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Page 1: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

Did you know thatelephants are one of thefew animals that canrecognize themselves ina mirror? And that...

Page 2: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

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Page 3: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

This annual international research prize recognizes

accomplishments in neurobiology research based on

methods of molecular and cell biology. The winner and

finalists are selected by a committee of independent

scientists, chaired by the Editor-in-Chief of Science.

Past winners include postdoctoral scholars and

assistant professors.

To be eligible, you must be 35 years of age or younger.

If you´re selected as this year´s winner, you will receive

$25,000, have your work published in the prestigious

journal Science and be invited to visit Eppendorf in

Hamburg, Germany.

Deadline for entries

June 15, 2008

2007 Winner

Rachel Wilson, Ph.D.

Assistant Professor

of Neurobiology

Harvard Medical School

Eppendorf & Science Prize for

Neurobiology

For more information

www.eppendorf.com/prize

Get recognized!

$25,000

Prize

Page 4: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

GE Healthcare Bio-Sciences AB, a General Electric Company.

Björkgatan 30, 751 84 Uppsala, Sweden

© 2008 General Electric Company – All rights reserved.

GE31-07

Any scale. Anytime. Anywhere.Proven technology for rapid vaccine production.

Pandemics can occur at any time, and the key to successful vaccine processing is to be

prepared for unexpected challenges. GE Healthcare’s comprehensive vaccine solution

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breaking ReadyToProcess™ disposable products, which eliminate cleaning and validation

to significantly reduce process development time.

Through flexible solutions backed by our expert support and worldwide presence we’re

helping you transform healthcare. We call it BioProcess Re-imagined.

www.gelifesciences.com/bioprocess

GE Healthcare

Page 5: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

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Page 6: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

In every successfulpaper you’ll find abeginning, middleand

© Copyright 2007 Thomson. EndNote is a registered trademark of Thomson. All trademarks are the property of their respective companies.

Download your free demo or buy online todaywww.endnote.com

Anyone who has ever written a paper knows all too wellthe steps required for it to become a success. That’s whereEndNote® comes to the rescue. EndNote is there as youstart your research and collect references.And, it continuesto support you as you cite references in your paper.By listening to writers and researchers like you, anddelivering ways to simplify your work, EndNote remainsthe favored, time-saving solution for organizing referencesand creating instant bibliographies. The newest release,EndNote X1, allows you to create custom groups of

references using a simple drag and drop action, manageassociated files with your references easily and more.EndNote X1 libraries can be shared across Windows®

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Page 7: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

To order yours, go to www.EpiBio.com

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Comparison of PCR sensitivity using templates purified with the MasterPure™ Complete

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Page 8: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

the leader in enzyme technology

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ELECTROCOMPETENT STRAINSNOW AVAILABLE

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Cloning strain characteristics Strain NEB #

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Added control of IPTG induced NEB Express l q Competent E. coli C3037H/I

expression with non-T7 plasmids

Most popular T7 protein expression strain T7 Express Competent E. coli C2566H/I

Reduced basal expression T7 Express l q Competent E. coli C3016H/I

Tight control of protein expression T7 Express lysY Competent E. coli C3010H/I

by inhibition of T7 RNA Polymerase

Highest level of protein expression control T7 Express lysY/l q Competent E. coli C3013H/I

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Advantages:

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At NEB, we make transformation as simple as selecting the best strain for your

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For a copy of our new Competent Cell Brochure, please visit www.neb.com/literaturerequest

Page 9: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

The GairdnerFoundationannounces the2008 GairdnerInternationalAwardees.

The Gairdner Foundation is pleased to

announce the winners of the 2008 Gairdner

International Awards. They will be presented at

a gala banquet in Toronto on October 23, 2008.

The Gairdners were founded 49 years ago by

the late Toronto businessman, James Gairdner,

and today rank among the most prestigious

awards in science. Some 70 of 288 Gairdner

awardees have gone on to win the Nobel Prize.

The closing date for nominations for the

2009 Canada Gairdner International Awards is

October 1, 2008. For more information on the

Gairdner Foundation and its national program

of events, log on to www.gairdner.org.

In association with:

Professor Harald zur Hausen, DSc., MDProfessor Emeritus and recent Chairman and

Scientific Director, German Cancer Research

Centre, Heidelberg, Germany

“for his discovery of the causative role of

papillomaviruses in cancer of the cervix

which led to the development of a successful

HPV vaccine.”

Dr. Victor Ambros, PhDProfessor, Department of Molecular Medicine,

University of Massachusetts Medical School,

MA, USA

“for their discovery of microRNA’s and

their mechanism of gene regulation.”

Dr. Gary Ruvkun, PhDProfessor of Genetics, Harvard Medical

School, Boston, MA, USA

Dr. Nahum Sonenberg, PhDProfessor, Department of Biochemistry and

McGill Cancer Centre, McGill University,

Montreal, QC, Canada

“for his discovery of mechanisms controlling

gene expression in human cells at the level

of protein synthesis.”

Dr. Samuel Weiss, PhDProfessor of Cell Biology and Anatomy and

Pharmacology and Therapeutics, University

of Calgary, Calgary, AB, Canada

“for his seminal discovery of adult neural

stem cells in the mammalian brain and

it’s importance in nerve cell regeneration.”

Dr. Allan Bernstein, OC, PhDExecutive Director, Global HIV Vaccine

Enterprise, New York, USA

“for his outstanding contribution to Canadian

health research as a scientist, research institute

director and as the inaugural President of the

Canadian Institutes of Health Research.”

2008 Gairdner Wightman Award

for outstanding leadership in

Canadian medicine

Page 10: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start

polymerase on the market. Phire DNA Polymerase is an ideal solution

for routine and high throughput PCR applications.

Short protocols: Amplify 3 times faster than with hot start Taq

Specific products: Unique hot start technology with zero-time reactivation

High yields: Efficient PCR results in more product

For more information visit www.finnzymes.comTo receive a sample please contact your local distributor.

Phire™ Hot Start DNA Polymerase– speed and specificity for PCR

TOOLS FOR MOLECULAR BIOLOGY

Phire is a trademark of Finnzymes Oy.

Distributed in the US and Canada by New England Biolabs.

Other countries visit www.finnzymes.com.

Finnzymes’

Phire Hot Start

Hot start Taq DNA polymerases

Chemically modified Antibody-based

39 min 80 min 82 min 69 min 100 min

Abundant yields in shorter time. A 1.0 kb fragment from the human glutathione

peroxidase gene was amplified with five different hot start DNA polymerases

according to suppliers’ recommendations. After PCR, duplicates of each reaction

were run on a 1 % agarose gel. Total cycling times indicated at bottom.

You think Taq is hot? This is pure

Phire™

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Page 11: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

Better and Faster Systemsfor Protein Analysis

©2008 Promega Corporation, 16134-AD-GN

Now get enhanced digestion of even the most

difficult proteins and more complete peptide

recovery from gels. The combination of Promega

Trypsin Gold and ProteasMAX™ Surfactant gives you

maximum protein sequence coverage and increased

confidence of correct protein identification.

Express proteins in about one hour, not days,

and use them immediately with Promega

TNT® Systems. Unlike traditional E. coli-based

methods, the eukaryotic-based, cell-free TNT

System greatly enhances consistent expression

of functional proteins.

Learn more about utilizingcell-free expression tocharacterize proteininteractions in ourProtein Interaction Guide.

To request additional information and qualifyfor a free sample of the TNT® T7 Quick System,visit: www.promega.com/proteinanalysis

LeacellchaintPr

To request additiona

Page 12: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

Panel A: Current standard: in-gel digestion of 47kDa protein band

from mouse cytoplasmic protein extract using Trypsin Gold.

Panel B: More complete in-gel digestion of 47kDa protein band

from mouse cytoplasmic protein extract using Trypsin Gold

with ProteasMAX™ Surfactant.

Page 13: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

Established and presented by:

Established in 1995, the GE & Science Prize for Young Life Scientists

brings science to life by recognizing outstanding Ph.D.s from

around the world and rewarding their research in the field of

molecular biology.

This is your chance to gain international acclaim and recognition

for yourself and your faculty, and to turn your scientific ideas

into reality.

• If you were awarded your Ph.D. in molecular biology* during

2007, describe your work in a 1000-word essay.

• Submit your essay for the 2008 GE & Science Prize for Young

Life Scientists.

• A panel of distinguished scientists will select one Grand Prize

winner and four regional winners.

• Submission deadline August 1.

Few awards in science are more rewarding than the Science/AAAS

and GE Healthcare-sponsored prize – just ask the Grand Prize

winner for 2007. In addition to having his essay published in

Science, Matthew Stremlau received $25,000, was flown to the

awards ceremony in Stockholm, Sweden, and got to pick the

brains of Nobel laureates Mario R. Capecchi, Sir Martin J. Evans

and Oliver Smithies.**

GE & Science Prize for Young Life Scientists:

Ph.D. Research Re-imagined.

For more information, go to

www.gelifesciences.com/science

Get published in Science, meetthe Nobel laureates, win a tripto Stockholm and $25,000.

* For the purpose of this prize, molecular biology is defined as “that partof biology which attempts to interpret biological events in terms of thephysico-chemical properties of molecules in a cell” (McGraw-Hill Dictionaryof Scientific and Technical Terms, 4th Edition).

**Nobel Prize 2007 winners in Physiology or Medicine for their discoveriesof principles for introducing specific gene modifications in mice by theuse of embryonic stem cells.

Grand Prize winner Matthew Stremlau presents his

research to a captivated audience

Prize winners with Nobel laureates Mario R. Capecchi,

Sir Martin J. Evans and Oliver Smithies.

Submission deadline

August 1

Page 14: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

Proteinmodification by ubiquitin or the ubiquitin-like proteinshas been implicated in a variety of cellular processes includingproteosomal degradation, cell signaling, gene transcription, DNArepair, and protein localization. The nature, location, and extentof these posttranslational modifications appear to influence theircellular fate. Recent evidence that small molecule inhibitors ofthe proteasome can be used in the treatment of human cancershas launched intensive efforts to discover new drugs that willselectively inhibit the activities of disease-specific components ofthe ubiquitin pathway. It has also stimulated the development ofnew tools to help to better understand the complexmechanismssurrounding ubiquitin.

Webinar sponsored by Invitrogen

Youwill meetwith a panel of experts to:

• Learnmore about the role of ubiquitin in cellular processes.

• Find out the latest about efforts to find new drugs that targetthe ubiquitin pathway.

• Hear about the successful application of proteinmicroarraytechnology for discovering new substrates for ubiquitin ligases.

• Have your questions answered by the experts live and inreal time.

www.sciencemag.org/webinar

For more informationand complimentaryregistration visit:

Register now!

TheUbiquitin-Proteasome Pathway:Targets for Disease Treatment andNewTools for Discovery

Brought to you by the ScienceBusiness Office

Join our panel of experts in a LIVEonline seminar onMay 1, 200812 noon EDT, 9 amPDT, 5 pmGMT

TheUbiquitin-Proteasome

PathwayWEBINAR

Participating Experts:

Alfred Goldberg, Ph.D.Department of Cell BiologyHarvardMedical SchoolBoston,MA

Paul Andrews, Ph.D.AmgenCambridge,MA

Barry Schweitzer, Ph.D.InvitrogenCarlsbad, CA

Page 15: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

PNASProceedings of the National Academy of Sciences of the United States of America

www.pnas.org

Cozzarelli Prize

CLASS I: PHYSICAL ANDMATHEMATICAL SCIENCES

Understanding the nanoparticle–protein

corona using methods to quantify

exchange rates and a�nities of proteins

for nanoparticlesTommy Cedervall, Iseult Lynch, Stina Lindman,Tord Berggård, Eva�ulin, Hanna Nilsson,Kenneth A. Dawson, and Sara Linse

(2007) PNAS 104:2050–2055

CLASS II: BIOLOGICAL SCIENCES

Müller cells are living optical �bers in the

vertebrate retinaKristian Franze, Jens Grosche, Serguei N.Skatchkov, Stefan Schinkinger, Christian Foja,Detlev Schild, Ortrud Uckermann, Kort Travis,Andreas Reichenbach, and Jochen Guck

(2007) PNAS 104:8287–8292

CLASS III: ENGINEERING AND APPLIED SCIENCES

Faceting ionic shells into icosahedra via

electrostaticsGraziano Vernizzi and Monica Olvera de la Cruz

(2007) PNAS 104:18382–18386

CLASS IV: BIOMEDICAL SCIENCES

Targeted delivery of proteins across the

blood–brain barrierBrian J. Spencer and Inder M. Verma

(2007) PNAS 104:7594–7599

CLASS V: BEHAVIORAL AND SOCIAL SCIENCES

Savanna chimpanzees use tools to harvest

the underground storage organs of plantsR. Adriana Hernandez-Aguilar, Jim Moore,and Travis Rayne Pickering

(2007) PNAS 104:19210–19213

CLASS VI: APPLIED BIOLOGICAL, AGRICULTURAL,

AND ENVIRONMENTAL SCIENCES

Incorporating plant functional diversity

e�ects in ecosystem service assessmentsSandra Díaz, Sandra Lavorel, Francescode Bello, Fabien Quétier, Karl Grigulis,and T. Matthew Robson

(2007) PNAS 104:20684–20689

2007 COZZARELLI PRIZE RECIPIENTS

The Proceedings of the National Academy of Sciences (PNAS) has selected sixoutstanding articles for the 2007 Cozzarelli Prize, in recognition of their scienti�c

excellence and originality. Winners were selected from the 3,600 research articlespublished in PNAS in 2007 and represent exceptional contributions to the six broadlyde�ned classes under which the National Academy of Sciences is organized.

Page 16: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

From the publishers of Science, Science Signaling, formerly known as

Science’s STKE, will add original research starting September 2008.

Each week the journal will publish leading-edge findings in addition

to the current features, including Perspectives, Reviews, Protocols,

Meeting Reports, Book Reviews, Teaching Resources, and the

Database of Cell Signaling.

Science Signaling showcases high-impact research in cellular

regulation in such fields as molecular biology, development,

immunology, neuroscience, microbiology, physiology and medicine,

pharmacology, biochemistry, cell biology, bioinformatics, and systems

biology. Submit your research that provides new concepts and new

understanding of biological signal transduction for consideration.

Subscribing to Science Signaling ensures that you and your lab have

the latest cell signaling resources. From basic science to design of

therapeutics, from molecules to networks and systems design, read

the best source – Science Signaling.

Submit your paper to:stke.sciencemag.org/about/help/research.dtl

ISSN: 1937-9145

Page 17: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

www.sciencemag.org/products SCIENCE VOL 320 18 APRIL 2008 389

AAAS/Science Business Office

New Products Focus: Cell/Tissue Culture

LIFE SCIENCE TECHNOLOGIES

Vibration-Isolation WorkstationsSturdy, versatile, and ergonomic, this line of vibration-isolation workstationsfeatures automatic leveling and low natural frequencies for excellent vertical andhorizontal isolation efficiencies. The workstations can be configured for a widevariety of applications in which external vibrations could adversely affect theoperation of sensitive equipment. The Standard High-Performance Workstation isthe choice for most applications. A Heavy/Moving-Load Workstation is availableto isolate loads of up to 2,800 pounds, including equipment with high centersof gravity. The Personal Workstation is an economical solution for small labs andother tight spaces. A Variable-Height Workstation allows the user to raise or lowerthe tabletop to accommodate the height of the user. The Cleanroom Workstationis designed for use in Class 1 and Class 10 cleanrooms. The MK26 Workstationmakes use of the Minus K stiff spring and negative-stiffness mechanism toachieve low net vertical stiffness, ultralow natural frequencies, and high internalstructural frequencies without affecting the static load supporting capability.

Supercontinuum Confocal MicroscopeThe Leica TCS SP5 X confocal microscope with supercontinuum laserprovides freedom and flexibility that were unattainable until nowin fluorescence microscopy. The supercontinuum laser allows theresearcher to choose any excitation line within the continuous rangeof 470 nm to 670 nm. The Leica TCS SP5 X can precisely adapt toany fluorescent dye. Adjustment of the excitation line to the samplein 1 nm increments reduces cross-excitation and minimizes sampledamage. The system allows multicolor excitation with up to eightexcitation lines simultaneously. The patented Spectral DetectionTechnology provides the flexibility to choose any detection areain up to five spectral confocal photomultiplier tube channels. Thesystem includes the acousto-optical beamsplitter, a Leica inventionthat provides high sensitivity and better transmission than any otherdichroic mirror.LeicaFor information 800-248-0123www.leica-microsystems.com

Piezo Stage SystemThe NZ500CE NanoScanZ Piezo 500 micron stage system is aninnovation in microscope automation designed for researchersusing deconvolution and three-dimensional imaging techniques.The NanoScanZ Piezo 500 offers five-nanometer repeatability andclosed-loop control with a subangstrom resolution piezo resistivesensor. The stage provides piezo speed with a sample holder arealarge enough for well plates in addition to standard slides and petridishes. The500-micronmovement enables theNZ500CE to focus overan entire well plate without having to adjust the microscope focusmechanism. The stage complements the speed of the newest digitalcameras and accomplishes in milliseconds what would take secondsfor conventional rotary focus drives. By moving the sample insteadof the objective, the NanoScanZ offers advantages over objective-based piezo systems, including faster movement and settling and theflexibility to create Z stacks with multiple objectives.Prior ScientificFor information 781-878-8442www.prior.com

PFilter Set Tailored to NanocrystalsInvitrogen’s Molecular Probes Qdot 625 nanocrystal fluorescentlabel is matched to work with the Brightline fluorescence filter setfrom Semrock. This new label-filter combination allows the ultimatein brilliant imaging or quantitation by combining Invitrogen’s stableand technically advanced Qdot nanocrystal fluorescence labelingtechnology with Semrock’s matched “no burn out” high performance

Newly offered instrumentation, apparatus, and laboratory materials ofinterest to researchers in all disciplines in academic, industrial, and gov-ernmental organizations are featured in this space. Emphasis is given topurpose, chief characteristics, and availabilty of products and materials.Endorsement by Science or AAAS of any products or materials mentionedis not implied. Additional information may be obtained from the manufac-turer or supplier.

Electronically submit your new product description or product literatureinformation! Go to www.sciencemag.org/products/newproducts.dtl formore information.

fluorescence filters. Qdot fluorescence labeling products’ imagingperformance comes from their robust nanocrystal structure combinedwith a customizable surface. As one example of their wide range ofapplications, the Qdot 625 streptavidin conjugate fills an importantspectral gap for dense multicolor imaging experiments and exhibitsgreater brightness than the longer-wavelength Qdot nanocrystals.Coupled with Semrock’s high-brightness matched fluorescence filterset, these products are suitable for demanding experiments suchas live-cell, single-molecule dynamic imaging. The Qdot labels arenot only bright, but usable in applications that require long-termphotostability.Semrock and InvitrogenFor information 585-594-7003www.semrock.com

Multispectral Imaging System FXThe Kodak In-Vivo Multispectral Imaging System FX is a new digitalsystem for fluorescent molecular imaging that enables life scienceresearchers to study molecular abnormalities that are the origin ofdisease at a very early stage. The system is designed to preciselylocate and monitor changes in the molecular activity of specificcells or organs long before morphological changes can be detected,expediting the development of effective therapeutics for diseasetreatment.Thesystem’suniquemultispectral tuningof excitation lightgreatly enhances image sensitivity, allowing for the identification andseparation of multiple optical biological marker signals in subjectssuchassmallanimals,while removingbackgroundnoise.Thesystem’ssophisticated software automatically generates and analyzes a seriesof images taken at different wavelengths with spatially coregisteredX-ray and white light images for improved localization of biomarkersin vivo.Thesystem’smultimodal capabilities includebioluminescenceand radioisotopic signal detection.Carestream HealthFor information 877-747-4357www.carestreamhealth.com

Kinetic SystemsFor information 617-522-8700www.kineticsystems.com

New Products Focus: Microscopy

Page 18: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal
Page 19: Did you know that elephants are one of the few …...Finnzymes’ novel PCR enzyme outperforms every Taq-based hot start polymerase on the market. Phire DNA Polymerase is an ideal

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