33
Lake 2008 : Conservation and Management of River and Lake Ecosystem BASELINE STUDY OF AN River and Lake Ecosystem Dates : 22 nd - 24 th December 2008 Venue : Satish Dhawan Auditorium, Indian Institute of Science, Bangalore 560 012 BASELINE STUDY OF AN ECO-RESTORATION PROCESS ON A HYPER-EUTROPHIC URBAN WATER BODY HYPER EUTROPHIC URBAN WATER BODY Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther, CIM expert, CES, Anna University, Chennai K. Thanasekaran, Director, CES, Anna University, Chennai

Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

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Page 1: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Lake 2008 : Conservation and Management of River and Lake Ecosystem

BASELINE STUDY OF AN

River and Lake Ecosystem Dates : 22nd- 24th December 2008

Venue : Satish Dhawan Auditorium, Indian Institute of Science, Bangalore 560 012

BASELINE STUDY OF AN ECO-RESTORATION PROCESS ON A

HYPER-EUTROPHIC URBAN WATER BODYHYPER EUTROPHIC URBAN WATER BODYY. Prathishta, JRF, CES, Anna University, Chennai

Dirk Walther, CIM expert, CES, Anna University, ChennaiK. Thanasekaran, Director, CES, Anna University, Chennai, , , y,

Page 2: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

ContentsY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

Contents

Obj ti & Sit S l ti• Objectives & Site Selection

R t ti t h i• Restoration techniques

Eff t f t t t• Effect of treatment

C l i• Conclusion

Page 3: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

IntroductionY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

• Ramsar convention, 1971.

I di 15 illi b di 25 R i• India – 15 million water bodies, 25 Ramsar sites.

• “The National Lake Conservation Plan” (1994) and “TheNational Wetland Conservation Programme” (1995)National Wetland Conservation Programme (1995).

• “Wetland (Conservation and Management) rules 2008”.

• Hypereutrophic pond for the study.

• Technologies used for restoring the pond.

• Utilize the pond for propagation of wildlife.

Page 4: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

ObjectivesY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

jThe scope of the project is to developefficient eco-restoration mechanism in a

ll l h d hsmall scale eutrophic pond to reacholigotrophic state

• To Monitor the status of the pond by conducting Physico chemical and biological conducting Physico-chemical and biological analysis.

• Technologies to be urged for rejuvenating the pond by enhancing the oxygen loads the pond by enhancing the oxygen loads and maintaining the mass balance.

Page 5: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Study Area & Sampling Y. Prathishta, Dirk Walther, K. Thanasekaran (2008)

pStrategy

• The pond is located at Besant Nagar (Chennai, Tamil Nadu)

S.No

Frequency of sampling

Type of sampling

1. Before the t ti

Integrated li Tamil Nadu)

• Area - 2,452.8 m2

• Depth -1.6m l l 924 3

restoration process

sampling (H)

2. Aquatic h t

Integrated s li • Total Volume - 3,924 m3

• The pond was overgrown with (Pistia spp.) water

macrophyte removal

sampling (V)

3. During the filtering process

Integrated sampling ( pp )

cabbagesfiltering process sampling

(V)

V - VerticalV VerticalH - Horizontal

Page 6: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Sampling Sites

Y. Prathishta, Dirk Walther, K. Thanasekaran (2008)

DC

B

Sampling Sites 1.85 m

1.89 m

AE

C

1.71 m

1.9 m

2.5 m

Dissolved Oxygen(DO) Profiling with depth

DO in mg/L

0

0.2

0.4

0 1 2 3 4 5 6

DO in mg/L

0.6

0.8

1

1.2

Dep

th in

m

1.4

1.6

Site 'A' Site 'B' Site 'C' Site 'D' Site 'E'

Page 7: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

MethodologyY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

Pond

Aquatic macrophyte

removalStep 1 Reduces nutrient

concentration

Filtration and re-aerationStep 2 Accelerates and re-aerationStep 2

removes nutrients

Page 8: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Aquatic macrophyte removalY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

Aquatic macrophyte removal

Page 9: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Filtration and Re-aeration set upY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

Filtration and Re aeration set up

Page 10: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Impacts of RestorationY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

Impacts of RestorationBefore

restoration

E i E i M t

After water cabbage removal

During filtration

P t U it M t H E i HEpi- Epi- Meta-limnion limnion limnion

1 pH - 7.1 8.8 7.7 7.7 8.3 7.9 7.62 EC µS/cm 1390 957 950 954 381 375 3743 DO mg/L 3.2 4.4 3 1.2 4.8 3.9 1.9

Parameters Units Meta-limnion

Hypo-limnion

Epi-limnion

Hypo-limnion

S. No

g4 Temp ºC 24.7 30.6 29.5 29.2 31.1 29.2 29.15 TDS mg/L 922 1180 1250 1090 1283 1093 12876 TSS mg/L 131 257 149 210 140 1200 13957 TS mg/L 1050 1440 1200 1370 1423 107 1088 T bidit NTU 14 9 120 30 69 1 28 9 17 2 17 78 Turbidity NTU 14.9 120 30 69.1 28.9 17.2 17.79 NH4-N mg/L 15.4 BDL BDL BDL 1.4 0.8 1.1

10 NO2-N mg/L BDL BDL BDL BDL BDL BDL BDL11 NO3-N mg/L BDL 0.1 0.1 0.1 BDL BDL BDL12 TKN N /L 23 8 9 8 7 4 2 9 8 4 2 4 212 TKN-N mg/L 23.8 9.8 7 4.2 9.8 4.2 4.213 Chlorophyll a mg/m3 80.5 39 13.9 1.1 371 21.3 17.114 PO4

3- P mg/L 1.1 0.4 0.5 0.3 1.4 0.9 0.915 COD mg/L 168 96 92 86 45 45 9416 BOD (20ºC) /L 52 30 27 23 14 14 2816 BOD5(20ºC) mg/L 52 30 27 23 14 14 2817 Fecal coliform MPN/100ml 1.13*105 2.33*105 1.13*104 7.79*104 6.79*104 3.79*104 5.02*104

18 Total coliform MPN/100ml 1.13*105 1.13*105 6.79*103 5.22*104 5.02*104 1.13*104 3.79*104

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Y. Prathishta, Dirk Walther, K. Thanasekaran (2008)

Changes during restorationChange in water quality during restoration in metalimnionChange in water quality during restoration in epilimnion

3

7

0 5

3.9

0.8

4.2

0.91

234

56

78

conc

entr

atio

n in

mg/

L

3.2

15.4

23.8

4.4

9.8

4.8

9.8

5

10

15

20

25

once

ntra

tion

in m

g/L

0.150.5

01

DO NH4-N TKN PO43—P

parameters

c

after w ater cabbage removal during f iltration

3.21.10.15 0.41.4 1.4

0

5

DO NH4-N TKN PO43—P

parameters

c

before restoration after w ater cabbage removal during f iltration

Change in water quality during restoration in metalimnion

92

4360708090

100

on in

mg/

L

Change in water quality during restoration in epilimnion

371

250

300

350

400

n m

g/L

27

13.914

43

21.3

01020304050

BOD COD chlorophyll a

parameters

conc

entr

atio

after w ater cabbage removal during f iltration

52

168

80.5

30

96

3914

45

0

50

100

150

200

250

BOD COD chlorophyll a

parameters

conc

entr

atio

n in

after w ater cabbage removal during f iltrationbefore restoration after w ater cabbage removal during f iltration

Page 12: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Changes during restorationY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

Changes during restoration

Change in water quality during restoration in hypolimnion

1.2

4.2

0 3

1.9

1.1

4.2

0.91

1.52

2.53

3.54

4.5

once

ntra

tion

in m

g/L

DO profiling with depth

0 000 2 4 6 8

DO in mg/L

0.1 0.3

00.5

DO NH4-N TKN PO43—P

parameters

co

after w ater cabbage removal during f iltration

0.00

0.25

0.50

0 75m

Change in water quality during restoration in hypolimnion

86

94

60708090

100

n in

mg/

L

0.75

1.00

1.25

1.50

Dep

th in

m23

1.1

28

17.1

01020304050

BOD COD chlorophyll a

parameters

conc

entr

atio

f bb l d i fil i

1.75

2.00A E C D B

after water cabbage removal during filtration

Page 13: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

DO increaseY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

Production of oxygen during re-aerationFl 5 L/

DO increase

• Flow rate = 5 L/s• Volume of water per hour = 18 cubic meter• DO level in the pond = 0.79 mg/L• DO level in the outlet = 4 93 m/L• DO level in the outlet = 4.93 m/L• DO production in the outlet = 4.14 mg/L or 4.14g/m3

• Filtration process = 23.8 kg of O2

BOD reduction during aeration

• After water cabbage removal = 97.9 kg of BODf g g f• During filtration = 69 kg of BOD• Reduction of BOD = 28.9 kg of BOD• Reduction percentage = 30%

Page 14: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Effect of TreatmentY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

Effect of Treatment

Page 15: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Trophic Level IndexY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

pTrophic state

Nutrient enrichment category

Trophic Level Chla (mg/m3)

Secchi depth (m)

TP (mg/m3)

TN(mg/m3)

Ultramicro-trophic

Practically Pure 0.0 to 1.0 < 0.33 >25 < 1.8 <34

Micro- Very Low 1 0 to 2 0 0.33- 25 15 1.8- 34 73trophic Very Low 1.0 to 2.0 0.82 25-15 4.1 34-73

Oligo-trophic Low 2.0 to 3.0 0.82 -

2.0 15 - 7 4.1-9.0 73-157

Meso-trophic Medium 3.0 to 4.0 2 - 5 7-2.8 9 - 20 157-337

Eutrophic High 4.0 to 5.0 5 - 12 2.8-1 1 20-43 337-7251.1

Super-trophic Very High 5.0 to 6.0 12 - 31 1.1-

0.4 43-96 725-1558

HyperHyper-trophic Saturated > 6.0 > 31 < 0.4 > 96 > 1558

Source: Rotorua (2002)

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Status of the PondY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

Layers Chl a (mg/m3) OP (mg/m3)* TN (mg/m3) SD (m)

Epilimnion (BR) 80.5 1100 23800 1.01

Epilimnion (AMR) 39.0 400 9800 0.74

Metalimnion (AMR) 13.9 500 7000 0.86

Hypolimnion (AMR) 1.1 300 4200 0.77

E ili i (DF) 371 0 1400 9800 0 86Epilimnion (DF) 371.0 1400 9800 0.86

Metalimnion (DF) 21.3 900 4200 0.97

Hypolimnion (DF) 17 1 900 4200 0 96Hypolimnion (DF) 17.1 900 4200 0.96

Ultramicro-trophic

Micro-trophic

Oligo-trophic

Meso-trophic Eutrophic Super-

trophicHypereu-trophicp p

* TP > OP

BR – Before restoration, AMR – Aquatic macrophyte removal, DF – During filtration

Page 17: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Comparing techniquesY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

S.No

Aerators Oxygenproduction(kg/KWh)

Operationalcost (Rs)

Location

1.

2.3.

CP 150 (natural aeration)Surface low- speedSurface high speed

0.7

0.70.7

2,210

221+7000*221+7000*

Surface

SurfaceSurface

4.

5.

Submerged turbinewith sparger

Horizontal rotar

0.2

0.5

773.5+7000*

309.4+7000*

Submerged

Submerged

* H ti t

S.No

Aerators Cost perm3

Effect

* - Harvesting cost

1.2.3.4.

CP 150 (natural aeration)Surface low- speedSurface high speedSubmerged turbine with

0.670.060.060.23

Aeration &filtrationAerationAerationAeration

5.

gsparger

Horizontal rotar 0.09 Aeration

Page 18: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Achievements of restorationY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

Parameters Initial load (kg)

Load after aquatic macrophyte removal (Kg)

Efficiency%

Load during filtration (Kg)

Efficiency%

removal (Kg)BOD 204 97.9 52 69 66COD 659 326.2 51 225.7 66TKN 153 8 24 4 83 52 5 66TKN 153.8 24.4 83 52.5 66OP 4.3 1.3 70 3.9 9

Efficiency of the restoration process

5060708090

cy %

01020304050

Effic

ien

BOD COD TKN Orthophosphate

Parameters

Aquatic macrophyte removal During filtration& re-aeration

Page 19: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

ConclusionY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

Conclusion

• From the studies the pond is • Aeration in combination withfilt ti f bi i

pSuper-hypereutrophic (TLI)

• The average saturation level

filtration of biomass is aneffective technique inrestoring an hypereutrophicpond.g

of DO before and after therestoration processes werealmost doubled.

pond.

• Using similar aeration andfiltration technique will help

• Efficiency of bothtechniques in reducing the

f q pin restoring large scalewaterbodies.

techniques in reducing thenutrient concentration andrestoring a pond.

• Simultaneous harvesting ofalgae is effective inrestoring the pond

Page 20: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Future TreatmentY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

Future Treatment

• Harvesting of algae by (1) cutting or uprooting Harvesting of algae by (1) cutting or uprooting and removing the weeds from the pond.

• This step requires labour and cost, so further restoration steps should be taken to improve the

lit f th d quality of the pond.

• Probiotic consortium culture synergistically • Probiotic consortium culture synergistically works to inhibit the growth of pathogenic harmful bacterial through competitive exclusion. g p

Page 21: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,
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Dissolved Oxygen(DO) Profiling with depth

DO in mg/L

00 1 2 3 4 5 6

DO in mg/L

0.2

0.4

0.6

0.8

pth

in m

1

1.2

Dep

t

1.2

1.4

1 61.6

Site 'A' Site 'B' Site 'C' Site 'D' Site 'E'

Page 24: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

B

A

D

C

A

E

Page 25: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Change in water quality during restoration in epilimnion

23.8

20

25

L

15.415

20

n in

mg/

9.8

4 8

9.810

cent

ratio

3.21.1

4.4

0.15 0.4

4.8

1.4 1.4

0

5conc

0DO NH4-N TKN PO43—P

parameterspbefore restoration after w ater cabbage removal during f iltration

Page 26: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Change in water quality during restoration in epilimnion

371

350

400

250

300

in m

g/L

168

96150

200

entr

atio

n

5280.5

30

96

3914

4550

100

conc

e

0BOD COD chlorophyll a

tparameters

before restoration after w ater cabbage removal during f iltration

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Change in water quality during restoration in metalimnion

77

8

L

3 94.2

5

6

n in

mg/

L

3

3.9

2

3

4

cent

ratio

n

0.150.5

0.8 0.9

0

1

2

conc

0DO NH4-N TKN PO43—P

parameters

after w ater cabbage removal during f iltration

Page 28: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Change in water quality during restoration in metalimnion

92

8090

100

L

607080

n in

mg/

L

27

43

21.3304050

cent

ratio

n

13.914

01020

conc

0BOD COD chlorophyll a

parameters

after w ater cabbage removal during f iltration

Page 29: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Change in water quality during restoration in hypolimnion

4.2 4.2

44.5

L

2 53

3.5

n in

mg/

L

1.2

1.9

1.1 0.91.5

22.5

cent

ratio

0.1 0.3

0 9

00.5

1

conc

0DO NH4-N TKN PO43—P

parameters

after w ater cabbage removal during f iltration

Page 30: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Change in water quality during restoration in hypolimnion

86

94

90100

L

607080

n in

mg/

L

2328

304050

entr

atio

n

23

1.1

17.1

0102030

conc

0BOD COD chlorophyll a

parametersp

after water cabbage removal during filtration

Page 31: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Efficiency of the restoration processEfficiency of the restoration process

90

5060708090

cy %

20304050

ficie

nc

01020Ef

f

BOD COD TKN Orthophosphate

Parameters

Aquatic macrophyte removal During filtration& re-aeration

Page 32: Dates : 22 -24 Venue : Satish Dhawan Auditorium, Indian ...wgbis.ces.iisc.ernet.in/energy/lake2008/program/Lake2008...Y. Prathishta, JRF, CES, Anna University, Chennai Dirk Walther,

Changes during restorationY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

Change in water quality during restoration in epilimnion

Change in water quality during restoration in epilimnionesto at o ep o

23.8

20

25

mg/

L

p

371350400

mg/

L

15.4

9.8 9.810

15

20

ntra

tion

in

168

96

257

13180 5

140150200250300350

atio

n in

m

3.21.1

4.4

0.4

4.8

1.40.15 1.4

0

5

10

conc

e 523096

391445

80.5

050

100150

BOD COD chlorophyll TSSco

ncen

trDO NH4-N TKN PO43—P

parameters

BOD COD chlorophylla

TSS

parametersbefore restoration after w ater cabbage removal during filtrationbefore restoration after w ater cabbage removal during f iltration before restoration after w ater cabbage removal during f iltration

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Changes during restorationY. Prathishta, Dirk Walther, K. Thanasekaran (2008)

Change in water quality during restoration in metalimnion

Change in water quality during restoration in metalimnion

7

3 9 4 25678

in m

g/L

92

149

107125150175

on in

3

0 5

3.9

0.8

4.2

0.92345

cent

ratio

n

27

92

14

43

13.9 21.3255075

100

conc

entra

tim

g/L

0.50 80.15

01

DO NH4-N TKN PO43—P

parametersco

n

025

BOD COD chlorophylla

TSS

c

parameters

after water cabbage removal during filtration

parameters

after water cabbage removal during filtration