Determination of ochratoxin A in wine by means of immunoaffinity column clean-up and high-performance

liquid chromatography

N

COOH

H

O

O

OH

Cl

O

Ochratoxin A (OTA)

ToxicityOTA is hepatotoxic, nephrotoxic, teratogenic and carcinogenic to animalsOTA was classified by the International Agency for Research on Cancer (IARC) as a possible human carcinogen (group 2B)

Regulatory limits set by the European UnionCOMMISSION REGULATION (EC) No 123/20052,0 ppb for wine (red, rose and white)

The clean-up procedure for ochratoxin A involves generally IAC, SPEImmunoaffinity chromatography (IAC) clean-up is based on the binding interaction of ochratoxin A-specific antibodies and has been set as European Standard (SR EN 14133) for ochratoxin A analysis in wine and beer

Method

Immunoaffinity column design containing solid extraction phase made from

antibody-modified support beads binding ochratoxin A

Chromatographic determination

Sample preparation and clean-up

Sample preparation and immunoaffinity clean-up

Chromatogram of OTA contaminated red wine

Chromatographic conditions: OTA was quantified by reverse phase high–performance liquid chromatography (HPLC) with fluorescence detection (excitation wavelength 330nm, emission wavelength 450nm).mobile phase consisted of a mixture of acetonitrile–water–acetic acid (99:99:2)analytical column were reverse phase, C-18 with 5 μm particlesflow-rate 1 mL/minInjection 20 yL

HPLC determination of ochratoxin A