Dimensional AnalysisDimensional AnalysisUnits of measures commonly Units of measures commonly

encountered in the labencountered in the lab

Dimensional AnalysisDimensional AnalysisUnits of measures commonly Units of measures commonly

encountered in the labencountered in the lab

gmMng/l

femtogram

From Chapter 13: Seidman and From Chapter 13: Seidman and MooreMoore

I.A. ExponentsI.A. Exponents I.B. Scientific NotationI.B. Scientific Notation II.A. Common logarithimsII.A. Common logarithims II.B. AntilogarithimsII.B. Antilogarithims II.C. Natural logarithims – skip for nowII.C. Natural logarithims – skip for now II. D. pHII. D. pH III. UnitsIII. Units IV.A. EquationsIV.A. Equations IV.B. Units and Mathematical OperationsIV.B. Units and Mathematical Operations

Most Commonly Encountered UnitsMost Commonly Encountered Units

m (meter) g (gram) l (liter)

PrefixesPrefixesm (meter) g (gram) l (liter)

103 mm 103 mg 103 ml

106 m 106 g 106 l

109 nm 109 ng

1012 pg

1015 pg

Use prefixes in tables and graphs

Most Commonly Encountered UnitsMost Commonly Encountered Units

m (meter) g (gram) l (liter)

103 mm 103 mg 103 ml

106 m 106 g 106 l

109 nm 109 ng

1012 pg

1015 fg

Mass of protein, cells Volume of solutions

Weight of single cell

Wavelength of light Size of virus

Mass of DNA

Mass of DNA Volume of DNA or Enzyme

Size of cells

Derived Units - ConcentrationDerived Units - Concentration

g/l = mg/ml = g/l = ng/nl

Derived Units - ConcentrationDerived Units - Concentration

g/l = mg/ml = g/l = ng/nl

103 mg/l = 103 g/ml = 103 ng/l

Derived Units - ConcentrationDerived Units - Concentration

g/l = mg/ml = g/l = ng/nl

103 mg/l = 103 g/ml = 103 ng/l [DNA]

[DNA][Pro]

ppm

Derived Units - ConcentrationDerived Units - Concentration

g/l g/mol

=mol/l(M)

Molar ConcentrationsMolar Concentrations

g/l = mg/ml = g/l = ng/nl___ g/mol mg/mmol g/mol ng/nmol

=? =? =? =?

Molar ConcentrationsMolar Concentrations

g/l = mg/ml = g/l = ng/nl g/mol mg/mmol g/mol ng/nmol

= mol/l (moles per liter) = M (molar concentration)

mMmM

millimolar – units mmol/l – symbol mM

M x 103 eg. 0.05 M = 50 mM

Concentration of buffers (Tris, Phosphate) and other salts (EDTA) in solutions

MM

micromolar – units mol/l – symbol M

mM x 103 eg. 0.5 mM = 500 M

Concentration of trace minerals and vitamins

KM of many enzymes