Earth Science Institute of Orleans, France UMR 6531 of CNRS - University of Orléans

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Earth Science Institute of Orleans, France UMR 6531 of CNRS - University of OrléansGroup « Organic Matter in water, sols, sediments and rocks »

Personal involved in RECIPE :

Fatima LaggounLaure Comont (PhD student)Jean-Robert Disnar

Patrick Albéric (DOC, water chemistry)Christian Défarge (µmorphology - cryo-SEM)

Nathalie Lottier (GC-MS / Capillary eletrophoresis)Marielle Hatton (C, N, S & palynofacies preparation)

Charquemont, Recipe meeting 23-27th Oct 2003

Appointment of staff and students to RECIPE& relevant problems :

Application for many calls: (i) PhD grants from public Dpts, (ii) Marie Curie post-doc grant (6th Framework Prog.)

Oct. 2001 to Nov. 2002: a PhD student A. Fleury (2 Jura sites)

Oct. 1st 2003: Laure Comont [3 years PhD grant from Research Dpt of Local Adminitr. District (Region Centre)]

Eval. MC grant: C. Applicant: Martin Kainz, Univ Victoria, Canada. Project: Biomolecular source identification of OM in peatland ecosystems (lipid biomarkers, i.e. fatty acid methyl esters)

Philipp Steinmann: invited staff at ISTO in the framework of RECIPE (1 month (April or May 2004) fund from Univ Orleans). Project: (i) porewater sampling by peepers & isotopic analyses of CO2, CH4 and acetates; (ii) Archaea biomarkers .



Analysis progress:

Sampling on Oct. 20012 sites in the Jura: Le Russey (France): 5 cores (70 Samples)La Chaux d’Abel (Switzerland): 6 cores (91 Samples)

Analyses:CNS analyses, identification & counting of organic fragments, counting of bacteria, sugar analyses of peat samples and vegetation (8 sphagnum sp., 1 polytrichum, 2 Erioph. sp.).

Participation to congresses:9th Congress of Sedimentology (Oct., 14-16th 2003, Bordeaux)7th Meeting of Soil Study (Oct., 22-24th 2002, Orleans)

ENVIRONMENT PARCELS SAMPLING VEGETATION

FENCA-1 8

CA-2 11

CA-3 6

CA-4 8

CA-5 9

INTACT BOG CA-6 8

CA 3

Numbers of samples

Vegetation, litters (Polytrichum and Sphagnum, erected stems), black litter (Polytrichum), 0-4, 6-8, 15-20, 22-24,

25-30, 33-38, 40-45, 50-55, 57-62, 65-70

Polytrichum and Shagnum fallax

vegetation, less decomposed litter, more decomposed litter, 0-4, 6-10, 10-15, 20-25, 28-31, 37-42, 50-55, 56-58

(a), 58-60 (b), 65-70, 70-75, 75-80

Eriophorum vaginatum and

S.fallax

TRANSITIONAL ZONE

Vegetation, litter, 2-4, 8,5-10,5, 8,5-14, 14-19, 19-25, 25-30, 32-37,

38-43

Transition Polytrichum and less

Shagnum

BOG near intact zone CA-6,

semi-wooded environment

vegetation , 0-3, 3-6 (young litter), 6-10 (sink litter), 10-15 (blonde litter), 15-20, 20-25, 25-30, 30-35, 35-40,

40-43, 43-47

Eriophorum vaginatum and

S.fallax

Vegetation, top, 0-4, 6-10, 4-10, 10-14, 14-20, 20-25, 25-30, 32-37, 40-45, 45-50, 50-55, 55-60, 60-65

Polytrichum and S.fallax

4-10, 10-18, 18-20, 20-23, 26-31, 33-36, 41-45, 48-53, 53-58, 58-62, 63-68, 69-74

High pine forest, near exploited peat (possible drainage

effect)

Sphagnum (desiccated vegetation), 0-5, 7-11, 13-16 cm

Sampling in la Chaux d’Abel

Analyses made in ORLEANS since November 2001Samples/cores Leco/CNS Micromorphol.Anal. Sugars Bacteria counting

Sphagnum X XEriophorum X XPolytrichum X XCore CA XCore CA.1 X X XCore CA.2 X X XCore CA.3 X X XCore CA.4 X X XCore CA.5 X X X XCore CA.6 X X X X

ENVIRONMENT PARCELS SAMPLING VEGETATION

Ry.A 9 (ISTO)

Ry.B 11 (ISTO)

Ry.C S. fallax

Ry.C' Same as Ry C, taken by D.Gilbert S. fallax

Ry.D S. fallax

4 (Estelle)

10 (ISTO)

Ry.E Same as Ry.D, replicates taken by D.Gilbert S. fallax

Ry.F Same as Ry.D, replicates taken by D.Gilbert S. fallax

INTACT 1 ??

5 (Estelle)

9 (ISTO)

INTACT 2 Same

INTACT 3 Replicates taken by D.Gilbert Same

Intact pine forest 18 (ISTO)

Cores taken in November 2001

Complement cores taken in 5 juin

2002Numbers of

samples

Bare peat, tendancy of FEN,

dominance of Eriophorum

angustifolium, less E. vaginatum

Sphagnum (vegetation), litter, 5-10, 15-20, 25-30, 34-38, 42-47, 52-57, 70-75 cm

S.rubellum, S.magellanicum,

P.alpestreVegetation, less decomposed litter, more decomposed litter, 0-3,

3-5, 5-10, 10-15, 15-20, 20-25, 25-30, 30-34, 34-38, 38-42, 42-47, 47-52, 52-57, 60-65 cm

Dominance of S.fallax, more

advanced stage than A

Replicates taken only in the top of the core, same as A et B

More advanced regeneration ?

Lower area, deep peat?

18-23, 28-33, 38-43, 48-53 cm (by Estelle) ; vegetation (+litter), less decomposed litter, more decomposed litter, 0-2, 11-15, 18-23, 25-27, 28-33, 30-33, 38-43, 48-53, 53-58,

58-63, 63-68, 68-73 cm

Higher area with more diversified vegetation

Intact zone ???

15-20, 25-30, 35-40, 45-50, 55-60 cm (par Estelle) ; vegetation, less decomposed litter, 0-4, 8-10, 10-16 (more decomposed

litter), 16-20, 27-32, 36-41, 46-51, 51-56, 56-61, 61-66, 66-71 cm

S.fallax, S.rubellum, Polytrichum, caluna

vulgaris

block and samples: sphagnum, litter, under-litter, litter in catotelme, surface peat

NEW INTACT

eastern area

Vegetation, 0-2, 2-4, 4-8, 10-14, 14-18, 18-22, 25-28, 31-34, 37-40, 43-46, 49-52, 55-58, 61-64, 67-70,

73-76, 79-82, 85-88

Analyses made in ORLEANS since November 2001CORES Leco/CNS Micromorphol. Anal. Sugars Bacteria counting

Ry.A X XRy.B X X XRy.D X X X

Ry.I ?? X X XRy.New Intact X

C/N ratios and microscopic counting Chaux d’Abel/fen (minetrophic zone)-Newly

restauredCA.1 Sphagnum & Polytrichum CA.2 Sphagnum & Eriophorum

C/N ratios and microscopic counting

CA.5 Sphagnum & Polytrichum CA.4 Sphagnum & Eriophorum

Chaux d’Abel/bog (ombrotrophic zone) Newly restaured


C/N ratios and microscopic counting Chaux d’Abel Intact zone (CA 6)


Regeneration ?

C/N ratios and microscopic counting (Le Russey) Ry.B – no regeneration

?Ry.D – more advanced

stage

C/N ratios and microscopic counting (Le Russey)Ry.I – Intact peat

Regeneration


S. rubellum

Rhm

Gal

%

Biomarkers of organic sources

(sugar analyses)

ERIOPHORUMERIOPHORUM

XyloseXyloseArabinoseArabinose

POLYTRICHUMPOLYTRICHUMMannose HMannose H

SPHAIGNESSPHAIGNES

RhamnoseRhamnoseGalactoseGalactose

ERIOPHORUM

Ara

Xyl

%

POLYTRICHUM Man H

%

S. fallax

Rhm

Gal

%

vegetation


sugar analyses


POLYTRICHUMPOLYTRICHUM

SPHAIGNESSPHAIGNES


Mannose HMannose HRhamnoseRhamnoseGalactoseGalactose

La Chaux d’Abel / bog

- exploited zone

1

2

3

% Arabinose et Xylose

2

Pro

fondeur

(cm

)

% Mannose

3

Pro

fondeur

(cm

)

% Rhamnose et Galactose

1Pro

fondeur

(cm

)



POLYTRICHUMPOLYTRICHUM

SPHAIGNESSPHAIGNES


Mannose HMannose HRhamnoseRhamnoseGalactoseGalactose

La Chaux d’Abel / INTACT ZONE

% Glucose

Pro

fondeur

(cm

)

1

% Xylose

Pro

fondeur

(cm

)

1

% Man, Ara, Fuc et Rib

Pro

fondeur

(cm

)

2

1

2

sugar analyses


bacteria counting

Observations x1000 en épifluorescence

50 m

5 m 3 m


bacteria counting (La chaux d’Abel)

Taille échantillon Taille échantillon de départ de départ

Concentration enConcentration en bactéries/g de bactéries/g de tourbe fraîchetourbe fraîche

BIOMASSEBIOMASSECarboneCarbone

BiovolumeBiovolume

INTACT Zone

Pro

f ondeu

r (c

m)

Pro

f ondeu

r (c

m)

Abondance b/g.ech.sec. Biomasse mgC.g

Pro

fondeu

r (c

m)

Pro

f ondeu

r (c

m)

EXPLOITED Zone

Abondance b/g.ech.sec. Biomasse mgC.g

CA 5 CA 6Charquemont, Recipe meeting 23-27th Oct 2003

Bacteria counting (Le Russey)Ry.B – Exploited zone


Ry.D – Exploited zone

Bacteria counting (Le Russey)

Transition (C/N, %OF)

Regeneration


Ry.I – Intact zone

Bacteria counting (Le Russey)

Regeneration


Replicates ? (Ry A & B: less advanced regeneration stage)


Documents

Earth Science Institute of Orleans, France UMR 6531 of CNRS - University of Orléans