Calc. Tiss. Res. 4 (Supplement), 83 (1970) Demonstration of Several Forms of Decalcification in Bone of the Teleost Fish, Anguilla anguilla L E. LoPEz Laboratory of General and Comparative Endocrinology and Laboratory of Comparative Endocrinology associated with C.N.R.S. Musdum National d'Histoire naturelle, Paris, France, Institute of Morphology, Medical School, Geneva, Switzerland The skeleton of the eel is made up of cellular bone. The cells present are those typical of bone of higher vertebrates: osteoblasts, osteocytes and osteoclasts. In the areas of bone resorption, osteoclasts in their erosion crypts (" Howship's lacunae ") are normally found. Some osteocytes~ mainly localized in the most peripheric layers of bone, are seen in enlarged lacunar cavities often surrounded by a demineralised zone. This area probably corresponds to the " perilaeunar bone resorption " or " osteolysis" (BAUD et al., 1961; BAW), 1968). In female eels, whose sexual evolution was induced by injections of carp pituitary extracts (FONTAINE et al., 1964), the degree of mineralization (amount of apatite per cubic em of bone) of the intercellular substance is strongly decreased without histological modifications of the organic matrix. This is probably due to a phenomenon of " halastasie" as defined by RU- TIS~AUSER et al., (1953) and it can be considered a response to an unbalance between calcified matrix and extracellular fluids, which could take place under the control of the system constituted by osteocytes and bordering cells (NICHOLS, 1963). This unstable part of the bone mineral could represent the amorphous fraction of the calcium phosphate of the apatite crystal. The deminera]isation in the bone of the Teleost fish can then be produced in three different ways: osteoelastie, osteolytic, halastasie. References 1. BAUD, C. A.: Clin. 0rthop. 56, 227--236 (1968). 2. BAUD, C. A., and S. WEBER-SLATI~INE: Bull. Micr. Apph 11, 73--76 (1961). 3. FONTAINE, 3I., E. BERTRAND, E, LO•EZ and O. CALLAMAND: C. ~:~. Acad. Sci. Paris 259, 2907--2910 (1964). 4. NIOtlOLS, G., Jr.: Mechanisms of hard tissue destruction, p. 557. R. F. SOGNNAES, ed. Washington: Amer. Ass. Advane. Sci. 1963. 5. RUTISHAUSE~, E., G. MAJNO and C. H. ROUILLEI~: Rev. Suisse Path. Gen. Baeterioh 167 118--124 (1953).

Demonstration of several forms of decalcification in bone of the Teleost fish,Anguilla anguilla L

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Calc. Tiss. Res. 4 (Supplement), 83 (1970)

Demonstration of Several Forms of Decalcification in Bone of the Teleost Fish, Anguilla anguilla L

E. LoPEz Laboratory of General and Comparative Endocrinology and

Laboratory of Comparative Endocrinology associated with C.N.R.S. Musdum National d'Histoire naturelle, Paris, France,

Institute of Morphology, Medical School, Geneva, Switzerland

The skeleton of the eel is made up of cellular bone. The cells present are those typical of bone of higher vertebrates: osteoblasts, osteocytes and osteoclasts.

In the areas of bone resorption, osteoclasts in their erosion crypts (" Howship's lacunae ") are normally found.

Some osteocytes~ mainly localized in the most peripheric layers of bone, are seen in enlarged lacunar cavities often surrounded by a demineralised zone. This area probably corresponds to the " peri laeunar bone resorption " or " os teolys is" (BAUD et al., 1961; BAW), 1968).

In female eels, whose sexual evolution was induced by injections of carp p i tu i ta ry extracts (FONTAINE et al., 1964), the degree of mineralization (amount of apat i te per cubic em of bone) of the intercellular substance is strongly decreased wi thout histological modifications of the organic matr ix. This is probably due to a phenomenon of " ha las tas i e" as defined by RU- TIS~AUSER et al., (1953) and it can be considered a response to an unbalance between calcified mat r ix and extracellular fluids, which could take place under the control of the system const i tuted by osteocytes and bordering cells (NICHOLS, 1963). This unstable par t of the bone mineral could represent the amorphous fract ion of the calcium phosphate of the apat i te crystal.

The deminera]isation in the bone of the Teleost fish can then be produced in three different ways: osteoelastie, osteolytic, halastasie.

References

1. BAUD, C. A.: Clin. 0rthop. 56, 227--236 (1968). 2. BAUD, C. A., and S. WEBER-SLATI~INE: Bull. Micr. Apph 11, 73--76 (1961). 3. FONTAINE, 3I., E. BERTRAND, E, LO•EZ and O. CALLAMAND: C. ~:~. Acad. Sci.

Paris 259, 2907--2910 (1964). 4. NIOtlOLS, G., Jr.: Mechanisms of hard tissue destruction, p. 557. R. F. SOGNNAES,

ed. Washington: Amer. Ass. Advane. Sci. 1963. 5. RUTISHAUSE~, E., G. MAJNO and C. H. ROUILLEI~: Rev. Suisse Path. Gen.

Baeterioh 167 118--124 (1953).