Embed Size (px)
Citation preview
Thermo ScientificDRI Oxycodone AssayDrugs of Abuse Testing
Oxycodone, a semi-synthetic opioid, is prescribed for pain management for patients with moderate to severe pain. The analgesic properties of oxycodone
are similar to codeine and morphine, but more potent than morphine. Oxycodone has a higher dependency potential. Drug abusers crush the pills into powder and
snort for a faster effect which may result in a potentially fatal outcome.The DRI® Oxycodone assay is a liquid, ready-to-use assay with specific
antibodies that detect oxycodone and oxymorphone without significant cross reactivity to other opiate compounds.
Part of Thermo Fisher Scientific
© 2008 Thermo Fisher Scientific Inc. All rights reserved. All the trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
2008 08 01
Thermo ScientificDRI Oxycodone AssayDrugs of Abuse Testing
DiagnosticsU.S.A.46360 Fremont Blvd. Fremont, CA 94538 USATel. 800-232-3342Fax: 510-979-5002CanadaTel. [email protected]. + 49 851 88689 [email protected] Tel. + 61 2 9649 [email protected] Tel. + 34 93589 [email protected] KingdomTel. + 44 1727 [email protected]
• Outstanding sensitivity and specificity
• Exhibits 100% cross-reactivity to oxymorphone with very low cross reactivity to other opiate compounds
• Excellent correlation with GC/MS• Optimized for 2 cutoff levels,
300 ng/mL and 100 ng/mL• Fully automated results in
minutes• Increased value to drug test
panel for clients• Liquid, ready-to-use reagents in
convenient packaging for use on a variety of chemisty analyzers
Specific Immunoassay for Detecting Oxycodone Qualitative
100 ng/mL Cutoff 75 ng/mL 100 ng/mL 125 ng/mL
Mean (mA/min) = 348 370 389
SD = 2.1 1.9 2.0
% CV = 0.6 0.5 0.5
300 ng/mL Cutoff 225 ng/mL 300 ng/mL 375 ng/mL
Mean (mA/min) = 429 458 479
SD = 2.2 2.4 2.4
% CV = 0.5 0.5 0.5
Semi-Quantitative
75 ng/mL 100 ng/mL 125 ng/mL
Mean (ng/mL) = 73 98 123
SD = 2.4 2.9 3.0
% CV = 3.3 2.9 2.4
225 ng/mL 300 ng/mL 375 ng/mL
Mean (ng/mL) = 227 303 375
SD = 5.0 9.0 10.2
% CV = 2.2 3.0 2.7
Within Run Precision
Qualitative
100 ng/mL Cutoff 75 ng/mL 100 ng/mL 125 ng/mL
Mean (mA/min) = 348 370 389
SD = 2.9 3.2 3.1
% CV = 0.8 0.9 0.8
300 ng/mL Cutoff 225 ng/mL 300 ng/mL 375 ng/mL
Mean (mA/min) = 429 458 479
SD = 3.7 4.1 3.8
% CV = 0.9 0.9 0.8
Total Precision
Semi-Quantitative
75 ng/mL 100 ng/mL 125 ng/mL
Mean (ng/mL) = 73 98 123
SD = 2.9 3.6 4.9
% CV = 4.0 3.7 4.0
225 ng/mL 300 ng/mL 375 ng/mL
Mean (ng/mL) = 227 303 375
SD = 8.2 11.5 14.7
% CV = 3.6 3.8 3.9
Compound Concentrations (µg/mL)
6-Acetylmorphine 50
Codeine 500
Dihydrocodeine 100
Heroin 300
Hydrocodone 75
Hydromorphone 30
Levorphanol 200
Morphine 350
Morphine-3-glucuronide 900
Norcodeine 1000
Normorphine 1000
SensitivityThe sensitivity of the assay using the negative calibrator is 4.9 ng/mL:
Cross ReactivityThe following structurally related compounds tested negative with the DRI Oxycodone assay using a cutoff of 100 ng/mL:
Catalog Number Product Description Size (mL)
100248 Oxycodone Reagent Kit 70
100249 Oxycodone Reagent Kit 500
100250 Oxycodone 100 Calibrator 10
100251 Oxycodone 300 Calibrator 10
100252 Oxycodone 500 Calibrator 10
100253 Oxycodone 1000 Calibrator 10
100254 Oxycodone 100 Control 2 x 10
100255 Oxycodone 300 Control 2 x 10
Order 1-800-232-3342
Interferent Level (mg/dL) Interferent Level (mg/dL)
Acetone 1000 Hemoglobin 300
Ascorbic acid 1500 Human serum albumin 500
Creatinine 500 Oxalic acid 100
Ethanol 1000 Riboflavin 7.5
Galactose 10 Sodium Chloride 1000
Glucose 3000 Urea 2000
InterferenceNo significant interference was observed from the following substances:
Method Comparison
Qualitative vs. GC/MSIn sample correlation studies using 144 clinical samples, the overall concordance at both cutoff levels, 100ng/mL and 300ng/mL, is 97.2%.Semi-Quantitative vs. GC/MSDRI Oxycodone correctly detected 59 samples as positive and 84 as negative using 100 ng/mL as cutoff calibrator.
GC/MS vs DRI OxycodoneDemings y = 1.00 - 1, r = 0.982
Least Square Regression:Slope: 0.98Intercept: 2.00r: 0.982N: 139
GC/MS (ng/mL)
DRI (
ng/m
L)
1000
800
600
400
200
00 200 400 600 800 1000
