Dr.AmaniYousefOwaidah AssistantProfessor

PersonalData

Nationality|Saudi

Department|ClinicalLaboratorySciences

OfficialUoDEmail|ayowaidah@uod.edu.sa

OfficePhoneNo.3331347

LanguageProficiency

Language Read Write SpeakArabic Advanced Proficient AdvancedEnglish Advanced Advanced AdvancedAcademicQualifications(Beginningwiththemostrecent)

Date AcademicDegree PlaceofIssue AddressMay13,2014 PhD UniversityofBristol Bristol,UnitedKingdomOct13,2009 MScwithMeritin

TransfusionandTransplantationSciences

UniversityofBristol Bristol,UnitedKingdom

Sep8,2004 BScMedicalLaboratorySciences

KingFaisalUniversity Dammam,SaudiArabia

PhD,MasterorFellowshipResearchTitle:(AcademicHonorsorDistinctions)

PhD CellularandMolecularMedicine(StemCellBiologyandCartilagetissueengineering)Master MScwithMeritinTransfusionandTransplantationSciencesProfessionalRecord:(Beginningwiththemostrecent)

JobRank PlaceandAddressofWork DateAssistantProfessor Universityof

DammamDammam,SaudiArabia

September2014-present

ResearchScientist KingFahdSpecialistHospital

Dammam,SaudiArabia

August2015-July2016

SeniorTechnologist(BloodBank)

KingFahdMilitaryMedicalComplex

Dhahran,SaudiArabia

April2007-April2008

JuniorTechnologist(BloodBank)

KingFahdMilitaryMedicalComplex

Dhahran,SaudiArabia

December2004-April2007

ScientificAchievements

PublishedRefereedScientificResearches(InChronologicalOrderBeginningwiththeMostRecent)# NameofInvestigator(s) ResearchTitle PublisherandDateofPublication1 AmaniOwaidahandWael

Kafienah• Cartilage Tissue

Engineering Using Embryonic Stem Cells, Reference Module in Biomedical Sciences.

Elsevier/August24,2016doi:10.1016/B978-0-12-801238-3.99808-8

ContributiontoScientificConferencesandSymposia# ConferenceTitle PlaceandDateoftheConference ExtentofContribution1 SaudiSocietyof

TransfusionmedicineJeddah,SaudiArabiaApril2016

Speaker

2 1stSaudiImmunohematologygroupmeeting

Dammam,SaudiArabiaDecember2015

SpeakerandModerator

3 AdvancesinMolecularBiology

Dammam,SaudiArabiaOctober2014

Speaker

4 StemCellSymposium Cardiff,UnitedKingdomJanuary2014

Speaker

5 InternationalCartilageRepairSociety(ICRS)11thcongress

Izmir,TurkeySeptember2013

Speaker

6 2ndupdateinLaboratoryMedicineMeeting

Dammam,SaudiArabiaApril2013

Speaker

7 2ndSouthWestRegionalRegenerativeMedicineMeeting

Bristol,UnitedKingdomSeptember2012

Speaker

8 InternationalCartilageRepairSociety(ICRS)10thcongress

Montreal,CanadaMay,2012

Speaker

ScientificAwards

• Certificate of excellence by the Saudi Society of Genome and Molecular Oncology (SSGMO) December2013.

• Distinguished Saudi scholars in a ceremony held at the Royal Embassy of Saudi Arabia by HRH PriceMohammedbinNawafAl-Saud(SaudiAmbassadortotheUnitedKingdom)inSeptember2012.

MembershipofScientificandProfessionalSocietiesandOrganizations

• ChairpersonofRedcellImmunohematologygroupoftheSaudiImmunohematologyDiscussiongroup

TeachingActivities

UndergraduateCourseCoordination# CourseTitleandCode Coordination Undergrad. From to1 ClinicalBloodBanking Coursecoordinator 4thyear Sep18-2016 Jan-26,2017StudentAcademicSupervisionandMentoring# Level NumberofStudents From to1 4thyear 1student Sep18,2016 June15,2017PersonalKeyCompetenciesandSkills:(Computer,Informationtechnology,technical,etc.)

• Cell culture and cryo-storage - Generation of human induced pluripotent stem cells (hiPSC) and bonemarrowmesenchymalstemcells.Dailymaintenancethroughtheobservationofcellhealth(visualizationofcells with light microscope and culture media assessment) and then culture of cell lines (feeding,division/expansionandcryostorage).

• Characterizationofpluripotentstemcellandmesenchymalstemcells:-AmajorstageinmyPhDstudies

the characterization of the cell lines through quantitative PCR, immunofluorescence staining,immunohistochemistryandflowcytometry.

• Stem cell differentiation studies – After the successful maintenance of induced pluripotent stem cell

cultures,thecoreofmyPhDwastodesigndifferentiationprotocoltoinducechondrogenicdifferentiationin the human induced pluripotent stem cells. This required extensive understanding of embryonicdevelopmentandcellsignallingpathways.

• 3DTissueengineering–Thiswasimportanttoinvestigatetheabilityofthedifferentiatedhumaninduced

pluripotent stem cells chondroprogenitors to generate a 3D cartilage construct suitable for cell-basedtherapies. The Stem Cell Biology group in the University of Bristol, using mesenchymal stem cells,polyglycolicacidscaffoldandrecombinantgrowthfactors,developedthetissueengineeringprotocol.ForthehiPSC,myPhDwastoinvestigatethefactorsthatinduceitschondrogenicdifferentiation.

• ELISA and colorimetric assay use and result processing - This was important in the measurement of

cartilageextracellularmatrixcomponentssuchastypeIandtypeIIcollagensandsulphatedproteoglycans.

• Histology and immunohistochemistry – These techniqueswere used to analyze the cellularmorphologyand the quality of the 3D tissue engineered cartilage using a wide range of stains. During my PhD Iexperiencedinthedevelopmentandoptimizationofimmunohistochemistrystains.

• Dataanalysis–ThroughoutmyPhDdataanalysishasbeen important.DuringmyrecentPhDstudies the

statisticalpackagePRISM(v5.0c)wasusedtoinvestigatewithin/betweengrouptrends.

• Cell signaling experimentation –Daily experimentation at theUniversity of Bristol was the treatment ofhiPSCwithvarious factors toactivatesignalingpathways involved in theself-renewalanddifferentiation.ThisincludedtheTGFβ,BMP,FGF,andActivinsignalingpathways.

• Blood transfusion and blood banking skills - During my work at King FahdMilitaryMedical Complex in

Dhahran, I experienced all routine and specialized blood banking tests that included ABO +RH forwardgrouping, reversegrouping,Antibodyscreeningand identification,directand indirectantihumanglobulintest, Compatibility testing, resolving ABO discrepancies, acid elution, pre-warm technique, salinereplacementat4oC,coldagglutinins,TypespecificindirectantihumanglobulintestTSIAT,Neutralizationbypooledplasmaandantigentyping).

• Donorregistry,selectionandphlebotomy–Iinstalledacomputerizedprogrammeforthedonorregistryat

KFMMC.Along-sideMrJosephRobinson(Bloodbanksupervisor)tookdecisionroleindonorselection.

• Bloodcomponentpreparationandstorage-duringmytimeatKFMMC,bloodcomponentpreparationandstoragewasanimportanttaskinmyjobdescription.

• Generationandmaintenanceoflabrecordsandstatistics.Adheretostatutoryhealthandsafety

requirements.

LastUpdate

5/12/2016