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7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Djvkd Jttwecc
Depjrtie`t al @eurasnke`ne, Rfyskacagy & Rfjrijnacagy
U`kverskty Naccege Ca`da`
_egucjtka` al orjk` e`ergy suppcy=
wfere daes tfe jntka` oegk`.j`d e`d1
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Avervkew
Canus al na`trac al orjk` e`ergy suppcy -jrterkaces versus njpkccjrkes
Rerknytes nj` dkcjte njpkccjrkes
Faw ljst nj` tfks warh1 _ecevj`ne ta OACD lI_K
Ellent al ksnfjeikj a` njpkccjrkes _ace al perknytes k` cass al na`trac al orjk`
e`ergy suppcy k` pjtfacagy
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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ocaadlcaw
njpkccjry
siaatf
iusnce
e`datf
eckjcneccs perknytes
`eura`s
_egucjtka` al orjk` e`ergy suppcy oy
jrterkace siaatf iusnce vs perknytes
Nfjrces Ijrke Oe`mjik` _auget
(:6?4-:;24)
:67>
[ejr
:;42 :;52 :;22
>52
RuoIed= perknyte
RuoIed=
perknyte k` tktce
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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ocaadlcaw
njpkccjry
siaatf
iusnce
e`datf
eckjcneccs
:2 i
^I
:2 i
5 i
s
p5 i
s p
perknytes
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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_egucjtka` al njpkccjry dkjieter oy `arjdre`jck`e j`d gcutjijte
superlused a`ta nereoeccjr scknes
2
?2
42
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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ojshet
steccjte
grj`uce Gacgk
Rurhk`me
ocaad vessecs
k`put iassy lkores
autput
k`put
nckiok`g lkore
FoA?Fo
A?
LCAW
_ecevj`ne ta OACD lI_K
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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ojshet
steccjte
grj`uce Gacgk
Rurhk`me
ocaad vessecs
k`put iassy lkores
autput
k`put
nckiok`g lkore
FoA?Fo
A?
LCAW
_ecevj`ne ta OACD lI_K
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Deaxyfjeiagcaok` denrejses
ijg`etkn resa`j`ne skg`jcs
Deaxy-Fo ks pjrjijg`etkn
Kts ijg`etkn lkecd ijhes tfe tatjc ijg`etkn lkecd cess faiage`aus
Tfjt depfjses tfe prenesska` al prata`s k` tfe exter`jc lkecd (T?*denrejsed)
Tfjt k` tur` denrejses tfe skg`jc detented
^a iare deaxy-Fo iej`s cess skg`jc
OUT..
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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ojshet
steccjte
grj`uce Gacgk
Rurhk`me
ocaad vessecs
k`put iassy lkores
autput
k`put
nckiok`g lkore
FoA?Fo
A?
LCAW
gcutjijte
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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ojshet
steccjte
grj`uce Gacgk
Rurhk`me
ocaad vessecs
k`put iassy lkores
autput
k`put
nckiok`g lkore
FoA?Fo
A?
LCAW
\AC
gcutjijte
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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ojshet
steccjte
grj`uce Gacgk
Rurhk`me
ocaad vessecs
k`put iassy lkores
autput
k`put
nckiok`g lkore
FoA?Fo
A?
LCAW
\AC
gcutjijte
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Deaxy-Fo ks pjrjijg`etkn
Kts ijg`etkn lkecd ijhes tfe tatjc ijg`etkn lkecd cess faiage`aus
Tfjt depfjses tfe prenesska` al prata`s k` tfe exter`jc lkecd (T?*denrejsed)
Tfjt k` tur` denrejses tfe skg`jc detented
^a iare deaxy-Fo iej`s cess skg`jc
OUT..`eura`s k nrejse ocaad lcaw, j`d denrejse Xdeaxy-FoY sa
tfe lI_K skg`jc k`nrejses
Deaxyfjeiagcaok` denrejses
ijg`etkn resa`j`ne skg`jcs
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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OACD kijgk`g
Fjrkrk et jc. (?22?) ^nke`ne ?;7, 422
Jiygdjcj respa`se ta lejr stkiucus
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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\jsajntkve iedkjtars wkcc rejnf perknytes lkrst
RKJC U_LJNE
siaatf
iusnce
2tf arder
pe`etrjtk`g
jrterkace:st arder
njpkccjry
?`d ardernjpkccjry
>rd arder
njpkccjry
perknyte
:. Jntkve
neccs
?. Dkcjtars recejsed oy jntkve
`eura`s j`d jstranytes rejnf
perknytes oelare jrterkaces
`eura`
jstranyte
Hey
>. Rerknyte dkcjtka` ijy
sprejd ta jrterkace
J
OJJ
@A
RgE?
?2-FETE
ER4_
@A ocanhs praduntka` al na`strkntar ?2-FETE
dkcjtka`
na`strkntka`
A? Hi:2I
A? Hi>52I
I
GCU
@eura`s jre ~6.4 iknra`s lrai njpkccjrkes out 72 iknra`s lrai jrterkaces
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Ieiorj`e nurre`t nfj`ges evahed k` perknytes oy gcutjijte j`d `eura`jc JRs
Faw da vjsadkcjtary iesse`gers jllent perknytes1
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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@eura`jc jntkvkty evahes njpkccjry dkcjtka`
(stkiucjtka` al nereoeccjr pjrjccec lkores)
@eura`jc jntkvkty dkcjtes njpkccjrkes k` orjk` scknes wfjt joaut k` vkva1
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Wfknf vessecs respa`d lkrst k` vkva1
Naccjoarjtka` wktf Oadkc Gesscek` j`d Ijrtk` Cjurktze`, Nape`fjge`
ocaadlcaw
njpkccjry
siaatfiusnce
e`datfeckjcneccs perknytes
`eura`s
? pfata` kijgk`g k` vkva
:st arder
njpkccjry
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Njpkccjrkes dkcjte k` vkva
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Njpkccjrkes dkcjte oelare jrterkaces k` vkva
(wfksher pjd stkiucjtka`)
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Njpkccjrkes dkcjte oelare jrterkaces k` vkva
(wfksher pjd stkiucjtka`)
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Njpkccjrkes dkcjte oelare jrterkaces k` vkva
(wfksher pjd stkiucjtka`)
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Njpkccjrkes dkcjte oelare jrterkaces k` vkva
(wfksher pjd stkiucjtka`)
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Tfere jre iare, j`d cjrger, respa`ses `ejr perknytes
^ l d t
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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^uiijry al djtj
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Faw kipartj`t jre njpkccjry dkcjtka`s1
Njcnucjted reskstj`ne al
(k) pkj - cjyer 4 jrterkace
(kk) cjyer 4 jrterkace ta ve`uce
?22ui jwjy
(kkk) ve`uce pkj
_jtkas were 2.:=2.4=2.?
sa njpkccjrkes fjve iast
reskstj`ne
K`sertk`g aur iejsureddkcjtka`s suggests njpkccjrkes
ge`erjte 64% al tfe
k`nrejse al NOL (wfetfer ar
`at tfey recjx jntkvecy)
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Njpkccjrkes na`trkoute ta NOL k`nrejses k` twa wjys
RKJC ^U_LJNE
siaatfiusnce
2tf arder
pe`etrjtk`g
jrterkace:st arder
njpkccjry
?`d ardernjpkccjry
>rd arder
njpkccjry
perknyte
:. Jntkveneccs
?. Dkcjtars recejsed oy jntkve
`eura`s j`d jstranytes rejnfperknytes oelare jrterkaces
`eura`
jstranyte
Hey
>. Rerknyte dkcjtka` ijy
sprejd ta jrterkace
J
(:) Njpkccjrkes dkcjte lkrst j`d perknyte fyperpacjrkzjtka` ijy sprejd ta jrterkaces
(?) Tfe dkcjtka` evahed k` njpkccjrkes daik`jtes tfe denrejse al reskstj`ne ta lcaw k`
tfe nartex
A
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Avervkew
Canus al na`trac al orjk` e`ergy suppcy -jrterkaces versus njpkccjrkes - oatf
Rerknytes nj` dkcjte njpkccjrkes - vkj RgE?
Faw ljst nj` tfks warh1 - : sen _ecevj`ne ta OACD lI_K - perknytes k`ktkjte
tfe OACD skg`jc
Ellent al ksnfjeikj a` njpkccjrkes
_ace al perknytes k` cass al na`trac al orjk`
e`ergy suppcy k` pjtfacagy
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Jies et jc. (:;7
Laccawk`g ksnfjeikj, ocaad
lcaw retur`s ta jrterkaces out
saie njpkccjrkes ljkc ta lkcc a`
reperluska`
Wfkte jrejs relcent jrejs wktf `a perluska` laccawk`g
5 ik`s ksnfjeikj
Njpkccjrkes oenaie anncuded jlter ksnfjeikj
Na`
Ksnf
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Tfe perknyte respa`se ta ksnfjeikj
Dkjieter
(%)
Tkie (s)
2
:22
52
J`axkn
Depacjrksjtka`
?22 4222 ?s
j o
Reppkjtt, Fawjrtf, Iaoos & Jttwecc (?22, 722
>@j+
?H+JTR
>@j+
Nj?+
Nj?+
Nj?+
iyalkcjie`ts
praness jrau`d
njpkccjry
na`trac ksnfjeikj
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Tfe NOL respa`se ta ksnfjeikj
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Tfe NOL respa`se ta ksnfjeikj
Cellcer et jc., :;6;8 @ecsa` et jc., :;;?8 Fjunh et jc., ?224
-?2 2 ?2 42
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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@j+
?H+JTR
>@j+
Nj?+
Nj?+
Nj?+
iyalkcjie`ts
na`strknted
praness rau`d
njpkccjry
dejtf
Rerknytes ijy dke k` rkgar, pradunk`g j ca`g cjstk`g na`strkntka`Wfy da tfey dke1
Na`trac Ksnfjeikj2
?2
42
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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reperluska` tkie@IDJ_ ocanhers= 52 I JR5 + IH62: + 7-NH
JIRJ/HJ _ ocanhers= ?5 I @OZV
: I TTV
Gcutjijte recejse j`d reperluska` praiate perknyte dejtf
@knacj Fjikcta`, Njtferk`e Fjcc & Ncjre _ey`ecc, u`puocksfed
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Gcutjijte recejse j`d reperluska` praiate perknyte dejtf
@knacj Fjikcta`, Njtferk`e Fjcc & Ncjre _ey`ecc, u`puocksfed
INJA evahes perknyte dejtf k` vkva
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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INJA evahes perknyte dejtf k` vkva
Rerknytes dke jt j tkie wfe` e`datfeckjc neccs jre u`jllented
Naccjoarjtka` wktf Orjd ^utfercj`d j`d Jcjstjkr Ounfj`, k` Axlard
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Tfe NOL respa`se ta ksnfjeikj
Cellcer et jc., :;6;8 @ecsa` et jc., :;;?8 Fjunh et jc., ?224
-?2 2 ?2 42
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Avervkew Canus al na`trac al orjk` e`ergy suppcy -
jrterkaces versus njpkccjrkes - oatf Rerknytes nj` dkcjte njpkccjrkes - vkj RgE?
Faw ljst nj` tfks warh1 - : sen
_ecevj`ne ta OACD lI_K - perknytes k`ktkjte tfeOACD skg`jc
Ellent al ksnfjeikj a` njpkccjrkes - perknytesna`strknt j`d dke
_ace al perknytes k` cass al na`trac al orjk`
e`ergy suppcy k` pjtfacagy - nrunkjc, + lar OOO
Naccjoarjtars
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Naccjoarjtars
Ncjkre Reppkjtt Ncjre Fawjrtf Reter Iaoos
Njtferk`e Fjcc Ncjre _ey`ecc @khk Fjikcta` J`usfj Iksfrj Lergus ALjrrecc
Oadkc Gesscek` Ijrtk` Cjurktze` Orjd ^utfercj`d Jcjstjkr Ounfj`
CA@DA@
NARE@FJGE@
AVLA_D
_egucjtka` al orjk` e`ergy suppcy=
7/22/2019 David Attwell - Pericytes in neurovascular coupling in health and disease
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Jt njpkccjrkes!
_egucjtka` al orjk` e`ergy suppcy=
wfere daes tfe jntka` oegk`.j`d e`d1