DYN R&D a member of the DYN Diagnostics Group

DYN Diagnostics

DYN R&D DYN LabsDYN Medical

Equipment

What How and Where

What? Genotyping DNA markers:

• Trait specific markers.

• Purity testing markers

How? Ready to use kits or full services.

Where? Dominant in local market. Significant

presence in various countries.

Background

• DYN R&D was founded in 2007 to provide advanced molecular assays for the seeds industry, including:

DNA marker tests for breeders and vegetables seed producers

Efficient diagnostic tools: fast, accurate and cost effectives.

The world QA standard pre- and post-control purity tests of parent lines and hybrid seeds

About Us

• Our R&D partner is the BonTom tomato breeding group, at the R H Smith Faculty of Agriculture, Food and Environment, the Hebrew University of Jerusalem, headed by world expert: Prof. Haim D. Rabinowitch.

• Our work professional teams consist of: Molecular biology experts; Bio-technology engineers; Application support group; A leading team of plant geneticists, plant physiologists and plant pathologists.

• Being a member of DYN Diagnostics Group, guaranteed our financial strength and enables significant investments in R&D.

About Us

Genotype DNA markersAdvance Vs. Conventional

420 min for 96 results

Up to 600 results per day

~75 min for 96\384 results

Thousands results per day

DNA extraction

PCR (~150 min)

Enzymatic restriction (180 min)

Agarose gel separation (60 min)

Analyze 96 results (~30 min)

Short PCR (>60 min)

Melt (~15 min)

Automatic analyze 96 results (~2 min)

Genotype DNA marker testsWorkflow

Plant tissue

X96SuperFast DNA

extraction, 2min

Amplification +

Melt, 75minAnalysis,

20min

• SimpleProbe probes consist of only one oligonucleotide, which carries a fluorescent dye and a quencher linked directly to each

other.

•. With SimpleProbe format,

LightCycler® 480 Software

monitors the decrease in

fluorescence during the post

PCR melting step.

• Because of their conformation, fluorescence is quenched in solution but activated after binding to the target sequence.

Tests menu

• Tomato markers:• MAS, MAB markers, pathogen resistance and

morphological traits.

• Tomato Purity Markers: Unique set of fingerprinting markers

• Pepper, melons and more.

Tomato Ty-1 test

Why Tomatoes

• Tomato seeds are sold for an average of 10,000$ a kilo (of seeds).

• The Tomato seeds market is a very competitive as new varieties and advanced varieties are released annually.

• Seeds companies invest heavily to meet the growing demand for improved tomato hybrid seeds.

Why Tomato purity?

• Tomato hybrid seeds production consists of hand pollination of the stigma.

• Purity verification is highly important as impurities cause heavy losses to both the seed company and the growers.

Universal code for tomato\pepperhybrids

• Product Objectives:• Positive verification of the product authenticity• Detecting even minute self pollination impurities. • Detection of any other impurities, e.g., mechanical mix, error

pollination, wrong labeling, and more.

• Product Principals:– Universal test- all genotypes are tested using method single

methodology.– Maximum automation and objective process free of human

input. Hardware & Software.– Multi parameter test and yet time and cost effective.

Test limitations:

• Test ∞ parameters,– For example: sequence whole genome

– Danger: Detect even Pure seeds as Impure.

False positive.

• Test 1 parameter,– For example: test one DNA marker-Tm2a

– Danger: Detect different- Impure seeds as identical- Pure. False negative.

•Test ∞ SNP’sparameters

Product principals, terminology.

• Each sample is being tested for 8 SNP’s: Test1- Test8. Test1, test2,… Test8. T1, T2,…T8.

• Each Test (T1-T8) results in one of 3 possible (output)

alternatives: 1 or 2 or 3.

RedHomozygote aaa

BlueHomozygote AAA3

GreenHeterozygoteAa2

Product principals, terminology.

• Each sample tested for 8 SNP’s: Test1- Test8.

Test1, test2,… Test8. T1, T2,…T8.

• Each Test (T1-T8) has 3 possible results (output): 1 or 2 or 3.

T8T7T6T5T4T3T2T1

1223

38= 6,561 different codes. For hybrids.

28= 256 different codes. For parents.

A new standard QA test for tomato hybrids.

The worlds 1st hybrid barcode printer

Some files

Why

World breeding expert partners.

Very efficient tomato purity test

Cost effective.

Flexible to customer needs.

Ready to use kits or full service.