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Distributed Sensor Network

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Distributed Sensor Network. Graham Wiley Leonid Sukharnikov. Levels of Abstraction - System. HSL Amplifier. Arabinose Sensor. Levels of Abstraction - Devices. HSL Sender. Arabinose Promoter. HSL Sender/ Reciever. CFP. YFP. Levels of Abstraction - Parts. Expected Output. - PowerPoint PPT Presentation

Text of Distributed Sensor Network

  • Distributed Sensor NetworkGraham WileyLeonid Sukharnikov

  • Arabinose SensorHSL AmplifierLevels of Abstraction - System

  • Arabinose PromoterHSL Sender/RecieverHSL SenderCFPYFPLevels of Abstraction - Devices

  • Levels of Abstraction - Parts

  • Expected OutputExpanding YFP ring surrounding CFP circle shows signal propogationMedia plate w/ bacterial lawn

  • ArabinoseMechanism

  • HSLMechanism

  • HSLHSLMechanism

  • Construction2134 partsFinal

  • Construction Results2134 partsFinal

  • FUTURE WORK

    Check construct via sequencing Reconstruct if necessary

  • Simone MacmilDurga P Sarvepalli

    4 November 2006Detecting small molecule signaling using phosphorylation dependent mechanism in E.coli

  • SMALL MOLECULES Smallest part of pure chemical substance that retains its structure and properties.Play an important role in multiple signaling mechanisms

  • APPLICATIONS OF SMALL MOLECULES IN BIOLOGYSmall molecule drugsBiopolymersSynthetic peptidesPrimers

  • NEED FOR SMALL MOLECULE DETECTIONUnderstand molecule protein interactionsEffect of molecules on the viability of cellsGenetic changes caused by molecules used in molecular therapyDrug discovery

  • CURRENTLY AVAILABLE TECHNIQUESAnalytical: NMR, Western blot, Spectrophotometry, Chromatography, ELISASmall molecule protein interactions : Nanowire sensors ( Wang et al , PNAS 2005;102;3208-3212)Disadvantages: whole cells cannot be usedUsing micro channels whole cells can be used and less amount of reagents requiredMicro channels can also be customized according to the design of the experiment and allows studies to be conducted under flow

  • FABRICATION OF MICROFLUIDIC CHANNELPDMS - Poly dimethyl siloxane

  • MICROFLUIDIC CHANNEL DIMENSIONSChannel width : 200m depth : 50 m

  • DEVICEInduction

  • METHODSTransformationParts from the registryRestriction enzyme digestionSequential ligation to create constructSequence transformantsLigate into pUC Grow selected transformants in micro channels

  • RESULTSAspartate detectionMicrofluidic channelCells grown in Minimal Media lacking aspartate and induced with IPTGCells fluoresce after 30 min in the presence of LB

  • Movement of E.coli in the channelRESULTS

  • FUTURE WORK

    Improvise microchannels

    - Study flow of bacteria under various conditions - chemotaxis, sensitivity of bacteria in the gut to pH, temperature.

    Detect low concentration of small molecules formed during bio-industrial production.

    -Design appropriate sensors for detection (small peptides and sugars)

  • ACKNOWLEDGEMENTSDr. Bruce RoeDr. Matthias NollertDr. David SchmidtkeDr. Fares NazarDr. Randall HewesDoug WhiteSandra Bryant

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