Embed Size (px)
Citation preview
Amanda Thomas [email protected]
ADDRESS 7507 Sagamore Road, Prairie Village, Kansas 66208 USA (316) 772-8615 OBJECTIVE To obtain a position in the field of medical research. EDUCATION Kansas State University, Manhattan, Kansas Bachelor of Science May 2007 Graduate Major: Microbiology GPA 3.438/4.0 CAREEREXPERIENCE Children’s Mercy Hospital, Kansas City, Missouri
Clinical Trials Coordinator I February 2014–Present• Regulatory Document Maintenance • Study Coordination • Subject Consents• Database Management • Record Documentation • Billing Maintenance• Sample Processing • Protocol Creation/ImplementationChildren’s Mercy Hospital, Kansas City, MissouriResearch Associate in Clinical Pharmacology October 2014 – Present• CYP2D6 Characterization • Array Development • Manuscript writing
• Sanger Sequencing • Copy Number Variation • Supply Ordering
• XL-PCR analysis/development • DNA Sequence Analysis • PCR Analysis• RNA/DNA Extraction (Blood/Saliva/Tissue) • Supply Ordering• HRM Analysis • Agarose Gel Electrophoresis • Personnel Training
• Genotyping (RFLP/TaqMan/Sanger) • BSL II Trained • Assay Development• Equipment Maintenance • Study design/implementation
Research Assistant II in Clinical Pharmacology May 2008 - Oct 2014• HepG2/A549 Cell Maintenance • Transformation and Ligation • Manuscript writing
• Transient Transfections • Prepping Cultures • RNA Quality analysis
• Nuclear/Cytostolic Extractions • DNA Sequence Analysis • PCR Analysis• RT-qPCR Design/Analysis/MIQE • TOPO TA Cloning • Supply Ordering• Western Blotting • RNA/DNA Extraction (Blood/Saliva/Tissue)
• Agarose Gel Electrophoresis • Chromatin Immunoprecipitation• HRM Analysis• Genotyping (RFLP/TaqMan/Sanger) • Tissue Inventory Maintenance • Copy Number Analysis• ELISA Analysis • BSL II Trained • Assay Development
Stowers Institute for Medical Research, Kansas City, MissouriResearch Technician in a Drosophila Laboratory May 2007 – May 2008• Fly Stock Maintenance •Transformation and Ligation • Microscopy• Embryo Fixations • Prepping Cultures • Cloning• Fluorescent/Colorimetric Staining • DNA Sequence Analysis • Mutagenesis Screen• DNA Transfection • Competent Cell Preparation • DNA Extraction• S2 Cell Maintenance • Protein Gel Analysis • PCR Analysis• Agarose Gel Electrophoresis • Plasmid Stock Maintenance • Genetics• Restriction Digest and Analysis • General Lab Maintenance
Kansas State University, Manhattan, Kansas August 2004 – May 2007 Research Technician in Fungal Ecology Laboratory
• Aseptic Technique • PCR analysis
• DNA Extraction • DNA Sequencing and Analysis• RFLP Digest and Analysis • Agarose Gel Electrophoresis• Plasmid Cloning and Screening • Clone Library• Microscopy • Maintaining Cultures• Research experience in Alaska and Cascade Mountains, WashingtonResearch Experience for Undergraduates June – August 2006• Long Term Ecological Research Grant through Konza Prairie Manhattan, Kansas• Root endophyte colonization of Arabidopsis thalianaAttended International Conference of Mycorhizae July 2006• Granada, SpainKansas State University, Manhattan, Kansas Sept. 2006 – May 2007Academic Learning Center Tutor• Spanish • Sociology
SKILLS/COURSES Computer Programs • Word • Vector NTI • INFOR (Lawson)• Excel • Sequencher • Quartzy• Power Point • Access • REDCap• PHASE • geNorm • FreezerWorks• Norm Finder • Best Keeper • SPSS • Entrez/BLAST • PHRED • MARS
Relevant Course Work• Modern Genetics • Cell Biology• Molecular Genetics Lab • Immunology• Genetics of Microorganisms • Cancer Cell Biology• SPSS 101/102 • Interpersonal Communication• Exploring a Leadership Role • Leading without Authority
Language• Spanish – Conversational and Written
PUBLICATIONS In Print
● Gaedigk, Andrea, Amanda K. Riffel, Belen Garcia Berrocal, Virginia Garcia Solaesa, Ignacio Davila, Maria Isidoro-Garcia. “Characterization of a complex CP2D6 genotype that caused an AmpliChip CYP450 Test no-call in the clinical setting.” Clinical Chemistry and Laboratory Medicine 52.6 (2014): 799-807.
● Gaedigk, Andrea, Natalie Freeman, Toinette Hartshorne, Amanda K. Riffel, David Irwin, Jeffrey R. Bishop, Mark A. Stein, Jeffery H. Hewcorn, Lazara Karelia Montane Jaime, Mariana Cherner, J. Steven Leeder. “SNP genotyping using TaqMan technology: the CYP2D6*17 assay conundrum.” Nature Scientific Reports 5:9257 (2015)
● Jones, Bridgette L., Belinda K. Graham, Amanda K. Riffel, Hongying Dai, Lanny J. Rosenwasser, Carrie A. Vyhlidal. “Genetic Variation in the TNFA Promoter Region and TNFA Gene Expression in Subjects with Asthma.” Journal of Asthma 50.6 (2013): 541-547.
● Vyhlidal, Carrie A., Amanda K. Riffel, Hongying Dai, Lanny J. Rosenwasser, Bridgette L. Jones. "Detecting Gene Expression in Buccal Mucosa in Subjects with Asthma vs Subjects without Asthma." Pediatric Allergy and Immunology 24.2 (2013): 138-143.
● Vyhlidal, Carrie, A., Amanda K. Riffel, et al. “Cotinine in Human Placenta as a Biomarker of In Utero Cigarette Exposure.” Drug Metabolism and Disposition 41.2 (2013); 305-311.
● Jones, B.L., L. Rosenwasser, A.K. Riffel, B.K. Graham, C.A. Vyhlidal. “Buccal Mucosal Sampling is a Reliable Method to Determine Differences in Gene Expression Patterns between
Subjects with Asthma Compared to Health Controls” Journal of Allergy and Clinical Immunology 127.2 (2011): AB213.
● Walker, J. F., L. Aldrich-Wolfe, A. Riffel, H. Barbare, N. B. Simpson, J. Trowbridge, and A. Jumpponen. "Diverse Helotiales Associated with the Roots of Three Species of Arctic Ericaceae Provide No Evidence for Host Specificity." New Phytologist 191.2 (2011): 515-27.
● Riffel, Amanda K., Eric Scheuneman, and Carrie A. Vyhlidal. "Regulation of the CYP3A4 and CYP3A7 Promoters by Members of the Nuclear Factor I Transcription Factor Family." Molecular Pharmacology 76.5 (2009): 1104-114.
In Progress● Gaedigk, Andrea, Amanda K. Riffel, Natalie Freeman, Toinette Hartshorne, Jeffrey R Bishop,
J Steven Leeder..”SNP genotyping using TaqMan technology: the CYP2D6*17 assay conundrum.”
● Vyhlidal, Carrie A., Amanda K. Riffel, Shinya Ito. "Developmental Expression of NFAT5 in Prental Human Liver."
Posters● Gaedigk, Andrea, Hye-Eun Jeong, Carles Garcia-Ribera, Amanda Riffel, Belen Garcia-
Berrocal, Virginia Garcia Solaesa, Ignacio Davila, Maria Isidoro-Garcia, Jae-Gook Shin, Juanjo Hernandes-Sanchez. “Characterization of AmpliChip CYP450 test no-calls in the clinical setting: identification of novel CYP2D6 variants.”
● Repnikova, E., Fiedler, S., Riffel, A.K., Frerking, A., Joyce, J., Nick, J., Thakor, P., Zhang, L., Cooley, L. and Gaedigk, A. (2015). Characterization of copy number variations involving CYP2D6 locus detected by various microarray platforms. Annual Clinical Genetics Meeting (ACGM), Salt Lake City, UT, March 24-28 2015, abstract #740
● Vyhlidal, Carrie A., Amanda K. Riffel, Shinya Ito. "Expression of NFAT5 in Human Fetal Liver: Correlation with CYP3A Expression."
● Schuenemann, Eric, Amanda K. Riffel, Carrie A. Vyhlidal. "Regulation of Human CYP3A4 and CYP3A7 by the NFI Transcription Factor Family."
● Riffel, Amanda, Nicholas B. Simpson, Justin Trowbridge, Chad B. Fox, Keerthi Mandyam, Ari Jumpponen. "Arabidopsis thaliana Colonization by Ascomycetous Root Endophytes."
LEADERSHIP • Volunteer animal care The Pet Connection January 2010 - present
• Volunteer animal care Unleashed Pet Rescue and Adoption March 2012 - July 2012• Big Sister in Big Brother Big Sister program May 2010 - present
• Microbiology Club Treasurer August 2006 – May 2007 • Volunteer tutor for 5th Grade Students August 2004 – May 2006 • Big Sister in Big Brother Big Sister program August 2002 – May 2003 • National Young Leaders Conference Washington, DC October 2002 HONORS & • June Hull Sherrid Scholarship • Circle Kay Club • Microbiology Club ACTIVITIES 2003 – 2007 Aug. 2003 – May 2005 Aug 2003 – May 2007 • Sjogren Scholarship • Habitat for Humanity 2003 Aug. 2004 – May 2005 • American Legion Scholarship • Outstanding Student: Mathematics and Science 2003 2003 • Catbackers Scholarship • Girls Researching Our World Presenter Newton, Kansas Chapter 2003 Dec. 2005 & Jan. 2006 • Kansas Honors Scholar • KSU Telefund Caller 2002 2003-2007
