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Conferenza di Carlo Alberto Redi "Il futuro delle cellule staminali" in occasione della Mostra Darwin 1809-2009
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CarloAlberto RediAccademia dei Lincei
Università degli Studi di PaviaFondazione IRCCS
Policlinico San Matteo - [email protected]
Il futurodelle
cellule staminali
Milano 16 luglio 2009Palazzo Besanaore 18.30
Diagramma di Valentin Häcker (1892)con cellule staminali nell’embrione di Cyclops
Diagramma di Artur Pappenheim (1905) per illustrare la ematopoiesi
Stem cell basic biology
neonato
adulto
Crescita e differenziamento
fecondazione
sviluppo embrionale
zigote
oocita
sperm
meiosi
meiosi
Lialm Donaldson report, June 2000
SCAFFOLDS, CELLULE STAMINALI EBIOREATTORI PER LA PRODUZIONE DITESSUTO OSSEO BIOINGEGNERIZZATO
F. Benazzo and the components of CIT
SEM 450 XAnti-osteocalcin
Punched Titanium
SEM 50 X
surviving graft 10 ye ars after transplantationof human fetal dopamine neurons to the right
striatum
Positron emission tomography scan Olle Lindvall, “Stem cell transplantation”
Variazione della captazione di 18F-fluorodopa nei cervelli di pazienticon malattia di Parkinson dopotrapianto, dimostrato dall‘esamePET
1 106
!! Cytoplast !!
!! Cytoplast !!
TELOMERETELOMERE’’ LENGTH AT DIFFERENT TIME LENGTH AT DIFFERENT TIMEPOINTS OF EXPANSIONPOINTS OF EXPANSION
WEEKS OFCULTURE
00 17.7 17.7 8.8 8.8+2+2 21.1 21.1 10.4 10.4
+4+4 20.7 20.7 10.2 10.2
+5+5 18.6 18.6 9.8 9.8
+6+6 18.4 18.4 9.1 9.1
KMESF KMESF KbKb
(FLOW-FISH)(FLOW-FISH)
Yamanaka and coworkers (2006/07): ≈1 on 5,000 ≈ 0.02% Thomson and coworkers (2007): ≈ 1 on 10,000 ≈ 0.01% cytoplasts (insulinoma & stem cell extracts)Collas and coworkers (2004): ≈1 on 2 ≈ 50%Redi and coworkers (2007): ≈ 1 on 3,000 ≈ 0.03%
retrotransfections (stemness & oncogenes)
! ! !biotechnology
cloningstem cells
NO
stem cells
objections to reproductivebiotech
We should not play God
Henrietta Lacks 1920 - 1951
HeLa cellsHeLa cells should be given the new speciesname of Helacyton gartleri.The cells are a genetic chimera of HPV18 andhuman cervical cells and now have a distinct,stable, non-human chromosome number.
HeLa cellsHeLa cancer cells have evolved into a self-replicating, single-cell life-form.
HeLa cellssince 1951 self-replicatingused in thousands biol expcontribution to medicine