Linear Sequence: unnamed sequence

Cleavage code Enzyme name code

blunt end cut

5’ extension

3’ extension

cuts 1 strand

Available from NEBHas other supplierNot commercially available∗ : cleavage affected by CpG methylation#: cleavage affected by other methylation(enz. name): ambiguous site

Display: - NEB single cutter restriction enzymes

- Main non-overlapping, min. 100 aa ORFs

GC=39%, AT=61%

209 aa∗ BsiEI ∗ Hpy166II

215 aaHaeIII HpyCH4III

272 aaHpyCH4III PsiI

1 3745

DraIBsrGISphI

BstXI

BtsIBlpI

EciIFokI∗ BspEIBtsCIBsu36I

∗ PaeR7I∗ XhoI

∗ Hpy99IAvrII∗ BcgI

HpaIDraIII

KpnIAcc65I

BanI∗ BsmBI

BanIISacI

Eco53kI

∗ FauI∗ BstBIBbvCI∗ BceAI

BbsIMscIEaeI

PvuII#PflMI

BspMIBfuAISbfI

BsaI∗ AleITth111IPflFI

AflII∗ BtgZI

NcoIBtgI

∗ #ClaI∗ #BspDI

BglIIBstEII