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DOI 10.13350 j.cjpb.180122 ·/· 9EéµHIêë/ìÿijhæ 7êä ú]%&(ï1&Þ*=6\ú] 563000 !"ûüâY犮狅犫犪犮狋犲狉犻狌犿狊犿犲 犿犪狋犻狊 MS åJ"V.³yÀè3âYÓ3fg EFyÁowÚ MSVWm]¤l3Qÿ¯tï1|phE$¨d,^ëfg9Ë MSyH[ -¹·º£y*+}oVJ/*+,fgûüâY9Ë/ -./01R378.91 23456278116735234 2018 01009606 犑狅狌狉狀犪犾狅 犘犪狋犺狅 犲狀犅犻狅犾狅 犵狔.2018Jan 13 ): 96-101. 犃犱狏犪狀犮犲狊犻狀狉犲狊犲犪狉犮犺狅狀犵犲狀犲狋犻犮犲狀犵犻狀犲犲狉犻狀犵狅犳狉犲犮狅犿犫犻狀犪狀狋犮狅犫犪犮狋犲狉犻狌犿狊犿犲 犿犪狋犻狊 LIXiang ZHOUBiying 犇犲 犪狉狋犿犲狀狋狅 犘犪狉犪狊犻狋狅犾狅 犵狔 犣狌狀 犻犕犲犱犻犮犪犾犆狅犾犾犲 犣狌狀 犌狌犻狕犺狅狌563000 犆犺犻 狀犪 犃犫狊狋狉犪犮狋 犮狅犫犪犮狋犲狉犻狌犿狊犿犲 犿犪狋犻狊 isanonpathogenicmycobacteriumcommonlyfoundinnature.Asgeneticengi neeringhasadvanced 狊犿犲 犿犪狋犻狊hasbeen widelyusedinbacteriology virology oncology andparasitology.This reviewsummarizesadvancesinthepreparationofrecombinantM.smegmatisandthestudyofitsimmuneeffects. 犓犲狔狑狅狉犱狊 Geneticengineering 犮狅犫犪犮狋犲狉犻狌犿狊犿犲 犿犪狋犻狊 recombinant review ±Q¸Bacilluscalmetteguerin BCG å J" ê Q b M ¢HâY´¢H3y4589 BCG Wm]yf g·¸¤§;ph£Eµ£Áâ·¸+¤µ¶d´ 0]Ð0Ny¹·FGº£2 BCG 1> á no 3 É0>IJ6BSTº¨þçU?Cy BCG ·¸ECJ v Q¹·0 - @ þ \ nÁè Ì £ 3 g QEC ý ûü â Y 犮狅犫犪犮狋犲狉犻狌犿狊犿犲 犿犪狋犻狊 MS å J" Àè3âY 23EC ¹ :1 > u t IJ çno1lmþÛ¦2_"c@1PÙø IJyZGyz rs@ ¥ _´¹·VW¹· ¹¡ ¿ 56þ¢¹· cd ç ª ]¹· Ûå J"MN y·¸m]Å8Ê®6&ÒfgEF¤µ¶ý!þÿ/MZ Goã MS W£3y5 W ÃÎy ÇÈü ë ¤ C yfg 9Ë MSyH[-¹·º£y*+}oVJ/JKÒW 1.1 ÐìíúJ Sha| ¢Hâ Y Mycobacteri umtuberculosis Mtb H37RvfgË DNA Wèjka§Z bcdPCR de Rv1987 fgjk $ pVV2 e_ sl MS H[rMSRv1987 VCýfDߪaæ\¬e ÆSDSPAGE tST¹·5øcWesternblot âÑO STâã¡13.5×10 Mr ØC Rv1987STIJrMS Rv1987¹· C57BL ³ÁüË¢lme½ TQfIL t¹·óSTIg ®çγ ¦fIFN γ ¹Uô;rMSRv1987þXno³1fg lmTh M¹·d×Huang | H37Rvfg Ë DNA W è de Rv2816c fgjk$ pALACEHis e_sl MS H[rMSRv2816c WesternblotâÑORv2816cSTECyZGÏB ª«avOrMSRv2816czãä1åt±ä1å¥MIC 04ÓÉ!rMSRv2816ct MSVec Ç⻫í THP1·¸ lm °72hÉrMSRv2816cË ·¸ l m C ¨ D 4 ´ Ç Ë( D Rv2816cþ K } ·¸ lm C sCº3GYan| Mtb fg Ë DNA W èPCR de Rv2788 fg!ø_$ pNITmyc e_ sl MS H [ Rv2788k I JyrMSRv2788 É'av¢£OrMSRv2788@į lm-þÒez k ã s © )、 CSDS d Ûß{|Z1f|d¾yZç]6C¨Yang | 10 Mtb fg Ë DNA W èPCR de PE _ PGRS18fgjk$ pALACE e_ sl MS H [rMSPE PGRS18 !ø25ÿ y MOI «í ± ÿ l 6 lmr «í THP1·¸lm´6h 24h 48h 72h £§^_` PCR RTPCR `a $ 24h ÉIL6 IL1 β tIL10 k 4"U4IL12p40k4®çªlmF`aIDrMSþ D8·¸lm±úK}m®CKadir| 11! MtbAg85B À " ZGI L P21 P26 t P53 jkã pNMN064 e_ sl MS H [ rMs064 Western blotâÑO9 Ë ST EC yZG! rMs064 ¹ · · B %CDEFGHI 犑狅狌狉狀犪犾狅 犘犪狋犺狅 犲狀犅犻狅犾狅 犵狔 2018ÅÆ Ç13ÈÇÉ January2018 Vol.13 No.1 9:;#ú]>'E ý ú ] %&( [ § x Í 8ú > Ìç2016 25); ú]%&(%&6'E&(@&1ÐÎ ¸¿Í8ú%'(20162310 ); ú]%&(@&1Ðθ¿9 8ú%201751019 )。 <=>?3W~Email zbyzl01 126.com >?@Ak K1995- ), ØZ à 0ÜÝÞ* +1ßà*+bÙï1«í6¹·Email 302618249 qq.com

DOI 9EصHIŒº ìÿficjpb.org/pdfup/2018021368173209.pdf · 2018-02-13 · P53)jkªpNMN064,e_sl MS,H[rMs064,Western blotâÑŽOł9¸STECœšyZGž。 !rMs 064„

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Page 1: DOI 9EصHIŒº ìÿficjpb.org/pdfup/2018021368173209.pdf · 2018-02-13 · P53)jkªpNMN064,e_sl MS,H[rMs064,Western blotâÑŽOł9¸STECœšyZGž。 !rMs 064„

DOI:10.13350/j.cjpb.180122 ·�/

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犃犱狏犪狀犮犲狊犻狀狉犲狊犲犪狉犮犺狅狀犵犲狀犲狋犻犮犲狀犵犻狀犲犲狉犻狀犵狅犳狉犲犮狅犿犫犻狀犪狀狋犕狔犮狅犫犪犮狋犲狉犻狌犿狊犿犲犵犿犪狋犻狊

LIXiang,ZHOUBiying (犇犲狆犪狉狋犿犲狀狋狅犳犘犪狉犪狊犻狋狅犾狅犵狔,犣狌狀狔犻犕犲犱犻犮犪犾犆狅犾犾犲犵犲,犣狌狀狔犻,犌狌犻狕犺狅狌563000,犆犺犻

狀犪)

【犃犫狊狋狉犪犮狋】 犕狔犮狅犫犪犮狋犲狉犻狌犿狊犿犲犵犿犪狋犻狊isanonpathogenicmycobacteriumcommonlyfoundinnature.Asgeneticengi

neeringhasadvanced,犕.狊犿犲犵犿犪狋犻狊hasbeenwidelyusedinbacteriology,virology,oncology,andparasitology.This

reviewsummarizesadvancesinthepreparationofrecombinantM.smegmatisandthestudyofitsimmuneeffects.

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January2018,Vol.13,No.1