Source . bup '"'ww!!llfMCO wPl'btmVI.bfteS! OIedpds. ~;IObfp hml

5210 BIOCHEMICAL OXYGEN DEMAND IBODr

5210 B. 5-Day BOD Tnt 1. a.n.tJIl DI.eulllon

•. Pt~"""'.' The mettIod 00tIIitlt or /IIng wIlh NITlple. 10 lM!l1Iowing, ., Ilrlighl bolJle of the speafIecl

IÎz. and Il1OJbltinog •• the Il*iJ'Jed lempll1Ilul1I /0( 5 d. Disoso/Yed OXV'iIen Is rnusuAlll lnillIIlly Ind Iller Il1OJbllIon.1I'Ill1tM 800" oomponedl'rom lhe clifI"eterlllIl befWr!en lnItiII and I\PIII 00. 8ecIUH the Intill

00 Il del.rrnIntO IhofIly .n.... 1IIe dilllllon Il mIde.lIIlllll)'gell UpIKlI oc.aJrmg illier Ihismel5Uremelllls

lnd~ ln !hl BOO mnSlnmet'il.

II. San,..", lIId PlJ{1II: SImpIIIs lor BOO .n.1ysÎ$ fMy _Ille aignifieantly ôurinO ltotlQI bel' un CCII'Idkln .ncI_ly:sI$. Ie5lJllng ln Iow BOO vllues. Minma. redI.Iction 01 BOO ~ lnI/yljng IImple prompWy or by ooollnglt ID lIIIar·fnlezîng Idi.,e.1Itunl during stol1Ige. fkrwe __•_" kM lempll'ltln.

UIp holding lime ID 1 iRllirTun. Wmn Cl'illed Nl'llpIe$ II) 20 1 3"c belote _lysiI. 1) GniD lII'I1lHS - Il' lnalysis il begun witIlin 2 " 01 çollec;tion, cclcIllGl1lQI .. Ullneeessllty. If .,.Iytb

il nDt Il.rted ....... 2 Il oIlI~e eoIIIelion. kllep $IIllpIII. or b&Iow 4'C 'rom lhe tirnI of CDIII

PfePlre relgents ln """nce but cbcard If ~ Il.-.y sIgn cA IftC4Àllltion Of bioIogie,ll orowth ln the

SIOCIt bottles.. COm!TIer1HI equinlerts cA tI-. "'lIgII'â .... KCIlI:bble ..cIllitf_nt a10ek conc:enlf1Ition

m.y 'De ",S«! il lloses .-e .,.usted PfOPOl1ioM1y.

•• ~ bcItrer soMlion.: omotve $.5" KH~.. 21.7~ Iill(~•. 33.-41il ,....HPO..7~,.nd 1.7

iii NHoCIln .blIllt ~ ml dislilled••MId diIule to 1 L The pH s/IoUld Ile 7.2 wiUIOIA fWÙ8" MljUSlment.

AltemaIlivfIl)', cf 4... Q5 Il KHPQ. or 54.3 Il I(,HPO.ln .bol,II7lll1","~_. AdjUll pH 10 7.2

'lritll ~ NIOH .nd dilute to 1 L

b. AlagrJN.Îl.m ~ 1OhJtiotI. Dis$llIVe 22.5 Il MgSO..7H;:O ln diICiIed ...Ier Md dllllllO 1 L.

1:. ea.tiwn dobldlr MIIl.tiDn: OlSSOlYe 27.5 0 ~ ln ftiIed __ Md dlute 10 1 L

d. ~ cNoIfdIr ~. Ol5soIYI 0.25 Il F~ ln (hliled ....... lInll dllull 10 1 L

1. Add and tIIkaII ~ lN. lof' MWl4iullon of CIUItic or .cid ~e s-mples.

1) Add _ SlDWly while stirrlng, .cid 28 ml oonc tulfllrie .cid 10 distllecl_er. 0IIUle to 1 L

2) ADuII_1JissoIve -40 1l5Odlum lI\Ôwdcle ln dIIlhd ...1.... OlIl.rte to 1 L

f. SOdIiIm wtfë.autJoo: Di1«1M11.575" N~ ln 1llllO ml dlsmed wat~. Thl!l sokMoo III nat

stable: pœPllnt dety.

Il. Mtrî/IctJtiorI ~2-ehIoto-6-(lrichlorometll~ ~ne.·

h, ~ fI(:id aoUiorI. Dry "'egenlll~ gllJOO5e n ref\lelltllrtMn 01l.Un'*: edd" lOS"e !or 111.,I,dd 150 malilkl::oslt.nd 150 ma glLUmle ~ 10 clsliled water.m ôlute 10 1 L F'fegeN frwIl 1mmel:I.."Y beloN lIMI.

~ Amrrlof*m r:NoildlllOldlcn: lJls3

Sten lA ....,-.lllllullon __ for monI tIIa'l 2. h .nef tdding ~ mlrMmIls. .nd buff&r unlllss diutlon

""". blMks COOIlslentlY meelqutlity control imiII;. Oiseard $toted suœ _ter il diIuIion water bIlInk

sI'lOWS more Ih.1'I 0.2 mgIl 00 deplelion ln 5 d.

c. Gll.co~ lQd ctre

2) Seed oonlIOl- Oetiw" .... BOO of \he seedilll maerial as 101' any othet $ample. Tl1Is Is U. SfHId

~. From ua. v",,", of lhe teed c:ontrolend e klla..1 l:ôM of \he Meding matertlll dlullon On ua. dIulIon _I~ deI~ Med DO upteke.lôNItY. melœ dlutlons 01 seed lUth Ih.' Ille I.rgesl q~ resub ln

.1e15l 50'" DO depletiCln. A plol of DO depletion, ln ITIlIgfwns per liler..... l'$IlS mlIIlIeIs 01 seecllor"

bolIIel hmng 1 2-mg/l 'lelllllion and Il.lkrQ/l mininun rflIdlill DO lhoUld pl lM 1 Sll'aIghlIlne for

wIIIch the lIope indicaI.es DO depIellon pel" millier ol SMd. TIle ~ illelœpt Is axygen depIlIIIon

tI\ISoId by the dlküJn waler end shDllId Ile ... Ih.... 0 1 ~ ft 411). Al,"""""" divlde DO depIetIon by velume 01 $Hel in millilers for Iec/I seed coMrOI bollIe having e 2-4!9'L depletlon end e 1.G-lodlne n poirIl for rniduel. Adcllo neutreliHd ~!he rel.-veVOhme 01 ~soIutIon do!t&"iÎIlild by ~ lbovelest, mbl, and e/Ilf 10 10 20 n*l dIlidr.

semple lor reslduer dllortne.(NOTE: F" • Ne~ ""eltS .... 0ll)'Q8f1 dM\II'Id and mets slowIy with

CIfl8ln orverllç eNol.. ,.... toliipounds Illet mly Ile IQ$CInt in chlol1nellld _iipl",) 3) SlImple:s: eortelrq olher IlIllIc substenon· C«1eIn industriel WQtes. forell8lT1Pe. plellng w85tH,

oonteln lOIde meteb. SUctI semples olten requirtl IPtcillltudy end 1tUilmtflt.

4) ~ SlJpetSltutllted oMlh 00 - SamPes toIUIning moN Ihan 1 mg DOi L al 2O"C rnay be

eneourll-.d ln llDld wMefS or ln ...cetwl'lere phoI~ oocu..... To prnent 1055 or 0X'flIl!'I1 dul1ng

lneul*ion 01' such s.amples. reduee 00 10 satul'\I6on" 2O"C II)' brlnging samPie 10 aoout 2O"C in pnaly

a.d tIIIlu...m1le 8lI~8tI~ II)' 'ofIgorous sNIUlg Of II)' MntifIg wilh de8n,lV1erecl eon1PlU5ed er.

.5) S8mpI8lmnpet1ltUflllIdjusIrnerI. BrrQ _'IPM 10 20 '" l·C before rNIking 61utions. e) NItrWIc:a1lotllnhlbtlon -. nIlrtIIc8lion inhibition it lIalfed iIdd 3 mg 2-e1llclro-6-(\riehloIo metllyl)

pyrIdlne (TCMP) 10 eldl3OO-mL boIIllI befOI'\l e.ppng Of iIdd ~ ernomls mlhe 61U1ion ~151 to make a ~I c.onc:el'llratlon 0110 mWL (NOTE. PIA TCMP may dissoMl 5Iowly and cali noal OIlIOP orlha sample. Sorne CGlii""dal fom1ul.IlIorts l;\i&6otve n'lOI'IIl'\Iadlly 1llA .. nollOO'1lo TCMP; acljusl. closaoe M:COIdlrVY.) 5am1lle5 thaI m.y fl!JQ\àflI nitriIIcallon ltIh1bl1on lndude, but .. nol hmiled 10, bioIoglcaly

truted tiIIuenls, Mm'" .alded wiIh bIoIoglc:.Dly!tulfld 8ftklen\$, anc:t river waI'"", NoM lhe lIM 01' l'IIlrugan ln/ibilioI'I iI'I reporting ruuha.

(0I\tl0tI M$~: Mlk. _rII cIlullons of semple Ihet -..il fil...." ln 1 flI$ldUlil 00 or alleasll/T9'L

and. 00 U~. 01 al1eall21'1'O'\.1fter • .5--4lneub8tlol1. fMllliMIonI .. '8ClOi,".... lded UI'IIe:5I IJlpIf!ellCl with • PlIrticUar S8ITlI)le sIlOIWs Ihl! use of • srnaIer number oIl!1ution1 procIuee. al Iell5t Iwo

bottin gImg aeœp_ble m1nmum 00 deplellon 8IId res!du81 ....... A lIlIlR rapkl.nlllysls, sucll •• cao, may b8 00lTIl8I1Kl .,pwdl'''eIy oMlh l!IOO and serve. 'lIl1ide ln "'ldl~ dtulIons. In the absence of pI10r knowIellge, use ltIe 1'*""10111 dilulion5: 0.0 10 1.11... for lIlJOng il'ldUSll1l111""'stes, 1 10 5" for IWW 8nd

settled ........ewater,.510 ~~ for bioIogkaly ttPlad aflbanl, end 2S 10100'1l0 for poIUled ItYMtmllB.

P1epfIre cHutionI eiIher ln 1Ill1l1ual8Cl eytlndiarS Of lIOlumalr'oc: g\a$SWlIfII, am Iht!n trans1er to l!IOO

boftles or prePll'll dii6dl) ln BOO bo«leI. Eilher dlullOIl INUlOll çan be c;ombinld wilh eoy 00

me~ lechniq..... Tlle numller 01 bolIIl!:!. 10 be prepared for elCll llNtion llependl OIltlle 00

l~ and thll'll.mb8r of l'1IflIlçaln ·d!5111lll

WIlen U$I"" ~Id cyInde~ or YOlurnelItc: 18Sk$\0 PfePlIfII dlutillnt, and lfIIIleI'I M,(tiolll Il _l'l, edd seed eflllerdir8ic:lly 10 dilullon Willer or 10 InllMllual ~rd8rs or l\Is.ks berore dlllllon.

5eedlng 0I1ndiYidu8l ctloideiw or.-u awIda a deciniolg raio ol"Mell aD urJ1lIlI8$1IlauYIg dlutlone

'rI rTlICle. 'MIen dilutions 8r1 prepared dinK::IIy if'I800 boIIleI end when ~Inv b I*'n.ry, aôll seed dlreal)' 10 cliIlAIOn WlI\eI" or dinldly 10 the l!IOO bolIleI. \Mlefl. bolIIe 00Ill81ns Il1OI1Ilhan e~ d Iht!

NmPI8 SIller dikI\ion. IlUIrienu m.y b8 1Imit., if'Iltle cliIuIed sample and $Ubsequently leduoe lliologIl;aII actIYIy.1n sudI aemplas. edd tlle nUlI1lnt. mlnel". Ind bulrer sc*IIIonI ri 38 through al difllQl)' l

1000 1 0

. l, m . elearœl~'

r r: sea • d Incubate 10 5 ,20·C. 1 .p on dnutlo rn ,ra nta 0 BOO

aœd co tents WIIh sampi ,dnutlo 01' e'

te for 5 d at 20·0.

th

ne OU, dl

no bubbTes. For d uUo

en uslng

. Calcul 1·0

G

For uctIlest boille mea"'" Ille 2.o.nv'l ~ DO de'pIetIon .-1 Ille I.D-mgIL reslduel DO, celculela BClOo es lolIows:

'Nhan cIlullon w..... IlOt "I~:

0, • DO Of dlllllld .-npIe lmmedlately llfIlH" prepltetlon, rngIl,

D.l- DO cl dlluted ..m~ aner Sd lneubatlon el 2O"C, rr9'\-, P - cIeclr'Nl vollM'llellk fnK::l1on Of 5lImpie used,

e, - 00 Of seed oon1rol betore Incubllllon, mgA.. (14d), ~. DO cl M«I eottrol eIl~ IneubliltlDn mgA..114dl, eoo

, - ratio 01 ued ln dik.ted ~a 10 lelld in ued control- {'At ued in diklled NmPe)/("lt Mad ln &Md

""""'" '16'IId ~. edded dÎlec:tIJ 10 semple «10 ued eontn>l boilles: ,. (Volume cl eMd ln dîIultd NrTll*)/(Yokme cl Mec! in ued COllltOl)

AiIpOfl r.-ullS .. CSiOO, If nitriflcetion ~ Inhibhd.

'_bn _ umple dlulion l'I'IMIS Ille CIlefla Of a residual 00 Of al 1e15l1 mg/\. end 1 00

Cle9letlol. Of li! leesl: 2 "9'l endltHtte is no ~ Of loxlcity li! ~Ilet $ample c:onoent~« lhe exilIlence Of en otMous enomlIPf, _ age resulls ln the lIDClIlptebIe range.

ln Ihfle ceIculeIIons, do IlOt mile UM.edIoi.s for 00 upteke by lhe dIuIIon -œr Ill.nt du~ng Ino.lbelion. TIwe COIftIdlon bi unlMlœssery • dilIAIon _ter meefs!he blInk a'ilelte SlIDUllled aboYa. If!he

dllr..tlon wM'" doM IlOt meeI. lhe5e aùIriI, IIIOl)er UM.ections are dil'lk:ult : do IlOt reeonl resulls Of, es a

mlnlmum, merIt. \lIem as not meetirQ

AYet'Ige monill/y tindalll deYI8tion: IO.4/f9\.

III a _ of ÎIUII18tlOl'IIlSlIldin,' ellCh IlMIImg 2 to 112 lllbOlaloriel (and 85 many ..,alysts and

seed !IOO~, 5-d 800 trlNSIJremenll were lMlle on synlh~ic Wlltr .."pel OOllI"ni'lg 1 1:1 l!'tI4ufe of gIllOOMlnd gloMmle Iâd ln thl104ll concent~renge of 3.3 10 231 mgJI.. The regresslon eqUlliDnI

for mun vllue, X; Ind $laIldard deIIIItion, S, from Ir- Sludiel ...~

x- 0.&.S8 (1dUed lev&\. mw'lJ. 0.280 mgIL S- 0.100 {8dded Ievel, mwLl • 0.547 mgIL

For lIM! ~ milled PI1marY 5llIndard. the ....... 5-d 80D WlMl be 198 mgIl with IIlIndIIll dtr.1111oo of 30.5 mg,IL \MIen ~ inhibitDn''' lIMd, GGA ln resuItI fallinog outIide the 188.

30.5 œnllOllnjl qUle oIlen IndJc:lle use of lncomra _!'Its 01 s.eeo. Adjust IITIOUnt of MId Idl*llo Ille GOA tut 10 Kt1IIMIlMIJb IlIlrG Mllin I/lÎS t1I,..

a. ~ 1I1W$; Beeause of IN", laaors Ifredlng 900 lests ln mulIllbolMOly MuClie$1IId I/Ie

resuIllng exil"... variabillty in telIl resulls. 0lIl standard devIItIon, as delll .. iÎiMid by Intll1ootloi'1ltol'y lestS.

le I.oonlne..dtd. 1 oontroIlimlI fat lndIvlduIlliobiHllviltts. AlIl1'llliwly, fol' .1dI1lbclrMllC'1, estlbllsh Ils

COl'W1llIImltI by petfon'nlng 1 mîntTun 01 25 gI~ut.am;ç acid c:hIcb ('( "c) __ a period of seVItIl

we.... or lTICll'Il'-lIId e.....4l1ing Ule meln Ind slIndard ~on. usa tI'llI meln '" 3 s1andM'll deYtIlIonI IilIllle COI'bOI'" forfUtln lib' ' 1I11Umk: lÔIl dledlI. eœ c:IIlcl.Q1td COl'W1llIIrrils lo the slngIolaboflltory lests ~ed Ibove Ind 10 1nIl1f1aboiwlOi, 00l"Ilnll1L.mIrieIn Will.... WorkI As5oclIlIon, W~ ErMroomenl FIdII1IIÎOfI.

plJtlclel

•