Dawn Coleman Resume - (2)

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  • Ms.DawnJ.ColemanDayPhone:3609518520 Email:dawn.jovan.coleman@gmail.comAvailability:JobType:Permanent,Temporary,InternshipsWorkSchedule:FullTime,PartTime

    Desiredlocations:UnitedStatesWAOlympiaUnitedStatesWATacoma

    WorkExperience:USArmyPublicHealthCommandPacificJBLMMCHBRWBox339500,MS115JointBaseLewisMcChordTacoma,WA98433UnitedStates10/2016PresentHoursperweek:25AmericanRedCrossVolunteerDuties,AccomplishmentsandRelatedSkills:

    PerformsdaytodayfunctionsoftemperaturesandmaintenanceofbiorepositorymachineryinordertomeetstrictaccreditationcriteriafortheAmericanAssociationforLaboratoryAccreditation(A2LA)andaccreditingbodies

    Assistswithmaintaininglaboratoryaccreditationbyadheringtoqualitycontrolandqualityassurancestandardsforlaboratoryfunctionsandoperations

    AssistsandstrictlyadherestolaboratorysafetyandhealthrequirementsnecessaryforBiosafetyLevel2laboratoryprocedures

    PerformsmolecularbasedtestingonenvironmentalorganismsincompliancewithStandardOperatingProcedures(SOPs)andLaboratoryInformationDatabase(LID)worksheetsfortechnicalandadministrativeprocessesIAWInternationalOrganizationforStandardization(ISO)/InternationalElectrotechnicalCommission(IEC)17025

    Performsandassistswithmaintainingasafeworkenvironmentthroughadherencetoregulatoryrequirementsinhandlinghazardousmaterials/wasteSafetyDataSheets(SDS)

    Operatesstateoftheartlaboratorytestingequipmenttoanalyzevectorbornediseases Conductsmolecularbasedtestingonenvironmentalorganismssuchasmosquitoes,ticks,fleas,

    androdentsusingconventionalandrealtimepolymerasechainreaction(PCR)andgelelectrophoresis

    Performsdataanalysisontestresultsfromvariousmolecularinstruments,reportingresultstolaboratorymanagerforreview

    mailto:dawn.jovan.coleman@gmail.com

  • Maintainsandorganizeslaboratorydatabasestosupportqualitycontrolrecordkeepinganddailyoperations

    Assistswithanalysisofgeneticmaterialderivedfromdiseaseagentsfoundinzoonotichostsandtestingmethods,ensuringthatalltestingcomplieswiththerequirementsoftheISO/IEC17025andtheA2LArequirements

    Supervisor:MilagrosSol(2539663152)OkaytocontactthisSupervisor:Yes

    WashingtonStateDepartmentofEcology300DesmondDriveSELacey,WA98503UnitedStates06/201509/2015Hoursperweek:25VolunteerDuties,AccomplishmentsandRelatedSkills:

    SortedmicroscopicbiomassesfromsedimentsamplesthatwerecollectedfromthePugetSoundwaters

    ProvidedresearchonknownbiomassesandenteredtheinformationintoaninternaldatabaseSupervisor:AngelaEagleston,MarineBenthicEcologist(3604076000)OkaytocontactthisSupervisor:Yes

    Education:TheEvergreenStateCollegeOlympia,WAUnitedStatesBachelor'sDegree06/2016CreditsEarned:182QuarterhoursMajor:MolecularBiologyMinor:BiochemistryRelevantCoursework,LicensesandCertifications:

    Separationofcomplexbiologicalspecimensviahandlingandinterpretationofgaschromatographymassspectrometry(GCMS),highpressureliquidchromatography,thinlayerchromatography(TLC),fouriertransforminfraredspectroscopy(FTIR)

    Researchincellbiologyandproteinbiochemistry Microbeextractionandisolation,microbestreaking,RealtimePCRandRTPCR,protein

    testingusingfluorescentphenylboronateagarosegelelectrophoresis IsolationofvirusesandDNAfrombacteriaandvertebrates(procuredfromTESCbiobank),

    useofrestrictionenzymes,andhybridizationtechniquesusingPCRtoidentifyallelesforgeneticmarkersandrepeatsequences

    RealtimereversetranscriptasePCR

  • Wetchemistrymethodsincludingtitrations,pH,compoundpurification,analysisandcharacterization,distillationandreflux

    Quantitativemethodsandexperimentaldesign Bioassays,laboratorybenchstudies,scientificreports,maintainingresearchlaboperationsand

    equipment Quantitativereasoningandscientificmathematics Descriptiveandinferentialstatistics Introductiontoforensicsciencewithlaboratory Microbialisolationofvirusesfromsoil ConventionalPCRandRTPCR Geneticsequencing,conventionalPCRconfirmationandorganismspeciation ExtractedDNAfrombovineheartandliver

    SPECIALPROJECTSSEAPHAGEprogramcoordinatedbytheHowardHughesMedicalInstituteandtheuseofbioinformaticstoolsonabacteriophagegenomeproject

    Collaboratedwithfacultycollectingsoilsamples,usingproperfieldmethodsinlocalareawithinterestinextractingasingularvirusstrain

    Overthecourseof10weeks,asoilsamplewasprocessedwithanenrichmentculture,filtered,diluted,plated,streaked,andpurified

    Determinedviraltiterstosuccessfullyisolateapurifiedbacteriophage WorkcontinuedwiththeisolationandcharacterizationofviralDNAbyelectrophoresisand

    restrictionenzymeanalysis

    SouthPugetSoundCommunityCollegeOlympia,WAUnitedStatesSomeCollegeCourseworkCompleted06/2013CreditsEarned:60QuarterhoursRelevantCoursework,LicensesandCertifications:

    AmericanSignLanguageI,II,III EnglishCompositionI,EnglishCompositionII,BasicWritingII BasicMathII,ElementaryAlgebra,BasicAlgebra,IntermediateAlgebra AnalyticalReading