1
Developing and Testing Guava- and Jackfruit-nut- bars to Bridge Seasonal Food Gaps in East Africa Sirui Xing 1 , Gudrun B. Keding 2 and Elke Pawelzik 3 1 University of Goettingen, Department of crop science, Division of quality of plant products 2 University of Goettingen, Department of crop science, Germany 3 University of Goettingen, Department of crop science , Division of quality of plant products, Germany Introduction Guava (Psidium guajava) and jackfruit (Artocarpus heterophyllus) are naturalized fruits in East Africa with high contents of nutrients essential for human bodies. However, through improper post-harvest treatments and only seasonal availability many nutrients or even whole surplus fruits are lost while at the same time fruit consumption in East Africa is far below the recommended amounts. In the framework of a joint project on “Fruits and vegetables for all seasons” with partners in East Africa and Germany, this study aimed to explore the development of nutritional fruit-nut-bars with a long shelf-life. The effects of common processing methods (cooking and oven drying) and varying ingredients (mango, lemon juice, desiccated coconut, peanut and cashew nut) on nutrient composition in fruit-nut-bars were measured. Methodology Ascorbic acid: titration by using 2,6-Dichlorophenolindophenol (DIP) dye (Puwastien et al., 2011). Total phenolic content: by using Folin & Ciocalteu’s phenol reagent (Folin-C reagent) and photometrical determination (Singleton et al., 1965). Titratable acidity: titration by using 0.1N NaOH solution (LMBG, 1983). Water content: by drying for 19 hours at 60 followed four hours at 105 (Slatyer et al., 1968). Mineral nutrients: sample preparation by microwave extraction with the microwave, measurement of minerals by ICP-OES (Wheal et al., 2011). Conclusions Cooking and drying procedures decrease, as expected, nutrient contents, yet, to varying extent. While the consumption of fresh fruits is always the better choice, the fruit-nut-bars provide a good option to process surplus fruits and bridge seasonal gaps. Results Bars with guava and lemon juice contained the highest content of ascorbic acid (81.19 ± 0.37 mg/100g FM) (Figure 1). Water content of final products was <10 %, which indicated a longer shelf life. G1=Guava-Cashew-Lemon, G2=Guava-Cashew, bc=before cooking, ac=after cooking Based on the results of this study, the ascorbic acid content has decreased by 54% during the production of fruit bars, so that the final guava products still contain about 27 mg ascorbic acid in 100g DM. According to the USA Institute of Medicine (1998), for teenagers at the age of 9-13 years old, the recommended dietary allowances (RDAs) of ascorbic acid is 45 mg per day. This means that the consumption of the fruit bar could still be beneficial to children as well as other population groups in East Africa. Fruit bars with lemon juice had higher acidity (Figure 2). For phenolic content, most samples of jackfruit contained more than guava products (Figure 3). Figure 1: Ascorbic acid content of guava samples (n=3, mean ± SD) Results According to Institute of Medicine (2011), for teenagers at the age of 9-13 years old, the dietary reference intakes (DRIs) of Fe and Zn is 8 mg per day. In general, bars in this study can provide sufficient essential nutrients for teenagers in East Africa (Table 1). Table 1: Mineral content of guava and jackfruit samples Figure 3: Total phenolic content of guava and jackfruit samples after drying (n=2, mean ± SD) References: Institute of Medicine. 1998. “Dietary Reference Intakes (DRIs): Vitamins.” Washington (DC): National Academy Press, 1997. www.nap.edu. Institute of Medicine. 2011. “Dietary Reference Intakes ( DRIs ): Elements Food and Nutrition Board.National Academies. Puwastien, P., Siong, T. E., G. Kantasubrata, J., Craven, and K. Feliciano, R. R., Judprasong. 2011. “Manual of Food Analysis.” Asean. Singleton, V L, and J A Jr. Rossi. 1965. “Colorimetry to Total Phenolics with Phosphomolybdic Acid Reagents.” American journal of enology and viniculture. Rutter, A. J., and R. O. Slatyer. 1968. “Plant-Water Relationships.” The Journal of Applied Ecology. Wheal, Matthew S., Teresa O. Fowles, and Lyndon T. Palmer. 2011. “A Cost-Effective Acid Digestion Method Using Closed Polypropylene Tubes for Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) Analysis of Plant Essential Elements.” Analytical Methods. Jackfruit-based fruit-nut-bars after 6-hour-drying © Sirui Xing Guava-based fruit-nut-bars after 19-hour drying © Sirui Xing Guava-based fruit-nut-bars after 6-hour-drying © Sirui Xing Guava-based fruit-nut-powder © Sirui Xing Jackfruit-based fruit-nut-bars after 19-hour-drying © Sirui Xing Mineral element Content (mg/g DM) DRI values are suitable for 9-13 years old teenager (mg/day) Potassium (K) 11.96±1.30 4500 Phosphorous (P) 4.31±0.16 1250 Magnesium (Mg) 1.76±0.13 1.6-1.9 Copper (Cu) 0.07±0.01 0.7 Iron (Fe) 0.06±0.02 8 Zinc (Zn) 0.05±0.01 8 Manganese (Mn) 0.02±0.00 1.6-1.9 Figure 2: Titratable acidity of guava and jackfruit samples after drying (n=2, mean ± SD) . 0 20 40 60 80 100 G1bc G1ac G2bc G2ac Content (mg/100g FM) Samples 0 0.2 0.4 0.6 0.8 1 G1ad G2ad G3ad G4ad J1ad J2ad J3ad J4ad TA (%) Samples G1=Guava-Cashew-Lemon, G2=Guava-Cashew, G3=Guava-Peanut-Lemon, G4=Guava-Peanut, J1=Jackfruit-Cashew-Lemon, J2=Jackfruit-Cashew, J3=Jackfruit-Peanut-Lemon, J4= Jackfruit-Peanut, ad=after drying 0 200 400 600 800 1000 1200 G1ad G2ad G3ad G4ad J1ad J2ad J3ad J4ad Concentration (μg GAE/100g FM) Samples G1=Guava-Cashew-Lemon, G2=Guava-Cashew, G3=Guava-Peanut-Lemon, G4=Guava-Peanut, J1=Jackfruit-Cashew-Lemon, J2=Jackfruit-Cashew, J3=Jackfruit-Peanut-Lemon, J4= Jackfruit-Peanut, ad=after drying DRI: dietary reference intakes Source: Government of Canada

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Page 1: Developing and Testing Guava- and Jackfruit-nut- bars to

Developing and Testing Guava- and Jackfruit-nut-bars to Bridge Seasonal Food Gaps in East Africa

Sirui Xing1, Gudrun B. Keding2 and Elke Pawelzik3

1University of Goettingen, Department of crop science, Division of quality of plant products 2 University of Goettingen, Department of crop science, Germany 3 University of Goettingen, Department of crop science , Division of quality of plant products, Germany

IntroductionGuava (Psidium guajava) and jackfruit (Artocarpus heterophyllus) arenaturalized fruits in East Africa with high contents of nutrients essential forhuman bodies. However, through improper post-harvest treatments and onlyseasonal availability many nutrients or even whole surplus fruits are lost whileat the same time fruit consumption in East Africa is far below therecommended amounts.

In the framework of a joint project on “Fruits and vegetables for all seasons”with partners in East Africa and Germany, this study aimed to explore thedevelopment of nutritional fruit-nut-bars with a long shelf-life. The effects ofcommon processing methods (cooking and oven drying) and varyingingredients (mango, lemon juice, desiccated coconut, peanut and cashew nut)on nutrient composition in fruit-nut-bars were measured.

Methodology▪ Ascorbic acid: titration by using 2,6-Dichlorophenolindophenol (DIP) dye

(Puwastien et al., 2011).

▪ Total phenolic content: by using Folin & Ciocalteu’s phenol reagent (Folin-Creagent) and photometrical determination (Singleton et al., 1965).

▪ Titratable acidity: titration by using 0.1N NaOH solution (LMBG, 1983).

▪ Water content: by drying for 19 hours at 60℃ followed four hours at 105℃(Slatyer et al., 1968).

▪ Mineral nutrients: sample preparation by microwave extraction with the microwave, measurement of minerals by ICP-OES (Wheal et al., 2011).

ConclusionsCooking and drying procedures decrease, as expected, nutrient contents, yet, tovarying extent. While the consumption of fresh fruits is always the better choice,the fruit-nut-bars provide a good option to process surplus fruits and bridgeseasonal gaps.

Results▪ Bars with guava and lemon juice contained the highest content of ascorbic

acid (81.19 ± 0.37 mg/100g FM) (Figure 1).

▪ Water content of final products was <10 %, which indicated a longer shelflife.

G1=Guava-Cashew-Lemon, G2=Guava-Cashew, bc=before cooking, ac=after cooking

▪ Based on the results of this study, the ascorbic acid content hasdecreased by 54% during the production of fruit bars, so that the finalguava products still contain about 27 mg ascorbic acid in 100g DM.According to the USA Institute of Medicine (1998), for teenagers at theage of 9-13 years old, the recommended dietary allowances (RDAs) ofascorbic acid is 45 mg per day. This means that the consumption of thefruit bar could still be beneficial to children as well as other populationgroups in East Africa.

▪ Fruit bars with lemon juice had higher acidity (Figure 2).

▪ For phenolic content, most samples of jackfruit contained more thanguava products (Figure 3).

Figure 1: Ascorbic acid content of guava samples (n=3, mean ± SD)

ResultsAccording to Institute of Medicine (2011), for teenagers at the age of 9-13years old, the dietary reference intakes (DRIs) of Fe and Zn is 8 mg per day. Ingeneral, bars in this study can provide sufficient essential nutrients forteenagers in East Africa (Table 1).

Table 1: Mineral content of guava and jackfruit samples

Figure 3: Total phenolic content of guava and jackfruit samples after drying(n=2, mean ± SD)

References:Institute of Medicine. 1998. “Dietary Reference Intakes (DRIs): Vitamins.” Washington (DC): National AcademyPress, 1997. www.nap.edu.Institute of Medicine. 2011. “Dietary Reference Intakes ( DRIs ): Elements Food and Nutrition Board.” NationalAcademies.Puwastien, P., Siong, T. E., G. Kantasubrata, J., Craven, and K. Feliciano, R. R., Judprasong. 2011. “Manual of FoodAnalysis.” Asean.Singleton, V L, and J A Jr. Rossi. 1965. “Colorimetry to Total Phenolics with Phosphomolybdic Acid Reagents.”American journal of enology and viniculture.Rutter, A. J., and R. O. Slatyer. 1968. “Plant-Water Relationships.” The Journal of Applied Ecology.Wheal, Matthew S., Teresa O. Fowles, and Lyndon T. Palmer. 2011. “A Cost-Effective Acid Digestion MethodUsing Closed Polypropylene Tubes for Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES)Analysis of Plant Essential Elements.” Analytical Methods.

Jackfruit-based fruit-nut-bars after 6-hour-drying © Sirui Xing

Guava-based fruit-nut-bars after 19-hour drying© Sirui Xing

Guava-based fruit-nut-bars after 6-hour-drying© Sirui Xing

Guava-based fruit-nut-powder© Sirui Xing

Jackfruit-based fruit-nut-bars after 19-hour-drying© Sirui Xing

Mineral element

Content (mg/g DM)

DRI values are suitable for 9-13 years old teenager (mg/day)

Potassium (K) 11.96±1.30 4500

Phosphorous (P) 4.31±0.16 1250

Magnesium (Mg) 1.76±0.13 1.6-1.9

Copper (Cu) 0.07±0.01 0.7

Iron (Fe) 0.06±0.02 8

Zinc (Zn) 0.05±0.01 8

Manganese (Mn) 0.02±0.00 1.6-1.9

Figure 2: Titratable acidity of guava and jackfruit samples after drying (n=2, mean ± SD) .

0

20

40

60

80

100

G1bc G1ac G2bc G2acCo

nte

nt

(mg

/10

0g

FM)

Samples

0

0.2

0.4

0.6

0.8

1

G1ad G2ad G3ad G4ad J1ad J2ad J3ad J4ad

TA (

%)

Samples

G1=Guava-Cashew-Lemon, G2=Guava-Cashew, G3=Guava-Peanut-Lemon, G4=Guava-Peanut,

J1=Jackfruit-Cashew-Lemon, J2=Jackfruit-Cashew, J3=Jackfruit-Peanut-Lemon,

J4= Jackfruit-Peanut, ad=after drying

0

200

400

600

800

1000

1200

G1ad G2ad G3ad G4ad J1ad J2ad J3ad J4ad

Co

nce

ntr

atio

n

(µg

GA

E/1

00

g FM

)

Samples

G1=Guava-Cashew-Lemon, G2=Guava-Cashew, G3=Guava-Peanut-Lemon, G4=Guava-Peanut,

J1=Jackfruit-Cashew-Lemon, J2=Jackfruit-Cashew, J3=Jackfruit-Peanut-Lemon,

J4= Jackfruit-Peanut, ad=after drying

DRI: dietary reference intakes Source: Government of Canada