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Protein Ark Ltd, The Innovation Centre, 217 Portobello, Sheffield S1 4DP, UK.
For a quotation or further details contact us on:
Telephone:+44 (0) 33 33 44 20 25
Email: [email protected]
or visit our website: www.proteinark.com
About Us
Protein Ark was founded in 2014 and brings together 15 years experience in contract manufacturers and custom services in the UK. Our PhD qualified scientists will provide our customers with the highest quality customised products, services and support.
Facilities
Sheffield: Head office manufacturing, production, testing and technical support
Expertise
Peptide Synthesis Antibody development Molecular biology Protein expression Protein purification Large scale production Analytical technologies
Our guarantee
High quality service Regular updates Competitively priced Scheduled delivery times Troubleshooting and
problem solving Dedicated management
team
Custom Services
Peptide Synthesis
Polyclonal Antibodies
Monoclonal Antibodies
Cloning
Recombinant Protein Services
Bulk Production Services
Antibody production
Protein Ark provides a high quality custom peptide synthesis service with expertise in modified and difficult peptides. Peptide Service: Sequence: 2 to 100 amino acids Purity: Crude to >98% Quantity: Milligrams to 100 grammes Quality: Full mass spec and HPLC analysis Time scale: 2-3 weeks Guarantee: No peptide no fee
Modified and difficult peptides Hydrophobic peptides Non-standard amino acids:
Phosphorylated, D-isomers, methylated, non-natural
Spacers and linkers: PEG, AVA, GABA, AEA, Gly, ANP
Labels: Biotin, FITC, 6-FAM ...and more Cyclic peptides: Disulphide or amide
terminal/side chains N & C terminal Peptide antigens for antibody production Conjugations: KLH, BSA and OVA All our peptides are competitively priced with fast turnaround times and excellent customer support. See our ONLINE ENQUIRY FORM for further details.
Peptide synthesis
Protein Ark provides a fully customised immunisation and antibody production service to meet your needs. Monoclonal Antibody Antigen: Peptide or protein (from
sequence) Peptide: Design, synthesis, conjugation Species: Balb/c Mice Immunisation schedule: 45 day Hybridoma fusion, cloning and cell
banking Screening: ELISA, end-point assay Production: Cell culture Purification: Protein G, affinity
chromatography Deliverable: 2-5 mg purified antibody
and Hybridoma cell line Time scale: 5-6 months Scalability: Gramme quantities
Polyclonal Antibody Antigen: Peptide or protein Peptide: Design, synthesis, conjugation Species: Rabbit, Rat, Guinea Pig Immunisation schedule: 69 day Purification: Protein G, affinity
chromatography Deliverable: 2-10 mg purified antibody Time scale: 3-4 months Guarantee: No antibody no fee
Additional antibody services Phospho-antibody production Testing: WB, IHC, IP Protein free cell line adaptation Fragmentation: Fab and Fab’ generation Produced by experts to ensure the final product is of the highest quality. See our ONLINE ENQUIRY FORM for further details.
Cloning
Protein production
With dedicated teams and extensive experience our UK based facility provides a wide range of high quality services. Expression Service Platform: Bacterial, insect (baculovirus
and stable), mammalian (transient and stable), hybridoma
Screening: strains, clones, media Trial expression: < 0.5 litres Small scale expression: < 10 litres Large scale expression: 50-250 litres Analysis: SDS-PAGE, Western blot
Purification service Un-tagged Tag removal: TEV, enterokinase, thrombin Affinity: polyHis, GST, Biotin, Protein A/G Ion-exchange and size-exclusion
chromatography
Additional services Method development Labelling: Selenomethionine,
selenocysteine, N15
Validated ELISA assay development Endotoxin removal from protein sample Fragmentation: Fab and Fab’ generation Cell line banking and storage
Time scale: 1 litre expression and purification Bacteria: 2-3 weeks Insect cells (baculovirus): 4-5 weeks Insect cells (stable): 6-7 weeks Mammalian: 6-7 weeks
All our production services are performed to ISO 9001 standards to ensure the final product is of the highest quality. See our ONLINE ENQUIRY FORM for further details.
Protein Ark’s dedicated molecular biology team provides a broad range of cloning services and proprietary vectors for successful recombinant protein expression. Cloning Service: Codon optimization and design service Gene synthesis (using GeneWiz) Standard and non-standard restriction
enzyme cleavage Clone into any vector of your choice:
including all standard or licence free vectors
Tags: polyHis, GST, FLAG, V5, Myc, FMDV Time scale: 2-3 weeks Quality assured and sequence verified
Licence free proprietary vectors Based upon same core plasmid
backbone Available for bacterial, insect, and
mammalian expression Standard or custom designed Dual expression versions Modular design Choice of 156 individual components
including: promoters, reporters, antibiotic resistance, origins, tags, signal peptides and more
Save time and money with our fast turnaround sequence verified cloning service. See our ONLINE ENQUIRY FORM for further details.