EFFECTS OF ACUTE EXERCISE ON SIGNAL TRANSDUCER AND ACTIVATOR OF
TRANSCRIPTION 3 & CHEMOKINE(C-X-C MOTIF)
RECEPTOR 4 GENE Gerry Woodland1, Kelsey Corrigan2, Rian Q
Landers2, James M Hagberg2
1. Department of Science, Fayetteville State University, NC2.
Department of Kinesiology, School of Public Health, University of
Maryland College Park
The purpose of this study is to assessed the effect of acute
exercise onPeripheral Blood Monocular cells (PBMCs) in gene
expression for STAT3and CXCR4.
There were no significant different seen in any of the genes
after acute bout of exercise. These mean that these genes are not
undergoing physiological difference showing acute bout ofexercise.
A study with increase of more sample size and different race will
make the study more significant A sample size with more
participants will make the study more significant. It will also be
useful to test on JAK2 with other genes to access the effect of
acute exercise on PBMCs. Since STAT3 is in the JAK/STAT pathway, it
could show up with an interesting result. A future direction for
this research is to test the effect of acute exercise on athlete
with a history of cardiovascular disease and to complete the bout
of exercise on both the treadmill andcycling ergometer.
• Regular exercise and physical activities can help the body
systemfunction better and decrease the risk of cardiovascular
disease.
• It has been shown that STAT3 is expressed in the heart and
culturedcardiac myocytes and endothelial cells
• STAT3 is involved in the regulation of inflammatory, cell
growth, cellmovements and self-distruction of cells.
• STAT3 is mostly linked to the reduction of inflammation.• It
has also been shown that STAT3 plays a significant role in
protecting
the cardiac cells from different kind of stress.• CXCR4 is a
chemokine(C-X-C motif) receptor 4, located in the
nucleus.• CXCR4 has been shown to regulate vascular branching
and remolding
process in endothelial cells.
Chapter 2: Physical Activity Has Many Health Benefits. (n.d.).
Retrieved from Pyscial Activity Guidelines:
http://www.health.gov/paguidelines/guidelines/chapter2.aspxGenetic
Home Reference . (2014, July 28th). Retrieved from Genes:STAT3:
http://ghr.nlm.nih.gov/gene/STAT3Verma, S. K., & Kishore, R.
(2012). Roles of STATs signaling in cardiovascular disease.
JAK/STAT Signaling in the mycardium , 1-7.WH, X., J, L., C, S., Z,
Y., L, C., F, W., . . . J, T. (2011). Physical exercise attenuates
age-associated reduction in endothelium-reparative capacity of
endothelial progenitor cells by increasing CXCR4/JAK-2 signaling
in
healthy men. PubMed, 111-119.
Subjects - Participants (n=11) were 18 - 23 years old with no
existing medical history of CVD. Written informed consent form was
provided by the participants and the project was approved by the
University of Maryland IRB. Baseline Testing-Composition of body
mass was determined in a Bod Pod device, which requires the
participant to sit quietly in an air-tight chamber during which the
volume of their body is measured. Participants then performed Vo2
max test on the treadmill ergometer. They had a face mask covering
their nose and mouth to measure oxygen consumption. Their heart
rate was monitored during the exercise. They started running at a
constant rate and incline increased 2% every two minutes until the
subject reached exhaustion or multiple criteria of Vo2 max was
reached. The criteria used to determine if a true maximum was
elicited were: respiratory exchange ratio (RER) >1.1, rating of
perceived exhaustion (RPE) > 18, peak heart rate within 10 bpm
of their age predicted maximum.Acute Exercise Test –10mL of blood
sample were taken from the participants after an overnight 12hr
fast which was drawn from their anticubital veins by a trained
researcher. The resting heart rate was taken and participant run
30min continuously on a treadmill at a speed and incline selected
to 70% of their true VO2Max. Their heart rate were monitored during
the test and they breathed into the face mask every 5min to record
their VO2 max. After the conclusion of the test, 10mL of blood
sample were drawn from the participant.Peripheral Blood Mononuclear
Cells (PBMCs) Isolation - the 10ml of blood obtained from the
subjects was diluted with 1x phosphate-buffered saline (PBS) and
then placed onto the surface of Ficoll which helped the blood
separate into plasma, PBMCs, Ficoll-paque and erythrocytes from top
to bottom respectively. These cells were mixed with PBS + 2% Fetal
Bovine Serum (FBS) Lastly, trizol was added to lyse the cells, then
samples were placed in a -80C freezer until use. RNA was isolated
from these cells and reversed transcribed to cDNA. Using specific
primers for STAT3 and CXCR4 genes, PCR was carried out and the
product was run at 1.5% agarose gel.
Max Exercise Test Variables
Minimum Maximum Mean
Statistics Statistics Statistic Std. deviationAge(ys) 18 25
22.18 1.68Height(in) 62 72.2 67.28 3.57Weight (Ibs) 113 225 152.84
26.74VO2 Max absolute(mL/min)
2605 4614 3638.09 720.91
VO2 Max Relative(mL/min)
41.9 39.2 50.64 8.86
Max Respiratory Exchange Ratio (RER)
1.03 1.20 1.12 0.0481
Max Heart Rate(bpm) 177 207 197.00 6.340Max Rating of Perceived
Exhaustion(RPE)
13 20 17.00 1.91
% Body Fat 7.3 29.7 17.88 7.20
Maximal Values forAcute Exercise
Test
Minimum Maximum Mean
Statistics Statistics Statistic Std. deviation
VO2 Max Absolute (mL/min)
1825.2 3056.8 2318.61 339.45
VO2 Max Relative(mL/Kg/min)
26..87 41.40 35.27 5.00
Respiratory Exchange Ratio (RER)
0.84 0.98 .093 0.044
Heart Rate(bpm) 154.33 176.5 167.20 6.14
Table 1: This table shows the mean for all the values measured
for the total number of subjects during the initial visit of the
maximal exercise test.
Table 2: This table shows the value measured during the last
stage of acute bout of exercise test. On the right shows the mean
of the Statistic and standard deviation.
Figure 1: This figure shows pre and post of STAT3 gene
expression on acute bout of exercise. There was no significant
(p=0.483) increase in STAT3 gene following acute bout of
exercise.
Figure 2: This figure shows pre and post of CXCR4 gene
expression on acute bout of exercise. There was no significant
(p=0.867) increase in CXCR4 gene following acute bout of
exercise.
0.75
0.8
0.85
0.9
0.95
1
1.05
pre post
Signal Transducer and Activator of Transcription 3
0.99
1
1.01
1.02
1.03
1.04
1.05
1.06
1.07
pre post
Chemokine (C-X-C) motif Receptor 4
STAT
3 m
RN
A/1
8sC
XC
R4
mR
NA
/18s
Slide Number 1
