DiscovIR-GC ™

DiscovIR-GC™

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The Epidemic is coming (Tucson News KOLD/KMSB)

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Synthetic Analog Problem DEA Temporary Placement of JWH-018, JWH-073, JWH-200, CP47,497 and CP47,497 C8

homolog, MDPV, Mephedrone, Methylone on Schedule 1

H.R. 1254: Synthetic Drug Control Act of 2011

AK S 17: Classifies certain synthetic cannabinoids as schedule IIA controlled substances.

AL H 158, S 207 and S 208: Relates to schedule I controlled substances; provides legislative findings relating to controlled substance analogs; adds cathinone compounds, certain named chemical compounds of synthetic cannabinoids, and controlled substance analogs to the schedule I controlled substances list subject to regulation and penalties in the same manner as other controlled substances.

AZ S 1043: Relates to dangerous drugs; relates to definition.

AZ H 2388: Adds AM-2201 and JWH-122 to the definition of “dangerous drugs” and to schedule I. Relates to authority to define the chemical composition of controlled substances included in drug schedules; provides for authority to include chemical variations of those substances as necessary; provides consideration of a substance’s potential for abuse, pharmacological effect, current scientific knowledge and the risk to public health; requires written report; exempts the board of pharmacy from rulemaking requirements.

CA A 2420: Provides that every person who possesses, under circumstances that do not include possession for sale as proscribed pursuant to existing law, 28.5 grams or less of any synthetic cannabinoid or any synthetic stimulant compound, or any synthetic stimulant derivative is guilty of an infraction punishable by a specified fine.

HI S 1305: Adds HU-210, JWH-018, JWH-073 to controlled substances provisions.

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Multiple isomers to choose from !!! A cannabinoid Example

Class 1: Classic Cannabinoid Class 2: Basic non classic Cannabinoid Class 3: Naphtoylindole

Class 4: PhenylacetylindoleClass 5: Benzoylindole

(+-)CP 47,497 (1R,3S) or (1S,3R)

CP47,497 Diastereomers and Homologs

JWH-250

JWH-302

JWH-201

DiscovIR-GC

Positional Isomers

JWH 302 vs JWH-201

Analysis of a K-2 Sky Sample

Plant Extract/ Fatty Acids

Identification of JWH-203

JWH-237

JWH-206

JWH-018

JWH-018 1’napthyl isomer

JWH122

AM2201

AM2201 2’napthyl isomer

JWH-098

JWH-210

AM694 4-iodo isomer

JWH-022

JWH-016

JWH-020

JWH-015

JWH-007

JWH-018 adamantyl

RCS-4 C4 homolog

JWH-203

Other Cannabimimetics spectra recently acquired on the DiscovIR-GC

JWH-081

JWH-398

RCS-8

RCS-8 3-methoxy isomer


JWH-200

JWH-200 2’ napthyl isomer

AM-694

AM-694 3-iodo isomer

JWH-251

RCS-4



JWH-073

JWH-073 2’napthyl isomer

JWH-250

JWH-201

JWH-302

DiscovIR-GC™ The Solution for Designer Drugs

Step #1 Separation by Gas Chromatography

Step #3 Chemical Identification - using FTIR specificity (positional and stereoisomers)

Step #2 Controlled Deposition - unique solid phase deposition.

GC typical

How does it work ?

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GC-IR System optics

Polystyrene CalibrationInfrared beam

NIST Traceable polystyrene filmfor daily calibration test Acquisition of solid phase

transmission spectra through ZnSe disc

Disc Track Pointer

End Sample 2 RT= 20.0 min

Start Sample 2 RT= 0.0 min

Records linear positionand rotary position forFor every spectra

Infrared beam fixed in position

ZnSE Disc rotatescounterclockwise

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Sample deposit ZnSe window

Optical path

Vacuum chamber

ABB BomemMCT

Precision Vacuum Backplate

Cryogenic LN2

IR Detector

Interferometer PCB

Heated Transfer line

GC-IR/MS

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Loading the sample disk

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DiscovIR VACUUM CHAMBER, (10-4 torr)

4 inch diameter ZnSe disc, washable & reusable Holds 50 hours of chromatography in spiral track Auto location, Checks for sample, Post dep. analysis

Resistive Heaters +/-5oC

Disc auto load/unload

Sample collection mechanism

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Data Acquisition and Instrument Control software based on Thermo GRAMS™

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Spectrum Trace

Spectrum Trace

Chromatogram Trace

QA/QC / Performance Qualification

Voltage check to ensure detector is working properly

Noise check to ensure proper signal/noise ratio

NIST traceable polystyrene scan is performed

A test mix (amphetamine, phentermine, pseudoephedrine, caffeine, cocaine and alprazolam) enables :

• GC check (proper separation)• IR detector (proper identification)

Example of a Daily Test Mix Chromatogram

Typical Case Work Sample Preparation

Sample preparationSample can be extracted/prepared as if

analyzing via GC/MSMeOH (dilute and shoot type)Basic CHCl3, Basic Hex, Acidic CHCl3Acidic Hex

Normal GC/MS vials can be used

no real change of procedure needs to be employed

Amphetamine mixture Chromatogram

Amphetamine & Methamphetamine

Fingerprint Region

MDA & MDMA

Phentermine & Methamphetamine

Methamphetamine via Shake and Bake method:Analysis of a field sample by GC-IR

CMP

MethamphetaminePseudoephedrine

Diastereomers in Meth Synthesis

Pseudoephedrine

Ephedrine

Pseudoephedrine IR SpectrumDirect Deposition VS. Solid Phase Reference

Comparable To Solid phase/condensed phase IR spectra run with:- ATR- KBR pellet- Nujol Mull

FTIR Specificity: Pseudoephedrine hit list

Emerging Threat 2C-Phenethylamines

Bath salt sample Analysis

4-fluoromethcathinone overlaid with 3-fluoromethcathinone

Solid Phase Mid-IR Spectrum of Mephedrone

Solid Phase Mid-IR Spectrum of Lidocaine

Solid Phase Mid-IR Spectrum of MDPV

Presence of one Cannabinoids: JWH-018

Advantage of Solid Phase DD GC-IR

Sample at RT 21.9 minutesafter 600 scans

Advantage of Solid Phase DD GC-IR

Noise or Split Band?

Rescan enables increases SNR for Decision

JWH-073 and JWH-015 isomers MW 327.4

JWH-073 overlaid with JWH-015 isomer

Library search of JWH-15 versus library of 40 cannabinoids results in quality score of 0.97

Tryptamines

Clandestine Tablets

Most clandestine tablets are mixtures

Can obtain an infrared spectra for each component

With the DB-35 column can separate BZP, TFMPP and MDMA (see figures 4-7)

Isomer determination for MDMA, TFMPP and CPP (to name just a few)

Exhibit containing BZP, TFMPP, MDMA and Caffeine

Clandestine Tablet: Containing BZP, TFMPP, MDMA and Caffeine

Why use DiscovIR-GC?

Resolve and identify mixed analytes in a single instrument and procedure.

GC-FTIR provides two analytical methods in one operation. High resolution solid phase spectra Self-cleaning IR disk. No need to manually clean off old samples. Automatic operation up to 50 hours: Autosampler-compatible. Spectral identification library software. Nanogram sensitivity. Flow splitting: can interface with existing GC–MS instrumentation. Real-time or post-run data collection. Allows re-analysis of

deposited samples

Substances Routinely Analyzed via GCIR system

Methamphetamine (includes clan lab, powder and residues)AmphetamineCodeine cough syrupHeroinMorphine (residues from spoons, straws, syringes etc)CocaineClandestine tabletsOxycodone (residues from spoons, straws, syringes, etc)Tryptamines and phenethylaminesBath Salts Cannabinoids

Documents

DiscovIR-GC ™