Fish DNA Barcoding Game!

A Jeopardy-Style Quiz Game

Jeopardy is a trademark of Jeopardy Productions, Inc.

Answer

An organelle other than the

nucleus that contains DNA.

Question

What is a mitochondrion?

Answer

All of the chromosomalDNA found in a cell.

Question

What is genomic DNA?

Answer

The process of rupturinga cell to release its

contents.

Question

What is lysis?

Answer

A suspension that trapsimpurities from fish tissue

and prevents contamination

of DNA.

Question

What is matrix?

Answer

The liquid portion above the pellet

formed after centrifugation.

Question

What is the supernatant?

Answer

Of the four PCR reactionsin your table setup today,

this is the sample thatdoesn’t have any DNA in

it.

Question

What is the negative control?

DAILY

How much do you wish to wager?

DOUBLE

Daily DoubleAnswer

An enzyme used in PCRthat is able to function atvery high temperatures.

Daily DoubleQuestion

What is Taq polymerase?

Answer

These target the portionof DNA to be amplified.

Question

What are primers?

Answer

A step in PCR in which thetemperature is lowered to

50–65ºC, allowing the primers to bind to the

DNA.

Question

What is annealing?

Answer

The complementary strandof the ACTGGTTA templatestrand that is being copied

during extension.

Question

What is TGACCAAT?

Answer

The name of the genethat is being amplified

by PCR in the Fish Barcoding Kit.

Question

What is COI?

Answer

This acts as a sieve toseparate DNA based on

its size when an electricalfield is applied.

Question

What is an agarose gel?

Answer

The size of the amplifiedportion of the COI gene.

Question

What is 650 bp?

Answer

It weighs down theDNA sample so that it

staysin the well after loading.

Question

What is loading dye?

Answer

It’s used to determine the size

of the DNA (in base pairs) in an agarose gel.

Question

What is a DNA ladder ormolecular weight ruler?

Answer

Where negatively chargedDNA migrates to during

gel electrophoresis.

Question

What is the positive oranode side of a gel

electrophoresis chamber?

Answer

P1000P200P50P20

Question

Which of these isnot an actual pipet?

Answer

DNA extractionRNA extraction

PCR Gel electrophoresis

Question

What is RNA extraction?(not part of today’s experiment)

Answer

CodTrout

CatfishMussel

Question

What is mussel?(not likely to work with fish barcoding kit)

Answer

DenaturationAnnealingExtension

Sequencing

Question

What is sequencing?(not a step in PCR)

Answer

Thermal cyclerCathodeAnode

Electrical current

Question

What is a thermal cycler?(not used in gel electrophoresis)

Answer

The identification of anorganism at the species

levelusing a several hundred

base pair sequence of DNA.

Question

What is barcoding?

Answer

The order of bases thatmake up a DNA strand.

Question

What is a sequence?

Answer

The database used toidentify a mystery fishat the species level.

Question

What is BOLD?

Answer

A group of organismscapable of interbreeding

andproducing fertile offspring.

Question

What is a species?

Answer

A place where specimensare stored to support

futurescientific investigations.

Question

What is a biorepository?