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ENDURO Horizontal Electrophoresis Systems

ENDURO Horizontal Electrophoresis Systems. Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of

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Page 1: ENDURO Horizontal Electrophoresis Systems. Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of

ENDURO Horizontal Electrophoresis Systems

Page 2: ENDURO Horizontal Electrophoresis Systems. Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of

Casting a GelAttach a buffer dam to one side of gel tray

Attach the other buffer dam to the other side of gel tray

Place gel tray on a flat surface

Page 3: ENDURO Horizontal Electrophoresis Systems. Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of

Casting a gel• Mix agarose buffer solution and boil

• Cool agarose solution to below 60ºC

• Pour onto gel tray

• Let solution cool for ½ hour

• Gently remove combs

Page 4: ENDURO Horizontal Electrophoresis Systems. Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of

Running the GelRemove the casting gates

Place the gel tray into the gel box

Overlay it with buffer

Connect the lid & attach to a power supply

Page 5: ENDURO Horizontal Electrophoresis Systems. Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of

Staining the Gel1. Remove gel from gel tray2. Place into a container with stain3. Destain the gel with buffer or water

Page 6: ENDURO Horizontal Electrophoresis Systems. Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of

Features

• Heavy duty molded chambers

• Removable electrodes

• Multichannel pipette compatible combs

• Domed lid to prevent condensation

Page 7: ENDURO Horizontal Electrophoresis Systems. Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of

Heavy duty molded construction• Prevents leaking problems

Page 8: ENDURO Horizontal Electrophoresis Systems. Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of

Changing the electrode

Turn unit over & remove screw

Remove old electrode

Insert new electrode & secure with nylon screw

Page 9: ENDURO Horizontal Electrophoresis Systems. Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of

Multichannel CombsA large number of comb are compatible with multichannel pipettes

Page 10: ENDURO Horizontal Electrophoresis Systems. Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of

Domed LidPrevents condensation from falling on the gelRecaptures condensation in reservoirs