CURRICULUM VITAE PERSONAL DATA · CURRICULUM VITAE PERSONAL DATA Name: Bruce S. Klein, M.D. Office Address: Division of Infectious Diseases . Center for Health Sciences, Room K4/434

CURRICULUM VITAE

PERSONAL DATA Name: Bruce S. Klein, M.D.

Office Address: Division of Infectious Diseases Center for Health Sciences, Room K4/434 University of Wisconsin-Madison Madison, WI 53792-4108 (608) 263-9217

EDUCATION

1974 Political Science Magna Cum Laude Phi Beta Kappa (1973) Boston University College of Liberal Arts B.A.

1978 Alpha Omega Alpha

Boston University School of Medicine M.D.

POSTGRADUATE EDUCATION:

1978-1981 Pediatric Intern, Assistant and Senior Resident Department of Pediatrics Johns Hopkins Hospital

1984-1986 Fellow in Infectious Disease Departments of Pediatrics and Internal Medicine Sections of Infectious Disease University of Wisconsin Hospital and Clinics

PROFESSIONAL APPOINTMENTS:

1981-1983 Epidemic Intelligence Service Officer Assigned to Wisconsin Division of Health

4/9/12 Bruce Klein, MD

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Field Services Division, Epidemiology Program Officer, Centers for Disease Control U.S. Public Health Service

9/86-6/90 Instructor

Departments of Pediatrics and Internal Medicine Sections of Infectious Disease University of Wisconsin Medical School Madison, WI

7/90-2/95 Assistant Professor

Departments of Pediatrics and Internal Medicine University of Wisconsin Medical School

12/91-2/95 Assistant Professor Department of Medical Microbiology and Immunology

University of Wisconsin Medical School

2/95-2/2000 Associate Professor Departments of Pediatrics, Internal Medicine, and Medical Microbiology and Immunology


2/2000 - Present Professor Departments of Pediatrics, Internal Medicine, and Medical Microbiology and Immunology


2007 Gerard B. Odell Professor of Pediatrics (Univ. of Wisconsin WARF Named Professorship)

2008 Director, Wisconsin Center for Infectious Disease

2008 Director, Microbes in Health and Disease NIH T32 Training Program

CERTIFICATION:

1979 Diplomate, American Board of Medical Examiners

1981 Written Boards in Pediatrics

1992 Complete Boards in Pediatrics


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HONORS AND AWARDS:

1973 Phi Beta Kappa Boston University, College of Liberal Arts

1978 Alpha Omega Alpha Boston University School of Medicine

1978 First Annual Louis Weinstein Prize for Excellence in Infectious Diseases Boston University School of Medicine

1986-88 Dalldorf Fellow in Medical Mycology

Infectious Diseases Society of America

1986 Young Investigator Award, American Society of Microbiology

1994 First Annual Gerard B. Odell Award for

Research Excellence, University of Wisconsin

1996 - 2003 Inaugural Burroughs Wellcome Scholar in Molecular Mycology

2002 Pfizer Annual Visiting Professor, Infectious Diseases

2002 Fellow, American Academy of Microbiology

2005 NIH MERIT Award

2006 -2007 Listed in “Best Doctors in America”

2007-2008 Listed in “Best Doctors in America”



2006 Elected to Association of American Physicians

2007 Inaugural recipient: Hartwell Foundation Scholar

2008 UW-Madison WARF Named Professorship (Gerard B. Odell Professor)


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2008 Sandler Foundation Senior Investigator Award in Asthma Research

2009 Elected to fellowship: (AAAS) American Association for Advancement in Science

2010 Elected to American Pediatric Society (APS)

2011 Elected Chairman, NIH PTHE Study Section 2010-2012

2011 Elected Chairman (2014) and Vice Chair (2012) Inaugural Gordon Research Conference on Immunology of Fungal Diseases

2011 Hilldale Award in the Biological Sciences

(UW-Madison award to a faculty member for distinguished achievements in the biological sciences)

SERVICE & COMMITTEES

NATIONAL 1995 Burroughs Wellcome Fund

Scientific Advisor on Molecular Mycology Research

1995 Institute of Medicine National Academy of Sciences Scientific advisor on vaccine development against medically important fungi

1994-present Ad Hoc member on Study Sections:

Bacteriology and Mycology SS2 (BM2) NICHD Training grants National Institutes of Health

1999-2001 Chair/Chair-Elect of Mycology Division American Society of Microbiology

2000-2005 NIH, NIAID Study Section BM-2, Regular Member

2005-present NIH (NIAID) Study Section Ad Hoc member – PTHE, and Vaccine Study Sections


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2009 NIH (NIAID) Study Section PTHE, Regular

Member

2005 Consultant, California Health Care Foundation Vaccine initiative for prevention of Coccidioidomycosis

2005 ASM Colloquium Working Group on

Environmental Microbial Pathogens

2007 ASM Colloquium Working Group on “The Fungal Kingdom” Diverse and Essential Roles in the Earth’s Ecosystem

2005-2006 Chair and Organizer: First ASM Sponsored

International Meeting on the Endemic Dimorphic Fungi, Denver CO 3/06

2006 Chair, Dimorphic Fungal Genome Consortium

2007 Chair, Nominating committee ASM Division F Chair (Medical Mycology)

2008 Nominating Committee for ASM Division F Chair

2010 Consultant, NovaDigm Vaccine Development against systemic fungal infections

2010 -2012 Chairman, NIH Study Section - PTHE

(Pathogenic Eukaryotes)

UNIVERSITY/MEDICAL SCHOOL 1994-1997 Scientific Advisory Board

UW Romis Program Project: Molecular Immunology of STD caused by Chlamydia

1984-1994 Infection Control Committee University of Wisconsin Hospital and Clinics

1996-2000 UW-Madison, Member, Biological Safety Committee


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1997-2000 UW-Madison, Biological Sciences Division

Executive Committee for Tenure and Promotions Vice Chair (1998-1999) Chair (1999-2000)

1999-present UW Medical School NIH K30 Training Grant Institutional Advisory Board

2001 UW Mycology Cluster Hire Advisory Committee

2001-2006 UW Medical School MSTP (MD/PhD) Program Member – Admission Committee

2005 University-Wide Committee for Revision of Tenure Guidelines

2005 University Search Committee, Medical School Dean

2006-2007 University Committee (The Executive Committee of the University of Wisconsin Faculty)

2006-2007 Medical School Retreat Advisory Committee

2006-2007 Deans Medical School Tenure Guideline Task Force

2006-2007 Board of Directors of PROFS, Inc. (University Public Representation Organization of the Faculty Senate)

2006-2007 UW-Madison Committee on Committees

2006-2007 UW-Madison Committee on Financial Emergencies

2006 Chair, Advisory Board, Committee on the formation of a UW-Madison Center for Public Health Microbiology and Infectious Disease

2008 Chair, Wisconsin Immunology Symposium

2008 Member, Chair of Medicine Search/Screen Committee


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2009 Member, University Provost Search/Screen Committee

2009 Member, Scientific Review/Selection Committee for Wisconsin Institute for Discovery

2009 Chair, Nominating Committee for University

Honorary Doctorate for Dr. June Kwon Chung

2010 University Task Force to Review UW Madison Graduate School Research Enterprise (Declined)

2010-2011 SMPH Research Transformation Implementation Committee

DEPARTMENT 1997-2006 Director, Pediatric Research Fellowship Training

1999-present Director, Division of Pediatric Infectious Diseases

2000-2006 Director, Pediatric Core Research Laboratories

2002-2006 Vice Chair for Research, Dept. of Pediatrics

2007 - present Research Advisory Committee in Pediatrics

2000-present Pediatrics Faculty Search Committees Genetics Division (member) Hematology and Oncology (member) Rheumatology Division (member) Medical Microbiology: Immunology (member) Chair, Search for Interim Chair, Pediatrics

1998-present Faculty Mentoring Committees

Jim Gern, M.D., Pediatrics (Member) Marlow Eldridge, M.D., Pediatrics (Chair) Joe Dillard, Ph.D., Microbiology (Member) Chris Sorenson, Ph.D., Pediatrics (Member) Dave Andes, M.D., Medicine (Member) Cheryl Sanchez, M.D., Pediatrics (Member) Istvan Danko, M.D., Ph.D., Pediatrics (Member) Loren Denlinger M.D., Ph.D. Medicine (Member) Rob Striker, M.D., Ph.D., Medicine (Member) Sunny Divat, M.D. Pediatrics (Chair) Dave Allen, M.D., Pediatrics (Chair)


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Judith Smith, M.D., Ph.D., Pediatrics (Chair) Elizabeth Cox, M.D., Ph.D, Pediatrics (Chair) Rob Striker, M.D., Ph.D. Medicine (Chair) Daniel Jackson M.D. Caitlin Pepperell (Chair)

EDITORIAL BOARDS

Infection and Immunity – 1990 - 2005 Microbial Pathogenesis “Faculty of 1000” Medical Mycology – Associate Editor (declined) PLoS pathogens – Associate Editor Cellular Microbiology Frontiers in Mycology BMC Immunology

REVIEWER

Science Nature Journal of Immunology Journal of Biological Chemistry New England Journal of Medicine Journal of Clinical Investigation Molecular Microbiology Cellular Microbiology Infection and Immunity Journal of Infectious Diseases Reviews of Infectious Disease Journal of Immunological Methods Antimicrobial Agents and Chemotherapy Medical Mycology Journal of Leukocyte Biology PLoS pathogens Cell: Cell Host and Microbe

SOCIETY MEMBERSHIPS

Infectious Disease Society of America American Society of Microbiology American Association for Advancement of Science Association of American Physicians (AAP) American Pediatrics Society


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TRAINING & MENTORING

Trainer in Ph.D. programs: Biotechnology Program MD/PhD (MSTP) Program (Member, Admissions Committee) Cellular and Molecular Pathology Program Microbiology Doctoral Training Program Molecular Biosciences Training Program Microbial Pathogenesis and Host Response Training Program Cellular and Molecular Biology Training Program

Ph.D. Thesis Advisor (Major Professor) Seon Kim (Microbiology Training Program; Completed) Peggy Rooney (Microbiology Training Program; Completed) Yong Zhang (Cellular and Molecular Pathology; Completed) Jessica Owens (Microbiology Training Program; Transferred) Julie Nemecek (Microbiology Training Program; Completed) Christopher Aaron Frye (Cellular and Molecular Pathology; Completed) Karen Ke (Cellular and Molecular Pathology; Completed) Brad Tebbets (Microbiology Training Program; Completed) Nicole Cooney (Microbiology Training Program; Completed) Current PhD and MD/PhD students (Major Professor) René Roy (MSTP program; Current) Jenna Lorenzini (CMB program; Current) Alana Sterkel (MDTP program; Current) Stephanie Lawry (CMP program; Current) Student PhD Thesis Committees Allison Scott (Woods lab; Completed 1999) Steve Gilles (Czuprynski lab; Completed 1998) Linnette Ortiz (DeMars lab; Completed 1997) Andreas Vasquez (Balish lab; Completed 1996) Jessica Jones (Balish lab; Completed 1996) Lisa Schopf (Mansfield lab; Completed 1994) Regan Theiler (Compton lab; Completed 2001)) Kimber Fischer (Woods lab; Completed 1999) John Luecke (Woods lab; Completed 2001) Michelle Timmerman (Woods lab; Completed 2001) Tinsley Davis (Mansfield lab; Completed) Dmitrios Pappagianis (Keller lab; Completed) Casey Scott Weathers (Paulnock lab; Completed) Julia Ng (Woods lab; Completed) Annete Stewart (Mansfield lab; Transferred) Erica Maldanado (Woods lab; Completed)


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Steven Gilles (Czuprynski lab; Completed) Meghan Bohse (Woods lab) Kendall Galbraith (Woods lab) Tajie Harris (Paulnock lab) A. Singh (Suresh Lab) Levi Yant (Watkins Lab) JunChao Cai (Burlingham lab) Sara Caldwell (Barclay lab) Kathy Monroe (Knoll lab) Philip Mudd (Watkins lab) Juan Paublo Giraldo Vela (Watkins lab) Ben Burwitz (O’connor lab) Crystal Tobin (Knoll lab) Saore Amaike (Keller lab) Graeme Garvey (Keller lab) Current (Ph.D. or M.D./Ph.D. candidates) Elizabeth Heath Heckman (McFalls Ngai lab) Matt Mead (Hull lab) Emilia Kruzel (Hull lab) Taylor Starnes (Huttenlocher lab) Kelsea Jewell (Keller lab) Beth Harvie (Huttenlocher lab) Tony Koehn (Sondel lab) Heather Taff (Andes lab) Mike Kahn (Smith lab)

Undergraduate Student Honorsʼ Thesis (Advisor) Dan Ruge (Completed 1998) Kristin Knudson (Completed 2000) Matt Noordsij-Jones (Completed 2000) Melanie Lo (Completed 2001; Undergraduate Hilldale Scholar) James Durbin (Completed 2001) Colin Kealey (Completed 2002; Undergraduate Hilldale Scholar) Sergio Gallardo (Undergraduate Hilldale Scholar) Current undergraduate advisees Douglas Stewart, Brandon Kelly, Cherish Westin, Becca Gollman

Postdoctoral Trainees Laura Hogan, Ph.D. (Completed 1997; funded by NIH NRSA; ICTR Staff Scientist) Wun-Ling Chang, M.D. (Completed 1997; funded by IDSA and NIH KO8; Professor) Mason Zhang, Ph.D. (Completed 1996; Professor) Tristan Brandhorst, Ph.D. (Completed; funded by American Lung Assoc/Parker B. Francis Foundation; Associate Scientist)


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Marcel Wuethrich, Ph.D. (Completed; funded by Swiss National Science Foundation and Morris Foundation; Senior Scientist) Theerapong Krajaejun, M.D. (Completed; funded by Ellison Medical Foundation; Assistant Professor) Phil Fisette, Ph.D. (Completed 2006; Industry) Richard Stein, M.D., Ph.D. (Completed 2006; Postdoc, NYU) Erika Heninger, Ph.D. (Completed 2009; Scientist) Current postdoctoral trainees Greg Gauthier, M.D. (Current; funded by IDSA, as Pfizer Scholar and NIH KO8) John Carmen, Ph.D. (Current; funded by NIH T32, and Hartwell Fellowship) Hugo Paes, M.D. (Current; funded by Sandler foundation) Som Nanjappa, Ph.D. (Current; funded by NIH RO1)

PUBLICATIONS PEER-REVIEWED ARTICLES (in chronological order beginning with most recent):

Roy R, Wuethrich M, Klein BS. Chitin elicits CCL2 from airway epithelial cells and induces CCR2 dependent innate allergic inflammation in the lung. J Immunology. In press. 2012

Tebbets B, Stewart D, Lawry S, Nett J, Nantel A, Andes D, Klein B. Identification and characterization of antifungal compounds using a yeast reporter bioassay. PLoS One. In Press. 2012.

Nanjappa S, Heninger E, Wüthrich M, Klein BS. Tc17 Cells Mediate Vaccine Immunity against Lethal Fungal Pneumonia in Immune Deficient Hosts lacking CD4+ T Cells. In Revision. PLoS Pathogens. 2012

Roy R, Klein BS. Dendritic cells in anti-fungal immunity and vaccine design. Cell Host and Microbe. In press. 2012.

Ersland K, Wuethrich M, Sullivan TS, Klein BS. Fungi subvert vaccine T cell priming at the respiratory mucosa by preventing chemokine induced influx of inflammatory monocytes. Immunity 2012; In press (April issue).

Nanjappa S, Henninger E, Wuethrich M, Klein BS. Protective antifungal memory CD8+ T cells are maintained in the absence of CD4+ T cell help and cognate antigen in mice. J Clin Inves. 2012;122:987-99.

Wuethrich M, Pick-Jacobs J, Gern B, Ke K, Frye CA, Sullivan T, Brennan MB, Filutowicz H, Obrien K, Schultz-Cherry S, Klein BS. Limited model antigen expression by transgenic fungi induces disparate fates during differentiation of adoptively transferred TCR Tg CD4+ T cells: robust activation and proliferation with weak effector function during recall. Infect Immu. 2012, 80:787-97.

Hung C, Gonzalez A, Wuethrich M, Klein B, Cole G. A protective vaccine against


Page 12Coccidioides regulates pulmonary inflammation by activation of IL-17+ and regulatory Foxp3+ T cells. Infect Immun 2011, 79:4511.

Wuethrich M, Gern B, Pick-Jacobs J, Sullivan T, Galles KJ, Filutowicz H, and Klein B. A T-cell receptor transgenic mouse reactive with multiple member of the family of systemic dimorphic fungi. J Immunol 2011; 187:1421-31.

Wüthrich M, Kraejun T, Legendre A, Klein BS. Safety and immunogenicity of a live attenuated vaccine strain of Blastomyces dermatitidis. Clinical and Vaccine Immunology 2011; 18:783-9.

Wuethrich M, Gern B, Pick-Jacobs J, Cooney N, Ersland K, Klein BS. Vaccine induced protection against three systemic mycoses endemic to North America requires T17 cells. J Clin Invest 2011; 121:554–568. [Epub ahead of print. (Selected as “Hot paper” and excerpted in Nature Immunology Reviews).

Rocco N, Carmen J, Klein BS. Blastomyces dermatitidis yeast inhibit nitric oxide production by the alveolar macrophage inducible nitric oxide synthase. Infect Immun 2011; 79:2385-95.

Pfister JR, Archer JR, Hersil S, et al. A large non-rural point source outbreak of blastomycosis near a yard materials collection site. Clinical Medicine and Research 2011. 9:57-65. Oct. 25th [Epub ahead of print].

Ersland K, Wuethrich M, Klein BS. Dynamic interplay among monocyte-derived, dermal and resident lymph node dendritic cells during the generation of vaccine immunity to fungi. Cell Host & Microbe 2010: 7; 474-87.

Gauthier GM, Sullivan, Galliardo S, Brandhorst TT, Klein BS. Regulation of iron metabolism and morphogenesis of yeast to mold by the GATA transcription factor SREB in Blastomyces dermatitidis. PLoS Pathogens 2010, 6: 1-16.

Poulsen M, Cafaro MJ, Erhardt DP, Little A, Gerardo NM, Tebbets B, Klein BS, Currie CR. Variation in Pseudonocardia antibiotic defense helps govern parasite- induced morbidity in Acromyrmex leaf-cutting ants. Environ Microbiol. In press. 2010. Epub ahead of print 11/18/2009.

Krajaejun T, Gauthier GM, Wuethrich M, Sullivan TD, Klein B. The Blastomyces dermatitis yeast phase specific gene bys1 regulates morphogenesis, but not virulence. Infect Immun 2010: 78; 2522-8.

Meece J, Anderson J, Klein B, et al.. Genetic diversity in Blastomyces dermatitidis: implications for PCR detection in clinical and environmental samples. Medical Mycol 2009: 48; 285-90.

Cooney N, Klein B. Adaptation to environmental stress in pathogenic fungi. Curr Opin Microbiol 2008,11:511-6.

Krajaejun T, Gauthier GM, Rappleye CA, Sullivan TD, Klein BS. The

4/9/12 Bruce Klein, MDPage 13

development and application of a GFP sentinel system for the identification of RNA interference in Blastomyces dermatitidis illuminates the role of septin in morphogenesis and sporulation. Eukaryotic Cell 6:1299, 2007.

Singh A, Wüthrich M, Klein B, Suresh M. Regulation of CD4+ T cell responses by TNF Receptors in an acute viral infection. J Virol 81:6502, 2007.

Gauthier GM, Andes D, Klein BS. Blastomycosis in solid organ transplant recipients: a report of 12 cases and review of the literature. Transplant Infect Dis Epub April 11 (ahead of print) 2007.

Wuethrich M, Fillutowicz H, Allen H, Deepe GS, Klein BS. Vb1+Jb1.1+/Va2+Ja49+CD4+ T cells that mediate resistance against infection with Blastomyces dermatitidis. Infect Immun 75: 193, 2007.

Wuethrich M, Fisette P, Filutowicz H, Warner T, Klein BS. CD40 co-stimulation differentially regulates CD4 and CD8 T-cell immunity to fungi. J Immunol 176:5538 2006.

Nemecek J, Wuethrich M, Klein BS. Global Control of dimorphism and virulence in fungi. Science 312:583, 2006.

Segal BH, Kwon-Chung J, Walsh TJ, Klein BS, Battiwalla M Holland S, Romani L. Immunotherapy for fungal infections. Clin Infect Dis 42;507, 2006.

Wuethrich M, Warner T, Klein BS. CD28 is required for optimal induction, but not maintenance, of vaccine induced immunity to Blastomyces dermatitidis. Infect Immun 73:7436-41, 2005.

Brandhorst T, Gauthier G, Klein BS. Calcium binding by the essential virulence factor BAD-1 of Blastomyces dermatitidis. J Biol Chem 280:42156-63. 2005.

Wüthrich M, Warner T, Klein BS. IL-12 is required for induction but not maintenance of protective, memory responses to Blastomyces dermatitidis: Implications for vaccine development in immune-deficient hosts. J Immunol 175:5288-97. 2005.

Wuethrich M, Filutowicz H, Brandhorst T, Finkel-Jimenez B, Klein BS. Additional evidence for non-adhesive pathogenic mechanisms of BAD1 on Blastomcyes dermatitidis. Med Mycol 44:41-9, 2005.

Rooney P, Klein BS. Identification and analysis of upstream sequence elements that activate transcription of the yeast-phase specific virulence gene BAD1 of Blastomyces dermatitidis. Eukaryotic Cell 3:785-94; 2004.

Brandhorst TT, Wuethrich M, Finkel-Jimenez B, Warner T, Klein BS. Exploiting type 3 complement receptors for TNF-α suppression, immune evasion, and progressive pulmonary fungal infection. J Immunol 2004; 173:7444-53.


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Wuethrich M, Filutowicz H, Warner T, Deepe GS, Klein BS. Vaccine induced immunity mediated against pathogenic fungi by CD8 T-cells in hosts with acquired and congenital absence of CD4 T-cells: a model for vaccine application in immune suppressed hosts. J Exp Med 197:1405, 2003.

Brandhorst T, Wuethrich M, Finkel-Jimenez B, and Klein BS. A C-terminal EGF domain governs BAD1 localization to the yeast surface and fungal adherence to phagocytes, but is dispensable for deviation of host immunity and pathogenicity of Blastomyces dermatitidis. Mol Microbiol 48: 53-65, 2003.

Wuethrich M, Filutowicz H, Warner T, Klein BS. Requisite elements in vaccine immunity to Blastomyces dermatitidis: plasticity uncovers vaccine potential in immune-deficient hosts. J Immunol. 169:6969, 2002.

Sullivan T., Rooney P., Klein BS. Agrobacterium tumefaciens transfers T-DNA into single chromosomal sites of dimorphic fungi and yields homokaryotic progeny from multinucleate yeast. Eukaryotic Cell. 1:895, 2002.

Finkel-Jimenez B, Wuethrich M, Klein BS. BAD1, an essential virulence factor of Blastomyces dermatitidis suppresses host TNF-α production through TGF-β dependent mechanisms. J Immunol 168:5746-5755, 2002.

Rooney P, Sullivan T, Klein BS. Selective expression of the virulence factor BAD1 upon morphogenesis to the pathogenic yeast form of Blastomyces dermatitidis: evidence for transcriptional regulation by a conserved mechanism. Mol Microbiol 39: 875-889, 2001.

Finkel-Jimenez B. E., Wüthrich M. Brandhorst T., Klein BS. Down-regulation of phagocyte TNF-α production by the WI-1 adhesin imparts pathogenicity on Blastomyces dermatitidis. J Immunol 166:2665-73, 2001.

Zhang M, Brandhorst T, Kozel T, Klein BS. The role of yeast cell surface constituents β-glucan and WI-1 glycoprotein in regulating activation and binding of complement by Blastomyces dermatitidis. Infect Immun 69:7559-64, 2001.

Proctor M.E., Klein B.S., Jones J.M., Davis J.P. Cluster of pulmonary blastomycosis in a rural community: Evidence for multiple high-risk environmental foci following a sustained period of diminished precipitation. Mycopathologia 153:113-120, 2001.

Wuethrich M, Fillutowicz H, Klein B. Mutation of the WI-1 gene yields an attenuated Blastomyces dermatitidis strain that induces host resistance. J Clin Invest 106:1381-89, 2000.

Wüthrich M., Finkel-Jimenez B. E., Klein B.S. Il-12 as an adjuvant to WI-1 adhesin immunization augments delayed type hypersensitivity, shifts the subclass distribution of IgG antibodies, and enhances protective immunity to Blastomyces dermatitidis infection. Infect Immun 68:7172-74, 2000.


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Brandhorst T, Klein BS. Cell wall biogenesis of Blastomyces dermatitidis: Evidence for a novel mechanism of cell surface localization of a virulence associated adhesin via extracellular release and reassociation with cell wall chitin. J Biol Chem 275 (11):7925-7934, 2000.

Wuethrich M, Klein BS. Anti-WI-1 antibodies that enhance experimental pulmonary infection with Blastomyces dermatitidis. J Infect Dis 181 (5):1720-1728. 2000.

Chang WL, Audet RA, Aizenstein BA, Hogan LH, Klein BS. T-cell epitopes and HLA restricting elements of an immunodominant antigen of Blastomyces dermatitidis. Infect Immun 68:502-510. 2000.

Klein BS, Richard Squires R, Lloyd J, Legendre AM. Canine antibody responses to Blastomyces dermatitidis WI-1 antigen in 122 dogs with blastomycosis. Am J Vet Res 61(5):554-558, 2000.

Brandhorst T, Wuethrich M, Warner T, Klein BS. Targeted gene disruption reveals and adhesin indispensable for pathogenicity of Blastomyces dermatitidis. J Exp Med 189; 1207-1216. 1999. (Featured on the Cover of April 19 Issue).

Guiles S, Klein BS, Czuprynski C. Effect of canine macrophages on adherence and growth of Blastomyces dermatitidis: evidence for a soluble factor that enhances growth. 1999. Microbial Pathogenesis 27:395-405.

Chang WL, van der Heyde H, Klein BS. Flow Cytometric Quantitation of Yeast. A Novel Technique for use in Animal Model work and in vitro Immunologic Assays. J Immunol Meth 211; 51-63. 1998.

Wuethrich M, Chang WL, Klein BS. Immunogenicity and Protective Efficacy of the WI-1 adhesin of Blastomyces dermatitidis. Infect Immun 11; 5443-49. 1998.

Audet RA, Brandhorst TA, Klein BS. Purification in quantitiy of the secreted form of WI-1, a major adhesin of Blastomyces dermatitidis yeasts. Protein Express & Purific 11: 219-226, 1997

Hogan LH, Klein BS. Transforming DNA integrates at multiple sites in the dimorphic fungal pathogen Blastomyces dermatitidis. Gene 186: 219-226, 1997.

Klein BS, Aizenstein BA, Hogan LH. African strains of Blastomyces dermatitidis that lack the major surface adhesin WI-1. Infect Immun 65:1505-1509, 1997.

Zhang XP, Klein BS. Mechanisms of initiation, binding, and processing of complement component C3 by Blastomyces dermatitidis yeast. Infect Immun 65:1849-1855, 1997.

Gern JE, Dick EC, Kelly EA, Vrtis R, Klein BS. Rhinovirus-specific T-cells recognize both shared and serotype-restricted viral epitopes. J Infect Dis 175:1108-1114, 1997.


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Hogan LH, Klein BS, Levitz S. Virulence factors of medically important fungi. Clin Microbiol Rev 9:469-488, 1996.

Klein BS and Newman SL. Role of Cell Surface molecules on Blastomyces dermatitidis in host-pathogen interactions. Trends in Microbiology 4:246-251,1996.

Hogan LH, Josvai S, Klein BS. Genomic cloning, characterization, and functional analysis of the major surface adhesin WI-1 on Blastomyces dermatitidis yeasts. J Biol Chem 270:30725-30732; 1995.

Newman SL, Chaturvedi S, Klein BS. The WI-1 antigen on Blastomyces dermatitidis yeasts mediates binding to human macrophage CD18 and CD14 receptors. J Immunol 154:753-761, 1995.

Hogan LH, Klein BS. Altered expression of a-(1,3)-glucan on genetically related strains of Blastomyces dermatitidis that differ in virulence. Infect Immun 62:3543-3546, 1994.

Klein BS, Chaturvedi S, Hogan LH, Jones JM, Newman SL. Altered expression of the surface protein WI-1 in genetically related strains of Blastomyces dermatitidis that differ in virulence regulates recognition of yeasts by human macrophages. Infect Immun 62:3536-3542, 1994.

Klein BS, Jones JM. Purification and characterization of the major antigen WI-1 from Blastomyces dermatitidis, yeasts, and immunological comparison with A antigen. Infect Immun 62:3890-3900, 1994.

Soufleris AJ, Klein BS, Courtney BT, Proctor ME, Jones JM. The utility of anti-WI- 1 serologic testing in diagnosing blastomycosis in Wisconsin residents. Rev Infect Dis 19:87-92, 1994.

Klein BS, Hogan LH and Jones JM. Immunologic recognition of a 25-amino acid repeat arrayed in tandem on a major antigen of Blastomyces dermatitidis. J Clin Invest 92:330-337, 1993.

Klein BS, Sondel PM and Jones JM. WI-1, a novel 120kD surface protein on Blastomyces dermatitidis yeasts, is a target antigen of cell-mediated immunity in human blastomycosis. Infect Immun 60:4291-4300, 1992.

Klein BS and Jones JM. Isolation, purification and radio-labeling of a novel 120 Kd surface protein on Blastomyces dermatitidis yeasts to detect antibody in infected patients. J Clin Invest 85:152-161, 1990.

Fisch P, Malkovsky M, Kovats S, Sturm E, Braakman E, Klein B, Voss S, Morrissey L, DeMars R, Welch W, Bolhuis R, and Sondel P. Recognition by human Vg9/Vd2 T-cells of a GroEL homolog on Daudi Burkitt's lymphoma cells. Science 250:1269-1273, 1990.


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Klein BS, Bradsher RW, Vergeront JM and Davis JP. The development of long- term specific cellular immunity after acute Blastomyces dermatitidis infection: Assessments following a large point-source outbreak in Wisconsin. J Infect Dis 161:97-101 1990.

Klein BS, Vergeront JM, Kaufman L, Turner S, Kumar UN, Mathai G, Varkey B, and Davis JP. Serologic tests for blastomycosis: analysis of experience during a large point-source outbreak in Wisconsin. J Infect Dis 155:262-8, 1987. Klein BS, Vergeront JM, DiSalvo AF, Kaufman L and Davis JP. Two outbreaks of blastomycosis in Wisconsin: Isolation of Blastomyces dermatitidis from riverbank soil and evidence of its transmission along waterways. Am Rev Respir Dis 136:1333-8, 1987.

Klein BS, Vergeront JM, Weeks RJ, Kumar UN, Mathai G, Varkey B, Kaufman L, Bradsher RW, Stoebig JF, Davis JP, and the Investigation Team. Isolation of Blastomyces dermatitidis in soil associated with a large outbreak of blastomycosis in Wisconsin. N Engl J Med 314:529-34, 1986. (Lead article with accompanying Editorial: “Leave it to Beaver”)

Klein BS, Kuritsky JN, Chappell WA, Kaufman L, Green J, Davies SF, Williams JE and Sarosi GA. Comparison of the enzyme immunoassay, immunodiffusion, and complement fixation tests in detecting antibody in human sera to the A antigen of Blastomyces dermatitidis. Am Rev Respir Dis 133:144-8, 1986.

Klein BS, Vergeront JM, Davis JP. Epidemiologic aspects of blastomycosis, the enigmatic systemic mycosis. Sem Respir Infect 1:29-39, 1986.

Klein BS and Davis JP. A laboratory-based surveillance of human blastomycosis in Wisconsin between 1973 and 1982. Am J Epidemiol 122:987-903, 1985.

Klein BS, Perloff WH, and Maki DG. Protective isolation reduces nosocomial infection during pediatric intensive care: A prospective, randomized study. N Engl J Med 320:1714-21, 1989.

Maki DG, Klein BS, McCormick RD, Hassemer CA, Alvarado CA, Zilz MA, and Liegel AR. Nosocomial Pseudomonas Picketti bacteremias traced to narcotic tampering and contamination. A case for selective drug screening of health care personnel. JAMA 265:981-86, 1991.

Klein BS, Vergeront JM, Blaser MJ, Edmonds P, Brenner DJ, Janssen D and Davis JP. An outbreak of gastroenteritis due to Campylobacter jejuni and thermotolerant Campylobacter fetus subsp. fetus associated with raw milk. JAMA 255:361-4,1986.

Klein BS, Jaeger D, Davis JP, Schell WL, Gary GW and Yolken RH. Serodiagnosis of rotavirus during an epidemic of gastroenteritis on an adult


Page 18orthopedic unit. Serodiag Immunother 1:33-44, 1987.

Klein BS, Rogers MF, Patrician LA, White ME, Burgdorfer W, Schell WL, Kochel RL, Marchette NJ, McPherson JT, Nelson DB, Yolken RH, Wortmann D, and Davis JP. Kawasaki syndrome: A controlled study of an outbreak in Wisconsin. Am J Epidemiol124:306-16, 1986.

Klein BS, Michaels JA, Rytel MW, Berg KG and Davis JP. Nosocomial hepatitis A. A multinursery outbreak in Wisconsin. JAMA 252:2716-21, 1984. Klein BS, Dollete FR and Yolken RH. The role of respiratory syncytial virus and other viral pathogens in acute otitis media. J Pediatr 101:16-20, 1982.

SUBMITTED ARTICLES

Brandhorst T, Roy R, Wüethrich M, Tonelli M, McCaslin D, Klein B. Structure and function of a fungal adhesin that binds heparin and mimics thrombospondin-1 by blocking T cell activation. Submitted.

Tebbets B, Yu Z, Stewart D, Zhao L, Jiang Y, Xu L, Shen S, Klein BS. Identification of antifungal natural products by yeast bioassay: insights into macrotetrolide drug spectrum, potency and mode of action. Submitted

Durkin M, Hage C, Connolly P, Macedo de Oliveira A, Klein BS, Wheat L. Detection of antibodies to Blastomyces dermatitidis cloned surface protein BAD-1 by enzyme immunoassay. Submitted.

IN PREPARATION

Carmen J, Sullivan T, Lorenzini J, Klein B. Calcium channel signaling components Cch1 and Myd1 and morphogenesis of Blastomyces dermatitidis.

Carmen J, Klein B. Influence of soil amoeba on morphogenesis of mold spores to yeast in systemic dimorphic fungi.

Roy R, Wuethrich M, Klein B. Complement components C3a and C5a in chitin induced allergic asthma due to Aspergillus fumigatus.

Roy R, Wuethrich M, Blackwell T, Yuell F, Klein B. Obligate role of respiratory epithelial cells during chitin induced allergic asthma due to Aspergillus fumigatus.

CHAPTERS/REVIEWS/PROCEEDINGS:

Klein BS. Blastomycosis. Textbook of Pediatric Infectious Disease. 5th Edition. Feigin and Cherry. 2012.

Klein BS. Role of dendritic cells in immunity to fungi. Cell Host and Microbe.


Invited review. 2012.

Wuethrich M, Deepe G, Klein BS. Adaptive immunity to fungi. Annual Reviews of Immunology. Invited review. 2011.

Gauthier G, Klein BS. Systemic Mycoses of North America. Clin Micro Rev. Invited review. 2011.

Wuethrich M, Klein BS. T-helper subsets and adaptive immunity to fungi. Current Trends in Immunology. Invited review. 2011.

Gauthier GM, Klein BS. Blastomycosis. In Nelson’s Textbook of Pediatrics. 2009 edition.

Gauthier GM, Klein BS. How fungi sense their environment, adapt and become pathogenic in mammalian hosts. Microbe. September 2008.

Klein BS, Tebbets B. Dimorphism and virulence in Fungi. Current Opinions in Microbiology. 2007 Aug 22; [Epub ahead of print].

Cutler J, Deepe GS, Klein BS. Advances in combating fungal diseases: vaccines on the threshold. Nature Rev Microbiol 5: 13-28, 2007

Nemecek JN, Wuethrich M, Klein BS. Two-component signal transduction in pathogenic fungi. Methods in Enzymology. 422:465-87. 2007

Klein BS. Molecular approaches to fungal vaccine development. In Molecular pathogenesis of fungi. Heitman J, Filler, Edwards J, Mitchell A. Eds. ASM press. Washington, DC. 2006.

Wuethrich M., Klein BS. Innate and adaptive immunity to Blastomyces dermatitidis. In Fungal immunology from an organ perspective. Eds, Fidel P, Huffnagle G. Plenum publishers. 2006.

Deepe GS, Wuethrich M, Klein BS. Progress in vaccination for histoplasmosis and blastomycosis: coping with cellular immunity. Medical Mycology 43:381-9, 2006.


Page 20

DiSalvo A, Klein BS. Blastomycosis. Topley and Wilson Microbiology & Microbial Infections, 10th ed. 2004.

Klein B.S. (Invited Guest Editor). Host-microbe interactions: Fungi. Curr Opin Microbiol 6:325, 2003.

Rooney P.J., Klein B.S. Linking fungal morphogenesis and virulence. Cellular Microbiology 4: 127-137, 2002. (Featured on Journal Cover).

Brandhorst T. Tristan, Rooney P.J., Sullivan T., Klein B.S. Genetic manipulation of Blastomyces dermatitidis uncovers pathogenic mechanisms. Trends Microbiol 10:25-30, 2002

Brandhorst T. Tristan, Rooney P.J., Sullivan T., Klein B.S. Molecular biology of the pathogenic fungus Blastomyces dermatitidis. Int J Med Microbiol. 292; 363-371, 2002.

Klein BS, Chang WL. Pathogenesis of Blastomycosis. Ed. Calderone R. In Pathogenesis of Fungal Diseases. Marcel Deker. January 2002.

Klein BS. Turning up the heat on Histoplasma capsulatum. Perspectives. Science 290 (5495): 1311, 2000. (November 17 issue).

Klein BS. Adhesins in the pathogenesis of blastomycosis. Ed. Casadevall A. In Current Opinions in Microbiology. Section entitled “Host-microbe interactions: Fungi. Current Biology Publications. Curr Opin Microbiol 3: 339-343, 2000.

Kwon-Chung KJ, Goldman WE, Klein BS, Szaniszlo PJ. Fate of Transforming DNA in pathogenic fungi. Fate of transforming DNA in pathogenic fungi. Medical Mycology 36 Suppl 1:38-44, 1998.

Dixon DM, Casadevall, Klein BS, Mendoza L, Travassos L, Deepe GS. Development of vaccines and their use in the prevention of fungal infections. Medical Mycology. 36 Suppl 1:57-67, 1998.

Deepe GS, Klein BS. Blastomyces dermatitidis. In Infectious Diseases 2nd edition, ed. by S.L. Gorbach, J.G. Bartlett, and N.R. Blacklow. W.B. Saunders, Philadelphia. 1998.

Klein BS, Hogan LH, Newman SL. Surface molecules on Blastomyces dermatitidis, and their role in virulence. American Society of Microbiology News 63:140-145, 1997.

Klein BS, Chaturvedi S, Newman SL. Interactions of Blastomyces dermatitidis with human monocyte derived macrophages: The WI-1 antigen mediates binding to CD18 and CD14 receptors. In Proceedings of the 5th International Symposium on Topics in Mycology. Host-Fungus Interplay. Stanford, CA. June 1997.


Page 21Chang WL, Klein BS. Blastomycosis. In Principles of Medical Mycology. Deepe G, Mitchell T, Hazen K, Cutler J, (eds). ASM publication. In press.

Klein BS. Immunobiology and pathogenesis of blastomycosis. In Sarosi G, ed. Blastomycosis: The First Century. Seminars in Respiratory Infections. 12; 198-205,1997.

Hogan LH, Klein BS. Cloning and characterization of the gene sequence corresponding to the 25 amino acid repeat of Blastomyces dermatitidis WI-1 surface antigen. In Molecular Biology of Pathogenic Fungi. Maresca B, Kobayaski GS, (eds). Telos Press. 1994.

Klein BS and Chesney PJ. Blastomycosis. In Nelson JD (ed.), Current therapy of pediatric infectious disease. 3rd edition, St. Louis: The C. V. Mosby Company. 1993.

Klein BS. Immunology of blastomycosis. In Al-Doory Y, DiSalvo AF (eds.). Blastomycosis. Plenum Publishing Corporation, 1992.

Klein BS. Treatment of blastomycosis. In Rakel RE (ed.). Conn's Current Therapy. 1991 edition. W.B. Saunders Company.

Klein BS. Blastomycosis. In Jacobs PH, Nall L (eds.). Antifungal drug therapy: A complete guide for the dermatologist. Marcel Dekker, Inc. 1990.

Klein BS, Blastomycosis. In Nelson JD (ed.). Current therapy of pediatric infectious disease. 2nd edition, St. Louis: The C.V. Mosby Company. 1988.

Klein BS. Formulary section. In Biller JA, Yeager AM (eds.) Harriet Lane Handbook: A Manual for Pediatric Houseofficers, 9th edition. Chicago: Yearbook Medical Publishers,1981.

LETTERS:

Klein BS, Vergeront JM, Davis JP and Weeks RJ. Diagnosis of blastomycosis. N Engl J Med 315:763, 1986.

Klein BS, Perloff WH and Maki DG. Protective isolation during pediatric intensive care. Letter. N Engl J Med 322:553, 1990.

RECENT INVITED NATIONAL AND INTERNATIONAL PRESENTATIONS FEBS Advance Lecture Course in Human Fungal Pathogens. Molecular Pathogenesis of Blastomycosis. Nice France. May 2013. Gordon Conference. Vice Chair and Co-organizer. Galvaston, TX. January 2013.

Genetics Society of America. Invited Speaker. Host: Fungal Pathogen Interactions. Madison Wisconsin. August 2012.


Page 22

ISHAM meeting. Berlin Germany. Invited Speaker. Vaccination against fungi. June 2012. (Declined)

University of Montana at Bozeman. Invited Seminar. Regulation of vaccine immunity to fungi. Department of Microbiology. April 2012.

University of Rochester. Invited Seminar. Vaccine Immunity to Fungi. Department of

Pediatrics Research Seminar Series. March 2012.

UCLA. Invited Seminar. Regulation of vaccine immunity to fungi. Department of Microbiology. March 2012.

Keystone Conference. Drug discovery in parasites and fungi. Sante Fe, NM. Invited Speaker. January 2012.

Midwest Society for Pediatric Research. Keynote address. Through the glass Drk-ly: what fungi can teach us. October 2011.

Midwest Neglected Infectious Diseases. Invited speaker. Notre Dame. August 2011.

Immunity to fungi.

FEBS Advance Lecture Course in Human Fungal Pathogens. Molecular Pathogenesis of Blastomycosis. Nice France. May 2011. (Declined)

Sandler Foundation Annual Meeting. The search for the chitin receptor. San Francisco, CA. May 2011.

Gordon Conference. Regulation of vaccine immunity to dimorphic fungi. Galvaston, TX.

January 2011

Mt. Sinai School of Medicine. Invited Seminar. Departments of Microbiology and Pediatrics. December 2010.

Texas A&M University. Department of Microbiology Seminar Series. Host:fungal pathogen interactions. November 2010.

Annual Society of Veterinary Medicine. Vaccine development against Blastomycosis. Annaheim, CA. June 2010.

Sandler Foundation Annual Meeting. The search for the chitin receptor. San Francisco, CA. May 2010.

ASM Conference on Dimorphic Fungi. Innate and adaptive immunity to fungi.

Miami, FL. March 2010. National Institutes of Health. Novel targets for new anti-fungal drug development.

Bethesda, MD. March 2010.


Page 23Department of Microbiology and Immunology. Invited Speaker. University of Texas

Health Sciences Center. April, 2010.

Hartwell Foundation. Invited Speaker. Anti-fungal new drug development. Memphis, TN. October 2009.

General Meeting of the American Society for Microbiology. Anti-fungal Immunity. Philadelphia, PA. May 2009.

Sandler Foundation. Annual Meeting of Senior Investigators. Role of chitin in Asthma. San Francisco, CA. May 2009

International Society of Human and Animal Mycology. Invited Speaker. Regulation of phase transition in dimorphic fungi. Tokyo Japan. May 2009.

U Mass Worcester. Department of Microbiology and Medicine. Invited Speaker.

Innate immunity to fungi. April 2009.

University of Cincinnati. Department of Pediatrics. Invited Seminar Speaker. Selective recognition and immunity to fungi. April 2009.

Hartwell Foundation Symposium. Invited Speaker. Regulation of morphogenesis

and virulence in fungi. Memphis, TN. October 2008. Midwest Meeting on Microbial Pathogenesis. Invited Speaker. Selective recognition

and immunity to fungi. Madison, Wisconsin. September 2008. Woods Hole Course in Molecular Pathogenic Mycology. Invited Seminar Speaker.

What Blastomcyes dermatitidis can teach us about pathogenesis of fungal diseases. August 2008.

Gordon Conference on fungi. Session Chair. Plymouth NH. June/July 2008.

U Mass Medical School. Regulation of morphogenesis and virulence in fungi. Department of Medicine and Microbiology. April 2008.

University of Tennessee. Virulence mechanisms of pathogenic fungi. Invited Seminar.

Department of Microbiology. March 2008.

Ohio State University. Immunology and Pathogenesis of Fungal Diseases. Invited Seminar. Departments of Microbiology and Divisions of Infectious Disease. December 2007.

American Academy of Microbiology Colloquium. Environmental and pathogenic fungi.

November 2007. Tuscon, Arizona.

Cold Spring Harbor Meeting in Microbial Pathogenesis and Host Immune Response. Invited Speaker: “Genetic Regulation of Virulence in Fungi.” New York, September 2007.


Page 24

Snowmass, Colorado. FASEB conference in Microbial Pathogenesis. July 2007. Invited Faculty Participant. Dimorphism and virulence in fungi.

Woods Hole Course in Molecular Pathogenic Mycology. Invited Seminar Speaker. What Blastomcyes dermatitidis can teach us about pathogenesis of fungal diseases. August 2007.

Annual meeting of the American Society of Microbiology. Invited Seminar Speaker.

Blastomcyes dermatitidis illuminates pathogenesis of fungal diseases. May 2007.

International FEBS Course on Fungal Pathogens. Invited Seminar Speaker. Environmental Sensing in Fungi. Nice France, May 2007.

Georgetown University. Invited Seminar Speaker. Department of Microbiology. Spring 2007.

Asilomar Fungal Meeting. Invited seminar speaker. Environmental sensing in pathogenic fungi. March 2007.

University of Vermont. Two component signaling in pathogenic fungi. Invited Seminar

Speaker. Burlington, Vermont. Department of Microbiology. January 2007.

Fort Dodge Animal Health. Prospects for vaccination against pathogenic fungi. Invited Seminar Speaker. Fort Dodge, Iowa. September 2006.

International ISHAM Mycology meeting. T-cell priming and memory immunity to fungi. Paris, France. June 2006.

UW-Madison Campus Wide Immunology Retreat. Invited Symposium Speaker. Madison, WI. May 2006.

Annual General Meeting of ASM. Invited Symposium Speaker. Environmental

sensing and morphogenesis and virulence of fungi. Orlando, Florida. May 2006.

International ASM meeting on Dimorphic Fungal Pathogens. Invited Speaker. APC:T-

cell interactions in systemic fungal infections. Denver, CO. March 13-17. 2006. International FEBS Course on Fungal Pathogens. Invited Seminar Speaker. Use

of model antigens for probing mechanisms of adaptive immunity to Fungi. Nice, France, May 2005

Washington University at St. Louis. Physician-scientist training program in

Infectious diseases. Invited Seminar Speaker. Molecular mechanisms of fungal pathogenesis. November 2004.

Interscience Conference on Antimicrobial Agents and Chemotherapy. Invited

Seminar Speaker. Novel approaches to vaccine immunity to fungi. Washington, D. C. October 2004.


Page 25 Burroughs Wellcome Fund. Pathogenesis of Infectious Diseases. Invited Expert Panel

Member. August, 2004. Clearwater, Florida.

University of Minnesota. Department of Pediatrics. Research Seminar. Medical mycology for pediatricians in the new millennium. May 2004.

Annual Meeting of the American Society for Microbiology. Invited Speaker at Mycology

Symposium. Molecular bases of fungal pathogenicity. New Orleans, LA. May 2004.

NIH sponsored workshop on “Animal Models of Invasive Aspergillosis: Critical Needs and Future Directions”. Invited Panel Member. Bethesda, Maryland on May 21, 2004.

Johns Hopkins School of Medicine. Departments of Medicine and Microbiology. Invited Guest Speaker at Microbiology and Immunology Seminar Series. Linking fungal morphogenesis, pathogenicity, and evasion of host immunity. April 2004.

2004 Harold C. Neu Infectious Diseases Conference sponsored by IDSA. Invited

Guest Speaker. Molecular pathogenesis of fungal infections. April 29 – May 2, 2004. Loews Ventana Canyon Resort, Tucson, Arizona

Focus on Fungal infections. National Meeting sponsored by Pfizer. Invited Speaker.

Approaches to vaccine prevention of blastomycosis. March 2004. New Orleans, LA (declined).

American Academy of Microbiology, Blue ribbon panel. Colloquium entitled “From Outside to Inside: Environmental Microorganisms as Human Pathogens” Portland, Oregon. February 2004.

University of Minnesota. Department of Microbiology. Invited Speaker for

Departmental Seminar. Linking fungal morphogenesis, pathogenicity, and evasion of host immunity. December 2003.

Geneva, Switzerland. Foundation Merieux. Invited speaker. International

Symposium: Immunological approaches to nosocomial fungal infections. November 2003 (declined).

Cold Spring Harbor Conference on Mechanisms of Microbial Pathogenesis. Invited

Speaker. Role of fungal cell surfaces constituents in pathogenesis of infection. September 2003.

International Society for Human and Veterinary Mycology. Invited speaker. Session

on Molecular biology of fungi. Pathogenic mechanisms of Blastomyces dermatitidis. San Antonio, TX. May 2003.

International Society for Human and Veterinary Mycology. Invited speaker. Session

on new developments in the endemic mycoses. Application of genetic tools to


Page 26study pathogenicity of Blastomyces dermatitidis. San Antonio, TX. May 2003.

International Society for Human and Veterinary Mycology. Session organizer

and speaker. Session on Vaccine Immunity. Vaccine immunity to Blastomyces dermatitidis. San Antonio, TX. May 2003.

Annual Meeting of Wisconsin Thoracic Society/American Lung Association of Wisconsin.

State of the Art Lecture. Investigations of Pulmonary Blastomycosis: Bedside to Bench. April 2003.

Pfizer Visiting Professor in Infectious Disease. Invited Guest. Medical University of South Carolina. Charleston, SC. November 2002.

International Conference on Human Pathogenic Fungi. Invited Speaker. Development of genetic tools to study the pathogenicity of Blastomcyes dermatitidis. Sao Paulo, Brazil. June 22-24 2002.

Interscience conference on antimicrobial agents and chemotherapy. Invited Speaker.

Basic science session on vaccine development in fungi. San Diego, CA. September 2002.

International Conference on Microbiology. Invited speaker. Development of genetic

tools to study the pathogenicity of Blastomcyes dermatitidis. Paris, France. July 2002.

International Conference on Paracoccidiodes Braziliensis. Invited Speaker. Development of genetic tools to study pathogenesis of Blastomcyes dermatitidis. Sao Paulo, Brazil. June 25-28 2002.

Gordon Conference. Biology of Host-Pathogen Interactions. Invited Participant

and Speaker. Host-cell attachment, invasion, and modification: Blastomyces dermatitidis. Newport, RI, June 2002.

American Society for Microbiology. Developmental Biology of Microbial Pathogens.

“Role of morphogenesis in virulence of systemic dimorphic fungi.” Salt Lake City, Utah. May 2002.

Boston University School of Medicine. Immunology Training Program Seminar.

Vaccine induced immunity to intracellular pathogens. April 2002. University of Massachusetts Medical School. Microbiology Seminar Series. Linking

fungal morphogenesis, adherence, and evasion of host immunity. March 2002. University of Iowa Medical School. MSTP Seminar Series. Vaccine induced immunity

to intracellular pathogens. Insights from a recombinant fungal vaccine. Spring 2002.

Albert Einstein College of Medicine. Departments of Medicine and Microbiology and

Immunology. "Linkage of morphogenesis, immune evasion, and virulence in


Page 27the dimorphic fungus Blastomyces dermatitidis". Invited Speaker in Seminar Series. September 2001.

American Society for Microbiology. Annual Meeting. Symposium on Host Immune

responses. Mechanisms of vaccine immunity in response to a genetically- engineered live, attenuated strain of Blastomyces dermatitidis. Orlando, Florida. May 2001.

National Institutes of Health. Division of Molecular Microbiology. Seminar Series.

Molecular and cellular determinants of the host: pathogen interaction in blastomycosis. May 2001.

Washington University at St. Louis. Department of Molecular Microbiology Seminar.

Molecular and cellular determinants of host: pathogen interaction in blastomycosis. April 2001.

University of Cincinnati. Department of Pediatrics Research Seminar Series. A

phase- regulated fungal gene linked with virulence. January 2001. University of Oklahoma Health Science Center. Microbiology and Immunology Seminar

Series. Molecular and cellular determinants of host: pathogen interaction in blastomycosis. December 2000.

Snowmass, Colorado. Biannual FASEB conference in Microbial Pathogenesis. August 2000. Invited Faculty Participant. Genetic analysis of virulence in Blastomyces dermatitidis.

Columbia University. College of Physicians and Surgeons. Dept of Microbiology

and Immunology. Microbial Pathogenesis Seminar Series. Pathogenesis of Blastomycosis. April 2000

Woods Hole: Annual Course in Molecular Mycology. Invited Faculty Lecturer. August

1999. Genetic approaches to analysis of pathogenicity of Blastomyces dermatitidis.

University of Cincinnati. Department of Pathology: Lecture Series in Microbial Pathogenesis. April 1999. Genetic approaches to analysis of pathogenicity of Blastomyces dermatitidis.

Asilomar Fungal Genetics and Molecular Biology Meeting. Asilomar CA. 1999.

Invited Speaker at Symposium entitled. Advances in Genetic Manipulation of Medical Fungi.

American Society of Microbiology. Annual Meeting. Chicago, IL. 1999. Invited Speaker

at Symposium entitled. “Progress in Understanding Virulence of Medically Important Fungi.”

The Genetic Society of America. Yeast Genetics and Molecular Biology Meeting.

Washington, DC. 1998. Invited Speaker at Session entitled "Molecular Genetics of Pathogenic Yeasts.”


Page 28

Medical College of Ohio. 1998. NIH Workshop. Vaccine development for medical fungi. Invited Speaker in Workshop. Prospects for vaccination against Blastomyces dermatitidis.

Duke University Division of Microbiology and Immunology. 1998 Raleigh, NC. Invited

Lecturer for course on Microbial Pathogenesis and to Divisional Seminar. Immunobiology and Pathogenesis of blastomycosis.

International Society of Human and Veterinary Mycology. 1997. Parma, Italy. Invited

Speaker at Session entitled "Molecular Genetics of Pathogenic Fungi."

International Society of Human and Veterinary Mycology. 1997. Parma, Italy. Invited Speaker at Session entitled "Vaccine Strategies against the Pathogenic Fungi."

The Genetic Society of America. Annual Yeast Genetics and Molecular Biology Meeting. 1996. Madison, WI. Invited Speaker. Workshop "Molecular Genetics of Pathogenic Yeasts."

International Union of Microbiological Societies. 8th International Congress of Mycology Division. 1996. Jerusalem, Israel. Invited Speaker at Session entitled “Virulence Factors of Human and Animal Pathogens.” (session cancelled)

Annual Meeting of the American Society of Microbiology. 1996. New Orleans, LA.

Invited Speaker at Mycology Symposium. Lecture entitled “Modulation of host:pathogen interactions in blastomycosis by the surface adhesin WI-1 on Blastomyces dermatitidis.”

Annual Meeting of the Medical Mycology Society of America. 1996. New Orleans, LA.

Invited Speaker at Symposium on Fungal Vaccine Development. Lecture entitled "Contemporary approaches toward vaccine development against Blastomycosis."

5th International Symposium on Topics in Mycology entitled “Host-Fungus Interplay.” 1995. Stanford University, San Jose, CA. Invited Speaker. Lecture entitled “Role of the surface protein WI-1 in adherence of Blastomyces dermatitidis to human macrophages.”

National Institutes of Health Workshop on Fungal Antigens, Immunity, and Vaccine

development. 1995. Big Sky, Montana. Invited Speaker. Lecture entitled: “Blastomyces dermatitidis: Native and recombinant peptide immunoreactivity.”

CURRENT GRANT SUPPORT:

ACTIVE


Page 29

1/04-1/14 NIH R37 AI-35681 (MERIT Award) Title: "Molecular pathogenesis of blastomycosis" P.I.: Bruce S. Klein, M.D.; 15% effort Annual direct support, $300,000 Goal: Investigate role of BAD1 adhesin structure, function and regulation in pathogenesis of blastomycosis

7/07-6/12 NIH RO1 AI-40996

Title: "Mechanisms of anti-fungal vaccine immunity" P.I.: Bruce S. Klein, M.D.; 15% effort Annual direct support, $300,000 Goal: To elucidate the mechanisms of vaccine induced protective immunity to a pathogenic fungus

10/09 – 9/12 NIH RC1 Challenge Grant

Title: Targeting Hybrid Histidine Kinase for Broad Spectrum Anti-fungal Therapy PI: Klein, BS (10% effort) Direct Support: $500,000/year Summary: Multidisciplinary group that seeks to identify new antibiotics from small molecules.

9/10 – 8/13 NIH RC4 Challenge Grant

Title: Discovery of natural product-based drugs from bacterial symbionts of insects PI: Currie, C. Role: Co-investigator (5% effort) Direct support: $750,000/year Summary: Multidisciplinary group that seeks to identify new antibiotics from microbes on insects.

7/11 – 6/16 NIH RO1 AI-093553

Title: Regulation of vaccine induced anti-fungal T17 cells PI: Wuethrich, Co-investigator-Klein(10%effort) Annual Direct: $300,000 Summary: This grant address vaccine induced anti-fungal immunity mediated by Th17 cells.

7/08 – 6/13 NIH T32 Training Grant Title: Microbes in Health&Disease Training Grant PI (and Director): Bruce Klein, M.D. (8% effort)


Page 30Direct support: $451,000 per year Summary: This is a competing renewal of an NIH Training Grant that supported pre-doctoral training in microbial pathogenesis. The renewal proposes support for training of both pre- and postdoctoral PhD and MD trainees interested in the beneficial and harmful roles of Microbes.

7/08 – 7/12 Sandler Program for Asthma Research

Title: The Chitin Receptor and its Role in Asthma Pathogenesis PI: Klein, BS (10% effort) Total Direct Costs: $750,000 ($ 250,000/year) Project Summary: This work is designed to discover novel receptors for chitin and elucidate their role in promoting allergic inflammation and asthma in animal models of disease.

07/07 – 06/12 University of Wisconsin MERC Collaborative

Health Sciences Program Title: Wisconsin Infectious Disease Drug Discovery PI: Klein, BS Total direct costs: $300,000 ($100,000/year) Summary: The goal is a multidisciplinary project that involves screening of small molecules and novel synthetic compounds to discover new drugs against selected fungal, bacterial and viral pathogens of human hosts

7/08 – 7/12 University of Wisconsin MERC Collaborative Health Sciences Program

Title: Wisconsin Center for Infectious Disease (WisCID) PI: Klein, BS (20% effort) Total direct costs: $1,511,306 Summary: The goal of this project is to establish a campus Center for Infectious Disease with research and training goals to be administratively housed at the Microbial Sciences Building.

7/11 – 7/13 WARF Accelerator Program Title: Anti-fungal drug discovery PI: Klein, BS


Total direct costs: $100,000 Summary: Continued work on drug discovery

7/10 – 7/13 NIH F30 Training Fellowship

(PI: Roy, R; Mentor: Klein) Role of chitin in the pathogenesis of asthma Direct Costs: $180,000 Goal: To provide training in research on asthma during graduate studies of an MSTP student.

7/10 – 6/12 Hartwell Foundation Fellowship (PI: Carmen, J; Mentor, Klein, B.) Role of calcium signaling in fungal morphogenesis Direct costs: $100,000 Fellowship training for John Carmen, PhD.

PENDING

9/12-8/17 NIH RO1 AI-40996 Title: "Mechanisms of anti-fungal vaccine immunity" P.I.: Bruce S. Klein, M.D.; 20% effort Annual direct support: $380,000 Goal: To elucidate the mechanisms of vaccine induced protective immunity to a pathogenic fungus

7/01/11- 6/30/13 American Lung Association

Fellowship Training Grant (Paes, PI; Klein, Mentor) Topic: Fungal Dimorphism

7/01/11- 6/30/13 American Heart Association

Fellowship Training Grant (Paes, PI; Klein, Mentor) Topic: Fungal Dimorphism


Page 32

COMPLETED IN LAST 7 YEARS

4/04 – 3/10 NIH PO1 AI-0161298 Title: Defending against systemic Mycoses This is a Mycology Research Unit Program Project involving individual RO1’s from 4 PI’s at 3 institutions. PI: George Deepe, University of Cincinnati Project #2 PI: Bruce Klein; 10% effort Anti-fungal memory immunity. Direct Support: $600,000/yr (project 2; $125,000/yr)

05/07 – 4/10 Hartwell Foundation Research Scholar Award

Title: Stemming the growing tide of systemic fungal infections in children PI: Klein, BS (2.5% effort) Total direct costs: $300,000 ($100,000/year) Project summary: The goal is to analyze how dimorphic sense environmental stress, signal morphogenesis in response to it, and develop inhibitors of this sensing system, which is a key trigger in fungal pathogenesis.

12/07 – 11/09 NIH/NIAID 1 R21

Priming of Antifungal T-Cells at Mucosal and Systemic Sites PI: M. Wuethrich; Co-PI: BS. Klein (5% effort) Direct costs: $275, 000 Project summary: This project investigates the mechanisms by which 2 routes of vaccination trigger CD4+ T cells during the priming and the recall phases. We will create a TCR transgenic mouse and establish an adoptive transfer system to delineate activation T cells in these two immunologically divergent settings.

7/02 - 6/08 NIH RO1 AI-50882

Title: Comparative and functional genomics of Histoplasma and Blastomyces This is a consortium of 2 institutions proposing a comparative/functional genomic analysis of H. capsulatum & B. dermatitidis. PI: Bill Goldman. Co-PI B. Klein; 20% effort Direct Support: $500,000/year


Page 33

7/04 – 7/06 Infectious Disease Society of America Fellowship Pfizer Scholar in Medical Mycology Regulation of dimorphism in Blastomyces dermatitidis Fellowship for Greg Gauthier, M.D. Sponsor: Bruce Klein, M.D. Direct support: $80, 000

11/03 – 11/06 Ellison Foundation Foundation Fellowship in International Nutrition Immune evasion strategies of Blastomcyes dermatitids Fellowship for Theerapong Krajaejun, M.D. Sponsor: Bruce Klein, M.D. Direct support: $80, 000

7/05-7/06 MACC Fund: Midwest Athletes for Curing Cancer (Klein, PI) Pilot Research Project on Multi-host Pathogenicity of Aspergillus flavus Direct Support: $40, 000

7/96-6/03 Burroughs Wellcome Fund

Scholar in Molecular Mycology "Genetic Immunization against Pathogenic Fungi" Goal: This 5-yr award is to develop a paradigm for naked DNA vaccination against pathogenic fungi using B. dermatitidis as a model. PI: Bruce Klein, M.D.; 10% effort Direct Support: $400,000

1/97-12/02 NIH KO8 Mentored Clinical Scientist Award "CD4 T-cell Regulation of Immunity in Blastomycosis" Goal: 5-yr.Training grant for Dr. W.L. Chang (PI) Sponsor: Bruce Klein, M.D. Direct Support: $431,500

7/06 – 7/12 NIH KO8 PI: Gauthier, Greg. Sponsor: Bruce Klein, M.D. Mentored Clinician Scientist Training Grant Regulation of morphogenesis in dimorphic fungi Training Grant for Greg Gauthier, M.D. Direct support: $500,000


Page 34

7/00 – 6/02 American Lung Association “Regulation of phase-specific virulence genes in systemic dimorphic fungi” Recipient: Bruce Klein, M.D. Direct Support: $35,000/yr

7/01 – 7/04 Parker B. Francis Pulmonary Fellowship

Genetic bases of Aspergillus pathogenicity in multiple vertebrate and invertebrate hosts.

Fellowship for Tristan Brandhorst, Ph.D. Sponsor: Bruce Klein, M.D. Direct support: $120,000

8/00-9/02 Morris Animal Foundation Live attenuated vaccine against canine blastomycosis Fellowship for Marcel Wuethrich, Ph.D. Sponsor: Bruce Klein, M.D. Direct Support: $35,000/year

7/02 - 7/03 Industrial &Economic Development Research UW-Madison/UIR Program Title: Prevention/diagnosis of blastomycosis This is a technology transfer grant to commercialize products for a new company BlastoVac, Inc., based on technology licensed from inventions patented in our lab. Direct support: $50,000

PAST GRANT SUPPORT

7/86 – 6/88 Gilbert Dalldorf Scholar in Medical Mycology “Immune responses to Blastomyces dermatitidis” Goal: To identify major antigens of the fungus PI: Bruce Klein, 100% effort Annual direct support: $35,000

7/88 – 6/93 Physician Scientist Award (K11 award) "Antifungal role and action of human cytolytic lymphocytes” Goal: To elucidate T-cell mechanisms of protective immunity to Blastomyces dermatitidis P.I.: Bruce S. Klein, M.D.; 75% effort Annual direct support: $50,000


Page 35

7/91 – 6/96 First Award (R29 award) “Antifungal role & action of human T-cell subsets” Goal: To elucidate T-cell mechanisms of protective immunity to Blastomyces dermatitidis P.I.: Bruce S. Klein, M.D.; 75% effort Annual direct support: $75,000

5/95-5/2000 NIH RCDA (KO4 award) AI 01308 “Molecular pathogenesis of blastomycosis” Goal: Research Career Development Award P.I.: Bruce S. Klein, M.D.; 80% effort Annual direct support: $67,000

PATENTS: 1. Method of isolation of a 120 kD cell wall protein antigen from Blastomyces dermatitidis yeasts, and use of the antigen to detect specific antibody and lymphocyte responses and to immunize animals. (Approved).

2. Cloning, sequencing and expression of a major antigen of Blastomyces dermatitidis. (Approved). 3. A recombinant attenuated vaccine strain of Blastomyces dermatitidis. (Approved). 4. A global regulator of dimorphism and virulence in fungi. (Approved). 5. Compounds that act on hybrid histidine kinase and exert anti-fungal activity (Pending).

6. Novel methods of purification of BAD-1 from Blastomyces dermatitidis. (Pending)

Documents

CURRICULUM VITAE PERSONAL DATA · CURRICULUM VITAE PERSONAL DATA Name: Bruce S. Klein, M.D. Office Address: Division of Infectious Diseases . Center for Health Sciences, Room K4/434