CVM/COP Research Collaboration CVM/COP Research Collaboration SymposiumSymposium

What CVM has to Offer-

“Neuroscience” Al Beitz

Neuroscience Investigators – Neuroscience Investigators – Basic ScienceBasic ScienceAl Beitz – pain, analgesia, acupunctureDavid Brown – Gut neurobiology, immunityMaxim Cheeran- Viral EncephalitisJohn Collister - CNS regulation during

hypertension and heart failure Alice Larson – pain and analgesia, stressPam Skinner – neurodegenerative diseasesLucy Vulcanova-Hart – pain and analgesia,

pain and the gut microbiome

Neuroscience Investigators – Neuroscience Investigators – Clinical ScienceClinical ScienceHwa Choi – AcupunctureElizabeth Goudie DeAngelis – Pain

and AnalgesiaLynelle Graham – pain

management, acupunctureMike Hanson – Cancer PainAngie Jimenez – pain recognition

and managementNed Patterson – EpilepsyKerry Robinson – Anesthesiology,

Pain and Analgesia

Animal ModelsAnimal ModelsRodent model of FibromyalgiaRodent models of Cancer, Neuropathic,

Inflammatory, Visceral and Arthritic Pain

Rodent models of hypertension and heart failure – CNS effects

Mouse model of prion diseasePrimate model of HIVMurine model of Herpes Simplex

EncephalitisNaturally occurring Canine and Feline

models of CNS disease and pain conditions

Expertise:Expertise:- neuroanatomy- microperfusion techniques- fluorescence immunohistochemistry- confocal microscopy; calcium imaging- primary sensory neuron culture- proteomics- flow cytometry- behavioral techniques – pain testing, Morris water maze, activity boxes- telemetry data acquisition system –BP- echocardiography - stress hormone measurement- neuroimmune measurements

EquipmentEquipmentOlympus FluoView 1000 confocal

microscope Radiotelemetry to continuously

measure arterial pressure and heart rate in conscious, freely moving, rats

Behavioral apparatus for pain testing; activity boxes; rotarod for motor skill accessment; Forced swim test

Metabolic Cages

Example of successful Example of successful collaboration: Al Beitz and collaboration: Al Beitz and Phil PortoghesePhil PortoghesePhil synthesized several bivalent

ligands with a mu opioid receptor agonist end and a metabotropic glutamate receptor antagonist end

Our lab tested these bivalent ligand in a mouse inflammatory pain model and a cancer pain model

One of the bivalent ligands proved to be extremely potent in both pain models

Paper submitted to PNAS