ENHANCEMENT OF DEGREE OF DEACETYLATION OF CHITIN IN CHITOSAN PRODUCTION

STEPHENIE AK KALUT

A thesis submitted in fulfillment of the requirements for the award of the degree of

Bachelor of Chemical Engineering

Faculty of Chemical Engineering and Natural Resources Universiti Malaysia Pahang

MAY 2008

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I declare that this thesis entitled “Enhancement of Degree of Deacetylation of Chitin in

Chitosan Production” is the result of my own research except as cited in the references.

The thesis has not been accepted for any degree and is not concurrently submitted in

candidature of any other degree.

Signature : …………………….

Name : Stephenie A/K Kalut

Date : May 2008

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To my beloved mother, Silia ak Mandung, and father, Kalut ak Legan and also my siblings.

For Rome was not built in a day, you were there to help me make it through.

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ACKNOWLEDGEMENT

I wish to express my sincere appreciation to my superisor, Mrs Ruwaida binti

Abdul Rasid, for her guidance, encouragement, patience, and understanding throughout

the course of this research. I am also thankful to her for providing me the research topic

and valuable suggestions. I would like to thank Mr Mohd Anuar bin Hj. Ramli for

helping me during my experiment progress and not only for providing me the research

facilities but also for imparting valuable knowledge that I used in this research.

My sincere gratitude and appreciation goes to my dear collegues and friends, Siti

Nurshuhada binti Mat Yusof, Fauziah binti Hairan, and Nurul Yazlin binti Yussuf who

have assisted and supported me throughout the most rigorous phase of my research with

their expertise, wisdom, and friendship. Last but not least, I thank my mama and papa

who are the sunshine of my life for their encouragement and support throughout every

endeavor of mine. It is to them that this work is dedicated. May God bless all of you.

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ABSTRACT

Chitosan is made from crustacean shells by a chemical process involving

demineralization, deproteinization, decolorization, and deacetylation. The process of

deacetylation involves the removal of acetyl groups from the molecular chain of chitin,

leaving behind a complete amino group (-NH2) and chitosan versatility depends mainly

on this high degree chemical reactive amino groups. The purpose of this research is to

observe the parameters that can enhance the degree of deacetylation of chitosan

production to the highest percentage. The observed parameters are the temperature of

heating, concentration of sodium hydroxide, and the time of heating. The obtained chitin

was converted into the more useful soluble chitosan by reaction with sodium hydroxide

(NaOH) solution of various concentrations, then the alkaline chitin was heated in an

autoclave with different time and temperature of heating which dramatically reduced the

time of deacetylation. The method used to determine the degree of deacetylation of

chitosan is the linear potentiometric titration. From the result, the highest degree of

deacetylation can be achieved at the temperature of 134oC, and 70% concentration of

sodium hydroxide with DDA% of 98.38% and 98.79% respectively. It took only 10

minutes to also achieve highest degree of deacetylation, 89.05%. In conclusion, the

increasing of temperature and concentration of sodium hydroxide will increase the

degree of deacetylation of chitin. The increasing of time of heating will decrease the

degree of deacetylation.

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ABSTRAK

Chitosan terhasil daripada kulit udang melalui proses kimia yang melibatkan

proses “demineralization” iaitu penyingkiran bahan mineral, “deproteinization” iaitu

penyingkiran bahan berprotin, “decolorization” iaitu penyingkiran bahan berwarna, dan

“deacetylation” iaitu penyingkiran kumpulan ikatan polimer N-acetyl. Tujuan utama

kajian ini dijalankan adalah untuk mengkaji factor-faktor yang meningkatkan peratusan

proses penyingkiran ikatan polimer N-acetyl iaitu suhu pemanasan, kepekatan alkali

iaitu natrium hidroksida, dan masa pemanasan. Chitin yang diperoleh akan ditukar

kepada chitosan yang mempunyai lebih banyak kegunaan dan boleh melarut dalam air

dengan baik. Ini dilakukan dengan mencampurkan chitin dengan larutan natrium

hidroksida dengan kepekatan yang berbeza dalam masa dan suhu pemanasan yang

berbeza dan membiarkan ia bertindakbalas. Selepas itu, chitin beralkali itu dipanaskan di

dalam alat pemanas “autoclave” di mana proses pemanasan ini akan mempercepatkan

masa untuk proses penyingkiran ikatan polimer N-acetyl. Kaedah yang digunakan untuk

mengira peratusan penyingkiran ikatan polimer N-acetyl itu adalah pentitratan

potensiometrik. Daripada hasil eksperimen yang dijalankan, didapati peratusan yang

paling tinggi diperoleh pada suhu 134oC, dengan peratusan sebanyak 98.38% dan pada

kepekatan natrium hidroksida sebanyak 70% dengan peratusan sebanyak 98.79%. Masa

pemanasan dalam 10 minit pula menghasilkan peratusan penyingkiran yang paling

tinggi iaitu sebanyak 89.05%. Kesimpulannya, semakin tinggi suhu pemanasan dan

kepekatan alkali, semakin meningkat peratusan proses penyingkiran ikatan polimer N-

acetyl di dalam chitosan. Semakin meningkat masa pemanasan pula, semakin rendah

peratusan proses penyingkiran ikatan polimer N-acetyl tersebut.

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TABLE OF CONTENTS

CHAPTER TITLE PAGE

DECLARATION ii

DEDICATION iii

ACKNOWLEDGEMENT iv

ABSTRACT v

ABSTRAK vi

TABLE OF CONTENTS vii

LIST OF SYMBOLS x

LIST OF FIGURES xi

LIST OF TABLES xii

LIST OF APPENDICES xiii

1 INTRODUCTION

1.1 Background Study 1

1.2 Problem Statement 3

1.3 Objectives 3

1.4 Scope of Study 4

2 LITERATURE REVIEW

2.1 Definition and Composition of Chitosan 5

2.2 Characteristics of Chitosan 7

2.2.1 Degree of Deacetylation in Chitosan 8

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2.2.2 Molecular Weight 10

2.2.3 Viscosity 10

2.2.4 Solubility 12

2.2.5 Bulk Density 13

2.2.6 Color 13

2.2.7 Water Binding Capacity and Fat

Binding Capacity 14

2.2.8 Emulsification 15

2.2.9 Antimicrobial Properties 15

2.2.10 Formation of Film 17

2.3 Production of Chitin and Chitosan 18

2.3.1 Isolation of Chitin 19

2.3.2 Deproteinization 22

2.3.3 Demineralization 22

2.3.4 Decolorization 23

2.3.5 Deacetylation 24

2.4 Factors Affecting Production of Chitosan 25

2.4.1 Temperature of Deacetylation 25

2.4.2 Time of Deacetylation and Alkali

Concentration 26

2.4.3 Effect of Treatment Conditions Applied

in Chitin Isolation 26

2.4.4 Atmosphere, Ratio of Chitin to Alkali

Solution, and Particle Size 27

2.5 Determination of Deacetylation Degree 27

2.6 Applications of Chitosan 29

2.6.1 Dietary Suppliments 29

2.6.2 Industrial Wastewater Treatment 30

2.6.3 Medical Applications 31

3 MATERIALS AND METHODS

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3.1 Isolation of Chitin 33

3.2 Purification Process 33

3.2.1 Deproteinization 34

3.2.2 Demineralization 35

3.2.3 Decoloriztion 35

3.2.4 Deacetylation 35

3.2.4.1 Time of Heating 36

3.2.4.2 Temperature of Heating 36

3.2.4.3 Alkaline (NaOH) Concentration 36

3.3 Determination of Deacetylation Degree 37

3.3.1 Linear Potentiometric Titration 37

4 RESULTS AND DISCUSSIONS

4.1 Results 38

4.1.1 Time of Heating in Autoclave 38

4.1.2 Temperature of Heating in Autoclave 40

4.1.3 Concentration of Sodium Hydroxide

(NaOH) 41

4.2 Discussions 42

4.2.1 Time of Heating in Autoclave 42

4.2.2 Temperature of Heating in Autoclave 43

4.2.3 Concentration of Sodium Hydroxide

(NaOH) 43

4.2.4 Limitation of Research 44

5 CONCLUSION

5.1 Conclusion 45

5.2 Recommendation 46

REREFENCES 47

APPENDICES 52

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LIST OF SYMBOLS

> - Bigger than

≈ - Nearly equal to

DDA - Degree of Deacetylation

w/w - weight per weight

w/v - weight per volume

v/v - volume per weight

WBC - Water Binding Capacity

FBC - Fat Binding Capacity

KOH - Potassium hydroxide

HCl - Hydrochloric acid

NaOCl - Sodium hypochloride

NaOH - Sodium hydroxide

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LIST OF FIGURES

FIGURE NO. TITLE PAGE

2.1 The structural formula of chitin and chitosan 6

2.2 The structural formula of chitin and glucose. 6

2.3 Structure of cellulose, chitin, and chitosan 7

2.4 Isolation of chitin 20

2.5 Traditional Crawfish Chitosan Production Flow

Scheme (Modified from No and Meyers, 1995) 21

3.1 Steps of purification process 34

4.1 Effect of time of heating on DDA% 39

4.2 Effect of temperature of heating on DDA% 40

4.3 Effect of NaOH concentration on DDA% 41

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LIST OF TABLES

TABLE NO. TITLE PAGE

2.1 Applications of Chitosan 31

4.1 DDA% of different time of heating 38

4.2 DDA% of different temperature of heating 40

4.3 DDA% of different NaOH concentration 41

xiii

LIST OF APPENDICES

APPENDIX TITLE PAGE

A Calculation of Degree of Deacetylation of Chitosan on various times of autoclave. 52

B Calculation of Degree of Deacetylation of Chitosan on various temperatures of autoclave. 55

C Calculation of Degree of Deacetylation of Chitosan on various concentration of sodium hydroxide in autoclave. 58

D Isolation of chitin processes. 62

E Deacetylation process. 63 F Type of Autoclave 65

CHAPTER 1

INTRODUCTION

1.1 Background of Study

Chitosan is a natural polysaccharide comprising copolymers of glucosamine and

N-acetylglucosamine, and can be obtained by the partial deacetylation of chitin, from

crustacean shells, the second most abundant natural polymer after cellulose. Chitin can

be converted into chitosan by enzymatic means or alkali deacetylation, this being the

most utilized method. During the course of deacetylation, part of polymer N-acetyl links

are broken with the formation of D-glucosamine units, which contain a free amine

group, increasing the polymer’s solubility in aqueous means (Chen & Tsaih, 1998).

Chitosan has been widely used in vastly diverse fields, ranging from waste

management to food processing, medicine and biotechnology. It becomes an interesting

material in pharmaceutical applications due to its biodegradability and biocompatibility,

and low toxicity. Chitosan has found wide applicability in conventional pharmaceutical

devices as a potential formulation excipient. The use of chitosan in novel drug delivery

as mucoadhesive, peptide and gene delivery, as well as oral enhancer have been reported

2

in the literature. Chitosan exhibits myriad biological actions such as

hypocholesterolemic, antimicrobial, and wound healing properties. Since chitosan is a

new substance, it is important to carry out precise standardization for its pharmaceutical

and biomedical applications like other auxiliary substances.

Chitosan can be characterized in terms of its quality, intrinsic properties (purity,

molecular weight, viscosity, and degree of deacetylation) and physical forms.

Furthermore, the quality and properties of chitosan product may vary widely because

many factors in the manufacturing process can influence the characteristics of the final

product. Chitosan is commercially available from a number of suppliers in various

grades of purity, molecular weight, and degree of deacetylation. The variations in

preparation methods of chitosan result in differences in its deacetylation degree, the

distribution of acetyl groups, the viscosity and its molecular weight (Berger et al., 2005).

These variations influence the solubility, antimicrobial activity among other properties,

being that commercial chitosan usually has a deacetylation degree varying from 70% to

95%, and a molecular weight ranging from 50 to 2000 kDa (Rege et al., 2003).

The deacetylation degree is the proportion of glucosamine monomer residues in

chitin. It has a striking effect on the solubility and solution properties of chitin. By

convention, chitin and chitosan are distinguished by their solubility in dilute aqueous

acids such as acetic acid (Muzzarelli, 1977). Chitin does not dissolve in dilute acetic

acid. When chitin is deacetylated to a certain degree (~ 60% deacetylation) where it

becomes soluble in the acid, it is referred to as chitosan. A typical deacetylation process

of chitin involves the reaction of chitin powder or flake in an aqueous 40-50% sodium

hydroxide solution at 100-120°C for several hours to hydrolyze N-acetyl linkages

(Roberts, 1992). Repetition of the process can give deacetylation values up to 98% but

the complete deacetylation can never be achieved by this heterogeneous deacetylation

process without modification. Fully deacetylated (nearly 100%) chitosan can be

3

prepared by the alkaline treatment of a gel form instead of the powder form of chitosan

(Mima et al., 1983).

1.2 Problem statement

The degree of deacetylation could influence the performance of chitosan in many

of its applications. It determines the content of free amino groups in the polysaccharides

and can be employed to differentiate between chitin and chitosan. The process of

deacetylation involves the removal of acetyl groups from the molecular chain of chitin,

leaving behind a complete amino group (-NH2) and chitosan versatility depends mainly

on this high degree chemical reactive amino groups. There are methods available to

increase or decrease the degree of deacetylation. For example, increase either in

temperature or strength of sodium hydroxide solution could enhance the removal of

acetyl groups from chitin, resulting in a range of chitosan molecules with different

properties and hence its applications. Preliminary experiments were carried out by

refluxing chitin in strong NaOH solution at normal atmosphere. The experiments took

more than 20 hours producing low deacetylation content and the reaction was

accompanied by drastic degradation of the final chitosan.

1.3 Objective

The objective of this research is to enhance the degree of deacetylation of chitin in

chitosan production.

4

1.4 Scope of study

The scope of this study covers the effect of temperature, concentration of NaOH

solution, and time of heating in autoclave on the degree of deacetylation.

CHAPTER 2

LITERATURE REVIEW

2.1 Definition and Composition of Chitosan

Chitosan is a fiber-like substance derived from chitin. Chitin is the fiber in

shellfish shell such as crab, lobster and shrimp. It is also found in common foods we eat

such as grain, yeast, bananas, and mushrooms. Chitin, a naturally abundant polymer

consists of 2-acetamido 2-deoxy-β-D-glucose through a β(1 → 4) linkage. In spite of the

presence of nitrogen, it may be regarded as cellulose with hydroxyl at position C-2

replaced by an acetamido group. Like cellulose, it functions as structural

polysaccharides. Its natural production is inexhaustible; arthropods, by themselves,

count more than 106 species from the 1.2 X 106 of total species compiled for animal

kingdom, constitute permanent and large biomass source. The chitin is deproteinized,

demineralized and de-acetylated. It is a dietary fiber, meaning that it cannot be digested

by the digestive enzymes of a person (Razdan A., and Petterson D., 1994). Chitin is a

white, hard, inelastic, nitrogenous polysaccharide and the major source of surface

pollution in coastal areas.

6

Chitin is made up of a linear chain of acetylglucosamine groups while chitosan is

obtained by removing enough acetyl groups (CH3-CO) for the molecule to be soluble in

most diluted acids. This process is called deacetylation. The actual difference between

chitin and chitosan is the acetyl content of the polymer. Chitosan having a free amino

group is the most useful derivative of chitin (No and Meyers, 1992).

Chitin Chitosan

Figure 2.1 The structural formula of chitin and chitosan

Figure 2.2 The structural formula of chitin and glucose.

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2.2 Characteristics of Chitosan

Chitosan is a non toxic, biodegradable polymer of high molecular weight, and is

very much similar to cellulose, a plant fiber.

(a) (b)

(c)

Figure 2.3 Structure of a) chitin, b) chitosan, and c) cellulose.

As seen in Figure 2.3, the only difference between chitosan and cellulose is the

amine (-NH2) group in the position C-2 of chitosan instead of the hydroxyl (-OH) group

found in cellulose. However, unlike plant fiber, chitosan possesses positive ionic

charges, which give it the ability to chemically bind with negatively charged fats, lipids,

cholesterol, metal ions, proteins, and macromolecules (Li et al., 1992). In this respect,

chitin and chitosan have attained increasing commercial interest as suitable resource

8

materials due to their excellent properties including biocompatibility, biodegradability,

adsorption, and ability to form films, and to chelate metal ions (Rout, 2001).

2.2.1 Degree of Deacetylation in Chitosan

The process of deacetylation involves the removal of acetyl groups from the

molecular chain of chitin, leaving behind a compound (chitosan) with a high degree

chemical reactive amino group (-NH2). This makes the degree of deacetylation an

important property in chitosan production as it affects the physicochemical properties,

hence determines its appropriate applications (Rout, 2001). Deacetylation also affects

the biodegradability and immunological activity (Tolaimate et al., 2000).

A sharp nomenclature border has not been defined between chitin and chitosan

based on the degree of N-deacetylation (Rout, 2001). In an earlier study by Rudall

(1963), he reviewed evidences suggesting that approximately one in every six to seven

residues in the chain has a proportion of free amino groups that manifests some

histochemical properties. In any case, the degree of deacetylation can be employed to

differentiate between chitin and chitosan because it determines the content of free amino

groups in the polysaccharides. There are two advantages of chitosan over chitin. The

first one is, in order to dissolve chitin, highly toxic solvents such as lithium chloride and

dimethylacetamide are used whereas chitosan is readily dissolved in diluted acetic acid.

The second advantage is that chitosan possesses free amine groups which are an active

site in many chemical reactions (Knaul et al., 1999).

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The degree of deacetylation of chitosan ranges from 56% to 99% with an average

of 80%, depending on the crustacean species and the preparation methods (No and

Meyers, 1995). Chitin with a degree of deacetylation of 75% or above is known as

chitosan (Knaul et al., 1999). Various methods have been reported for the determination

of the degree of deacetylation of chitosan. These included ninhydrin test, linear

potentiometric titration, near-infrared spectroscopy, nuclear magnetic resonance

spectroscopy, hydrogen bromide titrimetry, infrared spectroscopy, and first derivative

UV-spectrophotometry (Khan et al., 2002).

The infrared spectroscopy method, which was first proposed by Moore and

Roberts (1980), is commonly used for the estimation of chitosan degree of deacetylation

values. This method has a number of advantages and disadvantages. First, it is relatively

fast and unlike other spectroscopic methods, does not require purity of the sample to be

tested nor require dissolution of the chitosan sample in an aqueous solvent (Baxter et al.,

1992). However, the infrared method utilizing baseline for degree of deacetylation

calculation, and as such there may be possible argument for employment of different

baseline which would inevitably contribute to variation in the degree of deacetylation

values. Secondly, sample preparation, type of instrument used and conditions may

influence the sample analysis. Since chitosan is hygroscopic in nature and samples with

lower degree of deacetylation may absorb more moisture than those with higher degree

of deacetylation, it is essential that the samples under analysis be completely dry (Khan

et al., 2001; Blair et al., 1987).

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2.2.2 Molecular Weight

Chitosan is a biopolymer of high molecular weight. Like its composition, the

molecular weight of chitosan varies with the raw material sources and the method of

preparation. Molecular weight of native chitin is usually larger than one million Daltons

while commercial chitosan products have the molecular weight range of 100,000 –

1,200,000 Daltons, depending on the process and grades of the product (Li et al., 1992).

In general, high temperature, dissolved oxygen, and shear stress can cause degradation

of chitosan. For instance at a temperature over 280˚C, thermal degradation of chitosan

occurs and polymer chains rapidly break down, thereby lowering molecular weight

(Rout, 2001). Also, maximal depolymerization caused by utilization of high temperature

or concentrated acids, such as hydrochloric acid followed by acetic acid and sulfurous

acid, results in molecular weight changes with minimal degradation with the use of

EDTA (Rout, 2001). The molecular weight of chitosan can be determined by methods

such as chromatography (Bough et al., 1978), light scattering (Muzzarelli, 1977), and

viscometry (Maghami and Roberts, 1988)

2.2.3 Viscosity

Just as with other food matrices, viscosity is an important factor in the

conventional determination of molecular weight of chitosan and in determining its

commercial applications in complex biological environments such as in the food system.

Higher molecular weight chitosans often render highly viscous solutions, which may not

be desirable for industrial handling. But, a lower viscosity chitosan obtained from

crawfish waste as shown in this thesis research may facilitate easy handling.

11

Some factors during processing such as the degree of deacetylation, molecular

weight, concentration of solution, ionic strength, pH, and temperature affect the

production of chitosan and its properties. For instance, chitosan viscosity decreases with

an increased time of demineralization (Moorjani et al., 1975). Viscosity of chitosan in

acetic acid tends to increase with decreasing pH but decrease with decreasing pH in HCl,

giving rise to the definition of ‘Intrinsic Viscosity’ of chitosan which is a function of the

degree of ionization as well as ion strength. Bough et al. (1978) found that

deproteinization with 3% NaOH and elimination of the demineralization step in the

chitin preparation decrease the viscosity of the final chitosan products. Moorjani et al.

(1975) also stated that it is not desirable to bleach the material (i.e., bleaching with

acetone or sodium hypochlorite) at any stage since bleaching considerably reduces the

viscosity of the final chitosan product.

Similarly, No et al. (1999) demonstrated that chitosan viscosity is considerably

affected by physical (grinding, heating, autoclaving, ultrasonication) and chemical

(ozone) treatments, except for freezing, and decreases with an increase in treatment time

and temperature. Chitosan solution stored at 4˚C is found to be relatively stable from a

viscosity point of view (No et al., 1999). The effect of particle size on the quality of

chitosan products was investigated by Bough et al. (1978), who reported that smaller

particle size (1mm) results in chitosan products of both higher viscosity and molecular

weight than those of either 2 or 6.4 mm particle size. They further enumerated that a

larger particle size requires longer swelling time, resulting in a slower deacetylation rate.

But, in contrast, Lusena and Rose (1953) reported that the size of chitin particle within

the 20-80 mesh (0.841-0.177 mm) range had no effect on the viscosity of the chitosan

solutions.

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2.2.4 Solubility

While chitin is insoluble in most organic solvents, chitosan is readily soluble in

dilute acidic solutions below pH 6.0. Organic acids such as acetic, formic, and lactic

acids are used for dissolving chitosan. The most commonly used is 1% acetic acid

solution at about pH 4.0 as a reference. Chitosan is also soluble in 1% hydrochloric acid

but insoluble in sulfuric and phosphoric acids. Solubility of chitosan in inorganic acids is

quite limited. Concentrated acetic acid solutions at high temperature can cause

depolymerization of chitosan (Roberts and Domszy, 1982). Above pH 7.0 chitosan

solubility’s stability is poor. At higher pH, precipitation or gelation tends to occur and

the chitosan solution forms poly-ion complex with anionic hydrocolloid resulting in the

gel formation (Kurita, 1998).

The concentration ratio between chitosan and acid is of great importance to

impart desired functionality (Mima, 1983). At concentrations as high as 50 percent

organic solvent, chitosan still works as a viscosifier causing the solution to remain

smooth. There are several critical factors affecting chitosan solubility including

temperature and time of deacetylation, alkali concentration, and prior treatments applied

to chitin isolation, ratio of chitin to alkali solution, and particle size.

The solubility, however, is controlled by the degree of deacetylation and it is

estimated that deacetylation must be at least 85% complete in order to achieve the

desired solubility (No et al., 1995). The acid-soluble chitosans with >95% solubility in

1% acetic acid at a 0.5% concentration could be obtained by treatment of the original

chitin with 45-50% NaOH for 10-30 min. Chitosans treated with 45% NaOH for only 5

min, and/or with 40% NaOH for 30 min, were not deacetylated sufficiently to be soluble

in 1% acetic acid. Insoluble particles were found in both solutions. According to Bough

et al. (1978), a reaction time of 5 min with 45% NaOH may not be enough for chitin

13

particles to be sufficiently swollen. A decrease of the NaOH concentration to 40%

required increased time of >30 min to obtain a soluble chitosan (No et al., 2000).

2.2.5 Bulk Density

The bulk density of chitin from shrimp and crab is normally between 0.06 and

0.17 g/ml, respectively (Shahidi and Synowiecki, 1991), indicating that shrimp chitin is

more porous than crab chitin. Krill chitin was found to be 2.6 times more porous than

crab chitin (Anderson et al., 1978). In a study conducted by Rout (2001), the bulk

density of chitin and chitosan from crawfish shell, is very high (0.39 g/cm3). This

perhaps could be due to the porosity of the material before treatment. But once crawfish

shell had been demineralized or deproteinized or both there seem to be very minor

variations unpacked in bulk density between chitin and chitosan produced. A

comparison of the bulk densities of crawfish and commercial chitin and chitosan

indicated some variations, which can be attributed to crustacean species or sources of

chitosan and the methods of preparation (Rout, 2001), as also stated earlier by Brine and

Austin (1981). Rout (2001) reported that increased degree of deacetylation decreased

bulk density.

2.2.6 Color

The pigment in the crustacean shells forms complexes with chitin (4-keto and

three 4, 4'-diketo-ß-carotene derivatives) (Rout, 2001). Chitosan powder is quite flabby

14

in nature and its color varies from pale yellow to white whereas starch and cellulose

powder have smooth texture and white color.

2.2.7 Water Binding Capacity (WBC) and Fat Binding Capacity (FBC)

Water uptake of chitosan was significantly greater than that of cellulose and even

chitin (Knorr, 1982). Basically, WBC for chitosan ranges between 581 to 1150% with an

average of 702%, according to Rout (2001). In his report, Rout (2001) also noted that

reversing the sequence of steps such as demineralization and deproteinization had a

pronounced effect on WBC and FBC. Deproteinization of demineralized shell also gives

higher WBC compared to the process when demineralization of the deproteinized shell

is conducted. Besides, the process of decoloration also causes a decrease in WBC of

chitosan than those of unbleached crawfish chitosan.

The fat uptake of chitin and chitosan ranges from 315 to 170% with chitosan

having the lowest and chitin the highest fat uptake (Knorr, 1982). In a study by Rout

(2001) on this aspect, he reported that the average FBC of crawfish chitosans and

commercial crab chitosans for soybean oil was 706% and 587%, respectively. The

inclusion of decoloration step during the production of chitosan was found to decrease

the fat binding capacity of crawfish chitosans, affect the viscosity of chitosan (Moorjani,

1975). The decreased viscosity as evidenced may be a cause for decrease in fat binding

capacity among unbleached and bleached crawfish chitosan samples.

Rout (2001) also reported that changing the sequence of steps, i.e., when

demineralization is conducted prior to deproteinization, followed by deacetylation,

15

caused an increase in FBC compared with when deproteinization is performed prior to

demineralization, followed by deacetylation.

2.2.8 Emulsification

Even though chitosan alone does not produce emulsions, Cho et al. (1998)

reported that emulsifying capacity of egg yolk (protein) increased with the addition of

chitosan compared with the control. At 0.5% chitosan concentration, better emulsifying

capacity was observed compared with at 0.1 or 0.3% chitosan. In general, chitosan

emulsions tend to be very stable under temperature changes and aging. With viscosity,

the degree of deacetylation is reported to be a determining factor in the emulsification

properties of chitosan. The protein solution containing chitosan with intermediate degree

of deacetylation produces less effective emulsion compared with that containing

chitosan with higher DDA.

2.2.9 Antimicrobial Properties

Recent studies in antibacterial activity of chitosan have revealed that chitosan is

effective in inhibiting growth of bacteria. The antimicrobial properties of chitosan

depend on its molecular weight and the type of bacterium. Gram-positive bacteria retain

crystal violet dye after iodine fixation and alcohol decolorization, whereas gram-

negative bacteria do not. Gram-negative bacteria have an additional outer membrane

containing lipopolysaccharide (endotoxin). For gram-positive bacteria, chitosan with

16

470 KDa was the most effective, except for Lactbacillus sp., whereas for gram-negative

bacteria, chitosan with 1,106 KDa was effective. Chitosan generally showed stronger

bactericidal effects for grampositive bacteria (Listeria monocytogenes, Bacillus

megaterium, B. cereus, Staphylococcus aureus, Lactobacillus plantarum, L. brevis, and

L. bulgaris) than for gram-negative bacteria (E.coli, Pseudomonas fluorescens,

Salmonella typhymurium, and Vibrio parahaemolyticus) in the presence of 0.1%

chitosan (No et al., 2002).

Koide (1998) reported that chitin and chitosan in vitro show antibacterial and

anti-yeast activities. One of chitosan derivatives, N-carboxybutyl chitosan, was tested

against 298 cultures of different pathogenic microorganisms that showed bacteriostatic

and bactericidal activities, and there were marked morphological alterations in treated

microorganisms when examined by electron microscopy (Muzzarelli, 1990).

Conversely, growth inhibition and inactivation of mould and yeasts seem to depend on

chitosan concentration, pH, and temperature (Rout, 2001). According to Cuero (1999),

the antimicrobial action of chitosan is influenced by intrinsic and extrinsic factors such

as the type of chitosan (e.g., plain or derivative), degree of chitosan polymerization, host

nutrient constituency, substrate chemical and/ or nutrient composition, and

environmental conditions such as substrate water activity.

In an extensive research by Tsai and Su (1999) on the antimicrobial activity of

chitosan prepared from shrimp against Ecoli, they found that higher temperature and

acidic pH of foods increased the bactericidal effect of chitosan. They also explained the

mechanism of chitosan antibacterial action involving a cross-linkage between

polycations of chitosan and the anions on the bacterial surface that changes membrane

permeability. Chitosan has been approved as a food additive in Korea and Japan since

1995 and 1983, respectively (KFDA, 1995). Higher antibacterial activity of chitosan at

lower pH suggests that addition of chitosan to acidic foods will enhance its effectiveness

as a natural preservative (No et al., 2002).

17

2.2.10 Formation of Film

Chitosan coating have been shown to significantly delay fruit spoilage or

decaying of fruits and vegetables such as tomatoes, strawberries, etc., at different

temperatures. Chitosan coated fruits were not only firmer and higher in titratable acidity,

but were slow to decay and exhibited less pigmentation than control samples at the end

of storage (El Ghaouth et al., 1992). The low molecular weight chitosan has a greater

inhibitory effect against phytopathogens than the high molecular weight chitosan

(Hirano et al., 1989).

Chitosan has an ability to form film which makes it suitable for use as food

preservation for control of psychotropic pathogen in fresh or processed meat and fish

products packaged under modified atmosphere (Smith et al., 1994). According to

Charles et al. (1994), the most potential application of chitosan is as a coating agent in

the area of fruit preservation. The biodegradability of chitosan is one of the most

advantageous features for concern of the environmental damage occurring by improper

disposal of petrochemical based plastics (Knorr, 1991).

N, O-carboxymethyl chitosan can form a strong film that is selectively

permeable to such gases as oxygen and carbon dioxide. Apples coated with this material

remain fresh for up to six months. The chitosan coating has been shown to delay

ripening of banana for up to 30 days where as chitosan film manifests a slightly yellow

appearance, with the color darkening as thickness increased (Setha et al., 2000).

18

2.3 Production of Chitin and Chitosan

Chitosan is easily obtained from crab especially Dungeness crab (Cancer

magister), shrimp particularly the Pacific shrimp (Pandalus borealis), lobster, or

crawfish shells. These are the richest source of chitin and the major sources of

crustaceans that are processed into chitin and chitosan (Knorr, 1991). While much

research has been done with chitosan extraction from crab shell, limited information is

available on the extraction possibilities with crawfish shell waste.

Previous studies demonstrated that crawfish and crustacean wastes, as well as

organically-rich shellfish processing streams in general, can no longer be considered as

disposable “waste” products with minimal economic value, but should be considered as

profitable alternatives leading to valuable products of commerce (No et al., 1992).

Similar research studies by Lee (1989) demonstrated that the astaxanthin-rich shell from

crawfish waste is a valuable natural resource for commercially feasible pigment which is

marketed as a fish food additive in aquaculture, especially for Salmon.

Apart from the recoverable pigment, it has been shown that crawfish shell waste

possesses a significant and renewable major resource for the biopolymer chitin (23.5%

on a dry basis compared to 14-27% and 13-15% of the dry weight of shrimp and crab

processing waste, respectively) and chitosan (No and Meyers, 1989,1992). Therefore,

the applications of crawfish shell wastes as a source of astaxanthin, chitin and chitosan

represent a total byproduct utilization concept with realistic implications in other

crustacean waste recovery industries (No and Meyers, 1989). Further significance can be

seen in the utilization of astaxanthin pigment, chitin, and protein from crawfish shell as

mentioned earlier in a variety of fields with different applications.

19

Chitin was obtained in pilot scale, according to the procedure of Soares, Moura,

Vasconcelos, Rizzi, and Pinto (2003), through the stages of demineralization, that

consists of the reduction of raw material’s ashes; deproteinization, where there is a

reduction of shrimp wastes’ protein nitrogen; and deodorization, for the reduction of

shrimp’s characteristic odor. Chitin was dried in a tray drier until reaching commercial

moisture content (5.0–6.0%, wet basis).

2.3.1 Isolation of Chitin

Isolation of chitin from crawfish shell wastes involves four traditional steps:

demineralization, deproteinization, decolorization, and deacetylation. However, the

isolation of chitin specifically consists of only two steps: demineralization and

deproteinization, which involves the dissolution of calcium carbonate with 1.0 N HCl

and the removal of proteins with 3% NaOH, respectively.

20

Figure 2.4 Isolation of chitin.

From Shrimp Shells Waste

Step 1 : Demineralization (removing calcium carbonate,phosphate) Step 2 : Deproteinization

Step 3 : Decolorization (removing mainly astaxanthin,pigment)

From Chitin :

Step 1 : Deacetylation (removing acetyl groups from polymer)

We get Chitosan

21

Figure 2.5 Traditional Crawfish Chitosan Production Flow Scheme (Modified from No

and Meyers, 1995)

Wet crawfish shell

Washing and drying

Grinding and sieving

Deproteinization

Washing

Dimineralization

Washing

Decolorization

Washing and drying

Deacetylation

Washing and drying

Chitosan

22

2.3.2 Deproteinization

Chitin occurs naturally in association with protein (chitinoprotein). Some of this

protein can be extracted by mild methods, but other portion is not readily extracted,

suggesting strong covalent bonding to chitin (Attwood and Zola, 1967). With regards to

chemical structure, protein is bound by covalent bonds to the chitin through aspartyl or

histidyl residues, or both, thus forming stable complexes such as glycoproteins.

Crustacean shell waste is usually grounded and treated with dilute sodium hydroxide

solution (1-10%) at elevated temperature (65-100ºC) to dissolve the proteins present.

Reaction time usually ranges from 0.5 to 12 hr depending on preparation methods.

Prolonged alkaline treatment under severe conditions causes depolymerization and

deacetylation. To obtain uniformity in reaction, it is recommended to use relatively high

ratios of solid to alkali solution of 1:10 or 1:15-20 with proper agitation because a

minimum ratio of 1:4 (w/v) of shell weight to potassium hydroxide (KOH) solution, had

only a minor effect on the deproteinization efficiency of shells (No and Meyers, 1995).

2.3.3 Demineralization

The conventional demineralization process of crustacean waste is costly and

causes environmental problems. Hydrochloric acid is the most commonly used chemical

in the demineralization of crustacean waste. The use of this strong acid are to harm the

physiochemical properties of chitin, results in a harmful effluent wastewater, and

increases the cost of chitin purification process. Percot et al. reported that using

hydrochloric acid (HCl) for the demineralization of chitin results in detrimental effects

on the molecular weight and the degree of acetylation that negatively affects the intrinsic

properties of the purified chitin. The authors elaborated on the importance of the

23

optimization of the extraction process parameters (pH, time, temperature and solids to

acid ratio) in order to minimize chitin degradation and bring the impurity levels down to

the satisfactory level for specific applications. Therefore, a less harmful cheaper

demineralization process is needed.

The current study proposes the use of a novel demineralization process in which

organic acids (lactic and acetic) are used. Using organic acids such as lactic and/or acetic

acids for the demineralization process is a Am. J. (2007) promising idea, since organic

acids can be produced from low cost biomass such as cheese whey, are less harmful to

the environment, can preserve the characteristics of the purified chitin, and the resulting

organic salts from the demineralization process can be used as an environmentally

friendly deicing/ anti-icing agents and/or as preservatives.

2.3.4 Decolorization

Acid and alkali treatments alone produce a colored chitin product. For

commercial acceptability, the chitin produced from crustacean sources, needs to be

decolorized which is a process to remove astaxanthins and pigments or bleached to yield

cream white chitin powder (No et al., 1989). The pigment in the crustacean shells forms

complexes with chitin. In earlier research studies, one 4-keto-and three 4, 4’-diketo-

ßcarotene derivatives was firmly bound to the exoskeletal chitin of red kelp crab. The

level of association of chitin and pigments varies from species to species among

crustacean. Several workers have used reagents to eliminate pigments from crustacean

exoskeleton, usually crab.

24

However, with crawfish shell the reagents alone do not seem as effective as the

procedure developed currently. This suggests that carotenoids, are more strongly bound

to the crawfish shell matrix than are those reported from other crustacea (No et al.,

1989). Hence, the stronger the bond the more harsh treatment is required to prepare a

white colored chitin. During the process of decoloration, it should be noted that the

chemical used should not affect the physicochemical or functional properties of chitin

and chitosan. No et al. (1989) was able to prepare a near white colored crawfish chitin

by extraction with acetone and dried for 2 hr at ambient temperature, followed by

bleaching with 0.315 % (v/v) sodium hypochloride solution (containing 5.25% available

chlorine) for 5 min with a solid to solvent ratio of 1:10 (w/v), based on dry shell. But,

the color of chitin products varied from cream white to intermediate pink color (No et

al., 1989). Without prior acetone extraction, bleaching for more than 1 hr was needed to

obtain a commercially acceptable white product.

2.3.5 Deacetylation

The major procedure for obtaining chitosan is based on the alkaline deacetylation

of chitin with strong alkaline solution. Deacetylation is the process to convert chitin to

chitosan by removal of acetyl group. It is generally achieved by treatment with

concentrated sodium or potassium hydroxide solution (40-50%) usually at 100ºC or

higher for 30 min or longer to remove some or all of the acetyl groups from the polymer

(No and Meyers, 1989). The N-acetyl groups cannot be removed by acidic reagents

without hydrolysis of the polysaccharide, thus, alkaline methods must be employed for

N-deacetylation (Muzzarelli, 1977).

25

Kurita (2001) has indicated that deacetylation of chitin can be highly facilitated

by steeping in strong sodium hydroxide solution at room temperature before heating.

This approach was then adapted and the effect of steeping time on the feasibility of

deacetylation was investigated. There are several critical factors that affect the extent of

deacetylation including temperature and time of deacetylation, alkali concentration, prior

treatments applied to chitin isolation, atmosphere (air or nitrogen), ratio of chitin to

alkali solution, density of the chitin, and particle size (Rigby, 1936). Considering all

these as necessary conditions, the ideal purpose of deacetylation is to prepare a chitosan

that is not degraded and is soluble in dilute acetic acid in minimal time.

2.4 Factors Affecting Production of Chitosan

A number of processing factors affect chitosan's physicochemical characteristics

such as temperature of deacetylation, time of deacetylation and alkali concentration,

effect of treatment conditions applied in chitin isolation, atmosphere, ratio of chitin to

alkali solution, and particle size.

2.4.1 Temperature of Deacetylation

Higher temperature tends to increase the degree of deacetylation but reduces

molecular size (Lusena and Rose, 1953). There is a substantially linear relationship

between temperature (plotted along the abscissa as 1/T in K) and the rate of

deacetylation (plotted logarithmically along the ordinate) (Peniston and Johnson, 1980).

26

2.4.2 Time of Deacetylation and Alkali Concentration

Wu and Bough (1978) suggested that deacetylation proceeds rapidly to about

68% during the first 1hr in 50% NaOH solution at 100oC. However, the reaction

progresses gradually thereafter reaching about 78% in 5 hr. Thus, alkali treatment

beyond 2 hr does not deacetylate chitin significantly; rather it degrades the molecular

chain. In a concentration study with 35, 40, and 50% NaOH (Bough et al., 1978), as

alkali concentration decreased, rates of decrease in both viscosity and molecular weight

distribution also slowed. Bough et al. (1978) alluded that chitosan deacetylated for 5 min

with 50% NaOH at 145-150oC had higher viscosities (1.7-16.4 fold) and molecular

weight (1.1-1.8 fold) than did chitosans deacetylated for 15 min. Similarly, decrease in

viscosity with increased reaction time was shown and confirmed.

2.4.3 Effect of Treatment Conditions Applied in Chitin Isolation

Treatment conditions applied to chitosan isolation primarily affect viscosity of

the product than any other property. The use of HCl at concentrations above 1.25 N

adversely affected the viscosity of the final chitosan product. In addition, chitosan

viscosity tends to decrease with increased time of demineralization (Moorjani et al.,

1975). On the other hand, Bough et al. (1978) found that deproteinization with 3%

NaOH, and elimination of the demineralization step in chitin preparation, decreased the

viscosities of chitosan samples, where as Moorjani et al. (1975) indicated that it is not

desirable to bleach the material at any stage since bleaching considerably reduces the

viscosity of the final chitosan product.

27

2.4.4 Atmosphere, Ratio of Chitin to Alkali Solution, and Particle Size.

Many scientists have agreed that free access of oxygen to chitin during

deacetylation has a substantial degrading effect on chitosan. Deacetylation in the

presence of nitrogen yielded chitosan of higher viscosity and molecular weight

distributions than did in air. However, little differences in nitrogen and ash compositions

were observed (Bough et al., 1978). Moorjani et al. (1978) emphasized that the ratio of

chitin solids to alkali solution plays a significant role in determining the quality of

chitosan, based on viscosity determination.

Particle size in chitosan productions has sparked controversial reports on its

effect on chitosan quality. Some agree that small particle size is better than large particle

size. According to Bough et al. (1978), smaller particle size (1mm) results in a chitosan

product of both higher viscosity and molecular weight than that of larger particle size

(above 2 to 6.4 mm). The larger particle sizes require longer swelling time resulting in a

slower deacetylation rate. However, Lusena and Rose (1953) indicated that the size of

chitin particle within the 20-80 mesh range (0.841-0.177 mm) had no effect on the

extent of deacetylation and viscosity of the chitosan solutions.

2.5 Determination of Deacetylation Degree

Dodane and Vilivalam (1998) reported that the degree of deacetylation is one of

the more important chemical characteristics, which could influence the performance of

chitosan in many of its applications (Kofuji et al. 2004). In addition, the degree of

deacetylation, which determines the content of free amino groups in the polysaccharides,

28

can be employed to differentiate between chitin and chitosan. For instance, chitin with a

degree of deacetylation of 75% or above is generally known as chitosan (Brine and

Austin, 1981). The process of deacetylation involves the removal of acetyl groups from

the molecular chain of chitin, leaving behind a complete amino group (-NH2) and

chitosan versatility depends mainly on this high degree chemical reactive amino groups.

There are methods available to increase or decrease the degree of deacetylation. For

example, increase either in temperature or strength of sodium hydroxide solution could

enhance the removal of acetyl groups from chitin, resulting in a range of chitosan

molecules with different properties and hence its applications (Kofuji and Shibata,

1999). Since the degree of deacetylation depended mainly on the method of purification

and reaction conditions (Khor and Lim, 2003; Brine and Austin, 1981), it is therefore

essential to characterize chitosan by determining its degree of deacetylation prior to its

utilization at the developmental stage of drug delivery systems.

Various methods have been reported for the determination of the degree of

deacetylation of chitosan. The methods that have been reported by Kofuji (1999)

included ninhydrin test, linear potentiometric titration, near-infrared spectroscopy,

nuclear magnetic resonance spectroscopy, hydrogen bromide titrimetry, infrared

spectroscopy, and Hirano et al. (1989) reported on first derivative UV-

spectrophotometry. Some of the methods are either too tedious, costly for routine

analysis (nuclear magnetic resonance spectroscopy), or destructive to the sample

(ninhydrin test). Furthermore, many limit the range of degree of deacetylation to which

they are applicable. Lately, the first derivative UV-spectrophotometry was advocated for

the degree of deacetylation determination. The degree of deacetylation values of

chitosan appeared to be highly associated with the analytical methods employed.

29

2.6 Application of Chitosan

Chitin has long been viewed as the nature’s second most abundant polymer. This

is for the fact that it is found not only in shellfish, but also found in insect shells and

fungi cell walls. Chitosan, a refined form of chitin, is prepared by removing the shells

from shellfish. The shells are then ground into a pulverous powder, which is

deacetylated or stripped of specific chemical groups allowing the compound to actively

soak up fats. This aspect of it being able to absorb fat is the main feature which makes it

effective in helping weight loss.

2.6.1 Dietary Supplements

It is effectively a fantastic fat inhibitor which work wonders for those in search

of a safe way to lose body fat. It was universally accepted not as a nutritional

supplement in many past cultures, but rather as a potent water detoxifier and was used in

the depletion and eradication of certain waterborne illnesses and toxic substances often

found in municipal sources. Scientific advances in clinical research have proved that

upon ingestion, chitosan is well tolerated and acts as a cellulose-like fiber within the

human body. This finding proved influential for a myriad of health concerns including;

a potent aid in the weight loss of adults, wound healing, and disease prevention. It

provides much of the fiber that is one of the keys to a healthy, cancer-free diet.

There are a number of functions or uses linked to chitosan. Because of these

applications, chitosan is now marketed as a dietary supplement and is used to thicken

foods, paints and makeup (Rout, 2001). Studies in Japan, the U.S. and Europe have

30

shown that chitin and its derivatives help the digestive process and promote the growth

of beneficial intestinal bacteria necessary for proper digestion and excretion of wastes.

These studies have shown chitin to be helpful in better bowel health and the prevention

of tumors or intestinal polyps that are often the precursor to cancers of the bowel and

colon. Chitin has also been shown to have anti-gastritis, anti-toxin, anti-diarrheal, and

anti-constipation properties.

2.6.2 Industrial Wastewater Treatment

In industrial wastewater treatment, chitosan carries a partial positive charge and

binds to metal ions, thus makes the metal ions removal from waste streams or

contamination sites easier (Asano, 1978). In terms of utilization, crawfish chitosan as a

coagulant for recovery of organic compounds in wastewater was demonstrated to be

equivalent or superior to, the commercial chitosans from shrimp and crab waste shell

and synthetic polyelectrolytes in turbidity reduction (No and Meyers, 1992). The

wastewater released from food processing plants typically seafood, dairy or meat

processing industries contains appreciable amount of protein which can be recovered

with the use of chitosan; this protein, after drying and sterilization, makes a great source

of feed additives for farm animals (Rout, 2001).

31

2.6.3 Medical Applications

Chitin has strong anti-bacterial, anti-fungal and anti-viral properties that make it

extremely useful in medical applications such as bandages, wound dressings, surgical

sutures, periodontal treatments, and cataract surgery (Kratz and Arnander, 1997).

Extensive research has shown chitin and its derivative chitosan to be non-toxic and non-

allergenic. Asano et al. (1978) found that chitin is fully biodegradable and therefore

environmentally friendly.

Chitin and chitosan have been extensively examined and tested by researchers

world-wide in a wide range of medical applications, food and nutrition uses, cosmetics,

beauty aids and other new discoveries. Today, mainly in the U.S. and Japan, more than

two million people take chitin and chitosan as dietary supplements.

Table 2.1 Applications of Chitosan

Industry Application

Wastewater Treatment Removal of metal ions, flocculant/coagulant, protein,

dye, amino acids

Food Industry Removal of dye, suspended solids, preservative, color

stabilization, food stabilizer, thickener and gelling agent,

animal feed additive, etc.

Medical Wound and bone healing, blood cholesterol control, skin

burn, contact lens, surgical sutures, dental plaque

inhibition, clotting agent, etc.

32

Agriculture Seed coating, fertilizer, controlled agrochemical release

Cosmetics Moisturizer, face, hand, and body creams, bath lotion, etc.

Biotechnology Enzyme immobilization, protein separation, cell recovery,

chromatography

Chitin has become known as the miracle fiber. Extensive research has

demonstrated it has no known adverse side effects. Japanese medical doctors have been

recommending for at least 20 years that their patients consume large quantities of chitin.

Much of this enthusiasm is unrelated to weight loss. In Japan medical doctors

recommend chitin as a means of helping cure such conditions as allergies, high blood

pressure, elevated blood fats, arthritis and other problems. In Japan chitin is a food

supplement used in place of dangerous prescription drugs.

CHAPTER 3

MATERIALS AND METHODS

3.1 Isolation of Chitin

Chitin was isolated from shrimp shells that were obtained from local wet market

in Kuantan, Pahang. The raw materials were obtained in solid form from the shrimp

shells, washed with water, dried in oven at 60oC for 24 hours, and was blended into

small pieces (nearly powdered).

3.2 Purification Process

The purpose of purification process is to get rid of the impurities in chitin. It

consists of four steps; deproteinization, demineralization, decolorization, and

deacetylation. Of all these process, the deacetylation process is the important part in this

research for it will determine the effectiveness of chitosan in many of its application by

its degree. The steps of these processes are shown in Figure 3.1 below:

34

Figure 3.1 Steps of purification process

3.2.1 Deproteinization

Dried shrimp shells was deproteinized with 3.5% (w/w) NaOH solution for 2

hours at 65oC with constant stirring inside the incubator shaker at a solid to solvent ratio

of 1:10 (w/v). Samples were then filtered, and filtrate was washed with tap water for 30

minutes and oven-dried.

DECOLORIZATION

DEACETYLATION

DEMINERALIZATION

DEPROTIENIZATION

35

3.2.2 Demineralization

Deproteinized shrimp shells were then demineralized by addition of 1N HCl for

30 minutes at ambient temperature with a solid to solvent ratio of 1:15 (w/v), and then

filtered. The filtrate was washed for 30 minutes with tap water and oven-dried.

3.2.3 Decolorization

After deproteinization process, the shrimp shells were decolorized by addition of

acetone for 10 minutes and dried for 2 hours at ambient temperature, followed by

bleaching with 0.315% (v/v) sodium hypochloride (NaOCl) solution (containing ≈10%

available chlorine) for 5 minutes at ambient temperature with a solid to solvent ratio of

1:10 (w/v) based on dry shell. Samples were then washed with tap water and oven-dried

at 70oC for 24 hours until the powder was crispy.

3.2.4 Deacetylation

Removal of acetyl groups from chitin was achieved by autoclaving where the

parameters such as concentration of sodium hydroxide solution, time of heating, and

temperature of heating were varied. The pressure in autoclave was 30 psia and is fixed.

There are three experiments in deacetylation process where each experiment studied the

parameter that affect the deacetylation degree of chitosan.

36

3.2.4.1 Time of Heating

For the first experiment, removal of acetyl groups from chitin was achieved by

autoclaving for 10, 20 and 30 minutes at 121oC using 50% concentrated sodium

hydroxide solution (NaOH) with a solid to solvent ratio of 1:10 (w/v) according to No et

al. (1989). The resulting chitosans were washed to neutrality in running tap water, rinsed

with distilled water, filtered, and dried at 70oC for 24 hours in the oven and were kept

for analysis.

3.2.4.2 Temperature of Heating

For the second experiment, the pressure for autoclaving is fixed at 30 psia with

temperatures of 98oC, 121oC, and 134oC. The time for heating is chosen from first

experiment with the highest degree of deacetylation. 50% concentrated sodium

hydroxide (NaOH) solution is used with a solid to solvent ratio of 1:10 (w/v). The

resulting chitosans were washed to neutrality in running tap water, rinsed with distilled

water, filtered, and dried at 70oC for 24 hours in the oven.

3.2.4.3 Alkaline (NaOH) Concentration

For the third experiment, the concentration of NaOH is varied; 10%, 30%, 50%,

and 70%. Here, the time for heating is chosen from the first experiment and the

temperature is chosen from the second experiment, both with the highest degree of

deacetylation. The resulting chitosans were washed to neutrality in running tap water,

rinsed with distilled water, filtered, and dried at 70oC for 24 hours in the oven.

37

3.3 Determination of Deacetylation Degree

After each experiment in deacetylation process via autoclaving, each sample was

analyzed for its degree of deacetylation. This was done after drying the resulting

chitosan in oven.

3.3.1 Linear Potentiometric Titration

The determination of deacetylation degree of resulting chitosan is conducted

using linear potentiometric titration. This method was a modification method of Ke and

Chen (1990). Chitosan 0.25g was dissolved in 20 ml of standardised 0.10N HCl (to give

a non-viscous solution) and diluted with 10 ml of distilled water. The pH of the solution

was adjusted to ≈2 with standard 0.01M NaOH and taken as the start point. Under

continuous stirring, 1 ml of standard NaOH (0.5 ml for purified chitosan) was added,

allowed to equilibrate and the pH recorded. This sequence was repeated until the pH

reached a value of 3. The DDA% was calculated from the relation (Broussignac, 1968):

���% �������

��� Equation 3.1

where Q = NDV/m, DV is the volume of NaOH solution between the two inflection

points (in l), N is the concentration of NaOH and m is the dry weight of chitosan (in g).

CHAPTER 4

RESULTS AND DISCUSSIONS

4.1 Results

In this part, all the results of the DDA% analysis are presented in Table 4.1-4.3.

These data were then illustrated in figures, Figure 4.1-4.3. From this figure, it is shown

clearly the effect of each parameter to the DDA% of chitosan.

4.1.1 Time of Heating in Autoclave

Table 4.1 DDA% of different time of heating

Time (min) DDA%

10 89.05

20 85.82

30 82.40

39

Figure 4.1 Effect of time of heating on DDA%

In this experiment, the fixed parameter is the concentration of NaOH, 50%, and

the temperature, 121oC. From Figure 4.1, the highest DDA%, 89.05%, was achieved

within time 10 mins heating in the autoclave. In the other hand, the increasing of time

heating in autoclave decreased the DDA% of chitosan.

78

80

82

84

86

88

90

10 20 30

DD

A (

%)

Time (min)

40

4.1.2 Temperature of Heating in Autoclave

Table 4.2 DDA% of different temperature of heating

Temperature (oC) DDA%

10 80.52

20 89.00

30 98.38

Figure 4.2 Effect of temperature of heating on DDA%

In this experiment, the concentration of NaOH was fixed at 50%, and time

heating at 10 mins. From Figure 4.2, the highest deacetylation degree, 98.38% was

achieved when temperature was 134oC. Higher temperature tends to increase the

deacetylation degree of chitosan.

0

20

40

60

80

100

98 121 134

DD

A (

%)

Temperature (°C)

41

4.1.3 Concentration of Sodium Hydroxide (NaOH)

Table 4.3 DDA% of different NaOH concentration

NaOH Concentration (%) DDA%

10 66.03

30 75.71

50 87.35

70 98.79

Figure 4.3 Effect of NaOH concentration on DDA%

In this experiment, the time of heating and temperature was fixed at 10 mins and

134oC respectively. It was found that the DDA% increase as the concentration of NaOH

0

20

40

60

80

100

10 30 50 70

DD

A (

%)

NaOH concentration (%)

42

increase. From Figure 4.3, the highest deacetylation degree, 98.79% was achieved when

the concentration of NaOH was 70%.

4.2 Discussions

The objective of this research is to enhance the degree of deacetylation of chitin

in chitosan production. Deacetylation was effectively achieved by treatment of chitin

under autoclaving conditions and elevated NaOH concentrations, heating times and

temperature and was evaluated for the preparation of chitosan. Further explanations on

results are discussed in this part.

4.2.1 Time of Heating in Autoclave

According to Wu and Bough (1978), deacetylation proceeds rapidly about 68%

during first 1 hr in 50% NaOH solution at 100oC, and reaching about 78% in 5 hrs when

no pressure applied to the system. In autoclave, the pressure 30 psia was applied during

heating the solution of chitosan. Therefore, the use of the autoclave leads to dramatic

reduction in the time of deacetylation and conservation of energy. The deacetylation

degree of chitosan increased fast at the beginning of reaction process then slowed over

time. It means that after an extent time of reaction, it will decrease the NaOH

concentration in the liquid phase and stopped the deacetylation reaction.

43

4.2.2 Temperature of Heating in Autoclave

Higher temperature tends to increase the degree of deacetylation but reduces

molecular size (Lusena and Rose, 1953). The higher temperature helps to deacetylate

chitosan faster. It was observed that the DDA% of chitosan increased significantly with

the increase of temperature to 134°C. Chitosan production showed significant

differences even with small increments of temperature. A decrease in the temperature to

95°C at standard period (10 minutes) showed a reduction of 9.52% of the deacetylation

of chitosan produced. Higher temperatures were necessary to enable effective

interactions between NaOH and the constituents of the polysaccharides in shrimp shells

thus making it possible to extract higher levels of chitosan.

4.2.3 Concentration of Sodium Hydroxide (NaOH)

In general, alkaline treatment of the deacetylation of chitin proceeds rapidly until

the deacetylation reaches around 75-98%, after which further treatment has only a very

limited effect on the extent of deacetylation unless drastic conditions are used. The most

probable explanation for this condition is that the morphology of chitin chains is such

that the remaining amide groups are inaccessible to the NaOH molecule for alkali

treatment. As it is reported in the literature review, when the alkali concentration is

below 40% there is no deacetylation reaction. In other words, there is not enough driving

force in order to conduct the reaction. It seems that the ability of NaOH diffusion into

chitin was highly diminished and it depends on whether or not it is easy for NaOH to

contact the C (2)-acetamido group in chitosan, which is mainly determined by the crystal

structure and the aggregated state of chitosan. Chitosan with different physical forms

possess different aggregated states and crystal structures. The physical form of chitosan

relies on the specific route for preparing chitosan sample.

44

4.2.4 Limitations of Research

There are some limitations during the experimental progress. These limitations

come from the autoclave that was used for the deacetylation process. The autoclave can

only vary three different time and temperature of heating, and is fixed. This is because

the autoclave is mainly used for sterilization purpose where the time and temperature is

fixed at 121oC and 15mins respectively. This makes it so difficult to vary much more on

time and temperature of heating plus the pressure inside autoclave. The pressure is also

fixed at 30 psia.

CHAPTER 5

CONCLUSION

5.1 Conclusion

The chitin and chitosan have been characterized as to their degree of

deacetylation. The effect of parameters such as temperature of heating, time of heating,

and alkaline concentration to the enhancement of deacetylation degree of chitosan have

been studied and shown. It has been shown that with pressurized condition, the

increasing of temperature of heating in autoclave and the alkaline concentration will

increase the deacetylation degree of chitosan. The highest degree of deacetylation was

achieved at temperature of 134oC and with 70% sodium hydroxide concentration.

Meanwhile, the increasing of time of heating in autoclave turns out to decrease the

deacetylation degree of chitosan production. The first ten minutes of heating shows the

highest degree of deacetylation.

46

5.2 Recommendation

From the literature review, it stated that conventional way of deacetylation is at

atmospheric pressure, and it produces low deacetylation degree of chitosan. So, in this

research, the pressurize condition inside the autoclave helps to enhance the degree of

deacetylation of chitin. Therefore, it is recommended that an equipment that can vary the

pressure is use to run the deacetylation process for it might have affect the degree of

deacetylation of chitosan. In this experiment, the pressure in the autoclave is fixed and

this made it difficult to vary the pressure during the deacetylation process and to see its

effect in the deacetylation degree.

REFERENCES Am J of Biochemistry and Biotechnology 3 (1): 1-9, 2007. Unconventional Approach for

Demineralization of Deproteinized Crustacean Shells for Chitin Production. Anderson, C.G., DePablo, N., and Romo, C.R. 1978. Antarctic Krill (Euphausia

superba) as a source of chitin and chitosan. In Proceeding of the First International Conference on Chitin /Chitosan, R.A.A. Muzarrelli and E.R. Pariser (Ed.), p.54. MIT Sea Grant Program, Cambridge, MA.

Asano, T., Havakawa, N. and Suzuki, T. Chitosan applications in wastewater sludge

treatment. In Proceedings of the First International Conference on Chitin/Chitosan; Muzzarelli, R.A.A. and Pariser. E.R. (Eds.),1978. p. 231-252. MIT Sea Grant Program, Cambridge, MA.

Attwood, N.A., Zola, H. The association between chitin and protein in some chitinous

tissues. Comp. Biochem. Physiol. 1967. 20. p. 993 Berger, J., Reist, M., Chenite, A., Felt-Baeyens, O., Mayer, J. M., & Gurny, R. (2005).

Pseudo-thermosetting chitosan hydrogels for biomedical application. International Journal of Pharmaceutics, 288, 197–206.

Baxter, A., Dillon. M., Taylor. K.D.A. and Roberts. G.A.F., Improved method for i.r.

determination of the degree of N-acetylation of chitosan. Intl J Biol Macromol., 14: 166-169, 1992.

Bough, W.A., Salter, W.L., Wu, A.C.M., and Perkins, B.E. 1978. Influence of

manufacturing variables on the characteristics and effectiveness of chitosan products. 1. Chemical composition, viscosity, and molecular weight distribution of chitosan products. Biotechnol. Bioeng. 20. p.1931.

Brine, C.J. and Austin, P.R. Chitin variability with species and method of

preparation.Comp.Biochem. Physiol. 1981a 69B: 283-286. Broussignac, P., 1968. Chemi-Genrie Chim 99, 1241–1247. Charles, W.L., Admed, E.G., Edo, C., Samir, D., Clauzell, S., John, L., Victor., K and

Joseph, Arul. 1994. Potential of induced resistance to control postharvest diseases of fruits and vegetables. Appalachian Fruit Res. St, 45 Wiltshire Road, Kearneysville, WV 25430.

Chen, R. H., & Tsaih, M. L. (1998). Effect of temperature on the intrinsic viscosity and

conformation of chitosans in dilute HCl solution. International Journal of Biological Macromolecules, 23, 135–141.

48

Cho, Y.I., No, H.K., Meyers, S.P. Physicochemical Characteristics and Functional

Properties of various Commercial Chitin and Chitosan Products. Journal of Agricultural and Food Chemistry. 1998. 46(9). P. 3839-3843.

Cuero, R.G. Antimicrobial action of exogenous chitosan. EXS. 1999. 87.p.315-333. El Gaouth et al., 1992 A. El Gaouth, J. Arul, R. Ponnampalam, R. Castaigne and J. Arul,

Chitosan coating to extend the storage-life of tomatoes, HortScience 27 (1992), pp. 1016–1018.

Dodane V, Vilivalam VD. Pharmaceutical applications of chitosan. Pharm Sci Technol

Today 1998; 1(6):246–53. Green, J.H. and Mattick, J.F. Fishery waste management. In "Food Processing Waste

Management", Green, J.H. and Kramer, A. (Eds.), 1979. p.202-227. AVI PublishingCo.,Westport, CT.

Hirano, S., Nagao, N. Effects of chitosan, pectic acid, lysozyme and chitinase on the

growth of several phytopathogens. Agric. Biol. Chem. 1989. 53. p.3065-3066. H. Ke and Q. Chen, Potentiometric titration of chitosan by linear method. Huaxue

Tongbao 10 (1990), pp. 44–46. Johnson, E.L. and Peniston, Q.P. Utilization of shellfish waste for chitin and chitosan

production. In Chemistry and Biochemistry of Marine Food Products; Martin, R.E., Flick, G.J., Hebard, C.E., Ward, D.R., Eds.; AVI Publishing:Westport, CT. 1982; Chapter 19.

KFDA, 1995. Korea Food and Drug Administration. Food Additive Code. Khan, T., Peh, K., Ch’ng, H.S. Reporting degree of deacetylation values of chitosan: the

influence of analytical methods. J Pharm Pharmaceut Sci. 2002. 5(3):205-212. Khor E, Lim LY. Implantable applications of chitin and chitosan. Biomaterials

2003;24(13):2339–49. Knaul, J., Z., Hudson, S.M., Creber, K.A.M. Journal of Polymer Science:Part B:Polymer

Physics. 1999. 72: 1079-1094. Knorr, D. Functional properties of chitin and chitosan. J. Food Sci. 1982. 47: 593-595. Kofuji K, Akamine H, Qian CJ, Watanabe K, Togan Y, Nishimura M, et al. Therapeutic

efficacy of sustained drug release by chitosan gel on local inflammation. Int J Pharm 2004; 272(1–2):65–78.

49

Kofuji K, Shibata K, Murata Y, Miyamoto E, Kawashima S. Preparation and drug retention of biodegradable chitosan gel beads. Chem Pharm Bull 1999; 47(10):1494–6.

Koide S.S. Chitin-Chitosan: properties, Benefits and Risks. Nutrition Research. 1998.

18(6): 1091-1101. Kurita K. Polymer Degradation and Stability, 1998. 59. p.117-120. Kratz, G., Arnander, C., Swedenborg, J., Back, M., Falk, C., Gouda, I., Larm, O.

Heparin-Chitosan complexes stimulate wound healing in human skin. Scand. J. Plast. Hand Surg. 1997. 31. p.119-123.

Lee, K.S., No, H.K., Meyers, S.P. Effect of Chitosan as a Coagulant on Shelf-life of

Tofu Prepared in Commercial scale. Food Science and Biotechnology. 2001. 10(5). p.529-533.

Li, Q., Dunn, E.T., Grandmaison, E.W. and Goosen, M.F.A. Applications and properties

of chitosan. J. Bioactive and Compatible Polym. 1992. 7: 370-397. Lusena, C.V. and Rose, R.C. Preparation and vicosity of chitosans. J. Fish. Res. Bd. Can.

1953. 10(8): 521-522. Maghami, G.G., Roberts, G.A.F. Mackromol. Chem. 1988. 189. p.195-200. Mima, S., Miya, M., Iwamoto, R., Yoshikawa, S. Highly Deacetylated Chitosan and Its

Properties. Journal of Applied Polymer Sciences. 1983. 28. p.1909-1917. Moorjani M.N., Khasim D. I., Rajalakshmi S., Puttarajappa P., Amla B.L. Chitosan of

high viscosity and protein as a valuable by-product from squilla. In Proceedings of the First International Conference on Chitin/Chitosan; Muzzarelli, R.A.A. and Pariser. E.R. (Eds.), 1978. p.210-216. MIT Sea Grant Program, Cambridge, MA.

Moorjani, M.N., Achutha, V., and Khasim, D.I. Parameterss affecting the viscosity of

chitosan from prawn waste. J. Food Sci. Technol., 1975. 12. p.187-189. Moore, G.K. and Roberts, G.A.F., in Muzzarelli, R.A.A. and Pariser, E.R., (eds),

Proceedings of the first International Conference on chitin/chitosan. MIT Sea Grant Report 78-7, pp 421, 1978.

Muzzarelli, R.A.A. Chitin; Pergamom: Oxford, 1977. Muzzarelli R, Tarsi R, Fillippini o, Giovanetti E, Biagini G, Varaldo P E. Antimicrobial

properties of N-carboxylbutyl chitosan. Antimicro Ag Chemotherap 1990; 34. p.2019- 2023.

50

No, H.K., Meyers, S.P. Preparation and Characterization of Chitin and Chitosan-A Review. Journal of Aquatic Food Product Technology. 1995. 4(2). P.27-52.

No, H.K., Cho,Y.I., Kim, H.R., Meyers, S.P. Effective Deacetylation of Chitin under

Conditions of 15 psi/121ºC. Journal of Agricultural and Food Chemistry. 2000. 48 (6). p.2625-2627.

No, H.K., Lee, K.S., Meyers, S.P. Correlation Between Physicochemical Characteristics

and Binding Capacities of Chitosan Products. Journal of Food Science. 2000.65(7). p.1134-1137.

No, H.K., Meyers, S.P. Crawfish Chitosan as a Coagulant in Recovery of Organic

Compounds from Seafood Processing Streams. J. Agric. Food Chem. 1989. 37(3): 580-583.

Peniston, Q.P. and Johnson, E.L. 1980. Process for the manufacture of chitosan. U.S.

patent 4,195,175. Percot, A., C. Viton and A. Domard, 2003. Optimization of chitin extraction from

shrimp shells. Biomacromolecules, 4:12-18. Ravi Kumar M.N.V., A review of chitin and chitosan applications, Reac. & Func.

Polym., 46,1-27 (2000). Razdan A. and Petterson D., Effect of chitin and chitosan on nutrient digestibility and

plasma lipid concentrations in broiler chicken. Brit J Nutri 72 (1994), pp. 277–288. Rege, P. R., Garmise, R. J., & Block, L. H. (2003). Spray-dried chitinosans. Part I.

Preparation and characterization. International Journal of Pharmaceutics, 252, 41–51.

Riccardo A. and Muzzarelli A., Chitin (metal ion chelation and enzyme

immobilization)., in Polymer Materials Encyclopedia, Joseph C. Salamone., Ed., 2, 1200-1205, CRC press, USA (1996).

Rigby, G.W. 1936. Chemical products and process of preparing the same. U.S. patent

2,072,771. Rout, S. K. Physicochemical, Functional, and Spectroscopic analysis of crawfish chitin

and chitosan as affected by process modification. Dissertation. 2001. Roberts, G. A. F., & Domszy, J. G. (1982). Determination of the viscosimetric constants

for chitosan. International Journal of Biological Macromolecules, 4, 374–377. Setha, S., Kanlayanarat, S., Gemma, H. 2000. Effect of various molecular weight of

chitosan coating on the ripening of caven dish banana. Division of Postharvest Technology, King MongKurt's university, Bangkok, Thailand.

51

Shahidi, F. and Synowiecki, J. Isolation and charactrization of nutrients and value-added

products from snow crab (Chinoecetes opilio) and shrimp (Pandalus borealis) processing discards. J. Agric. Food Chem. 1991 39: 1527-1532.

Smith, J.P. Simpson, B.K. and Morris, J. 1994. Control of psychotropic pathogens in

fresh/ processed meat and fish products packaged under modified atmosphere, Macdonald Campus of McGill university, Faculty of Agriculture and Environmental Science, Quebec, H9X3V9.

Soares, N. M., Moura, C., Vasconcelos, S., Rizzi, J., & Pinto, L. A. A. (2003).

Obtenc¸a˜o e purificac¸a˜o de quitosana a partir de resı´duos de camara˜o em escala piloto. Revista Univap, Sa˜o Jose´ dos Campos, SP, Brasil, 10(18), 88–92.

Tolaimate, A., Desbrieres, J. Rhazi, M. Alagui, A., Vincendon, M., Vottero, P. On the

influence of deacetylation process on the physicochemical characteristics of chitosan from squid chitin. Polymer. 2000. 41. p.2463-2469.

Tsai, G.J. and Su, W.H. Antibacterial activity of shrimp chitosan against Escherichia

coli. J. Food Prot. 1999. 62(3): 239-243. Wu, A.C.M. and Bough, W.A. 1978. A study of variables in the chitosan manufacturing

process in relation to molecular-weight distribution, chemical characteristics and waste treatment effectiveness. In Proceedings of the First International Conference on Chitin/Chitosan; R.A.A. Muzzarelli and E.R. Pariser (Ed.) p.88. MIT Sea Grant Program, Cambridge, MA.

52

APPENDIX A

Calculation of Deacetylation Degree of Chitosan on various times of autoclave.

���% �1 � 161�

1 42�

� ���

�

Where, N = NaOH concentration

DV = volume of NaOH used for titration

m = mass of dry chitosan

For 10 mins autoclaved:

1st titration: m = 0.25g

N = 0.01M

DV = 0.0146mL

� ��0.01��0.0146�

0.25� 5.84 � 10��

���% � 1 � 161�5.84 � 10���

1 42�5.84 � 10���� 100 � 88.43%

2nd titration: m = 0.25g

N = 0.01M

DV = 0.013Ml

� ��0.01��0.013�

0.25� 5.2 � 10��

���% � 1 � 161�5.2 � 10���

1 42�5.2 � 10���� 100 � 89.67%

Average DDA% = 88.43% + 89.67%

= 89.05%

53

For 20mins autoclaved:

1st titration: m = 0.25g

N = 0.01M

DV = 0.019mL

� ��0.01��0.019�

0.25� 7.6 � 10��

���% � 1 � 161�7.6 � 10���

1 42�7.6 � 10���� 100 � 85.05%

2nd titration: m = 0.25g

N = 0.01M

DV = 0.017mL

� ��0.01��0.017�

0.25� 6.8 � 10��

���% � 1 � 161�6.8 � 10���

1 42�6.8 � 10���� 100 � 86.58%

Average DDA% = 85.05% + 86.58%

= 85.82%

For 30mins autoclaved:

1st titration: m = 0.25g

N = 0.01M

DV = 0.023mL

� ��0.01��0.023�

0.25� 9.2 � 10��

54

���% � 1 � 161�9.2 � 10���

1 42�9.2 � 10���� 100 � 82.02%

2nd titration: m = 0.25g

N = 0.01M

DV = 0.022mL

� ��0.01��0.022�

0.25� 8.8 � 10��

���% � 1 � 161�8.8 � 10���

1 42�8.8 � 10���� 100 � 82.77%

Average DDA% = 82.02% + 82.77%

= 82.40%

55

APPENDIX B

Calculation of Deacetylation Degree of Chitosan on various temperatures of autoclave.

For T = 98oC,

1st titration: m = 0.25g

N = 0.01M

DV = 0.023mL

� ��0.01��0.023�

0.25� 9.2 � 10��

���% � 1 � 161�9.2 � 10���

1 42�9.2 � 10���� 100 � 82.02%

2nd titration: m = 0.25g

N = 0.01M

DV = 0.027mL

� ��0.01��0.027�

0.25� 1.08 � 10��

���% � 1 � 161�1.08 � 10���

1 42�1.08 � 10���� 100 � 79.03%

Average DDA% = 82.02% + 79.03%

= 80.52%

For T = 121oC,

1st titration: m = 0.25g

N = 0.01M

DV = 0.0146mL

56

� ��0.01��0.0146�

0.25� 5.84 � 10��

���% � 1 � 161�5.84 � 10���

1 42�5.84 � 10���� 100 � 88.43%

2nd titration: m = 0.25g

N = 0.01M

DV = 0.013mL

� ��0.01��0.013�

0.25� 5.2 � 10��

���% � 1 � 161�5.2 � 10���

1 42�5.2 � 10���� 100 � 89.67%

Average DDA% = 88.43% + 89.67%

= 89.05%

For 134oC,

1st titration: m = 0.25g

N = 0.01M

DV = 0.002mL

� ��0.01��0.002�

0.25� 8.0 � 10��

���% � 1 � 161�8.0 � 10���

1 42�8.0 � 10���� 100 � 98.38%

57

2nd titration: m = 0.25g

N = 0.01M

DV = 0.002mL

� ��0.01��0.002�

0.25� 8.0 � 10��

���% � 1 � 161�8.0 � 10���

1 42�8.0 � 10���� 100 � 98.38%

Average DDA% = 98.38%

58

APPENDIX C

Calculation of Deacetylation Degree of Chitosan on various concentration of NaOH in

autoclave.

For 10% NaOH,

1st titration: m = 0.25g

N = 0.01M

DV = 0.045mL

� ��0.01��0.042�

0.25� 1.8 � 10��

���% � 1 � 161�1.8 � 10���

1 42�1.8 � 10���� 100 � 66.03%

2nd titration: m = 0.25g

N = 0.01M

DV = 0.045mL

� ��0.01��0.042�

0.25� 1.8 � 10��

���% � 1 � 161�1.8 � 10���

1 42�1.8 � 10���� 100 � 66.03%

Average DDA% = 66.03%

59

For 30% NaOH,

1st titration: m = 0.25g

N = 0.01M

DV = 0.030mL

� ��0.01��0.030�

0.25� 1.2 � 10��

���% � 1 � 161�1.2 � 10���

1 42�1.2 � 10���� 100 � 76.81%

2nd titration: m = 0.25g

N = 0.01M

DV = 0.033mL

� ��0.01��0.033�

0.25� 1.32 � 10��

���% � 1 � 161�1.32 � 10���

1 42�1.32 � 10���� 100 � 74.61%

Average DDA% = 76.81% + 74.61%

= 75.71%

For 50% NaOH,

1st titration: m = 0.25g

N = 0.01M

DV = 0.016mL

60

� ��0.01��0.016�

0.25� 6.4 � 10��

���% � 1 � 161�6.4 � 10���

1 42�6.4 � 10���� 100 � 87.35%

2nd titration: m = 0.25g

N = 0.01M

DV = 0.016mL

� ��0.01��0.016�

0.25� 6.4 � 10��

���% � 1 � 161�6.4 � 10���

1 42�6.4 � 10���� 100 � 87.35%

Average DDA% = 87.35%

For 70% NaOH,

1st titration: m = 0.25g

N = 0.01M

DV = 0.002mL

� ��0.01��0.002�

0.25� 8.0 � 10��

���% � 1 � 161�8.0 � 10���

1 42�8.0 � 10���� 100 � 98.38%

61

2nd titration: m = 0.25g

N = 0.01M

DV = 0.001mL

� ��0.01��0.001�

0.25� 4.0 � 10��

���% � 1 � 161�4.0 � 10���

1 42�4.0 � 10���� 100 � 99.19%

Average DDA% = 98.38% + 99.19%

= 98.79%

62

APPENDIX D

Isolation of chitin processes.

i) Drying of shrimp shells ii) Blended shrimp shells

iii) Blended shrimp shells iv) Deproteinization prosess

v) Demineralization process vi) After Decolorization process

-Final Chitin

63

APPENDIX E

Deacetylation process

i) Before autoclaving process ii) After autoclaving process

iii) Filtration after autoclaving iv) Drying in oven after filtration

64

v) Resulting chitosan after deacetylation

vi) Titration process

65

APPENDIX F

Type of Autoclave.

66