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DOGRISK
• Post docs of the group: – Liisa Uusitalo, PhD (human nutrition), – Sara Zaldiv DVM, Phd (genetics),– Ingrid Hang (DVM; GI-diseases) – Ranno Viitmaa (DVM, Neurology)
• PhD students of the group: – Johanna Roine, MSc(animal breeding and nutrition),– Riitta Seppänen (DVM, dermatology), – Maritsa Palmunen (DVM ; epidemiology), – Liisa Korkalo (Human nutrition, epidemiology).
• Leader: Anna Hielm-Björkman, PhD
DOGRISK: Doing clinical studies, dietintervention studies, epidemiology, FFQ, validating outcome measures, and analyzing biological samples of
phenotyped dogs
Dept. of Equine and Small Animal Medicine - Fac. Vet Med
On the edge clinical studies with … orwithout, human relevance
• Artificial joint implants
• Feacal transplantation
• Epileptic, Neuropathicpain, Osteoarthritismedication/treatments
• New animal sedativepharmacology
• Horse, cow, dog, cat, rabbit, elephant…tohumans!
Food frequency questionnaire
Pros Cons
Long-term intake Memory-dependent
Large study populations Less detailed
Diet-disease associations Amounts crude or unknown
• Principle: List of foods provided, frequencies asked• Web-based, 47 foods, specifications in drop boxes • 5 frequency options (never---daily or almost daily)• Is validated against an other method
• Use in: Case control setup, logistic regression
Pet dogs as an animal model for studying diet-environment-disease associations
Nutritional epidemiology in canine population combines some of the advantages of two research traditions:
Animal experiments Human epidemiology
Short follow-up times Ethical considerations
More monotonous diets Shared environment
Feeding in human control Similar health problems
Phenotyping… with numbers
• Diagnostic tools: Clinical examination, lameness exam, force plate, carpet, scales, x-ray, MRI, CT, thermography…
• Validated indexes: HCPI, F-CSI, neuro (FinFun),… CADESI, CIBDAI, VAS
Taking and using biological samples
• Blood, urine, feces, biopsies, (saliva), hair…
• Creating reference values
• Creating a sample bank, - mostly for own use
• Phenotyped samples
• Time for
anamnesis
Metabolomics• Analyses were done in FIMM
Metabolomics Unit
• Serum samples from 21 dogs and urine samples from 8 dogs (unpublished)
Trace elements, vitamins and fatty acids
• Analyses were done in Mineraalilaboratorio MILA Oy– Trace elements from whole blood (Li-He)– Vitamins A, E, D from serum samples– Fatty acids from EDTA plasma
• Would need: lab for Vit B12, K2, different A,D and E vitamins/provitamins
K R
7.5
8.0
8.5
9.0
9.5
10
.01
0.5
11
.0
Fe
K R
1.4
1.5
1.6
1.7
1.8
1.9
Mg
• 16 RNA samples from skin biopsies havebeen sent to FIMM Sequencing Unit
– RNA sequencing
• qPCR in YES
– RNA from skin biopsies
• To validate the results from RNA sequencing
– RNA from blood samples
• Evaluation of immune response using a candidategene approach
Diet intervention studies in dogs
BEFORE diet intervention (DI):Firmic: p=0.070Fusobact:p=0.086Bacterioidetes: p=0.520
AFTER DI:Firmic: p<0.001**Fusobact:p<0.001**Bacterioidetes: p=0.012*
(n=26)(n=16)
Dogs given an only 100% raw or dry food diet for 4-5 months, before and after DI (unpublished)
Gut microbiota:
Diseases we work with now…
• Osteoarthritis
• Atopy / allergy/ (food sensitivity)
• IBD (~Chron’s disease, Ulcerative colitis)
• Epilepsy
• Hypothyroidism (in dogs)
• Hyperthyroidism (in cats)
• Cancer…
…..(diabetes, heart diseases, lever and kidneydiseases, eye diseases….48)
DOGRISK goes cancer…
• Sniffing dogs sniffing cancer from urine samples
• Together with Wise nose sniffer dog association, East Finland University, HUS – Dept of Oncology and Cancer clinic, Cancer society of Finland, Small innovation firms and start-ups.
• Validating the dogs
• Training for different diseases
• http://www.youtube.com/watch?v=FaK79osKYzs
Ideas…towards innovations…
• New pharmacological or nutraceutical products
• New registered validated outcome measures
• New quick-tests in analyzing
• New apps for canine and human health
• Diagnosing via currents of the body
• Treating via light or a sound
• Mobilizing the inner healing in the body
• E-mail: [email protected]: +358-(0)44-3270462