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Environmental Express WebinarEnvironmental Express WebinarMethod 1664 –
Oil and Grease LightningOil and Grease Lightning
02/02/2016
What is Oil & Grease?What is Oil & Grease?
Method-Defined AnalyteMethod Defined Analyte
HEM vs. Oil & Grease Method modifications are extremely limited Method modifications are extremely limited Modifications cannot alter the chemistry of the
method Modifications cannot adversely affect HEM
recoveries
From EPA 413.1 to EPA 1664
Replaced Freon with n-hexane 1664 d t d i 1999 d t
From EPA 413.1 to EPA 1664
1664 adopted in 1999 and we are now up to Revision B
EPA withdrew all Freon methods from 40 CFR EPA withdrew all Freon methods from 40 CFR part 136 in 2007
1664 is now the only method approved for y pptesting wastewater for oil & grease
What did Method Change Mean?What did Method Change Mean?
Oil & Grease still a “method-defined analyte” though is id d H E t t bl M t i l (HEM)now considered Hexane Extractable Material (HEM)
The term "total petroleum hydrocarbons" (TPH) has been replaced by "non-polar material" (NPM) to avoid replaced by non polar material (NPM) to avoid confusion with TPH measurements by other methods
The requirement for a matrix spike duplicate (MSD) has b h d ibeen changed to a suggestion
The size of an analytical batch has been increased to a maximum of 20 samples per discharge or waste streammaximum of 20 samples per discharge or waste stream
Use of solid-phase extraction (SPE) has been allowed without a demonstration of equivalency
LLE vs. SPELLE vs. SPE
Liquid-Liquid Extraction Solid-Phase Extractionq q
3 shakes of 30 mL of solvent
Little solvent usage (as little as 40 – 50 mL)solve t
Higher analyst exposure to n-hexane
l ttle as 0 50 ) Less solvent = less
exposure Prone to emulsions Relies on analyst
Less labor intensive Frees up analysts to do
precision and accuracy Hexane floats!
other things in the lab More consistent results
Solid Phase Extraction (SPE)Solid Phase Extraction (SPE)
SPE allowed in Revision A of Method 1664 without a demonstration of equivalency
Utilizes an extraction disk impregnated with C-18 as the absorbent resinabsorbent resin
The C-18 in the disk is “activated” with a polar solvent (methanol) prior to sample filtrationS l i ll d th h th di k hi h t i Sample is vacuum pulled through the disk which retains the HEM
HEM is then eluted off of the disk with n-hexane Hexane + HEM collected in a pre-weighed flask or dish
and the n-hexane driven off by heat to leave the HEM behindbehind
Important things about SPEImportant things about SPE
Extraction disc is not analyte yspecific
C-18 used to make discs will adsorb antibiotics, pesticides, herbicides, surfactants, and vitamins
Solvent used for elution is the determining factor
Capacity of the disc –‘parking lot’ analogy
Manual SPEManual SPE
Assembling the StepSaverAssembling the StepSaver
Assembling the StepSaverAssembling the StepSaver
Assembling the StepSaverAssembling the StepSaver
Assembling the StepSaverAssembling the StepSaver
Assembling the StepSaverAssembling the StepSaver
Assembling the StepSaverAssembling the StepSaver
Assembling the StepSaverAssembling the StepSaver
Assembling the StepSaverAssembling the StepSaver
Using the StepSaverUsing the StepSaver
Disc Activation – Alternate option, UltraPrepoption, UltraPrep
Remove methanol, replacewith DI waterwith DI water
Vacuum ‘filter’ sample
Dry disc
El t ith h Elute with hexane
Evaporate the hexane
Potential Problems with SPE
Particulate laden samples
Potential Problems with SPE
Extraction disc activation
Residual analyte in the sample bottle
Automated SPEAutomated SPE
Environmental Express SKUs -- Equipment StepSaver Systems StepSaver Systems
Part # DescriptionG1047 1 Station 47mm w/ Glass Funnel
Part # DescriptionG1121 1 Station PVC Disposable FunnelG1047 1 Station. 47mm w/ Glass Funnel
G1090 1 Station. 90mm w/ Glass Funnel
G5747 1 Station. 47mm no funnel
G1101 1 Station. PVC. Glass Funnel
G1103 3 Station. PVC. Glass Funnel
G1121 1 Station. PVC. Disposable Funnel
G1123 3 Station. PVC. Disposable Funnel
G1127 7 Station. PVC. Disposable Funnel
G2121 1 Station. Stainless Steel. Disposable Funnel
G2123 3 Station. Stainless Steel. Disposable Funnel
G1107 7 Station. PVC. Glass Funnel
G2101 1 Station. Stainless Steel. Glass Funnel
G2103 3 Station. Stainless Steel. Glass Funnel
G2106 6 Station. Stainless Steel. Glass Funnel
GUP1101 1 Station PVC Glass Funnel With UltraPrep® funnels
G2126 6 Station. Stainless Steel. Disposable Funnel
GUP1121 1 Station. PVC. Disposable Funnel With UltraPrep® Funnels
GUP1123 3 Station. PVC. Disposable Funnel With UltraPrep® Funnels
GUP1127 7 Station. PVC. Disposable Funnel With UltraPrep® Funnels
GUP2121 1 St ti St i l St l Di bl F l With Ult P F lGUP1101 1 Station. PVC. Glass Funnel. With UltraPrep® funnels
GUP1103 3 Station. PVC. Glass Funnel. With UltraPrep® funnels
GUP1107 7 Station. PVC. Glass Funnel. With UltraPrep® funnels
GUP2101 1 Station. Stainless Steel. Glass Funnel. With UltraPrep® funnels
GUP2103 3 Station. Stainless Steel. Glass Funnel. With UltraPrep® funnels
GUP2121 1 Station. Stainless Steel. Disposable Funnel With UltraPrep® Funnels
GUP2123 3 Station. Stainless Steel. Disposable Funnel With UltraPrep® Funnels
GUP2126 6 Station. Stainless Steel. Disposable Funnel With UltraPrep® Funnels
GUP2106 6 Station. Stainless Steel. Glass Funnel. With UltraPrep® funnels
Environmental Express SKUs -- DiscsEnvironmental Express SKUs Discs
Part # DescriptionG5300MM UltraLow™ – 47mm C18 discs without prefilters. 20pkp p
G5347MM UltraLow™ – 90mm C18 discs without prefilters. 15pk
G5390MM UltraLow™ – 100mm C18 discs without prefilters. 15pk
G5047MM UltraFlow™ – 47mm C18 discs with prefilters. 20pk
G5090MM UltraFlow™ – 90mm C18 discs with prefilters. 15pk
G5100MM UltraFlow™ – 100mm C18 discs with prefilters. 15pk
G5127 StepSaver disposable funnels with 47mm UltraFlow™ discs. 20pk
G5260 Filter assembly with UltraFlow™ 47mm disc. No Funnel. 100pk
GUP047MM UltraPrep® – 47mm Preactivated discs with prefilters. 20pk
GUP090MM UltraPrep® – 90mm Preactivated discs with prefilters. 15pk
GUP100MM UltraPrep® – 100mm Preactivated discs with prefilters. 15pk
GUPF47EE StepSaver disposable funnels with 47mm UltraPrep® discs. 20pk
FG650100MM 100mm Thick glass fiber prefilters. 50pk
FG65041MM 41mm Thick glass fiber prefilters 50pkFG65041MM 41mm Thick glass fiber prefilters. 50pk
FG65047MM 47mm Thick glass fiber prefilters. 50pk
FG65090MM 90mm Thick glass fiber prefilters. 50pk
Environmental Express SKUs -- AccessoriesEnvironmental Express SKUs Accessories
Part # DescriptionG2018 Standard 5mL tubes 40mg HEM 20pk
Part # DescriptionLCCC08002 Reagent n-Hexane 2 5LG2018 Standard. 5mL tubes. 40mg HEM. 20pk
G3004 Standard. 10mL tubes. 4mg HEM. 20pk
G3020 Standard. 30mL bottles. 40mg HEM. 9pk
G3023 MDL Standard. 10mL tubes. 7mg HEM. 20pk
G3024 MDL Standard. 10mL tubes. 5mg HEM. 20pk
LCCC08002 Reagent. n Hexane. 2.5L
LCCC08002C Reagent. n-Hexane. 4x 2.5L
LCCC08003 Reagent. Methanol ACS. 2.5L
LCCC08003C Reagent. Methanol ACS. 4x2.5L
LCCC08004 Deionized Water. 2.5L
G3025 Standard. 10mL tubes. 40mg HEM. 20pk
G3026 MDL Standard. 10mL tubes. 10mg HEM. 20pk
G7000 QuikVap evaporating system. 120 volt
G7000-240 QuikVap evaporating system. 240 volt
LCCC08004C Deionized Water. 4x2.5L
LCCC08005 Reagent. n-Hexane. For smart bottle rack system. 4L
LCCC08005C Reagent. n-Hexane. For smart bottle rack system. 4x4L
LCCC08006 Reagent. Methanol ACS. For smart bottle rack system. 1L
G1200 Single-place stainless steel manifold for Oil & Grease
G1210 Single-place PVC manifold for Oil & Grease
G1250 3-Place stainless steel manifold
G1260 3-Place PVC manifold for Oil & Grease
G1270 7 Pl PVC if ld f Oil & G
LCCC08006C Reagent. Methanol ACS. For smart bottle rack system. 6x1L
LCCC08007 Deionized water for smart bottle rack system. 1L
LCCC08007C Deionized water for smart bottle rack system. 6x1L
G1270 7-Place PVC manifold for Oil & Grease
G1300 6-Place stainless steel manifold for Oil & Grease
G3050 1/8 HP vacuum pump. 25.5” Hg. 115VAC
Thank you for attendingThank you for attendingQuestions?
Email us at [email protected] OR call us 800.343.5319http://www.envexp.com/products/1-Wet_Chemistry/SS-Oil_and_Grease_EPA_1664
02/02/2016
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