Marine animal models in evolution & development 1 – 12 July 2013 | Fiskebäckskil, Sweden

Preliminary Agenda PIs lectures: each PI has 1 hour slot that should include 45 minutes lecture + 15 minutes discussion. Please stick to this schedule. Sweatbox: after the lecture, all other PIs leave the room while students ask questions to the speaker for about half an hour. SUNDAY JUNE 30, 2013 - ARRIVAL DAY

17:30 - 19:00 Welcome buffet

19:00 - 20:00 Welcome and introduction to the course - Detlev Arendt

20:00 – 21:00 Keynote lecture by Claus Nielsen: Marine life at Gulmarsfjord

MONDAY JULY 1, 2013 07.30 – 08.30 BREAKFAST

Participants split in 4 working groups (WG)

08.30 – 12.30 WG 1 and 2 Boat trip: plankton, benthic meio- and macrofauna collection

WG 3 and 4 Safety briefing Plankton collection by teachers

12.30 – 13.30 LUNCH

13.30 – 17.30 WG 3 and 4 plankton inspection WG 1 and 2 safety briefing

Inspection of plankton and benthic meio- and macrofauna Collection of plankton with rowing boat

17.30 – 18.30 DINNER

18.30 – 19.30 Wrap up by Claus Nielsen

19.30 – 21.00 Poster Session

TUESDAY JULY 2, 2013 07.30 – 08.30 BREAKFAST

08.30 – 12.30 WG 3 and 4 Boat trip: plankton, benthic meio- and macrofauna collection

WG 1 and 2 Plankton collection with rowing boats

12.30 – 13.30 LUNCH

13.30 – 14.00 Introduction to comparative antibody staining (Ina Arnone)

14.00 – 15.00 Introduction to 4D microscopy/SIMI BioCell rotifer, acoel, tardigrade, gastrotrich (Andreas Hejnol & Sabrina Schiemann)

15.00 – 15.30 Introduction to microinjections, cell lineaging and 4D microscopy on Parhyale (Frederike Alwes)

15.30 – 17.30 Inspection of plankton and benthic meio- and macrofauna

Collection of plankton with rowing boat

Fixation of samples Start of antibody staining

17.30 – 18.30 DINNER

18.30 – 19.00 Introduction to CLEM (Harald Hausen and Gaspar Jékély)

19.00 – 20.00 Lecture: Phylogeny reconstruction and phylogeny interpretation (Max Telford)

20.00 – 20.30 “Sweatbox” (Max Telford)

20.30 – 21.00 Introduction to Light Sheet Fluorescence Microscopy and Open SPIM (Olaf Selchow and Pavel Tomancak)

WEDNESDAY JULY 3, 2013 08.00 – 09.00 BREAKFAST

09.00 – 09.30 Introduction to 2 color fluorescent in situ hybridization (Ina Arnone/Evelyn Houliston)

09.30 – 10.00 Introduction to comparative in situ staining (Heather Marlow)

10.00 – 10.30 Introduction to the flatworm Maritigrella (Bernhard Egger)

10.00 – 12.00 Antibody staining LSFM 4D microscopy Microinjections / Cell lineaging Collected samples

fixation and preparation

12.00 – 13.00 LUNCH

13.00 – 17.30 Antibody staining LSFM 4D microscopy Microinjections / Cell lineaging Collected samples

fixation and preparation

17.30 – 18.30 DINNER

19.00 – 20.00 Lecture (Ina Arnone)

20.00 – 20.30 Sweatbox (Ina Arnone)

THURSDAY JULY 4, 2013 08.00 – 09.00 BREAKFAST

09.00 – 09.30 Introduction to Nematostella vectensis (Heather Marlow)

09.30 – 10.00 Introduction to Clytia hemisphaerica (Evelyn Houliston)

10.00 –12.00 Antibody staining In situ hybridization (WMISH)

Microinjections / Cell lineaging

4D microscopy LSFM

12.00 – 13.00 LUNCH

13.00 – 17.30 Antibody staining In situ hybridization (WMISH)

Microinjections / Cell lineaging

4D microscopy LSFM

17.30 – 18.30 DINNER

18.30 – 19.30 Lecture (Detlev Arendt)

19.30 – 20.00 Sweatbox (Detlev Arendt)

20.00 – 21.00 Lecture (Gaspar Jékely)

21.00 – 21.30 Sweatbox (Gaspar Jékely)

FRIDAY JULY 5, 2013 08.00 – 09.00 BREAKFAST

09.00 – 09.30 Introduction to sea urchins (Ina Arnone)

09.30 – 10.00 Introduction to Platynereis dumerilii (Detlev Arendt)

10.00 – 12.00 In situ hybridization (WMISH) Microinjections / Cell lineaging 4D microscopy

LSFM

12.00 – 13.00 LUNCH

13.00 – 14.00 Lecture: Confocal laser scanning and reflection microscopy techniques demo (Gaspar Jékely)

13.30 – 17.30 In situ hybridization (WMISH) Microinjections / Cell lineaging 4D microscopy

LSFM

17.30 – 18.30 Lecture on fossils (Graham Budd)

18.30 – 19.00 Sweatbox (Graham Budd)

19.00 – 19.30 Introduction to field trip and fossils (Graham Budd)

20:00 - BBQ Bag packing for trip

The field-trip is organized and guided by Graham Budd, professor of Paleontology at Uppsala University.

More information on the field trip will be distribute soon.

SATURDAY JULY 6: Paleontological Field Trip SUNDAY JULY 7: Paleontological Field Trip

MONDAY JULY 8, 2013 08.00 – 09.00 BREAKFAST

09.00 – 09.30 Introduction to transgenic tools in emerging models (Michalis Averof)

09.30 – 10.00 Introduction to image registration (Gaspar Jékely)

09.30 – 12.00 Microinjections / cell lineaging

Finish antibody staining

Finish in situ hybridization (WMISH)

4D microscopy / LSFM

Image registration

12.00 – 13.00 LUNCH

13.30 -- 17.30 Microinjections

17.30 – 18.30 DINNER

18.30 – 19.00 Introduction to Ascidians and electroporation (Patrick Lemaire)

19.00 – 20.00 Lecture (Evelyn Houliston)

20.00 – 20.30 Sweatbox (Evelyn Houliston)

20.30 -- Night sampling (full moon)

TUESDAY JULY 9, 2013 08.00 – 09.00 BREAKFAST

09.00 – 09.30 Introduction to microinjections in several species and lineaging by tracer dye injections (Mark Q. Martindale)

09.30 – 10.00 Introduction to cell lineaging in Ophiuroids (Paola Olivieri)

10.30 – 12.00 Microinjections/cell lineaging

Transgenic tools in Parhyale Electroporation Confocal / LSFM Image registration

12.00 – 13.00 LUNCH

13.00 – 17.30 Microinjections/cell lineaging

Transgenic tools in Parhyale Electroporation Confocal / LSFM Image registration

17.30 – 18.30 DINNER

19.00 – 20.00 Lecture (Pavel Tomancak)

20.00 – 20.30 Sweatbox (Pavel Tomancak)

20.30 – 21.30 Lecture (Michalis Averof)

21.30 – 22.00 Sweatbox (Michalis Averof)

WEDNESDAY JULY 10, 2013

08.00 – 09.00 BREAKFAST

09.00 – 10.00 Lecture (Harald Hausen)

10.00 – 10.30 Sweatbox (Harald Hausen)

10.30 – 12.00 Microinjections/cell lineaging

Transgenic tools in Parhyale Electroporation Confocal / LSFM Image registration

12.00 – 13.00 LUNCH

13.00 – 17.30 Microinjections/cell lineaging

Transgenic tools in Parhyale Electroporation Confocal / LSFM Image

registration

17.30 – 18.30 DINNER

18.30 – 19.30 Lecture (Mark Q. Martindale)

19.30 – 20.00 Sweatbox (Mark Q. Martindale)

20.00 – 21.00 Lecture (Patrick Lemaire)

21.00 – 21.30 Sweatbox (Patrick Lemaire)

THURSDAY JULY 11, 2013 08.00 – 09.00 BREAKFAST

09.00 – 12.00 Microinjections/cell lineaging

Transgenic tools in Parhyale Electroporation Confocal / LSFM Image

registration

12.00 – 13.00 LUNCH

13.00 – 17.30 Finalizing all ongoing experiments

17.30 – 18.30 DINNER

18.30 – 19.30 Lecture (Andreas Hejnol)

19.30 – 20.00 Sweatbox (Andreas Hejnol)

FRIDAY JULY 12, 2013 08.00 – 09.00 BREAKFAST

09.00 – 12.00 Studentsʼ presentations and discussions

12.00 – 13.00 LUNCH

13.00 – 17.30 Students presentations and discussion

17.30 – 18.30 DINNER

18.30 – 19.30 Course wrap-up Feedback session

20.00 onwards PARTY!

SATURDAY JULY 13, 2013 08.30 – 09.30 BREAKFAST

Departures

Sponsored by

Roche Invitrogen Dianover