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The Thermo Scientific™ Dionex™ SolEx™ Solid-Phase Extraction (SPE) Cartridge line of sample pretreatment products includes silica-based and polymeric-based stationary phase chemistries in barrel, disposable cartridge format.

The barrel-format cartridges are designed for compatibility with the Thermo Scientific™ Dionex™ AutoTrace™ 280 SPE instrument for large sample volume applications as well as small-volume applications with vacuum SPE manifolds. Solid-phase extraction (SPE) is a widely used sample preparation method for the concentration of pesticides, herbicides, hydrocarbons, and other organic contaminants from aqueous samples. The Dionex AutoTrace 280 SPE instrument is a high-throughput workstation dedicated specifically for automating SPE with demonstrated use for U.S. EPA methods 505, 506, 507, 521, 525.2, 525.3, 535, 547, 608,625, 1613, 1664, and 1694. The Dionex AutoTrace 280 SPE instrument automates cartridge or disk conditioning, sample loading, rinsing, and eluting steps for large volume aqueous solution extractions (20 mL to 4 L). The unit can process up to six samples in parallel, automatically conditioning, washing, and eluting SPE cartridges or disks with a choice of up to five reagents. The unit also segregates aqueous and solvent waste using separate waste lines for efficient, cost effective waste handling.

The Dionex SolEx SPE line of barrel- format sample pretreatment cartridges are designed for compatibility with the Dionex AutoTrace 280 SPE instrument for large sample volume applications. Large sample volumes are necessary when the required detection limits of the targets are below that achieved by direct injection or the pre-concentration of less than about 20 mLs of sample. The barrel cartridges have luer-slip outlets that are compatible with the 1 mL, 3 mL and 6 mL volumes plunger assembly of the Dionex AutoTrace 280 SPE instrument and include silica-based and polymeric-based stationary phase chemistries.

Thermo Scientific Dionex SolEx SPE Cartridges

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Cartridge Type Format Functional Group Example Applications

HRPHS 3, 6 mL barrelsDivinylbenzene (DVB)/ polyvinylpyrrolidone (PVP, high molecular weight)

General reversed phase, polyphenolics, azo compounds

SAX 3, 6 mL barrels DVB-quaternary ammonium Ionizable (weak) anions, carboxylic acids

SCX 3, 6 mL barrels DVB-sulfonate Ionizable (weak) cations, amines

WAX 3, 6 mL barrels DVB-aminePermanent anions, inorganic and organic, sulfonates, sulfates

WCX 3, 6 mL barrels DVB-carboxylatePermanent cations, inorganic and organic, quaternary ammonium

HRPOnline cartridge and RSLC column

DVB-PVP (low) General neutral organics

C8, C18 1, 3, 6 mL barrels Bonded silica Pesticides, herbicides, hydrocarbons

Phthalate-free C8, C18 6 mL barrel Bonded silica Trace analysis

Silica 6 mL barrel Acid-washed unbonded silica Polar compounds

Activated Carbon 6 mL barrel Charcoal Nitrosamines, 1,4-dioxane

Graphitized Carbon 6 mL barrel Graphitized carbon particles Herbicides

C18 SPE Cartridges for EPA Method 525.2These SPE cartridges are designed specifically for extraction of semivolatiles from drinking water (US EPA Method 525.2). These C18 cartridges are available in a 6 mL cartridge and have 1.5 g of packing.

Unbonded Silica (Acid Washed) SPE CartridgesThe unbonded silica SPE cartridges can be used to remove polar impurities and purify polar compounds. They are available in 6 mL cartridges with 0.5 g of packing.

The decision to choose polymeric or silica-based stationary phase products for SPE is generally guided by pH requirements and relative retention of targets and matrix on the phase. Bonded silica products have a safe operating range generally pH 3–8. The bonded silica phases are generally non-aromatic which means that highly aromatic targets can be more easily eluted with organic solvents. Highly polar targets are generally not well-retained and selectivity among species containing aromatic rings can be limited. These silica phases are generally not mixed-mode.

Polymeric stationary phases have a useful pH range of 0–14. The substrate particles in the polymeric Dionex SolEx SPE cartridge products are based on divinylbenzene and therefore afford retention via adsorption and pi-pi interactions in addition to the surface graft functionality, as reflected in the product name. Dionex SolEx SPE cartridges are offered in three forms: silica-based, which includes various C8 and C18 chemistries and unbound silica; carbon-based, which includes activated carbon (charcoal) and graphitized carbon; and polymeric stationary phases.

Table 1. Summary of the Dionex SolEx SPE cartridge product line with associated chemistries and recommended applications.

Silica-Based SPE CartridgesRegular C8 and C18 SPE CartridgesThe 1 mL cartridges contain 0.1 g of packing; the 3 mL cartridges contain 0.5 g of packing; and the 6 mL cartridges contain 1 g of packing. The C18 material is available as endcapped or unencapped silica to provide the selectivity required by various applications. The C8 and C18 chemistries are two of the most commonly used sorbent types for off-line SPE and are used for concentration of pesticides, herbicides, hydrocarbons, and other organic contaminants from water as approved by the U.S. EPA.

Phthalate-Free C8 and C18 SPE CartridgesPhthalate-free SPE cartridges can be used for extraction of pesticides from water, and are excellent for trace analysis. They are available in C18 and C8 functionalities in a 6 mL cartridge. The C18 cartridges have 1 g of packing and the C8 cartridges have 0.5 g of packing. These have the same specifications as the standard C8 and C18 cartridges, except they feature Teflon® frits and are packed in mylar bags to minimize phthalate contamination.

3Carbon-Based SPE CartridgesActivated Carbon (Charcoal) SPE Cartridges for EPA Methods 521 and 522The activated carbon or charcoal SPE cartridges can be used for the extraction of nitrosamines in drinking water (U.S. EPA Method 521) and for extraction of 1,4-dioxane in drinking water (US EPA Method 522). They are available in 6 mL cartridges with 2 g of packing.

Graphitized Carbon SPE Cartridges for EPA Method 535The graphitized carbon SPE cartridges can be used for the extraction of herbicides from drinking water (EPA Method 535). They are available in 6 mL cartridges with 0.5 g of packing.

Polymer-Based SPE CartridgesThe polymeric stationary phases are built on a new, high-capacity base particle, with a narrow size distribution yielding high efficiency with low backpressure.

SAX SPE Cartridges The SAX stationary phase is comprised of a 22 µm high surface area divinylbenzene resin particle grafted with an aromatic quaternary ammonium functionality. The ion-exchange capacity is 900 µEq/g and the sites bear a permanent charge. This phase is recommended for the SPE of weak acid anions such as carboxylates. The phase is conditioned to a pH at which the target analytes are charged (about 2 pH units above the pKa). The sample is loaded, the phase washed, and the targets are eluted with solvent and at the pH about 2 pH units below the pKa. The target analytes are eluted as neutral species by reversed phase.

WAX SPE Cartridges The WAX stationary phase is comprised of a 22 µm medium surface area divinylbenzene resin particle grafted with an amine functionality. The ion-exchange capacity is about 1200 µEq/g. This phase is mixed mode reversed phase and anion exchange. Strong acids are retained at low pH where the amine sites on the resin are charged and therefore capable of ion-exchange, and eluted raising the pH and therefore neutralizing the stationary phase. This is the common methodology when the target species are permanently (strongly) charged anions such as sulfonates.

Figure 1. Determination of LAS by chain length after pre-concentration from wastewater using Dionex SolEx WAX SPE cartridge with the Dionex AutoTrace 280 SPE instrument.

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SCX SPE Cartridges The SCX stationary phase is comprised of a 22 µm high surface area divinylbenzene resin particle grafted with aromatic sulfonic acid groups. The ion-exchange capacity is 700 µEq/g and the phase is mixed mode with both reversed phase and cation exchange functionality. This phase is recommended for the SPE of weak cations such as weak base amines. The strong cation exchange (SCX) phase bears sulfonate (SO

3-) sites which means it can exchange cations

down to a pH <1.

4 WCX SPE Cartridges The WCX stationary phase is comprised of a 22 µm medium surface area divinylbenzene resin particle grafted with carboxylate functional groups. The ion-exchange capacity is 1000 µEq/g and the pKa of the weak acid sites is about 4. This phase is recommended for the solid phase extraction of strong (permanently charged) cations such as quaternary ammonium compounds. The phase is conditioned at pH 8 or higher. The phase is washed and the targets are eluted by protonating the stationary phase to carboxylic acid (neutral) thus eliminating the ion-exchange retention mechanism. For some applications recoveries can be improved by adding a Soak-to-Elute step before the Elute step in the method. The resin is shipped in the ammonium acetate form. In contrast to the SCX, the weak cation exchange (WCX) phase bears carboxylate sites which have pKa~4. This means that the WCX phase is neutral and will not hold cations at pH<3. Of course, a weak ion-exchange site is 95% charged about 2 pH units from the pKa, so there is some capacity in the proximity of the pKa.

The ion-exchange phases are characterized by their ion-exchange capacities, which allow the calculation of how much sample can be held on the phase. For example, if a phase contains 1000 µEq/g of capacity and the cartridge contains 100 mg of resin, then the cartridge contains 100 µEq of capacity. If a sample contains 500 µg/mL of sodium, for example, the question might be, how many milliliters of sample can be loaded on the cartridge before the resin bed is consumed. 50 µg/mL sodium is 2.2 µEq/mL, so the 100 µEq cartridge capacity / 2.2 µEq/mL sample matrix concentration = 45 mL sample can be processed.

Hydrophilic Reversed Phase Resin (HRPHS) SPE Cartridges The HRPHS stationary phase is a neutral resin comprised of a 22 µm, high-surface area divinylbenzene base particle grafted with polyvinylpyrrolidone polymer. This material has properties of a hydrophilic reversed phase material and also has specific high-capacity for polyphenolics including humic acids, and azo-containing substances such as azo-dyes. The hydrophilic reversed phase properties allow high recovery of hydrophobic targets.

Military installations and armament manufacturers have experienced closures for the last several years due to the non-proliferation treaties and disarmament agreements. These closures have resulted in munitions being disposed off through combustion, thus causing major environmental problems in the soil and groundwater quality. Surrounding land and groundwater have been found to be laden with explosive residues such as 2, 4, 6-trinitrotoluene (TNT) and associated nitroamine impurities. Figure 3 shows the separation of 14 explosives that are currently a health concern.

Figure 3. Separation of 14 explosives in EPA 8330 on Acclaim Explosives E2 Column and Dionex SolEx HRPHS and the Dionex AutoTrace 280 SPE instrument for automated sample preparation.

Figure 2. Determination of triclosan in Sunnyvale, CA tap water after pre-concentration using Dionex SolEx HRPHS SPE cartridges with the Dionex AutoTrace 280 SPE instrument.

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Column: Thermo Scientific™ Acclaim™ Explosives E2, 3 µm 3.0 × 250 mmMobile phase: 48/52 v/v MeOH/H2OTemperature: 25 °CFlow rate: 0.3 mL/minInj. volume: 5 µLDetection: UV at 254 nm

Peaks: (EPA 8330 mix, 2 ppm each) 1. HMX 2. RDX 3. 1,3,5-trinitrobenzene 4. 1,3-Dinitrobenzene 5. Nitrobenenze 6. 2,4,6-trinitrobenzene 7. Tetryl 8. 2,6-Dinitrotoluene 9. 2,4-Dinitrotoluene 10. 2-Nitrotoluene 11. 4-Nitrotoluene 12. 3-Nitrotoluene 13. 4-Amino-2,6-Dinitrotoluene 14. 2-Amino-4,6-Dinitrotoluene

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5SPECIFICATIONS

Silica-Based SPE Cartridges

Regular C8 and C18 SPE Cartridges

Particle Size: 40–63 μm

Pore Volume: 0.70–0.85 cm3/g

Surface Area: 470–530 m2/g

Shelf Life: 24 months

Unbonded Silica (Acid Washed) SPE Cartridges

Particle Size: 40–63 μm

Pore Size: 60 Å

Surface Area: 500 m2/g

Shelf Life: 24 months

Carbon-Based SPE Cartridges

Activated Carbon

Sorbent Material: Activated carbon (charcoal) particles

Surface Area: 972 m2/g

Shelf Life: 5 years

Graphitized Carbon

Sorbent Material: Nonporous graphitized carbon particles

Surface Area: 90 m2/g

Shelf Life: 5 years

Polymer-Based SPE Cartridges

SAX SPE Cartridges

Sorbent Material: Styrene-Divinylbenzne/quaternary ammonium, chloride-form

Ion-Exchange Capacity: 0.9 mEq/g

Particle Size: 22 μm

Surface Area: 800 m2/g

Shelf Life 5 years

WAX SPE Cartridges

Sorbent Material: Styrene-Divinylbenzene/amine, ammonium acetate-form

Ion-Exchange Capacity: 1.2 mEq/g

Particle Size: 22 μm

Surface Area: 800 m2/g

Shelf Life: 5 years

SCX SPE Cartridges

Sorbent Material: Styrene-Divinylbenzne/sulfonate, sodium-form

Ion-Exchange Capacity: 0.7 mEq/g

Particle Size: 22 μm

Surface Area: 800 m2/g

Shelf Life: 5 years

WCX SPE Cartridges

Sorbent Material: Styrene-Divinylbenzene/carboxylate, ammonium-form

Ion-Exchange Capacity: 1.2 mEq/g

Particle Size: 22 μm

Surface Area: 450 m2/g

Shelf Life: 5 years

HRPHS SPE Cartridges

Sorbent Material: Styrene-Divinylbenzene/Polyvinylpyrrolidone

Particle Size: 22 μm

Surface Area: 800 m2/g

Shelf Life: 5 years

6 Ordering InformationIn the U.S., call (800) 346-6390 or contact the Thermo Fisher Scientific Regional Office nearest you. Outside the U.S., order through your local Thermo Fisher Scientific office or distributor. Refer to the following part numbers.

Polymer-Based SPE Cartridges Part Number

HRPHS SPE Cartridges

Dionex SolEx HRPHS 3 mL Cartridge with 60 mg Resin, Package of 48 088124

Dionex SolEx HRPHS 3 mL Cartridge with 150 mg Resin, Package of 48 088125

Dionex SolEx HRPHS 6 mL Cartridge with 100 mg Resin, Package of 48 088126

Dionex SolEx HRPHS 6 mL Cartridge with 200 mg Resin, Package of 48 088127

Dionex SolEx HRPHS 6 mL Cartridge with 500 mg Resin, Package of 36 088128

SCX SPE Cartridges

Dionex SolEx SCX 3 mL Cartridge with 60 mg Resin, Package of 48 088097

Dionex SolEx SCX 3 mL Cartridge with 150 mg Resin, Package of 48 088099

Dionex SolEx SCX 6 mL Cartridge with 100 mg Resin, Package of 48 088101

Dionex SolEx SCX 6 mL Cartridge with 200 mg Resin, Package of 48 088102

Dionex SolEx SCX 6 mL Cartridge with 500 mg Resin, Package of 36 088103

SAX SPE Cartridges

Dionex SolEx SAX 3 mL Cartridge with 60 mg Resin, Package of 48 088105

Dionex SolEx SAX 3 mL Cartridge with 150 mg Resin, Package of 48 088106

Dionex SolEx SAX 6 mL Cartridge with 100 mg Resin, Package of 48 088107

Dionex SolEx SAX 6 mL Cartridge with 200 mg Resin, Package of 48 088108

Dionex SolEx SAX 6 mL Cartridge with 500 mg Resin, Package of 36 088109

WAX SPE Cartridges

Dionex SolEx WAX 3 mL Cartridge with 60 mg Resin, Package of 48 088111

Dionex SolEx WAX 3 mL Cartridge with 150 mg Resin, Package of 48 088112

Dionex SolEx WAX 6 mL Cartridge with 100 mg Resin, Package of 48 088113

Dionex SolEx WAX 6 mL Cartridge with 200 mg Resin, Package of 48 088114

Dionex SolEx WAX 6 mL Cartridge with 500 mg Resin, Package of 36 088115

WCX SPE Cartridges

Dionex SolEx WCX 3 mL Cartridge with 60 mg Resin, Package of 48 088117

Dionex SolEx WCX 3 mL Cartridge with 150 mg Resin, Package of 48 088118

Dionex SolEx WCX 6 mL Cartridge with 100 mg Resin, Package of 48 088119

Dionex SolEx WCX 6 mL Cartridge with 200 mg Resin, Package of 48 088121

Dionex SolEx WCX 6 mL Cartridge with 500 mg Resin, Package of 36 088122

Silica-Based SPE Cartridges Part Number

Regular C18 SPE Cartridges

Dionex SolEx C18 6 mL Cartridge with 1 g of Resin, Package of 30 074410

Dionex SolEx C18 6 mL Cartridge with 0.5 g of Resin, Package of 50 074417

Dionex SolEx C18 3 mL Cartridge with 0.5 g of Resin, Package of 50 074412

Dionex SolEx C18 1 mL Cartridge with 0.1 g of Resin, Package of 100 074623

Dionex SolEx C18 Cartridge (un-endcapped material) 074416

Regular C8 SPE Cartridges

Dionex SolEx C8 6 mL Cartridge with 1.0 g of Resin, Package of 30 074416

Dionex SolEx C8 6 mL Cartridge with 1 g of Resin, Package of 30 074411

Dionex SolEx C8 3 mL Cartridge with 0.5 g of Resin, Package of 50 074413

Dionex SolEx C8 1 mL Cartridge with 0.1 g of Resin, Package of 100 074415

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sPhthalate-Free C8 and C18 SPE Cartridges

Dionex SolEx C18 Clean 6 mL Cartridge with 1 g of Resin, Package of 30 075895

Dionex SolEx C8 Clean 6 mL Cartridge with 0.5 g of Resin, Package of 50 075897

C18 SPE Cartridges for EPA Method 525.2

Dionex SolEx C18 525 6 mL Cartridge with 1.5 g of Resin, Package of 30 075896

Unbonded Silica (Acid Washed) SPE Cartridges

Dionex SolEx Silica 6 mL Cartridge with 0.5 g of Resin, Package of 50 074589

Carbon-Based SPE Cartridges

Activated Carbon (Charcoal) SPE Cartridges for EPA Methods 521 and 522

Dionex SolEx Carbon 6 mL Cartridge with 2 g of Resin, Package of 20 074590

Graphitized Carbon SPE Cartridges for EPA Method 535

Dionex SolEx GCB 6 mL Cartridge with 0.5 g of Resin, Package of 30 075898

PS70836-EN 08/15S

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