Engineering and producing flavor and fragrances based on fermentation

Natural products are vulnerable because of weather conditions

Diseases: Citrus greening

Scarcity

IndiaIndian sandalwood (S. album) is one of the most well-known species of sandalwood. In 1998, this species was recognized as “vulnerable” by the International Union for the Conservation of Nature’s (IUCN) Red List, due to disease, fire and exploitation through illegal activity.

Much of sandalwood’s exploitation resulted from a long-standing government restriction;theIndian government’s monopoly of ownership over the country’s sandalwood trees after its designation of sandalwood as a “royal tree,” gave the government complete ownership over the sandalwood trees’ cultivation and trade

The market for Natural Products is growing

Conventional processes are: Inefficient:160.000 Oranges for 1 kg Valencene High environmental footprint Use pesticides

Distillation

Fermentation

Glucose

Renewable & sustainable

Proprietary fermentation technology (Similar to brewing beer)

Rhodobacter Platforn

& Building blocks

Terpene synthase discovery

Strain Improvement

Rational & Evolutionary

HTS - Identification of improved

strains

Fermentation protocol

development

Fermentation test – pilot fermenter:

Scale-up

(Bio)Catalytic Oxidation & DSP

R&D & production capabilities focused on F&F market

Natural Organoleptic quality Low volumes Attractive pricing Kosher - Halal

Technology platform - Strain

Rhodobacter sphaeroides

No fermentation off odors

Strain - established natural isoprenoid producer

High solvent and isoprenoid tolerance

Can switch between different growth modes: aerobic, anaerobic, photosynthetic

Broad acceptance of different carbon sources: C6- and C5- sugars, organic acids, methanol

Technology platform - Strain

Continuously pathway optimization

protected by patents

Double pathway provides high carbon flux

AcCoA mvaAact hcs mvk pmk mvdMVA IPP idi IspA FPPDMAPP

G-3P

Pyr

DXP

TCA

pyridoxal

thiamine

Valencene

Glucose

Technology platform – Plug in terpene synthases

Plug-in terpene synthases: produce specific terpene or terpene mixture with high yields

Access to unique and highly active synthases

Proprietary synthases for valencene, patchoulol, santalene a.o.

Organoleptic testing in early stage

Technology platform – Fermentation protocol

Representative 1L, 7L, 40L and 200 L scale fermenter systems

Online – and offline measurements of critical parameters

Proven scalability of protocols to production

Specific DSP equipment

Technology platform – Scalable and robust fermentation process

Reliable > 50,000-fold scaling

Consistent performance between development and process scales

=> allows for rapid scale-up

Robust performance at commercial scale

Technology platform – Down stream processing (DSP)

Specific DSP know-how and solutions for high product recovery from

broth

Developed and scaled protocols for distillation

Expertise in low volume at low costs productionFermentation

costsDSP I

costs

DSP II

costs

Isobionics costs

Operations with large production capacity

- Footprint only 1% of the agricultural surface needed

- Only renewable materials

- Pesticides free

Fermentation isTraditional, sustainable and environmentally friendly

Fermentation isTraditional, sustainable and environmentally friendly

Always available: No seasons & large capacity

Constant quality: No reformulations

Natural Valencene 75 and 80 (>75% - 80%)

A clear colorless liquid with a sweet woody orange top note and a typical orange taste.

All Pesticides free

Isobionics is the first company in the world that has succeeded in bringing a natural flavor, “Valencene(80) Pure ™”, based on a proprietary fermentation process to the market

Nootkatone 80 (>80%) a typical fruity grapefruit top note and juicy grapefruit taste.

The appearance is a clear, yellow liquid mix with crystals.

Nootkatone 70 (>70%) a typical woody grapefruit top note and juice grapefruit taste.

The appearance is a clear, yellow liquid.

Nootkatone 95 (>95%) a typical fruity grapefruit top note and juicy grapefruit taste.

The appearance is a clear, yellow liquid mix with crystals.

In compliance with EU legislation

Nootkatone 98 (>98%) has a typical fruity grapefruit top note and juicy grapefruit taste.

The appearance is white crystals.

Nootkatone for fragrance applications.

All Pesticides free

Natural Nootkatone 70 (>70%)

has a typical grapefruit woody top note and grapefruit woody taste.

The appearance is a clear, yellow liquid.

All Pesticides free

Natural Beta Bisabolene (85%)

Pleasant balsamic, green odour

> 85% pure β-Bisabolene (CAS 495-61-4)

Appearance: Clear, colourless liquid

Refractive index (@ 20°C): 1.485 - 1.495

All Pesticides free

Product sample available

Commercial release planned: November 2017

Start Sept ‘16 – Commercial release Nov’17

Patchouli

Sandalwood

Thujopsene

Cubebol

Rotundone

Sclareol

(-)--Bisabolol

-Ionone

Nootkatene

Fragrance Flavor

Humulene