Leon Oosterik May 2015 1

Leon Oosterik

Personalia Address: Kanaaldijk 1

7471 SG Goor Date of birth: 09-03-1987 Mobile: +31 630 79 69 12 Gender: M Nationality: Dutch E-mail address: leon.oosterik@gmail.com Driving license: B (Dutch)

Work experience 2011 - 2014 PhD in Bioscience Engineering Leuven/Brussels (BEL) KU Leuven and CODA-CERVA Organizing practical immunology courses for Master’s students

Education 2011 - 2014 PhD in Bioscience Engineering Leuven/Brussels (BEL)

KU Leuven and CODA-CERVA The potential of disinfectants and bacteriophages to reduce avian colibacillosis Promotor: prof. Bruno Goddeeris (KU Leuven) Co-promotor: prof. Patrick Butaye (CODA-CERVA) Scholarship: • Feb 2011 - Jan 2013: Federal Public Service

Health, Food Chain Safety and Environment • Feb 2013 - Jul 2014: CODA-CERVA

Date of graduation: Oct 2014 2008 - 2010 Master in Animal Sciences Wageningen (NLD)

Specialisation: Animal Health and Behaviour Wageningen Universiteit Major thesis: Natural antibodies as parameters for mastitis resistance in dairy cows Minor thesis: Development of a chikungunya subunit vaccine; Exchange of the native signal sequence of E1 and E2 glycoproteins for the Honeybee Melittin signal sequence

Internship: Detection of Escherichia coli infection Edmonton (CAN) of the liver in newly hatched chicks by entering the navel University of Alberta

Leon Oosterik May 2015 2

2005 - 2008 Bachelor in Animal Sciences Wageningen (NLD) Minor in biology of infectious diseases of humans and animals

Wageningen Universiteit Bachelor thesis: Culture of avian embryos in semi-shell-less and shell-less culture

Technical skills

Antibiotic/disinfectant susceptibility testing, bacterial/phage isolation, bacterial transformation, biofilm formation assay, cloning, DNA purification, gel electrophoresis, immunohistochemistry, infection studies, ligation, PCR, phage amplification, restriction enzyme digestion, sequencing

Extra courses

Laboratory Animal Science (Article 9 certified)

Languages Dutch: native English: good German: basic

IT/computer skills

General: Microsoft Word, Excel and PowerPoint Specialized: FastPCR, Chromas, CLC Sequence Viewer

Extracurricular activities 2006 - 2008 Secretary student swimming association 'Aquifer' Wageningen (NLD)

Hobby’s

Mountain biking, running, swimming, travelling

References References upon request

Leon Oosterik May 2015 3

Publications Tsonos*, J., Oosterik*, L.H., Tuntufye, H.N., Klumpp, J., Butaye, P., De Greve, H., Hernalsteens, J.- P., Lavigne, R., Goddeeris, B.M. 2014. A cocktail of in vitro efficient phages is not a guarantee for in vivo therapeutic results against avian colibacillosis. Veterinary Microbiology 171 (3-4), 470-479. * shared first authorship Oosterik, L.H., Peeters, L., Mutuku, I., Goddeeris, B.M., Butaye, P. 2014. Susceptibility of avian pathogenic Escherichia coli from laying hens in Belgium to antibiotics and disinfectants and integron prevalence. Avian Diseases 58 (2), 271-278. Oosterik, L.H., Tuntufye, H.N., Butaye, P., Goddeeris, B.M. 2014. Effect of serogroup, surface material and disinfectant on biofilm formation by avian pathogenic Escherichia coli. The Veterinary Journal 202, 561-565.

Presentations Oosterik, L.H., Peeters, L., Butaye, P. Susceptibility of avian pathogenic Escherichia coli from laying hens in Belgium to antibiotics and disinfectants and integron prevalence. National Symposium on Applied Biological Sciences. Gembloux, Belgium. September 07, 2014. Poster. Tsonos*, J., Oosterik*, L.H., Tuntufye, H.N., Butaye, P., De Greve, H., Hernalsteens, J-P, Lavigne, R., Goddeeris, B.M. A cocktail of in vitro efficient phages is not a guarantee for in vivo therapeutic results against avian colibacillosis. Belgian Society for Microbiology Annual Symposium. Brussels, Belgium. November 26-27, 2013. Poster Tsonos*, J., Oosterik*, L.H., Tuntufye, H.N., De Greve, H., Lavigne, R., Hernalsteens, J-P, Goddeeris, B.M. Treatment of experimentally infected chickens with a bacteriophage cocktail. 10th International Veterinary Immunology Symposium. Milan, Italy. Augustus 28-September 01, 2013. Poster Tsonos, J., Oosterik, L.H. , Klumpp, J., Willems, J., Goddeeris, B.M., De Greve, H., Lavigne, R., Hernalsteens , J-P. Bacteriophages as an alternative way to treat and prevent APEC infections. Viruses of Microbes. Brussels, Belgium. July 16-20, 2012. Poster * shared first authorship

Conferences Escherichia coli and the Mucosal Immune System: Interaction, Modulation and Vaccination. Ghent, Belgium. July 2-5, 2011.