ENHANCEMENT OF IMMUNITY AGAINST COCCIDIOSIS BY THE USE OF AN ADJUVANTED SOLVENT (HIPRAMUNE T®)

Dr. Ong Shyong Wey

Regional Poultry Technical and Marketing Specialist (Asia & Oceania)

HIPRA

World Veterinary Poultry Association (India)

The etiological agent of poultry coccidiosis

Eimeria species causing coccidiosis in Gallus gallus (chart P.L. Long & W.M.Reid, 1982):

- Eimeria acervulina

- E. maxima

- E. mitis*

- E. praecox*

- E. tenella

- E. brunetti

- E. necatrix

* Cause subclinical coccidiosis

Both E.mivati and E.hagani are considered nomina dubia (organisms for which the evidence is insufficient to recognise the species).

BREEDERS/LAYERS

BROILERS

COCCIDIOSIS IMMUNITY

Montanide-type adjuvant ↑Th1 response (↑IL-2, ↑IFN-Ƴ). Better vaccine performance, higher

effectiveness under suboptimal conditions

CLT

CD8+

Essential for innate and adaptive immunity against protozoa

IL-2

IFN-Ƴ

MONTANIDE ADJUVANT

activates , enhance the destruction of infected cells

Proliferations of TH, TH1 and TH2 cells

EVALON®

Live attenuated strains:

E.acervulina 003

E.brunetti 034

E.maxima 013

E.necatrix 033

E.tenella 004

HIPRAMUNE T®

Colouring agent

Aroma

Montanide-typeadjuvant

VACCINE + ADJUVANTED SOLVENT

STUDY OBJECTIVES

• Study the mechanisms of an adjuvant modulating

the immune mechanisms that are triggered in the

host after coccidiosis vaccination.

• Study the important markers of Th1 and Th2

responses.

STUDY DESIGN

STUDY DESIGN

MATERIAL&METHODS

5 birds/ subgroups

sample at

Day 7, 23 and 43

post vaccination

MATERIAL&METHODS

5 birds/ subgroups

sample at

Day 7, 23 and 43

post vaccination

MATERIAL&METHODS

Step 1. Collection and

cleaning of sample in

medium + antibiotics

Step 2. sample incubation medium

+ Eimeria whole antigen +

antibiotics

MATERIAL&METHODS

Step 3. Filtration

Step 4. Fixation with acetone

and staining using monoclonal

antibodies marked with

fluorescein

MATERIAL&METHODS

Step 5. Study of

samples using

flow-cytometryC

om

ple

xity

Intensity of Fluorescense

(IL-2, IFN-Ƴ, IL-4 or IL-10)

Step 6. Evaluation of the % of

positive lymphocytes

MUCOSA DAY 7

MUCOSA DAY 23

PEYER’S PATCHES DAY 23

,IMMUNOSTAINING IL-2, DAY 23

,IMMUNOSTAINING IL-2, DAY 23

,IMMUNOSTAINING IFN-Ƴ, DAY 23

,IMMUNOSTAINING IFN-Ƴ, DAY 23

MUCOSA DAY 43

PEYER´S PATCHES DAY 43

,IMMUNOSTAINING IL-2, DAY 43

,IMMUNOSTAINING IFN-Ƴ, DAY 43

,SUMMARY

1. HIPRAMUNE® T switches the vaccine immunity towards a stronger Th1 response(↑IL-2, ↑IFN-Ƴ)

2. Without HIPRAMUNE® T, the vaccine would have a stronger Th2 response (↑IL-4, ↑IL-10)

3. In the tissue, HIPRAMUNE® T induces the formation of lymphoid aggregates of T-cells that express IL-2 and IFN-Ƴ

HIPRAMUNE® T + vaccine is able to polarize the immune response towards a

more intenseTh1 response than the live vaccine without HIPRAMUNE® T

,CONCLUSIONS

1. A stronger Th1 response boosts the performance of the coccidiosis vaccine. Better efficacy in worst conditions

2. Pre-clinical studies and on-field results confirm that Duration of Immunity is extended to 60 weeks

3. A stronger Th1 response, does not compromise the Th2 response as it has been seen in field studies

Ong Shyong WeyRegional Poultry Technical and Marketing Specialist

(Asia & Oceania)Shyongwey.ong@hipra.com

+6012-6894569

WVPA (India) ConferenceAdvances in Poultry Science for One Health

28th February 2020, Bangalore – Indiafor Membership and other info please Log into

www.wvpaindiaconference.com

www.wvpa.inor email to : dr.barman@gmail.com