Faculty, Zurich, Switzerland. Dr. Rademacher became
board-certified in Diagnostic Imaging with the European College of Veterinary Diagnostic Imaging (ECVDI) in 2007 and with the
American College of Veterinary Radiology (ACVR) in 2010. Since 2007, she is employed as an Assistant Professor in Radiology,
tenure track at LSU. Dr. Rademacher’s research focus is ultrasound in small and large animals, contrast enhanced ultrasound of
the pancreas in dogs and cats, elastographic ultrasound application and lung ultrasound in dogs.
Laura M. Riggs, Assistant Professor Equine Surgery, Veterinary Clinical Sciences
Dr. Riggs is originally from Memphis, Tennessee and received a B.S. in biology from Saint Louis University. She received her DVM
from the University of Tennessee College of Veterinary Medicine in 2001. She completed a 1 year large animal internship followed
by a large animal surgery residency at the University of Georgia. In 2007 she received her PhD in veterinary physiology from the
University of Georgia with research studying biomarkers in equine laminitis. Dr. Riggs is board certified by the American College of
Veterinary Surgeons. Dr. Riggs’ research focuses on lameness, laminitis and fracture repair biomechanics.
Gary A. Sod, Assistant Professor Farm Animal Services, Veterinary Clinical Sciences
Dr. Sod received his MA in Mathematics and PhD in Applied Mathematics from the University of California at Berkeley. The next 12
years were spent doing research in mathematical and computational physics, resulting in the writing of a monograph on numerical
methods in fluid dynamics and 42 journal publications. Dr. Sod served as a professor in the Department of Mathematics and an
adjunct professor in the Department of Mechanical Engineering and at Tulane University from 1985 through 1997. Dr. Sod then
attended the LSU School of Veterinary Medicine and obtained his DVM in 2001. He has since completed an equine internship
and food animal medicine and surgery residency at LSU and a large animal surgery residency in conjunction with the Sawtooth
Equine Service in Idaho and LSU. He is presently an assistant professor with the Farm Animal Medicine and Surgery service. Dr.
Sod received the American College of Veterinary Surgeons Research Publication Award in 2004 and 2006, the Mark S. Bloomberg
Memorial Resident Research Award from the Veterinary Orthopedic Society in 2004 and 2005, and the LSU Rainmaker Research
Award in 2009. His research interests include orthopedic surgery, biomechanics and the design of orthopedic implants specific
to the equine patient. He has designed 5 orthopedic plates, 1 interlocking intramedullary pin and 2 screws for use in long bone
fractures and joint arthrodesis in the equine patient. One screw design has been adopted by Acumed for human fracture patients.
2008-2010 EHSP FACULTY AND STAFF
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This work has resulted in the writing of a book on the biomechanics of fractures and fracture repair. He has tested and designed
orthopedic implants for numerous companies, including, Acumed, BioMedtrix, IMEX, Innovative Animal Products, Securos, and
Synthes. Synthes, Inc. has awarded Dr. Sod a 3 year grant for the design of equine specific orthopedic implants.
Eric Storey, Assistant Professor Ophthalmology, Veterinary Clinical Sciences
Dr. Storey is an assistant professor of ophthalmology in the Department of Veterinary Clinical Sciences and a veterinary
ophthalmologist in the Veterinary Teaching Hospital and Clinics. He received his BS and DVM from Auburn University in 1996
and 1999, respectively. Dr. Storey received his MVSc from University of Saskatchewan (Canada) in 2003. He completed
his ophthalmology internship in 2000 at the University of Illinois, and he completed his residency in 2003 at the University of
Saskatchewan. He is a Diplomate of the American College of Veterinary Ophthalmologists. His research interests are in glaucoma,
inherited retinal disease, and equine recurrent uveitis.
Changaram S. Venugopal, Professor, Veterinary Clinical Sciences
Dr. Venugopal is a veterinarian who graduated from Kerala Veterinary College and Research Institute of Kerala University, India.
After practicing as a veterinarian in the Kamadhenu Dairy Farm for five years, he pursued advanced studies and received a Master
of Science degree in neuropharmacology from Calicut University, India. Later he moved to the US for higher studies and received
another MS degree in cardiovascular pharmacology and a PhD in pulmonary pharmacology from the Massachusetts College
of Pharmacy and Allied Health Sciences in a cooperative program with Harvard University in Boston, MA. Then he worked as a
postdoctoral fellow at Harvard Medical School and, in 1981, joined the faculty at LSU School of Veterinary Medicine. He received
the New Investigator Award grant from the National Institutes of Health in 1983 and the Beecham Award for Research Excellence
in 1985. He is a recipient of Faculty Distinguished Scholar Award (2003) from LSU SVM and Distinguished Alumni Award from
his alma mater in 2005. His research interests include recurrent airway obstruction in horses, mediators of airway hyperreactivity,
pathophysiology of insulin resistance in equine laminitis, pharmacology of vasculature, and oxidative stress in equine respiratory
diseases. He is the recipient of research grants as principal investigator from NIH, USDA and Grayson Jockey Research
Foundation and grants from NIEHS and LA Board of Regents as co-PI. In the area of teaching, Dr. Venugopal received honor roll
in merit teaching 1994 to 1999 and the best teacher award (2008) by the class of 2011.
2008-2010 EHSP FACULTY AND STAFF
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TEXTBOOK CHAPTERS
ANDREWS
Nadeau J, Andrews F. Gastric Ulcer Syndrome. In Current Therapy in Equine Medicine Robinson NE, (eds) 6th Edition. Saunders
Co., St. Louis, MO. 366-371, 2009.
Reese RE, Andrews FM. Nutrition and Dietary Management of Equine Gastric Ulcer Syndrome. In Veterinary Clinics of North
America: Equine Practice 25(1):79-92, 2009.
Videla R, Andrews FM. New Perspectives in Equine Gastric Ulcer Syndrome. In Veterinary Clinics of North America: Equine
Practice 25(2):283-302, 2009.
Al Jassim RA, Andrews FM. The bacterial community of the horse gastrointestinal tract and its relation to fermentative acidosis,
laminitis, colic and stomach ulcers. Veterinary Clinics of North America: Equine Practice 25(2):199-215, 2009.
Andrews FM. Cerebrospinal fluid evaluation. In Equine Internal Medicine Reed SM, Bayly WM and Sellon, DC (eds), 3rd Edition
553-556, 2010.
Andrews FM, Lacombe VA. Electrodiagnostic aids and selected neurologic diseases. In Equine Internal Medicine Reed SM, Bayly
WM and Sellon, DC (Eds), 3rd Edition 556-567, 2010.
Andrews FM. Gastric ulcers in horses. The Merck Manual pages 197-198, 2010.
CHAPMAN
Chapman AM. Acute Diarrhea in hospitalized horses. Vet Clin North Am Equine Pract. 2009 Aug;25(2):363-80.
EADES
Eades SC. Overview of current laminitis research. Vet Clin North Am Equine Pract. 2010 Apr;26(1):51-63.
Stokes AM, Eades SC, Moore RM. Pathophysiology and Treatment of Acute Laminitis. Equine Internal Medicine 3rd Edition. Reed,
Bayly, Sellon. 2010.
EILTS
Mateus, L and Eilts, BE. Cystic Endometrial Hyperplasia and Pyometra In: Ettinger S. and Feldman, E (eds). Textbook Of
Veterinary Internal Medicine, 7th ed. 2209 pp. 1913-1921
Eilts, BE. Contraception and pregnancy termination in the dog and cat. In: Ettinger S. and Feldman, E (eds). Textbook Of
Veterinary Internal Medicine, 7th ed. 2209 pp. 1908-1913
Pinto CRF, Eilts BE, Paccamonti DL. Animal Reproduction In: Clinical Textbook for Veterinary Technicians, 7th edition, McCurnin
2008-2010 PUBLICATIONS AND ABSTRACTS
13
2008-2010 PUBLICATIONS AND ABSTRACTS
D and Bassert J, eds. Harcourt Health Sciences, St. Louis. 2009 370-399
GILL
Gill MS. Acute Renal Failure. In Haskell (ed): Five-Minute Veterinary Consult: Ruminant, 1st edition. Philadelphia, Lippincott,
Williams & Wilkins, March, 2009.
Gill MS. Amyloidosis. In Haskell (ed): Five-Minute Veterinary Consult: Ruminant, 1st edition. Philadelphia, Lippincott, Williams &
Wilkins, March, 2009.
Gill MS. Patent Urachus. In Haskell (ed): Five-Minute Veterinary Consult: Ruminant, 1st edition. Philadelphia, Lippincott, Williams &
Wilkins, March, 2009.
Gill MS. Pyelonephritis. In Haskell (ed): Five-Minute Veterinary Consult: Ruminant, 1st edition. Philadelphia, Lippincott, Williams &
Wilkins, March, 2009.
Gill MS. Floppy Kid Syndrome. In Haskell (ed): Five-Minute Veterinary Consult: Ruminant, 1st edition. Philadelphia, Lippincott,
Williams & Wilkins, March, 2009.
Gill MS. Penile Deviations. In Haskell (ed): Five-Minute Veterinary Consult: Ruminant, 1st edition. Philadelphia, Lippincott, Williams
& Wilkins, March, 2009.
Gill MS. Penile Hematoma. In Haskell (ed): Five-Minute Veterinary Consult: Ruminant, 1st edition. Philadelphia, Lippincott,
Williams & Wilkins, March, 2009.
Gill MS. Preputial Prolapse. In Haskell (ed): Five-Minute Veterinary Consult: Ruminant, 1st edition. Philadelphia, Lippincott,
Williams & Wilkins, March, 2009.
JOHNSON
Immunologic Disorders, Chapter 53. Consulting Editor, Large Animal Internal Medicine, ed. Smith B. St. Louis: Mosby Elsevier.
2009
Sellon DC, Hines MT, Johnson JR. Equine Immunodeficiency Diseases, in Large Animal Internal Medicine, ed. Smith B. St. Louis:
Mosby Elsevier. 2009, pp. 1665-1667
Johnson JR, Parish SM. Diseases caused by allogeneic incompatibilities in Large Animal Internal Medicine, ed. Smith B. St. Louis:
Mosby Elsevier. 2009, pp. 1682-1691.
LYLE
Lyle SK. Sexual development disorders. In: Tilley LP, Smith FWK (eds.) The 5-Minute Veterinary Consult: Canine and Feline, 4th
ed, Ames, IA, Blackwell Publishing, 2008.
Lyle SK. Vaginal malformations and acquired lesions. In: Tilley LP, Smith FWK (eds.), The 5-Minute Veterinary Consult: Canine
14
and Feline, 4th ed, Ames, IA, Blackwell Publishing, 2008.
Lyle SK. Infectious problems in the last trimester of pregnancy. In: Samper JC (ed.). Equine Breeding Management, 2nd ed.
Saunders Elsevier, Philadelphia PA; 2009; 249-254.
MCCONNICO
McConnico RS. Hubert J, Use of Slings in Equine Patients. In Current Therapy in Equine Medicine. 6th Ed., Robinson NE, ed. WB
Saunders Co., Philadelphia, PA., pp. 918-921, 2008.
McConnico RS. Acute Equine Colitis. In Current Therapy in Equine Medicine. 6th Ed., Robinson NE, ed WB Saunders Co,
Philadelphia, PA., pp 418-422, 2008.
McConnico RS. Emergency Equine Sheltering In: Veterinary Disaster Response, Eds. Wingfield WE, Palmer, SB. Blackwell
Publishing, Ames, IA, Ch 1.2 pp. 325-334. 2009.
McConnico RS, Equine review chapters (13) (restraint, behavior, nutrition, preventive medicine, infectious diseases, endocrine
disorders, hematology/ cardiology, gastrointestinal diseases, neurology, urology/nephrology, theriogenology, musculoskeletal
disorders, dentistry) In: Saunders Comprehensive Review for the NAVLE Board Review and Exam Practice Package, Schenck P,
Ed., Saunders, Philadelphia, PA October 2009.
MITCHELL
Mitchell CF, Leise B. Equine Surgery. In: Schenck PA (ed), Comprehensive Review for the NAVLE, 1st ed, St. Louis, Missouri,
Saunders/Elsevier, 2010, pp 495-538.
Mitchell CF, Moore RM, Gill MS. Large Animal Surgical Nursing. In: McCurnin DM, Bassert JM (eds), Clinical Textbook for
Veterinary Technicians, 7th ed, St. Louis, Missouri, Elsevier/Saunders, 2008).
PACCAMONTI
Pinto CRF, Paccamonti DL. Mare Reproductive Pathology. In: Disorders of the Reproductive Tract, Ch 18, Frazer G, ed., In:
Equine Internal Medicine, 3rd ed., Reed SM, Bayly WM, Sellon DC, eds. Saunders Elsevier, St. Louis, 2010, p 1016-1024.
Pinto CRF, Eilts BE, Paccamonti DL. Animal Reproduction. In: Clinical Textbook for Veterinary Technicians,7th ed. McCurnin DM
and Bassert JM, eds. Saunders Elsevier, St. Louis, 2010, p. 370--399.
Paccamonti D, Pycock J. Infertility and subfertility in the mare. In: Veterinary Reproduction and Obstetrics, 9th ed. Noakes DE,
Parkinson TJ and England GCW, eds. Saunders Elsevier, Edinburgh, 2009, p 582-631.
Paccamonti D, Parturition and Evaluation of the Placenta. In: Equine Breeding Management and Artificial Insemination, 2nd ed.
2008-2010 PUBLICATIONS AND ABSTRACTS
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2008-2010 PUBLICATIONS AND ABSTRACTS
Samper JC, ed., Saunders Elsevier, St. Louis, 2009, p 255-259.
Refereed Publications
ANDREWS
Andrews FM, Buchanan BR, Elliott SB, Al Jassim RAM, McGowen CM, and Saxton AM. In vitro effects of hydrochloric and lactic
acids on bioelectric properties of equine gastric squamous mucosa. Equine Veterinary Journal 40(3):301-305, 2008.
Kelmer G, Doherty TJ, Elliott S, Saxton A, Fry MM, and Andrews FM. Evaluation of dimethyl sulphoxide effects on initial response
to endotoxin in the horse. Equine Veterinary Journal 40(4):358-363, 2008.
Witonsky SG, Ellison S, Yang J, Gogal RM, Lawler H, Suzuki Y, Sriranganathan N, Andrews F, Ward D, Lindsay DS. Horses
experimentally infected with Sarcocystis neurona develop altered immune response in vitro. Journal of Parasitology 94(5):1047-
1054, 2008.
Andrews FM, Reinemeyer CR, Longhoffer SL. Effects of top-dress formulations of suxibuzone and phenylbutazone on
development of gastric ulcers in horses. Veterinary Therapeutics 10(3):113-120, 2009.
Peretich AL, Abbott LL, Andrews FM, Dhar MS. Age-dependent regulation of sodium-potassium adenosinetriphosphatase and
sodium-hydrogen exchanger RNAs in equine nonglandular mucosa. American Journal of Veterinary Research 70(9):1124-1128;
2009.
BURBA
Lewis AJ, Sod GA, Burba DJ. Relationship of Fracture Fragment Thickness to Interfragmentary Compression Following Placement
of 6.5-mm Headless Tapered Variable Pitch and 5.5-mm AO Cortical Bone Screws for Fixation of a Simulated Lateral Condylar
Fracture in Equine Third Metacarpal Bones. Veterinary Surgery.
Lewis AJ, Sod GA, Burba DJ, Mitchell CF. Compression of condylar fractures of varying fragment thickness with acutrak plus vs
4.5-mm ao cortical screws in equine third metacarpus. Veterinary Surgery, 39(1), 78-82, 2010.
EADES
Eades SC, Fugler LA, Riggs L. Controlling the equine neutrophil: A generator of devastating tissue damage during equine
inflammatory disease. Vet J. 2008 Oct;178(1):3-4
Costa LR, Eades SC, Venugopal CS, Moore RM. Plasma and pulmonary fluid endothelin in horses with seasonal recurrent airway
obstruction. J Vet Int Med. 23(6):1239-46, 2010
EILTS
Len JA, Jenkins JA, Eilts BE, Paccamonti DL, Lyle SK, Hosgood G. Immediate and delayed (after cooling) effects of centrifugation
on equine sperm. Theriogenology. 2010 Jan 15;73(2):225-31.
GILL
Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill MS. An in vitro biomechanical comparison of a 5.5 mm limited-contact dynamic
compression plate fixation with a 4.5 mm limited-contact dynamic compression plate fixation of osteotomized equine third
16
metacarpal bones. Veterinary Surgery. 37(3), 289-294, 2008.
Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill MS. An in vitro biomechanical comparison of a locking compression plate fixation
with a limited-contact dynamic compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Surgery.
37(3), 283-288, 2008.
Johnson SW, Gill MS, Grenier C, Taboada J. A descriptive analysis of personality and gender at the Louisiana State University
School of Veterinary Medicine. JVME. 35: 284-290, 2009.
Lewis AJ, Sod GA, Gill MS, Mitchell CF. Bovine distal interphalangeal joint arthrodesis using the acutrak plus screw: a report of
seven cases. Veterinary Surgery. 38(5), 659-663, 2009.
Sod GA, Riggs, LM, Mitchell CF, Martin GS, Gill MS. In vitro cyclic fatigue comparison of a modified 5.5 mm locking compression
plate fixation with a 5.5 mm locking compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Surgery,
39(7), 833-838, 2010.
Sod GA, Riggs, LM, Mitchell CF, Martin GS, Gill MS. An in vitro biomechanical comparison of a 5.5 mm locking compression plate
fixation with a 4.5 mm locking compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Surgery,
39(5), 581-587, 2010.
JOHNSON
Vidal MA, Robinson SL, Lopez MJ, Paulsen DB, Borkhsenious O, Johnson JR, Moore RM, Gimble JM. Comparison of
chondrogenic potential in equine mesenchymal stromal cells derived from adipose tissue and bone marrow. Vet Surgery 37:713-
724, 2008.
Hanly BK, Stokes AM, Bell AM, Johnson JR, Keowen ML, Paulsen DB, Sod GA, Moore RM. Use of serial laminar tissue collection
via biopsy in conscious healthy horses. Am J Vet R 70:697-702, 2009.
LOPEZ
Lopez MJ, McIntosh KR, Spencer ND, Borneman JN, Horswell R, Anderson P, Yu G, Gaschen L, Gimble JM. Acceleration of
spinal fusion using syngeneic and allogeneic adult adipose derived stem cells in a rat model. J Orthop Res 27:366-373, 2009.
Schaefer SL, Lu Y, Seeherman H, Li XJ, Lopez MJ, Markel, MD. The effect of rhBMP-2 on tibial plateau fractures in a canine
model. J Orthop Res 27:466-471, 2009.
McIntosh K, Lopez MJ, Borneman J, Spencer N, Anderson P, Gimble JM. Immunogenicity of allogeneic adipose-derived stem cells
in a rat spinal fusion model. Tissue Eng Part A 15:2677-86, 2009.
Lopez MJ, Davis KM, Jeffrey-Borger SL, Markel MD, Rettenmund C. Inter-observer repeatability of measurements on computed
tomography images of lax canine hip joints from youth to maturity. Vet Surg 38:920-6, 2009.
Snow LA, White R, Gustafson S, Xie L, Hosgood G, Monroe WT, Casey JP, Lopez MJ. Ex vivo comparison of three surgical
2008-2010 PUBLICATIONS AND ABSTRACTS
17
techniques to stabilize canine cranial cruciate ligament deficient stifles. Vet Surg 39:195-207, 2010.
Xie L, Spender ND, Beadle RE, Gaschen L, Buchert MR, Lopez MJ. Effects of athletic conditioning on horses with degenerative
suspensory ligament desmitis: A preliminary report. Vet Journal e-pub 6/2010.
LYLE
Lyle, S. K., Paccamonti, D. L. (2010). High risk pregnancy in the mare - practical implications for the practitioner. Pferdeheilkunde,
26(1), 29-35.
Paccamonti, D. L., Lyle, S. K. (2010). A Clinical View of Mare Reproductive Physiology. Pferdeheilkunde, 26(1), 10-13.
Len, J. A., Jenkins, J. A., Eilts, B. E., Paccamonti, D. L., Lyle, S. K., Hosgood, G. (2010). Immediate and delayed (after cooling)
effects of centrifugation on equine sperm. Theriogenology, 73(2), 225-231.
MCCAULEY
McCauley CT. Large Animal Emergency Nursing. In McCurnin DM, Bassert JM (eds) Textbook for Veterinary Technicians 7th Ed
7th ed, Elsevier, 2008 - 2009
MCCONNICO
Briere CA, Hosgood G, Morgan TW, Hedlund CS, Hicks M, McConnico RS. Effects of carprofen on the integrity and barrier function
of canine colonic mucosa. J Am Vet Med Assoc. 1;232(3):398. 2008
Briere CA, Hosgood G, Morgan TW, Hedlund CS, Hicks M, McConnico RS. Effects of carprofen on the integrity and barrier function
of canine colonic mucosa. Am J Vet Res.;69(2):174-81 2008
McConnico RS, Morgan TW, Williams CC, Hubert JD, Moore RM. Pathophysiologic effects of phenylbutazone on the right dorsal
colon in horses. Am J Vet Res. 2008 Nov;69(11):1496-505, 2009.
McConnico RS, Lunn DP, Review of controversial issues (nomenclature, case definition, diagnostic testing, and management
practices) in equine herpes myelitis (EHM), Proceedings of the 56th Annual Convention of the American Association of Equine
Practitioners Baltimore, MD, Dec. 5-8, 2010
MITCHELL
Gudehus T, Mirza MH, Mitchell CF. A milliliter of prevention. Compendium Equine. 3(1), 55-56, 2008.
Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill MS. An in vitro biomechanical comparison of a locking compression plate fixation
with a limited-contact dynamic compression plate fixation of osteotomized equine 3rd metacarpal bones. Veterinary Surgery. 37(3),
283-288, 2008.
Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill MS. An in vitro biomechanical comparison of a 5.5mm limited-contact dynamic
compression plate fixation with a 4.5mm limited-contact dynamic compression plate fixation of osteotomized equine 3rd metacarpal
2008-2010 PUBLICATIONS AND ABSTRACTS
18
bones. Veterinary Surgery. 37(3), 289-299, 2008.
Lewis AJ, Sod GA, Mitchell CF. Distal interphalangeal joint arthrodesis using the acutrak plus screw in 7 cattle. Veterinary Surgery.
38(5), 659-663, 2009.
Sod GA, Riggs, LM, Mitchell CF, Hubert JD, Martin GS. An in vitro biomechanical comparison of equine proximal interphalangeal
joint arthrodesis techniques: axially positioned dynamic compression plate and two abaxial transarticular cortical screws inserted in
lag fashion versus three parallel cortical screws inserted in lag fashion. Veterinary Surgery. 39(1), 83-90, 2010.
Lewis AJ, Sod GA, Burba DJ, Mitchell CF. Compression of condylar fractures of varying fragment thickness with acutrak plus vs
4.5-mm ao cortical screws in equine third metacarpus. Veterinary Surgery, 39(1), 78-82, 2010.
Sod GA, Riggs, LM, Mitchell CF, Martin GS, Gill MS. In vitro cyclic fatigue comparison of a modified 5.5 mm locking compression
plate fixation with a 5.5 mm locking compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Surgery,
39(7), 833-838, 2010.
Sod GA, Riggs, LM, Mitchell CF, Martin GS, Gill MS. An in vitro biomechanical comparison of a 5.5 mm locking compression plate
fixation with a 4.5 mm locking compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Surgery,
39(5), 581-587, 2010.
PACCAMONTI
Paccamonti DL, Carnevale E. Early embryonic death in mares. Clinical Theriogenology. 2:99-110, 2010.
Paccamonti DL, Lyle SK. A clinical view of mare reproductive physiology. Pferdeheilkunde. 26:10-13, 2010.
Lyle SK, Paccamonti DL. High risk pregnancy in the mare – practical implications for the practitioner. Pferdeheilkunde. 26:29-35,
2010.
Immediate and delayed (after cooling) effects of centrifugation on equine sperm. Len, J.A., Jenkins, J.A., Eilts, B.E., Paccamonti,
D.L., Lyle, S.K., Hosgood, G. Theriogenology, 73:225-231, 2010.
Wirtu, G., Pope, C.E., Paccamonti, D.L., Godke, Robert. A., Dresser, B.L. Ultrasonographic-guided retrieval and in vitro maturation
of eland (Taurotragus oryx) and bongo (Tragelaphus eurycerus isaaci) antelope oocytes. Animal Reproduction Science 111:160-
172, 2009.
Parlevliet, J.M., Paccamonti, D.L., Barker, S.A. Cefquinome concentrations in endometrium after intrauterine treatment of cobactan
4.5%® in mares and inflammatory response of the endometrium to this treatment. Reprod Dom Anim 44:189-193, 2009
Wirtu G, Pope CE, MacLean RA, Godke RA, Paccamonti DL, Dresser BL. Reversal of motility loss in bongo antelope (Tragelaphus
eurycerus isaaci) spermatozoa contaminated with hyposmotic urine during electroejaculation. Animal Reproduction Science
103:392-397, 2008.
2008-2010 PUBLICATIONS AND ABSTRACTS
19
Paccamonti DL, Carnevale E, Pregnancy loss during the embryonic period. Pferdeheilkunde. 24(1):92-94, 2008.
SOD
Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill. MS. An in vitro biomechanical comparison of a locking compression plate fixation
with a limited-contact dynamic compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Surgery.
37(3), 283-288, 2008.
Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill. MS. An in vitro biomechanical comparison of a 5.5 mm limited-contact dynamic
compression plate fixation with a 4.5 mm limited-contact dynamic compression plate fixation of osteotomized equine third
metacarpal bones. Veterinary Surgery. 37(3), 289-299, 2008.
Hanly BK, Stokes AM, Bell AM, Johnson JR, Keowen ML, Paulsen DB, Sod GA, Moore RM. Use of serial laminar tissue collection
via biopsy in conscious healthy American Journal of Veterinary Research. 70(6), 697-702, 2009.
Lewis AJ, Sod GA, Mitchell CF. Distal interphalangeal joint arthrodesis using the acutrak plus screw in 7 cattle. Veterinary Surgery.
38(5), 659-663, 2009.
Sod GA, Riggs, LM, Mitchell CF, Martin GS, Gill MS. In vitro cyclic fatigue comparison of a modified 5.5 mm locking compression
plate fixation with a 5.5 mm locking compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Surgery,
39(7), 833-838, 2010.
Sod GA, Riggs, LM, Mitchell CF, Martin GS, Gill MS. An in vitro biomechanical comparison of a 5.5 mm locking compression plate
fixation with a 4.5 mm locking compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Surgery,
39(5), 581-587, 2010.
Sod GA, Riggs, LM, Mitchell CF, Hubert JD, Martin GS. An in vitro biomechanical comparison of equine proximal interphalangeal
joint arthrodesis techniques: axially positioned dynamic compression plate and two abaxial transarticular cortical screws inserted in
lag fashion versus three parallel cortical screws inserted in lag fashion. Veterinary Surgery. 39(1), 83-90, 2010.
Lewis AJ, Sod GA, Burba DJ, Mitchell CF. Compression of condylar fractures of varying fragment thickness with acutrak plus vs
4.5-mm ao cortical screws in equine third metacarpus. Veterinary Surgery, 39(1), 78-82, 2010.
VENUGOPAL
Venugopal, C.S., Holmes, E. P., Polikepahad, S., Laborde, S., Kearney, M., Moore, R.M. Neurokinin receptors in recurrent airway
obstruction: A comparative study of affected and unaffected horses. Can. J. Vet., Res., 73(1): 25-33, 2009.
Costa, L.R., Eades, S.C., Venugopal, C.S. Moore, R.M. Plasma and pulmonary fluid endothelin in horses with seasonal recurrent
airway obstruction. J Vet Intl. Med., 23: 12139-1246, 2009.
2008-2010 PUBLICATIONS AND ABSTRACTS
20
2008-2010 PUBLICATIONS AND ABSTRACTS
Non Refereed Publications
ANDREWS
Andrews F, Denovo R, Reese R, Elliott S, Moyers T, Barthel D, Lyman M, Daniel G. The Evaluation of the wireless SmartPill™ for
measuring gastric emptying and GI transit in normal dogs. Journal of Veterinary Internal Medicine 2008; 751.
Buchanan B, Andrews F, Elliott S, Al Jassim R, McGowan C, Saxton A. In vitro effects of lactic acid on bioelectric properties of
equine nonglandular squamous mucosa. Journal of Veterinary Internal Medicine 2008; 815.
Reese R, Andrews F, Elliott S, Saxton A, McMullin RB. The effect of Seabuckthorn extract in the treatment and prevention of
gastric ulcers in horses. Journal of Veterinary Internal Medicine 2008; 815.
Elliott S, Reese R, Denovo R, Barthel D, Lyman M, Danien G, Andrews, F. Use of a wireless capsule, SmartPill™ to measure
gastrointestinal pH, pressure and transit time in a horse. Journal of Veterinary Internal Medicine 2008; 815-816.
Andrews F, McConnico R. Cause of concern: Evidence that therapeutic dosing of nonselective NSAIDs contributes to
gastrointestinal injury (Clinical Commentary). Equine Veterinary Education 21(12):663-664, 2009.
BURBA
Burba DJ. Can cribbing be halted? The Horse. November 2008.
CHAPMAN
Eades SC and Chapman AM. Foal diarrhea and Pneumonia. Louisiana Horse Magazine, Spring 2010.
Chapman AM. Lawsonia intracellularis: An emerging pathogen in weanling foals. Louisiana Horse Magazine, Fall 2009.
MCCAULEY
McCauley CT. Cryptorchid Castration and Scrotal Ablation in Horses. DVM Magazine 2009.
MCCONNICO
McConnico RS, Preparing an equine practice for a natural disaster, Commentary DVM Magazine, Veterinarynews360, Dec 1, 2008
McConnico RS, Beware Bute’s Adverse Effects, Researchers Recommend. The Horse.com, Article # 13073 November 12, 2008
McConnico RS, Microchipping, Appaloosa Journal. Feb 2008
McConnico RS. Our responsibility for disaster preparedness: Fighting the “It won’t ever happen to me” attitude. Newsletter for the
American Association of Human Animal Bond Veterinarians, Spring 2008
Andrews FA, McConnico RS, Clinical Commentary: Cause for concern: Evidence that therapeutic dosing of non-selective NSAIDs
contributes to gastrointestinal injury. Eq Vet Edu. 21(12) pp. 663-64, Dec 2009
21
2008-2010 PUBLICATIONS AND ABSTRACTS
McConnico RS, Blood tests may detect early NSAID damage, Equus 377, p 10. Feb 2009
McConnico RS, BUTE: Not the Aspirin You Thought it Was, www.grayson-jockeyclub.org/news, Summer 2009
McConnico RS, Disaster Preparedness for the Horse Owner: Horse TV Stories, March 11, 2009
McConnico RS, Sykes M, Bute: Not the Aspirin You Thought It Was The Horsemen’s Journal, http://www.horseracingofficials.com ,
June 22, 2009
McConnico RS, Merial: Equine - Know the Signs of an NSAID Overdose, Cattlenetwork.com, 7/30/09
McConnico RS, LSART rescues horse from flood, The Daily Reveille, LSUReveille.com, Nov. 17, 2009
McConnico RS LSART rescues horse from flood, The Horse.com Nov 23, 2009
Equine Herpes Virus-1: Minimizing Costs, Dispelling Myths (AAEP 2010); December 2010
McConnico RS, Beware Bute’s Adverse Effects, Researchers Recommend. TheHorse.com, November 12 2008, Article # 13073
PACCAMONTI
Paccamonti DL. Leipziger Blaue Hefte - Proc. 5th Leipziger Tierarztekongress. Understanding the mare’s estrous cycle. P 236-240.
Paccamonti D, Mare Research Update. Clinical Theriogenology, 1:335-336, 2009.
Paccamonti D. Reproductive physiology and cyclicity in the mare from a clinical viewpoint; Insemination strategies in the mare.
Proc. Ann Mtg Vet. Med. Soc. Denmark, Copenhagen, 2008
Paccamonti D. Multiple ovulation and twinning; Fecundacion y gestacion temprana de la yegua (< 60 dias) y Manejo de la yegua
gestante y gestaciones gemelares. Proc. Curso Diplomado en Reproducion, Guadalajara, 2008
Abstracts
ANDREWS
Andrews F, Denovo R, Reese R, Elliott S, Moyers T, Barthel D, Lyman M, Daniel G. The Evaluation of the wireless SmartPill™ for
measuring gastric emptying and GI transit in normal dogs. Journal of Veterinary Internal Medicine 2008; 751.
Buchanan B, Andrews F, Elliott S, Al Jassim R, McGowan C, Saxton A. In vitro effects of lactic acid on bioelectric properties of
equine nonglandular squamous mucosa. Journal of Veterinary Internal Medicine 2008; 815.
Reese R, Andrews F, Elliott S, Saxton A, McMullin RB. The effect of Seabuckthorn extract in the treatment and prevention of
gastric ulcers in horses. Journal of Veterinary Internal Medicine 2008; 815.
22
2008-2010 PUBLICATIONS AND ABSTRACTS
Elliott S, Reese R, Denovo R, Barthel D, Lyman M, Danien G, Andrews, F. Use of a wireless capsule, SmartPill™ to measure
gastrointestinal pH, pressure and transit time in a horse. Journal of Veterinary Internal Medicine 2008; 815-816.
Reese RE, Andrews FM, Elliott SB, Saxton AM. Effect of volatile fatty acid mixes on equine nonglandular mucosa bioelectric
properties: Pathogenesis of gastric ulcers in horses. Journal of Veterinary Internal Medicine 2009; 700.
Andrews F, A Peretich, RE Reese, L Abbott, M Dhar. Role of cellular sodium transport in nonglandular equine gastric ulcer
disease. Journal of Veterinary Internal Medicine 2010; 24:792 (Abstract #399)
BEADLE
Mitchell CF, Beadle RE. Deep digital flexor tenotomy as a treatment for horses with chronic laminitis. Poster presentation at the
2nd AAEP Foundation Equine Laminitis Research Workshop, West Palm Beach, Florida. November 8-10, 2009.
Venugopal CS, Beadle RE, Holmes EP et al. Induction of insulin resistance in equine digital vessel rings [abstract]. J Vet Intern
Med 2010;24(3):790.
CHAPMAN
Buchert M, Gebhart C, Eades S, Johnson J, Chapman A. Identification of exposure to and shedding of Lawsonia intracellularis
in Louisiana horses using serology and real time PCR. Phi Zeta Research Day, LSU SVM, Baton Rouge, LA, September 2010.
(Poster Presentation).
Chapman AM. Identification of exposure to and shedding of Lawsonia intracellularis in Louisiana horses using serology and real
time PCR. First Annual Lawsonia intracellularis workshop. Haygard Medical Institute, Lexington, KY, June 25, 2009.
Johnson JR, Eades SC, Chapman A, Hosgood G. Implementing a geographical information system (GIS) to investigate nosocomial
Salmonella infections. 2nd International Symposium on Geospatial Health, New Orleans, LA, December 2008.
Johnson JR, Eades SC, Chapman AM, Truman C, Parker J. Temporospatial tracking of Salmonella infections in hospitalized
equine patients in a veterinary teaching hospital. 3rd Intl Symp on Geospatial Health, Veitri Sul Mare, Italy, September 2009.
Truman C, Johnson JR, Eades SC, Chapman AM, Parker J. Salmonellosis in LSU Equine Hospital 8/31/2000 to 5/19/2009. Phi
Zeta Research Emphasis Day, September 23, 2009, Baton Rouge, LA.
Thompson JA; Eades SC, Chapman AM, Barker SA; Paulsen DB; McConnico RS, Assessment of Cardiac Function, Skeletal
Muscle Morphology, and Muscle Enzymes in Horses on Long-Term Clenbuterol Therapy, Proceedings of the ACVIM Annual
Forum, Montreal, Quebec, Canada, June 3-6, 2009
EADES
Fugler LA, Eades SC. Clinical and Matrix Metalloproteinase Inhibitory Effects of Pentoxifylline on carbohydrate overload laminitis:
preliminary results. J Equine Vet Sci. 30:106, 2010.
23
2008-2010 PUBLICATIONS AND ABSTRACTS
EILTS
Len JA, Jenkins JA, Eilts B.E, Paccamonti DL, Lyle SK, Hosgood G. Centrifugation has minimal effects on motility,viability, and
acrosome integrity of equine sperm. Theriogenology 70: 582-583, 2008.
Jill A. Jenkins, Bruce E. Eilts, and Rassa O. Draugelis-Dale. Tools and Techniques Flow Cytometry: An Essential Tool in Biomarker
Research in Briggs, K.M., ed., 2010, Proceedings of the U.S. Geological Survey Interdisciplinary Microbiology Workshop, Estes
Park, Colorado, October 15–17, 2008: U.S. Geological Survey Scientific Investigations p 35.
Lyle, SK, Gentry, LR, Horohov, DW, Johnson, JR, Eilts, BE, Godke, RA, Paccamonti, DL. Preliminary evidence of fetal
hypothalamic-pituitary-adrenal axis activation in an experimental model of infective preterm delivery in the mare. Clinical
Theriogenology. Volume 1 Number 1. 2009. P 238.
Eilts BE, Pennington PM, Vermeulen MAE, Pope CE, Lyle SK, Len JA, Godke RA. Comparing Deslorelin Implants or Oral
Diethylstilbestrol to Induce Estrus in the Bitch. Clinical Theriogenology (2010):2 p 380.
Vermeulen MAE, Eilts BE, Hosgood G, Rademacher N, Pennington PM, Lyle SK, Len JA, Godke RA, Pope CE, Parlevliet JM.
Ovarian Color-Doppler Ultrasonography to Predict Ovulation in the Bitch. Clinical Theriogenology (2010):2 p 356.
Len JA, Vermeulen MAE, Eilts BE, Lyle SK. Low Dose Prostaglandin F2α for Luteal Regression in the Bitch. Clinical
Theriogenology (2010):2 p 362.
Beehan DP, Lyle SK, Len JA, Staempfli SA, Eilts BE. Investigation of preovulatory serum progesterone increase after hCG
treatment in mares. Animal Reproduction Science 121S (2010) S49–S50.
Causey RC, Calderwood Mays MB, Anderson KJ, Lyle SK, Paccamonti DL, Eilts BE, LeBlanc MM. Alteration of the uterine
epithelium in chronically infertile mares. Animal Reproduction Science 121S (2010) S92–S93.
Lyle SK, Hague M, Lopez MJ, Beehan DP, Staempfli SA, Len JA, Eilts BE, Paccamonti DL. In vitro production of cortisol by equine
fetal adrenal cells in response to ACTH and IL-1. Animal Reproduction Science 121S (2010) S322–S323.
GILL
Sod GA, Riggs, LM, Mitchell CF, Martin GS, Gill MS. An in vitro biomechanical comparison of a 5.5 mm locking compression plate
fixation with a 4.5 mm locking compression plate fixation of ostectomized equine third metacarpal bones. Veterinary Orthopedic
Society 36th Annual Conference, Steamboat Springs, CO, February, 2009.
Gudehus TH, Sod GA, Mitchell CF, McCauley CT, Gill MS. An in vitro analysis of temperatures and insertion torques of HA coated
and uncoated 5.5 mm AO cortical screws. Veterinary Orthopedic Society 36th Annual Conference, Steamboat Springs, CO,
February, 2009.
JOHNSON
Schur D, Rademacher N, McCauley C, Lopez M, Spencer N, Vidal M, Burba D, Johnson J, , Gaschen L,. Ultrasound assessment
protocol and preliminary findings of equine tendon injury before and after stem cell therapy. Phi Zeta Day, LSU SVM, Baton Rouge,
LA, September 2008.
24
2008-2010 PUBLICATIONS AND ABSTRACTS
Vidal MA, Robinson SO, Lopez MJ, Paulsen DB, Johnson JR, Moore RM, Gimble JM. Comparison of chondrogenic potential in
mesenchymal stromal cells derived from adipose tissue and bone marrow. Vet Orthopedic Society, 2008.
Johnson JR, Eades SC, Chapman A, Hosgood G. Implementing a geographical information system (GIS) to investigate nosocomial
Salmonella infections. 2nd International Symposium on Geospatial Health, New Orleans, LA, December 2008.
Johnson JR, Eades SC, Chapman AM, Truman C, Parker J. Temporospatial tracking of Salmonella infections in hospitalized
equine patients in a veterinary teaching hospital. 3rd Intl Symp on Geospatial Health, Veitri Sul Mare, Italy, September 2009.
Truman C, Johnson JR, Eades SC, Chapman AM, Parker J. Salmonellosis in LSU Equine Hospital 8/31/2000 to 5/19/2009. Phi
Zeta Research Emphasis Day, September 23, 2009, Baton Rouge, LA.
EHSP Seminar, Implementing a GIS to study nosocomial salmonella in a veterinary teaching hospital. September 2009.
Chapman AM, Eades SC, Johnson JR, Gebhardt C. Identification of exposure to and shedding of Lawsonia intracellularis in
Louisiana horses using serology and real-time PCR.
Lyle SK, Gentry LR, Horohov DW, Johnson JR, Eilts Be, Godkey RA, Paccamonti DL. Preliminary evidence of fetal hypothalamic-
ituitary-adrenal axis activation in an experimental model of infective preterm delivery in a mare. Therio/ACT Annual Conference,
August 20009, Albuquerque, New Mexico.
LOPEZ
Xie L, Robinson S, Spencer N, Buchert M, Gaschen L, Lopez MJ. Effects of athletic conditioning on equine degenerative
suspensory ligament desmitis. American Association of Equine Practitioners, 2009.
Huff N, Spencer N, Gimble J, Bagby G, Nelson S, Lopez MJ. Impaired multipotential capacity of adult stem cells in a rat chronic
alcohol abuse model. Proceedings American College of Veterinary Surgeons Annual Symposium, 2009.
Lopez MJ, Spencer ND, Robinson S, Burba D, Miska T. Effects of monopolar radiofrequency energy and diode laser energy on
equine distal tarsal joint articular cartilage. Proceedings American College of Veterinary Surgeons Annual Symposium, 2009.
Lopez MJ, Spencer ND, Robinson S, Holliday J. Osteoprotegerin and receptor activator of nuclear factor kappa beta ligand
expression in normal and osteoarthritic canine hip joint articular cartilage. Proceedings American College of Veterinary Surgeons
Annual Symposium, 2009.
Lopez M. Effect of chronic alcohol consumption on adult mesenchymal stem cells. Proceedings Research Society on Alcoholism
Scientific Meeting, 2009.
Lopez MJ, Huff NK, Spencer ND, Nelson S, Bagby GJ, Gimble JM. Alterations in adult mesenchymal stromal cell growth,
expansion, and pluripotential associated with chronic alcohol consumption. Alcoholism 33:32A, 2009.
Ayer K, Patterson D, Lopez MJ, Murphy MC, Measuring skeletal kinematics with accelerometers on the skin surface, Proceedings
American Society of Mechanical Engineers, 2009.
25
2008-2010 PUBLICATIONS AND ABSTRACTS
Lopez MJ, Spencer N, Robinson S. Osteoprotegerin and receptor activator of nuclear factor kappa beta ligand expression in
normal and osteoarthritic hip joint articular cartilage in a canine model. Proceedings Orthopaedic Research Society E2205, 2009.
Spencer N, Chun R, Vidal MA, Gimble JM, Lopez MJ. In vitro comparison of adult canine bone marrow- and adipose derived
stromal cell growth characteristics, Proceedings 44th Annual Symposium American College of Veterinary Surgeons, 2010.
Lopez MJ. Breaking news on adult canine stem cells. Proceedings American Veterinary Medical Association Annual Convention,
2010.
Bragulla H, Foster AL, Lopez MJ. Soft and hard keratins in the hooves of equine fetuses and adult horses. XXVIIIth Congress of
the European Association of Veterinary Anatomists, 2010.
Lopez MJ, Huff N, Spencer J, Bagby G, Nelson S, Gimble J. Chronic Alcoholism Reduces Adult Stem Cell Function. Proceedings
56th Annual Meeting Orthopaedic Research Society, 2010.
LYLE
Bradecamp EA, Snyder S, Rocha A, Lyle SK, Len J, Eilts BE, Godke RA, Paccamonti DL. Can time of day of ovulation be
influenced by hCG? . Pferdeheilkunde 24:112, 2008. (abstr)
Mocklin CM, Lyle SK, Eilts BE, Wouters EGH, Gentry LR, Godke RA, Paccamonti DL. Is the individual variation in response to
post-ovulatory cloprostenol in mares due in part to timing of initiation of treatment in relation to ovulation? Pferdeheilkunde 24:112,
2008. (abstr)
Lyle SK, Gentry LR, Horohov DW, Johnson JR, Eilts BE, Godke RA, Paccamonti DL. Preliminary evidence of fetal hypothalamic-
pituitary-adrenal axis activation in an experimental model of infective preterm delivery in the mare. Clinical Theriogenology. Volume
1 Number 1. 2009. P 238.
Causey RC, Mays MM, Anderson KJ, Lyle SK, Paccamonti DL, Eilts BE, LeBlanc MM. (2010). Alteration of the uterine epithelium
in chronically infertile mares Anim Repro Sci 121 Supp 1-2: 92-93).
Staempfli SA, Clavier S, Beehan DP, Lyle SK, Thompson DL, Burns PJ, McKinnon AO. (2010). Effect of a single injection of long-
acting progesterone on first ovulation in early and late transitional mares. Anim Repro Sci 121 Supp 1-2: 26-27.
Lyle SK, Hague MJ, Lopez MJ, Beehan DP, Staempfli S, Eilts BE, Paccamonti DL. (2010). In vitro production of cortisol by equine
fetal adrenal cells in response to ACTH and IL-1α. Anim Repro Sci 121 Supp 1-2: 322-323.
Beehan DP, Lyle SK, Len JA, Staempfli SA, Eilts BE. (2010). Investigation of preovulatory progesterone increase after hCG
treatment in mares. Anim Repro Sci 121 Supp 1-2: 49-50.
Lyle, S. K., Gentry, L. R., Johnson, J. R., Eilts, B. E., Godke, R. A., Paccamonti, D. L. (2010). Maturation of the equine fetal
hypothalamic pituitary axis in response to in utero inflammation. Pferdeheilkunde 26(1): 47.
26
2008-2010 PUBLICATIONS AND ABSTRACTS
Gentry, L. R., Gentry, G., Bonura, A., Staempfli, S. A., Len, J. A., Lyle, S. K., Burns, P., Paccamonti, D. L. (2010). Suppression of
follicular activity in mares using high dose estrogen. Pferdeheilkunde 26(1):28.
MCCONNICO
Hayden M, McConnico RS, Barker S, Comparative Metabolism of Levamisole in Horses and Cattle: Formation of the Stimulant
Drug Aminorex, Merial Summer Veterinary Student Honors Research Symposium, Michigan State University, East Lansing, MI,
2008
McConnico RS, Eller W, The Role of the Veterinarian in Community Disaster Planning and Response, U.S. Dept of Health &
Human Services National Disaster Medical Systems Training Summit, Nashville, TN May 15-18, 2008
Rigney H, Poirrier RA, McConnico RS, Implementation of the PETS Act with USDA-APHIS Officials and Louisiana Dept. of
Agriculture & Forestry, U.S. Dept of Health & Human Services National Disaster Medical Systems Training Summit, Nashville, TN
May 15-18, 2008
McConnico RS, Roy AF, Eades SC, Andrews F, Comparison of PCR Testing for Management of an Outbreak of EHVI at a
Racetrack, Proceedings of the ACVIM Annual Forum, Montreal, Quebec, Canada, June 3-6, 2009
Thompson JA; Eades SC, Chapman AM, Barker SA; Paulsen DB; McConnico RS, Assessment of Cardiac Function, Skeletal
Muscle Morphology, and Muscle Enzymes in Horses on Long-Term Clenbuterol Therapy, Proceedings of the ACVIM Annual
Forum, Montreal, Quebec, Canada, June 3-6, 2009
McConnico RS, The Role of the Veterinarian in Disaster Animal Response, Proceedings of the USDA Foreign Animal and
Emerging Diseases (FAED) Symposium, University of Tennessee, Knoxville, TN, August 3-7, 2009
Khodadad J, McConnico RS, Examining the pharmacokinetics and safety of firocoxib, a COX-1 sparing NSAID in foals Merial
Summer Scholars Program, National Symposium, NCSU-CVM, Raleigh, NC, Aug 2009
McConnico RS, The Role of the Veterinarian in Disaster Animal Response, Proceedings of Purina’s Equine Veterinary Conference,
Purina Mills, Inc., St. Louis, MO, October 2-4, 2009
Sousa D, Poirrier R, Adcock RA, McConnico RS, The impact of animals on evacuation management, Proceedings of the National
Evacuation Conference, New Orleans, LA, Feb 3-5, 2010
McConnico RS, Navarre CB, Disaster Preparedness: All Hazards/All Animals.
Proceedings of the Southwest Veterinary Symposium, Fort Worth, Texas, September 23 – 26, 2010
McConnico RS, Equine Disaster Preparedness & Mitigation Proceedings of the Southwest Veterinary Symposium, Fort Worth,
Texas, September 23 – 26, 2010
Fernandez RR, Koch C, McConnico RS, Effects of Buscopan and flunixin on permeability and secretory capacity of equine right
dorsal colonic mucosa, Merial Summer Scholars Program, Merial Summer Scholars Program, National Symposium, Athens, GA,
Aug, 2010
27
2008-2010 PUBLICATIONS AND ABSTRACTS
MITCHELL
Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill MS. Why do orthopedic implants fail in equine long bone fracture patients? – Part
1. Veterinary Orthopedic Society 35th Annual Conference, Big Sky, MT, March 2008.
Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill MS. Why do orthopedic implants fail in equine long bone fracture patients? – Part
2. Veterinary Orthopedic Society 35th Annual Conference, Big Sky, MT, March 2008.
Lewis AJ, Sod GA, Burba DJ, Mitchell CF. Compression of fracture fragments of varying thickness with acutrak plus or 4.5mm ao
cortical screws in equine 3rd metacarpal condylar fractures. Veterinary Orthopedic Society 35th Annual Conference, Big Sky, MT,
March 2008.
Lewis AJ, Sod GA, Mitchell CF. Bovine distal interphalangeal joint arthrodesis using the acutrak plus screw. Veterinary Orthopedic
Society 35th Annual Conference, Big Sky, MT, March 2008.
Mitchell CF, Beadle RE. Deep digital flexor tenotomy as a treatment for horses with chronic laminitis. Poster presentation at the
2nd AAEP Foundation Equine Laminitis Research Workshop, West Palm Beach, Florida. November 8-10, 2009.
Gudehus TH, Sod, GA, Mitchell CF, MCCauley CT. An in vitro analysis of temperatures and insertion torques of HA coated and
uncoated 5.5 mm AO cortical screws. Proceedings of the 44th Annual American College of Veterinary Surgeons Symposium,
Washington, D.C., October 2009.
Sod GA, Riggs, LM, Mitchell CF, Martin GS, Gill MS. An in vitro biomechanical comparison of a 5.5 mm locking compression plate
fixation with a 4.5 mm locking compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Orthopedic
Society 36th Annual Conference, Steamboat Springs, CO, February 2009.
Gudehus TH, Sod, GA, Mitchell CF, McCauley CT, Gill MS. An in vitro analysis of temperatures and insertion torques of HA coated
and uncoated 5.5 mm AO cortical screws. Veterinary Orthopedic Society 36th Annual Conference, Steamboat Springs, CO,
February 2009.
Sod GA, Riggs, LM, Mitchell CF, Martin GS. An in vitro biomechanical comparison of equine proximal interphalangeal joint
arthrodesis techniques: a new axial positioned LCP versus an axial positioned DCP each with two abaxial transarticular cortical
screws inserted in lag fashion. Veterinary Orthopedic Society 37th Annual Conference, Breckenridge, CO, February 2010.
PACCAMONTI
Causey RC, Calderwood Mays MB, Anderson KJ, Lyle SK, Paccamonti DL, Eilts BE, LeBlanc MM. Alteration of the uterine
epithelium in chronically infertile mares. Anim Reprod Sci 121S:S92-S93, 2010.
Lyle SK, Hague M, Lopez MJ, Beehan DP, Staempfli S, Len J, Eilts BE, Paccamonti DL. In vitro production of cortisol by equine
fetal adrenal cells in response to ACTH and IL-1β. Anim Reprod Sci 121S:S322-S323, 2010.
Gentry L, Gentry G, Bonura A, Staempfli S, Len J, Lyle S. Burns P, Paccamonti D. Suppression of follicular activity in mares using
high dose estrogen. Pferdeheilkunde.26:28, 2010.
28
2008-2010 PUBLICATIONS AND ABSTRACTS
Lyle SK, Gentry LR, Horohov DW, Johnson JR, Eilts BE, Godke RA, Paccamonti DL. Maturation of the fetal hypothalamic pituitary
axis in response to in utero inflammation. Pferdeheilkunde. 26:47, 2010.
Lyle SK, Gentry LR, Horohov DW, Johnson JR, Eilts BE, Godke RA, Paccamonti DL. Preliminary evidence of fetal hypothalamic-
pituitary-adrenal axis activation in an experimental model of infective preterm delivery in the mare. Clinical Theriogenology. 1:238,
2009.
Lyle SK, Gentry LR, Horohov DW, Johnson JR, Eilts BE, Godke RA, Paccamonti DL. Preliminary evidence of fetal cortisol
production in response to inflammation in a model of equine placentitis. Proc Am Assoc Eq Pract, 55: 264-265, 2009.
Len JA, Jenkins JA, Eilts BE, Paccamonti DL, Lyle SK, Hosgood G. Centrifugation has minimal effect on motility, viability, and
acrosome integrity of equine sperm. Theriogenology 70:582-583, 2008.
Mocklin CM, Lyle SK, Eilts BE, Wouters EGH, Gentry LR, Godke RA, Paccamonti DL. Is the individual variation in response to
post-ovulatory cloprostenol in mares due in part to timing of initiation of treatment in relation to ovulation? Pferdeheilkunde, 24:112,
2008.
Bradecamp EA, Snyder A, Rocha A, Lyle SK, Len J, Eilts BE, Godke RA, Paccamonti DL. Can time of day of ovulation be
influenced by hCG? Pferdeheilkunde 24:112, 2008.
Saenz JR, Guerrero C, Paccamonti D, Eilts B, Bondioli K, Godke RA. Processing of postmortem bovine epididymal sperm after
cooling the testes for 24 Hours. Proc Intl Emb Transf Soci, 2008.
SOD
Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill MS. Why do orthopedic implants fail in equine long bone fracture patients? - Part I.
Veterinary Orthopedic Society 35th Annual Conference, Big Sky, MT, March 2008.
Sod GA, Mitchell CF, Hubert JD, Martin GS, Gill MS. Why do orthopedic implants fail in equine long bone fracture patients? – Part
II. Veterinary Orthopedic Society 35th Annual Conference, Big Sky, MT, March 2008.
Lewis AJ, Sod GA, Burba DJ, Mitchell CF. Compression of fracture fragments of varying thickness with acutrak plus or 4.5mm ao
cortical screws in equine third metacarpal condylar fractures. Veterinary Orthopedic Society 35th Annual Conference, Big Sky, MT,
March 2008.
Lewis AJ, Sod GA, Mitchell CF. Bovine distal interphalangeal joint arthrodesis using the acutrak plus screw. Veterinary Orthopedic
Society 35th Annual Conference, Big Sky, MT, March 2008.
Durham ME, Sod GA, Lewis AJ. An in vitro analysis of insertion characteristics of hydroxyapatite-coated and uncoated positive
profile transfixation pins using a stepped drill bit in the third metacarpal bone of horses. Proceedings of the 44th Annual American
College of Veterinary Surgeons Symposium, Washington, D.C., October 2009.
Durham ME, Sod GA, Riggs LM, Lewis AJ. Laparoscopic comparison of adhesion formation associated with placement of a
macroporous condensed polytetrafluoroethylene mesh vs. polypropylene mesh in the equine abdomen. Proceedings of the 44th
29
2008-2010 PUBLICATIONS AND ABSTRACTS
Annual American College of Veterinary Surgeons Symposium, Washington, D.C., October 2009.
Sod GA, Riggs, LM, Mitchell CF, Martin GS, Gill MS. An in vitro biomechanical comparison of a 5.5 mm locking compression plate
fixation with a 4.5 mm locking compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Orthopedic
Society 36th Annual Conference, Steamboat Springs, CO, February 2009.
Gudehus TH, Sod, GA, Mitchell CF, McCauley CT, Gill MS. An in vitro analysis of temperatures and insertion torques of HA coated
and uncoated 5.5 mm AO cortical screws. Veterinary Orthopedic Society 36th Annual Conference, Steamboat Springs, CO,
February 2009.
Sod GA, Riggs, LM, Mitchell CF, Martin GS. An in vitro biomechanical comparison of equine proximal interphalangeal joint
arthrodesis techniques: a new axial positioned lcp versus an axial positioned dcp each with two abaxial transarticular cortical
screws inserted in lag fashion. Veterinary Orthopedic Society 37th Annual Conference, Breckenridge, CO, February 2010.
Sod GA and Vidal MA. An assessment of locking and AO screw position to increase cyclic fatigue during axial compression of an
oblique mid-diaphyseal equine third metacarpal fracture model. 12th Biennial Conference of the International Society for Fracture
Repair, London, England, September 2010.
VENUGOPAL
Venugopal CS, Mariappan N, Holmes EP, Francis J, Eades SC. Bronchial oxidative stress in equine recurrent airway obstruction.
This study was presented at the Experimental Biology meeting in New Orleans, April 21, 2009.
Venugopal CS, Mariappan N, Koch C, Holmes EP. Evidence for interaction between endothelin receptors in equine airways. This
study was presented at the Experimental Biology meeting in New Orleans, April 21, 2009.
Venugopal CS. Role of endothelin-1 in equine recurrent airway obstruction. This was presented at the 4th World equine airways
symposium in August 7th 2009 (Bern, Switzerland).
30
Dr. Frank Andrews:
Validation of the use of the SmartPill® Capsule for measurement of transit time, pH, temperature and luminal pressure in the
gastrointestinal tract of horses and dogs. SmartPill® Corporation, Buffalo, NY. 2007-2010. $21,000 (Transferred from UT).
An evaluation of the clinical efficacy of pergolide mesylate tablets for the control of clinical signs associated with pituitary pars
intermedia dysfunction (Equine Cushing’s Disease) in horses. Elanco Animal Health and Boehringer Ingelheim Vetmedica, Inc.
2008-2010. $214,358.
Extended use of pergolide mesylate in horses. Boehringer Ingelheim Vetmedica, Inc. 2009-2010. Up to $9,000.
Dr. Ann Chapman:
Chapman AM, Eades SC, Johnson JR. Identification of exposure to and shedding of Lawsonia intracellularis in Louisiana horses
using serology and real time PCR, EHSP Corp Fund, $12,000
Chapman AM. Identification of fecal shedding of Salmonella on a Virginia Thoroughbred breeding farm using real time PCR. VCS
Faculty start-up funds, $2,000
Dr. Shafiqul Chowdhury:
ELI LILLY. Improved Genetically Engineered Vaccines Against Bovine Herpesvirus Type 1 (BHV-1) and Equine Herpesvirus (EHV-
1) Type 1 Viruses. Total award $315,000 (12-20-2010- 12-19- 2012).
Dr. Susan Eades:
Eades SC, Fugler LA. Treatment of equine laminitis with Doxycycline. Grayson Foundation Special Barbaro Fund. $58,585.
2008-2010.
Eades SC, Fugler LA. The effects of oral administration of doxycycline on matrix metalloproteinase inhibition and experimentally-
induced laminitis. $25,000. Equine Health Studies Program, Louisiana State University School of Veterinary Medicine.
Fugler LA, Eades SC. Effects of LPS on matrix metalloproteinase release and biomechanical testing in laminar explants in vitro.
$6,000. Equine Health Studies Program, Louisiana State University School of Veterinary Medicine
McConnico R, Thompson J, Eades S, Paulsen D. Effects of clenbuterol on skeletal muscle composition, cardiac function and
biochemical markers of muscle injury in exercising horses. $12,500. Equine Health Studies Program, Louisiana State University
School of Veterinary Medicine
Venugopal C, Francis J, Eades S. Role of oxidative stress and endothelin in recurrent airway obstruction (RAO) in horses. $12,500.
Equine Health Studies Program, Louisiana State University School of Veterinary Medicine
Venugopal CS, Eades SC. Identification of a marker for equine summer pasture-associated obstructive pulmonary disease
(SPAOPD). Equine Health Studies Program. 2008-2009, $12,500.
Chapman A. Identification of Lawsonia Intracellularis in horses in Louisiana. LSU EHSP. $12,500.
2008-2010 GRANTS
31
2008-2010 GRANTS
Venugopal C. In vitro investigation of insulin resistance on equine digital vessels. LSU EHSP. $12,500.
Eades S, Andrews F. Validating a model to measure digital arterial pressure in the horse. ELANCO Animal Health. October 2010 to
December 2010. $25,312.
Eades S, Andrews F. The Pharmacokinetics and Vasoactive Properties of LSN2798286 (Amino-Tadalafil) in Mature Horses
ELANCO Animal Health. November 2010 to November 2011. $44,979.
Venugopal C, Francis J, Beadle R, Eades S. Role of oxidative stress and endothelin in recurrent airway obstruction (RAO) in
horses. EHSP 2010. $12,000.
Dr. Bruce Eilts:
A Canine Model For Nonsurgical Embryo Recovery, Embryo Cryopreservation, Embryo Culture and Nonsurgical Embryo Transfer
In Endangered Canids. $97,036
In vivo fertilization and culture of ungulate embryos in the bovine oviduct. ACRES. $50,000.
Dr. Jill Johnson:
Implementing a geographical information system (GIS) to investigate nosocomial Salmonella infections. Johnson JR, Eades SC,
Chapman A, Hosgood G. EHSP, $3,640.
Johnson JR. Capturing Salmonella from Biofilm in Water Sources, Pall Life Sciences provided water filters for screening water
sources in equine hospital for Salmonella organisms.
Chapman AM, Eades SC, Johnson JR. Detection of Lawsonia intracellularis in horses in Louisiana using serology and real-time
PCR. Equine Health Studies Program, $12,000.
Dr. Jose Len:
Len J, Beehan D. Effects on equine sperm cells of centrifugation with or without cushion solution. Funded by a $3,000 grant from
the EHSP.
Dr. Mandi Lopez:
Comparison of Two Soft Tissue Fixation Devices for Anterior Ligament Cruciate Reconstruction in a Canine Model. Tesa Medical,
Inc., $71,013. (4/2010 – 10/2010).
Lopez MJ, Gimble J. Equine bone regeneration with adult stem cells Grayson Jockey Club Foundation, $157,830. (4/2010
– 3/2012).
Lopez MJ. Travel Grant for Emerging Faculty. LA Board of Regents, $1,200. (8/2010).
Lopez MJ. Optimization and Characterization of Equine Adult Stem Cells for Bone Regeneration. LSU SVM USDA, $7,500.
(09/2009 – 08/2010).
32
Lopez MJ, Lin X, Zanetti A. Optimization and characterization of equine adult stem cells for bone regeneration. LSU Equine Health
Studies Program, $7,500. (7/2009-9/2010).
Lopez MJ, LRN Kaidapuram. Generation of a computer model of equine motion for therapeutic riding. LSU Equine Health Studies
Program, $6,000. (7/2009-6/2010)
Lopez MJ, Xie L. Comparison and quantification of equine adipose and marrow derived adult stem cell adhesion, viability,
proliferation, and induction behavior on three bioabsorbable scaffolds. LSU Equine Health Studies Program, $12,000.
(7/2009-6/20010)
Lopez MJ, Zanetti A. Osteogenic differentiation of equine adult stem cells (EASCs) in biodegradable scaffolds: a rat model for
equine bone reconstruction. LSU Equine Health Studies Program, $12,000. (7/2009-6/20010)
Lopez MJ. Travel Grant for Emerging Faculty. LA Board of Regents, $1,200. (6/2009)
Gao A., Lopez MJ. Post-doctoral training Fellowship People’s Republic of China $40,000. (6/22/2009-6/21/2010)
Lopez MJ, Gimble JM. Optimization of Adult Adipose Stem Cells for Bone Healing . LA EPSCoR (PFUND), $10,000.
(4/1/09-3/31/10)
Bagby G, Molina P, Shellito J (New Orleans Health Sciences Center, Lopez Core Training Faculty Member). T32: Biomedical
Alcohol Research Training Program (#2T32AA007577-11). NIH-NIAAA $1,952,500. (9/1/09-8/31/14)
Nelson S (PI, New Orleans Health Sciences Center. Lopez collaborator). Alcohol, HIV and Host Defense. NIH-NIAAA,
$10,000,000. (12/01/2009 – 11/30/2014)
Mao J (PI, Columbia University. Lopez subcontract). Tracking stem cells in engineered tissue and organs in vivo and in real time.
NIH - NIAMS, $126,765. (Sept. 2009 – Aug. 2014)
Feldman M, Hah D, Lopez MJ, Barton J. R21: Optical probe for microscopic cancer diagnosis in minute structures. NIH-NCI,
$411,327. (April 2009 - April 2011)
Lopez MJ. Demineralized Bone Matrix with Syngeneic Adult Adipose Multipotent Stromal Cells Accelerates Spinal Fusion
Compared to Demineralized Bone Matrix Alone in an Athymic Rat Model, Allosource, $39,248. (12/2010)
Lopez MJ. Intra-articular Filter-derived Platelet Therapy to Alleviate Signs of Equine Osteoarthritis. Pall Corporation, $30,000.
(6/2010)
Lopez MJ. Osteogenic differentiation of equine stem cells (EASCs) in biodegradable scaffolds: a rat model for equine bone
reconstruction. LSU Equine Health Studies Program, $7,500. (7/2010-6/2011)
Lopez MJ, Kaidapuram LRN. Generation of a computer model of equine motion to study therapeutic riding. LSU Equine Health
Studies Program, $3,300. (7/2010-6/2011).
2008-2010 GRANTS
33
Lopez MJ, Pinto V. Isolation and characterization of adult stromal cells from the equine laminae. LSU Equine Health Studies
Program, $8,800. (7/2010-6/2011)
Lopez MJ. Administrative supplement to adult stem cell suppression due to alcohol induced microenvironment alterations. NIH-
NIAAA, $47,040. (4/2010)
Lopez MJ. Comparison of two soft tissue fixation devices for anterior ligament cruciate reconstruction in a canine model. Tesa
Medical, Inc., $71,013. (4/2010)
Lopez MJ, Gimble J. Equine bone regeneration with adult stem cells Grayson Jockey Club Foundation, $157,830. (4/2010
– 3/2012)
Lopez MJ. Travel grant for emerging faculty. LA Board of Regents, $1,200. (2010).
Dr. Sara Lyle:
Beehan, D, Lyle, SK, Eilts BE, Len JJ, Love D. The investigation of the effects of lodixanol during equine semen chilling and
cryopreservation. EHSP Research Funds, $3234.
Len JA, Beehan D, Lyle SK, Eilts BE, Love C. Effect of a cushion solution during centrifugation on equine sperm cells. EHSP
Research Funds, $3558.
Dr. Rebecca McConnico:
McConnico RS, Mirza M, Wolfson W, Poirrier RA, Navarre C, Heidorn N, Miller C, Still R,
State/Regional Disaster Management Training Program grant award, American Veterinary Medical Foundation (AVMF), providing
emergency response training for veterinary students, veterinarians, first responders, and others to aid animals before, during, and
after disasters, $20,000 matched with in-kind gifts for a total value of $63,200, 2010, funded
McConnico RS, Scholarship award for college of veterinary medical faculty, The Chi Institute for Traditional Chinese Veterinary
Medicine, Reddick, Florida, $4,000, 2010, funded
McConnico RS, Teaching grant, LSU SVM professional curriculum and continuing education courses in Disaster Medicine and
Response, International Fund for Animal Welfare, $1,200, 2008, $3,000, 2010, funded
McConnico RS, Mirza M, Wolfson W, Poirrier RA, Teaching grant, LSU SVM professional curriculum and continuing education
courses in Disaster Medicine and Response, LSU’s Stephenson’s Disaster Management Institute, 2008, $2,500, 2009, $2,500,
2010, $1,000, funded
McConnico RS, Paulsen D. The effect of COX1-sparing agents on secretion in the equine right dorsal colon. LSU-Equine Health
Studies Program competitive grants program. $12,344, 2010
McConnico RS, Khodadad J. Examining the pharmacokinetics and safety of firocoxib, a COX-1 sparing NSAID in foals. Merial, Inc.
$13,562, 2009-2010
2008-2010 GRANTS
34
McConnico RS, Andrews F, Eades SC, Roy A, Littlefield MC. Consecutive equine herpes virus infection outbreaks at Louisiana
thoroughbred racing venues. LSU-Equine Health Studies Program, $3500, 2008-2009
Fernandez R, McConnico RS, Koch CE. Effects of Buscopan and flunixin on permeability and secretory capacity of equine right
dorsal colonic mucosa. Louisiana State University, School of Veterinary Medicine Merial Summer Scholars Program, Merial, Inc.,
$5,500, 2010
Khodadad J, McConnico RS. Examining the pharmacokinetics and safety of firocoxib, a COX-1 sparing NSAID in foals. Louisiana
State University, School of Veterinary Medicine Merial Summer Scholars Program, Merial, Inc., $5,500, 2009
Hayden M, McConnico RS, Barker S. Levamisole administration causes equid and bovid urine to test-positive for amphetamine,
Louisiana State University, School of Veterinary Medicine Merial Summer Scholars Program, Merial, Inc., $5,500, 2008
Hosgood, G, Snow L, Davidson J, McConnico RS. Effects of carprofen on canine gut barrier function. LSU SVM Veterinary Clinical
Sciences CORPS Funding, $3,500, 2008
Dr. Laura Riggs:
Evidence for generalized integumentary disruption in naturally-occurring laminitis” Equine Health Studies Program $12,000
Dr. Gary Sod:
Sod G, Durham M, Riggs L. Comparison of extraction torques between plasma sprayed hydroxyapatite coated and uncoated
4.5 mm AO cortical screws used to secure a 4.5mm broad dynamic compression plate (DCP) in equine third metacarpal bones,
$12,000, EHSP
Sod G, Baia P, Riggs L. Comparison of extraction torques between 5.5 mm AO cortical screws with roughened contact surface
and regular 5.5 mm AO cortical screws with polished contact surface used to secure a 4.5mm broad dynamic compression plate in
equine third metacarpal bones. $8,000.00, EHSP
Dr. C.S. Venugopal:
Pharmacological evaluation of potential drugs for equine laminitis: an in vitro study. PI: Venugopal CS. Co-investigators: Beadle RE
and Eades, SC. Agency: USDA Formula Funding, Amount: $12,000; Duration: Sept 1, 2010 to Aug 31, 2011. Received a “no cost
extension” for another year (Aug 31, 2012)
In vitro investigation of insulin resistance on equine digital vessels. PI: Venugopal CS. Co-investigators: Beadle RE and Eades SC.
Agency: Equine Health Studies Program, Amount: 12,000. Duration July 1, 2009 to June 30, 2010.
Identification of a biomarker for recurrent airway obstruction in horses. PI: Venugopal CS. Co-investigators: Eades SC and Beadle
RE. Agency: Equine Health Studies Program, Amount:$12,000. Duration: July 1, 2008 to June 30, 2009.
Role of oxidative stress and endothelin in recurrent airway obstruction in horses. PI: Venugopal CS. Co-investigators: Joseph
Francis and Eades, SC. Agency: Equine Health Studies Program, Amount: $12,000. Duration: July 1, 2007 to June 30, 2008.
2008-2010 GRANTS
35
Interaction of endothelin receptors in equine airways. PI: Venugopal CS. Co investigators: Joseph Francis and Mandi Lopez.
Agency: USDA Formula Funding. Amount: $10,000. Duration: Sep 15, 2007 to Sep 14, 2008.
2008-2010 GRANTS
36
State-of-the-Art Facilities and Equipment
The Equine Health Studies Program is an interdepartmental, multidisciplinary equine biomedical program within the LSU School of
Veterinary Medicine that is dedicated to the health, well-being and performance of horses through veterinary research, education,
and service. A diverse group of faculty, graduate students, post-doctoral fellows and staff conduct cutting-edge research in equine
health and disease. The program maintains a herd of approximately 90 horses and ponies for teaching and research. Three
research barns in the facility contain over 40 stalls and several pastures and paddocks are available for turnout and housing. Since
the last research report (2006-2007) we have upgraded and added some key facilities and equipment. These include the naming
of the EHSP physiology and pharmacology laboratory as the Charles V. Cusimano Equine Physiology & Pharmacology Laboratory,
updating of the Equine Performance Evaluation Laboratory and Lameness Pavilion, the Equine Cell & Tissue Culture Laboratory,
the Laboratory for Equine and Comparative Orthopedic Research, and the Equine Molecular Laboratory. Additionally, the clinical
facilities and equipment within the Veterinary Teaching Hospital and other core research facilities and resources within the School
of Veterinary Medicine support the research activities of the EHSP. The information highlights the updates and facilities since the
last research report.
Charles V. Cusimano Equine Physiology & Pharmacology Laboratory
Numerous faculty and graduate students conduct
scientific investigations involving equine physiology,
pharmacology, and pathophysiology, including, but not
limited to, vascular and nonvascular smooth muscle
physiology, pathology, and pharmacology (digital and
intestinal vasculature, bronchial, uterine and intestinal
smooth muscle), mucosal injury due to non-steroidal-anti-
inflammatory drugs, mucosal physiology and permeability,
endotoxemia, laminitis, and development and testing the
effects of medications on hemodynamics. We currently
have 15 Ussing’s chambers systems and multiple tissue
baths for measuring gastrointestinal mucosal permeability
and injury and muscular contraction.
Ussing’s chambers for gastrointestinal permeability studies
EHSP FACILITIES AND EQUIPMENT
37
EHSP FACILITIES AND EQUIPMENT
Tissue baths in the Charles V. Cusimano Physiology and Pharmacology Laboratory.
Laminitis Research Laboratory
Pfeiffer-Burt Lameness and Performance Pavilion
The Pfeiffer-Burt Lameness and Performance
Pavilion (75’ x 125’) is a modern facility for evaluation
of locomotion in horses. Several research projects
evaluating the efficacy of pharmaceutical and
nutraceutical agents and feed additives for treatment of
lameness in horses are being evaluated in this facility.
Lameness examination in the Pfeiffer-Burt Lameness Pavilion
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Force Plate Analysis in the Pfeiffer-Burt Lameness Pavilion
Equine Performance Evaluation Laboratory
The Equine Performance Evaluation Laboratory (EPEL) is equipped with a high-speed treadmill for exercising horses at speeds
that mimic racing conditions. A Coda Motion System digital motion analysis system that incorporates markers on the horse into
digital images to evaluate gait and lameness, is also available (see image below). Evaluation of the dynamics of the upper airway
can be achieved by a dynamic endoscopic system. We recently added a dynamic endoscope that can evaluate the upper airway in
research and clinic horses exercising on the high-speed treadmill or working in their natural environment, such as the racetrack or
riding arena.
High-speed treadmill with Coda Motion analysis system attached
EHSP FACILITIES AND EQUIPMENT
39
EHSP FACILITIES AND EQUIPMENT
Dynamic Endoscope
Laboratory for Equine and Comparative Orthopedic Research
The Laboratory for Equine and Comparative Orthopedic
Research was established and designed to facilitate a
strong association between clinical and basic orthopedic
research for advancement of orthopedic knowledge
across species and disciplines. The Laboratory for Equine
and Comparative Orthopedic Research is specifically
designed and equipped for translational orthopedic
research from the molecular/genetic level to the structural
level.
The laboratory is equipped with the most modern
equipment for molecular/genetic work, including an
MJ Research Chromo4 Detector and DNA Engine 200
for DNA fragment amplification and Quantitative PCR,
a UVP hybrilinker for blot analysis and a Synergy HT
multi-detection microplate reader for ELISA assays.
Housed within the laboratory is a Leica DM 4000 light
microscope with fluorescent, polarizing and phase
contrast capabilities. The microscope is equipped with the
latest in digital image capture equipment and software.
Additionally, there is a PathScan Enabler to obtain
ultra high quality images from 1 x 3 inch glass slides. A
custom-designed servohydraulic axial torsional Material
Testing System with a Flex Test SE Controller and equipped with a Multiple Gage Length Axial Extensometer makes nearly any
level of mechanical tissue testing possible from the tissue and bone level to joint and whole limb testing. Presently, several state-
40
of-the-art diamond saws are available for both orthopedic hard and soft tissue microscopic and ultrastructural sample preparation.
A section of the laboratory is devoted to histologic preparation of both calcified and decalcified tissue samples. Areas of research
focus include the pathophysiology of hip dysplasia, the development and implementation of novel orthopedic devices and cranial
cruciate ligament disease. Also, the laboratory is active in equine stem cell research, regenerative medicine and tissue culture.
The laboratory focuses on translation of basic scientific research to the live horse. Projects within the laboratory include growth of
laminar cells for use as an in vitro model of laminitis and bone marrow stem cells for use in tendon healing.
Equine Orthopedics and Biomechanics
We have developed a solid research program in the area of equine orthopedics and biomechanics. This has led to the
development of numerous orthopedic implants designed specifically for equine use, which is critical for the advancement of
equine orthopedics and fracture repair. These equine specific orthopedic implants have distinct advantages over those intended
for human application. The EHSP is unique in that it is the only school of veterinary medicine in the country that is designing and
testing equine specific orthopedic implants. An integral part of this research program was the development of a finite element
computer model that allows for the biomechanical testing of an orthopedic implant applied to an equine bone or bones. This allows
for changes in implant design to be made and tested using the finite element model prior to in vitro biomechanical testing. This
computer aided design approach allows for more efficient use of the limited cadaver specimens. This research has direct and
often immediate clinical applications. We are presently advising and performing biomechanical tests on prototype large animal
orthopedic implants for Synthes Vet, Inc., Innovative Animal Products, and IMEX Veterinary, Inc.
EHSP Clinical Facilities and Equipment
The Veterinary Teaching Hospital is staffed 24/7 with board certified and internationally recognized veterinary specialists (internal
medicine, surgery, anesthesiology, and radiology) and highly-skilled veterinary technicians that provide clinical service to the
horse-owning public. Since the last research report, the LSU Veterinary Teaching Hospital has added a new modern state-of-the-
art MRI unit that for evaluation of soft tissue and bony lesions in the head, neck, and limbs of horses with hard to diagnose clinical
conditions, including foot and lower limb lameness.
MRI Unit
The clinical facility has added new digital endoscopy equipment (Karl Storz, Inc.) to diagnose conditions of the upper and lower
airways, esophagus, stomach and reproductive tract.
EHSP FACILITIES AND EQUIPMENT
41
EHSP FACILITIES AND EQUIPMENT
Diagnostic and Research Endoscopy
Standing endoscopy of esophagus, stomach, and duodenum (see story ‘Researching Gastric Ulcer Disease” on page 60).
Equine Reproduction/Theriogenology Laboratory
The Equine Reproduction/Theriogenology Laboratory has
complete facilities for the evaluation, chill-transport, and
cryopreservation of spermatozoa, including light and phase-
contrast microscopes and a computer-assisted spermatozoal
analysis system (Spermvision®). We have three ultrasound
machines with 5-7.5 MHz linear array transducers and
a 5-7.5 MHz sector array transvaginal transducer for
oocyte collection by follicular aspiration or twin reduction
by aspiration. A fourth ultrasound system equipped with
a 3.5 sector and 5-7.5 microconvex array is available for
transabdominal imaging. An Olympus endoscope is also
available for hysteroscopic examination and for hysteroscopic
low-dose insemination. Laparoscopy is available and used for
oviductal insemination and for minimally invasive placement
of intrauterine catheters. We maintain a close collaborative
relationship with the Equine Biotechnology Laboratory,
which is part of the LSU Agricultural Center. This facility has
tissue culture laboratories and micromanipulators that make
possible such advanced assisted reproductive techniques as
intracytoplasmic spermatozoal injection and nuclear transfer
(“cloning”).
42
Equine Molecular Biology Research Laboratory
The Equine Molecular Biology Research Laboratory is equipped to support the molecular biology aspects of research conducted
by the EHSP investigators. The missions of this laboratory are to perform basic, cutting-edge research to explain the molecular
basis of disease with a view to improved clinical approaches; to train scientists, students, and visitors at all levels; and to develop
new instruments and methods in equine molecular biology. We also have direct access to the core facilities within the Division of
Biotechnology and Molecular Medicine (BIOMMED) within the LSU School of Veterinary Medicine for quantitative Real-Time PCR,
primer/probe design, Quantity One for DNA fragment visualization and analysis, SDS-PAGE analysis, MagnaPure automated
nucleic acid extraction, and microarray spotters and readers. Current investigations utilizing this laboratory include study of key
mediators in equine laminitis, metabolic disease, gastrointestinal disease, summer pasture-associated recurrent obstructive airway
disease, bone healing and mechanisms of pain sensation and modulation. The capabilities of this laboratory have expanded in light
of the increasing importance of the molecular biological approach to the investigation of equine health and disease.
EHSP FACILITIES AND EQUIPMENT
43
Disaster Preparedness
LSU Veterinary School associate professor speaks at World Congress on Disaster and Emergency Medicine
The 17th World Congress on Disaster and Emergency
Medicine (WCDEM) closed on June 3. Held in Beijing,
China, the Congress hosted more than 1,600 participants
from 57 countries.
The 17th WCDEM was held in conjunction with the 14th
Annual Meeting of the Chinese Society for Emergency
Medicine. With the scale and frequency of disasters
increasing, the 17th WCDEM was more relevant than
ever. This year alone marks one of the worst in terms of
disasters—the earthquake, tsunami, and nuclear reactor
issues in Japan; floods in Australia; earthquakes in New
Zealand; and floods and tornados in the United States.
Research, experiences, and lessons learned from these
recent events, and many others, were presented by
delegates at the 17th WCDEM.
LSU School of Veterinary Medicine’s expanding disaster
training program was highlighted with two presentations
by Rebecca S. McConnico, DVM, PhD, DACVIM (LSU
SVM 1987), associate professor of veterinary medicine
in the Department of Veterinary Clinical Sciences. Dr.
McConnico’s presentations were added as part of a
special veterinary component of this international meeting
due in part to expanding global concerns about societal
responsibilities for including animals in local, regional, and
federal disaster planning. Dr. McConnico’s presentation topics were biosecurity considerations for equine emergency sheltering
and experiential learning in disaster response for veterinary students and veterinarians.
RESEARCH NEWS & DEVELOPMENT
TOP: Dr. Rebecca McConnico, associate professor of veterinary medicine at LSU’s School of Veterinary Medicine, and Dr. Dick Green, international director of emergency response, International Fund for Animal Welfare, in front of the conference banner in Beijing, China.
BOTTOM: Attending the World Congress on Disaster and Emergency Medicine are (from left) Dr. John Madigan, Medicine, professor of equine medicine, University of California Davis, School of Veterinary; Dr. Heather Case, director of the American Veterinary Medical Association’s Scientific Activities Division and the Louisiana State Animal Response Team coordinator of emergency preparedness and response; Dr. Rebecca McConnico, associate professor of veterinary medicine at LSU’s School of Veterinary Medicine; and Dr. Diane McClure, associate professor, Western University of Health Sciences.
44
LSU Veterinary School dedicates laboratory in honor of Charles V. Cusimano
The LSU School of Veterinary Medicine recently dedicated the Equine Physiology and Pharmacology Laboratory in honor of
Charles V. Cusimano. “Our purpose in recognizing Mr. Cusimano is to say, ‘Thank you’ for his enduring support and valuable
efforts to the school and the Equine Health Studies Program,” said Frank Andrews, DVM, PhD, director of the EHSP.
Mr. Cusimano’s family and friends, as well as many LSU faculty and staff, gathered at the LSU SVM on March 3 for the dedication
ceremony. Dean Peter F. Haynes served as master of ceremonies. “Today is all about Charles V. Cusimano, his passion and
support for LSU and the School of Veterinary Medicine, and his central and pivotal role in establishing our Equine Health Studies
Program,” said Dean Haynes.
Representing the LSU System was President John Lombardi, and representing the LSU Baton Rouge campus was Chancellor
Mike Martin, who said, “Great universities are known by the company they keep, and it’s great to keep the kind of company that
the Cusimanos bring to this campus. It sends a message to those folks out there that this is a first-rate place because we deal with
first-rate people.”
Dr. Mike Strain (LSU SVM 1983), Louisiana Commissioner of Agriculture & Forestry, told the crowd about working with Mr.
Cusimano to secure dedicated funds for the EHSP from slot machine proceeds from Louisiana’s racetracks. “It took seven years to
make this work,” said Dr. Strain. “One year we got the bill going, but it was bogging down. In 2003, Charlie comes to me and says,
‘We’re going to see Mike [Foster].’ We went to see the governor and got his support, but the bill kept getting referred to different
committees. On the last day, at the last hour, we got the bill through and began a research program that has paid huge, huge
dividends.”
Charles Vincent Cusimano, a native of New Orleans, Louisiana, graduated from Louisiana State University with a BS in petroleum
Photo: From left, Dean Peter F.
Haynes and Commissioner of
Agriculture & Forestry Mike Strain
(LSU SVM 1983) congratulate
Charles Cusimano during
the dedication of the Equine
Physiology and Pharmacology
Laboratory. Mr. Cusimano was
joined on stage by his wife, Violet
Cusimano.
EHSP FACILITIES AND EQUIPMENT
45
engineering in 1951. He attended LSU on a football scholarship, lettered for four years, and played in the Cotton Bowl in 1946 and
the Sugar Bowl in 1950. Cusimano also studied law at Loyola University in New Orleans and did postgraduate work in geology at
the University of Oklahoma.
Cusimano is a former member of the Louisiana State University Board of Supervisors who began his service in 1974 and served as
chairman in 1995-1996. He was admitted to the LSU Law Hall of Fame in 1987 and the Engineering Hall of Distinction in 1999. In
addition, he helped establish religious centers on all of LSU’s campuses.
Photo: Charles Cusimano cuts the
ribbon to the Equine Physiology
and Pharmacology Laboratory,
which has been dedicated in his
honor, alongside his wife, Violet
Cusimano, and LSU System
President John Lombardi.
EHSP FACILITIES AND EQUIPMENT
46
How You Can Support the EHSP and Enhance the Health, Well-Being and Performance of Horses
There are many ways individuals, foundations and companies can help support the LSU School of Veterinary Medicine’s Equine
Health Studies Program. The EHSP is “dedicated to the health, well-being and performance of horses through veterinary research,
education and service.” In order to fulfill our mission of becoming one of the premier equine biomedical centers in the country, we
have initiated a campaign to generate funds to enhance all aspects of our program.
Our endowment is comparatively small, so each gift is extremely special to us and will make an important and immediate impact on
our programs. Our fundraising efforts have been principally through private, charitable, tax-deductible gifts, as well as some other
special events and activities. All gifts are tax-deductible and can be pledged with a portion given annually over a period of a few
years. We hope that you will consider assisting us with our fundraising efforts for facility enhancements, endowed/distinguished
professorships and chairs, and/or scientific advances.
An endowed gift is a permanent gift. The principal is invested and returns annual interest. Part of the annual interest is reinvested
to increase the principal, and part is used for the intended purpose (such as a professorship/chair or research). Endowed funds are
usually named for the benefactor or for a designated honoree.
Professorships and Chairs
The state of Louisiana has a matching program for Endowed Professorships and Endowed Chairs. The School currently has
only one Endowed Chairs and three Endowed Professorships, none of which are in the area of equine health and performance.
Endowed positions are vital to our instructional and investigational programs. The individuals in these positions will serve as
leaders of teams of equine clinicians and investigators that conduct cutting-edge scientific investigations.
Equine Biomedical Research
Private gifts can provide funds for leading-edge science specific to the horse. With the limited amount of state and federal funding
available for equine scientific investigations, funds from private, charitable gifts are critical to improve successful prevention and
treatment of illnesses and injuries that can be performance-limiting, career-ending and even life-threatening.
General EHSP Support Fund
Gifts can be made to the General EHSP Support Fund (a non-endowed account). These funds may be used to purchase
equipment for equine scientific investigations. Additionally, they are used for continuing education for the horse-owning public and
private equine veterinarians. These funds also are used to assist with other educational, promotional and fundraising activities.
Equine Farrier Unit
Gifts can be made to the Pfeiffer-Burt Lameness and Farrier Unit. These funds will be used to supplement current funding to
provide equipment for diagnosis and treatment of medical and surgical conditions of the equine hoof.
Equine Reproduction
Gifts can be made to the EHSP Equine Reproductive Center. These funds will be used to construct a state-of-the-art equine
reproductive center that will provide diagnosis and treatment of horses with medical or surgical problems that lead to infertility or
decreased conception rates.
EHSP DEVELOPMENT
47
EHSP DEVELOPMENT
Memorial Gifts and Naming Opportunities
Your gift may be used to honor or memorialize a beloved horse, family member, or friend. Naming opportunities exist for endowed
gifts like scholarships, professorships and chairs. Newly constructed facilities and novel projects offer a wide variety of naming
opportunities, including the Equine Intensive Care Unit and the Equine Reproduction Unit.
Again, any gift will be very special to the LSU School of Veterinary Medicine’s Equine Health Studies Program and will make a
dramatic and immediate impact on our teaching, service and scientific investigation programs. We thank you for your generosity
and support. To learn more about how your gift will assist the EHSP with its mission, please visit our website (www.equine.vetmed.
lsu.edu) or contact Ky Mortensen via telephone (225-578-9590) or e-mail ([email protected]).
I want to support the LSU SVM EHSP!Please use my gift to continue the excellence in community service, education, and research through the EHSP.
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48
BIOMECHANICS AND ORTHOPEDICS
BIOMECHANICAL ALGORITHM TO QUANTIFY FORCES FROM EQUINE MOTION AT THE SADDLE POSITION
Authors/Investigators
Laxmi R Nandana Kaidapuram, BTech,MS; Mandi J Lopez, DVM, MS; Lin Xie, BS
Description of the Problem
There is no scientific method or algorithm in literature to determine the most appropriate horse for each individual based on
functional parameters of the horse and the rider in therapeutic riding.
Study Purpose/Objectives
Therapeutic horseback riding is a common component of many physical therapy programs. Forces transferred to the rider vary with
horse size, breed and gait. Interaction between a horse and rider is a complex problem of coupled dynamic body interactions. The
potential forces transferred to the rider may be predicted by quantification of the forces on to a static weight from the horse back at
the level of the saddle. To accomplish this task, an algorithm to quantify forces at the saddle level from ground reaction forces was
developed using a combination of direct and indirect dynamic analysis to test the hypothesis that the forces transferred to a static
weight at the saddle level on can be predicted by the breed and weight of the horse.
Approach
Seven adult horses were used for data collection: 3 Thoroughbreds, 3 Quarter Horses and 1 Paso Fino (4 geldings and 3 mares),
5-14 years; 387-580 kg. Simultaneous, real time kinetic, kinematic and back force data on a static weight was collected at a
walk using an integrated system consisting of a force platform, active motion detection system, and wireless force transducers,
respectively. Infrared light emitting markers were glued to the skin at 22 specific anatomical landmarks. A customized surcingle
fixture to hold a 12kg weight on top of wireless force transducers were used for data collection at the saddle level. Kinematic data
and ground reaction forces (GRF) were collected. Forces at the saddle level were measured with a wireless load force system
which was synchronized with the force platform and motion capture system using a double mouse. Data was collected from a
minimum of four trials from all horses at a walk (1.3-2.0 m/s). Inverse dynamic analysis was used to calculate the fore and hind limb
joint forces to the level of the shoulder and hip. Virtual segments were created from the shoulder to the spine, and the segments
were modeled as series of springs and dampers. The energy absorption coefficients were derived through a series of iterations to
align the inverse dynamic solutions of the fore- and hind limb data with the forces measured at the back.
Results/Conclusions
Forces at the saddle level on to a 12 kg. weight are shown in Figure 1 for three breeds of horses. For all the horses, the first peak
value occurs within the first 25- 50% of the stride cycle. Though the overall pattern of the force is same for all the horses, there is
significant variation in peak and valley values from breed to breed and horse to horse. Peak-to-peak force on the 12 kg. weight
is proportionated to 60 kg., which is equivalent to an average rider weight. Paso Finos produce forces at the saddle level that are
115% more than Thoroughbreds and 45% more than Quarter Horses. Forces at the saddle level are based on breed of the horse,
and it doesn’t show any significant variation with weight of the horse. In contrast, ground reaction forces depend on the weight of
the horse irrespective of the breed.
49
BIOMECHANICS AND ORTHOPEDICS
Acknowledgements
This study was supported by the EHSP.
Year Completed
2011
Published Manuscripts/Abstracts
Laxmi R Nandana Kaidapuram,BTech,MS; Mandi J Lopez,DVM,MS; Lin Xie, BS, Biomechanical algorithm to quantify forces from
equine motion at the saddle position. A poster was presented at the 2011 meeting of the American College of Veterinary Surgeons.
Forces at the saddle level on a 12 kg. weight at the rider level of Thoroughbreds, Quarter Horses, and Paso Fino.
Thoroughbred Horses
Quarter Horses
Paso Fino
50
An in vitro biomechanical comparison between dynamic compression plates having a roughened contact surface and dynamic compression plates having a polished contact surface for the fixation of osteotomized equine third metacarpal bones
Authors/Investigators:
Gary A. Sod, DVM, PhD, Laura M. Riggs, DVM, PhD, Diplomate ACVS, Colin F Mitchell, BVMS, MS, Diplomate, ACVS, George S.
Martin, DVM, MS, MBA, Diplomate, ACVS, Marjorie S. Gill, DVM, MS, Diplomate, ABVP
Year Completed:
2010
Published Manuscripts/Abstracts:
Sod GA, Riggs, LM, Mitchell CF, Martin GS, Gill MS. An in vitro biomechanical comparison between dynamic compression
plates having a roughened contact surface and dynamic compression plates having a polished contact surface for the fixation of
osteotomized equine third metacarpal bones. Veterinary Surgery, 40(4), 2011.
Sod GA, Riggs LM, Mitchell CF, Martin GS, Gill MS. Why do orthopedic implants fail in equine long bone fracture patients? - Part I
and Part II. Veterinary Orthopedic Society 35th Annual Conference, Big Sky, MT, March 2008.
A mechanical comparison of equine proximal interphalangeal joint arthrodesis techniques: an axial locking compression plate and two abaxial transarticular cortical screws versus an axial dynamic compression plate and two abaxial transarticular cortical screws
Authors/Investigators:
Gary A. Sod, DVM, PhD, Laura M. Riggs, DVM, PhD, Diplomate ACVS, Colin F. Mitchell, BVMS, MS, Diplomate ACVS, George S.
Martin, DVM, MS, MBA, Diplomate, ACVS
Year Completed:
2010
BIOMECHANICS AND ORTHOPEDICS
51
Published Manuscripts/Abstracts:
Sod GA, Riggs, LM, Mitchell CF, Martin GS. A biomechanical comparison of equine proximal interphalangeal joint arthrodesis
techniques: an axial locking compression plate and two abaxial transarticular cortical screws versus an axial dynamic compression
plate and two abaxial transarticular cortical screws. Veterinary Surgery, 40(4), -, 2011.
Sod GA, Riggs, LM, Mitchell CF, Martin GS. A biomechanical comparison of equine proximal interphalangeal joint arthrodesis
techniques: an axial locking compression plate and two abaxial transarticular cortical screws versus an axial dynamic compression
plate and two abaxial transarticular cortical screws. Proceedings of the World Veterinary Orthopedic Congress/Veterinary
Orthopedic Society 37th Annual Conference, Breckenridge, CO, February, 2010.
An In Vitro Cyclic Fatigue Comparison of a Modified 5.5 mm Locking Compression Plate Fixation with a 5.5 mm Locking Compression Plate Fixation of Osteotomized Equine Third Metacarpal Bones
Authors/Investigators:
Gary A. Sod, DVM, PhD, Laura M. Riggs, DVM, PhD, Diplomate ACVS, Colin F Mitchell, BVMS, MS, Diplomate, ACVS, George S.
Martin, DVM, MS, MBA, Diplomate, ACVS, Marjorie S. Gill, DVM, MS, Diplomate, ABVP
Year Completed:
2009
Published Manuscripts/Abstracts:
Sod GA, Riggs, LM, Mitchell CF, Martin GS, Gill MS. In vitro cyclic fatigue comparison of a modified 5.5 mm locking compression
plate fixation with a 5.5 mm locking compression plate fixation of osteotomized equine third metacarpal bones. Veterinary Surgery,
39(7), 833-838, 2010.
BIOMECHANICS AND ORTHOPEDICS
52
In Vitro Tissue Generation by Adult Equine Multipotent Stromal Cells on Collagen Scaffolds
Authors/Investigators
Lin Xie, BS; Anna Marsano, PhD; Yanru Zhang, PhD; Jeffrey Gimble, MD, PhD; Gordana Vunjak-Novakovic, PhD; Mandi J. Lopez,
DVM, MS, PhD
Description of the Problem
Adult equine multipotent stromal cell (MSC) research supports the potential of the cells to restore normal tissue structure and
function when combined with biocompatible scaffolds. Therefore, there is great potential to accelerate tissue repair with equine
stromal cell therapy in combination with other well-established medical and surgical techniques. To derive the greatest benefits
from equine stromal cell applications and propel the technology into clinical trials, it is necessary to optimize equine MSC-
scaffold constructs in vitro. Adult equine adipose- (ASC) and bone marrow-(BMSC) derived multipotent stromal cell adipogenesis,
osteogenesis and chondrogenesis on commercially available bovine collagen I (COLI) scaffolds were quantified in this study.
Study Purpose/Objectives
The overall goal of this study was optimization of adult equine multipotent stromal cell interactions with biocompatible scaffolds for
purposes of advancing clinical application of equine tissue regeneration technology. Several hypotheses were tested to support
this goal. The first hypothesis was that equine BMSCs and ASCs have indistinguishable cell seeding, attachment, viability, and
distribution on COLI scaffolds. The second, two-part hypothesis was that BMSC – COLI scaffold constructs are superior to ASC –
COLI scaffold constructs for osteogenesis and chondrogenesis while ASC – COLI scaffold constructs demonstrate higher superior
adipogenesis.
Approach
Cells in this study were isolated from 4 adult Thoroughbred horses from 7 to 10 years old. Bone marrow (BM) was collected
from sternebrae and adipose tissue was collected from superficial gluteal fascia. The collection procedure was approved by the
Institutional Animal Care and Use Committee. The cell surface marker profile of loaded cells was established just prior to loading
by flow cytometry. Adult equine BMSCs and ASCs were loaded into COLI scaffolds (10x7x3mm) at a loading density of 106 cells/
ml by perfusion bioreactors (Figure 1). Scaffolds were equally divided into sections which were subsequently cultured for 7, 14 or
21 days in stromal or adipogenic, osteogenic, or chondrogenic medium in triplicate. The mRNA levels of tissue specific genes of
cell-scaffold constructs were quantified by RT-PCR. Cell-scaffold constructs were also assessed for cell distribution and viability,
cell number, microstructure, and ultrastructure by confocal laser microscopy, DNA quantification, light microscopy, and scanning
electron microscopy, respectively. All values are reported as mean ± standard error (SE). Student’s t test was performed to
compare the effects of cell type on cell loading efficiency. Three-way ANOVA was used to compare the effects of cell type, post-
loading culture time and induction medium on the cell viability. Different combination effects were evaluated with Tukey’s post-hoc
tests. Significance was considered at P<0.05.
Results/Conclusions
Loading efficiency was 74.6% ± 14.4% and 85.8% ± 9.2% for BMSCs and ASCs, respectively. Both cell types were positive for
CD29, CD44, and CD105, and BMSCs were additionally positive for CD73 and CD90. Cells were uniformly distributed in the
scaffold immediately after loading. The mRNA levels of collagen I and sex determining Region Y-box 9 (Sox9) were significantly
STEM CELL AND REGENERATIVE MEDICINE
53
STEM CELL AND REGENERATIVE MEDICINE
higher in BMSC constructs while lipoprotein lipase (LPL) mRNA levels were significantly higher in ASC constructs at all time points
(Figure 2). Tissue specific micro- and ultrastructural changes were evident in cell-scaffold constructs cultured in induction versus
stromal medium (Figure 3).
Benefits to/Impact on the Equine Industry
This study evaluated the in vitro behavior of two types of adult equine MSCs on COLI scaffolds. Both cell types had high
viability and proliferation, confirming the suitability of COLI scaffolds for cell growth. Furthermore, COLI scaffolds supported cell
differentiation into distinct mesenchymal tissues. This systematic and comprehensive in vitro evaluation of interactions between
COLI scaffolds and equine MSCs proved their potential to support diverse mesenchymal tissue generation for equine industry.
Take Home Message
The versatility and suitability of type I collagen scaffolds for equine adult MSC tissue regeneration was confirmed by parallel
generation of distinct mesenchymal tissues by paired ASCs and BMSCs.
Acknowledgements
Funding for this project was provided by the LSU Equine Health Studies Program.
Year Completed
2010–2011
54
ISOLATION AND CHARACTERIZATION OF EQUINE LAMINAR PROGENITOR CELLS
Authors/Investigators
Vanessa Marigo, DVM; Marilyn A. Dietrich, BS, MSc; Frank Andrews, DVM, MS; Jeffrey M. Gimble, MD, PhD; Mandi J. Lopez,
DVM, MS, PhD, DACVS
Description of the Problem
The laminar tissue is a very unique structure that balances forces within the equine hoof. It has two major components, the dermal
component, attached to the coffin bone, and the epidermal component, attached to the hoof wall, preventing the distal phalanx
from rotating in response to tension from the deep digital flexor tendon. Failure of this complex relationship results in laminitis. This
disease affects about 15 % of horses in the United States each year, and 75 % of those affected, more than 8,000, lose their lives.
Costs associated with this devastating condition exceed $10 million per year for diagnosis, treatment, and lost revenues. Laminitis
is a multifactorial disease making identification of specific causes and treatment difficult. Knowledge of equine laminar cells
physiology will contribute to understand, prevent or reverse the damage caused by laminitis.
Study Purpose/Objectives
The objective of this project was to isolate primordial cells from equine laminar tissue characterize the proliferation rate, identify cell
surface markers expression and estrogenic differentiation potential in vitro.
Approach
Cells were isolated from laminar tissue from horses euthanized for reasons other than the present research. The in vitro expansion
characteristics of cells were determined with cell doubling number (CD) and doubling time (DT) assays. Limit dilution assays
were used to quantify colony forming units for fibroblasts (CFU-F). Cells from passages 1 and 3 cultured in stromal medium were
submitted to flow cytometry of fluorescein isothiocyanate (FITC) and phicoerithrin (PE) labeled cells to identify CD44, CD29 and
CD105, which are progenitor cells surface markers. To address the osteogenic potential cells isolated from the laminar tissue
were maintained in stromal media until 70% confluence, when media was changed to osteogenic induction media (DMEM-Ham’s
F12, 10% FBS, 1% antibiotic/antimycotic solution, β-glycerophosphate (10 µmol/L), dexamethasone (20 nmol/L), and sodium
2-phosphate ascorbate (50µg/mL). Cells were then cultured for 21 more days and media were. Cells were rinsed with 150mM NaCl
3 times and fixed in 70% ETOH. After fixation, cells were stained with a 2% Alizarin Red solution in distilled water.
Results/Conclusions
Cells had similar proliferation rates from passages 1 to 6. The overall mean ± SEM CD for P1 was 1.14±0.26 and P6 was
1.26±0.16 and DT for P1 was 2.30±0.57 and P6 was 5.19±1.14 days. The cells do not double as efficiently in later passages
compared to earlier passages, however this difference is not statistically significant. Primary fibroblastic phenotype (CFU-F)
occurred at a frequency of 1 per 552 nucleated cells, 1 per 302 nucleated cells and 1 per 457 for passages 1, 3 and 6 respectively.
Cells from passage 1 and 3 were had more than 70% positive staining for CD44, CD29 and CD105 suggesting these cells are
progenitor cells. After osteogenic induction cells then formed round colonies that stained with alizarin red indicating calcification.
LAMINITIS
55
LAMINITIS
Take Home Message
Our study showed that the cells harvested from equine laminar tissue have a constant proliferation rate in vitro until passage 6,
express specific cell surface markers and can be differentiated into osteoblasts. The significance of this project is that isolation and
characterization of adult stromal cells from the equine laminae will provide a new target area for research efforts to prevent and
cure the disease. The findings have significant potential to enhance understanding and treatment of equine laminitis. Moreover, it
can be a new model to investigate laminitis in vitro.
Acknowledgements
This study was supported by LSU Equine Health Studies Program.
Year Completed
2011
Published Manuscripts/Abstracts
Marigo, V., DVM, Gimble, J.M., MD, PhD; Andrews, F., DVM, MS; Lopez, M.J., DVM, MS, PhD, DACVS. Characterization of
Stromal Cells from Equine Laminae in vitro. Abstract at ACVS Veterinary Symposium, 2011.
Figure 1. Cell Doublings and Doubling Times. All values reflect one mean ± standard deviation.
Figure 2. Flow Cytometry and Osteogenesis. Representative graph of cells stained with CD44 (left) and alizarin red staining calcium deposition after osteogenic induction (right).
56
LAMINITIS
Treatment of Experimental Equine Laminitis with Doxycycline
Authors/Investigators
Susan Eades and Lee Ann Fugler
Matrix metalloproteinases (MMPs) are enzymes present normally in connective tissue including the laminar tissue that holds the
coffin bone into the hoof capsule. These enzymes breakdown proteins in collagen to remove connective tissue during normal
processes of hoof growth and repair. However, excessive matrix metalloproteinase activity is thought to lead to excessive
breakdown of laminar tissue allowing deviation of the coffin during laminitis. Oxytetracycline is an antibiotic that has other
properties in addition to its ability to kill bacteria. Oxytetracycline is also capable of inhibition of MMP enzymes allowing it to be
used in people with severe gingival disease and other disease where excessive collagen breakdown occurs. As a result the partial
success associated with use of oxytetracycline in people, practitioners have employed doxycycline for use in horses with laminitis.
The purpose of this study was to determine the effectiveness of doxycycline, oxytetracycline, and pentoxifylline (PTX) in the
treatment of equine laminitis using a corn starch overload (CSO) model.
The administration of PTX significantly decreased the severity of lameness
associated with laminitis (Fig 1). Doxycycline and oxytetracycline administration did
not alter the severity of laminitis caused by corn starch. Pentoxifylline reduced MMP
production; however, results of these studies also suggest that PTX also decrease
laminitis pain by reducing inflammation.
Figure 1. Lameness grades associated with laminitis in untreated (control) horses and horses treated with pentoxifylline (PTX)
Pentoxifylline is a medication used in people to reduce some of the
symptoms of muscle cramping and pain associated with arterial diseases.
This medication has also demonstrated the ability to reduce scar tissue
formation in chronic diseases of the kidney and lungs. Pentoxifylline
decreased the severity of lameness associated with laminitis induced by
corn starch. The therapeutic effects of PTX in CHO may involve MMP
inhibition and antiinflammatory effects. Pentoxifylline can be used by
veterinarians to reduce the severity of laminitis associated with intestinal
diseases and metritis.
57
GASTROINTESTINAL
Pharmacokinetics and clinical safety of oral paste firocoxib, a COX-1 sparing NSAID, in foals
Authors/Investigators
McConnico RS, Khodadad JL, Koch CE
This study evaluated pharmacokinetics and clinical safety of an oral paste formulation of a commercially available COX1-sparing
NSAID in clinically healthy pony foals in a randomized controlled clinical trial. Values for complete blood count, serum chemistry
profile, urinalysis, pharmacokinetic assay, and gastric endoscopy were evaluated in eighteen Shetland pony foals treated with
firocoxib (0.1 mg/kg, PO, q 24 h) or placebo for 14 days. Foals were divided into 3 treatment groups. Group 1 and 2 foals received
firocoxib while a 3rd group was administered an oral placebo. Gastric endoscopy was performed on group 1 and 3 foals prior to
treatment and on days 7 and 14 to monitor for the presence of gastric ulcers. Group 2 and 3 foals had blood and urine samples
taken sequentially for pharmacokinetic analysis, CBC, serum chemistry evaluation, and urinalysis. Physical examinations were
performed prior to treatment and daily for 17 days. Data were analyzed using anova and paired t-tests (P<0.05). None of the foals
presented adverse clinical effects. There were no significant changes in CBC, biochemical profiles within groups, or differences
between groups. Pretreatment gastric endoscopy scores were not significantly different from evaluations at 7 and 14 days.
Firocoxib was quickly absorbed with an observed maximum concentration at 2 hr, the first sampling interval, for the majority of
animals. Firocoxib plasma concentrations decreased in a log-linear manner after reaching the maximum concentration and steady
state concentrations were achieved by the 7th dose. Based on the sampling times after the final and 14th dose, an average half
life of 1.3 days was estimated. Administration of firocoxib did not cause any adverse effects on gastrointestinal, or hematological or
serum biochemical variables, appears to have been well tolerated, and follows a predictable pharmacokinetic pattern in 4-6 week
old foals.
Right Colonic Pathophysiology in Horses Administered Phenylbutazone
Authors/Investigators
Rebecca S. McConnico, DVM, PhD; Timothy W. Morgan, DVM, PhD; Cathleen C. Williams, MS, PhD; Jeremy D. Hubert, BVSc,
MRCVS; Rustin M. Moore, DVM, PhD
Study Purpose/Objectives
To determine pathophysiologic effects of phenylbutazone (PBZ) on equine right dorsal colon (RDC).
Approach
Animals—12 healthy adult horses.
Procedures—A controlled cross-over observational study was conducted. Clinical and serum variables, colonic inflammation
(histologic grading) and measurement of myeloperoxidase [MPO] activity, malondialdehyde [MDA] concentrations, and
prostaglandin E2 [PGE2] concentrations), ingesta volatile fatty acid (VFA), and arterial blood flow in the RDC were evaluated for a
58
GASTROINTESTINAL
21-day period in horses administered PBZ (8.8 mg/kg, PO, q 24 h) or a control substance.
Results—Data from 8 horses were used for analysis. Plasma albumin concentrations decreased significantly from days 10 to 21
during PBZ treatment, compared to during the same days for control treatment. Phenylbutazone treatment caused neutropenia (<
3.0 x 103 cells/αL). No other clinical or hematologic abnormalities were detected between PBZ or control treatments. Two horses
developed colitis while on PBZ. No significant differences were detected in the RDC between PBZ and control treatments for MPO
activity, MDA and PGE2 concentrations, or for histologic evidence of inflammation. Arterial blood flow in the RDC was significantly
increased during PBZ treatment, compared with values for controls. Differences were identified in VFA production during PBZ
treatment, compared with control treatment, with a decrease in acetic acid concentrations over time.
Results/Conclusions
Prolonged phenylbutazone administration caused hypoalbuminemia, neutropenia, changes in RDC arterial blood flow, and
changes in VFA production. Equine veterinarians should monitor serum albumin concentrations and neutrophil counts and be
extremely cautious when making dosing recommendations for phenylbutazone treatment.
Published Manuscripts/Abstracts
Am J Vet Res. 69(11):1496-505, 2009.
Acknowledgements
Support for this project was provided by the Grayson Jockey Club Research Foundation Incorporated.
New Approaches in Treating Equine Gastric Ulcer Syndrome
Authors/Investigators
Dr. Frank Andrews, Dr. Michelle Woodward, Dr. Patrick Loftin, Dr. Joshua
Cartmill, Dr. Nan Huff, Michael L. Keowen, Frank Garza, Jr.
EGUS is a common problem in horses. All ages and breeds are
susceptible, but the prevalence is especially high in Thoroughbred race
horses, probably because of the intense exercise they undergo during
training and racing. It is estimated that 60% to 90% of performance horses
(between 500,000 and 700,000 animals in the U.S. alone) are affected by
EGUS, which translates to millions of dollars of lost revenue each year in
training and racing days and in the cost of treatment. In addition, EGUS
can be a painful condition, causing considerable suffering among affected
horses and foals.
59
EGUS is a complicated syndrome with a number of potential causes, but risk factors include stress, being transported, diet,
stall confinement, intermittent feeding or fasting, intense exercise, racing, and the use of non-steroidal anti-inflammatory drugs
(NSAIDs). Clinical signs of EGUS, which are present in about half of the horses diagnosed with EGUS, tend to be vague and
include abdominal pain or colic, diarrhea, rough hair coat, poor appetite, depression, and decreased performance. It is poor
performance that makes EGUS an economically significant condition in the racehorse industry,
There are a number of commercial antiulcer drugs that can be used to treat EGUS, such as equine formulations of omeprazole
and ranitidine (both of which are also used in human medicine). But these drugs are expensive—often costing $40 or more per
day—and must be used for at least 28 days for complete healing. This can cause a financial strain for horse owners, who may not
be able to afford to treat their animals for the prescribed length of time, or may use the drugs at sub-therapeutic doses, or even
substitute other medications or feed supplements that are ineffective. Hence, the prevalence of EGUS remains high even with
effective treatment options available.
Dr. Andrews has been looking for a more cost-effective means of managing EGUS, which led him to explore alternative
approaches. In the veterinary community as well as in human medicine, there is increasing interest in herbs and other plants
that have therapeutic applications, and one such plant that shows promise for treating EGUS is the seabuckthorn, Hippophae
rhamnoides.
The seabuckthorn is a deciduous berry-producing shrub that grows in high elevations across Eurasia from the Atlantic coast of
Europe to northwestern China. Since antiquity the plant has been associated with improving the health of horses; in fact, the genus
name Hippophae is Latin for “shining horse,” alluding to the beautiful coats of horses that feed on it.
A liquid equine feed additive derived from seabuckthorn berries, SeaBuck Complete™, is now available, and Dr. Andrews has
been investigating its efficacy in treating and preventing gastric ulcers in Thoroughbreds. “Seabuckthorn berries are highly
nutritious,” said Dr. Andrews, “and are a very good source of vitamins, trace minerals, amino acids, and antioxidants. Extracts
from these berries have been used to successfully treat mucosal injury in humans and rats, so we are looking at this product
as an alternative to omeprazole in treating EGUS.” In a small study involving eight adult female horses, he found that SeaBuck
Complete™ seemed to prevent existing ulcers from worsening under stress, which commonly happens among performance
horses.
Now he wants to continue investigating the use of seabuckthorn products using SeaBuck™ Gastro Plus, a new product from the
manufacturers of SeaBuck Complete™, to see if his initial findings will be confirmed. He has submitted a pre-proposal for a Morris
Animal Foundation grant to expand his research and hopes to start a follow-up clinical trial in the near future.
In addition, Dr. Andrews will be mentoring a student in the Merial/NIH Veterinary Scholars Program this summer. Alyssa Auer, who
is finishing her first year at Purdue University School of Veterinary Medicine, will be at LSU SVM for a 12-week research program
beginning in May, 2010, and will be working closely with Dr. Andrews on a project entitled, ““Efficacy of seabuckthorn berry pulp
and oil on treatment and prevention of gastric ulcers in horses.”
If Dr. Andrews’s research confirms the value of seabuckthorn extracts in managing EGUS, it will be a boon for horses and their
owners alike. At recommended doses, seabuckthorn supplements cost much less than the price of a course of omeprazole,
making it more likely that horse owners will be able to continue treatment for as long as necessary. “We expect seabuckthorn-
treated horses will have significantly fewer and less severe gastric ulcers on endoscopic examination than untreated controls,” he
said. “We believe this research is likely to produce an immediate beneficial outcome on the morbidity caused by gastric ulcers in
performance horses.”
GASTROINTESTINAL
60
DISASTER RESPONSE
Experiential learning in disaster response for veterinary students and veterinarians
Authors/Investigators
McConnico RS, Wolfson W, Poirrier RA, Taboada J, Woodward M, Loftin P, Cartmill J, Huff J, Keowen M, Garza F
The Louisiana State University School of Veterinary Medicine has taken advantage of its physical presence amidst a disaster
prone region of the United States (US) to incorporate veterinary students, faculty veterinarians, and staff as first responders as a
means of experiential training for animal disaster response. Through a solid partnership with the Louisiana State Animal Response
Team (LSART) and others including the LSU Fire and Emergency Training Institute (LSU FETI) and humane organizations, this
veterinary school has developed a disaster response program that includes animal emergency response planning, evacuation,
sheltering, emergency triage, and technical rescue expertise. Specific debriefing sessions from each of the disaster response
scenarios have enabled the emergence of a functional all-hazards disaster response plan and specialized responder training.
Over the past decade, this team has worked closely with local and regional resource providers to develop improved response
activities to care for animals and the people who care for them including Tropical Storm Allison (2001), Hurricanes Katrina & Rita
(2005), Hurricanes Rita 7 Ike (2008), North Louisiana Flood (2009), and the Deep Water Horizon Gulf Oil Spill (2010). These
experiential learning opportunities by way of organized community engagement have provided rigorous training opportunities for
veterinary students and veterinarians at LSU surrounding area. These recent response activities where veterinary students and
veterinarians were able to provide the work force and work first hand with certified emergency responders to provide and training
activities demonstrate a successful model for veterinary training providing robust training experiences and subsequent course
development to address identified gaps in veterinary disaster response training.
61
SURGERY
LARMoF’s Procedure for Treatment of Cribbing in Horses: Is it working?
Authors/Investigators
D.J. Burba, DVM, Dipl. ACVS, Anna Guzman
Cribbing, also referred to a crib-biting is considered an acquired stereotypic oral behavior
that affects 4 to 5 % of the horse population. The exact cause of cribbing is not known.
However, multiple factors may contribute to the reason a horse wants to crib. These
include boredom, frustration, stress, diet, illness, neurological, and possibly hereditary.
Cribbing is considered for the most part a nuisance; however there are potential sequelae
that may develop as a result of cribbing. A cribbing horse may lose weight, perform poorly,
have frequent bouts of colic, and develop excessive wear of the upper incisors. These
sequelae develop as a result of the frequency of the cribbing behavior.
There have been various non-surgical as well as surgical procedures have been described with various success rates. Dr. Forssell
described the first surgical procedure to alleviate cribbing. This entailed resection of portion of the paired sternomandibularis,
omohyoideus, and sternothyrohyoideus muscles. Unfortunately this resulted in a poor cosmetic outcome which was unappealing
to owners. A modification to this technique was published in which resection of the ventral branch of the spinal accessory nerves
were performed in lieu of the sternomandibularis muscles.
Articles were published reporting the success of surgical procedures by numerous surgeons. The success rates were not
impressive in most of the reports. This may be due to a tendon-like fibrous union developing between the cut ends of the muscles.
It is hypothesized that this fibrous union can be reduced by a more rostral transection of the insertion of the sternohyoideous
and omohyoideus. A more rostral transection of the insertion of these muscles was reported in 2002 from LSU EHSP in which
a revision of the modified Forssell’s techniques was performed in 10 horses with 100% success. Since then, the procedure has
been performed on a total of 100 horses. The purpose of this study is to look at the success of the laser assisted revised modified
Forssell’s (LARMoF) surgical procedure to alleviate cribbing in horses.
Material and Methods
100 cases were reviewed from November 1994 to July 2010 and included in the study. Case records were reviewed and
information retrieved included duration of cribbing prior to surgery, gender, age, breed, use, and complications post-surgery if
any. A telephone questionnaire was performed as part of the study to gather information from the owner including the long-term
outcome of the surgery. The same surgical procedure previously described was used.
Results
Information from owners was obtained for 82 cases (Table 1). The number of horses that ceased cribbing was 68/82 (83%). Most
of the owners were pleased with the cosmetic outcome of the procedure (81/82, 99%). Of the horses (14/82) that surgery failed
to alleviate cribbing, the time post surgery cribbing behavior resumed ranged from 5 months to 3 years (Table 2). However all
reported less frequent episodes of the behavior. A few of the owners (5/85, 6%) felt that the horse’s performance was altered
negatively post-surgery. This included 2 racing Thoroughbreds, 2 cutting horses, and 1 Warmblood used for dressage.
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Table 1. Information obtained from owners and records of cases in which LARMoF surgery was performed to alleviate
cribbing.
Table 2. Cases in which LARMoF surgery failed to alleviate cribbing.
SURGERY
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SURGERY
Discussion
Results revealed that the LARMoF
technique is more successful in
alleviating cribbing then techniques
previously reported except one (table
3). Hakansson et al, reported an 89%
(92/103 horses) success; however
the Forssell’s technique was used
which caused a cosmetic defect in
neck which is not appealing to owner.
Of the surgeries that failed in our
study, nearly twice as many horses
had been cribbing more than 2 years
compared to cases that had cribbed
less than two years prior to surgery
( 9 vs 5). Complications did occur
in 17/82 (21%). This included post-
op hematoma/seroma formation,
prolonged drainage as defined as
more than 2 weeks post surgery,
post-operative infection, and partial
dehiscence. Of these, surgery failed
to alleviate cribbing in 10 horses. So it
appears complications also increase
the incidence that surgery will not alleviate cribbing. This may be due fibrous tissue formation contributing to the horse’s ability to
continue cribbing. It was interesting to note that box stall confinement was part of the daily life of all cases reported.
Conclusion
LARMoF is an effective technique for alleviating
cribbing in horses. Further work is needed to reduce the
complications associated with the surgery.
Table 3. Surgical success rate of previous reports of
alleviating cribbing in horses.
F=Forssell’s technique
MF=Modified Forssell’s technique
N=Neurectomy alone technique