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26 Free Papers—Oral Presentations

wo ACC cell lines, ACC-2 and ACC-M,ere chosen to assess the cell proliferation,

he cell invasion, the cell cycle and apopto-is change after BMP-2 treatment, by MTT,ranswell Matrigel invasion assay, flowytometry (FCM), and Bcl-2 was examinedy immunohistochemical staining as well.he level of phosphorylated extracellularignal-regulated kinase 1 and 2 (ERK1/2)as detected by Western bolt analysis.urthermore, ACC-M cells, mixed withMP-2 or not in Matrigel, were subcu-

aneous injected into mice respectively.umour volume measurements began onay 7 (tumour formation) and continuedhree times a week until the end of thistudy.esults: BMP-2 was overexpressed in theytoplasm of ACCs, and the expressionatio of BMP-2 in the solid subtype ofCCs group was higher than that ofribriform-tubular subtype, but the expres-ion ratio of BMP-2 had no correlation withhe tumour size, patient gender and age.n vitro study, BMP-2 stimulated prolifer-tion of ACC-2 and ACC-M cells. Thoughhere was no significant change on the ratiof G0/G1 phase, S phase, or G2/M phase,cl-2 expression was significantly upregu-

ated and invasion activity of the cells wasignificantly enhanced after BMP-2 treat-ent. Western blot showed the p-ERK1/2

evel of ACC-2 and ACC-M cell lines wasemarkably upregulated 10 minutes afterMP-2 treatment. In vivo there was sig-ificant increase of the tumour volume atarly stage with BMP-2 treatment.onclusion: BMP-2 can be overexpressed

n ACCs, and might have adverse biologi-al effects on tumourigenesis of ACCs.

oi:10.1016/j.ijom.2009.03.107

1.4easible technique for therediction of lymph node

nvolvement in squamous cellarcinoma of the tongue byuclear morphometric analysis. Karino ∗, H. Ishibashi, S. Fujihara,. Watanabe, Y. Ichiba, Y. Nariai,

. Kondo, J. SekineDepartment of Oral and Maxillofacialurgery, Shimane University Faculty ofedicine, Izumo, Japan

ackground and Objectives: An accuratevaluation of cervical lymph node metas-

ases is crucial for the correct managementf oral cancer. A number of studies haveeported the predictive factor for cervicalymph node metastases in oral cancer.his study was undertaken to confirm

the feasibility of nuclear morphometricanalysis of lymph node metastases ofsquamous cell carcinoma in the tongue.Methods: Forty nine cases of squamouscell carcinoma (25 cases with metastasisedlymph nodes, 24 node-negative cases) wereexamined. Using computerised image sys-tem, the mean nuclear area, the nuclearperimeter, the circular rate, the largest tothe smallest dimension ratio (LS ratio) ofthe nuclei, and the coefficient of variationof the nuclear area (NACV) were measuredin the biopsy specimens. The relationshipbetween these results and the lymph nodeinvolved has evaluated retrospectively.Results: The nuclear area and perimeterwere significantly higher in cases withmetastasised lymph node than in node-negative cases (P < 0.05). On the otherhand, the circular rate was slightly smallerin cases with metastasised lymph node thanin node-negative cases. The LS ratio andNACV were slightly higher in cases withmetastasised lymph node than in node-negative cases. In lymph node positivecases, the level of lymph node metastaseswas in inverse proportion to circular rate.And significant difference was not foundin other parameters.Conclusions: Preoperative quantitativeestimations of nuclear features could pro-vide a feasible criterion for the predictionof lymph node metastases in squamous cellcarcinoma of the tongue.

doi:10.1016/j.ijom.2009.03.108

O1.5Efficiency of serum tumourmarkers oral squamous cellcarcinoma patientsB.J. Kim ∗, D.Y. Bhang, C.W. KimDepartment of Oral and MaxillofacialSurgery, Dankook Dental Hospital,Dongnam-gu, Cheonan-si, Chungnam,Korea

Background and Objectives: Recently, therole of serum tumour marker has been stud-ied for an important issue on diagnosingand treating tumours in the head and neckregion because tests using tumour markersneed relatively simple procedures and areacceptable to patients, compared with othertest methods. Tumour marker tests wereperformed on patients with squamous cellcarcinoma, which were known to have thehighest prevalence among tumours in the

head and neck region. Association betweeneach tumour marker, and diagnosis andprognosis of tumours was assessed.Methods: Tumour marker tests were car-ried out on 31 patients who visited Oral

and Maxillofacial Surgery Departmentin Dankook University Dental Hospi-tal between January 2003 and August2008 and who were diagnosed as primaryoral squamous cell carcinoma throughouthistopathologic diagnosis. Blood samplesfrom these patients were analysed tomeasure tumour markers using nuclearmedicine diagnostic equipment. Measuredentries were as follows: PSA (prostate-specific antibody), SCC-Ag (squamouscell carcinoma related antigen), CA19-9 (cancer antigen 19-9), ferritin,�-FP (alpha-fetoprotein), CYFRA 21-1(cytokeratin-19 fragments), CA125 (can-cer antigen 125) and p53.Results: Analyses on each tumour markerindicated that squamous cell carcinomain the head and neck region had statis-tically significant correlation with p53,SCC-Ag (TA-4), CYFRA 21-1 and ferritin.p53 demonstrated the highest sensitivity.Especially, 4 cases among 18 cases whichFerritin was measured exhibited metasta-sis. In all those 4 cases, Ferritin values werehigher than the standards (15–332 ng/mL).Therefore, ferritin is considered to have aclose relation with metastasis of squamouscell carcinoma.Conclusions: This study shows that tumourmarker tests are more useful in evaluat-ing progression and prognosis of tumoursrather than in diagnosing them. Particu-larly, serum Ferritin is considered to bebeneficial in assessing metastasis of squa-mous cell carcinoma in the head and neckregion and in developing treatment plansbased on the assessment.

doi:10.1016/j.ijom.2009.03.109

O1.6Whole-body diffusion weightedimaging manifestation of oralmalignancy with metastaticlymph nodesR. Yan ∗, X. Gu, G. YangCenter of Stomatology, The GeneralHospital of the Chinese People’s ArmedPolice Forces, Hai Dian District, Beijing,China

Background and Objectives: Diffusion-weighted magnetic resonance imaging(DWI) provides functional informationand can be used for the detection andcharacterisation of pathologic processes,including malignant tumours. The recently

introduced concept of whole body dif-fusion weighted imaging (WB-DWI)with background body signal suppressionnow allows acquisition of volumetricdiffusion-weighted images of the entire