ENZYMES

ENZYMES

Safety in foodstuff represents today a priority and winemakers should be able to ensure it.EVER has always been attentive to precision and safety of its solutions, so to guarantee the success to the achievement of its own targets. By following this philosophy, EVER srl has obtained the ISO 9001/2000 (N. IT06/0451) certification and proposes now EVERZYM, a complete range of winemaking enzymes, reliable and with the aim of offering to the winemaker all the possibilities to reach its targets in quality vinification.

This is why EVERZYM distinguishes itself for:

Being in compliance with CERegulation 1332/2008;

not deriving from Genetically Modified Organisms;

being immune from antibiotic activity,ochratoxin A and aflatoxin;

purity and specificity of its activity,a sit is produced with a naturally lowlevel of cinnamyl-esteraseand anthocyanase activity;

high concentration, which allows for theuse in micro dosage, with optimal yields.

The mashing, the maceration and refining do represent

crucial moments in which the enological aim is defined.

The modern tendency towards the “conservative

techniques”, as opposed to the previous “subtractive

techniques”, has directed us to study enzymes with

specific primary and secondary activities, with the aim of

taking advantage of the grape’s own characteristics and to

improve the stability and the filterability of wines.

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160’ FROM ENZYME ADDITION (2 g/hl)

Turbidity values (NTU) on Trebbiano must

NTU

Everzym LiquidEnzyme 1Enzyme 2

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AFTER CENTRIFUGE AND 60’FROM ENZYME ADDITION (2 G/HL)

FROM THE BOTTLE TOP, AFTER 160’FROM ENZYME ADDITION (2 G/HL)

Everzym VRTEnzyme 1Enzyme 2

NTU

Turbidity values (NTU)on Muscat must

EVERZYM LIQUIDPectolytic, liquid and concentrated enzyme with secondary activities; it is ideal in treatment of musts before flotation/static clarification with the following advantages:

Ready for use

Low dosage

Cost effective

Ideal for flotation

EVERZYM LIQUID, in conclusion, is active on all soluble

and non soluble pectins, accelerating the clarification of

most of the juices with linear pectins (e.g. Trebbiano).

EVERZYM GPC

EVERZYM VRTConcentrated, liquid enzyme preparation specific for must proceeding from aromatic cultivars where it accelerates the clarification even in critical conditions of pH and temperature. It allows obtaining more limpid musts and more compact lees. It is active both on soluble and insoluble pectins and it is recommended: On free-run juice from white and aromatic

grapes, immediately after the soft pressing,

to accelerate its clarification, the following

flotation, centrifugation, filtration, etc

On musts of partially dried or macerated

grapes for a faster hydrolysis of pectins

On musts low in pH and low temperature

(early harvest)

Micro granulated, concentrated enzyme preparation, indicated for a quick reduction of viscosity in musts proceeding from white grapes.Active both on soluble and insoluble pectins and it’s used:

On free-run juice of white grapes,

after drip off-pressing or soft press, for a faster

clarification, flotation, centrifugation etc.

It acts at low dosage tank to its concentration

in pectinase and pectin-lyase activities.

EVERZYM XPLNO ENZYME

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Free-run juice1ª pressing2ª pressing

EFFICACY OF THE TREATMENT WITH EVERZYM XPL

TOTAL ANTHOCYANS FREE ANTHOCYANS

ANTHOCYANS BOUND TO TANNINS

Ef�cacy of EVERZYM THERMO IN FDP

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FDPFDP + Everzym Thermo

EVERZYM XPLLiquid enzyme with high pectinase activity, recommended on grape and mash because of its specific activity on the pectins present in the pulp (fast and complete hydrolysis).

It accelerates the extraction of free-run juice, increasing its yield and limiting the production of lees and turbidity of the must

It maintains the skin integrity but, in themeantime, it favors the extraction of aromatic precursors thanks to a better and more delicate washing on skins

Suggested for grapes in which we want to limitthe color extraction (e.g. Pinot Grigio)

Enhances the musts filterability

Efficient even in critical conditions, such as: pH ≤ 3 Temperature ≥ 7°C. Sulfited grapes, mash and grapes.

Efficient in early harvests and on poorly ripe grapes mature

EVERZYM THERMOLiquid enzyme with high pectinase concentration, ideal for the application in THERMO VINIFICATION and in FLASH DÉTENTE, techniques that, besides positively impacting the liberation of aromatic compounds, Polyphenols and polysaccharides, also strongly increase the pectins extraction and consequently the must’s viscosity and turbidity. Inactivation of natural enzymes caused by the heat and the relevant quantity of extracted pectins cause a serious difficulty in clarification as well as in filtration.EverzymThermo works in synergy with heat-extraction techniques:

It optimizes the effect of highest expression of

the aromatic potential

It positively affects the color extraction

It expedites pectins hydrolysis

It favors the clarification, centrifugation and

filtration

hL of merlot �ltered with IMMER M7

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EverzymCOLOR

EVERZYM LCLREFERENCE

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COLORINTENSITY

MACERATION OF MERLOT GRAPES

EVERZYM COLORMicro granulated enzyme preparation, rich in secondary activities, designed for the red vinification with medium long macerations, where it will: Accelerate the extraction of stable color

Solve problems connected to foam formation

in some cultivar

Facilitate the clarification

Enhances the filtration yields

Positively impacts in “volume”, softness,

chemical-physical and sensory stability, thanks

to its secondary ß-glucanase activity

and fairly reduces the use of bentonite to all

advantage of aromas.

EVERZYM LCLLiquid and concentrated pectinase preparation designed for red vinification with brief maceration, where:

It will extract color from skins in a faster way

It accelerates clarification

It improves filtration yields

It positively participates to the clarification of must,

mash and in wine obtained through vigorous pressing.

5 g/hL Everzym AROM on TRAMINER

Geraniol

Citronellol

7 day14 dayControl

Linalool

NerolTerpineol

Liquid pectolytic enzyme with strong ß-glucanase activity and moderate protease and ß-glycosidase secondary activities.Applications:

On fine lees it does accelerate the lysis of

yeast and bacteria, reducing the refining time;

It increases the complexity and the aromatic

impact;

It improves filterability of musts and wines,

both

in case of grapes affected by Botrytis and in

presence of complex pectin structures.

It results interesting on:

fermentation of difficult musts (expedites the

clarification)

second fermentations (perlage, complexity)

regeneration of filters and cartridges

LIQUID FORM

LISOZIMA

EVERZYM AROM

EVERZYM SURLIES

Granular pectolytic enzyme, with secondary ß-glycosidase activity, helpful for developing the entire aromatic potential asset that from grape has passed into the wine.

This treatment is suggested at end of fermentation, when

the sugar content is <5g/L, given its inhibitive capability

towards this enzyme. The granular form makes it more

stable over the time.

Pure enzyme preparation in granular form, obtained from egg albumen. Lysozyme acts by breaking the cell membrane of the Gram+ bacteria (lactic bacteria) thus reducing the microbial count. This product is used on must or wine with the aim of:

Prevent the acidic hints and the spontaneous

“Malo-Lactic Fermentation” done by indigenous

bacteria

Facilitate alcoholic fermentation by reducing

the opposing effect of lactic bacteria on yeast

(on which it express no inhibition whatsoever)

Reduces the dosage of SO2

Grape/mash White

Rosé

Red

Wine

Must

EVERZYMGPC

EVERZYMXPL

EVERZYMVRT

EVERZYM LIQUID

EVERZYM COLOR

EVERZYMLCL

EVERZYM THERMO

EVERZYMSUR LIES

EVERZYM AROM

LISOZIMA

Technological advantage/ point of strength

granulated enzyme at quick dissolu-tion; max FAN, efficient in clarification

maximum yield in free-run juice; aromas extraction

fast and effi-cient clarifica-tion of difficult musts, rich in branching pectins

efficient in mustclarification

riduces foam formation of must and wine, helps in sta-bilizing color and enhances filterability

accelerates color extrac-tion in brief macerations

optimizes the yield of the heat treatment; accelerates clarification of must after thermo-vinifi-cation

enhances the gustative and aromatic complexity; improves filterability and increases pro-tein stability

aromas libe-ration

prevention and control of MLF

Activity pectinase rich in pectin lyase, arabanase

pectinase rich in poly-galac-turonase

pectinase, arabanase, hemicellulase

pectinase, arabanase

pectinase, hemicellulase, protease,ß-glucanase

pectinase, hemicellulase, cellulase

pectinase rich in pectin lyase

ß-glucanase, ß-glycosidase, protease

ß-glycosidase Lysozyme

Color /moment of use

Pre-fermenta-tive Maceration • •

Maceration • •Pressing • • • •Chiarifica statica • • • •Static sedi-mentation • • •Thermo-vini-fication /Flash Détente

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Filtration • •Aroma Extrac-tion / liberation of aromatic precursors

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Stabilization • •Prevention MLF •Dosage g/hL

0,5-2 1-2 1-3 1-3 1-5 1-3 1-3 3-10 1-5 20-50*

Formulation GRANULATED LIQUID LIQUID LIQUID GRANULATED LIQUID LIQUID LIQUID GRANULATED GRANULATED

Key:*With max dosage allowed: 50 g/hL

www.ever.it

Azienda con Sistema di Gestione della Qualitàcertificato ISO 9001:2008 - certificato N. IT06/0451

ALCOHOL TESTPectolytic enzymes hydrolyze the pectins. Their activity is measurable with a simple test known as “Alcohol Test”; besides making evident the effi-ciency of the treatment, it also can be used to assess the correct dosage of enzyme for a safe result, without any waste of product.

Test tubes of 20 ml

Ethanol acidified with 1% of HCl

Must/wine to be tested

- put 5 ml of acidified ethanol in the test tube

- add 5 ml of juice or wine

- gently turn the tube upside down for 3 times

- wait for 5/10 minutes for the evaluation

formation of gel on the top of he test tube: PECTINS

floccules in suspension: PARTIALLY HYDROLYZED PECTINS

no flocculation or precipitation: HYDROLYZED PECTINS

Materials: Method: Results and interpretation:

A goal can be considered as achieved only when we possess adequate means of control.EVER, in a context of comparison and measurement of results, reminds that it is possible to single out a correct dosage and to assess the efficiency of the enzyme treatments through simple tests that can be performer in the winery lab.

PRECISION AND SAFETY......CONTROLLABLE!

EVER s.r.l. Via Pacinotti, 37 30020 PRAMAGGIORE (VE) ITALY tel. +39 0421 200 455 r.a. fax +39 0421 200 460 e-mail: info@ever.it