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One-stop HybridomaDevelopment Services
DEVELOPMENT OF
MONOCLONAL ANTIBODIES
WHAT WE DO:
© 2007 - 2018 Creative-Biolabs All Rights Reserved Email: [email protected] Tel: 1-631-381-2994
Surface Display Services
Native™ Antibody Discovery by B Cell
scFv/Fab/sdAbgenes
Premadelibrary
PCR
Display
Repeatscreening
Screening
Cell culture
Myeloma cells
Hybridoma
Isolated B cells
Surfacedisplaylibrary
Immunized host animals (e.g. mouse )
Humanized mouse
Target IgG genes
IsolatedB cell
Spleen B cell
Antibody SourceCreative Biolabs can offer a series of good source for specific antibody discovery and development, which including but not limited to a wide range of host animal species, transgenic rodent, and high-quality premade libraries. In terms of our advanced antibody discovery techniques, we can discover mAbs from a broad range of species and also the Native™ human mAbs with naïve paired heavy-and light-chain.
HybridomaThe B cell that produces antibodies are harvested from the mouse and are in turn fused with immortal B cell cancer cells, a myeloma, to produce a hybrid cell line called a hybrid-oma.
Surface DisplayHuman VH and VL antibody genes are isolated from naive or infected individuals and randomly cloned for surface display. Currently developed surface display systems are mainly based on phage, bacteria, yeast, ribosome, and mammalian cell.
B-cell SortingAntigen-specific B cells can be captured by flow cytometry technology. Antibody light and heavy chain variable gene sequences are amplified from single B cells by RT-PCR. Then the gene transcripts are sequenced, identified and analyzed. To determine the properties of antibodies, the Ig gene are cloned into expression vectors for recom-binant production.
Selection
Cloning
mAb, Human mAb, Humanized mAb
mAb, Human mAb with Naïve-paired H- and L-chain
mAb, Humanized mAb
Infected patients
Hybridoma(Rodent & Rabbit)
Transgene
B-cell Sorting
Surface Display
Phage Display ServiceBacterial Display ServiceRibosome Display ServiceYeast Display ServiceMammalian Cell Display Service
Native™ Human AntibodyNative™ Monkey AntibodyNative™ Mouse AntibodyNative™ Camel Antibody......
Antigen Preparation Immunization Hybridoma Generation Hybridoma Screening
Specific B cell capture
Screening
RT-PCRCloning
Antibody genes cloning
Antibody expression
Ag-specific B cell
Antibody Production & PurificationWe can generate high purity antibody products as scFv, Fab, IgG or other fusion protein format. A broad rang of expression system is also avaialbe for recombinant production, which including but not limited to bacterial system, mammalian system, yeast system, and insect system.
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B cell
