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Peptidomimetics
Post TranslationalModification
Multi-disulfidePeptides
PeptideSynthesis
Multi-stepOrganic
Synthesis
Peptide- Protein
Conjugates
Peptide Derivatives
Your Peptides International Custom Synthesis Team Leaders
Two key advantages, support and expertise, are exemplified by the custom peptide team
Production
Michael Pennington, Ph.D.President, Chief Executive Officer
Dr.Penningtonhasover20yearsinresearchand development management and is theauthor of nearly 100 technical publications.He has served on the editorial boards ofseveral industry journals and is on theScientificAdvisoryBoardsofanumberofdrugdevelopmentcompanies.
Dr. Satendra Chauhan, Ph.D.Chief Operating Officer
Dr.Chauhanhasextensiveexpertiseinretro-synthetic analysis, synthesis, purificationandanalysisofcomplexorganicmolecules,peptides and peptidomimetics as well astotal synthesis of natural products. Dr.Chauhanhasoverthreedecadesofpeptidelaboratoryexperience.
Andrzej Czerwinski, Ph.D.Senior Chemist
Dr.Czerwinski’scareerinpeptidechemistryspans over 40 years and he has been avital PI member for well over a decade.With numerous peer-reviewed papers andarticles to his credit, Dr. Czerwinski bringsliterally a lifetimeof experienceand insighttoeachproject.
Quality Assurance Quality Control
Patricia Coxon Ph.D. Quality Assurance
Dr. Coxon assures a strict adherance to qualitystandards while expediting smooth projectmanagement. She serves as liason between thecustomer and PI laboratory, securing a stress-freerealizationforeachcustomproject.
Yingjun Zhang, M.S.Quality Control
YingjunZhang is thefinalauthority forqualitycontrol,certifying that the data matches the requestedspecifications,foreachcustompeptideproducedinthePIlaboratory.
Custom Peptide & Chemistry Services
Peptides International has made a major commitment in personnel and equipment that significantly increases the breadth of its technology in peptide science. These resources, together with the company’s broad background in solving custom peptide challenges , now offers researchers a COMPLEX CUSTOM PEPTIDE TECHNOLOGY PLATFORM (see front) from which to develop new projects. The platform assures an integrated company-wide effort that not only meets researcher’s immediate synthetic needs, but can offer additional services in areas such as analytical testing, technology/process development, and scale-up. Examples of several elements of the platform are described below. Our team of scientists (see back) has expertise and experience in each of the areas of the platform and welcomes the opportunity to consult with researchers at any point in the development of their projects.
Product Quality and Value • Strict Confidentiality • Proactive Project Management • Friendly Service
The Complex Custom Peptide Technology Platform
Multi-disulfidePeptides
Lys-22
Shk
Arg-1
Linker
FITC
Biotin
TMR
Peptides International was established in 1983 and is located in Louisville, Kentucky. The PI laboratories, updated and expanded in 2007, consist of well-equipped R&D labs, a pilot plant with 100-liter reactor system, in-process control instrumentation, purification systems and high-capacity freeze dryers. Incoming raw materials, as well as finished goods, are analyzed using state-of-the-art methods including HPLC, MS, AAA, UV and FTIR spectroscopy, polarimetry, and traditional organic analytical techniques. With these capabilities and the experience of its staff, Peptides International is able to offer effiecient and cost effective solutions to serve the challenging needs of modern peptide-based drug discovery and development.
Interested in mini-proteins with multiple disulfide bonds? PI researchers have extensive experi-ence folding these highly complicated molecules to their biologically active forms. Examples
include native molecules and analogs of conotoxins, defensins, hepcidins,
chemokines, ion channel blockers (ShK, HwTX4, cha-
rybdotoxin, iberiotoxin, marga-toxin, ProTx-II, mu-conotoxins, etc).
They can also customize with labels such as TAMRA, FITC or Biotin using a mini-
PEGTM spacer (example at right).
• Hydroxyethylene Isostere
• Reduced Peptide Bonds
• Aldehydes
• Chloromethylketones
• Depsipeptides
• Phosphonates
• Glycosylation
• Phosphorylation
• Fatty Acylation
• Pegylation
• Cyclization
• Disulfide Bonds
• Novel Amino Acid Derivatives
• Stereo Selective Synthesis
• Special Protecting Groups
• Natural Product Synthesis
• Chromogenic / Fluorogenic Substrates
Peptide-protein conjugates have a variety of applications, including the stimulation of antibodies for immunization
(see KLH (Keyhole Limpet Hemocyanin) example at right). These conjugates can be customized as
needed, including combinations with pro-teins such as Albumin, Ovalbumin, and Ferritin. Selective heterobifunctional
crosslinkers allow for selective attachment of a Cys-containing peptide to the protein of
your choice. Using KLH-MCC will help streamline this reaction, and the product (KLH-6000-PI) is conve-
niently kept in stock at all times.
Peptide-ProteinConjugates
NH
O
N
O
O
NH
O
N
O
O
S
SH Peptide
Peptide
KLH
KLH
MALEIMIDE-ACTIVATED CARRIER PROTEIN
CARRIER-PEPTIDE CONJUGATE
SULFHYDRYL-CONTAINING HAPTEN (PEPTIDE WITH
TERMINAL CYSTEINE)
Fluorescence resonance energy transfer (FRET) occurs when excitation energy is transferred from an excited fluorescent donor to a quenching acceptor in a distance-dependent manner. Cleavage of a scissile peptide bond within a fluorescence quenched substrate leads to separation of the intramolecular donor-acceptor pair, thus allowing an increase in fluorescence. The fluorescence increase is proportional to the amount of peptide hydrolyzed. If you need to design a substrate for a newly discovered enzyme or application, contact our specialists to begin your Substrates by DesignTM project today.
Peptide Derivatives
Post TranslationalModification
• Classical/Solution
• Solid-Phase
• Native Chemical Ligation
• Dendrimeric RGD Platforms
Peptidomimetics
Multi-stepOrganic
SynthesisPeptide
Synthesis
hν
QUENCHER
DABCYL
ENZYME
EDANS
DONOR
GENERATION OF FLUORESCENT SIGNAL
EFFICIENT QUENCHING OF DONOR FLUORESCENCE
DABCYLEDANS
Contact us for additional examples in these areas
CATALOG PRODUCTS• RGDPlatform• ProTx-II&OtherToxins• BiologicallyActivePeptides• Substrates&Inhibitors• [email protected]
CUSTOM CHEMISTRY SERVICESMadeinUSA• DesignConsultation• ChemistryDevelopment• ProcessScale-up• Pilot-ScaleSynthesis• Solution&SolidPhase• SubstratesbyDesignTM
TM
KeyliteraturecitationsandpatentsreferringtoPeptidesInternationalproductsandservices
EXCELLENCE COMPOUNDED• HighPurity,QualityandValue• StrictConfidentiality• FriendlyService• ProactiveProjectManagement
PEPNET.
Your Peptides International Custom Synthesis Team Leaders
Two key advantages, support and expertise, are exemplified by the custom peptide team
Production
Michael Pennington, Ph.D.President, Chief Executive Officer
Dr.Penningtonhasover20yearsinresearchand development management and is theauthor of nearly 100 technical publications.He has served on the editorial boards ofseveral industry journals and is on theScientificAdvisoryBoardsofanumberofdrugdevelopmentcompanies.
Dr. Satendra Chauhan, Ph.D.Chief Operating Officer
Dr.Chauhanhasextensiveexpertiseinretro-synthetic analysis, synthesis, purificationandanalysisofcomplexorganicmolecules,peptides and peptidomimetics as well astotal synthesis of natural products. Dr.Chauhanhasoverthreedecadesofpeptidelaboratoryexperience.
Andrzej Czerwinski, Ph.D.Senior Chemist
Dr.Czerwinski’scareerinpeptidechemistryspans over 40 years and he has been avital PI member for well over a decade.With numerous peer-reviewed papers andarticles to his credit, Dr. Czerwinski bringsliterally a lifetimeof experienceand insighttoeachproject.
Quality Assurance Quality Control
Patricia Coxon Ph.D. Quality Assurance
Dr. Coxon assures a strict adherance to qualitystandards while expediting smooth projectmanagement. She serves as liason between thecustomer and PI laboratory, securing a stress-freerealizationforeachcustomproject.
Yingjun Zhang, M.S.Quality Control
YingjunZhang is thefinalauthority forqualitycontrol,certifying that the data matches the requestedspecifications,foreachcustompeptideproducedinthePIlaboratory.
Peptides International, Inc.11621ElectronDriveLouisville,KY40299USA
phone:+1-502-266-8787tollfreephone:+1-800-777-4779(USandCanada)
fax:+1-502-267-1329email:[email protected]
InCalifornia,pleasecontactSmitaYadavphone:+1-858-361-4175
email:[email protected]
Our Legacy
Arno Spatola, Ph.D.Founder
PeptidesInternational
Dr. Arno Spatola, an internationally recognized peptidechemistryresearcher,foundedPeptidesInternationalasanoutgrowth of his research interests. UnderDr.Spatola’sguidance,aseriesofsignificanteventshelpeddevelopthecompanyintowhatitistoday.ThealliancewiththePeptideInstituteofOsaka,Japan–whichcontinuestothepresent–hasallowedPeptides International to offer thepremiumproductsofthisrenownedinstitutedirectlytoitscustomers.Custom synthesis, as described here, has become anintegralpartof thecompany’sportfolio. Goingforward,PIhonorsthelegacyofDr.Spatolabysteadfastlyadheringtothevaluesonwhichitwasfoundedwhilekeepingitsfocusonthefutureofpeptidescience.
