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Department of Genetic EngineeringSRM University
Lesson Plan
3rd Semester - B.Tech Genetic Engineering2011-12
BT0201 Enzyme technology BT0203 Genetics and Cytogenetics BT0205 ImmunologyBT0207 MicrobiologyCH0215 Mechanical operations and Heat transfer LE0201 German Language Phase I LE0203 Japanese Language Phase I LE0205 French Language Phase I BT0217 Computer Skills
BT0215 Immunology Laboratory BT0211 Microbiology Laboratory
SRM University, Kattankulathur, Chennai-603203Ph: 044-27417817/57 Email: [email protected]
www.srmuniv.ac.in
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Branch: B.Tech Genetic Engineering Semester:III
Academic Year:2011-12
Faculty: P. Rathinasabapathi Email: [email protected]
Lecture
NoDescription
1 Introduction to Enzymes2 Classification of enzymes3 specificity of enzyme action4 monomeric and oligomeric enzymes5 Factors modifying enzyme activity6 Application of enzymes7 Revision 8 Structural Components of Enzymes , apoenzymes, prosthetic group9 Cofactors and their involvement in enzyme activity
10,11 Mechanisms of reactions catalysed by enzymes12 Metal activated enzymes - metalloenzymes13 Involvement of coenzyme in enzyme catalyzed reactions14 Revision
15,16 Kinetics of single substrate reactions.17,18 Kinetics of multi-substrate reactions19-21 Problem based on single and multiple substrate reaction
22 Temperature and pH effects on enzyme activity23 Allosteric enzymes: The Monad - Changeux - Wyman model (MCW)24 Koshland - Nemethy - Filmer (KNF) model25 Methods of immobilization of enzymes
26-28 Kinetics of immobilized enzymes: Effects of external mass transfer and intra29 Revision30 Methods of production of enzymes
31,32 Extraction of Enzymes : soluble enzymes - membrane bound enzymes33-35 Purification of enzyme36,37 Determination of molecular weight of enzymes
38 Revision39 Manometry 40 Spectrophotometry - Spectrofluorimetry41 Enthalpimetry -42 Electrochemical methods43 Radio chemical methods44 Automation in enzymatic analysis45 Revision
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Code Course Name L T P CBT0201 Enzyme Technology 3 0 0 3
Code Course Name L T P CBT0203 Genetics and Cytogenetics 3 0 0 3
Branch : B. Tech Genetic Engineering Semester: IIIAcademic Year:2011-12
Faculty: Mr. D. Rex Arunraj, Email: [email protected]
Lecture No.
Description
1 Introduction to Genetics and cytogenetics
2 History of Genetics, Mendel’s Experiments
3 Principles of Segregation – Monohybrid Cross
4 Independent Assortment-I
5 Independent Assortment-II
6 Gene Interaction I
7 Gene Interaction II
8 Gene Interaction III
9 Multiple Alleles
10 Review & Test
11 Chromosome Structure and Organization – Prokaryote
12 Chromosome Structure and Organization – Eukaryote I
13 Chromosome Structure and Organization – Eukaryote II
14 Giant chromosomes – polytene
15 Giant chromosomes – lampbrush
16 sex determination
17 Sex linkage I
18 Sex linkage II
19 Review & Test
20 Linkage
21 crossing over
22 Cytological basis – Linkage and crossing over I
23 Cytological basis – Linkage and crossing over II
24 Chromosome Mapping – Two factor Cross
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25 Three Point Cross
26 Interference and Somatic cell hybridization
27 Three Point Cross Problem solving
28 Review & Test
29 Duplication – Inversion
30 Translocation – Effects
31 Karyotyping – Idiogram
32 Chromosomal aberrations in Humans
33 Classification of Mutation
34 Ploidy and Ploidy variation
35 Extra chromosomal Inheritance
36 Cytogenetic abnormalities in Humans
37 Review & Test
38 Transformation and Mapping
39 Transduction and Mapping
40 Conjugation and Mapping
41 Mapping in Merozygotes
42 Plasmids and Episomes
43 Review & Test
Code Course Title L T P C
BT0205 Immunology 3 0 0 3
Branch: B.Tech Genetic Engineering Semester: III
Academic Year:2011-12
Faculty: Dr.M. Ramya Email: [email protected]
Lecture No. Description1 -4 Introduction – overview of the immune system
Lymphatic system, Lymphoid organs, Cells of the immune system and their functions,
5-6 Immune system-Innate and Acquired immunity7-8 Innate immunity9-10 Adaptive immunity11 Comparative immunity: Anatomical and Physiological barriers; Innate
immune response and their recognition structures; Pathogen elimination; Plant Immune system
12-13 Immunogens and Antigens: Requirements for immunogenicity; major classes of antigens; antigen recognition by B and T lymphocytes
14-15 Immunoglobulin structure and function16 Antibody mediated effector functions
17-19 Antibody classes and biological activities20 Antigenic determinants on Ig21 B-cell receptor
22-24 Monoclonal antibodies25-27 Cytokine28-29 Complement system
30 Antigen- antibody interaction –antibody affinity and activity- precipitation reaction, agglutination reaction
31-32 Radio-immunoassay, ELISA, Western Blot, Immunoprecipitation33-34 Immunofluoresence, immuno electron microscopy, flow cytometry35-37 MHC, antigen processing and presentations
38 T-cell receptors39-40 T-cell maturation, activation and differentiation; Cell mediated effector
responses41 B-cell generation, activation and differentiation42 Leukocyte migration and inflammation43 Hypersensitive reactions44 Immune responses to infectious diseases45 Vaccines
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Code Course Title L T P CBT0207 Microbiololgy 3 0 0 3
Branch : B. Tech Genetic Engineering Semester: IIIAcademic Year:2011-12
Faculty: Mr. B.S. Darshan Email: [email protected]
Lecture No.
Description
1. Characterization of microorganisms 2.
Classification and Identification of microorganisms3.
Microscopic examination of Micro organisms4. Morphology and fine structure of bacteria
External structure5.
Internal structure6. Cultivation of bacteria7. Reproduction 8.
Growth9. Pure cultures and cultural characteristics.10. Enzymes - Introduction, classification of enzymes, factors affecting enzyme
activity.11. Enzymes and their regulation12. Microbial metabolism energy production Electron Transport chain,
Glycolysis, TCA.13. Microbial metabolism energy production
HMP, Entner Duodorf pathway14. Utilization of energy & biosynthesis15.
Utilization of energy & biosynthesis16.
Bacterial genetics-Transformation17.
Conjugation18.
Transduction19.
Fungi – importance, characteristics, morphology20.
Reproduction, physiology cultivation of fungi
21. Classification of fungi and molds 22. Repair association with other organisms
23. Algae – importance of algae – characteristics of algae24. Classification protozoa: Ecology, importance, morphology, reproduction
and classification of protozoa
25.Control of micro organisms- Introduction
26.Control of micro organisms by physical methods
27.Control of micro organisms by chemical methods
28. Bacteriophages- General characteristics29. Morphology and structure, Classification of Bacteriophages30. Nomenclatureof Bacteriophages 31. Bacteriophages of E.coli32. Bacteriophages Replication33. Viruses of plants 34. Viruses of animals35.
Structure of viruses and its replication.36.
Classification- isolation and identification-fatal diseases associated with viruses in animals-viroids
37. Microbiology of soil38.
Bio-geo chemical cycles39. Aquatic microbiology40.
Aquatic microbiology41.
Microbiology of domestic water42.
Microbiology of waste water43. Microbiology of waste water44. Microbiology of food45.
Microbiology of Industrial microbiology
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Code Course Title L T P CCH0215 Mechanical operations and Heat transfer 3 0 0 3
Branch: B.Tech Genetic Engineering Semester: III
Academic Year:2011-12
Faculty: Ms. E. Kavitha Email:[email protected]
Lecture No.
Description
1 Introduction to various modes of heat transfer2 Fourier’s law of heat conduction3 Effect of temperature on thermal conductivity4 Steady-state conduction5 Compound resistances in series6 Compound resistances in series7 Heat flow through a cylinder8 Heat flow through a cylinder
9 Critical radius of insulation in pipes10 Introduction to convection11 Heat flux, average temperature of fluid stream12 Overall heat transfer coefficient13 LMTD, individual heat transfer coefficients14 Relationship between individual and overall heat transfer coefficients15 Application of dimensional analysis for convection16 Heat transfer coefficient calculation for natural and forced convection17 Heat transfer from condensing vapours, dropwise and film-type condensation18 Heat transfer coefficients calculation for film-type condensation19 Introduction to Heat Exchanger20 Counter current and parallel-current flows21 Enthalpy balances in: heat exchangers,total condensers.22 Double pipe Heat exchanger, Single-pass 1-1 Heat exchanger23 1-2 parallel-counterflow exchanger, 2-4 exchanger24 Heat-transfer coefficients in shell-and-tube exchanger25 correction of LMTD for crossflow26 Condensers: shell-and-tube condensers, kettle-type boilers27 Calculation of number of tubes in heat exchangers28 Introduction to filtration, cake filters29 Filter press30 Rotary drum vacuum filter31 Suspended batch centrifuge32 Filter media, filter aids33 Principle of cake filtration,pressure drop through filter cake, Compressible
and incompressible cake34 Filter medium resistance35 Constant pressure filtration, continuous filtration36 Constant rate filtration37 Principles of agitation38 dimensional analysis: Buckingham’s π theorem.39 Agitation equipment40 Flow patterns: prevention of Swirling and vortexing, draft tubes. 41 Power consumption & correlations42 Significance of dimensionless groups43 Effect of system geometry, calculation of power consumption in Newtonian
liquids44 Blending of miscible liquids, blending in process vessels45 Stratified blending in storage tank and equipments
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Code Course Title L T P CLE0201 GERMAN LANGUAGE PHASE I 2 0 0 2
Branch: B.Tech Genetic Engineering Semester:III
Academic Year:2011-12
Faculty: P. Tamilarasan Email: [email protected]
Lecture No. Description
1Introduction to Germany and German language and culture.Important cities and ports of Germany, Greetings
2Alphabets, Diphthongs Article (Definite and Indefinite), Numbers (till 20)
3Numbers continued (till million)Days of the week, Months, Seasons, Cardinal directions, Pg 24
4Pronouns, Verbs (sein, haben), Possessive articlesFamily, Occupations, Colors, Pg 30
5Regular verb conjugations, Negation (nicht, kein)Lesson 1 - vocabulary, reading and explanation
6 Revision, Accusative and negation, Directions7 Lesson 2 - vocabulary, reading and explanation8 Lesson 3 - vocabulary, reading and explanation9 Irregular and separable verbs conjugation, Pg 2110 Prepositions, W-Questions11 Lesson 4 - vocabulary, reading and explanation12 Grammar revision13 Question pattern and revision exercises14 Class test15 Oral examination
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Code Course Title L T P CLE0203 Japanese Language Phase I 2 0 0 2
Branch: B.Tech Genetic Engineering Semester:III
Academic Year:2011-12
Faculty: P.R. Rekhaa Email: [email protected]
DescriptionLecture no.
Introduction to Japanese/Japan – land, language and culture Phonetics and Greeting
1
Lesson 1 – Vocabulary, reading and explanation2
Drills, applied conversation and audio3Numbers 1 to 100Time – hours and minutes, kara and made
4
Lesson 2 - Vocabulary, reading and explanation 5
Drills, applied conversation and audio 6Numbers up to 99,999Grammar – Kore and kono
7
Grammar – Koko and kochira, arimasu and imasuAdjectives (intro to i ending and na ending adj) and colours
8
Lesson 3 – Reading, explanation and drills9
Dialogues, applied conversation and audio10
Vocabulary, particles – ni, ga and neIntroduction to Kanji (numbers) – p 46
11
Lesson 4 – Reading and explanationOther kanjis – p47
12
Drills, applied conversation and audio13
Quiz and revision14
Oral examination15
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Code Course Title L T P CLE0205 French Language Phase I 2 0 0 2
Branch: B.Tech Genetic Engineering Semester:III
Academic Year:2011-12
Faculty: J. Sabastian Email: [email protected]
Lecture No.
Description
1Introduction and usage of French languageL’alphabet (Pronunciation )Les voyelles (vowel sounds a,e,i,o,u,y,h)Salutations (greetings)Expressions
2Se présenterLes chiffres 1 à 50Interrogative (asking questions / name )Salutations avec beaucoup de vocabularies
3L’ aticle définiL’article indéfiniLes verbes auxilaires (Être / Avoir )1er groupe verbe –ERLeçon « Au secratariat »
4Les Pays / Les Nationalités / Les ProfessionsLes presentateurs « C’est / Ce sont »Leçon « En classe »Leçon « Dans le couloir »Pronom toniqueLes exercises
5Culture et civilisationCorrection of exercisesRevision
6Leçon “Autour de l’école” (translation)Vocabulaire (pronunciations)Les mots clesLa dictée
7Les pluriel du noms (plural of nouns)L’orientation de l’ espaceLes prépositions (in ,at ,to)Les exercices
8Les verbes en « ER » (conjugations)L’article contracté (du,de la des)L’article contracté (au, à la,aux)Les jours de la semaineLes exercices
9Les verbes irreguliers( Aller,Faire)Le Présentateu r « Il ya / y a-t-il »Les chiffres de 51 à l’infiniLa Communication
10Les exercises d’écoute (listening to CD and complete the exercise)La culture et civilizationLes exercices du texte
11Comment envoyer e-mailExplique la journéeLes VocabulairesLes mots clés
122nd groupe verbe – IR (Finir,Choisir)La negationLe pronom « on »Les exercices
13Pronom tonique apres la prépositionLes présentateurs « Voici / Voilà »Dire l’heureLes mois de laannée (months)Les exercises
14Dialogue (Comment proposer il sortie)Les activité, aller au cinémaDialogue conversation - aller au restaurantDialogue conversation – au téléphone / au portableCorrection - exercises
15La culture et civilisation (vin / fromage)Les exercises d’écoute (listening to CD and complete the exercise)Les exercises du texteL’ oral
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Code Course L T P CBT0217 Computer Skills 1 0 2 2
Branch: B. Tech Genetic EngineeringSemester: IIIYear: 2011-2012Faculty: Ms. D. Devahema Email: [email protected]
Lecture no. Description
1 Introduction to Database Management System,DDL,DML,Design
2 Simple queries
3 Builtin Functions
4 Group Functions
5 Multiple sub queries
6 SQL views and triggers
7 Bio Informatics DataBases
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Code Course L T P CBT0215 Immunology Laboratory 0 0 4 2
Branch: B. Tech Genetic Engineering Semester: IIIYear: 2011-2012Faculty: Dr. M.Ramya Email: [email protected]
Description
1 Blood Grouping2 Preparation of Widal Test (Qualitative & Quantitative )3 RPR Test4 Single Immuno Diffusion5 Double Immuno diffusion6 Rocket Immuno Electrophoresis7 Counter Current Immune Electrophoresis 8 Dot ELISA9 Differential Leukocyte counting10 Separation of serum proteins in SDS PAGE 11 Model Exam
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Code Course L T P CBT0211 Microbiology Laboratory 0 0 4 2
Branch: B. Tech Genetic Engineering Semester: IIIYear: 2011-2012Faculty: Mr. B.S. Darshan Email: [email protected]
ExperimentNo
Description
1 Sterilization Techniques2 Media preparation3 Microscopy and Micrometry4 Isolation, enumeration and purification of microbes from a given sample5 Staining Techniques (Simple, Gram staining, Spore staining)6 Biochemical Charaterization of Bacteria
- Oxidation/Fermentation Test- Catalase, Oxidase and Urease test- IMViC test- Hydrogen sulfide test and Nitrate Reduction test- Casein and starch hydrolysis
7 Antibiotic Assay – Antimicrobial Sensitivity Test (Disc diffusion method)8 Growth kinetics (Bacterial growth)9 Isolation of antibiotic producing bacteria10 Isolation And Characterization of plant microbes
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