34
R.B. Shrestha, Page 1 January 26, 2014 1 Ram B. Shrestha, Ph.D. Postdoctoral Research Associate 13 Insectary Building, Department of Entomology, Iowa State Universities, Ames, Iowa 50011, Phone: 515-294-6542, Fax: 515-294-7406 Cell Phone: 806-549-6285, E-mail: [email protected] Web: http://www.ent.iastate.edu/dept/faculty/gassmann/people/ram-shrestha Publications: http://scholar.google.com/citations?user=JphkwaMAAAAJ&hl=en Career Goal: My immediate career goal is to get an academic university positions that provides an opportunity to use my knowledge, skills and passion for research, teaching and extension in the field of arthropod biology, ecology, and pest management. My long-term goal is to become a successful teacher, extension specialist and research scientist and develop internationally recognized research and teaching program in collaboration with national and international scientists from various institutions working toward common interest of improving human knowledge and skills on arthropod ecology and help develop products, plans, policies, tools and technologies that directly benefit growers and agriculture clientele at large. Academic Qualifications: Ph.D. Biological Science (Entomology concentration), Texas Tech University, Department of Biological Sciences, 2011. Dissertation Title: Ontogenic Morphometry and Genetic Diversity of Lygus hesperus (Hemiptera: Miridae) (GPA: 3.97). M.S. Entomology, Texas Tech University, Department of Plant and Soil Science, 2004. Thesis Title: Ecology of Arthropods in Texas High Plains Cotton and Development of Sampling Plans for Selected Cotton Pests (GPA: 4.0). B.Sc. Agriculture (Plant Protection Major), Institute of Agriculture and Animal Sciences, Rampur, Chitwan, Nepal, 1991. (Grade: First division). Employment History: June 2013-Current Postdoctoral Research Associate, Department of Entomology, Iowa State University, Ames, IA. Sept 2012-June 2013 Senior Research Associate, Cotton Entomology Program, Texas A&M, AgriLife Research, Lubbock, TX. Dec 2009-Aug 2012 Research Associate, Cotton Entomology Program, Texas A&M AgriLife Research, Lubbock, TX. Curriculum Vitae

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Page 1: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 1

January 26 2014

1

Ram B Shrestha PhD

Postdoctoral Research Associate

13 Insectary Building Department of Entomology

Iowa State Universities Ames Iowa 50011

Phone 515-294-6542 Fax 515-294-7406

Cell Phone 806-549-6285 E-mail shrestrbiastateedu

Web httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

Publications httpscholargooglecomcitationsuser=JphkwaMAAAAJamphl=en

Career Goal

My immediate career goal is to get an academic university positions that provides an opportunity

to use my knowledge skills and passion for research teaching and extension in the field of

arthropod biology ecology and pest management My long-term goal is to become a successful

teacher extension specialist and research scientist and develop internationally recognized

research and teaching program in collaboration with national and international scientists from

various institutions working toward common interest of improving human knowledge and skills

on arthropod ecology and help develop products plans policies tools and technologies that

directly benefit growers and agriculture clientele at large

Academic Qualifications

PhD Biological Science (Entomology concentration) Texas Tech University Department of

Biological Sciences 2011 Dissertation Title Ontogenic Morphometry and Genetic

Diversity of Lygus hesperus (Hemiptera Miridae) (GPA 397)

MS Entomology Texas Tech University Department of Plant and Soil Science 2004 Thesis

Title Ecology of Arthropods in Texas High Plains Cotton and Development of Sampling

Plans for Selected Cotton Pests (GPA 40)

BSc Agriculture (Plant Protection Major) Institute of Agriculture and Animal Sciences

Rampur Chitwan Nepal 1991 (Grade First division)

Employment History

June 2013-Current Postdoctoral Research Associate Department of Entomology Iowa State

University Ames IA

Sept 2012-June 2013 Senior Research Associate Cotton Entomology Program Texas AampM

AgriLife Research Lubbock TX

Dec 2009-Aug 2012 Research Associate Cotton Entomology Program Texas AampM AgriLife

Research Lubbock TX

Curriculum Vitae

RB Shrestha Page 2

January 26 2014

2

May 2002-Dec 2009 Graduate Research Assistant Cotton Entomology Program Texas AampM

AgriLife Research Lubbock TX

Jul 1994-May 2002 Plant Protection Officer Division of Agriculture Ministry of Agriculture

Kathmandu Nepal

Jan 1992-May 1994 Research Assistant Department of Plant Pathology Institute of Agriculture

and Animal Sciences Rampur Nepal

Immigration Status

Permanent Resident (authorized to work in the United States)

Summary of Scientific Publications (authoredco-authored see pp 16-26 for detailed)

Publication Category Number Published

Peer-reviewed journal articles 15

Refereed proceeding papers 2

Non-refereed proceeding papers 35

Project reports 24

Scientific abstracts 23

Papers in preparationinternal review 5

Career total 99 (published only)

Note My 15 peer reviewed journal papers has been cited 148 times from scientist

all over the world (from 17 different countries)

Summary of Scientific Presentations (see pp 26-35 for detailed list)

Presentation Category Number Presented

International invited oral 9

National invited oral 13

National submitted oral 33

International poster 8

National poster 36

Career total 99

RB Shrestha Page 3

January 26 2014

3

Teaching Student Advisory and Supervision at Texas Tech University

Teaching Involved in syllabus development lecturing in class and posting lecture slides and

other teaching material online in WebCT and Blackboard online server grading exams

and evaluating student performance

2011 ldquoIntroductory Entomologyrdquo undergraduate level online course fall semester

2011 ldquoAdvanced Insect Ecologyrdquo graduate level spring semester

2010 Invited guest lecturer for Integrated Pest management graduate level course spring

2009 ldquoAdvanced Insect Ecologyrdquo graduate level spring semester

Graduate Advisory Served in academic advisory committee of a graduate student (Angela

Fernando MS in Crop Protection Department of Plant and Soil Science Texas Tech

University) Thesis title ldquoOverwintering Biology of Lygus hesperus in the Texas High

Plainsrdquo

Student Supervised Provided technical guidance to MS level students in the program Trained

6 junior MS students in our laboratory Helped in experimental design data

collection and analysis in their MS thesis research projects Supervised 2

undergraduate research fellows and many summer student assistants

Informal Teaching gt400 in field one day-long growersrsquo training and demonstration sessions on

various aspects of crop protection in Nepal

Grants Honors Awards Fellowships and Scholarships (total $185624)

2013 Honor for outstanding leadershipndashplaque presented by Society of Overseas Nepalese

Entomologist (SONE) in annual business meeting of SONE November 11 2013

Austin Texas

2013 Honor in recognition of dedicated service- plaque presented by Cotton Entomology

Program Texas AampM AgriLife Research Center Lubbock TX in May 24 2013

2013 CO-PI Cotton Incorporated - Texas State Support Committee competitive grant

(Characterization of Cotton Crop Response to Thrips Injury for Improved Thrips

Management in Texas High Plains Cotton) $27000 per year (2012-2014 funded

annually)

2013 CO-PI Cotton Incorporated - Texas State Support Committee competitive grant

(Development of Economic Threshold and Management Recommendations for Lygus

in Texas High Plains Cotton) $30000 per year (2012-2014 funded annually)

2012 New career professional development (MONSANTO sponsored) travel assistantship

award by ESA ($424)

RB Shrestha Page 4

January 26 2014

4

2012 CO-PI Cotton Incorporated - Texas State Support Committee competitive grant

(Characterization of Cotton Crop Response to Thrips Injury for Improved Thrips

Management in Texas High Plains Cotton) $27000 per year (2012-2014 funded

annually)

2012 CO-PI Cotton Incorporated - Texas State Support Committee competitive grant

(Development of Economic Threshold and Management Recommendations for Lygus

in Texas High Plains Cotton) $30000 per year (2012-2014 funded annually)

2010 CO-PI International Cotton Research Center competitive grant (Lygus Bugs

Investigation of Predisposition Factors Causing Outbreaks in Order to Strengthen

Cotton Pest Management Strategies) $65000 (2010-2011)

2010 Travel grant from Biological Science Department and Graduate School TTU for

participation at 58th

annual meeting of ESA San Diego CA (Dec 12-15) ($800)

2009 Travel grant from Biological Science Department and Graduate School TTU for

participation and presentation at 57th

annual meeting of ESA Indianapolis IN (Dec

13-16) ($1000)

2009 Third place oral presentation award for student competition at Entomological Society

of America Southwestern Branch Meeting Stillwater OK (Feb 23-26)

2008 Travel grant from Biological Science Department and Graduate School for

participation at 56th

annual meeting of ESA Reno NV (Nov 16-19) ($1000)

2007 Travel grant from Biological Science Department and Graduate School TTU for

participation at 55th

annual meeting of ESA San Diego CA (Dec 9-12) ($700)

2006 Travel grant from Biological Science Department and Graduate School TTU for

participation at 54th

annual meeting of ESA Indianapolis IN (Dec 10-13) ($700)

2003 West Texas Agricultural Chemicals Institute ($1000 scholarship award)

2003 Leland Chandler Endowed Scholarship from Graduate School TTU ($500)

2003 Harold and Mary Dregne Graduate Scholarship from Graduate School TTU ($500)

2001 Netherlands Fellowship Program International Training Course on IPM in IAC

Wageningen the Netherlands (March 18-June 30)

2000 UNDP-IPM Training Fellowship International Training Course on crop protection in

CICAT Guangzhou China (May 5 to July 4)

1998 FAO IPM project in rice Trainers Training Fellowship Trainers Training on IPM in

rice in Dhanusa Nepal (April 6 to July 10)

1993 USAIDNepal Diploma in Biotechnology Training Fellowship Laboratory Training

on Plant Biotechnology in RLABB Kathmandu Nepal (Feb 13-May 13)

RB Shrestha Page 5

January 26 2014

5

Peer Review Service for Entomology Journals

I have reviewed many scientific papers for following entomological journals

Journal of Insect Science

Journal of Economic Entomology

Environmental Entomology

Neotropical Entomology

Entomologia Experimentalis et Applicata

Extension Experience in the USA

During my graduate programs (Master and PhD) from 2002 to 2011 I worked as graduate

research assistant in Texas AampM AgriLife Research and Extension Center Lubbock During this

period I got opportunity to interact closely with many extension specialists and research scientists

IPM and county agriculture extension agents growers crop consultants industry representatives

university professors and USDA scientists I have participated in many agricultural extension

activities Dr Jim Leser (Professor Extension Entomology in Texas AampM) was my masters

graduate advisory committee member I have closely worked with Dr David Kerns (Associate

Professor Extension Entomology in Texas AampM) in various collaborative extension projects

Below are the lists of some of my extension experience gained in Lubbock Texas

Participated in a Corn Rootworm Field day in Boon Iowa August 8 2013

Participated and given invited presentation in 3 crop consultant meetings in Texas

Participated and given invited talks in 4 plains cotton growers and cotton incorporated

annual meetings

Participated and given invited talk in 1 county extension agents refresher training

conferences

Participated in 5 field days and turn row meetings

Participated in 4 industries annual technology update meetings

Organized 4 insect expo insect zoo in science spectrum museum

Conducted many product evaluation field trials in growers field

Attended many commodity group (plains cotton growers advisory group) meetings

Given gt80 presentations for various audience in many meeting conferences

symposium workshops

Guided 3 group of international delegates and scientist visit or tours

Given 1 invited talk in research-extension interaction science conference

RB Shrestha Page 6

January 26 2014

6

Conducted many season-long insect pest sampling or scouting in various experimental

fields for many extension faculties collaborators

Conducted product evaluation field research in collaboration of various industries

Given 1 invited guest lectures for graduate and undergraduate classes in Texas Tech

University

Collaborated with many Extension specialists (Extension entomologists Irrigation

engineers Weed scientistAgronomist Pathologists Economists Plant breeders etc) on

various extension program and field research

Collaboration with many IPM agents county extension agents crop consultants USDA

scientists University professors and many graduate undergraduate students

Collaborated with many entomology faculties from various universities

Organized and moderated symposium in national and regional levels entomology

society meetings

Taught 3 graduate and undergraduate level entomology courses in university

Delivered entomology science and career demonstrations tour and presentations to

many groups of middle school students undergraduate students and upper bound

programrsquos students

Extension Experience in Nepal

I was a Crop Protection Extension Officer in the Department of Agriculture Nepal for 8 years

(1994-2002) I was the team leader of crop protection Extension programs in the district The goal

of my crop protection program was to disseminate modern crop protection technologies to

growers As a district IPM coordinator and crop protection officers I had managed district level

IPM program with the help of 15 junior field technicians and technical associates Examples of

extension activities conducted are as follows

Training and talks gt400 in-field training sessions at grower meetings

Field product evaluation and result demonstrations gt400 crop protection

demonstrations

Mass communications Conducted a radio talk program on rice blast disease and two

TV talk programs on pest management in mushroom cultivation in Nepal

Field days Organized four farmersrsquo field day

Farmersrsquo field schools Conducted three IPM farmersrsquo field schools in rice and cole

crops

Crop disease and insect pest diagnostic service

Pesticide regulation implementation Activity included pesticide registration resellers

training and pesticide inspection

Crop protection program planning monitoring and evaluation (regional and national)

RB Shrestha Page 7

January 26 2014

7

Postharvest loss management program such as grain storage improvement zero-energy

potato storage and cellar fruit storage

Training of junior agriculture technicians and progressive lead farmers

Distribution of posters leaflets booklets pamphlets and calendars

Crop protection training kits and subsidies distribution

Collaboration with research scientists in National Agriculture Research Council for

technology update and feedback on new crop protection technologies

Annual progress report publication (1995-2001)

Growers competition and award program

Collaborative Team Work Experiences

I have worked in close collaboration with several scientists and people from other national and

international laboratories and have published scientific papers coauthored with more than 30

people from different institutions

Leadership and Service to Professional Societies

Contributions to Professional Societies

2013 ESA Annual Meeting Member Symposium organizer Austin TX (November 10-13)

2013 ESA Annual Meeting Graduate Student Ten-Minute Paper competition session

moderator Austin TX (November 10-13) P-IE Section

Monday November 11 2013 800 AM-1200 PM

2012-2013 President Society of Overseas Nepalese Entomologists (SONE)

2012 ESA Annual Meeting Member Symposium organizer Knoxville TN (Nov 11-14)

2012 ESA Annual Meeting Symposium moderator Knoxville TN (November 11-14) P-IE

Section Environmental Entomology Symposium Tuesday November 13 2012 800

AM-1215 PM

2011-2012 Vice president Society of Overseas Nepalese Entomologists (SONE)

2011 ESA Annual Meeting Student Oral Competition Judge Reno NV (November 13-16)

2010 ESA Annual Meeting Symposium Organizer and Moderator San Diego CA

(December 12-16)

2010 ESA Annual Meeting Student Volunteer San Diego CA (December 12-16)

2009 ESA-SWB Annual Meeting Student Volunteer Stillwater OK (February 23-26)

2009 ESA-SWB Student Affairs Committee Member

2009 ESA Annual Meeting Student Volunteer Indianapolis IN (December 12-16)

RB Shrestha Page 8

January 26 2014

8

2009 ESA Annual Meeting Student Poster Competition Judge Indianapolis IN (December

12-16)

2008 ESA Annual Meeting Student Volunteer Reno NV (December 12-16)

2006 ESA-SWB Annual Meeting Student Volunteer Austin TX (February 28-March 2)

2006 ESA-SWB Annual Meeting Student Poster Competition Judge Austin TX (February

28-March 2)

2004 ESA-SWB Annual Meeting Student Volunteer Lubbock TX (February 23-26)

2004 ESA-SWB Annual Meeting Student Affairs Committee Member Lubbock TX

(February 23-26)

Conference Symposium Management

2014 Shrestha R B S R K Jakka and A J Gassmann 2014 co-organizing a symposium

(proposal accepted) in ESA NCB Annual Meeting Proposed title Advancements and

Challenges in Western Corn Rootworm Management Des Moines IA (March 9-10

2014)

2013 Shrestha R B B K Gautam J Rijal and S Gautam 2013 Jointly organized and

moderated Member symposium on ldquoPromoting Entomological Collaboration through

Inter-societal Network and Information Sharingrdquo for the 61th

annual meeting of ESA

Austin TX (November 10-13) Tuesday November 12 2013 600 PM-1000 PM

2012 Shrestha R B and R R Pandey 2012 Jointly organized and moderated Member

symposium on ldquoGlobal Collaboration for the Development of Next Generation IPM

Programrdquo for the 60th

annual meeting of ESA Knoxville TN (November 11-14)

Wednesday November 14 2012 800 AM-1200 PM

2010 Shrestha R B and M N Parajulee 2010 Jointly organized and moderated Member

symposium on ldquoMolecular and Other Novel Tools in Ecological Researchrdquo for the 58th

annual meeting of ESA San Diego CA (December 12-15) Wednesday December 15

2010 115 PM-330 PM

Active Memberships in Professional and Honor Societies

Member of Entomological Society of America

Member of Ecological Society of America

Member of Society of Southwestern Entomologists

Member of High Plains Association of Crop Consultants

President of Society of Overseas Nepalese Entomologists

Member of the National Scholars Honor Society

Member of Golden Key International Honor Society TTU Chapter

Member of Honor Society of Agriculture Gamma Sigma Delta TTU Chapter

RB Shrestha Page 9

January 26 2014

9

Member of American Association for Advancement in Science

Founder Member of Nepalese Student Association Texas Tech University

Professional Development Trainings and Workshops

In addition to my formal academic courses I have learned various important skills through

thirteen professional development trainings and workshops The training includes grant

writing training computer programing (R and Python) Integrated Pest Management (IPM)

training Training of Trainers (TOT) and plant biotechnology training Immuno-marking and

Enzyme Linked Immuno Sorbent (ELISA) training microsatellite DNA marker discovery

training teaching enhancement (Blackboard and WebCT) training laboratory safety (handling

radioactive materials pesticide application and shipment of hazardous materials) Compliance

training for conducting regulated Monsanto cotton field trial and training on cotton growth and

development monitoring using COTMAN software

I have also participated in short training courses in professional development such as time

management supervision project management problem solving conflict management scientific

methods information security awareness work place ethics creating discrimination free work

spaces and reporting fraud waste and abuse

Computational or Quantitative Skills and Experience

Through formal course work short training work experience and self-learning exercises I have

acquired computer skills on the application of 29 different computer software and programs

and used them in various research projects My computational skills include statistical data

analysis using SAS SPSS MATLAB SigmaPlot and ArcGIS online course preparation and

teaching using Blackboard and WebCT integrated reference management using EndNote

Medley and Reference Manager documentation database management and technical

communication using Microsoft Office Word Excel Access PowerPoint and Outlook

population genetics and phylogenetic data analysis using PAUP PHYLIP ARLEQUIN

STRUCTURE GenAlEx NTSYS PopGen MEGA AFLP SURV and TotalLab publishing and

graphic design using Adobe Photoshop Illustrator and Paint

RB Shrestha Page 10

January 26 2014

10

Publications and Presentations

1 Peer Reviewed Journal Articles

(15 totals 150 times citied) web httpscholargooglecomcitationsuser=JphkwaMAAAAJamphl=en

11 Shrestha R B and M N Parajulee 2013 Potential cotton aphid Aphis gossypii

population suppression by arthropod predators in upland cotton Journal of Insect Science

20(6)778-788

12 Parajulee M N R B Shrestha J E Slosser and D G Bordovsky 2011 Effects of

skip-row planting pattern and planting date on dryland cotton insect pest abundance and

selected plant parameters Southwestern Entomologist 36 21-39

13 Shrestha R B and M N Parajulee 2010 Effect of tillage and planting date on seasonal

abundance and diversity of predacious ground beetles in cotton Journal of Insect Science

10 (174) 1-16

14 Matis T I M N Parajulee J H Matis and R B Shrestha 2008 A mechanistic model

based analysis of cotton aphid population dynamics data Journal of Agriculture and

Forestry Ecology 10 355-362

15 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Egyptian Journal of Agricultural Research 86 57-81

16 Shrestha R B M N Parajulee O P Perera B E Scheffler and L D Densmore

2007 Characterization of microsatellite loci in the western tarnished plant bug Lygus

hesperus (Knight) (Hemiptera Miridae) Molecular Ecology Notes 7 1342-1344

17 Parajulee M N R B Shrestha and J F Leser 2006 Influence of tillage planting date

and Bt cultivar on seasonal abundance and within-plant distribution patterns of thrips and

cotton fleahoppers in cotton International Journal of Pest Management 52 249-260

18 Parajulee M N R B Shrestha and J F Leser 2006 Dispersion patterns and fixed

precision sequential sampling plans for western flower thrips (Thysanoptera Thripidae)

and cotton fleahoppers (Hemiptera Miridae) in cotton Journal of Economic Entomology

99 568-577

19 Parajulee M N R B Shrestha J F Leser D B Wester and C Blanco 2006

Evaluation of the functional response of selected cotton arthropod predators on bollworm

eggs in the laboratory and effect of temperature on their predation efficiency

Environmental Entomology 35 379-386

110 Chen F G Wu F Ge M N Parajulee and R B Shrestha 2005 Effects of elevated

CO2 and transgenic Bt cotton on plant chemistry performance and feeding of an insect

herbivore the cotton bollworm Entomologia Experimentalis et Applicata 115 341-350

RB Shrestha Page 11

January 26 2014

11

111 Dahal G R B Shrestha and R B Thapa 1997 Species composition and relative

abundance of rice green leafhopper (Nephotettix spp) in different altitude regions of

Nepal International Journal of Pest Management 43(1) 49-58

112 Dahal G A Druka T M Burns L C Villegas Z Fan R B Shrestha and R Hull

1996 Some biological and genomic properties of rice tungro bacilliform badna virus and

rice tungro spherical waika virus from Nepal Annals of Applied Biology 129 (2) 267-

287

113 Pokharel R R G Dahal and R B Shrestha 1994 Association of nematode

populations and virus-like symptoms in papaya Journal of Institute of Agriculture and

Animal Science 15 107-113

114 Dahal G R B Shrestha N K Khatri Z Fan and R Hull 1993 Incidence of virus

diseases of rice in Nepal Journal of Institute of Agriculture and Animal science 14 115-

116

115 Dahal G R R Pokharel and R B Shrestha 1992 A bibliographic database of plant

pathology in Nepal Journal of Institute of Agriculture and Animal Science 13 53-64

2 Papers Published in Refereed Proceedings (2 Total)

21 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) Proceedings World Cotton Research

Conference (CD)

22 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review pp 154 -167

Proceeding of second National Conference of Science and Technology

3 Papers in preparation for submission in Journals (5 total)

31 Shrestha R B S R K Jakka and A J Gassmann 2014 Survivorship of Western Corn

Rootworm problem field population on Bt corn hybrids revealed cross resistance between

Cry3Bb and mCry3A Journal of Economic Entomology (In preparation)

32 Shrestha R B S R K Jakka and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing eCry3A mCry3A

and eCrymCry3A stacked genes Journal of Economic Entomology (In preparation)

33 Jakka S R K R B Shrestha and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing Cry3Bb and

Cry3435Ab Journal of Economic Entomology (In preparation)

RB Shrestha Page 12

January 26 2014

12

34 Shrestha R B M N Parajulee S C Carroll J K Dever M D Arnold and J R

Hagler 2014 Characterization of the intercrop movement behavior of Lygus hesperus

between cotton and alfalfa Environmental Entomology (In preparation)

35 Shrestha R B M N Parajulee L Densmore and M San Francisco 2014

Microsatellite marker based genetic diversity of Lygus hesperus from Texas High Plains

Journal of Insect Science (In preparation)

4 Papers Published in Non-Refereed Proceedings (35 Total)

41 R B Shrestha M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

42 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

43 Parajulee M N R B Shrestha and S C Carroll 2013 Evaluation of organic pesticides

for western flower thrips management in seedling cotton Effect on plant parameters

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

44 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

45 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

46 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

47 M N Parajulee Shrestha R B W O McSpadden and SC Carroll 2011

Development of metapopulation approach for landscape-level Lygus Hesperus

management in Texas pp 220 In Proceedings of World Cotton Research Conference-5

Nov7-11 2011 Mumbai India

48 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2011 Evaluation

of varietal differences in COTMAN compensation capacity values in high-input cotton

subjected to varying levels of Lygus-induced fruit damage pp 825-829 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

RB Shrestha Page 13

January 26 2014

13

49 Parajulee M N M B Adhikari D L Kerns R B Shrestha and S C Carroll 2011

Insecticide termination rule for western tarnished plant bug in the Texas High Plains pp

1204-1208 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

410 Parajulee M N D Neupane R B Shrestha Y Yang and L T Wilson 2011 Effect

of cotton fleahopper density on pre-flower fruit loss and plant biomass in cotton pp 1095-

1099 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

411 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on Lygus hesperus abundance in adjacent cotton field A field marking study pp

853-858 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

412 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains pp 1180-88 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

413 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton pp 1155-1162 Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

414 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton pp 1163-1169 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

415 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults versus nymphs pp 849-852 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

416 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa pp 873-881

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

417 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions pp 1159-1162 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

418 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris pp 1118-1129

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

419 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip irrigated cotton pp 1251-

RB Shrestha Page 14

January 26 2014

14

1256 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

420 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus bugs from

Texas pp 1693-1699 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

421 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains pp 1700-1703

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

422 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus pp 1443-1446 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

423 Albeldantildeo W A J E Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity pp 1495-1497 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

424 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton pp 1767-1773 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

425 Parajulee M N R B Shrestha S C Carroll P L Bommireddy A M Cranmer and

K F Bronson 2005 Variable rate of irrigation water and nitrogen Potential of site-

specific management of cotton aphids pp 1735-1742 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

426 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton pp 1779-1783 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

427 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

cotton arthropod predators to bollworm eggs in the laboratory pp 1652-1659

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

428 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii p 387

Proceedings 15th

International Plant Protection Congress Foreign Language Press

Beijing China

429 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research pp 1918-1922 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

430 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Seasonal abundance patterns of thrips and fleahoppers in Texas High

RB Shrestha Page 15

January 26 2014

15

Plains cotton pp 1375-1380 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

431 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains pp 970-973 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

432 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetle in the laboratory pp 1060-1063 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

433 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in Texas High Plains pp 1370-1371 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

434 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed pp 1537-1539 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

435 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton pp 1372-1374 Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

5 Published Scientific Reports (23 Total)

51 Parajulee M N S C Carroll R B Shrestha J P Bordovsky 2012 Effect of nitrogen

fertilizer on cotton host-plant quality and its impact on arthropod activity Helms TX

2012

52 Parajulee M N R B Shrestha S Carroll and Wayne Keeling 2012 Cotton yield

response to late-season Lygus plant bug infestations as influenced by cultivar x irrigation

level treatments Lamesa TX 2012

53 Parajulee M N R B Shrestha and S C Carroll 2012 Characterization of Cotton Crop

Response to Thrips Injury for Improved Thrips Management in Texas High Plains Cotton

2012 Annual Report Cotton Incorporated Core Program Project Number 12-364

54 Parajulee M N R B Shrestha and S Carroll 2012 Development of Economic

Threshold and Management Recommendations for Lygus in Texas High Plains Cotton

2012 Annual Report (Year 1) Cotton IncorporatedTexas State Support Program Project

Number 12-112TX

55 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluating varietal

difference in COTMAN compensation capacity value in drip irrigated cotton using Lygus-

RB Shrestha Page 16

January 26 2014

16

induced fruit damage 2011 Final report Cotton Incorporated Core Program Project

Number 09-596 pp 1-16

56 Parajulee M N S C Carroll R BShrestha AM Cranmer and J P Bordovsky 2011

Influence of soil nitrogen level on seasonal activity of cotton arthropods and lint yield

under drip irrigation (field 6h) 2011 Helms Research Farm Annual Report p19

57 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluation of

westerntarnished plant bug as a late season pest of cotton 2011 Final report Cotton

IncorporatedState Support Program Project Number 08-305TX pp 1-18

58 Parajulee M N O McSpadden R B Shrestha S Carroll and W Keeling 2011

Cotton fruitingyield compensation after Lygus induced square loss as influenced by

variety x water treatments Lamesa TX 2011 Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 30-31

59 Parajulee M N B Kesey O McSpadden S Carroll R B Shrestha and W Keeling

2010 Effect of cover crop on arthropod population dynamics in subsurface drip irrigated

cotton at AG-CARES Lamesa TX 2010 Agricultural Complex for Advanced Research

and Extension Systems Annual Report pp45-46

510 Parajulee M N O McSpadden D Neupane R B Shrestha S Carroll and W

Keeling 2010 Cotton fruitingyield compensation after Lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2010 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 47-48

511 Parajulee M N R B Shrestha O McSpadden A Bastola D Neupane S Carroll and

W Keeling 2009 Cotton fruitingyield compensation after lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2009 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 46-47

512 Parajulee M N S C Carroll R BShrestha R J Kesey D M Nesmith and J P

Bordovsky 2009 Influence of soil nitrogen level on seasonal activity of cotton arthropods

and lint yield under drip irrigation 2009 Helms Research Farm Annual Report p18

513 Parajulee M N A Bastola R B Shrestha and W Keeling 2009 Evaluation of cotton

boll susceptibility to Lygus bugs as influenced by cultivar x irrigation interaction

AGCARES Lamesa TX 2009Agricultural Complex for Advanced Research and

Extension Systems Annual Report pp 48-49

514 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2004 Influence of soil nitrogen level on seasonal activity of cotton arthropods in drip

irrigation system Helms Research Farm Annual Report p 25

515 Parajulee M N J F Leser R B Shrestha A K Barman and R K Boman 2004

Evaluating the merits of sub-threshold effects of the Bollgard technology on the

economics of bollworm management Helms Research Farm Annual Report p 14

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 2: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 2

January 26 2014

2

May 2002-Dec 2009 Graduate Research Assistant Cotton Entomology Program Texas AampM

AgriLife Research Lubbock TX

Jul 1994-May 2002 Plant Protection Officer Division of Agriculture Ministry of Agriculture

Kathmandu Nepal

Jan 1992-May 1994 Research Assistant Department of Plant Pathology Institute of Agriculture

and Animal Sciences Rampur Nepal

Immigration Status

Permanent Resident (authorized to work in the United States)

Summary of Scientific Publications (authoredco-authored see pp 16-26 for detailed)

Publication Category Number Published

Peer-reviewed journal articles 15

Refereed proceeding papers 2

Non-refereed proceeding papers 35

Project reports 24

Scientific abstracts 23

Papers in preparationinternal review 5

Career total 99 (published only)

Note My 15 peer reviewed journal papers has been cited 148 times from scientist

all over the world (from 17 different countries)

Summary of Scientific Presentations (see pp 26-35 for detailed list)

Presentation Category Number Presented

International invited oral 9

National invited oral 13

National submitted oral 33

International poster 8

National poster 36

Career total 99

RB Shrestha Page 3

January 26 2014

3

Teaching Student Advisory and Supervision at Texas Tech University

Teaching Involved in syllabus development lecturing in class and posting lecture slides and

other teaching material online in WebCT and Blackboard online server grading exams

and evaluating student performance

2011 ldquoIntroductory Entomologyrdquo undergraduate level online course fall semester

2011 ldquoAdvanced Insect Ecologyrdquo graduate level spring semester

2010 Invited guest lecturer for Integrated Pest management graduate level course spring

2009 ldquoAdvanced Insect Ecologyrdquo graduate level spring semester

Graduate Advisory Served in academic advisory committee of a graduate student (Angela

Fernando MS in Crop Protection Department of Plant and Soil Science Texas Tech

University) Thesis title ldquoOverwintering Biology of Lygus hesperus in the Texas High

Plainsrdquo

Student Supervised Provided technical guidance to MS level students in the program Trained

6 junior MS students in our laboratory Helped in experimental design data

collection and analysis in their MS thesis research projects Supervised 2

undergraduate research fellows and many summer student assistants

Informal Teaching gt400 in field one day-long growersrsquo training and demonstration sessions on

various aspects of crop protection in Nepal

Grants Honors Awards Fellowships and Scholarships (total $185624)

2013 Honor for outstanding leadershipndashplaque presented by Society of Overseas Nepalese

Entomologist (SONE) in annual business meeting of SONE November 11 2013

Austin Texas

2013 Honor in recognition of dedicated service- plaque presented by Cotton Entomology

Program Texas AampM AgriLife Research Center Lubbock TX in May 24 2013

2013 CO-PI Cotton Incorporated - Texas State Support Committee competitive grant

(Characterization of Cotton Crop Response to Thrips Injury for Improved Thrips

Management in Texas High Plains Cotton) $27000 per year (2012-2014 funded

annually)

2013 CO-PI Cotton Incorporated - Texas State Support Committee competitive grant

(Development of Economic Threshold and Management Recommendations for Lygus

in Texas High Plains Cotton) $30000 per year (2012-2014 funded annually)

2012 New career professional development (MONSANTO sponsored) travel assistantship

award by ESA ($424)

RB Shrestha Page 4

January 26 2014

4

2012 CO-PI Cotton Incorporated - Texas State Support Committee competitive grant

(Characterization of Cotton Crop Response to Thrips Injury for Improved Thrips

Management in Texas High Plains Cotton) $27000 per year (2012-2014 funded

annually)

2012 CO-PI Cotton Incorporated - Texas State Support Committee competitive grant

(Development of Economic Threshold and Management Recommendations for Lygus

in Texas High Plains Cotton) $30000 per year (2012-2014 funded annually)

2010 CO-PI International Cotton Research Center competitive grant (Lygus Bugs

Investigation of Predisposition Factors Causing Outbreaks in Order to Strengthen

Cotton Pest Management Strategies) $65000 (2010-2011)

2010 Travel grant from Biological Science Department and Graduate School TTU for

participation at 58th

annual meeting of ESA San Diego CA (Dec 12-15) ($800)

2009 Travel grant from Biological Science Department and Graduate School TTU for

participation and presentation at 57th

annual meeting of ESA Indianapolis IN (Dec

13-16) ($1000)

2009 Third place oral presentation award for student competition at Entomological Society

of America Southwestern Branch Meeting Stillwater OK (Feb 23-26)

2008 Travel grant from Biological Science Department and Graduate School for

participation at 56th

annual meeting of ESA Reno NV (Nov 16-19) ($1000)

2007 Travel grant from Biological Science Department and Graduate School TTU for

participation at 55th

annual meeting of ESA San Diego CA (Dec 9-12) ($700)

2006 Travel grant from Biological Science Department and Graduate School TTU for

participation at 54th

annual meeting of ESA Indianapolis IN (Dec 10-13) ($700)

2003 West Texas Agricultural Chemicals Institute ($1000 scholarship award)

2003 Leland Chandler Endowed Scholarship from Graduate School TTU ($500)

2003 Harold and Mary Dregne Graduate Scholarship from Graduate School TTU ($500)

2001 Netherlands Fellowship Program International Training Course on IPM in IAC

Wageningen the Netherlands (March 18-June 30)

2000 UNDP-IPM Training Fellowship International Training Course on crop protection in

CICAT Guangzhou China (May 5 to July 4)

1998 FAO IPM project in rice Trainers Training Fellowship Trainers Training on IPM in

rice in Dhanusa Nepal (April 6 to July 10)

1993 USAIDNepal Diploma in Biotechnology Training Fellowship Laboratory Training

on Plant Biotechnology in RLABB Kathmandu Nepal (Feb 13-May 13)

RB Shrestha Page 5

January 26 2014

5

Peer Review Service for Entomology Journals

I have reviewed many scientific papers for following entomological journals

Journal of Insect Science

Journal of Economic Entomology

Environmental Entomology

Neotropical Entomology

Entomologia Experimentalis et Applicata

Extension Experience in the USA

During my graduate programs (Master and PhD) from 2002 to 2011 I worked as graduate

research assistant in Texas AampM AgriLife Research and Extension Center Lubbock During this

period I got opportunity to interact closely with many extension specialists and research scientists

IPM and county agriculture extension agents growers crop consultants industry representatives

university professors and USDA scientists I have participated in many agricultural extension

activities Dr Jim Leser (Professor Extension Entomology in Texas AampM) was my masters

graduate advisory committee member I have closely worked with Dr David Kerns (Associate

Professor Extension Entomology in Texas AampM) in various collaborative extension projects

Below are the lists of some of my extension experience gained in Lubbock Texas

Participated in a Corn Rootworm Field day in Boon Iowa August 8 2013

Participated and given invited presentation in 3 crop consultant meetings in Texas

Participated and given invited talks in 4 plains cotton growers and cotton incorporated

annual meetings

Participated and given invited talk in 1 county extension agents refresher training

conferences

Participated in 5 field days and turn row meetings

Participated in 4 industries annual technology update meetings

Organized 4 insect expo insect zoo in science spectrum museum

Conducted many product evaluation field trials in growers field

Attended many commodity group (plains cotton growers advisory group) meetings

Given gt80 presentations for various audience in many meeting conferences

symposium workshops

Guided 3 group of international delegates and scientist visit or tours

Given 1 invited talk in research-extension interaction science conference

RB Shrestha Page 6

January 26 2014

6

Conducted many season-long insect pest sampling or scouting in various experimental

fields for many extension faculties collaborators

Conducted product evaluation field research in collaboration of various industries

Given 1 invited guest lectures for graduate and undergraduate classes in Texas Tech

University

Collaborated with many Extension specialists (Extension entomologists Irrigation

engineers Weed scientistAgronomist Pathologists Economists Plant breeders etc) on

various extension program and field research

Collaboration with many IPM agents county extension agents crop consultants USDA

scientists University professors and many graduate undergraduate students

Collaborated with many entomology faculties from various universities

Organized and moderated symposium in national and regional levels entomology

society meetings

Taught 3 graduate and undergraduate level entomology courses in university

Delivered entomology science and career demonstrations tour and presentations to

many groups of middle school students undergraduate students and upper bound

programrsquos students

Extension Experience in Nepal

I was a Crop Protection Extension Officer in the Department of Agriculture Nepal for 8 years

(1994-2002) I was the team leader of crop protection Extension programs in the district The goal

of my crop protection program was to disseminate modern crop protection technologies to

growers As a district IPM coordinator and crop protection officers I had managed district level

IPM program with the help of 15 junior field technicians and technical associates Examples of

extension activities conducted are as follows

Training and talks gt400 in-field training sessions at grower meetings

Field product evaluation and result demonstrations gt400 crop protection

demonstrations

Mass communications Conducted a radio talk program on rice blast disease and two

TV talk programs on pest management in mushroom cultivation in Nepal

Field days Organized four farmersrsquo field day

Farmersrsquo field schools Conducted three IPM farmersrsquo field schools in rice and cole

crops

Crop disease and insect pest diagnostic service

Pesticide regulation implementation Activity included pesticide registration resellers

training and pesticide inspection

Crop protection program planning monitoring and evaluation (regional and national)

RB Shrestha Page 7

January 26 2014

7

Postharvest loss management program such as grain storage improvement zero-energy

potato storage and cellar fruit storage

Training of junior agriculture technicians and progressive lead farmers

Distribution of posters leaflets booklets pamphlets and calendars

Crop protection training kits and subsidies distribution

Collaboration with research scientists in National Agriculture Research Council for

technology update and feedback on new crop protection technologies

Annual progress report publication (1995-2001)

Growers competition and award program

Collaborative Team Work Experiences

I have worked in close collaboration with several scientists and people from other national and

international laboratories and have published scientific papers coauthored with more than 30

people from different institutions

Leadership and Service to Professional Societies

Contributions to Professional Societies

2013 ESA Annual Meeting Member Symposium organizer Austin TX (November 10-13)

2013 ESA Annual Meeting Graduate Student Ten-Minute Paper competition session

moderator Austin TX (November 10-13) P-IE Section

Monday November 11 2013 800 AM-1200 PM

2012-2013 President Society of Overseas Nepalese Entomologists (SONE)

2012 ESA Annual Meeting Member Symposium organizer Knoxville TN (Nov 11-14)

2012 ESA Annual Meeting Symposium moderator Knoxville TN (November 11-14) P-IE

Section Environmental Entomology Symposium Tuesday November 13 2012 800

AM-1215 PM

2011-2012 Vice president Society of Overseas Nepalese Entomologists (SONE)

2011 ESA Annual Meeting Student Oral Competition Judge Reno NV (November 13-16)

2010 ESA Annual Meeting Symposium Organizer and Moderator San Diego CA

(December 12-16)

2010 ESA Annual Meeting Student Volunteer San Diego CA (December 12-16)

2009 ESA-SWB Annual Meeting Student Volunteer Stillwater OK (February 23-26)

2009 ESA-SWB Student Affairs Committee Member

2009 ESA Annual Meeting Student Volunteer Indianapolis IN (December 12-16)

RB Shrestha Page 8

January 26 2014

8

2009 ESA Annual Meeting Student Poster Competition Judge Indianapolis IN (December

12-16)

2008 ESA Annual Meeting Student Volunteer Reno NV (December 12-16)

2006 ESA-SWB Annual Meeting Student Volunteer Austin TX (February 28-March 2)

2006 ESA-SWB Annual Meeting Student Poster Competition Judge Austin TX (February

28-March 2)

2004 ESA-SWB Annual Meeting Student Volunteer Lubbock TX (February 23-26)

2004 ESA-SWB Annual Meeting Student Affairs Committee Member Lubbock TX

(February 23-26)

Conference Symposium Management

2014 Shrestha R B S R K Jakka and A J Gassmann 2014 co-organizing a symposium

(proposal accepted) in ESA NCB Annual Meeting Proposed title Advancements and

Challenges in Western Corn Rootworm Management Des Moines IA (March 9-10

2014)

2013 Shrestha R B B K Gautam J Rijal and S Gautam 2013 Jointly organized and

moderated Member symposium on ldquoPromoting Entomological Collaboration through

Inter-societal Network and Information Sharingrdquo for the 61th

annual meeting of ESA

Austin TX (November 10-13) Tuesday November 12 2013 600 PM-1000 PM

2012 Shrestha R B and R R Pandey 2012 Jointly organized and moderated Member

symposium on ldquoGlobal Collaboration for the Development of Next Generation IPM

Programrdquo for the 60th

annual meeting of ESA Knoxville TN (November 11-14)

Wednesday November 14 2012 800 AM-1200 PM

2010 Shrestha R B and M N Parajulee 2010 Jointly organized and moderated Member

symposium on ldquoMolecular and Other Novel Tools in Ecological Researchrdquo for the 58th

annual meeting of ESA San Diego CA (December 12-15) Wednesday December 15

2010 115 PM-330 PM

Active Memberships in Professional and Honor Societies

Member of Entomological Society of America

Member of Ecological Society of America

Member of Society of Southwestern Entomologists

Member of High Plains Association of Crop Consultants

President of Society of Overseas Nepalese Entomologists

Member of the National Scholars Honor Society

Member of Golden Key International Honor Society TTU Chapter

Member of Honor Society of Agriculture Gamma Sigma Delta TTU Chapter

RB Shrestha Page 9

January 26 2014

9

Member of American Association for Advancement in Science

Founder Member of Nepalese Student Association Texas Tech University

Professional Development Trainings and Workshops

In addition to my formal academic courses I have learned various important skills through

thirteen professional development trainings and workshops The training includes grant

writing training computer programing (R and Python) Integrated Pest Management (IPM)

training Training of Trainers (TOT) and plant biotechnology training Immuno-marking and

Enzyme Linked Immuno Sorbent (ELISA) training microsatellite DNA marker discovery

training teaching enhancement (Blackboard and WebCT) training laboratory safety (handling

radioactive materials pesticide application and shipment of hazardous materials) Compliance

training for conducting regulated Monsanto cotton field trial and training on cotton growth and

development monitoring using COTMAN software

I have also participated in short training courses in professional development such as time

management supervision project management problem solving conflict management scientific

methods information security awareness work place ethics creating discrimination free work

spaces and reporting fraud waste and abuse

Computational or Quantitative Skills and Experience

Through formal course work short training work experience and self-learning exercises I have

acquired computer skills on the application of 29 different computer software and programs

and used them in various research projects My computational skills include statistical data

analysis using SAS SPSS MATLAB SigmaPlot and ArcGIS online course preparation and

teaching using Blackboard and WebCT integrated reference management using EndNote

Medley and Reference Manager documentation database management and technical

communication using Microsoft Office Word Excel Access PowerPoint and Outlook

population genetics and phylogenetic data analysis using PAUP PHYLIP ARLEQUIN

STRUCTURE GenAlEx NTSYS PopGen MEGA AFLP SURV and TotalLab publishing and

graphic design using Adobe Photoshop Illustrator and Paint

RB Shrestha Page 10

January 26 2014

10

Publications and Presentations

1 Peer Reviewed Journal Articles

(15 totals 150 times citied) web httpscholargooglecomcitationsuser=JphkwaMAAAAJamphl=en

11 Shrestha R B and M N Parajulee 2013 Potential cotton aphid Aphis gossypii

population suppression by arthropod predators in upland cotton Journal of Insect Science

20(6)778-788

12 Parajulee M N R B Shrestha J E Slosser and D G Bordovsky 2011 Effects of

skip-row planting pattern and planting date on dryland cotton insect pest abundance and

selected plant parameters Southwestern Entomologist 36 21-39

13 Shrestha R B and M N Parajulee 2010 Effect of tillage and planting date on seasonal

abundance and diversity of predacious ground beetles in cotton Journal of Insect Science

10 (174) 1-16

14 Matis T I M N Parajulee J H Matis and R B Shrestha 2008 A mechanistic model

based analysis of cotton aphid population dynamics data Journal of Agriculture and

Forestry Ecology 10 355-362

15 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Egyptian Journal of Agricultural Research 86 57-81

16 Shrestha R B M N Parajulee O P Perera B E Scheffler and L D Densmore

2007 Characterization of microsatellite loci in the western tarnished plant bug Lygus

hesperus (Knight) (Hemiptera Miridae) Molecular Ecology Notes 7 1342-1344

17 Parajulee M N R B Shrestha and J F Leser 2006 Influence of tillage planting date

and Bt cultivar on seasonal abundance and within-plant distribution patterns of thrips and

cotton fleahoppers in cotton International Journal of Pest Management 52 249-260

18 Parajulee M N R B Shrestha and J F Leser 2006 Dispersion patterns and fixed

precision sequential sampling plans for western flower thrips (Thysanoptera Thripidae)

and cotton fleahoppers (Hemiptera Miridae) in cotton Journal of Economic Entomology

99 568-577

19 Parajulee M N R B Shrestha J F Leser D B Wester and C Blanco 2006

Evaluation of the functional response of selected cotton arthropod predators on bollworm

eggs in the laboratory and effect of temperature on their predation efficiency

Environmental Entomology 35 379-386

110 Chen F G Wu F Ge M N Parajulee and R B Shrestha 2005 Effects of elevated

CO2 and transgenic Bt cotton on plant chemistry performance and feeding of an insect

herbivore the cotton bollworm Entomologia Experimentalis et Applicata 115 341-350

RB Shrestha Page 11

January 26 2014

11

111 Dahal G R B Shrestha and R B Thapa 1997 Species composition and relative

abundance of rice green leafhopper (Nephotettix spp) in different altitude regions of

Nepal International Journal of Pest Management 43(1) 49-58

112 Dahal G A Druka T M Burns L C Villegas Z Fan R B Shrestha and R Hull

1996 Some biological and genomic properties of rice tungro bacilliform badna virus and

rice tungro spherical waika virus from Nepal Annals of Applied Biology 129 (2) 267-

287

113 Pokharel R R G Dahal and R B Shrestha 1994 Association of nematode

populations and virus-like symptoms in papaya Journal of Institute of Agriculture and

Animal Science 15 107-113

114 Dahal G R B Shrestha N K Khatri Z Fan and R Hull 1993 Incidence of virus

diseases of rice in Nepal Journal of Institute of Agriculture and Animal science 14 115-

116

115 Dahal G R R Pokharel and R B Shrestha 1992 A bibliographic database of plant

pathology in Nepal Journal of Institute of Agriculture and Animal Science 13 53-64

2 Papers Published in Refereed Proceedings (2 Total)

21 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) Proceedings World Cotton Research

Conference (CD)

22 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review pp 154 -167

Proceeding of second National Conference of Science and Technology

3 Papers in preparation for submission in Journals (5 total)

31 Shrestha R B S R K Jakka and A J Gassmann 2014 Survivorship of Western Corn

Rootworm problem field population on Bt corn hybrids revealed cross resistance between

Cry3Bb and mCry3A Journal of Economic Entomology (In preparation)

32 Shrestha R B S R K Jakka and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing eCry3A mCry3A

and eCrymCry3A stacked genes Journal of Economic Entomology (In preparation)

33 Jakka S R K R B Shrestha and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing Cry3Bb and

Cry3435Ab Journal of Economic Entomology (In preparation)

RB Shrestha Page 12

January 26 2014

12

34 Shrestha R B M N Parajulee S C Carroll J K Dever M D Arnold and J R

Hagler 2014 Characterization of the intercrop movement behavior of Lygus hesperus

between cotton and alfalfa Environmental Entomology (In preparation)

35 Shrestha R B M N Parajulee L Densmore and M San Francisco 2014

Microsatellite marker based genetic diversity of Lygus hesperus from Texas High Plains

Journal of Insect Science (In preparation)

4 Papers Published in Non-Refereed Proceedings (35 Total)

41 R B Shrestha M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

42 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

43 Parajulee M N R B Shrestha and S C Carroll 2013 Evaluation of organic pesticides

for western flower thrips management in seedling cotton Effect on plant parameters

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

44 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

45 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

46 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

47 M N Parajulee Shrestha R B W O McSpadden and SC Carroll 2011

Development of metapopulation approach for landscape-level Lygus Hesperus

management in Texas pp 220 In Proceedings of World Cotton Research Conference-5

Nov7-11 2011 Mumbai India

48 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2011 Evaluation

of varietal differences in COTMAN compensation capacity values in high-input cotton

subjected to varying levels of Lygus-induced fruit damage pp 825-829 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

RB Shrestha Page 13

January 26 2014

13

49 Parajulee M N M B Adhikari D L Kerns R B Shrestha and S C Carroll 2011

Insecticide termination rule for western tarnished plant bug in the Texas High Plains pp

1204-1208 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

410 Parajulee M N D Neupane R B Shrestha Y Yang and L T Wilson 2011 Effect

of cotton fleahopper density on pre-flower fruit loss and plant biomass in cotton pp 1095-

1099 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

411 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on Lygus hesperus abundance in adjacent cotton field A field marking study pp

853-858 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

412 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains pp 1180-88 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

413 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton pp 1155-1162 Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

414 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton pp 1163-1169 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

415 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults versus nymphs pp 849-852 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

416 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa pp 873-881

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

417 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions pp 1159-1162 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

418 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris pp 1118-1129

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

419 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip irrigated cotton pp 1251-

RB Shrestha Page 14

January 26 2014

14

1256 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

420 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus bugs from

Texas pp 1693-1699 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

421 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains pp 1700-1703

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

422 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus pp 1443-1446 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

423 Albeldantildeo W A J E Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity pp 1495-1497 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

424 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton pp 1767-1773 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

425 Parajulee M N R B Shrestha S C Carroll P L Bommireddy A M Cranmer and

K F Bronson 2005 Variable rate of irrigation water and nitrogen Potential of site-

specific management of cotton aphids pp 1735-1742 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

426 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton pp 1779-1783 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

427 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

cotton arthropod predators to bollworm eggs in the laboratory pp 1652-1659

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

428 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii p 387

Proceedings 15th

International Plant Protection Congress Foreign Language Press

Beijing China

429 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research pp 1918-1922 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

430 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Seasonal abundance patterns of thrips and fleahoppers in Texas High

RB Shrestha Page 15

January 26 2014

15

Plains cotton pp 1375-1380 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

431 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains pp 970-973 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

432 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetle in the laboratory pp 1060-1063 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

433 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in Texas High Plains pp 1370-1371 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

434 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed pp 1537-1539 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

435 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton pp 1372-1374 Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

5 Published Scientific Reports (23 Total)

51 Parajulee M N S C Carroll R B Shrestha J P Bordovsky 2012 Effect of nitrogen

fertilizer on cotton host-plant quality and its impact on arthropod activity Helms TX

2012

52 Parajulee M N R B Shrestha S Carroll and Wayne Keeling 2012 Cotton yield

response to late-season Lygus plant bug infestations as influenced by cultivar x irrigation

level treatments Lamesa TX 2012

53 Parajulee M N R B Shrestha and S C Carroll 2012 Characterization of Cotton Crop

Response to Thrips Injury for Improved Thrips Management in Texas High Plains Cotton

2012 Annual Report Cotton Incorporated Core Program Project Number 12-364

54 Parajulee M N R B Shrestha and S Carroll 2012 Development of Economic

Threshold and Management Recommendations for Lygus in Texas High Plains Cotton

2012 Annual Report (Year 1) Cotton IncorporatedTexas State Support Program Project

Number 12-112TX

55 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluating varietal

difference in COTMAN compensation capacity value in drip irrigated cotton using Lygus-

RB Shrestha Page 16

January 26 2014

16

induced fruit damage 2011 Final report Cotton Incorporated Core Program Project

Number 09-596 pp 1-16

56 Parajulee M N S C Carroll R BShrestha AM Cranmer and J P Bordovsky 2011

Influence of soil nitrogen level on seasonal activity of cotton arthropods and lint yield

under drip irrigation (field 6h) 2011 Helms Research Farm Annual Report p19

57 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluation of

westerntarnished plant bug as a late season pest of cotton 2011 Final report Cotton

IncorporatedState Support Program Project Number 08-305TX pp 1-18

58 Parajulee M N O McSpadden R B Shrestha S Carroll and W Keeling 2011

Cotton fruitingyield compensation after Lygus induced square loss as influenced by

variety x water treatments Lamesa TX 2011 Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 30-31

59 Parajulee M N B Kesey O McSpadden S Carroll R B Shrestha and W Keeling

2010 Effect of cover crop on arthropod population dynamics in subsurface drip irrigated

cotton at AG-CARES Lamesa TX 2010 Agricultural Complex for Advanced Research

and Extension Systems Annual Report pp45-46

510 Parajulee M N O McSpadden D Neupane R B Shrestha S Carroll and W

Keeling 2010 Cotton fruitingyield compensation after Lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2010 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 47-48

511 Parajulee M N R B Shrestha O McSpadden A Bastola D Neupane S Carroll and

W Keeling 2009 Cotton fruitingyield compensation after lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2009 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 46-47

512 Parajulee M N S C Carroll R BShrestha R J Kesey D M Nesmith and J P

Bordovsky 2009 Influence of soil nitrogen level on seasonal activity of cotton arthropods

and lint yield under drip irrigation 2009 Helms Research Farm Annual Report p18

513 Parajulee M N A Bastola R B Shrestha and W Keeling 2009 Evaluation of cotton

boll susceptibility to Lygus bugs as influenced by cultivar x irrigation interaction

AGCARES Lamesa TX 2009Agricultural Complex for Advanced Research and

Extension Systems Annual Report pp 48-49

514 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2004 Influence of soil nitrogen level on seasonal activity of cotton arthropods in drip

irrigation system Helms Research Farm Annual Report p 25

515 Parajulee M N J F Leser R B Shrestha A K Barman and R K Boman 2004

Evaluating the merits of sub-threshold effects of the Bollgard technology on the

economics of bollworm management Helms Research Farm Annual Report p 14

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 3: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 3

January 26 2014

3

Teaching Student Advisory and Supervision at Texas Tech University

Teaching Involved in syllabus development lecturing in class and posting lecture slides and

other teaching material online in WebCT and Blackboard online server grading exams

and evaluating student performance

2011 ldquoIntroductory Entomologyrdquo undergraduate level online course fall semester

2011 ldquoAdvanced Insect Ecologyrdquo graduate level spring semester

2010 Invited guest lecturer for Integrated Pest management graduate level course spring

2009 ldquoAdvanced Insect Ecologyrdquo graduate level spring semester

Graduate Advisory Served in academic advisory committee of a graduate student (Angela

Fernando MS in Crop Protection Department of Plant and Soil Science Texas Tech

University) Thesis title ldquoOverwintering Biology of Lygus hesperus in the Texas High

Plainsrdquo

Student Supervised Provided technical guidance to MS level students in the program Trained

6 junior MS students in our laboratory Helped in experimental design data

collection and analysis in their MS thesis research projects Supervised 2

undergraduate research fellows and many summer student assistants

Informal Teaching gt400 in field one day-long growersrsquo training and demonstration sessions on

various aspects of crop protection in Nepal

Grants Honors Awards Fellowships and Scholarships (total $185624)

2013 Honor for outstanding leadershipndashplaque presented by Society of Overseas Nepalese

Entomologist (SONE) in annual business meeting of SONE November 11 2013

Austin Texas

2013 Honor in recognition of dedicated service- plaque presented by Cotton Entomology

Program Texas AampM AgriLife Research Center Lubbock TX in May 24 2013

2013 CO-PI Cotton Incorporated - Texas State Support Committee competitive grant

(Characterization of Cotton Crop Response to Thrips Injury for Improved Thrips

Management in Texas High Plains Cotton) $27000 per year (2012-2014 funded

annually)

2013 CO-PI Cotton Incorporated - Texas State Support Committee competitive grant

(Development of Economic Threshold and Management Recommendations for Lygus

in Texas High Plains Cotton) $30000 per year (2012-2014 funded annually)

2012 New career professional development (MONSANTO sponsored) travel assistantship

award by ESA ($424)

RB Shrestha Page 4

January 26 2014

4

2012 CO-PI Cotton Incorporated - Texas State Support Committee competitive grant

(Characterization of Cotton Crop Response to Thrips Injury for Improved Thrips

Management in Texas High Plains Cotton) $27000 per year (2012-2014 funded

annually)

2012 CO-PI Cotton Incorporated - Texas State Support Committee competitive grant

(Development of Economic Threshold and Management Recommendations for Lygus

in Texas High Plains Cotton) $30000 per year (2012-2014 funded annually)

2010 CO-PI International Cotton Research Center competitive grant (Lygus Bugs

Investigation of Predisposition Factors Causing Outbreaks in Order to Strengthen

Cotton Pest Management Strategies) $65000 (2010-2011)

2010 Travel grant from Biological Science Department and Graduate School TTU for

participation at 58th

annual meeting of ESA San Diego CA (Dec 12-15) ($800)

2009 Travel grant from Biological Science Department and Graduate School TTU for

participation and presentation at 57th

annual meeting of ESA Indianapolis IN (Dec

13-16) ($1000)

2009 Third place oral presentation award for student competition at Entomological Society

of America Southwestern Branch Meeting Stillwater OK (Feb 23-26)

2008 Travel grant from Biological Science Department and Graduate School for

participation at 56th

annual meeting of ESA Reno NV (Nov 16-19) ($1000)

2007 Travel grant from Biological Science Department and Graduate School TTU for

participation at 55th

annual meeting of ESA San Diego CA (Dec 9-12) ($700)

2006 Travel grant from Biological Science Department and Graduate School TTU for

participation at 54th

annual meeting of ESA Indianapolis IN (Dec 10-13) ($700)

2003 West Texas Agricultural Chemicals Institute ($1000 scholarship award)

2003 Leland Chandler Endowed Scholarship from Graduate School TTU ($500)

2003 Harold and Mary Dregne Graduate Scholarship from Graduate School TTU ($500)

2001 Netherlands Fellowship Program International Training Course on IPM in IAC

Wageningen the Netherlands (March 18-June 30)

2000 UNDP-IPM Training Fellowship International Training Course on crop protection in

CICAT Guangzhou China (May 5 to July 4)

1998 FAO IPM project in rice Trainers Training Fellowship Trainers Training on IPM in

rice in Dhanusa Nepal (April 6 to July 10)

1993 USAIDNepal Diploma in Biotechnology Training Fellowship Laboratory Training

on Plant Biotechnology in RLABB Kathmandu Nepal (Feb 13-May 13)

RB Shrestha Page 5

January 26 2014

5

Peer Review Service for Entomology Journals

I have reviewed many scientific papers for following entomological journals

Journal of Insect Science

Journal of Economic Entomology

Environmental Entomology

Neotropical Entomology

Entomologia Experimentalis et Applicata

Extension Experience in the USA

During my graduate programs (Master and PhD) from 2002 to 2011 I worked as graduate

research assistant in Texas AampM AgriLife Research and Extension Center Lubbock During this

period I got opportunity to interact closely with many extension specialists and research scientists

IPM and county agriculture extension agents growers crop consultants industry representatives

university professors and USDA scientists I have participated in many agricultural extension

activities Dr Jim Leser (Professor Extension Entomology in Texas AampM) was my masters

graduate advisory committee member I have closely worked with Dr David Kerns (Associate

Professor Extension Entomology in Texas AampM) in various collaborative extension projects

Below are the lists of some of my extension experience gained in Lubbock Texas

Participated in a Corn Rootworm Field day in Boon Iowa August 8 2013

Participated and given invited presentation in 3 crop consultant meetings in Texas

Participated and given invited talks in 4 plains cotton growers and cotton incorporated

annual meetings

Participated and given invited talk in 1 county extension agents refresher training

conferences

Participated in 5 field days and turn row meetings

Participated in 4 industries annual technology update meetings

Organized 4 insect expo insect zoo in science spectrum museum

Conducted many product evaluation field trials in growers field

Attended many commodity group (plains cotton growers advisory group) meetings

Given gt80 presentations for various audience in many meeting conferences

symposium workshops

Guided 3 group of international delegates and scientist visit or tours

Given 1 invited talk in research-extension interaction science conference

RB Shrestha Page 6

January 26 2014

6

Conducted many season-long insect pest sampling or scouting in various experimental

fields for many extension faculties collaborators

Conducted product evaluation field research in collaboration of various industries

Given 1 invited guest lectures for graduate and undergraduate classes in Texas Tech

University

Collaborated with many Extension specialists (Extension entomologists Irrigation

engineers Weed scientistAgronomist Pathologists Economists Plant breeders etc) on

various extension program and field research

Collaboration with many IPM agents county extension agents crop consultants USDA

scientists University professors and many graduate undergraduate students

Collaborated with many entomology faculties from various universities

Organized and moderated symposium in national and regional levels entomology

society meetings

Taught 3 graduate and undergraduate level entomology courses in university

Delivered entomology science and career demonstrations tour and presentations to

many groups of middle school students undergraduate students and upper bound

programrsquos students

Extension Experience in Nepal

I was a Crop Protection Extension Officer in the Department of Agriculture Nepal for 8 years

(1994-2002) I was the team leader of crop protection Extension programs in the district The goal

of my crop protection program was to disseminate modern crop protection technologies to

growers As a district IPM coordinator and crop protection officers I had managed district level

IPM program with the help of 15 junior field technicians and technical associates Examples of

extension activities conducted are as follows

Training and talks gt400 in-field training sessions at grower meetings

Field product evaluation and result demonstrations gt400 crop protection

demonstrations

Mass communications Conducted a radio talk program on rice blast disease and two

TV talk programs on pest management in mushroom cultivation in Nepal

Field days Organized four farmersrsquo field day

Farmersrsquo field schools Conducted three IPM farmersrsquo field schools in rice and cole

crops

Crop disease and insect pest diagnostic service

Pesticide regulation implementation Activity included pesticide registration resellers

training and pesticide inspection

Crop protection program planning monitoring and evaluation (regional and national)

RB Shrestha Page 7

January 26 2014

7

Postharvest loss management program such as grain storage improvement zero-energy

potato storage and cellar fruit storage

Training of junior agriculture technicians and progressive lead farmers

Distribution of posters leaflets booklets pamphlets and calendars

Crop protection training kits and subsidies distribution

Collaboration with research scientists in National Agriculture Research Council for

technology update and feedback on new crop protection technologies

Annual progress report publication (1995-2001)

Growers competition and award program

Collaborative Team Work Experiences

I have worked in close collaboration with several scientists and people from other national and

international laboratories and have published scientific papers coauthored with more than 30

people from different institutions

Leadership and Service to Professional Societies

Contributions to Professional Societies

2013 ESA Annual Meeting Member Symposium organizer Austin TX (November 10-13)

2013 ESA Annual Meeting Graduate Student Ten-Minute Paper competition session

moderator Austin TX (November 10-13) P-IE Section

Monday November 11 2013 800 AM-1200 PM

2012-2013 President Society of Overseas Nepalese Entomologists (SONE)

2012 ESA Annual Meeting Member Symposium organizer Knoxville TN (Nov 11-14)

2012 ESA Annual Meeting Symposium moderator Knoxville TN (November 11-14) P-IE

Section Environmental Entomology Symposium Tuesday November 13 2012 800

AM-1215 PM

2011-2012 Vice president Society of Overseas Nepalese Entomologists (SONE)

2011 ESA Annual Meeting Student Oral Competition Judge Reno NV (November 13-16)

2010 ESA Annual Meeting Symposium Organizer and Moderator San Diego CA

(December 12-16)

2010 ESA Annual Meeting Student Volunteer San Diego CA (December 12-16)

2009 ESA-SWB Annual Meeting Student Volunteer Stillwater OK (February 23-26)

2009 ESA-SWB Student Affairs Committee Member

2009 ESA Annual Meeting Student Volunteer Indianapolis IN (December 12-16)

RB Shrestha Page 8

January 26 2014

8

2009 ESA Annual Meeting Student Poster Competition Judge Indianapolis IN (December

12-16)

2008 ESA Annual Meeting Student Volunteer Reno NV (December 12-16)

2006 ESA-SWB Annual Meeting Student Volunteer Austin TX (February 28-March 2)

2006 ESA-SWB Annual Meeting Student Poster Competition Judge Austin TX (February

28-March 2)

2004 ESA-SWB Annual Meeting Student Volunteer Lubbock TX (February 23-26)

2004 ESA-SWB Annual Meeting Student Affairs Committee Member Lubbock TX

(February 23-26)

Conference Symposium Management

2014 Shrestha R B S R K Jakka and A J Gassmann 2014 co-organizing a symposium

(proposal accepted) in ESA NCB Annual Meeting Proposed title Advancements and

Challenges in Western Corn Rootworm Management Des Moines IA (March 9-10

2014)

2013 Shrestha R B B K Gautam J Rijal and S Gautam 2013 Jointly organized and

moderated Member symposium on ldquoPromoting Entomological Collaboration through

Inter-societal Network and Information Sharingrdquo for the 61th

annual meeting of ESA

Austin TX (November 10-13) Tuesday November 12 2013 600 PM-1000 PM

2012 Shrestha R B and R R Pandey 2012 Jointly organized and moderated Member

symposium on ldquoGlobal Collaboration for the Development of Next Generation IPM

Programrdquo for the 60th

annual meeting of ESA Knoxville TN (November 11-14)

Wednesday November 14 2012 800 AM-1200 PM

2010 Shrestha R B and M N Parajulee 2010 Jointly organized and moderated Member

symposium on ldquoMolecular and Other Novel Tools in Ecological Researchrdquo for the 58th

annual meeting of ESA San Diego CA (December 12-15) Wednesday December 15

2010 115 PM-330 PM

Active Memberships in Professional and Honor Societies

Member of Entomological Society of America

Member of Ecological Society of America

Member of Society of Southwestern Entomologists

Member of High Plains Association of Crop Consultants

President of Society of Overseas Nepalese Entomologists

Member of the National Scholars Honor Society

Member of Golden Key International Honor Society TTU Chapter

Member of Honor Society of Agriculture Gamma Sigma Delta TTU Chapter

RB Shrestha Page 9

January 26 2014

9

Member of American Association for Advancement in Science

Founder Member of Nepalese Student Association Texas Tech University

Professional Development Trainings and Workshops

In addition to my formal academic courses I have learned various important skills through

thirteen professional development trainings and workshops The training includes grant

writing training computer programing (R and Python) Integrated Pest Management (IPM)

training Training of Trainers (TOT) and plant biotechnology training Immuno-marking and

Enzyme Linked Immuno Sorbent (ELISA) training microsatellite DNA marker discovery

training teaching enhancement (Blackboard and WebCT) training laboratory safety (handling

radioactive materials pesticide application and shipment of hazardous materials) Compliance

training for conducting regulated Monsanto cotton field trial and training on cotton growth and

development monitoring using COTMAN software

I have also participated in short training courses in professional development such as time

management supervision project management problem solving conflict management scientific

methods information security awareness work place ethics creating discrimination free work

spaces and reporting fraud waste and abuse

Computational or Quantitative Skills and Experience

Through formal course work short training work experience and self-learning exercises I have

acquired computer skills on the application of 29 different computer software and programs

and used them in various research projects My computational skills include statistical data

analysis using SAS SPSS MATLAB SigmaPlot and ArcGIS online course preparation and

teaching using Blackboard and WebCT integrated reference management using EndNote

Medley and Reference Manager documentation database management and technical

communication using Microsoft Office Word Excel Access PowerPoint and Outlook

population genetics and phylogenetic data analysis using PAUP PHYLIP ARLEQUIN

STRUCTURE GenAlEx NTSYS PopGen MEGA AFLP SURV and TotalLab publishing and

graphic design using Adobe Photoshop Illustrator and Paint

RB Shrestha Page 10

January 26 2014

10

Publications and Presentations

1 Peer Reviewed Journal Articles

(15 totals 150 times citied) web httpscholargooglecomcitationsuser=JphkwaMAAAAJamphl=en

11 Shrestha R B and M N Parajulee 2013 Potential cotton aphid Aphis gossypii

population suppression by arthropod predators in upland cotton Journal of Insect Science

20(6)778-788

12 Parajulee M N R B Shrestha J E Slosser and D G Bordovsky 2011 Effects of

skip-row planting pattern and planting date on dryland cotton insect pest abundance and

selected plant parameters Southwestern Entomologist 36 21-39

13 Shrestha R B and M N Parajulee 2010 Effect of tillage and planting date on seasonal

abundance and diversity of predacious ground beetles in cotton Journal of Insect Science

10 (174) 1-16

14 Matis T I M N Parajulee J H Matis and R B Shrestha 2008 A mechanistic model

based analysis of cotton aphid population dynamics data Journal of Agriculture and

Forestry Ecology 10 355-362

15 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Egyptian Journal of Agricultural Research 86 57-81

16 Shrestha R B M N Parajulee O P Perera B E Scheffler and L D Densmore

2007 Characterization of microsatellite loci in the western tarnished plant bug Lygus

hesperus (Knight) (Hemiptera Miridae) Molecular Ecology Notes 7 1342-1344

17 Parajulee M N R B Shrestha and J F Leser 2006 Influence of tillage planting date

and Bt cultivar on seasonal abundance and within-plant distribution patterns of thrips and

cotton fleahoppers in cotton International Journal of Pest Management 52 249-260

18 Parajulee M N R B Shrestha and J F Leser 2006 Dispersion patterns and fixed

precision sequential sampling plans for western flower thrips (Thysanoptera Thripidae)

and cotton fleahoppers (Hemiptera Miridae) in cotton Journal of Economic Entomology

99 568-577

19 Parajulee M N R B Shrestha J F Leser D B Wester and C Blanco 2006

Evaluation of the functional response of selected cotton arthropod predators on bollworm

eggs in the laboratory and effect of temperature on their predation efficiency

Environmental Entomology 35 379-386

110 Chen F G Wu F Ge M N Parajulee and R B Shrestha 2005 Effects of elevated

CO2 and transgenic Bt cotton on plant chemistry performance and feeding of an insect

herbivore the cotton bollworm Entomologia Experimentalis et Applicata 115 341-350

RB Shrestha Page 11

January 26 2014

11

111 Dahal G R B Shrestha and R B Thapa 1997 Species composition and relative

abundance of rice green leafhopper (Nephotettix spp) in different altitude regions of

Nepal International Journal of Pest Management 43(1) 49-58

112 Dahal G A Druka T M Burns L C Villegas Z Fan R B Shrestha and R Hull

1996 Some biological and genomic properties of rice tungro bacilliform badna virus and

rice tungro spherical waika virus from Nepal Annals of Applied Biology 129 (2) 267-

287

113 Pokharel R R G Dahal and R B Shrestha 1994 Association of nematode

populations and virus-like symptoms in papaya Journal of Institute of Agriculture and

Animal Science 15 107-113

114 Dahal G R B Shrestha N K Khatri Z Fan and R Hull 1993 Incidence of virus

diseases of rice in Nepal Journal of Institute of Agriculture and Animal science 14 115-

116

115 Dahal G R R Pokharel and R B Shrestha 1992 A bibliographic database of plant

pathology in Nepal Journal of Institute of Agriculture and Animal Science 13 53-64

2 Papers Published in Refereed Proceedings (2 Total)

21 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) Proceedings World Cotton Research

Conference (CD)

22 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review pp 154 -167

Proceeding of second National Conference of Science and Technology

3 Papers in preparation for submission in Journals (5 total)

31 Shrestha R B S R K Jakka and A J Gassmann 2014 Survivorship of Western Corn

Rootworm problem field population on Bt corn hybrids revealed cross resistance between

Cry3Bb and mCry3A Journal of Economic Entomology (In preparation)

32 Shrestha R B S R K Jakka and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing eCry3A mCry3A

and eCrymCry3A stacked genes Journal of Economic Entomology (In preparation)

33 Jakka S R K R B Shrestha and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing Cry3Bb and

Cry3435Ab Journal of Economic Entomology (In preparation)

RB Shrestha Page 12

January 26 2014

12

34 Shrestha R B M N Parajulee S C Carroll J K Dever M D Arnold and J R

Hagler 2014 Characterization of the intercrop movement behavior of Lygus hesperus

between cotton and alfalfa Environmental Entomology (In preparation)

35 Shrestha R B M N Parajulee L Densmore and M San Francisco 2014

Microsatellite marker based genetic diversity of Lygus hesperus from Texas High Plains

Journal of Insect Science (In preparation)

4 Papers Published in Non-Refereed Proceedings (35 Total)

41 R B Shrestha M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

42 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

43 Parajulee M N R B Shrestha and S C Carroll 2013 Evaluation of organic pesticides

for western flower thrips management in seedling cotton Effect on plant parameters

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

44 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

45 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

46 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

47 M N Parajulee Shrestha R B W O McSpadden and SC Carroll 2011

Development of metapopulation approach for landscape-level Lygus Hesperus

management in Texas pp 220 In Proceedings of World Cotton Research Conference-5

Nov7-11 2011 Mumbai India

48 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2011 Evaluation

of varietal differences in COTMAN compensation capacity values in high-input cotton

subjected to varying levels of Lygus-induced fruit damage pp 825-829 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

RB Shrestha Page 13

January 26 2014

13

49 Parajulee M N M B Adhikari D L Kerns R B Shrestha and S C Carroll 2011

Insecticide termination rule for western tarnished plant bug in the Texas High Plains pp

1204-1208 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

410 Parajulee M N D Neupane R B Shrestha Y Yang and L T Wilson 2011 Effect

of cotton fleahopper density on pre-flower fruit loss and plant biomass in cotton pp 1095-

1099 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

411 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on Lygus hesperus abundance in adjacent cotton field A field marking study pp

853-858 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

412 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains pp 1180-88 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

413 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton pp 1155-1162 Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

414 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton pp 1163-1169 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

415 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults versus nymphs pp 849-852 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

416 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa pp 873-881

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

417 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions pp 1159-1162 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

418 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris pp 1118-1129

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

419 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip irrigated cotton pp 1251-

RB Shrestha Page 14

January 26 2014

14

1256 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

420 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus bugs from

Texas pp 1693-1699 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

421 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains pp 1700-1703

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

422 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus pp 1443-1446 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

423 Albeldantildeo W A J E Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity pp 1495-1497 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

424 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton pp 1767-1773 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

425 Parajulee M N R B Shrestha S C Carroll P L Bommireddy A M Cranmer and

K F Bronson 2005 Variable rate of irrigation water and nitrogen Potential of site-

specific management of cotton aphids pp 1735-1742 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

426 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton pp 1779-1783 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

427 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

cotton arthropod predators to bollworm eggs in the laboratory pp 1652-1659

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

428 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii p 387

Proceedings 15th

International Plant Protection Congress Foreign Language Press

Beijing China

429 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research pp 1918-1922 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

430 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Seasonal abundance patterns of thrips and fleahoppers in Texas High

RB Shrestha Page 15

January 26 2014

15

Plains cotton pp 1375-1380 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

431 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains pp 970-973 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

432 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetle in the laboratory pp 1060-1063 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

433 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in Texas High Plains pp 1370-1371 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

434 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed pp 1537-1539 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

435 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton pp 1372-1374 Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

5 Published Scientific Reports (23 Total)

51 Parajulee M N S C Carroll R B Shrestha J P Bordovsky 2012 Effect of nitrogen

fertilizer on cotton host-plant quality and its impact on arthropod activity Helms TX

2012

52 Parajulee M N R B Shrestha S Carroll and Wayne Keeling 2012 Cotton yield

response to late-season Lygus plant bug infestations as influenced by cultivar x irrigation

level treatments Lamesa TX 2012

53 Parajulee M N R B Shrestha and S C Carroll 2012 Characterization of Cotton Crop

Response to Thrips Injury for Improved Thrips Management in Texas High Plains Cotton

2012 Annual Report Cotton Incorporated Core Program Project Number 12-364

54 Parajulee M N R B Shrestha and S Carroll 2012 Development of Economic

Threshold and Management Recommendations for Lygus in Texas High Plains Cotton

2012 Annual Report (Year 1) Cotton IncorporatedTexas State Support Program Project

Number 12-112TX

55 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluating varietal

difference in COTMAN compensation capacity value in drip irrigated cotton using Lygus-

RB Shrestha Page 16

January 26 2014

16

induced fruit damage 2011 Final report Cotton Incorporated Core Program Project

Number 09-596 pp 1-16

56 Parajulee M N S C Carroll R BShrestha AM Cranmer and J P Bordovsky 2011

Influence of soil nitrogen level on seasonal activity of cotton arthropods and lint yield

under drip irrigation (field 6h) 2011 Helms Research Farm Annual Report p19

57 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluation of

westerntarnished plant bug as a late season pest of cotton 2011 Final report Cotton

IncorporatedState Support Program Project Number 08-305TX pp 1-18

58 Parajulee M N O McSpadden R B Shrestha S Carroll and W Keeling 2011

Cotton fruitingyield compensation after Lygus induced square loss as influenced by

variety x water treatments Lamesa TX 2011 Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 30-31

59 Parajulee M N B Kesey O McSpadden S Carroll R B Shrestha and W Keeling

2010 Effect of cover crop on arthropod population dynamics in subsurface drip irrigated

cotton at AG-CARES Lamesa TX 2010 Agricultural Complex for Advanced Research

and Extension Systems Annual Report pp45-46

510 Parajulee M N O McSpadden D Neupane R B Shrestha S Carroll and W

Keeling 2010 Cotton fruitingyield compensation after Lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2010 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 47-48

511 Parajulee M N R B Shrestha O McSpadden A Bastola D Neupane S Carroll and

W Keeling 2009 Cotton fruitingyield compensation after lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2009 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 46-47

512 Parajulee M N S C Carroll R BShrestha R J Kesey D M Nesmith and J P

Bordovsky 2009 Influence of soil nitrogen level on seasonal activity of cotton arthropods

and lint yield under drip irrigation 2009 Helms Research Farm Annual Report p18

513 Parajulee M N A Bastola R B Shrestha and W Keeling 2009 Evaluation of cotton

boll susceptibility to Lygus bugs as influenced by cultivar x irrigation interaction

AGCARES Lamesa TX 2009Agricultural Complex for Advanced Research and

Extension Systems Annual Report pp 48-49

514 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2004 Influence of soil nitrogen level on seasonal activity of cotton arthropods in drip

irrigation system Helms Research Farm Annual Report p 25

515 Parajulee M N J F Leser R B Shrestha A K Barman and R K Boman 2004

Evaluating the merits of sub-threshold effects of the Bollgard technology on the

economics of bollworm management Helms Research Farm Annual Report p 14

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 4: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 4

January 26 2014

4

2012 CO-PI Cotton Incorporated - Texas State Support Committee competitive grant

(Characterization of Cotton Crop Response to Thrips Injury for Improved Thrips

Management in Texas High Plains Cotton) $27000 per year (2012-2014 funded

annually)

2012 CO-PI Cotton Incorporated - Texas State Support Committee competitive grant

(Development of Economic Threshold and Management Recommendations for Lygus

in Texas High Plains Cotton) $30000 per year (2012-2014 funded annually)

2010 CO-PI International Cotton Research Center competitive grant (Lygus Bugs

Investigation of Predisposition Factors Causing Outbreaks in Order to Strengthen

Cotton Pest Management Strategies) $65000 (2010-2011)

2010 Travel grant from Biological Science Department and Graduate School TTU for

participation at 58th

annual meeting of ESA San Diego CA (Dec 12-15) ($800)

2009 Travel grant from Biological Science Department and Graduate School TTU for

participation and presentation at 57th

annual meeting of ESA Indianapolis IN (Dec

13-16) ($1000)

2009 Third place oral presentation award for student competition at Entomological Society

of America Southwestern Branch Meeting Stillwater OK (Feb 23-26)

2008 Travel grant from Biological Science Department and Graduate School for

participation at 56th

annual meeting of ESA Reno NV (Nov 16-19) ($1000)

2007 Travel grant from Biological Science Department and Graduate School TTU for

participation at 55th

annual meeting of ESA San Diego CA (Dec 9-12) ($700)

2006 Travel grant from Biological Science Department and Graduate School TTU for

participation at 54th

annual meeting of ESA Indianapolis IN (Dec 10-13) ($700)

2003 West Texas Agricultural Chemicals Institute ($1000 scholarship award)

2003 Leland Chandler Endowed Scholarship from Graduate School TTU ($500)

2003 Harold and Mary Dregne Graduate Scholarship from Graduate School TTU ($500)

2001 Netherlands Fellowship Program International Training Course on IPM in IAC

Wageningen the Netherlands (March 18-June 30)

2000 UNDP-IPM Training Fellowship International Training Course on crop protection in

CICAT Guangzhou China (May 5 to July 4)

1998 FAO IPM project in rice Trainers Training Fellowship Trainers Training on IPM in

rice in Dhanusa Nepal (April 6 to July 10)

1993 USAIDNepal Diploma in Biotechnology Training Fellowship Laboratory Training

on Plant Biotechnology in RLABB Kathmandu Nepal (Feb 13-May 13)

RB Shrestha Page 5

January 26 2014

5

Peer Review Service for Entomology Journals

I have reviewed many scientific papers for following entomological journals

Journal of Insect Science

Journal of Economic Entomology

Environmental Entomology

Neotropical Entomology

Entomologia Experimentalis et Applicata

Extension Experience in the USA

During my graduate programs (Master and PhD) from 2002 to 2011 I worked as graduate

research assistant in Texas AampM AgriLife Research and Extension Center Lubbock During this

period I got opportunity to interact closely with many extension specialists and research scientists

IPM and county agriculture extension agents growers crop consultants industry representatives

university professors and USDA scientists I have participated in many agricultural extension

activities Dr Jim Leser (Professor Extension Entomology in Texas AampM) was my masters

graduate advisory committee member I have closely worked with Dr David Kerns (Associate

Professor Extension Entomology in Texas AampM) in various collaborative extension projects

Below are the lists of some of my extension experience gained in Lubbock Texas

Participated in a Corn Rootworm Field day in Boon Iowa August 8 2013

Participated and given invited presentation in 3 crop consultant meetings in Texas

Participated and given invited talks in 4 plains cotton growers and cotton incorporated

annual meetings

Participated and given invited talk in 1 county extension agents refresher training

conferences

Participated in 5 field days and turn row meetings

Participated in 4 industries annual technology update meetings

Organized 4 insect expo insect zoo in science spectrum museum

Conducted many product evaluation field trials in growers field

Attended many commodity group (plains cotton growers advisory group) meetings

Given gt80 presentations for various audience in many meeting conferences

symposium workshops

Guided 3 group of international delegates and scientist visit or tours

Given 1 invited talk in research-extension interaction science conference

RB Shrestha Page 6

January 26 2014

6

Conducted many season-long insect pest sampling or scouting in various experimental

fields for many extension faculties collaborators

Conducted product evaluation field research in collaboration of various industries

Given 1 invited guest lectures for graduate and undergraduate classes in Texas Tech

University

Collaborated with many Extension specialists (Extension entomologists Irrigation

engineers Weed scientistAgronomist Pathologists Economists Plant breeders etc) on

various extension program and field research

Collaboration with many IPM agents county extension agents crop consultants USDA

scientists University professors and many graduate undergraduate students

Collaborated with many entomology faculties from various universities

Organized and moderated symposium in national and regional levels entomology

society meetings

Taught 3 graduate and undergraduate level entomology courses in university

Delivered entomology science and career demonstrations tour and presentations to

many groups of middle school students undergraduate students and upper bound

programrsquos students

Extension Experience in Nepal

I was a Crop Protection Extension Officer in the Department of Agriculture Nepal for 8 years

(1994-2002) I was the team leader of crop protection Extension programs in the district The goal

of my crop protection program was to disseminate modern crop protection technologies to

growers As a district IPM coordinator and crop protection officers I had managed district level

IPM program with the help of 15 junior field technicians and technical associates Examples of

extension activities conducted are as follows

Training and talks gt400 in-field training sessions at grower meetings

Field product evaluation and result demonstrations gt400 crop protection

demonstrations

Mass communications Conducted a radio talk program on rice blast disease and two

TV talk programs on pest management in mushroom cultivation in Nepal

Field days Organized four farmersrsquo field day

Farmersrsquo field schools Conducted three IPM farmersrsquo field schools in rice and cole

crops

Crop disease and insect pest diagnostic service

Pesticide regulation implementation Activity included pesticide registration resellers

training and pesticide inspection

Crop protection program planning monitoring and evaluation (regional and national)

RB Shrestha Page 7

January 26 2014

7

Postharvest loss management program such as grain storage improvement zero-energy

potato storage and cellar fruit storage

Training of junior agriculture technicians and progressive lead farmers

Distribution of posters leaflets booklets pamphlets and calendars

Crop protection training kits and subsidies distribution

Collaboration with research scientists in National Agriculture Research Council for

technology update and feedback on new crop protection technologies

Annual progress report publication (1995-2001)

Growers competition and award program

Collaborative Team Work Experiences

I have worked in close collaboration with several scientists and people from other national and

international laboratories and have published scientific papers coauthored with more than 30

people from different institutions

Leadership and Service to Professional Societies

Contributions to Professional Societies

2013 ESA Annual Meeting Member Symposium organizer Austin TX (November 10-13)

2013 ESA Annual Meeting Graduate Student Ten-Minute Paper competition session

moderator Austin TX (November 10-13) P-IE Section

Monday November 11 2013 800 AM-1200 PM

2012-2013 President Society of Overseas Nepalese Entomologists (SONE)

2012 ESA Annual Meeting Member Symposium organizer Knoxville TN (Nov 11-14)

2012 ESA Annual Meeting Symposium moderator Knoxville TN (November 11-14) P-IE

Section Environmental Entomology Symposium Tuesday November 13 2012 800

AM-1215 PM

2011-2012 Vice president Society of Overseas Nepalese Entomologists (SONE)

2011 ESA Annual Meeting Student Oral Competition Judge Reno NV (November 13-16)

2010 ESA Annual Meeting Symposium Organizer and Moderator San Diego CA

(December 12-16)

2010 ESA Annual Meeting Student Volunteer San Diego CA (December 12-16)

2009 ESA-SWB Annual Meeting Student Volunteer Stillwater OK (February 23-26)

2009 ESA-SWB Student Affairs Committee Member

2009 ESA Annual Meeting Student Volunteer Indianapolis IN (December 12-16)

RB Shrestha Page 8

January 26 2014

8

2009 ESA Annual Meeting Student Poster Competition Judge Indianapolis IN (December

12-16)

2008 ESA Annual Meeting Student Volunteer Reno NV (December 12-16)

2006 ESA-SWB Annual Meeting Student Volunteer Austin TX (February 28-March 2)

2006 ESA-SWB Annual Meeting Student Poster Competition Judge Austin TX (February

28-March 2)

2004 ESA-SWB Annual Meeting Student Volunteer Lubbock TX (February 23-26)

2004 ESA-SWB Annual Meeting Student Affairs Committee Member Lubbock TX

(February 23-26)

Conference Symposium Management

2014 Shrestha R B S R K Jakka and A J Gassmann 2014 co-organizing a symposium

(proposal accepted) in ESA NCB Annual Meeting Proposed title Advancements and

Challenges in Western Corn Rootworm Management Des Moines IA (March 9-10

2014)

2013 Shrestha R B B K Gautam J Rijal and S Gautam 2013 Jointly organized and

moderated Member symposium on ldquoPromoting Entomological Collaboration through

Inter-societal Network and Information Sharingrdquo for the 61th

annual meeting of ESA

Austin TX (November 10-13) Tuesday November 12 2013 600 PM-1000 PM

2012 Shrestha R B and R R Pandey 2012 Jointly organized and moderated Member

symposium on ldquoGlobal Collaboration for the Development of Next Generation IPM

Programrdquo for the 60th

annual meeting of ESA Knoxville TN (November 11-14)

Wednesday November 14 2012 800 AM-1200 PM

2010 Shrestha R B and M N Parajulee 2010 Jointly organized and moderated Member

symposium on ldquoMolecular and Other Novel Tools in Ecological Researchrdquo for the 58th

annual meeting of ESA San Diego CA (December 12-15) Wednesday December 15

2010 115 PM-330 PM

Active Memberships in Professional and Honor Societies

Member of Entomological Society of America

Member of Ecological Society of America

Member of Society of Southwestern Entomologists

Member of High Plains Association of Crop Consultants

President of Society of Overseas Nepalese Entomologists

Member of the National Scholars Honor Society

Member of Golden Key International Honor Society TTU Chapter

Member of Honor Society of Agriculture Gamma Sigma Delta TTU Chapter

RB Shrestha Page 9

January 26 2014

9

Member of American Association for Advancement in Science

Founder Member of Nepalese Student Association Texas Tech University

Professional Development Trainings and Workshops

In addition to my formal academic courses I have learned various important skills through

thirteen professional development trainings and workshops The training includes grant

writing training computer programing (R and Python) Integrated Pest Management (IPM)

training Training of Trainers (TOT) and plant biotechnology training Immuno-marking and

Enzyme Linked Immuno Sorbent (ELISA) training microsatellite DNA marker discovery

training teaching enhancement (Blackboard and WebCT) training laboratory safety (handling

radioactive materials pesticide application and shipment of hazardous materials) Compliance

training for conducting regulated Monsanto cotton field trial and training on cotton growth and

development monitoring using COTMAN software

I have also participated in short training courses in professional development such as time

management supervision project management problem solving conflict management scientific

methods information security awareness work place ethics creating discrimination free work

spaces and reporting fraud waste and abuse

Computational or Quantitative Skills and Experience

Through formal course work short training work experience and self-learning exercises I have

acquired computer skills on the application of 29 different computer software and programs

and used them in various research projects My computational skills include statistical data

analysis using SAS SPSS MATLAB SigmaPlot and ArcGIS online course preparation and

teaching using Blackboard and WebCT integrated reference management using EndNote

Medley and Reference Manager documentation database management and technical

communication using Microsoft Office Word Excel Access PowerPoint and Outlook

population genetics and phylogenetic data analysis using PAUP PHYLIP ARLEQUIN

STRUCTURE GenAlEx NTSYS PopGen MEGA AFLP SURV and TotalLab publishing and

graphic design using Adobe Photoshop Illustrator and Paint

RB Shrestha Page 10

January 26 2014

10

Publications and Presentations

1 Peer Reviewed Journal Articles

(15 totals 150 times citied) web httpscholargooglecomcitationsuser=JphkwaMAAAAJamphl=en

11 Shrestha R B and M N Parajulee 2013 Potential cotton aphid Aphis gossypii

population suppression by arthropod predators in upland cotton Journal of Insect Science

20(6)778-788

12 Parajulee M N R B Shrestha J E Slosser and D G Bordovsky 2011 Effects of

skip-row planting pattern and planting date on dryland cotton insect pest abundance and

selected plant parameters Southwestern Entomologist 36 21-39

13 Shrestha R B and M N Parajulee 2010 Effect of tillage and planting date on seasonal

abundance and diversity of predacious ground beetles in cotton Journal of Insect Science

10 (174) 1-16

14 Matis T I M N Parajulee J H Matis and R B Shrestha 2008 A mechanistic model

based analysis of cotton aphid population dynamics data Journal of Agriculture and

Forestry Ecology 10 355-362

15 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Egyptian Journal of Agricultural Research 86 57-81

16 Shrestha R B M N Parajulee O P Perera B E Scheffler and L D Densmore

2007 Characterization of microsatellite loci in the western tarnished plant bug Lygus

hesperus (Knight) (Hemiptera Miridae) Molecular Ecology Notes 7 1342-1344

17 Parajulee M N R B Shrestha and J F Leser 2006 Influence of tillage planting date

and Bt cultivar on seasonal abundance and within-plant distribution patterns of thrips and

cotton fleahoppers in cotton International Journal of Pest Management 52 249-260

18 Parajulee M N R B Shrestha and J F Leser 2006 Dispersion patterns and fixed

precision sequential sampling plans for western flower thrips (Thysanoptera Thripidae)

and cotton fleahoppers (Hemiptera Miridae) in cotton Journal of Economic Entomology

99 568-577

19 Parajulee M N R B Shrestha J F Leser D B Wester and C Blanco 2006

Evaluation of the functional response of selected cotton arthropod predators on bollworm

eggs in the laboratory and effect of temperature on their predation efficiency

Environmental Entomology 35 379-386

110 Chen F G Wu F Ge M N Parajulee and R B Shrestha 2005 Effects of elevated

CO2 and transgenic Bt cotton on plant chemistry performance and feeding of an insect

herbivore the cotton bollworm Entomologia Experimentalis et Applicata 115 341-350

RB Shrestha Page 11

January 26 2014

11

111 Dahal G R B Shrestha and R B Thapa 1997 Species composition and relative

abundance of rice green leafhopper (Nephotettix spp) in different altitude regions of

Nepal International Journal of Pest Management 43(1) 49-58

112 Dahal G A Druka T M Burns L C Villegas Z Fan R B Shrestha and R Hull

1996 Some biological and genomic properties of rice tungro bacilliform badna virus and

rice tungro spherical waika virus from Nepal Annals of Applied Biology 129 (2) 267-

287

113 Pokharel R R G Dahal and R B Shrestha 1994 Association of nematode

populations and virus-like symptoms in papaya Journal of Institute of Agriculture and

Animal Science 15 107-113

114 Dahal G R B Shrestha N K Khatri Z Fan and R Hull 1993 Incidence of virus

diseases of rice in Nepal Journal of Institute of Agriculture and Animal science 14 115-

116

115 Dahal G R R Pokharel and R B Shrestha 1992 A bibliographic database of plant

pathology in Nepal Journal of Institute of Agriculture and Animal Science 13 53-64

2 Papers Published in Refereed Proceedings (2 Total)

21 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) Proceedings World Cotton Research

Conference (CD)

22 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review pp 154 -167

Proceeding of second National Conference of Science and Technology

3 Papers in preparation for submission in Journals (5 total)

31 Shrestha R B S R K Jakka and A J Gassmann 2014 Survivorship of Western Corn

Rootworm problem field population on Bt corn hybrids revealed cross resistance between

Cry3Bb and mCry3A Journal of Economic Entomology (In preparation)

32 Shrestha R B S R K Jakka and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing eCry3A mCry3A

and eCrymCry3A stacked genes Journal of Economic Entomology (In preparation)

33 Jakka S R K R B Shrestha and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing Cry3Bb and

Cry3435Ab Journal of Economic Entomology (In preparation)

RB Shrestha Page 12

January 26 2014

12

34 Shrestha R B M N Parajulee S C Carroll J K Dever M D Arnold and J R

Hagler 2014 Characterization of the intercrop movement behavior of Lygus hesperus

between cotton and alfalfa Environmental Entomology (In preparation)

35 Shrestha R B M N Parajulee L Densmore and M San Francisco 2014

Microsatellite marker based genetic diversity of Lygus hesperus from Texas High Plains

Journal of Insect Science (In preparation)

4 Papers Published in Non-Refereed Proceedings (35 Total)

41 R B Shrestha M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

42 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

43 Parajulee M N R B Shrestha and S C Carroll 2013 Evaluation of organic pesticides

for western flower thrips management in seedling cotton Effect on plant parameters

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

44 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

45 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

46 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

47 M N Parajulee Shrestha R B W O McSpadden and SC Carroll 2011

Development of metapopulation approach for landscape-level Lygus Hesperus

management in Texas pp 220 In Proceedings of World Cotton Research Conference-5

Nov7-11 2011 Mumbai India

48 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2011 Evaluation

of varietal differences in COTMAN compensation capacity values in high-input cotton

subjected to varying levels of Lygus-induced fruit damage pp 825-829 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

RB Shrestha Page 13

January 26 2014

13

49 Parajulee M N M B Adhikari D L Kerns R B Shrestha and S C Carroll 2011

Insecticide termination rule for western tarnished plant bug in the Texas High Plains pp

1204-1208 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

410 Parajulee M N D Neupane R B Shrestha Y Yang and L T Wilson 2011 Effect

of cotton fleahopper density on pre-flower fruit loss and plant biomass in cotton pp 1095-

1099 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

411 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on Lygus hesperus abundance in adjacent cotton field A field marking study pp

853-858 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

412 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains pp 1180-88 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

413 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton pp 1155-1162 Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

414 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton pp 1163-1169 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

415 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults versus nymphs pp 849-852 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

416 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa pp 873-881

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

417 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions pp 1159-1162 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

418 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris pp 1118-1129

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

419 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip irrigated cotton pp 1251-

RB Shrestha Page 14

January 26 2014

14

1256 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

420 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus bugs from

Texas pp 1693-1699 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

421 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains pp 1700-1703

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

422 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus pp 1443-1446 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

423 Albeldantildeo W A J E Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity pp 1495-1497 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

424 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton pp 1767-1773 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

425 Parajulee M N R B Shrestha S C Carroll P L Bommireddy A M Cranmer and

K F Bronson 2005 Variable rate of irrigation water and nitrogen Potential of site-

specific management of cotton aphids pp 1735-1742 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

426 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton pp 1779-1783 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

427 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

cotton arthropod predators to bollworm eggs in the laboratory pp 1652-1659

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

428 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii p 387

Proceedings 15th

International Plant Protection Congress Foreign Language Press

Beijing China

429 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research pp 1918-1922 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

430 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Seasonal abundance patterns of thrips and fleahoppers in Texas High

RB Shrestha Page 15

January 26 2014

15

Plains cotton pp 1375-1380 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

431 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains pp 970-973 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

432 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetle in the laboratory pp 1060-1063 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

433 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in Texas High Plains pp 1370-1371 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

434 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed pp 1537-1539 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

435 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton pp 1372-1374 Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

5 Published Scientific Reports (23 Total)

51 Parajulee M N S C Carroll R B Shrestha J P Bordovsky 2012 Effect of nitrogen

fertilizer on cotton host-plant quality and its impact on arthropod activity Helms TX

2012

52 Parajulee M N R B Shrestha S Carroll and Wayne Keeling 2012 Cotton yield

response to late-season Lygus plant bug infestations as influenced by cultivar x irrigation

level treatments Lamesa TX 2012

53 Parajulee M N R B Shrestha and S C Carroll 2012 Characterization of Cotton Crop

Response to Thrips Injury for Improved Thrips Management in Texas High Plains Cotton

2012 Annual Report Cotton Incorporated Core Program Project Number 12-364

54 Parajulee M N R B Shrestha and S Carroll 2012 Development of Economic

Threshold and Management Recommendations for Lygus in Texas High Plains Cotton

2012 Annual Report (Year 1) Cotton IncorporatedTexas State Support Program Project

Number 12-112TX

55 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluating varietal

difference in COTMAN compensation capacity value in drip irrigated cotton using Lygus-

RB Shrestha Page 16

January 26 2014

16

induced fruit damage 2011 Final report Cotton Incorporated Core Program Project

Number 09-596 pp 1-16

56 Parajulee M N S C Carroll R BShrestha AM Cranmer and J P Bordovsky 2011

Influence of soil nitrogen level on seasonal activity of cotton arthropods and lint yield

under drip irrigation (field 6h) 2011 Helms Research Farm Annual Report p19

57 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluation of

westerntarnished plant bug as a late season pest of cotton 2011 Final report Cotton

IncorporatedState Support Program Project Number 08-305TX pp 1-18

58 Parajulee M N O McSpadden R B Shrestha S Carroll and W Keeling 2011

Cotton fruitingyield compensation after Lygus induced square loss as influenced by

variety x water treatments Lamesa TX 2011 Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 30-31

59 Parajulee M N B Kesey O McSpadden S Carroll R B Shrestha and W Keeling

2010 Effect of cover crop on arthropod population dynamics in subsurface drip irrigated

cotton at AG-CARES Lamesa TX 2010 Agricultural Complex for Advanced Research

and Extension Systems Annual Report pp45-46

510 Parajulee M N O McSpadden D Neupane R B Shrestha S Carroll and W

Keeling 2010 Cotton fruitingyield compensation after Lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2010 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 47-48

511 Parajulee M N R B Shrestha O McSpadden A Bastola D Neupane S Carroll and

W Keeling 2009 Cotton fruitingyield compensation after lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2009 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 46-47

512 Parajulee M N S C Carroll R BShrestha R J Kesey D M Nesmith and J P

Bordovsky 2009 Influence of soil nitrogen level on seasonal activity of cotton arthropods

and lint yield under drip irrigation 2009 Helms Research Farm Annual Report p18

513 Parajulee M N A Bastola R B Shrestha and W Keeling 2009 Evaluation of cotton

boll susceptibility to Lygus bugs as influenced by cultivar x irrigation interaction

AGCARES Lamesa TX 2009Agricultural Complex for Advanced Research and

Extension Systems Annual Report pp 48-49

514 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2004 Influence of soil nitrogen level on seasonal activity of cotton arthropods in drip

irrigation system Helms Research Farm Annual Report p 25

515 Parajulee M N J F Leser R B Shrestha A K Barman and R K Boman 2004

Evaluating the merits of sub-threshold effects of the Bollgard technology on the

economics of bollworm management Helms Research Farm Annual Report p 14

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 5: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 5

January 26 2014

5

Peer Review Service for Entomology Journals

I have reviewed many scientific papers for following entomological journals

Journal of Insect Science

Journal of Economic Entomology

Environmental Entomology

Neotropical Entomology

Entomologia Experimentalis et Applicata

Extension Experience in the USA

During my graduate programs (Master and PhD) from 2002 to 2011 I worked as graduate

research assistant in Texas AampM AgriLife Research and Extension Center Lubbock During this

period I got opportunity to interact closely with many extension specialists and research scientists

IPM and county agriculture extension agents growers crop consultants industry representatives

university professors and USDA scientists I have participated in many agricultural extension

activities Dr Jim Leser (Professor Extension Entomology in Texas AampM) was my masters

graduate advisory committee member I have closely worked with Dr David Kerns (Associate

Professor Extension Entomology in Texas AampM) in various collaborative extension projects

Below are the lists of some of my extension experience gained in Lubbock Texas

Participated in a Corn Rootworm Field day in Boon Iowa August 8 2013

Participated and given invited presentation in 3 crop consultant meetings in Texas

Participated and given invited talks in 4 plains cotton growers and cotton incorporated

annual meetings

Participated and given invited talk in 1 county extension agents refresher training

conferences

Participated in 5 field days and turn row meetings

Participated in 4 industries annual technology update meetings

Organized 4 insect expo insect zoo in science spectrum museum

Conducted many product evaluation field trials in growers field

Attended many commodity group (plains cotton growers advisory group) meetings

Given gt80 presentations for various audience in many meeting conferences

symposium workshops

Guided 3 group of international delegates and scientist visit or tours

Given 1 invited talk in research-extension interaction science conference

RB Shrestha Page 6

January 26 2014

6

Conducted many season-long insect pest sampling or scouting in various experimental

fields for many extension faculties collaborators

Conducted product evaluation field research in collaboration of various industries

Given 1 invited guest lectures for graduate and undergraduate classes in Texas Tech

University

Collaborated with many Extension specialists (Extension entomologists Irrigation

engineers Weed scientistAgronomist Pathologists Economists Plant breeders etc) on

various extension program and field research

Collaboration with many IPM agents county extension agents crop consultants USDA

scientists University professors and many graduate undergraduate students

Collaborated with many entomology faculties from various universities

Organized and moderated symposium in national and regional levels entomology

society meetings

Taught 3 graduate and undergraduate level entomology courses in university

Delivered entomology science and career demonstrations tour and presentations to

many groups of middle school students undergraduate students and upper bound

programrsquos students

Extension Experience in Nepal

I was a Crop Protection Extension Officer in the Department of Agriculture Nepal for 8 years

(1994-2002) I was the team leader of crop protection Extension programs in the district The goal

of my crop protection program was to disseminate modern crop protection technologies to

growers As a district IPM coordinator and crop protection officers I had managed district level

IPM program with the help of 15 junior field technicians and technical associates Examples of

extension activities conducted are as follows

Training and talks gt400 in-field training sessions at grower meetings

Field product evaluation and result demonstrations gt400 crop protection

demonstrations

Mass communications Conducted a radio talk program on rice blast disease and two

TV talk programs on pest management in mushroom cultivation in Nepal

Field days Organized four farmersrsquo field day

Farmersrsquo field schools Conducted three IPM farmersrsquo field schools in rice and cole

crops

Crop disease and insect pest diagnostic service

Pesticide regulation implementation Activity included pesticide registration resellers

training and pesticide inspection

Crop protection program planning monitoring and evaluation (regional and national)

RB Shrestha Page 7

January 26 2014

7

Postharvest loss management program such as grain storage improvement zero-energy

potato storage and cellar fruit storage

Training of junior agriculture technicians and progressive lead farmers

Distribution of posters leaflets booklets pamphlets and calendars

Crop protection training kits and subsidies distribution

Collaboration with research scientists in National Agriculture Research Council for

technology update and feedback on new crop protection technologies

Annual progress report publication (1995-2001)

Growers competition and award program

Collaborative Team Work Experiences

I have worked in close collaboration with several scientists and people from other national and

international laboratories and have published scientific papers coauthored with more than 30

people from different institutions

Leadership and Service to Professional Societies

Contributions to Professional Societies

2013 ESA Annual Meeting Member Symposium organizer Austin TX (November 10-13)

2013 ESA Annual Meeting Graduate Student Ten-Minute Paper competition session

moderator Austin TX (November 10-13) P-IE Section

Monday November 11 2013 800 AM-1200 PM

2012-2013 President Society of Overseas Nepalese Entomologists (SONE)

2012 ESA Annual Meeting Member Symposium organizer Knoxville TN (Nov 11-14)

2012 ESA Annual Meeting Symposium moderator Knoxville TN (November 11-14) P-IE

Section Environmental Entomology Symposium Tuesday November 13 2012 800

AM-1215 PM

2011-2012 Vice president Society of Overseas Nepalese Entomologists (SONE)

2011 ESA Annual Meeting Student Oral Competition Judge Reno NV (November 13-16)

2010 ESA Annual Meeting Symposium Organizer and Moderator San Diego CA

(December 12-16)

2010 ESA Annual Meeting Student Volunteer San Diego CA (December 12-16)

2009 ESA-SWB Annual Meeting Student Volunteer Stillwater OK (February 23-26)

2009 ESA-SWB Student Affairs Committee Member

2009 ESA Annual Meeting Student Volunteer Indianapolis IN (December 12-16)

RB Shrestha Page 8

January 26 2014

8

2009 ESA Annual Meeting Student Poster Competition Judge Indianapolis IN (December

12-16)

2008 ESA Annual Meeting Student Volunteer Reno NV (December 12-16)

2006 ESA-SWB Annual Meeting Student Volunteer Austin TX (February 28-March 2)

2006 ESA-SWB Annual Meeting Student Poster Competition Judge Austin TX (February

28-March 2)

2004 ESA-SWB Annual Meeting Student Volunteer Lubbock TX (February 23-26)

2004 ESA-SWB Annual Meeting Student Affairs Committee Member Lubbock TX

(February 23-26)

Conference Symposium Management

2014 Shrestha R B S R K Jakka and A J Gassmann 2014 co-organizing a symposium

(proposal accepted) in ESA NCB Annual Meeting Proposed title Advancements and

Challenges in Western Corn Rootworm Management Des Moines IA (March 9-10

2014)

2013 Shrestha R B B K Gautam J Rijal and S Gautam 2013 Jointly organized and

moderated Member symposium on ldquoPromoting Entomological Collaboration through

Inter-societal Network and Information Sharingrdquo for the 61th

annual meeting of ESA

Austin TX (November 10-13) Tuesday November 12 2013 600 PM-1000 PM

2012 Shrestha R B and R R Pandey 2012 Jointly organized and moderated Member

symposium on ldquoGlobal Collaboration for the Development of Next Generation IPM

Programrdquo for the 60th

annual meeting of ESA Knoxville TN (November 11-14)

Wednesday November 14 2012 800 AM-1200 PM

2010 Shrestha R B and M N Parajulee 2010 Jointly organized and moderated Member

symposium on ldquoMolecular and Other Novel Tools in Ecological Researchrdquo for the 58th

annual meeting of ESA San Diego CA (December 12-15) Wednesday December 15

2010 115 PM-330 PM

Active Memberships in Professional and Honor Societies

Member of Entomological Society of America

Member of Ecological Society of America

Member of Society of Southwestern Entomologists

Member of High Plains Association of Crop Consultants

President of Society of Overseas Nepalese Entomologists

Member of the National Scholars Honor Society

Member of Golden Key International Honor Society TTU Chapter

Member of Honor Society of Agriculture Gamma Sigma Delta TTU Chapter

RB Shrestha Page 9

January 26 2014

9

Member of American Association for Advancement in Science

Founder Member of Nepalese Student Association Texas Tech University

Professional Development Trainings and Workshops

In addition to my formal academic courses I have learned various important skills through

thirteen professional development trainings and workshops The training includes grant

writing training computer programing (R and Python) Integrated Pest Management (IPM)

training Training of Trainers (TOT) and plant biotechnology training Immuno-marking and

Enzyme Linked Immuno Sorbent (ELISA) training microsatellite DNA marker discovery

training teaching enhancement (Blackboard and WebCT) training laboratory safety (handling

radioactive materials pesticide application and shipment of hazardous materials) Compliance

training for conducting regulated Monsanto cotton field trial and training on cotton growth and

development monitoring using COTMAN software

I have also participated in short training courses in professional development such as time

management supervision project management problem solving conflict management scientific

methods information security awareness work place ethics creating discrimination free work

spaces and reporting fraud waste and abuse

Computational or Quantitative Skills and Experience

Through formal course work short training work experience and self-learning exercises I have

acquired computer skills on the application of 29 different computer software and programs

and used them in various research projects My computational skills include statistical data

analysis using SAS SPSS MATLAB SigmaPlot and ArcGIS online course preparation and

teaching using Blackboard and WebCT integrated reference management using EndNote

Medley and Reference Manager documentation database management and technical

communication using Microsoft Office Word Excel Access PowerPoint and Outlook

population genetics and phylogenetic data analysis using PAUP PHYLIP ARLEQUIN

STRUCTURE GenAlEx NTSYS PopGen MEGA AFLP SURV and TotalLab publishing and

graphic design using Adobe Photoshop Illustrator and Paint

RB Shrestha Page 10

January 26 2014

10

Publications and Presentations

1 Peer Reviewed Journal Articles

(15 totals 150 times citied) web httpscholargooglecomcitationsuser=JphkwaMAAAAJamphl=en

11 Shrestha R B and M N Parajulee 2013 Potential cotton aphid Aphis gossypii

population suppression by arthropod predators in upland cotton Journal of Insect Science

20(6)778-788

12 Parajulee M N R B Shrestha J E Slosser and D G Bordovsky 2011 Effects of

skip-row planting pattern and planting date on dryland cotton insect pest abundance and

selected plant parameters Southwestern Entomologist 36 21-39

13 Shrestha R B and M N Parajulee 2010 Effect of tillage and planting date on seasonal

abundance and diversity of predacious ground beetles in cotton Journal of Insect Science

10 (174) 1-16

14 Matis T I M N Parajulee J H Matis and R B Shrestha 2008 A mechanistic model

based analysis of cotton aphid population dynamics data Journal of Agriculture and

Forestry Ecology 10 355-362

15 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Egyptian Journal of Agricultural Research 86 57-81

16 Shrestha R B M N Parajulee O P Perera B E Scheffler and L D Densmore

2007 Characterization of microsatellite loci in the western tarnished plant bug Lygus

hesperus (Knight) (Hemiptera Miridae) Molecular Ecology Notes 7 1342-1344

17 Parajulee M N R B Shrestha and J F Leser 2006 Influence of tillage planting date

and Bt cultivar on seasonal abundance and within-plant distribution patterns of thrips and

cotton fleahoppers in cotton International Journal of Pest Management 52 249-260

18 Parajulee M N R B Shrestha and J F Leser 2006 Dispersion patterns and fixed

precision sequential sampling plans for western flower thrips (Thysanoptera Thripidae)

and cotton fleahoppers (Hemiptera Miridae) in cotton Journal of Economic Entomology

99 568-577

19 Parajulee M N R B Shrestha J F Leser D B Wester and C Blanco 2006

Evaluation of the functional response of selected cotton arthropod predators on bollworm

eggs in the laboratory and effect of temperature on their predation efficiency

Environmental Entomology 35 379-386

110 Chen F G Wu F Ge M N Parajulee and R B Shrestha 2005 Effects of elevated

CO2 and transgenic Bt cotton on plant chemistry performance and feeding of an insect

herbivore the cotton bollworm Entomologia Experimentalis et Applicata 115 341-350

RB Shrestha Page 11

January 26 2014

11

111 Dahal G R B Shrestha and R B Thapa 1997 Species composition and relative

abundance of rice green leafhopper (Nephotettix spp) in different altitude regions of

Nepal International Journal of Pest Management 43(1) 49-58

112 Dahal G A Druka T M Burns L C Villegas Z Fan R B Shrestha and R Hull

1996 Some biological and genomic properties of rice tungro bacilliform badna virus and

rice tungro spherical waika virus from Nepal Annals of Applied Biology 129 (2) 267-

287

113 Pokharel R R G Dahal and R B Shrestha 1994 Association of nematode

populations and virus-like symptoms in papaya Journal of Institute of Agriculture and

Animal Science 15 107-113

114 Dahal G R B Shrestha N K Khatri Z Fan and R Hull 1993 Incidence of virus

diseases of rice in Nepal Journal of Institute of Agriculture and Animal science 14 115-

116

115 Dahal G R R Pokharel and R B Shrestha 1992 A bibliographic database of plant

pathology in Nepal Journal of Institute of Agriculture and Animal Science 13 53-64

2 Papers Published in Refereed Proceedings (2 Total)

21 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) Proceedings World Cotton Research

Conference (CD)

22 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review pp 154 -167

Proceeding of second National Conference of Science and Technology

3 Papers in preparation for submission in Journals (5 total)

31 Shrestha R B S R K Jakka and A J Gassmann 2014 Survivorship of Western Corn

Rootworm problem field population on Bt corn hybrids revealed cross resistance between

Cry3Bb and mCry3A Journal of Economic Entomology (In preparation)

32 Shrestha R B S R K Jakka and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing eCry3A mCry3A

and eCrymCry3A stacked genes Journal of Economic Entomology (In preparation)

33 Jakka S R K R B Shrestha and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing Cry3Bb and

Cry3435Ab Journal of Economic Entomology (In preparation)

RB Shrestha Page 12

January 26 2014

12

34 Shrestha R B M N Parajulee S C Carroll J K Dever M D Arnold and J R

Hagler 2014 Characterization of the intercrop movement behavior of Lygus hesperus

between cotton and alfalfa Environmental Entomology (In preparation)

35 Shrestha R B M N Parajulee L Densmore and M San Francisco 2014

Microsatellite marker based genetic diversity of Lygus hesperus from Texas High Plains

Journal of Insect Science (In preparation)

4 Papers Published in Non-Refereed Proceedings (35 Total)

41 R B Shrestha M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

42 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

43 Parajulee M N R B Shrestha and S C Carroll 2013 Evaluation of organic pesticides

for western flower thrips management in seedling cotton Effect on plant parameters

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

44 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

45 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

46 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

47 M N Parajulee Shrestha R B W O McSpadden and SC Carroll 2011

Development of metapopulation approach for landscape-level Lygus Hesperus

management in Texas pp 220 In Proceedings of World Cotton Research Conference-5

Nov7-11 2011 Mumbai India

48 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2011 Evaluation

of varietal differences in COTMAN compensation capacity values in high-input cotton

subjected to varying levels of Lygus-induced fruit damage pp 825-829 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

RB Shrestha Page 13

January 26 2014

13

49 Parajulee M N M B Adhikari D L Kerns R B Shrestha and S C Carroll 2011

Insecticide termination rule for western tarnished plant bug in the Texas High Plains pp

1204-1208 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

410 Parajulee M N D Neupane R B Shrestha Y Yang and L T Wilson 2011 Effect

of cotton fleahopper density on pre-flower fruit loss and plant biomass in cotton pp 1095-

1099 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

411 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on Lygus hesperus abundance in adjacent cotton field A field marking study pp

853-858 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

412 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains pp 1180-88 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

413 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton pp 1155-1162 Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

414 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton pp 1163-1169 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

415 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults versus nymphs pp 849-852 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

416 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa pp 873-881

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

417 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions pp 1159-1162 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

418 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris pp 1118-1129

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

419 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip irrigated cotton pp 1251-

RB Shrestha Page 14

January 26 2014

14

1256 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

420 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus bugs from

Texas pp 1693-1699 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

421 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains pp 1700-1703

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

422 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus pp 1443-1446 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

423 Albeldantildeo W A J E Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity pp 1495-1497 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

424 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton pp 1767-1773 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

425 Parajulee M N R B Shrestha S C Carroll P L Bommireddy A M Cranmer and

K F Bronson 2005 Variable rate of irrigation water and nitrogen Potential of site-

specific management of cotton aphids pp 1735-1742 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

426 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton pp 1779-1783 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

427 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

cotton arthropod predators to bollworm eggs in the laboratory pp 1652-1659

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

428 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii p 387

Proceedings 15th

International Plant Protection Congress Foreign Language Press

Beijing China

429 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research pp 1918-1922 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

430 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Seasonal abundance patterns of thrips and fleahoppers in Texas High

RB Shrestha Page 15

January 26 2014

15

Plains cotton pp 1375-1380 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

431 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains pp 970-973 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

432 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetle in the laboratory pp 1060-1063 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

433 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in Texas High Plains pp 1370-1371 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

434 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed pp 1537-1539 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

435 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton pp 1372-1374 Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

5 Published Scientific Reports (23 Total)

51 Parajulee M N S C Carroll R B Shrestha J P Bordovsky 2012 Effect of nitrogen

fertilizer on cotton host-plant quality and its impact on arthropod activity Helms TX

2012

52 Parajulee M N R B Shrestha S Carroll and Wayne Keeling 2012 Cotton yield

response to late-season Lygus plant bug infestations as influenced by cultivar x irrigation

level treatments Lamesa TX 2012

53 Parajulee M N R B Shrestha and S C Carroll 2012 Characterization of Cotton Crop

Response to Thrips Injury for Improved Thrips Management in Texas High Plains Cotton

2012 Annual Report Cotton Incorporated Core Program Project Number 12-364

54 Parajulee M N R B Shrestha and S Carroll 2012 Development of Economic

Threshold and Management Recommendations for Lygus in Texas High Plains Cotton

2012 Annual Report (Year 1) Cotton IncorporatedTexas State Support Program Project

Number 12-112TX

55 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluating varietal

difference in COTMAN compensation capacity value in drip irrigated cotton using Lygus-

RB Shrestha Page 16

January 26 2014

16

induced fruit damage 2011 Final report Cotton Incorporated Core Program Project

Number 09-596 pp 1-16

56 Parajulee M N S C Carroll R BShrestha AM Cranmer and J P Bordovsky 2011

Influence of soil nitrogen level on seasonal activity of cotton arthropods and lint yield

under drip irrigation (field 6h) 2011 Helms Research Farm Annual Report p19

57 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluation of

westerntarnished plant bug as a late season pest of cotton 2011 Final report Cotton

IncorporatedState Support Program Project Number 08-305TX pp 1-18

58 Parajulee M N O McSpadden R B Shrestha S Carroll and W Keeling 2011

Cotton fruitingyield compensation after Lygus induced square loss as influenced by

variety x water treatments Lamesa TX 2011 Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 30-31

59 Parajulee M N B Kesey O McSpadden S Carroll R B Shrestha and W Keeling

2010 Effect of cover crop on arthropod population dynamics in subsurface drip irrigated

cotton at AG-CARES Lamesa TX 2010 Agricultural Complex for Advanced Research

and Extension Systems Annual Report pp45-46

510 Parajulee M N O McSpadden D Neupane R B Shrestha S Carroll and W

Keeling 2010 Cotton fruitingyield compensation after Lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2010 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 47-48

511 Parajulee M N R B Shrestha O McSpadden A Bastola D Neupane S Carroll and

W Keeling 2009 Cotton fruitingyield compensation after lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2009 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 46-47

512 Parajulee M N S C Carroll R BShrestha R J Kesey D M Nesmith and J P

Bordovsky 2009 Influence of soil nitrogen level on seasonal activity of cotton arthropods

and lint yield under drip irrigation 2009 Helms Research Farm Annual Report p18

513 Parajulee M N A Bastola R B Shrestha and W Keeling 2009 Evaluation of cotton

boll susceptibility to Lygus bugs as influenced by cultivar x irrigation interaction

AGCARES Lamesa TX 2009Agricultural Complex for Advanced Research and

Extension Systems Annual Report pp 48-49

514 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2004 Influence of soil nitrogen level on seasonal activity of cotton arthropods in drip

irrigation system Helms Research Farm Annual Report p 25

515 Parajulee M N J F Leser R B Shrestha A K Barman and R K Boman 2004

Evaluating the merits of sub-threshold effects of the Bollgard technology on the

economics of bollworm management Helms Research Farm Annual Report p 14

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 6: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 6

January 26 2014

6

Conducted many season-long insect pest sampling or scouting in various experimental

fields for many extension faculties collaborators

Conducted product evaluation field research in collaboration of various industries

Given 1 invited guest lectures for graduate and undergraduate classes in Texas Tech

University

Collaborated with many Extension specialists (Extension entomologists Irrigation

engineers Weed scientistAgronomist Pathologists Economists Plant breeders etc) on

various extension program and field research

Collaboration with many IPM agents county extension agents crop consultants USDA

scientists University professors and many graduate undergraduate students

Collaborated with many entomology faculties from various universities

Organized and moderated symposium in national and regional levels entomology

society meetings

Taught 3 graduate and undergraduate level entomology courses in university

Delivered entomology science and career demonstrations tour and presentations to

many groups of middle school students undergraduate students and upper bound

programrsquos students

Extension Experience in Nepal

I was a Crop Protection Extension Officer in the Department of Agriculture Nepal for 8 years

(1994-2002) I was the team leader of crop protection Extension programs in the district The goal

of my crop protection program was to disseminate modern crop protection technologies to

growers As a district IPM coordinator and crop protection officers I had managed district level

IPM program with the help of 15 junior field technicians and technical associates Examples of

extension activities conducted are as follows

Training and talks gt400 in-field training sessions at grower meetings

Field product evaluation and result demonstrations gt400 crop protection

demonstrations

Mass communications Conducted a radio talk program on rice blast disease and two

TV talk programs on pest management in mushroom cultivation in Nepal

Field days Organized four farmersrsquo field day

Farmersrsquo field schools Conducted three IPM farmersrsquo field schools in rice and cole

crops

Crop disease and insect pest diagnostic service

Pesticide regulation implementation Activity included pesticide registration resellers

training and pesticide inspection

Crop protection program planning monitoring and evaluation (regional and national)

RB Shrestha Page 7

January 26 2014

7

Postharvest loss management program such as grain storage improvement zero-energy

potato storage and cellar fruit storage

Training of junior agriculture technicians and progressive lead farmers

Distribution of posters leaflets booklets pamphlets and calendars

Crop protection training kits and subsidies distribution

Collaboration with research scientists in National Agriculture Research Council for

technology update and feedback on new crop protection technologies

Annual progress report publication (1995-2001)

Growers competition and award program

Collaborative Team Work Experiences

I have worked in close collaboration with several scientists and people from other national and

international laboratories and have published scientific papers coauthored with more than 30

people from different institutions

Leadership and Service to Professional Societies

Contributions to Professional Societies

2013 ESA Annual Meeting Member Symposium organizer Austin TX (November 10-13)

2013 ESA Annual Meeting Graduate Student Ten-Minute Paper competition session

moderator Austin TX (November 10-13) P-IE Section

Monday November 11 2013 800 AM-1200 PM

2012-2013 President Society of Overseas Nepalese Entomologists (SONE)

2012 ESA Annual Meeting Member Symposium organizer Knoxville TN (Nov 11-14)

2012 ESA Annual Meeting Symposium moderator Knoxville TN (November 11-14) P-IE

Section Environmental Entomology Symposium Tuesday November 13 2012 800

AM-1215 PM

2011-2012 Vice president Society of Overseas Nepalese Entomologists (SONE)

2011 ESA Annual Meeting Student Oral Competition Judge Reno NV (November 13-16)

2010 ESA Annual Meeting Symposium Organizer and Moderator San Diego CA

(December 12-16)

2010 ESA Annual Meeting Student Volunteer San Diego CA (December 12-16)

2009 ESA-SWB Annual Meeting Student Volunteer Stillwater OK (February 23-26)

2009 ESA-SWB Student Affairs Committee Member

2009 ESA Annual Meeting Student Volunteer Indianapolis IN (December 12-16)

RB Shrestha Page 8

January 26 2014

8

2009 ESA Annual Meeting Student Poster Competition Judge Indianapolis IN (December

12-16)

2008 ESA Annual Meeting Student Volunteer Reno NV (December 12-16)

2006 ESA-SWB Annual Meeting Student Volunteer Austin TX (February 28-March 2)

2006 ESA-SWB Annual Meeting Student Poster Competition Judge Austin TX (February

28-March 2)

2004 ESA-SWB Annual Meeting Student Volunteer Lubbock TX (February 23-26)

2004 ESA-SWB Annual Meeting Student Affairs Committee Member Lubbock TX

(February 23-26)

Conference Symposium Management

2014 Shrestha R B S R K Jakka and A J Gassmann 2014 co-organizing a symposium

(proposal accepted) in ESA NCB Annual Meeting Proposed title Advancements and

Challenges in Western Corn Rootworm Management Des Moines IA (March 9-10

2014)

2013 Shrestha R B B K Gautam J Rijal and S Gautam 2013 Jointly organized and

moderated Member symposium on ldquoPromoting Entomological Collaboration through

Inter-societal Network and Information Sharingrdquo for the 61th

annual meeting of ESA

Austin TX (November 10-13) Tuesday November 12 2013 600 PM-1000 PM

2012 Shrestha R B and R R Pandey 2012 Jointly organized and moderated Member

symposium on ldquoGlobal Collaboration for the Development of Next Generation IPM

Programrdquo for the 60th

annual meeting of ESA Knoxville TN (November 11-14)

Wednesday November 14 2012 800 AM-1200 PM

2010 Shrestha R B and M N Parajulee 2010 Jointly organized and moderated Member

symposium on ldquoMolecular and Other Novel Tools in Ecological Researchrdquo for the 58th

annual meeting of ESA San Diego CA (December 12-15) Wednesday December 15

2010 115 PM-330 PM

Active Memberships in Professional and Honor Societies

Member of Entomological Society of America

Member of Ecological Society of America

Member of Society of Southwestern Entomologists

Member of High Plains Association of Crop Consultants

President of Society of Overseas Nepalese Entomologists

Member of the National Scholars Honor Society

Member of Golden Key International Honor Society TTU Chapter

Member of Honor Society of Agriculture Gamma Sigma Delta TTU Chapter

RB Shrestha Page 9

January 26 2014

9

Member of American Association for Advancement in Science

Founder Member of Nepalese Student Association Texas Tech University

Professional Development Trainings and Workshops

In addition to my formal academic courses I have learned various important skills through

thirteen professional development trainings and workshops The training includes grant

writing training computer programing (R and Python) Integrated Pest Management (IPM)

training Training of Trainers (TOT) and plant biotechnology training Immuno-marking and

Enzyme Linked Immuno Sorbent (ELISA) training microsatellite DNA marker discovery

training teaching enhancement (Blackboard and WebCT) training laboratory safety (handling

radioactive materials pesticide application and shipment of hazardous materials) Compliance

training for conducting regulated Monsanto cotton field trial and training on cotton growth and

development monitoring using COTMAN software

I have also participated in short training courses in professional development such as time

management supervision project management problem solving conflict management scientific

methods information security awareness work place ethics creating discrimination free work

spaces and reporting fraud waste and abuse

Computational or Quantitative Skills and Experience

Through formal course work short training work experience and self-learning exercises I have

acquired computer skills on the application of 29 different computer software and programs

and used them in various research projects My computational skills include statistical data

analysis using SAS SPSS MATLAB SigmaPlot and ArcGIS online course preparation and

teaching using Blackboard and WebCT integrated reference management using EndNote

Medley and Reference Manager documentation database management and technical

communication using Microsoft Office Word Excel Access PowerPoint and Outlook

population genetics and phylogenetic data analysis using PAUP PHYLIP ARLEQUIN

STRUCTURE GenAlEx NTSYS PopGen MEGA AFLP SURV and TotalLab publishing and

graphic design using Adobe Photoshop Illustrator and Paint

RB Shrestha Page 10

January 26 2014

10

Publications and Presentations

1 Peer Reviewed Journal Articles

(15 totals 150 times citied) web httpscholargooglecomcitationsuser=JphkwaMAAAAJamphl=en

11 Shrestha R B and M N Parajulee 2013 Potential cotton aphid Aphis gossypii

population suppression by arthropod predators in upland cotton Journal of Insect Science

20(6)778-788

12 Parajulee M N R B Shrestha J E Slosser and D G Bordovsky 2011 Effects of

skip-row planting pattern and planting date on dryland cotton insect pest abundance and

selected plant parameters Southwestern Entomologist 36 21-39

13 Shrestha R B and M N Parajulee 2010 Effect of tillage and planting date on seasonal

abundance and diversity of predacious ground beetles in cotton Journal of Insect Science

10 (174) 1-16

14 Matis T I M N Parajulee J H Matis and R B Shrestha 2008 A mechanistic model

based analysis of cotton aphid population dynamics data Journal of Agriculture and

Forestry Ecology 10 355-362

15 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Egyptian Journal of Agricultural Research 86 57-81

16 Shrestha R B M N Parajulee O P Perera B E Scheffler and L D Densmore

2007 Characterization of microsatellite loci in the western tarnished plant bug Lygus

hesperus (Knight) (Hemiptera Miridae) Molecular Ecology Notes 7 1342-1344

17 Parajulee M N R B Shrestha and J F Leser 2006 Influence of tillage planting date

and Bt cultivar on seasonal abundance and within-plant distribution patterns of thrips and

cotton fleahoppers in cotton International Journal of Pest Management 52 249-260

18 Parajulee M N R B Shrestha and J F Leser 2006 Dispersion patterns and fixed

precision sequential sampling plans for western flower thrips (Thysanoptera Thripidae)

and cotton fleahoppers (Hemiptera Miridae) in cotton Journal of Economic Entomology

99 568-577

19 Parajulee M N R B Shrestha J F Leser D B Wester and C Blanco 2006

Evaluation of the functional response of selected cotton arthropod predators on bollworm

eggs in the laboratory and effect of temperature on their predation efficiency

Environmental Entomology 35 379-386

110 Chen F G Wu F Ge M N Parajulee and R B Shrestha 2005 Effects of elevated

CO2 and transgenic Bt cotton on plant chemistry performance and feeding of an insect

herbivore the cotton bollworm Entomologia Experimentalis et Applicata 115 341-350

RB Shrestha Page 11

January 26 2014

11

111 Dahal G R B Shrestha and R B Thapa 1997 Species composition and relative

abundance of rice green leafhopper (Nephotettix spp) in different altitude regions of

Nepal International Journal of Pest Management 43(1) 49-58

112 Dahal G A Druka T M Burns L C Villegas Z Fan R B Shrestha and R Hull

1996 Some biological and genomic properties of rice tungro bacilliform badna virus and

rice tungro spherical waika virus from Nepal Annals of Applied Biology 129 (2) 267-

287

113 Pokharel R R G Dahal and R B Shrestha 1994 Association of nematode

populations and virus-like symptoms in papaya Journal of Institute of Agriculture and

Animal Science 15 107-113

114 Dahal G R B Shrestha N K Khatri Z Fan and R Hull 1993 Incidence of virus

diseases of rice in Nepal Journal of Institute of Agriculture and Animal science 14 115-

116

115 Dahal G R R Pokharel and R B Shrestha 1992 A bibliographic database of plant

pathology in Nepal Journal of Institute of Agriculture and Animal Science 13 53-64

2 Papers Published in Refereed Proceedings (2 Total)

21 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) Proceedings World Cotton Research

Conference (CD)

22 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review pp 154 -167

Proceeding of second National Conference of Science and Technology

3 Papers in preparation for submission in Journals (5 total)

31 Shrestha R B S R K Jakka and A J Gassmann 2014 Survivorship of Western Corn

Rootworm problem field population on Bt corn hybrids revealed cross resistance between

Cry3Bb and mCry3A Journal of Economic Entomology (In preparation)

32 Shrestha R B S R K Jakka and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing eCry3A mCry3A

and eCrymCry3A stacked genes Journal of Economic Entomology (In preparation)

33 Jakka S R K R B Shrestha and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing Cry3Bb and

Cry3435Ab Journal of Economic Entomology (In preparation)

RB Shrestha Page 12

January 26 2014

12

34 Shrestha R B M N Parajulee S C Carroll J K Dever M D Arnold and J R

Hagler 2014 Characterization of the intercrop movement behavior of Lygus hesperus

between cotton and alfalfa Environmental Entomology (In preparation)

35 Shrestha R B M N Parajulee L Densmore and M San Francisco 2014

Microsatellite marker based genetic diversity of Lygus hesperus from Texas High Plains

Journal of Insect Science (In preparation)

4 Papers Published in Non-Refereed Proceedings (35 Total)

41 R B Shrestha M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

42 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

43 Parajulee M N R B Shrestha and S C Carroll 2013 Evaluation of organic pesticides

for western flower thrips management in seedling cotton Effect on plant parameters

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

44 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

45 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

46 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

47 M N Parajulee Shrestha R B W O McSpadden and SC Carroll 2011

Development of metapopulation approach for landscape-level Lygus Hesperus

management in Texas pp 220 In Proceedings of World Cotton Research Conference-5

Nov7-11 2011 Mumbai India

48 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2011 Evaluation

of varietal differences in COTMAN compensation capacity values in high-input cotton

subjected to varying levels of Lygus-induced fruit damage pp 825-829 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

RB Shrestha Page 13

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49 Parajulee M N M B Adhikari D L Kerns R B Shrestha and S C Carroll 2011

Insecticide termination rule for western tarnished plant bug in the Texas High Plains pp

1204-1208 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

410 Parajulee M N D Neupane R B Shrestha Y Yang and L T Wilson 2011 Effect

of cotton fleahopper density on pre-flower fruit loss and plant biomass in cotton pp 1095-

1099 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

411 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on Lygus hesperus abundance in adjacent cotton field A field marking study pp

853-858 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

412 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains pp 1180-88 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

413 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton pp 1155-1162 Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

414 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton pp 1163-1169 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

415 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults versus nymphs pp 849-852 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

416 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa pp 873-881

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

417 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions pp 1159-1162 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

418 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris pp 1118-1129

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

419 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip irrigated cotton pp 1251-

RB Shrestha Page 14

January 26 2014

14

1256 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

420 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus bugs from

Texas pp 1693-1699 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

421 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains pp 1700-1703

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

422 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus pp 1443-1446 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

423 Albeldantildeo W A J E Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity pp 1495-1497 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

424 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton pp 1767-1773 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

425 Parajulee M N R B Shrestha S C Carroll P L Bommireddy A M Cranmer and

K F Bronson 2005 Variable rate of irrigation water and nitrogen Potential of site-

specific management of cotton aphids pp 1735-1742 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

426 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton pp 1779-1783 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

427 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

cotton arthropod predators to bollworm eggs in the laboratory pp 1652-1659

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

428 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii p 387

Proceedings 15th

International Plant Protection Congress Foreign Language Press

Beijing China

429 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research pp 1918-1922 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

430 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Seasonal abundance patterns of thrips and fleahoppers in Texas High

RB Shrestha Page 15

January 26 2014

15

Plains cotton pp 1375-1380 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

431 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains pp 970-973 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

432 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetle in the laboratory pp 1060-1063 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

433 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in Texas High Plains pp 1370-1371 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

434 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed pp 1537-1539 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

435 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton pp 1372-1374 Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

5 Published Scientific Reports (23 Total)

51 Parajulee M N S C Carroll R B Shrestha J P Bordovsky 2012 Effect of nitrogen

fertilizer on cotton host-plant quality and its impact on arthropod activity Helms TX

2012

52 Parajulee M N R B Shrestha S Carroll and Wayne Keeling 2012 Cotton yield

response to late-season Lygus plant bug infestations as influenced by cultivar x irrigation

level treatments Lamesa TX 2012

53 Parajulee M N R B Shrestha and S C Carroll 2012 Characterization of Cotton Crop

Response to Thrips Injury for Improved Thrips Management in Texas High Plains Cotton

2012 Annual Report Cotton Incorporated Core Program Project Number 12-364

54 Parajulee M N R B Shrestha and S Carroll 2012 Development of Economic

Threshold and Management Recommendations for Lygus in Texas High Plains Cotton

2012 Annual Report (Year 1) Cotton IncorporatedTexas State Support Program Project

Number 12-112TX

55 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluating varietal

difference in COTMAN compensation capacity value in drip irrigated cotton using Lygus-

RB Shrestha Page 16

January 26 2014

16

induced fruit damage 2011 Final report Cotton Incorporated Core Program Project

Number 09-596 pp 1-16

56 Parajulee M N S C Carroll R BShrestha AM Cranmer and J P Bordovsky 2011

Influence of soil nitrogen level on seasonal activity of cotton arthropods and lint yield

under drip irrigation (field 6h) 2011 Helms Research Farm Annual Report p19

57 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluation of

westerntarnished plant bug as a late season pest of cotton 2011 Final report Cotton

IncorporatedState Support Program Project Number 08-305TX pp 1-18

58 Parajulee M N O McSpadden R B Shrestha S Carroll and W Keeling 2011

Cotton fruitingyield compensation after Lygus induced square loss as influenced by

variety x water treatments Lamesa TX 2011 Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 30-31

59 Parajulee M N B Kesey O McSpadden S Carroll R B Shrestha and W Keeling

2010 Effect of cover crop on arthropod population dynamics in subsurface drip irrigated

cotton at AG-CARES Lamesa TX 2010 Agricultural Complex for Advanced Research

and Extension Systems Annual Report pp45-46

510 Parajulee M N O McSpadden D Neupane R B Shrestha S Carroll and W

Keeling 2010 Cotton fruitingyield compensation after Lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2010 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 47-48

511 Parajulee M N R B Shrestha O McSpadden A Bastola D Neupane S Carroll and

W Keeling 2009 Cotton fruitingyield compensation after lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2009 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 46-47

512 Parajulee M N S C Carroll R BShrestha R J Kesey D M Nesmith and J P

Bordovsky 2009 Influence of soil nitrogen level on seasonal activity of cotton arthropods

and lint yield under drip irrigation 2009 Helms Research Farm Annual Report p18

513 Parajulee M N A Bastola R B Shrestha and W Keeling 2009 Evaluation of cotton

boll susceptibility to Lygus bugs as influenced by cultivar x irrigation interaction

AGCARES Lamesa TX 2009Agricultural Complex for Advanced Research and

Extension Systems Annual Report pp 48-49

514 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2004 Influence of soil nitrogen level on seasonal activity of cotton arthropods in drip

irrigation system Helms Research Farm Annual Report p 25

515 Parajulee M N J F Leser R B Shrestha A K Barman and R K Boman 2004

Evaluating the merits of sub-threshold effects of the Bollgard technology on the

economics of bollworm management Helms Research Farm Annual Report p 14

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 7: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 7

January 26 2014

7

Postharvest loss management program such as grain storage improvement zero-energy

potato storage and cellar fruit storage

Training of junior agriculture technicians and progressive lead farmers

Distribution of posters leaflets booklets pamphlets and calendars

Crop protection training kits and subsidies distribution

Collaboration with research scientists in National Agriculture Research Council for

technology update and feedback on new crop protection technologies

Annual progress report publication (1995-2001)

Growers competition and award program

Collaborative Team Work Experiences

I have worked in close collaboration with several scientists and people from other national and

international laboratories and have published scientific papers coauthored with more than 30

people from different institutions

Leadership and Service to Professional Societies

Contributions to Professional Societies

2013 ESA Annual Meeting Member Symposium organizer Austin TX (November 10-13)

2013 ESA Annual Meeting Graduate Student Ten-Minute Paper competition session

moderator Austin TX (November 10-13) P-IE Section

Monday November 11 2013 800 AM-1200 PM

2012-2013 President Society of Overseas Nepalese Entomologists (SONE)

2012 ESA Annual Meeting Member Symposium organizer Knoxville TN (Nov 11-14)

2012 ESA Annual Meeting Symposium moderator Knoxville TN (November 11-14) P-IE

Section Environmental Entomology Symposium Tuesday November 13 2012 800

AM-1215 PM

2011-2012 Vice president Society of Overseas Nepalese Entomologists (SONE)

2011 ESA Annual Meeting Student Oral Competition Judge Reno NV (November 13-16)

2010 ESA Annual Meeting Symposium Organizer and Moderator San Diego CA

(December 12-16)

2010 ESA Annual Meeting Student Volunteer San Diego CA (December 12-16)

2009 ESA-SWB Annual Meeting Student Volunteer Stillwater OK (February 23-26)

2009 ESA-SWB Student Affairs Committee Member

2009 ESA Annual Meeting Student Volunteer Indianapolis IN (December 12-16)

RB Shrestha Page 8

January 26 2014

8

2009 ESA Annual Meeting Student Poster Competition Judge Indianapolis IN (December

12-16)

2008 ESA Annual Meeting Student Volunteer Reno NV (December 12-16)

2006 ESA-SWB Annual Meeting Student Volunteer Austin TX (February 28-March 2)

2006 ESA-SWB Annual Meeting Student Poster Competition Judge Austin TX (February

28-March 2)

2004 ESA-SWB Annual Meeting Student Volunteer Lubbock TX (February 23-26)

2004 ESA-SWB Annual Meeting Student Affairs Committee Member Lubbock TX

(February 23-26)

Conference Symposium Management

2014 Shrestha R B S R K Jakka and A J Gassmann 2014 co-organizing a symposium

(proposal accepted) in ESA NCB Annual Meeting Proposed title Advancements and

Challenges in Western Corn Rootworm Management Des Moines IA (March 9-10

2014)

2013 Shrestha R B B K Gautam J Rijal and S Gautam 2013 Jointly organized and

moderated Member symposium on ldquoPromoting Entomological Collaboration through

Inter-societal Network and Information Sharingrdquo for the 61th

annual meeting of ESA

Austin TX (November 10-13) Tuesday November 12 2013 600 PM-1000 PM

2012 Shrestha R B and R R Pandey 2012 Jointly organized and moderated Member

symposium on ldquoGlobal Collaboration for the Development of Next Generation IPM

Programrdquo for the 60th

annual meeting of ESA Knoxville TN (November 11-14)

Wednesday November 14 2012 800 AM-1200 PM

2010 Shrestha R B and M N Parajulee 2010 Jointly organized and moderated Member

symposium on ldquoMolecular and Other Novel Tools in Ecological Researchrdquo for the 58th

annual meeting of ESA San Diego CA (December 12-15) Wednesday December 15

2010 115 PM-330 PM

Active Memberships in Professional and Honor Societies

Member of Entomological Society of America

Member of Ecological Society of America

Member of Society of Southwestern Entomologists

Member of High Plains Association of Crop Consultants

President of Society of Overseas Nepalese Entomologists

Member of the National Scholars Honor Society

Member of Golden Key International Honor Society TTU Chapter

Member of Honor Society of Agriculture Gamma Sigma Delta TTU Chapter

RB Shrestha Page 9

January 26 2014

9

Member of American Association for Advancement in Science

Founder Member of Nepalese Student Association Texas Tech University

Professional Development Trainings and Workshops

In addition to my formal academic courses I have learned various important skills through

thirteen professional development trainings and workshops The training includes grant

writing training computer programing (R and Python) Integrated Pest Management (IPM)

training Training of Trainers (TOT) and plant biotechnology training Immuno-marking and

Enzyme Linked Immuno Sorbent (ELISA) training microsatellite DNA marker discovery

training teaching enhancement (Blackboard and WebCT) training laboratory safety (handling

radioactive materials pesticide application and shipment of hazardous materials) Compliance

training for conducting regulated Monsanto cotton field trial and training on cotton growth and

development monitoring using COTMAN software

I have also participated in short training courses in professional development such as time

management supervision project management problem solving conflict management scientific

methods information security awareness work place ethics creating discrimination free work

spaces and reporting fraud waste and abuse

Computational or Quantitative Skills and Experience

Through formal course work short training work experience and self-learning exercises I have

acquired computer skills on the application of 29 different computer software and programs

and used them in various research projects My computational skills include statistical data

analysis using SAS SPSS MATLAB SigmaPlot and ArcGIS online course preparation and

teaching using Blackboard and WebCT integrated reference management using EndNote

Medley and Reference Manager documentation database management and technical

communication using Microsoft Office Word Excel Access PowerPoint and Outlook

population genetics and phylogenetic data analysis using PAUP PHYLIP ARLEQUIN

STRUCTURE GenAlEx NTSYS PopGen MEGA AFLP SURV and TotalLab publishing and

graphic design using Adobe Photoshop Illustrator and Paint

RB Shrestha Page 10

January 26 2014

10

Publications and Presentations

1 Peer Reviewed Journal Articles

(15 totals 150 times citied) web httpscholargooglecomcitationsuser=JphkwaMAAAAJamphl=en

11 Shrestha R B and M N Parajulee 2013 Potential cotton aphid Aphis gossypii

population suppression by arthropod predators in upland cotton Journal of Insect Science

20(6)778-788

12 Parajulee M N R B Shrestha J E Slosser and D G Bordovsky 2011 Effects of

skip-row planting pattern and planting date on dryland cotton insect pest abundance and

selected plant parameters Southwestern Entomologist 36 21-39

13 Shrestha R B and M N Parajulee 2010 Effect of tillage and planting date on seasonal

abundance and diversity of predacious ground beetles in cotton Journal of Insect Science

10 (174) 1-16

14 Matis T I M N Parajulee J H Matis and R B Shrestha 2008 A mechanistic model

based analysis of cotton aphid population dynamics data Journal of Agriculture and

Forestry Ecology 10 355-362

15 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Egyptian Journal of Agricultural Research 86 57-81

16 Shrestha R B M N Parajulee O P Perera B E Scheffler and L D Densmore

2007 Characterization of microsatellite loci in the western tarnished plant bug Lygus

hesperus (Knight) (Hemiptera Miridae) Molecular Ecology Notes 7 1342-1344

17 Parajulee M N R B Shrestha and J F Leser 2006 Influence of tillage planting date

and Bt cultivar on seasonal abundance and within-plant distribution patterns of thrips and

cotton fleahoppers in cotton International Journal of Pest Management 52 249-260

18 Parajulee M N R B Shrestha and J F Leser 2006 Dispersion patterns and fixed

precision sequential sampling plans for western flower thrips (Thysanoptera Thripidae)

and cotton fleahoppers (Hemiptera Miridae) in cotton Journal of Economic Entomology

99 568-577

19 Parajulee M N R B Shrestha J F Leser D B Wester and C Blanco 2006

Evaluation of the functional response of selected cotton arthropod predators on bollworm

eggs in the laboratory and effect of temperature on their predation efficiency

Environmental Entomology 35 379-386

110 Chen F G Wu F Ge M N Parajulee and R B Shrestha 2005 Effects of elevated

CO2 and transgenic Bt cotton on plant chemistry performance and feeding of an insect

herbivore the cotton bollworm Entomologia Experimentalis et Applicata 115 341-350

RB Shrestha Page 11

January 26 2014

11

111 Dahal G R B Shrestha and R B Thapa 1997 Species composition and relative

abundance of rice green leafhopper (Nephotettix spp) in different altitude regions of

Nepal International Journal of Pest Management 43(1) 49-58

112 Dahal G A Druka T M Burns L C Villegas Z Fan R B Shrestha and R Hull

1996 Some biological and genomic properties of rice tungro bacilliform badna virus and

rice tungro spherical waika virus from Nepal Annals of Applied Biology 129 (2) 267-

287

113 Pokharel R R G Dahal and R B Shrestha 1994 Association of nematode

populations and virus-like symptoms in papaya Journal of Institute of Agriculture and

Animal Science 15 107-113

114 Dahal G R B Shrestha N K Khatri Z Fan and R Hull 1993 Incidence of virus

diseases of rice in Nepal Journal of Institute of Agriculture and Animal science 14 115-

116

115 Dahal G R R Pokharel and R B Shrestha 1992 A bibliographic database of plant

pathology in Nepal Journal of Institute of Agriculture and Animal Science 13 53-64

2 Papers Published in Refereed Proceedings (2 Total)

21 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) Proceedings World Cotton Research

Conference (CD)

22 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review pp 154 -167

Proceeding of second National Conference of Science and Technology

3 Papers in preparation for submission in Journals (5 total)

31 Shrestha R B S R K Jakka and A J Gassmann 2014 Survivorship of Western Corn

Rootworm problem field population on Bt corn hybrids revealed cross resistance between

Cry3Bb and mCry3A Journal of Economic Entomology (In preparation)

32 Shrestha R B S R K Jakka and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing eCry3A mCry3A

and eCrymCry3A stacked genes Journal of Economic Entomology (In preparation)

33 Jakka S R K R B Shrestha and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing Cry3Bb and

Cry3435Ab Journal of Economic Entomology (In preparation)

RB Shrestha Page 12

January 26 2014

12

34 Shrestha R B M N Parajulee S C Carroll J K Dever M D Arnold and J R

Hagler 2014 Characterization of the intercrop movement behavior of Lygus hesperus

between cotton and alfalfa Environmental Entomology (In preparation)

35 Shrestha R B M N Parajulee L Densmore and M San Francisco 2014

Microsatellite marker based genetic diversity of Lygus hesperus from Texas High Plains

Journal of Insect Science (In preparation)

4 Papers Published in Non-Refereed Proceedings (35 Total)

41 R B Shrestha M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

42 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

43 Parajulee M N R B Shrestha and S C Carroll 2013 Evaluation of organic pesticides

for western flower thrips management in seedling cotton Effect on plant parameters

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

44 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

45 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

46 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

47 M N Parajulee Shrestha R B W O McSpadden and SC Carroll 2011

Development of metapopulation approach for landscape-level Lygus Hesperus

management in Texas pp 220 In Proceedings of World Cotton Research Conference-5

Nov7-11 2011 Mumbai India

48 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2011 Evaluation

of varietal differences in COTMAN compensation capacity values in high-input cotton

subjected to varying levels of Lygus-induced fruit damage pp 825-829 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

RB Shrestha Page 13

January 26 2014

13

49 Parajulee M N M B Adhikari D L Kerns R B Shrestha and S C Carroll 2011

Insecticide termination rule for western tarnished plant bug in the Texas High Plains pp

1204-1208 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

410 Parajulee M N D Neupane R B Shrestha Y Yang and L T Wilson 2011 Effect

of cotton fleahopper density on pre-flower fruit loss and plant biomass in cotton pp 1095-

1099 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

411 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on Lygus hesperus abundance in adjacent cotton field A field marking study pp

853-858 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

412 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains pp 1180-88 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

413 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton pp 1155-1162 Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

414 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton pp 1163-1169 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

415 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults versus nymphs pp 849-852 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

416 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa pp 873-881

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

417 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions pp 1159-1162 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

418 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris pp 1118-1129

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

419 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip irrigated cotton pp 1251-

RB Shrestha Page 14

January 26 2014

14

1256 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

420 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus bugs from

Texas pp 1693-1699 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

421 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains pp 1700-1703

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

422 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus pp 1443-1446 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

423 Albeldantildeo W A J E Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity pp 1495-1497 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

424 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton pp 1767-1773 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

425 Parajulee M N R B Shrestha S C Carroll P L Bommireddy A M Cranmer and

K F Bronson 2005 Variable rate of irrigation water and nitrogen Potential of site-

specific management of cotton aphids pp 1735-1742 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

426 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton pp 1779-1783 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

427 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

cotton arthropod predators to bollworm eggs in the laboratory pp 1652-1659

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

428 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii p 387

Proceedings 15th

International Plant Protection Congress Foreign Language Press

Beijing China

429 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research pp 1918-1922 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

430 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Seasonal abundance patterns of thrips and fleahoppers in Texas High

RB Shrestha Page 15

January 26 2014

15

Plains cotton pp 1375-1380 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

431 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains pp 970-973 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

432 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetle in the laboratory pp 1060-1063 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

433 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in Texas High Plains pp 1370-1371 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

434 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed pp 1537-1539 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

435 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton pp 1372-1374 Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

5 Published Scientific Reports (23 Total)

51 Parajulee M N S C Carroll R B Shrestha J P Bordovsky 2012 Effect of nitrogen

fertilizer on cotton host-plant quality and its impact on arthropod activity Helms TX

2012

52 Parajulee M N R B Shrestha S Carroll and Wayne Keeling 2012 Cotton yield

response to late-season Lygus plant bug infestations as influenced by cultivar x irrigation

level treatments Lamesa TX 2012

53 Parajulee M N R B Shrestha and S C Carroll 2012 Characterization of Cotton Crop

Response to Thrips Injury for Improved Thrips Management in Texas High Plains Cotton

2012 Annual Report Cotton Incorporated Core Program Project Number 12-364

54 Parajulee M N R B Shrestha and S Carroll 2012 Development of Economic

Threshold and Management Recommendations for Lygus in Texas High Plains Cotton

2012 Annual Report (Year 1) Cotton IncorporatedTexas State Support Program Project

Number 12-112TX

55 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluating varietal

difference in COTMAN compensation capacity value in drip irrigated cotton using Lygus-

RB Shrestha Page 16

January 26 2014

16

induced fruit damage 2011 Final report Cotton Incorporated Core Program Project

Number 09-596 pp 1-16

56 Parajulee M N S C Carroll R BShrestha AM Cranmer and J P Bordovsky 2011

Influence of soil nitrogen level on seasonal activity of cotton arthropods and lint yield

under drip irrigation (field 6h) 2011 Helms Research Farm Annual Report p19

57 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluation of

westerntarnished plant bug as a late season pest of cotton 2011 Final report Cotton

IncorporatedState Support Program Project Number 08-305TX pp 1-18

58 Parajulee M N O McSpadden R B Shrestha S Carroll and W Keeling 2011

Cotton fruitingyield compensation after Lygus induced square loss as influenced by

variety x water treatments Lamesa TX 2011 Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 30-31

59 Parajulee M N B Kesey O McSpadden S Carroll R B Shrestha and W Keeling

2010 Effect of cover crop on arthropod population dynamics in subsurface drip irrigated

cotton at AG-CARES Lamesa TX 2010 Agricultural Complex for Advanced Research

and Extension Systems Annual Report pp45-46

510 Parajulee M N O McSpadden D Neupane R B Shrestha S Carroll and W

Keeling 2010 Cotton fruitingyield compensation after Lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2010 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 47-48

511 Parajulee M N R B Shrestha O McSpadden A Bastola D Neupane S Carroll and

W Keeling 2009 Cotton fruitingyield compensation after lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2009 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 46-47

512 Parajulee M N S C Carroll R BShrestha R J Kesey D M Nesmith and J P

Bordovsky 2009 Influence of soil nitrogen level on seasonal activity of cotton arthropods

and lint yield under drip irrigation 2009 Helms Research Farm Annual Report p18

513 Parajulee M N A Bastola R B Shrestha and W Keeling 2009 Evaluation of cotton

boll susceptibility to Lygus bugs as influenced by cultivar x irrigation interaction

AGCARES Lamesa TX 2009Agricultural Complex for Advanced Research and

Extension Systems Annual Report pp 48-49

514 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2004 Influence of soil nitrogen level on seasonal activity of cotton arthropods in drip

irrigation system Helms Research Farm Annual Report p 25

515 Parajulee M N J F Leser R B Shrestha A K Barman and R K Boman 2004

Evaluating the merits of sub-threshold effects of the Bollgard technology on the

economics of bollworm management Helms Research Farm Annual Report p 14

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 8: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 8

January 26 2014

8

2009 ESA Annual Meeting Student Poster Competition Judge Indianapolis IN (December

12-16)

2008 ESA Annual Meeting Student Volunteer Reno NV (December 12-16)

2006 ESA-SWB Annual Meeting Student Volunteer Austin TX (February 28-March 2)

2006 ESA-SWB Annual Meeting Student Poster Competition Judge Austin TX (February

28-March 2)

2004 ESA-SWB Annual Meeting Student Volunteer Lubbock TX (February 23-26)

2004 ESA-SWB Annual Meeting Student Affairs Committee Member Lubbock TX

(February 23-26)

Conference Symposium Management

2014 Shrestha R B S R K Jakka and A J Gassmann 2014 co-organizing a symposium

(proposal accepted) in ESA NCB Annual Meeting Proposed title Advancements and

Challenges in Western Corn Rootworm Management Des Moines IA (March 9-10

2014)

2013 Shrestha R B B K Gautam J Rijal and S Gautam 2013 Jointly organized and

moderated Member symposium on ldquoPromoting Entomological Collaboration through

Inter-societal Network and Information Sharingrdquo for the 61th

annual meeting of ESA

Austin TX (November 10-13) Tuesday November 12 2013 600 PM-1000 PM

2012 Shrestha R B and R R Pandey 2012 Jointly organized and moderated Member

symposium on ldquoGlobal Collaboration for the Development of Next Generation IPM

Programrdquo for the 60th

annual meeting of ESA Knoxville TN (November 11-14)

Wednesday November 14 2012 800 AM-1200 PM

2010 Shrestha R B and M N Parajulee 2010 Jointly organized and moderated Member

symposium on ldquoMolecular and Other Novel Tools in Ecological Researchrdquo for the 58th

annual meeting of ESA San Diego CA (December 12-15) Wednesday December 15

2010 115 PM-330 PM

Active Memberships in Professional and Honor Societies

Member of Entomological Society of America

Member of Ecological Society of America

Member of Society of Southwestern Entomologists

Member of High Plains Association of Crop Consultants

President of Society of Overseas Nepalese Entomologists

Member of the National Scholars Honor Society

Member of Golden Key International Honor Society TTU Chapter

Member of Honor Society of Agriculture Gamma Sigma Delta TTU Chapter

RB Shrestha Page 9

January 26 2014

9

Member of American Association for Advancement in Science

Founder Member of Nepalese Student Association Texas Tech University

Professional Development Trainings and Workshops

In addition to my formal academic courses I have learned various important skills through

thirteen professional development trainings and workshops The training includes grant

writing training computer programing (R and Python) Integrated Pest Management (IPM)

training Training of Trainers (TOT) and plant biotechnology training Immuno-marking and

Enzyme Linked Immuno Sorbent (ELISA) training microsatellite DNA marker discovery

training teaching enhancement (Blackboard and WebCT) training laboratory safety (handling

radioactive materials pesticide application and shipment of hazardous materials) Compliance

training for conducting regulated Monsanto cotton field trial and training on cotton growth and

development monitoring using COTMAN software

I have also participated in short training courses in professional development such as time

management supervision project management problem solving conflict management scientific

methods information security awareness work place ethics creating discrimination free work

spaces and reporting fraud waste and abuse

Computational or Quantitative Skills and Experience

Through formal course work short training work experience and self-learning exercises I have

acquired computer skills on the application of 29 different computer software and programs

and used them in various research projects My computational skills include statistical data

analysis using SAS SPSS MATLAB SigmaPlot and ArcGIS online course preparation and

teaching using Blackboard and WebCT integrated reference management using EndNote

Medley and Reference Manager documentation database management and technical

communication using Microsoft Office Word Excel Access PowerPoint and Outlook

population genetics and phylogenetic data analysis using PAUP PHYLIP ARLEQUIN

STRUCTURE GenAlEx NTSYS PopGen MEGA AFLP SURV and TotalLab publishing and

graphic design using Adobe Photoshop Illustrator and Paint

RB Shrestha Page 10

January 26 2014

10

Publications and Presentations

1 Peer Reviewed Journal Articles

(15 totals 150 times citied) web httpscholargooglecomcitationsuser=JphkwaMAAAAJamphl=en

11 Shrestha R B and M N Parajulee 2013 Potential cotton aphid Aphis gossypii

population suppression by arthropod predators in upland cotton Journal of Insect Science

20(6)778-788

12 Parajulee M N R B Shrestha J E Slosser and D G Bordovsky 2011 Effects of

skip-row planting pattern and planting date on dryland cotton insect pest abundance and

selected plant parameters Southwestern Entomologist 36 21-39

13 Shrestha R B and M N Parajulee 2010 Effect of tillage and planting date on seasonal

abundance and diversity of predacious ground beetles in cotton Journal of Insect Science

10 (174) 1-16

14 Matis T I M N Parajulee J H Matis and R B Shrestha 2008 A mechanistic model

based analysis of cotton aphid population dynamics data Journal of Agriculture and

Forestry Ecology 10 355-362

15 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Egyptian Journal of Agricultural Research 86 57-81

16 Shrestha R B M N Parajulee O P Perera B E Scheffler and L D Densmore

2007 Characterization of microsatellite loci in the western tarnished plant bug Lygus

hesperus (Knight) (Hemiptera Miridae) Molecular Ecology Notes 7 1342-1344

17 Parajulee M N R B Shrestha and J F Leser 2006 Influence of tillage planting date

and Bt cultivar on seasonal abundance and within-plant distribution patterns of thrips and

cotton fleahoppers in cotton International Journal of Pest Management 52 249-260

18 Parajulee M N R B Shrestha and J F Leser 2006 Dispersion patterns and fixed

precision sequential sampling plans for western flower thrips (Thysanoptera Thripidae)

and cotton fleahoppers (Hemiptera Miridae) in cotton Journal of Economic Entomology

99 568-577

19 Parajulee M N R B Shrestha J F Leser D B Wester and C Blanco 2006

Evaluation of the functional response of selected cotton arthropod predators on bollworm

eggs in the laboratory and effect of temperature on their predation efficiency

Environmental Entomology 35 379-386

110 Chen F G Wu F Ge M N Parajulee and R B Shrestha 2005 Effects of elevated

CO2 and transgenic Bt cotton on plant chemistry performance and feeding of an insect

herbivore the cotton bollworm Entomologia Experimentalis et Applicata 115 341-350

RB Shrestha Page 11

January 26 2014

11

111 Dahal G R B Shrestha and R B Thapa 1997 Species composition and relative

abundance of rice green leafhopper (Nephotettix spp) in different altitude regions of

Nepal International Journal of Pest Management 43(1) 49-58

112 Dahal G A Druka T M Burns L C Villegas Z Fan R B Shrestha and R Hull

1996 Some biological and genomic properties of rice tungro bacilliform badna virus and

rice tungro spherical waika virus from Nepal Annals of Applied Biology 129 (2) 267-

287

113 Pokharel R R G Dahal and R B Shrestha 1994 Association of nematode

populations and virus-like symptoms in papaya Journal of Institute of Agriculture and

Animal Science 15 107-113

114 Dahal G R B Shrestha N K Khatri Z Fan and R Hull 1993 Incidence of virus

diseases of rice in Nepal Journal of Institute of Agriculture and Animal science 14 115-

116

115 Dahal G R R Pokharel and R B Shrestha 1992 A bibliographic database of plant

pathology in Nepal Journal of Institute of Agriculture and Animal Science 13 53-64

2 Papers Published in Refereed Proceedings (2 Total)

21 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) Proceedings World Cotton Research

Conference (CD)

22 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review pp 154 -167

Proceeding of second National Conference of Science and Technology

3 Papers in preparation for submission in Journals (5 total)

31 Shrestha R B S R K Jakka and A J Gassmann 2014 Survivorship of Western Corn

Rootworm problem field population on Bt corn hybrids revealed cross resistance between

Cry3Bb and mCry3A Journal of Economic Entomology (In preparation)

32 Shrestha R B S R K Jakka and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing eCry3A mCry3A

and eCrymCry3A stacked genes Journal of Economic Entomology (In preparation)

33 Jakka S R K R B Shrestha and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing Cry3Bb and

Cry3435Ab Journal of Economic Entomology (In preparation)

RB Shrestha Page 12

January 26 2014

12

34 Shrestha R B M N Parajulee S C Carroll J K Dever M D Arnold and J R

Hagler 2014 Characterization of the intercrop movement behavior of Lygus hesperus

between cotton and alfalfa Environmental Entomology (In preparation)

35 Shrestha R B M N Parajulee L Densmore and M San Francisco 2014

Microsatellite marker based genetic diversity of Lygus hesperus from Texas High Plains

Journal of Insect Science (In preparation)

4 Papers Published in Non-Refereed Proceedings (35 Total)

41 R B Shrestha M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

42 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

43 Parajulee M N R B Shrestha and S C Carroll 2013 Evaluation of organic pesticides

for western flower thrips management in seedling cotton Effect on plant parameters

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

44 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

45 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

46 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

47 M N Parajulee Shrestha R B W O McSpadden and SC Carroll 2011

Development of metapopulation approach for landscape-level Lygus Hesperus

management in Texas pp 220 In Proceedings of World Cotton Research Conference-5

Nov7-11 2011 Mumbai India

48 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2011 Evaluation

of varietal differences in COTMAN compensation capacity values in high-input cotton

subjected to varying levels of Lygus-induced fruit damage pp 825-829 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

RB Shrestha Page 13

January 26 2014

13

49 Parajulee M N M B Adhikari D L Kerns R B Shrestha and S C Carroll 2011

Insecticide termination rule for western tarnished plant bug in the Texas High Plains pp

1204-1208 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

410 Parajulee M N D Neupane R B Shrestha Y Yang and L T Wilson 2011 Effect

of cotton fleahopper density on pre-flower fruit loss and plant biomass in cotton pp 1095-

1099 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

411 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on Lygus hesperus abundance in adjacent cotton field A field marking study pp

853-858 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

412 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains pp 1180-88 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

413 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton pp 1155-1162 Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

414 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton pp 1163-1169 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

415 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults versus nymphs pp 849-852 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

416 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa pp 873-881

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

417 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions pp 1159-1162 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

418 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris pp 1118-1129

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

419 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip irrigated cotton pp 1251-

RB Shrestha Page 14

January 26 2014

14

1256 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

420 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus bugs from

Texas pp 1693-1699 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

421 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains pp 1700-1703

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

422 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus pp 1443-1446 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

423 Albeldantildeo W A J E Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity pp 1495-1497 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

424 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton pp 1767-1773 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

425 Parajulee M N R B Shrestha S C Carroll P L Bommireddy A M Cranmer and

K F Bronson 2005 Variable rate of irrigation water and nitrogen Potential of site-

specific management of cotton aphids pp 1735-1742 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

426 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton pp 1779-1783 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

427 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

cotton arthropod predators to bollworm eggs in the laboratory pp 1652-1659

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

428 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii p 387

Proceedings 15th

International Plant Protection Congress Foreign Language Press

Beijing China

429 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research pp 1918-1922 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

430 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Seasonal abundance patterns of thrips and fleahoppers in Texas High

RB Shrestha Page 15

January 26 2014

15

Plains cotton pp 1375-1380 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

431 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains pp 970-973 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

432 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetle in the laboratory pp 1060-1063 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

433 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in Texas High Plains pp 1370-1371 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

434 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed pp 1537-1539 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

435 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton pp 1372-1374 Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

5 Published Scientific Reports (23 Total)

51 Parajulee M N S C Carroll R B Shrestha J P Bordovsky 2012 Effect of nitrogen

fertilizer on cotton host-plant quality and its impact on arthropod activity Helms TX

2012

52 Parajulee M N R B Shrestha S Carroll and Wayne Keeling 2012 Cotton yield

response to late-season Lygus plant bug infestations as influenced by cultivar x irrigation

level treatments Lamesa TX 2012

53 Parajulee M N R B Shrestha and S C Carroll 2012 Characterization of Cotton Crop

Response to Thrips Injury for Improved Thrips Management in Texas High Plains Cotton

2012 Annual Report Cotton Incorporated Core Program Project Number 12-364

54 Parajulee M N R B Shrestha and S Carroll 2012 Development of Economic

Threshold and Management Recommendations for Lygus in Texas High Plains Cotton

2012 Annual Report (Year 1) Cotton IncorporatedTexas State Support Program Project

Number 12-112TX

55 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluating varietal

difference in COTMAN compensation capacity value in drip irrigated cotton using Lygus-

RB Shrestha Page 16

January 26 2014

16

induced fruit damage 2011 Final report Cotton Incorporated Core Program Project

Number 09-596 pp 1-16

56 Parajulee M N S C Carroll R BShrestha AM Cranmer and J P Bordovsky 2011

Influence of soil nitrogen level on seasonal activity of cotton arthropods and lint yield

under drip irrigation (field 6h) 2011 Helms Research Farm Annual Report p19

57 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluation of

westerntarnished plant bug as a late season pest of cotton 2011 Final report Cotton

IncorporatedState Support Program Project Number 08-305TX pp 1-18

58 Parajulee M N O McSpadden R B Shrestha S Carroll and W Keeling 2011

Cotton fruitingyield compensation after Lygus induced square loss as influenced by

variety x water treatments Lamesa TX 2011 Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 30-31

59 Parajulee M N B Kesey O McSpadden S Carroll R B Shrestha and W Keeling

2010 Effect of cover crop on arthropod population dynamics in subsurface drip irrigated

cotton at AG-CARES Lamesa TX 2010 Agricultural Complex for Advanced Research

and Extension Systems Annual Report pp45-46

510 Parajulee M N O McSpadden D Neupane R B Shrestha S Carroll and W

Keeling 2010 Cotton fruitingyield compensation after Lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2010 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 47-48

511 Parajulee M N R B Shrestha O McSpadden A Bastola D Neupane S Carroll and

W Keeling 2009 Cotton fruitingyield compensation after lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2009 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 46-47

512 Parajulee M N S C Carroll R BShrestha R J Kesey D M Nesmith and J P

Bordovsky 2009 Influence of soil nitrogen level on seasonal activity of cotton arthropods

and lint yield under drip irrigation 2009 Helms Research Farm Annual Report p18

513 Parajulee M N A Bastola R B Shrestha and W Keeling 2009 Evaluation of cotton

boll susceptibility to Lygus bugs as influenced by cultivar x irrigation interaction

AGCARES Lamesa TX 2009Agricultural Complex for Advanced Research and

Extension Systems Annual Report pp 48-49

514 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2004 Influence of soil nitrogen level on seasonal activity of cotton arthropods in drip

irrigation system Helms Research Farm Annual Report p 25

515 Parajulee M N J F Leser R B Shrestha A K Barman and R K Boman 2004

Evaluating the merits of sub-threshold effects of the Bollgard technology on the

economics of bollworm management Helms Research Farm Annual Report p 14

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 9: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 9

January 26 2014

9

Member of American Association for Advancement in Science

Founder Member of Nepalese Student Association Texas Tech University

Professional Development Trainings and Workshops

In addition to my formal academic courses I have learned various important skills through

thirteen professional development trainings and workshops The training includes grant

writing training computer programing (R and Python) Integrated Pest Management (IPM)

training Training of Trainers (TOT) and plant biotechnology training Immuno-marking and

Enzyme Linked Immuno Sorbent (ELISA) training microsatellite DNA marker discovery

training teaching enhancement (Blackboard and WebCT) training laboratory safety (handling

radioactive materials pesticide application and shipment of hazardous materials) Compliance

training for conducting regulated Monsanto cotton field trial and training on cotton growth and

development monitoring using COTMAN software

I have also participated in short training courses in professional development such as time

management supervision project management problem solving conflict management scientific

methods information security awareness work place ethics creating discrimination free work

spaces and reporting fraud waste and abuse

Computational or Quantitative Skills and Experience

Through formal course work short training work experience and self-learning exercises I have

acquired computer skills on the application of 29 different computer software and programs

and used them in various research projects My computational skills include statistical data

analysis using SAS SPSS MATLAB SigmaPlot and ArcGIS online course preparation and

teaching using Blackboard and WebCT integrated reference management using EndNote

Medley and Reference Manager documentation database management and technical

communication using Microsoft Office Word Excel Access PowerPoint and Outlook

population genetics and phylogenetic data analysis using PAUP PHYLIP ARLEQUIN

STRUCTURE GenAlEx NTSYS PopGen MEGA AFLP SURV and TotalLab publishing and

graphic design using Adobe Photoshop Illustrator and Paint

RB Shrestha Page 10

January 26 2014

10

Publications and Presentations

1 Peer Reviewed Journal Articles

(15 totals 150 times citied) web httpscholargooglecomcitationsuser=JphkwaMAAAAJamphl=en

11 Shrestha R B and M N Parajulee 2013 Potential cotton aphid Aphis gossypii

population suppression by arthropod predators in upland cotton Journal of Insect Science

20(6)778-788

12 Parajulee M N R B Shrestha J E Slosser and D G Bordovsky 2011 Effects of

skip-row planting pattern and planting date on dryland cotton insect pest abundance and

selected plant parameters Southwestern Entomologist 36 21-39

13 Shrestha R B and M N Parajulee 2010 Effect of tillage and planting date on seasonal

abundance and diversity of predacious ground beetles in cotton Journal of Insect Science

10 (174) 1-16

14 Matis T I M N Parajulee J H Matis and R B Shrestha 2008 A mechanistic model

based analysis of cotton aphid population dynamics data Journal of Agriculture and

Forestry Ecology 10 355-362

15 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Egyptian Journal of Agricultural Research 86 57-81

16 Shrestha R B M N Parajulee O P Perera B E Scheffler and L D Densmore

2007 Characterization of microsatellite loci in the western tarnished plant bug Lygus

hesperus (Knight) (Hemiptera Miridae) Molecular Ecology Notes 7 1342-1344

17 Parajulee M N R B Shrestha and J F Leser 2006 Influence of tillage planting date

and Bt cultivar on seasonal abundance and within-plant distribution patterns of thrips and

cotton fleahoppers in cotton International Journal of Pest Management 52 249-260

18 Parajulee M N R B Shrestha and J F Leser 2006 Dispersion patterns and fixed

precision sequential sampling plans for western flower thrips (Thysanoptera Thripidae)

and cotton fleahoppers (Hemiptera Miridae) in cotton Journal of Economic Entomology

99 568-577

19 Parajulee M N R B Shrestha J F Leser D B Wester and C Blanco 2006

Evaluation of the functional response of selected cotton arthropod predators on bollworm

eggs in the laboratory and effect of temperature on their predation efficiency

Environmental Entomology 35 379-386

110 Chen F G Wu F Ge M N Parajulee and R B Shrestha 2005 Effects of elevated

CO2 and transgenic Bt cotton on plant chemistry performance and feeding of an insect

herbivore the cotton bollworm Entomologia Experimentalis et Applicata 115 341-350

RB Shrestha Page 11

January 26 2014

11

111 Dahal G R B Shrestha and R B Thapa 1997 Species composition and relative

abundance of rice green leafhopper (Nephotettix spp) in different altitude regions of

Nepal International Journal of Pest Management 43(1) 49-58

112 Dahal G A Druka T M Burns L C Villegas Z Fan R B Shrestha and R Hull

1996 Some biological and genomic properties of rice tungro bacilliform badna virus and

rice tungro spherical waika virus from Nepal Annals of Applied Biology 129 (2) 267-

287

113 Pokharel R R G Dahal and R B Shrestha 1994 Association of nematode

populations and virus-like symptoms in papaya Journal of Institute of Agriculture and

Animal Science 15 107-113

114 Dahal G R B Shrestha N K Khatri Z Fan and R Hull 1993 Incidence of virus

diseases of rice in Nepal Journal of Institute of Agriculture and Animal science 14 115-

116

115 Dahal G R R Pokharel and R B Shrestha 1992 A bibliographic database of plant

pathology in Nepal Journal of Institute of Agriculture and Animal Science 13 53-64

2 Papers Published in Refereed Proceedings (2 Total)

21 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) Proceedings World Cotton Research

Conference (CD)

22 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review pp 154 -167

Proceeding of second National Conference of Science and Technology

3 Papers in preparation for submission in Journals (5 total)

31 Shrestha R B S R K Jakka and A J Gassmann 2014 Survivorship of Western Corn

Rootworm problem field population on Bt corn hybrids revealed cross resistance between

Cry3Bb and mCry3A Journal of Economic Entomology (In preparation)

32 Shrestha R B S R K Jakka and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing eCry3A mCry3A

and eCrymCry3A stacked genes Journal of Economic Entomology (In preparation)

33 Jakka S R K R B Shrestha and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing Cry3Bb and

Cry3435Ab Journal of Economic Entomology (In preparation)

RB Shrestha Page 12

January 26 2014

12

34 Shrestha R B M N Parajulee S C Carroll J K Dever M D Arnold and J R

Hagler 2014 Characterization of the intercrop movement behavior of Lygus hesperus

between cotton and alfalfa Environmental Entomology (In preparation)

35 Shrestha R B M N Parajulee L Densmore and M San Francisco 2014

Microsatellite marker based genetic diversity of Lygus hesperus from Texas High Plains

Journal of Insect Science (In preparation)

4 Papers Published in Non-Refereed Proceedings (35 Total)

41 R B Shrestha M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

42 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

43 Parajulee M N R B Shrestha and S C Carroll 2013 Evaluation of organic pesticides

for western flower thrips management in seedling cotton Effect on plant parameters

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

44 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

45 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

46 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

47 M N Parajulee Shrestha R B W O McSpadden and SC Carroll 2011

Development of metapopulation approach for landscape-level Lygus Hesperus

management in Texas pp 220 In Proceedings of World Cotton Research Conference-5

Nov7-11 2011 Mumbai India

48 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2011 Evaluation

of varietal differences in COTMAN compensation capacity values in high-input cotton

subjected to varying levels of Lygus-induced fruit damage pp 825-829 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

RB Shrestha Page 13

January 26 2014

13

49 Parajulee M N M B Adhikari D L Kerns R B Shrestha and S C Carroll 2011

Insecticide termination rule for western tarnished plant bug in the Texas High Plains pp

1204-1208 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

410 Parajulee M N D Neupane R B Shrestha Y Yang and L T Wilson 2011 Effect

of cotton fleahopper density on pre-flower fruit loss and plant biomass in cotton pp 1095-

1099 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

411 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on Lygus hesperus abundance in adjacent cotton field A field marking study pp

853-858 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

412 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains pp 1180-88 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

413 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton pp 1155-1162 Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

414 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton pp 1163-1169 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

415 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults versus nymphs pp 849-852 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

416 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa pp 873-881

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

417 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions pp 1159-1162 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

418 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris pp 1118-1129

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

419 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip irrigated cotton pp 1251-

RB Shrestha Page 14

January 26 2014

14

1256 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

420 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus bugs from

Texas pp 1693-1699 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

421 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains pp 1700-1703

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

422 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus pp 1443-1446 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

423 Albeldantildeo W A J E Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity pp 1495-1497 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

424 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton pp 1767-1773 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

425 Parajulee M N R B Shrestha S C Carroll P L Bommireddy A M Cranmer and

K F Bronson 2005 Variable rate of irrigation water and nitrogen Potential of site-

specific management of cotton aphids pp 1735-1742 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

426 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton pp 1779-1783 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

427 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

cotton arthropod predators to bollworm eggs in the laboratory pp 1652-1659

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

428 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii p 387

Proceedings 15th

International Plant Protection Congress Foreign Language Press

Beijing China

429 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research pp 1918-1922 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

430 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Seasonal abundance patterns of thrips and fleahoppers in Texas High

RB Shrestha Page 15

January 26 2014

15

Plains cotton pp 1375-1380 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

431 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains pp 970-973 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

432 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetle in the laboratory pp 1060-1063 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

433 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in Texas High Plains pp 1370-1371 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

434 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed pp 1537-1539 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

435 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton pp 1372-1374 Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

5 Published Scientific Reports (23 Total)

51 Parajulee M N S C Carroll R B Shrestha J P Bordovsky 2012 Effect of nitrogen

fertilizer on cotton host-plant quality and its impact on arthropod activity Helms TX

2012

52 Parajulee M N R B Shrestha S Carroll and Wayne Keeling 2012 Cotton yield

response to late-season Lygus plant bug infestations as influenced by cultivar x irrigation

level treatments Lamesa TX 2012

53 Parajulee M N R B Shrestha and S C Carroll 2012 Characterization of Cotton Crop

Response to Thrips Injury for Improved Thrips Management in Texas High Plains Cotton

2012 Annual Report Cotton Incorporated Core Program Project Number 12-364

54 Parajulee M N R B Shrestha and S Carroll 2012 Development of Economic

Threshold and Management Recommendations for Lygus in Texas High Plains Cotton

2012 Annual Report (Year 1) Cotton IncorporatedTexas State Support Program Project

Number 12-112TX

55 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluating varietal

difference in COTMAN compensation capacity value in drip irrigated cotton using Lygus-

RB Shrestha Page 16

January 26 2014

16

induced fruit damage 2011 Final report Cotton Incorporated Core Program Project

Number 09-596 pp 1-16

56 Parajulee M N S C Carroll R BShrestha AM Cranmer and J P Bordovsky 2011

Influence of soil nitrogen level on seasonal activity of cotton arthropods and lint yield

under drip irrigation (field 6h) 2011 Helms Research Farm Annual Report p19

57 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluation of

westerntarnished plant bug as a late season pest of cotton 2011 Final report Cotton

IncorporatedState Support Program Project Number 08-305TX pp 1-18

58 Parajulee M N O McSpadden R B Shrestha S Carroll and W Keeling 2011

Cotton fruitingyield compensation after Lygus induced square loss as influenced by

variety x water treatments Lamesa TX 2011 Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 30-31

59 Parajulee M N B Kesey O McSpadden S Carroll R B Shrestha and W Keeling

2010 Effect of cover crop on arthropod population dynamics in subsurface drip irrigated

cotton at AG-CARES Lamesa TX 2010 Agricultural Complex for Advanced Research

and Extension Systems Annual Report pp45-46

510 Parajulee M N O McSpadden D Neupane R B Shrestha S Carroll and W

Keeling 2010 Cotton fruitingyield compensation after Lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2010 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 47-48

511 Parajulee M N R B Shrestha O McSpadden A Bastola D Neupane S Carroll and

W Keeling 2009 Cotton fruitingyield compensation after lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2009 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 46-47

512 Parajulee M N S C Carroll R BShrestha R J Kesey D M Nesmith and J P

Bordovsky 2009 Influence of soil nitrogen level on seasonal activity of cotton arthropods

and lint yield under drip irrigation 2009 Helms Research Farm Annual Report p18

513 Parajulee M N A Bastola R B Shrestha and W Keeling 2009 Evaluation of cotton

boll susceptibility to Lygus bugs as influenced by cultivar x irrigation interaction

AGCARES Lamesa TX 2009Agricultural Complex for Advanced Research and

Extension Systems Annual Report pp 48-49

514 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2004 Influence of soil nitrogen level on seasonal activity of cotton arthropods in drip

irrigation system Helms Research Farm Annual Report p 25

515 Parajulee M N J F Leser R B Shrestha A K Barman and R K Boman 2004

Evaluating the merits of sub-threshold effects of the Bollgard technology on the

economics of bollworm management Helms Research Farm Annual Report p 14

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 10: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 10

January 26 2014

10

Publications and Presentations

1 Peer Reviewed Journal Articles

(15 totals 150 times citied) web httpscholargooglecomcitationsuser=JphkwaMAAAAJamphl=en

11 Shrestha R B and M N Parajulee 2013 Potential cotton aphid Aphis gossypii

population suppression by arthropod predators in upland cotton Journal of Insect Science

20(6)778-788

12 Parajulee M N R B Shrestha J E Slosser and D G Bordovsky 2011 Effects of

skip-row planting pattern and planting date on dryland cotton insect pest abundance and

selected plant parameters Southwestern Entomologist 36 21-39

13 Shrestha R B and M N Parajulee 2010 Effect of tillage and planting date on seasonal

abundance and diversity of predacious ground beetles in cotton Journal of Insect Science

10 (174) 1-16

14 Matis T I M N Parajulee J H Matis and R B Shrestha 2008 A mechanistic model

based analysis of cotton aphid population dynamics data Journal of Agriculture and

Forestry Ecology 10 355-362

15 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Egyptian Journal of Agricultural Research 86 57-81

16 Shrestha R B M N Parajulee O P Perera B E Scheffler and L D Densmore

2007 Characterization of microsatellite loci in the western tarnished plant bug Lygus

hesperus (Knight) (Hemiptera Miridae) Molecular Ecology Notes 7 1342-1344

17 Parajulee M N R B Shrestha and J F Leser 2006 Influence of tillage planting date

and Bt cultivar on seasonal abundance and within-plant distribution patterns of thrips and

cotton fleahoppers in cotton International Journal of Pest Management 52 249-260

18 Parajulee M N R B Shrestha and J F Leser 2006 Dispersion patterns and fixed

precision sequential sampling plans for western flower thrips (Thysanoptera Thripidae)

and cotton fleahoppers (Hemiptera Miridae) in cotton Journal of Economic Entomology

99 568-577

19 Parajulee M N R B Shrestha J F Leser D B Wester and C Blanco 2006

Evaluation of the functional response of selected cotton arthropod predators on bollworm

eggs in the laboratory and effect of temperature on their predation efficiency

Environmental Entomology 35 379-386

110 Chen F G Wu F Ge M N Parajulee and R B Shrestha 2005 Effects of elevated

CO2 and transgenic Bt cotton on plant chemistry performance and feeding of an insect

herbivore the cotton bollworm Entomologia Experimentalis et Applicata 115 341-350

RB Shrestha Page 11

January 26 2014

11

111 Dahal G R B Shrestha and R B Thapa 1997 Species composition and relative

abundance of rice green leafhopper (Nephotettix spp) in different altitude regions of

Nepal International Journal of Pest Management 43(1) 49-58

112 Dahal G A Druka T M Burns L C Villegas Z Fan R B Shrestha and R Hull

1996 Some biological and genomic properties of rice tungro bacilliform badna virus and

rice tungro spherical waika virus from Nepal Annals of Applied Biology 129 (2) 267-

287

113 Pokharel R R G Dahal and R B Shrestha 1994 Association of nematode

populations and virus-like symptoms in papaya Journal of Institute of Agriculture and

Animal Science 15 107-113

114 Dahal G R B Shrestha N K Khatri Z Fan and R Hull 1993 Incidence of virus

diseases of rice in Nepal Journal of Institute of Agriculture and Animal science 14 115-

116

115 Dahal G R R Pokharel and R B Shrestha 1992 A bibliographic database of plant

pathology in Nepal Journal of Institute of Agriculture and Animal Science 13 53-64

2 Papers Published in Refereed Proceedings (2 Total)

21 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) Proceedings World Cotton Research

Conference (CD)

22 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review pp 154 -167

Proceeding of second National Conference of Science and Technology

3 Papers in preparation for submission in Journals (5 total)

31 Shrestha R B S R K Jakka and A J Gassmann 2014 Survivorship of Western Corn

Rootworm problem field population on Bt corn hybrids revealed cross resistance between

Cry3Bb and mCry3A Journal of Economic Entomology (In preparation)

32 Shrestha R B S R K Jakka and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing eCry3A mCry3A

and eCrymCry3A stacked genes Journal of Economic Entomology (In preparation)

33 Jakka S R K R B Shrestha and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing Cry3Bb and

Cry3435Ab Journal of Economic Entomology (In preparation)

RB Shrestha Page 12

January 26 2014

12

34 Shrestha R B M N Parajulee S C Carroll J K Dever M D Arnold and J R

Hagler 2014 Characterization of the intercrop movement behavior of Lygus hesperus

between cotton and alfalfa Environmental Entomology (In preparation)

35 Shrestha R B M N Parajulee L Densmore and M San Francisco 2014

Microsatellite marker based genetic diversity of Lygus hesperus from Texas High Plains

Journal of Insect Science (In preparation)

4 Papers Published in Non-Refereed Proceedings (35 Total)

41 R B Shrestha M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

42 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

43 Parajulee M N R B Shrestha and S C Carroll 2013 Evaluation of organic pesticides

for western flower thrips management in seedling cotton Effect on plant parameters

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

44 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

45 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

46 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

47 M N Parajulee Shrestha R B W O McSpadden and SC Carroll 2011

Development of metapopulation approach for landscape-level Lygus Hesperus

management in Texas pp 220 In Proceedings of World Cotton Research Conference-5

Nov7-11 2011 Mumbai India

48 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2011 Evaluation

of varietal differences in COTMAN compensation capacity values in high-input cotton

subjected to varying levels of Lygus-induced fruit damage pp 825-829 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

RB Shrestha Page 13

January 26 2014

13

49 Parajulee M N M B Adhikari D L Kerns R B Shrestha and S C Carroll 2011

Insecticide termination rule for western tarnished plant bug in the Texas High Plains pp

1204-1208 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

410 Parajulee M N D Neupane R B Shrestha Y Yang and L T Wilson 2011 Effect

of cotton fleahopper density on pre-flower fruit loss and plant biomass in cotton pp 1095-

1099 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

411 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on Lygus hesperus abundance in adjacent cotton field A field marking study pp

853-858 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

412 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains pp 1180-88 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

413 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton pp 1155-1162 Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

414 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton pp 1163-1169 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

415 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults versus nymphs pp 849-852 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

416 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa pp 873-881

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

417 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions pp 1159-1162 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

418 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris pp 1118-1129

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

419 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip irrigated cotton pp 1251-

RB Shrestha Page 14

January 26 2014

14

1256 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

420 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus bugs from

Texas pp 1693-1699 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

421 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains pp 1700-1703

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

422 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus pp 1443-1446 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

423 Albeldantildeo W A J E Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity pp 1495-1497 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

424 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton pp 1767-1773 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

425 Parajulee M N R B Shrestha S C Carroll P L Bommireddy A M Cranmer and

K F Bronson 2005 Variable rate of irrigation water and nitrogen Potential of site-

specific management of cotton aphids pp 1735-1742 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

426 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton pp 1779-1783 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

427 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

cotton arthropod predators to bollworm eggs in the laboratory pp 1652-1659

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

428 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii p 387

Proceedings 15th

International Plant Protection Congress Foreign Language Press

Beijing China

429 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research pp 1918-1922 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

430 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Seasonal abundance patterns of thrips and fleahoppers in Texas High

RB Shrestha Page 15

January 26 2014

15

Plains cotton pp 1375-1380 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

431 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains pp 970-973 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

432 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetle in the laboratory pp 1060-1063 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

433 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in Texas High Plains pp 1370-1371 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

434 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed pp 1537-1539 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

435 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton pp 1372-1374 Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

5 Published Scientific Reports (23 Total)

51 Parajulee M N S C Carroll R B Shrestha J P Bordovsky 2012 Effect of nitrogen

fertilizer on cotton host-plant quality and its impact on arthropod activity Helms TX

2012

52 Parajulee M N R B Shrestha S Carroll and Wayne Keeling 2012 Cotton yield

response to late-season Lygus plant bug infestations as influenced by cultivar x irrigation

level treatments Lamesa TX 2012

53 Parajulee M N R B Shrestha and S C Carroll 2012 Characterization of Cotton Crop

Response to Thrips Injury for Improved Thrips Management in Texas High Plains Cotton

2012 Annual Report Cotton Incorporated Core Program Project Number 12-364

54 Parajulee M N R B Shrestha and S Carroll 2012 Development of Economic

Threshold and Management Recommendations for Lygus in Texas High Plains Cotton

2012 Annual Report (Year 1) Cotton IncorporatedTexas State Support Program Project

Number 12-112TX

55 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluating varietal

difference in COTMAN compensation capacity value in drip irrigated cotton using Lygus-

RB Shrestha Page 16

January 26 2014

16

induced fruit damage 2011 Final report Cotton Incorporated Core Program Project

Number 09-596 pp 1-16

56 Parajulee M N S C Carroll R BShrestha AM Cranmer and J P Bordovsky 2011

Influence of soil nitrogen level on seasonal activity of cotton arthropods and lint yield

under drip irrigation (field 6h) 2011 Helms Research Farm Annual Report p19

57 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluation of

westerntarnished plant bug as a late season pest of cotton 2011 Final report Cotton

IncorporatedState Support Program Project Number 08-305TX pp 1-18

58 Parajulee M N O McSpadden R B Shrestha S Carroll and W Keeling 2011

Cotton fruitingyield compensation after Lygus induced square loss as influenced by

variety x water treatments Lamesa TX 2011 Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 30-31

59 Parajulee M N B Kesey O McSpadden S Carroll R B Shrestha and W Keeling

2010 Effect of cover crop on arthropod population dynamics in subsurface drip irrigated

cotton at AG-CARES Lamesa TX 2010 Agricultural Complex for Advanced Research

and Extension Systems Annual Report pp45-46

510 Parajulee M N O McSpadden D Neupane R B Shrestha S Carroll and W

Keeling 2010 Cotton fruitingyield compensation after Lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2010 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 47-48

511 Parajulee M N R B Shrestha O McSpadden A Bastola D Neupane S Carroll and

W Keeling 2009 Cotton fruitingyield compensation after lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2009 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 46-47

512 Parajulee M N S C Carroll R BShrestha R J Kesey D M Nesmith and J P

Bordovsky 2009 Influence of soil nitrogen level on seasonal activity of cotton arthropods

and lint yield under drip irrigation 2009 Helms Research Farm Annual Report p18

513 Parajulee M N A Bastola R B Shrestha and W Keeling 2009 Evaluation of cotton

boll susceptibility to Lygus bugs as influenced by cultivar x irrigation interaction

AGCARES Lamesa TX 2009Agricultural Complex for Advanced Research and

Extension Systems Annual Report pp 48-49

514 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2004 Influence of soil nitrogen level on seasonal activity of cotton arthropods in drip

irrigation system Helms Research Farm Annual Report p 25

515 Parajulee M N J F Leser R B Shrestha A K Barman and R K Boman 2004

Evaluating the merits of sub-threshold effects of the Bollgard technology on the

economics of bollworm management Helms Research Farm Annual Report p 14

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 11: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 11

January 26 2014

11

111 Dahal G R B Shrestha and R B Thapa 1997 Species composition and relative

abundance of rice green leafhopper (Nephotettix spp) in different altitude regions of

Nepal International Journal of Pest Management 43(1) 49-58

112 Dahal G A Druka T M Burns L C Villegas Z Fan R B Shrestha and R Hull

1996 Some biological and genomic properties of rice tungro bacilliform badna virus and

rice tungro spherical waika virus from Nepal Annals of Applied Biology 129 (2) 267-

287

113 Pokharel R R G Dahal and R B Shrestha 1994 Association of nematode

populations and virus-like symptoms in papaya Journal of Institute of Agriculture and

Animal Science 15 107-113

114 Dahal G R B Shrestha N K Khatri Z Fan and R Hull 1993 Incidence of virus

diseases of rice in Nepal Journal of Institute of Agriculture and Animal science 14 115-

116

115 Dahal G R R Pokharel and R B Shrestha 1992 A bibliographic database of plant

pathology in Nepal Journal of Institute of Agriculture and Animal Science 13 53-64

2 Papers Published in Refereed Proceedings (2 Total)

21 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) Proceedings World Cotton Research

Conference (CD)

22 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review pp 154 -167

Proceeding of second National Conference of Science and Technology

3 Papers in preparation for submission in Journals (5 total)

31 Shrestha R B S R K Jakka and A J Gassmann 2014 Survivorship of Western Corn

Rootworm problem field population on Bt corn hybrids revealed cross resistance between

Cry3Bb and mCry3A Journal of Economic Entomology (In preparation)

32 Shrestha R B S R K Jakka and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing eCry3A mCry3A

and eCrymCry3A stacked genes Journal of Economic Entomology (In preparation)

33 Jakka S R K R B Shrestha and A J Gassmann 2014 Evaluation of Western Corn

Rootworm problem field populations against corn hybrids expressing Cry3Bb and

Cry3435Ab Journal of Economic Entomology (In preparation)

RB Shrestha Page 12

January 26 2014

12

34 Shrestha R B M N Parajulee S C Carroll J K Dever M D Arnold and J R

Hagler 2014 Characterization of the intercrop movement behavior of Lygus hesperus

between cotton and alfalfa Environmental Entomology (In preparation)

35 Shrestha R B M N Parajulee L Densmore and M San Francisco 2014

Microsatellite marker based genetic diversity of Lygus hesperus from Texas High Plains

Journal of Insect Science (In preparation)

4 Papers Published in Non-Refereed Proceedings (35 Total)

41 R B Shrestha M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

42 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

43 Parajulee M N R B Shrestha and S C Carroll 2013 Evaluation of organic pesticides

for western flower thrips management in seedling cotton Effect on plant parameters

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

44 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

45 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

46 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

47 M N Parajulee Shrestha R B W O McSpadden and SC Carroll 2011

Development of metapopulation approach for landscape-level Lygus Hesperus

management in Texas pp 220 In Proceedings of World Cotton Research Conference-5

Nov7-11 2011 Mumbai India

48 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2011 Evaluation

of varietal differences in COTMAN compensation capacity values in high-input cotton

subjected to varying levels of Lygus-induced fruit damage pp 825-829 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

RB Shrestha Page 13

January 26 2014

13

49 Parajulee M N M B Adhikari D L Kerns R B Shrestha and S C Carroll 2011

Insecticide termination rule for western tarnished plant bug in the Texas High Plains pp

1204-1208 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

410 Parajulee M N D Neupane R B Shrestha Y Yang and L T Wilson 2011 Effect

of cotton fleahopper density on pre-flower fruit loss and plant biomass in cotton pp 1095-

1099 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

411 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on Lygus hesperus abundance in adjacent cotton field A field marking study pp

853-858 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

412 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains pp 1180-88 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

413 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton pp 1155-1162 Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

414 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton pp 1163-1169 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

415 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults versus nymphs pp 849-852 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

416 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa pp 873-881

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

417 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions pp 1159-1162 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

418 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris pp 1118-1129

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

419 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip irrigated cotton pp 1251-

RB Shrestha Page 14

January 26 2014

14

1256 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

420 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus bugs from

Texas pp 1693-1699 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

421 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains pp 1700-1703

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

422 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus pp 1443-1446 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

423 Albeldantildeo W A J E Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity pp 1495-1497 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

424 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton pp 1767-1773 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

425 Parajulee M N R B Shrestha S C Carroll P L Bommireddy A M Cranmer and

K F Bronson 2005 Variable rate of irrigation water and nitrogen Potential of site-

specific management of cotton aphids pp 1735-1742 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

426 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton pp 1779-1783 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

427 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

cotton arthropod predators to bollworm eggs in the laboratory pp 1652-1659

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

428 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii p 387

Proceedings 15th

International Plant Protection Congress Foreign Language Press

Beijing China

429 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research pp 1918-1922 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

430 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Seasonal abundance patterns of thrips and fleahoppers in Texas High

RB Shrestha Page 15

January 26 2014

15

Plains cotton pp 1375-1380 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

431 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains pp 970-973 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

432 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetle in the laboratory pp 1060-1063 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

433 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in Texas High Plains pp 1370-1371 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

434 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed pp 1537-1539 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

435 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton pp 1372-1374 Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

5 Published Scientific Reports (23 Total)

51 Parajulee M N S C Carroll R B Shrestha J P Bordovsky 2012 Effect of nitrogen

fertilizer on cotton host-plant quality and its impact on arthropod activity Helms TX

2012

52 Parajulee M N R B Shrestha S Carroll and Wayne Keeling 2012 Cotton yield

response to late-season Lygus plant bug infestations as influenced by cultivar x irrigation

level treatments Lamesa TX 2012

53 Parajulee M N R B Shrestha and S C Carroll 2012 Characterization of Cotton Crop

Response to Thrips Injury for Improved Thrips Management in Texas High Plains Cotton

2012 Annual Report Cotton Incorporated Core Program Project Number 12-364

54 Parajulee M N R B Shrestha and S Carroll 2012 Development of Economic

Threshold and Management Recommendations for Lygus in Texas High Plains Cotton

2012 Annual Report (Year 1) Cotton IncorporatedTexas State Support Program Project

Number 12-112TX

55 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluating varietal

difference in COTMAN compensation capacity value in drip irrigated cotton using Lygus-

RB Shrestha Page 16

January 26 2014

16

induced fruit damage 2011 Final report Cotton Incorporated Core Program Project

Number 09-596 pp 1-16

56 Parajulee M N S C Carroll R BShrestha AM Cranmer and J P Bordovsky 2011

Influence of soil nitrogen level on seasonal activity of cotton arthropods and lint yield

under drip irrigation (field 6h) 2011 Helms Research Farm Annual Report p19

57 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluation of

westerntarnished plant bug as a late season pest of cotton 2011 Final report Cotton

IncorporatedState Support Program Project Number 08-305TX pp 1-18

58 Parajulee M N O McSpadden R B Shrestha S Carroll and W Keeling 2011

Cotton fruitingyield compensation after Lygus induced square loss as influenced by

variety x water treatments Lamesa TX 2011 Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 30-31

59 Parajulee M N B Kesey O McSpadden S Carroll R B Shrestha and W Keeling

2010 Effect of cover crop on arthropod population dynamics in subsurface drip irrigated

cotton at AG-CARES Lamesa TX 2010 Agricultural Complex for Advanced Research

and Extension Systems Annual Report pp45-46

510 Parajulee M N O McSpadden D Neupane R B Shrestha S Carroll and W

Keeling 2010 Cotton fruitingyield compensation after Lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2010 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 47-48

511 Parajulee M N R B Shrestha O McSpadden A Bastola D Neupane S Carroll and

W Keeling 2009 Cotton fruitingyield compensation after lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2009 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 46-47

512 Parajulee M N S C Carroll R BShrestha R J Kesey D M Nesmith and J P

Bordovsky 2009 Influence of soil nitrogen level on seasonal activity of cotton arthropods

and lint yield under drip irrigation 2009 Helms Research Farm Annual Report p18

513 Parajulee M N A Bastola R B Shrestha and W Keeling 2009 Evaluation of cotton

boll susceptibility to Lygus bugs as influenced by cultivar x irrigation interaction

AGCARES Lamesa TX 2009Agricultural Complex for Advanced Research and

Extension Systems Annual Report pp 48-49

514 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2004 Influence of soil nitrogen level on seasonal activity of cotton arthropods in drip

irrigation system Helms Research Farm Annual Report p 25

515 Parajulee M N J F Leser R B Shrestha A K Barman and R K Boman 2004

Evaluating the merits of sub-threshold effects of the Bollgard technology on the

economics of bollworm management Helms Research Farm Annual Report p 14

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 12: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 12

January 26 2014

12

34 Shrestha R B M N Parajulee S C Carroll J K Dever M D Arnold and J R

Hagler 2014 Characterization of the intercrop movement behavior of Lygus hesperus

between cotton and alfalfa Environmental Entomology (In preparation)

35 Shrestha R B M N Parajulee L Densmore and M San Francisco 2014

Microsatellite marker based genetic diversity of Lygus hesperus from Texas High Plains

Journal of Insect Science (In preparation)

4 Papers Published in Non-Refereed Proceedings (35 Total)

41 R B Shrestha M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

42 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

43 Parajulee M N R B Shrestha and S C Carroll 2013 Evaluation of organic pesticides

for western flower thrips management in seedling cotton Effect on plant parameters

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

44 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

45 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

46 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

47 M N Parajulee Shrestha R B W O McSpadden and SC Carroll 2011

Development of metapopulation approach for landscape-level Lygus Hesperus

management in Texas pp 220 In Proceedings of World Cotton Research Conference-5

Nov7-11 2011 Mumbai India

48 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2011 Evaluation

of varietal differences in COTMAN compensation capacity values in high-input cotton

subjected to varying levels of Lygus-induced fruit damage pp 825-829 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

RB Shrestha Page 13

January 26 2014

13

49 Parajulee M N M B Adhikari D L Kerns R B Shrestha and S C Carroll 2011

Insecticide termination rule for western tarnished plant bug in the Texas High Plains pp

1204-1208 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

410 Parajulee M N D Neupane R B Shrestha Y Yang and L T Wilson 2011 Effect

of cotton fleahopper density on pre-flower fruit loss and plant biomass in cotton pp 1095-

1099 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

411 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on Lygus hesperus abundance in adjacent cotton field A field marking study pp

853-858 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

412 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains pp 1180-88 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

413 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton pp 1155-1162 Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

414 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton pp 1163-1169 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

415 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults versus nymphs pp 849-852 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

416 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa pp 873-881

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

417 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions pp 1159-1162 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

418 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris pp 1118-1129

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

419 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip irrigated cotton pp 1251-

RB Shrestha Page 14

January 26 2014

14

1256 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

420 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus bugs from

Texas pp 1693-1699 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

421 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains pp 1700-1703

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

422 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus pp 1443-1446 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

423 Albeldantildeo W A J E Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity pp 1495-1497 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

424 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton pp 1767-1773 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

425 Parajulee M N R B Shrestha S C Carroll P L Bommireddy A M Cranmer and

K F Bronson 2005 Variable rate of irrigation water and nitrogen Potential of site-

specific management of cotton aphids pp 1735-1742 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

426 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton pp 1779-1783 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

427 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

cotton arthropod predators to bollworm eggs in the laboratory pp 1652-1659

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

428 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii p 387

Proceedings 15th

International Plant Protection Congress Foreign Language Press

Beijing China

429 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research pp 1918-1922 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

430 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Seasonal abundance patterns of thrips and fleahoppers in Texas High

RB Shrestha Page 15

January 26 2014

15

Plains cotton pp 1375-1380 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

431 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains pp 970-973 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

432 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetle in the laboratory pp 1060-1063 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

433 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in Texas High Plains pp 1370-1371 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

434 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed pp 1537-1539 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

435 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton pp 1372-1374 Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

5 Published Scientific Reports (23 Total)

51 Parajulee M N S C Carroll R B Shrestha J P Bordovsky 2012 Effect of nitrogen

fertilizer on cotton host-plant quality and its impact on arthropod activity Helms TX

2012

52 Parajulee M N R B Shrestha S Carroll and Wayne Keeling 2012 Cotton yield

response to late-season Lygus plant bug infestations as influenced by cultivar x irrigation

level treatments Lamesa TX 2012

53 Parajulee M N R B Shrestha and S C Carroll 2012 Characterization of Cotton Crop

Response to Thrips Injury for Improved Thrips Management in Texas High Plains Cotton

2012 Annual Report Cotton Incorporated Core Program Project Number 12-364

54 Parajulee M N R B Shrestha and S Carroll 2012 Development of Economic

Threshold and Management Recommendations for Lygus in Texas High Plains Cotton

2012 Annual Report (Year 1) Cotton IncorporatedTexas State Support Program Project

Number 12-112TX

55 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluating varietal

difference in COTMAN compensation capacity value in drip irrigated cotton using Lygus-

RB Shrestha Page 16

January 26 2014

16

induced fruit damage 2011 Final report Cotton Incorporated Core Program Project

Number 09-596 pp 1-16

56 Parajulee M N S C Carroll R BShrestha AM Cranmer and J P Bordovsky 2011

Influence of soil nitrogen level on seasonal activity of cotton arthropods and lint yield

under drip irrigation (field 6h) 2011 Helms Research Farm Annual Report p19

57 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluation of

westerntarnished plant bug as a late season pest of cotton 2011 Final report Cotton

IncorporatedState Support Program Project Number 08-305TX pp 1-18

58 Parajulee M N O McSpadden R B Shrestha S Carroll and W Keeling 2011

Cotton fruitingyield compensation after Lygus induced square loss as influenced by

variety x water treatments Lamesa TX 2011 Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 30-31

59 Parajulee M N B Kesey O McSpadden S Carroll R B Shrestha and W Keeling

2010 Effect of cover crop on arthropod population dynamics in subsurface drip irrigated

cotton at AG-CARES Lamesa TX 2010 Agricultural Complex for Advanced Research

and Extension Systems Annual Report pp45-46

510 Parajulee M N O McSpadden D Neupane R B Shrestha S Carroll and W

Keeling 2010 Cotton fruitingyield compensation after Lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2010 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 47-48

511 Parajulee M N R B Shrestha O McSpadden A Bastola D Neupane S Carroll and

W Keeling 2009 Cotton fruitingyield compensation after lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2009 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 46-47

512 Parajulee M N S C Carroll R BShrestha R J Kesey D M Nesmith and J P

Bordovsky 2009 Influence of soil nitrogen level on seasonal activity of cotton arthropods

and lint yield under drip irrigation 2009 Helms Research Farm Annual Report p18

513 Parajulee M N A Bastola R B Shrestha and W Keeling 2009 Evaluation of cotton

boll susceptibility to Lygus bugs as influenced by cultivar x irrigation interaction

AGCARES Lamesa TX 2009Agricultural Complex for Advanced Research and

Extension Systems Annual Report pp 48-49

514 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2004 Influence of soil nitrogen level on seasonal activity of cotton arthropods in drip

irrigation system Helms Research Farm Annual Report p 25

515 Parajulee M N J F Leser R B Shrestha A K Barman and R K Boman 2004

Evaluating the merits of sub-threshold effects of the Bollgard technology on the

economics of bollworm management Helms Research Farm Annual Report p 14

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 13: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 13

January 26 2014

13

49 Parajulee M N M B Adhikari D L Kerns R B Shrestha and S C Carroll 2011

Insecticide termination rule for western tarnished plant bug in the Texas High Plains pp

1204-1208 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

410 Parajulee M N D Neupane R B Shrestha Y Yang and L T Wilson 2011 Effect

of cotton fleahopper density on pre-flower fruit loss and plant biomass in cotton pp 1095-

1099 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

411 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on Lygus hesperus abundance in adjacent cotton field A field marking study pp

853-858 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

412 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains pp 1180-88 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

413 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton pp 1155-1162 Proceedings Beltwide

Cotton Conferences National Cotton Council Memphis TN

414 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton pp 1163-1169 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

415 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults versus nymphs pp 849-852 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

416 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa pp 873-881

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

417 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions pp 1159-1162 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

418 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris pp 1118-1129

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

419 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip irrigated cotton pp 1251-

RB Shrestha Page 14

January 26 2014

14

1256 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

420 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus bugs from

Texas pp 1693-1699 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

421 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains pp 1700-1703

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

422 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus pp 1443-1446 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

423 Albeldantildeo W A J E Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity pp 1495-1497 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

424 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton pp 1767-1773 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

425 Parajulee M N R B Shrestha S C Carroll P L Bommireddy A M Cranmer and

K F Bronson 2005 Variable rate of irrigation water and nitrogen Potential of site-

specific management of cotton aphids pp 1735-1742 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

426 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton pp 1779-1783 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

427 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

cotton arthropod predators to bollworm eggs in the laboratory pp 1652-1659

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

428 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii p 387

Proceedings 15th

International Plant Protection Congress Foreign Language Press

Beijing China

429 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research pp 1918-1922 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

430 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Seasonal abundance patterns of thrips and fleahoppers in Texas High

RB Shrestha Page 15

January 26 2014

15

Plains cotton pp 1375-1380 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

431 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains pp 970-973 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

432 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetle in the laboratory pp 1060-1063 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

433 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in Texas High Plains pp 1370-1371 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

434 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed pp 1537-1539 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

435 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton pp 1372-1374 Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

5 Published Scientific Reports (23 Total)

51 Parajulee M N S C Carroll R B Shrestha J P Bordovsky 2012 Effect of nitrogen

fertilizer on cotton host-plant quality and its impact on arthropod activity Helms TX

2012

52 Parajulee M N R B Shrestha S Carroll and Wayne Keeling 2012 Cotton yield

response to late-season Lygus plant bug infestations as influenced by cultivar x irrigation

level treatments Lamesa TX 2012

53 Parajulee M N R B Shrestha and S C Carroll 2012 Characterization of Cotton Crop

Response to Thrips Injury for Improved Thrips Management in Texas High Plains Cotton

2012 Annual Report Cotton Incorporated Core Program Project Number 12-364

54 Parajulee M N R B Shrestha and S Carroll 2012 Development of Economic

Threshold and Management Recommendations for Lygus in Texas High Plains Cotton

2012 Annual Report (Year 1) Cotton IncorporatedTexas State Support Program Project

Number 12-112TX

55 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluating varietal

difference in COTMAN compensation capacity value in drip irrigated cotton using Lygus-

RB Shrestha Page 16

January 26 2014

16

induced fruit damage 2011 Final report Cotton Incorporated Core Program Project

Number 09-596 pp 1-16

56 Parajulee M N S C Carroll R BShrestha AM Cranmer and J P Bordovsky 2011

Influence of soil nitrogen level on seasonal activity of cotton arthropods and lint yield

under drip irrigation (field 6h) 2011 Helms Research Farm Annual Report p19

57 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluation of

westerntarnished plant bug as a late season pest of cotton 2011 Final report Cotton

IncorporatedState Support Program Project Number 08-305TX pp 1-18

58 Parajulee M N O McSpadden R B Shrestha S Carroll and W Keeling 2011

Cotton fruitingyield compensation after Lygus induced square loss as influenced by

variety x water treatments Lamesa TX 2011 Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 30-31

59 Parajulee M N B Kesey O McSpadden S Carroll R B Shrestha and W Keeling

2010 Effect of cover crop on arthropod population dynamics in subsurface drip irrigated

cotton at AG-CARES Lamesa TX 2010 Agricultural Complex for Advanced Research

and Extension Systems Annual Report pp45-46

510 Parajulee M N O McSpadden D Neupane R B Shrestha S Carroll and W

Keeling 2010 Cotton fruitingyield compensation after Lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2010 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 47-48

511 Parajulee M N R B Shrestha O McSpadden A Bastola D Neupane S Carroll and

W Keeling 2009 Cotton fruitingyield compensation after lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2009 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 46-47

512 Parajulee M N S C Carroll R BShrestha R J Kesey D M Nesmith and J P

Bordovsky 2009 Influence of soil nitrogen level on seasonal activity of cotton arthropods

and lint yield under drip irrigation 2009 Helms Research Farm Annual Report p18

513 Parajulee M N A Bastola R B Shrestha and W Keeling 2009 Evaluation of cotton

boll susceptibility to Lygus bugs as influenced by cultivar x irrigation interaction

AGCARES Lamesa TX 2009Agricultural Complex for Advanced Research and

Extension Systems Annual Report pp 48-49

514 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2004 Influence of soil nitrogen level on seasonal activity of cotton arthropods in drip

irrigation system Helms Research Farm Annual Report p 25

515 Parajulee M N J F Leser R B Shrestha A K Barman and R K Boman 2004

Evaluating the merits of sub-threshold effects of the Bollgard technology on the

economics of bollworm management Helms Research Farm Annual Report p 14

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 14: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 14

January 26 2014

14

1256 Proceedings Beltwide Cotton Conferences National Cotton Council Memphis

TN

420 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus bugs from

Texas pp 1693-1699 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

421 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains pp 1700-1703

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

422 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus pp 1443-1446 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

423 Albeldantildeo W A J E Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity pp 1495-1497 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

424 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton pp 1767-1773 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

425 Parajulee M N R B Shrestha S C Carroll P L Bommireddy A M Cranmer and

K F Bronson 2005 Variable rate of irrigation water and nitrogen Potential of site-

specific management of cotton aphids pp 1735-1742 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

426 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton pp 1779-1783 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

427 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

cotton arthropod predators to bollworm eggs in the laboratory pp 1652-1659

Proceedings Beltwide Cotton Conferences National Cotton Council Memphis TN

428 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii p 387

Proceedings 15th

International Plant Protection Congress Foreign Language Press

Beijing China

429 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research pp 1918-1922 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

430 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Seasonal abundance patterns of thrips and fleahoppers in Texas High

RB Shrestha Page 15

January 26 2014

15

Plains cotton pp 1375-1380 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

431 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains pp 970-973 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

432 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetle in the laboratory pp 1060-1063 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

433 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in Texas High Plains pp 1370-1371 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

434 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed pp 1537-1539 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

435 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton pp 1372-1374 Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

5 Published Scientific Reports (23 Total)

51 Parajulee M N S C Carroll R B Shrestha J P Bordovsky 2012 Effect of nitrogen

fertilizer on cotton host-plant quality and its impact on arthropod activity Helms TX

2012

52 Parajulee M N R B Shrestha S Carroll and Wayne Keeling 2012 Cotton yield

response to late-season Lygus plant bug infestations as influenced by cultivar x irrigation

level treatments Lamesa TX 2012

53 Parajulee M N R B Shrestha and S C Carroll 2012 Characterization of Cotton Crop

Response to Thrips Injury for Improved Thrips Management in Texas High Plains Cotton

2012 Annual Report Cotton Incorporated Core Program Project Number 12-364

54 Parajulee M N R B Shrestha and S Carroll 2012 Development of Economic

Threshold and Management Recommendations for Lygus in Texas High Plains Cotton

2012 Annual Report (Year 1) Cotton IncorporatedTexas State Support Program Project

Number 12-112TX

55 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluating varietal

difference in COTMAN compensation capacity value in drip irrigated cotton using Lygus-

RB Shrestha Page 16

January 26 2014

16

induced fruit damage 2011 Final report Cotton Incorporated Core Program Project

Number 09-596 pp 1-16

56 Parajulee M N S C Carroll R BShrestha AM Cranmer and J P Bordovsky 2011

Influence of soil nitrogen level on seasonal activity of cotton arthropods and lint yield

under drip irrigation (field 6h) 2011 Helms Research Farm Annual Report p19

57 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluation of

westerntarnished plant bug as a late season pest of cotton 2011 Final report Cotton

IncorporatedState Support Program Project Number 08-305TX pp 1-18

58 Parajulee M N O McSpadden R B Shrestha S Carroll and W Keeling 2011

Cotton fruitingyield compensation after Lygus induced square loss as influenced by

variety x water treatments Lamesa TX 2011 Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 30-31

59 Parajulee M N B Kesey O McSpadden S Carroll R B Shrestha and W Keeling

2010 Effect of cover crop on arthropod population dynamics in subsurface drip irrigated

cotton at AG-CARES Lamesa TX 2010 Agricultural Complex for Advanced Research

and Extension Systems Annual Report pp45-46

510 Parajulee M N O McSpadden D Neupane R B Shrestha S Carroll and W

Keeling 2010 Cotton fruitingyield compensation after Lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2010 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 47-48

511 Parajulee M N R B Shrestha O McSpadden A Bastola D Neupane S Carroll and

W Keeling 2009 Cotton fruitingyield compensation after lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2009 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 46-47

512 Parajulee M N S C Carroll R BShrestha R J Kesey D M Nesmith and J P

Bordovsky 2009 Influence of soil nitrogen level on seasonal activity of cotton arthropods

and lint yield under drip irrigation 2009 Helms Research Farm Annual Report p18

513 Parajulee M N A Bastola R B Shrestha and W Keeling 2009 Evaluation of cotton

boll susceptibility to Lygus bugs as influenced by cultivar x irrigation interaction

AGCARES Lamesa TX 2009Agricultural Complex for Advanced Research and

Extension Systems Annual Report pp 48-49

514 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2004 Influence of soil nitrogen level on seasonal activity of cotton arthropods in drip

irrigation system Helms Research Farm Annual Report p 25

515 Parajulee M N J F Leser R B Shrestha A K Barman and R K Boman 2004

Evaluating the merits of sub-threshold effects of the Bollgard technology on the

economics of bollworm management Helms Research Farm Annual Report p 14

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 15: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 15

January 26 2014

15

Plains cotton pp 1375-1380 Proceedings Beltwide Cotton Conferences National Cotton

Council Memphis TN

431 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains pp 970-973 Proceedings Beltwide Cotton Conferences National Cotton Council

Memphis TN

432 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetle in the laboratory pp 1060-1063 Proceedings Beltwide Cotton

Conferences National Cotton Council Memphis TN

433 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in Texas High Plains pp 1370-1371 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

434 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed pp 1537-1539 Proceedings

Beltwide Cotton Conferences National Cotton Council Memphis TN

435 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton pp 1372-1374 Proceedings Beltwide Cotton Conferences

National Cotton Council Memphis TN

5 Published Scientific Reports (23 Total)

51 Parajulee M N S C Carroll R B Shrestha J P Bordovsky 2012 Effect of nitrogen

fertilizer on cotton host-plant quality and its impact on arthropod activity Helms TX

2012

52 Parajulee M N R B Shrestha S Carroll and Wayne Keeling 2012 Cotton yield

response to late-season Lygus plant bug infestations as influenced by cultivar x irrigation

level treatments Lamesa TX 2012

53 Parajulee M N R B Shrestha and S C Carroll 2012 Characterization of Cotton Crop

Response to Thrips Injury for Improved Thrips Management in Texas High Plains Cotton

2012 Annual Report Cotton Incorporated Core Program Project Number 12-364

54 Parajulee M N R B Shrestha and S Carroll 2012 Development of Economic

Threshold and Management Recommendations for Lygus in Texas High Plains Cotton

2012 Annual Report (Year 1) Cotton IncorporatedTexas State Support Program Project

Number 12-112TX

55 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluating varietal

difference in COTMAN compensation capacity value in drip irrigated cotton using Lygus-

RB Shrestha Page 16

January 26 2014

16

induced fruit damage 2011 Final report Cotton Incorporated Core Program Project

Number 09-596 pp 1-16

56 Parajulee M N S C Carroll R BShrestha AM Cranmer and J P Bordovsky 2011

Influence of soil nitrogen level on seasonal activity of cotton arthropods and lint yield

under drip irrigation (field 6h) 2011 Helms Research Farm Annual Report p19

57 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluation of

westerntarnished plant bug as a late season pest of cotton 2011 Final report Cotton

IncorporatedState Support Program Project Number 08-305TX pp 1-18

58 Parajulee M N O McSpadden R B Shrestha S Carroll and W Keeling 2011

Cotton fruitingyield compensation after Lygus induced square loss as influenced by

variety x water treatments Lamesa TX 2011 Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 30-31

59 Parajulee M N B Kesey O McSpadden S Carroll R B Shrestha and W Keeling

2010 Effect of cover crop on arthropod population dynamics in subsurface drip irrigated

cotton at AG-CARES Lamesa TX 2010 Agricultural Complex for Advanced Research

and Extension Systems Annual Report pp45-46

510 Parajulee M N O McSpadden D Neupane R B Shrestha S Carroll and W

Keeling 2010 Cotton fruitingyield compensation after Lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2010 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 47-48

511 Parajulee M N R B Shrestha O McSpadden A Bastola D Neupane S Carroll and

W Keeling 2009 Cotton fruitingyield compensation after lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2009 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 46-47

512 Parajulee M N S C Carroll R BShrestha R J Kesey D M Nesmith and J P

Bordovsky 2009 Influence of soil nitrogen level on seasonal activity of cotton arthropods

and lint yield under drip irrigation 2009 Helms Research Farm Annual Report p18

513 Parajulee M N A Bastola R B Shrestha and W Keeling 2009 Evaluation of cotton

boll susceptibility to Lygus bugs as influenced by cultivar x irrigation interaction

AGCARES Lamesa TX 2009Agricultural Complex for Advanced Research and

Extension Systems Annual Report pp 48-49

514 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2004 Influence of soil nitrogen level on seasonal activity of cotton arthropods in drip

irrigation system Helms Research Farm Annual Report p 25

515 Parajulee M N J F Leser R B Shrestha A K Barman and R K Boman 2004

Evaluating the merits of sub-threshold effects of the Bollgard technology on the

economics of bollworm management Helms Research Farm Annual Report p 14

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 16: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 16

January 26 2014

16

induced fruit damage 2011 Final report Cotton Incorporated Core Program Project

Number 09-596 pp 1-16

56 Parajulee M N S C Carroll R BShrestha AM Cranmer and J P Bordovsky 2011

Influence of soil nitrogen level on seasonal activity of cotton arthropods and lint yield

under drip irrigation (field 6h) 2011 Helms Research Farm Annual Report p19

57 Parajulee M N R B Shrestha O McSpadden S Carroll 2011 Evaluation of

westerntarnished plant bug as a late season pest of cotton 2011 Final report Cotton

IncorporatedState Support Program Project Number 08-305TX pp 1-18

58 Parajulee M N O McSpadden R B Shrestha S Carroll and W Keeling 2011

Cotton fruitingyield compensation after Lygus induced square loss as influenced by

variety x water treatments Lamesa TX 2011 Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 30-31

59 Parajulee M N B Kesey O McSpadden S Carroll R B Shrestha and W Keeling

2010 Effect of cover crop on arthropod population dynamics in subsurface drip irrigated

cotton at AG-CARES Lamesa TX 2010 Agricultural Complex for Advanced Research

and Extension Systems Annual Report pp45-46

510 Parajulee M N O McSpadden D Neupane R B Shrestha S Carroll and W

Keeling 2010 Cotton fruitingyield compensation after Lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2010 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 47-48

511 Parajulee M N R B Shrestha O McSpadden A Bastola D Neupane S Carroll and

W Keeling 2009 Cotton fruitingyield compensation after lygus induced square loss as

influenced by variety x water treatments Lamesa TX 2009 Agricultural Complex for

Advanced Research and Extension Systems Annual Report pp 46-47

512 Parajulee M N S C Carroll R BShrestha R J Kesey D M Nesmith and J P

Bordovsky 2009 Influence of soil nitrogen level on seasonal activity of cotton arthropods

and lint yield under drip irrigation 2009 Helms Research Farm Annual Report p18

513 Parajulee M N A Bastola R B Shrestha and W Keeling 2009 Evaluation of cotton

boll susceptibility to Lygus bugs as influenced by cultivar x irrigation interaction

AGCARES Lamesa TX 2009Agricultural Complex for Advanced Research and

Extension Systems Annual Report pp 48-49

514 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2004 Influence of soil nitrogen level on seasonal activity of cotton arthropods in drip

irrigation system Helms Research Farm Annual Report p 25

515 Parajulee M N J F Leser R B Shrestha A K Barman and R K Boman 2004

Evaluating the merits of sub-threshold effects of the Bollgard technology on the

economics of bollworm management Helms Research Farm Annual Report p 14

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 17: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 17

January 26 2014

17

516 Parajulee M N A K Barman R B Shrestha S C Carroll D M Nesmith and J P

Bordovsky 2004 Influence of cotton cultivar and planting date on Lygus populations

Helms Research Farm Annual Report p 13

517 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2003 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 42-45

518 Parajulee M N R B Shrestha S C Carroll D M Nesmith and J P Bordovsky

2003 Influence of soil nitrogen level on seasonal activity of cotton arthropods with drip

irrigation systems Helms Research Farm Annual Report p 23

519 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll D M Nesmith and J P Bordovsky 2003 Influence of cotton cultivar and

planting date on arthropod populations Helms Research Farm Annual Report p 12

520 Parajulee M N P L Bommireddy D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2002 Influence of irrigation regimes on insect populations in

Texas High Plains cotton Western Peanut Growers Research Farm Annual Report

521 Parajulee M N R B Shrestha S C Carroll M D Arnold A M Cranmer and P L

Bommireddy 2002 Cotton insect pests and natural enemies in conventional and

conservation tillage systems at AG-CARES Lamesa Agricultural Complex for Advanced

Research and Extension Systems Annual Report pp 32-41

522 Parajulee M N R B Shrestha S C Carroll and J P Bordovsky 2002 Influence of

three irrigation methods (LEPA Overhead Spray and Drip) on seasonal activity of cotton

arthropods Helms Research Farm Annual Report p 11

523 Parajulee M N A M Cranmer J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2002 Influence of cotton cultivar and planting date on

arthropod populations Helms Research Farm Annual Report p 10

6 Oral Presentations (55 Total)

61 International meetings invited symposium talks (total 9)

611 Parajulee MN D Zhaoke F Ge and R B Shrestha 2012 Climate Change and Pest

Management Horizontal and Vertical Distribution of Existing Pests as Result of Climate

Change and Possible Modifications in Management Strategies Invited keynote talk in

International Symposium on Food Security Dilemma Plant Health and Climate Change

Issues FTC (Lake Hall) Kalyani West Bengal India (7-9 December)

612 Shrestha R B and S C Carroll and M N Parajulee Development of Lygus

management strategies for Texas cotton 3rd

International Lygus Symposium Scottsdale

Arizona the USA (28-31 October )

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 18: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 18

January 26 2014

18

613 Parajulee M N R B Shrestha and S C Carroll Cotton plant response to selected

Lygus infestation levels in the Texas High Plains 3rd

International Lygus Symposium

Scottsdale Arizona the USA (28-31 October )

614 R B Shrestha and Parajulee M N 2012 Influence of transgenic Bt-cotton on

predacious arthropod assemblage and their impact on non-target pest suppression in

Texas 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

615 Parajulee M N R B Shrestha2012 Landscape-level cultural control as an arthropod

pest management approach in Texas High Plains cotton agro-ecosystems 24th

International Congress of Entomology Daegu Korea (Aug 19-25)

616 Parajulee M N R B Shrestha A K Barman and S C Carroll 2008 Ecologically

intensive pest management in cotton agro-ecosystems Lygus hesperus as a model system

Fourth International Conference of Plant Protection Institute Dokki-Giza Egypt (Nov 9-

12) (Keynote Speech)

617 Shrestha R B M N Parajulee and M J Grimson 2007 SEM ultrastructure study of

Lygus hesperus (Knight) (Hemiptera Miridae) World Cotton Research Conference

Lubbock TX USA (Sept 10-14)

618 Parajulee M N S C Carroll M D Arnold R B Shrestha A M Cranmer and P L

Bommireddy 2005 Species composition and host-plant sequence of Lygus bugs in the

Texas High Plains International Symposium on Ecology and Management of Lygus Plant

Bugs Ottawa Canada (Jan 30-Feb 3)

619 Parajulee M N and R B Shrestha 2004 Biological control of cotton aphids Aphis

gossypii in cotton Effectiveness of Hippodamia convergens and Chrysoperla carnea

The 22nd

International Congress of Entomology Brisbane Australia (Aug 15-21)

62 National meetings invited symposium talks (total 13)

621 Shrestha R B 2012 Past present and future of integrated pest management programs

in developed and developing countries Invited symposium talk at Global Collaboration

for the Development of Next Generation IPM Program 60th Annual Meeting of ESA

November 11-14 2012 in Knoxville TN

622 Yubak G C R B Shrestha and J P Rijal 2012 Experiences from community based

IPM program in Nepal Invited symposium talk at Global Collaboration for the

Development of Next Generation IPM Program 60th Annual Meeting of ESA November

11-14 2012 in Knoxville TN

623 R B Shrestha and MN Parajulee 2012 Lygus management in the Texas High Plains

High Plains Association of Crop Consultants meeting Lubbock TX (Mar 5-7)

624 Shrestha R B M N Parajulee and S C Carroll 2010 Immunological assays in

ecological studies Movement behavior of Lygus bugs in cotton Presented in 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 19: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 19

January 26 2014

19

625 Parajulee M N and R B Shrestha 2010 Quantifying the economic damage potential

of plant bugs in Texas High Plains cotton and developing management recommendations

Presented in 10th

CERIICRC Annual Research and Outreach Symposium at International

Cultural Center Lubbock TX (Apr 6)

626 Parajulee M N W O McSpadden R B Shrestha D L Kerns P C Ellsworth P B

Goodell and S C Carroll 2010 Landscape impacts on Lygus management in West

Texas Presented in 58th

annual meeting of ESA San Diego CA (Dec 12-15)

627 Shrestha R B 2010 Relationships between IMP and molecular biology Invited guest

lecture for Entomology graduate class at Department of Plant and Soil Science Lubbock

TX (Fall 2010)

628 Shrestha R B and M N Parajulee 2009 Challenges and Opportunities in

Identification of Lygus Species from Texas Presented in Entomology Science Conference

Texas AampM University College Station TX (Nov 2-4) (Oral Presentation)

629 Parajulee M N and R B Shrestha 2008 Immunological assays in ecological studies

Movement behavior of Lygus bugs in cotton Presented in 56th

annual meeting of SWB of

ESA Fort Worth TX (Feb 23-26)

6210 Parajulee M N and R B Shrestha 2008 Overview of molecular research programs in

cotton entomology laboratory at the Lubbock Texas Presented in 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

6211 Adhikari M B M N Parajulee R B Shrestha D L Kerns and S C Carroll 2008

Boll susceptibility threshold for Lygus hesperus When is the boll safe Entomology

Science Conference College Station TX (Nov 5-7)

6212 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains High Plains Association of Crop Consultants meeting Lubbock TX (Mar 4-5)

6213 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

seasonal activity in cotton as affected by pigweed High Plains Association of Crop

Consultants annual meeting Lubbock TX (Mar 4-5)

63 National meetings submitted talks (total 33)

631 Shrestha R B and A J Gassmann 2013 Problems and prospects for management of

western corn rootworm in Annual meeting of ESA Austin TX (November 10-13)

632 Shrestha R B M N Parajulee and S C Carroll 2013 Characterization of boll damage

potential of Lygus hesperus and its relevance in pest management in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

633 Shrestha R B M N Parajulee and S C Carroll 2013 Challenges and opportunities in

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 20: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 20

January 26 2014

20

thrips management in Texas High Plains cotton in 61st annual meeting of the

Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

634 Shrestha R B M N Parajulee and S C Carroll 2010 Genetic population structure of

Lygus hesperus in the Texas High Plains Beltwide Cotton Conference New Orleans LA

(Jan 4-7)

635 Shrestha R B M J D San Francisco L D Densmore R E Strauss A S Holaday

M D Burrow and M N Parajulee 2010 Determining population genetic structure of

Lygus hesperus from Texas High Plains using microsatellite markers Texas Tech Annual

Biological Sciences Symposium (TTABSS) Lubbock TX (Feb 5-7)

636 Shrestha R B A Bastola and M N Parajulee 2010 Seasonal dynamics of insect

community structure in Texas High Plains cotton Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

637 Parajulee M N S C Carroll R B Shrestha R J Kesey D M Nesmith and J P

Bordovsky 2010 Effect of nitrogen fertility on agronomic parameters and arthropod

activity in drip irrigated cotton Beltwide Cotton Conference New Orleans LA (Jan 4-7)

638 Shrestha R B M N Parajulee S C Carroll and A Bastola 2009 Evaluation of

intercrop movement of Lygus between cotton and roadside alfalfa Beltwide Cotton

Conference San Antonio TX (Jan 5-8)

639 Shrestha R B M N Parajulee and S C Carroll 2009 Challenges in tracking intercrop

movement of Lygus hesperus between cotton and alfalfa 57th

annual meeting of SWB of

ESA Stillwater OK (Feb 23-26)

6310 Shrestha R B M N Parajulee and S C Carroll 2009 Characterization of the host

selection behavior of western tarnished plant bug (Lygus hesperus) 57th

annual meeting of

ESA Indianapolis IN (Dec 13-16)

6311 Bastola A M N Parajulee and R B Shrestha 2009 Evaluation of different methods

of nuclear DNA extraction for Lygus hesperus samples preserved in various storage

conditions Beltwide Cotton Conference San Antonio TX (Jan 5-8)

6312 Adhikari M B M N Parajulee and R B Shrestha 2009 Cotton boll susceptibility to

Lygus hesperus in relation to boll age Beltwide Cotton Conference San Antonio TX

(Jan 5-8)

6313 Shrestha R B M N Parajulee M B Adhikari and R E Strauss 2008 Comparative

ontogenic morphometry Lygus hesperus vs Lygus lineolaris Beltwide Cotton

Conference Nashville TN (Jan 8-11)

6314 Shrestha R B M N Parajulee and S C Carroll 2008 Optimization of protein

marking and detection system for the study of intercrop movement behavior of Lygus

hesperus (Knight) 56th

annual meeting of ESA Reno NV (Nov 16-19)

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 21: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 21

January 26 2014

21

6315 Parajulee M N A K Barman A Balachandran C Chen R B Shrestha and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in drip-irrigated cotton Beltwide

Cotton Conference Nashville TN (Jan 8-11)

6316 Adhikari M B M N Parajulee and R B Shrestha 2008 Relationship between cotton

boll age and Lygus injury SWB of ESA annual meeting Fort Worth TX (Feb 23-26)

6317 Shrestha R B M N Parajulee and O P Perera 2007 Genetic diversity of Lygus

hesperus from different locations in Texas 55th

annual meeting of ESA San Diego CA

(Dec 9-12)

6318 Shrestha R B and M N Parajulee 2007 Morphometric analysis of Lygus species from

Texas Beltwide Cotton Conference New Orleans LA (Jan 9-12)

6319 Barman A K M N Parajulee and R B Shrestha 2007 Evaluation of compensatory

ability of cotton to fruit loss by Lygus hesperus in the Texas High Plains Beltwide Cotton

Conference New Orleans LA (Jan 9-12)

6320 Shrestha R B M N Parajulee and O P Perera 2006 Development and

characterization of SSR and ISSR markers for Lygus hesperus from Texas 54th

annual

meeting of ESA Indianapolis IN (Dec 10-13)

6321 Shrestha R B and M N Parajulee 2006 Optimization of ELISA procedure for Lygus

marking and its application in Lygus movement study 54th

annual meeting of SWB of

ESA Austin TX (Feb 27-Mar 1)

6322 Barman A K M N Parajulee S C Carroll and R B Shrestha 2006 Evaluation of

host preference of Lygus hesperus Beltwide Cotton Conference San Antonio TX (Jan 3-

6)

6323 Shrestha R B and M N Parajulee 2005 Effect of tillage system and planting date on

seasonal abundance of predacious ground beetles in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6324 Parajulee M N R B Shrestha S C Carroll K F Bronson P L Bommireddy and A

M Cranmer 2005 Variable rate of irrigation water and nitrogen in cotton Potential of

site-specific management of cotton aphids Beltwide Cotton Conference New Orleans

LA (Jan4-7)

6325 Barman A K M N Parajulee and R B Shrestha 2005 Influence of planting date and

cultivar on Lygus and cotton fleahopper activity in cotton Beltwide Cotton Conference

New Orleans LA (Jan 4-7)

6326 Shrestha R B M N Parajulee and C Blanco 2004 Predation efficiency of selected

cotton arthropod predators of bollworm eggs 52nd

annual meeting of SWB of ESA

Lubbock TX (Feb 23-26)

6327 Shrestha R B M N Parajulee and C Blanco 2004 Functional response of selected

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 22: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 22

January 26 2014

22

cotton arthropod predators to bollworm eggs in the laboratory Beltwide Cotton

Conference San Antonio TX (Jan 5-9)

6328 Shrestha R B and M N Parajulee 2004 Influence of temporal abundance and spatial

patterns of thrips and cotton fleahoppers on effectiveness of sampling methods in Texas

High Plains cotton 52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

6329 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Lygus abundance on wild hosts A survey across the Texas High

Plains Beltwide Cotton Conference Nashville TN (Jan 6-10)

6330 Parajulee M N and R B Shrestha 2003 Rate of consumption of cotton aphids by

convergent lady beetles in the laboratory Beltwide Cotton Conference Nashville TN

(Jan 6-10)

6331 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha S C

Carroll and M D Arnold 2003 Influence of cotton cultivar and planting date on

fleahopper and Lygus populations in the Texas High Plains Beltwide Cotton Conference

Nashville TN (Jan 6-10)

6332 Bommireddy P L M N Parajulee D O Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Effect of irrigation regimes on insect populations in

Texas High Plains cotton Beltwide Cotton Conference Nashville TN (Jan 6-10)

6333 Shrestha R B G Dahal R B Thapa and N K Khatri 1994 Study on leafhopper and

planthopper vectors of rice virus diseases in Nepal A bibliographic review National

Science and Technology Conference Royal Nepal Academy of Science and Technology

Lalitpur Nepal (June 8-11)

7 Poster Presentations (44 Total)

71 International Meetings (8 Posters)

711 Carroll S C R B Shrestha W O McSpadden and Megha N Parajulee 2012

Overview of Lygus Research and Outreach Program in the Texas High Plains Serving the

Clientele of the Worldrsquos Largest Cotton Patch 3rd

International Lygus Symposium

Scottsdale Arizona (28-31 October)

712 Shrestha R B and M N Parajulee 2012 IPM options for Lygus bug management in

Texas High Plains cotton 7th

International IPM Symposium Memphis TN March 27-29

2012

713 Carroll S C M N Parajulee M B Adhikari and R B Shrestha 2010 Lygus hesperus

injury to maturing bolls Effects on quality and quantity of cotton yield Joint meeting of

Mexican Entomological Society and 58th

annual meeting of SWB of ESA Cancun

Mexico (Apr 11-14)

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 23: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 23

January 26 2014

23

714 McSpadden W O M N Parajulee S C Carroll and R B Shrestha 2010 Effect of

landscape level habitat heterogeneity on the abundance of Lygus hesperus in cotton Joint

meeting of Mexican Entomological Society and 58th

annual meeting of SWB of ESA

Cancun Mexico (Apr 11-14)

715 Parajulee M N S C Carroll and R B Shrestha 2010 Cotton entomology research

and outreach in the Texas High Plains A historic journey of the Texas AampM Lubbock

Center Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB

of ESA Cancun Mexico (Apr 11-14)

716 Shrestha R B and M N Parajulee 2010 Optimization of indirect ELISA protocol for

the detection of marker protein on Lygus hesperus Joint meeting of Mexican

Entomological Society and 58th

annual meeting of SWB of ESA Cancun Mexico (Apr

11-14)

717 Bastola A M N Parajulee and R B Shrestha 2010 Population dynamics and

intercrop movement behavior of convergent lady beetle in cotton-alfalfa agroecosystem

Joint meeting of Mexican Entomological Society and 58th

annual meeting of SWB of

ESA Cancun Mexico (Apr 11-14)

718 Parajulee M N and R B Shrestha 2004 Potential of convergent lady beetle

Hippodamia convergens in suppressing cotton aphids Aphis gossypii 15th

International

Plant Protection Congress Beijing China (May 10-16)

82 Posters in National Meetings (36)

821 Shrestha R B and M N Parajulee 2013 Cotton plant growth and yield response to

various levels of Lygus infestations in 61st annual meeting of Entomological Society of

America Austin TX (November 10-13)

822 Vandiver M M N Parajulee D Wann R B Shrestha J Dever M Arnold A K

Barman 2013 Potential of Entrustreg SC insecticide on western flower thrips management

in organic cotton in 61st annual meeting of Entomological Society of America Austin

TX (November 10-13)

823 Carroll S R B Shrestha and M N Parajulee 2013 A decade of Lygus research in

Texas High Plains Research highlights and clientele outreach in 61st annual meeting of

the Southwestern Branch of the Entomological Society of America Las Cruces NM

(February 25-28)

824 Parajulee M N R B Shrestha M R Vandiver D Q Wann J K Dever and M D

Arnold 2013 Evaluation of organic pesticides for western flower thrips management in

seedling cotton Effect on plant parameters in Beltwide Cotton Conferences San Antonio

TX (January 7-10)

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 24: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 24

January 26 2014

24

825 Vandiver M R R B Shrestha J K Dever and M D Arnold And M N Parajulee

2013 Managing thrips using organically approved insecticides in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

826 Brewer M J M N Parajulee D J Anderson and R B Shrestha 2013 Plant water

stress and insect seasonality effects on Cotton Fleahopper damage in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

827 Carroll S C R B Shrestha and M N Parajulee 2013 Effect of nitrogen fertilizer on

cotton host-plant quality and its impact on arthropod community in Beltwide Cotton

Conferences San Antonio TX (January 7-10)

828 Carroll S C R B Shrestha W O McSpadden A Bastola and M N Parajulee 2012

Growth and developmental response of cotton cultivars to different levels of Lygus

infestation in Beltwide Cotton Conferences Orlando FL (January 3-6)

829 Parajulee M N R B Shrestha W O McSpadden S C Carroll Y Yang and L T

Wilson 2012 Quantification of cotton plant growth response to cotton fleahopper

infestations in Beltwide Cotton Conferences Orlando FL (January 3-6)

8210 Shrestha R B W O McSpadden J K Dever M D Arnold D L Kerns and M N

Parajulee 2012 Potential of organic pesticides for western flower thrips management in

seedling cotton effect on plant parameters in Beltwide Cotton Conferences Orlando FL

(January 3-6)

8211 Brewer M J M N Parajulee D J Anderson R B Shrestha 2012 Cotton Fleahopper

and its damage to cotton as affected by plant water stress and insect seasonality in

Beltwide Cotton Conferences Orlando FL (January 3-6)

8212 Shrestha R B S C Carroll and M N Parajulee 2010 Effect of roadside alfalfa

mowing on the abundance of Lygus hesperus in adjacent cotton field A field marking

study Beltwide Cotton Conference New Orleans LA (Jan 4-7)

8213 Shrestha R B M N Parajulee and S C Carroll 2010 Evaluation of molecular markers

for genetic diversity studies of western tarnished plant bug (Lygus hesperus) 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8214 Carroll S C M N Parajulee W O McSpadden and R B Shrestha 2010 Influence of

proximity and abundance of non-cotton hosts on Lygus hesperus in cotton 58th

annual

meeting of ESA San Diego CA (Dec 12-15)

8215 Adhikari M B M N Parajulee R B Shrestha and D L Kerns 2010 Lygus hesperus

feeding on maturing cotton bolls Adults vs nymphs Beltwide Cotton Conference New

Orleans LA (Jan 4-7)

8216 Parajulee M N A K Barman R B Shrestha and S C Carroll 2009 Plant

compensation of Lygus-induced fruit loss in cotton How much can we tolerate 57th

annual meeting of ESA Indianapolis IN (Dec 13-16)

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 25: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 25

January 26 2014

25

8217 Parajulee M N A K Barman R B Shrestha C Chen A Balachandran and S C

Carroll 2008 Compensation of Lygus-induced fruit loss in Cotton 56th

annual meeting of

SWB of ESA Fort Worth TX (Feb 23-26)

8218 Shrestha R B and M N Parajulee 2007 Morphometric comparison of Lygus species

55th

annual meeting of SWB of ESA Corpus Christi TX (Feb 19-22)

8219 Parajulee M N A K Barman R B Shrestha C Chen A K Balachandran and S C

Carroll 2007 Compensation of Lygus-induced fruit loss in cotton 55th

annual meeting of

ESA San Diego CA (Dec 9-12)

8220 Shrestha R B M N Parajulee and O P Perera 2006 Construction of microsatellite-

enriched genomic DNA library of Lygus hesperus 54th

annual meeting of SWB of ESA

Austin TX (Feb 27-Mar 1)

8221 Albeldano W A J Slosser M N Parajulee D G Bordovsky R B Shrestha and J

W Sij 2006 Influence of irrigation method and tillage system on cotton fleahopper

activity Beltwide Cotton Conference San Antonio TX (Jan 3-6)

8222 Parajulee M N R B Shrestha James F Leser 2004 Effect of tillage planting date

and cultivar on western flower thrips seasonal dynamics patterns in cotton 52nd

annual

meeting of ESA Salt Lake City UT (Nov 14-17)

8223 Cranmer A M M N Parajulee J F Leser R B Shrestha and P L Bommireddy

2004 Use of COTMAN expert system software in cotton insect management research

52nd

annual meeting of SWB of ESA Lubbock TX (Feb 23-26)

8224 Cranmer A M M N Parajulee J F Leser P L Bommireddy and R B Shrestha

2004 Use of COTMAN in insect management research Beltwide Cotton Conference San

Antonio TX (Jan 5-9)

8225 Shrestha R B M N Parajulee and A M Cranmer 2003 Influence of planting date

and cultivar on cotton arthropod predators 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8226 Shrestha R B M N Parajulee A M Cranmer P L Bommireddy S C Carroll and

M D Arnold 2003 Influence of conservation tillage system on cotton arthropod

predators 51st annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

8227 Shrestha R B and M N Parajulee 2003 Temporal and spatial patterns of cotton

arthropod predators in conservation tillage system 51th

annual meeting of ESA

Cincinnati OH (Oct 23-29)

8228 Parajulee M N M D Arnold S C Carroll A M Cranmer R B Shrestha and P L

Bommireddy 2003 Non-cotton host source of Lygus bugs in the Texas High Plains 51st

annual meeting of SWB of ESA Oklahoma City OK (Feb 22-27)

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 26: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 26

January 26 2014

26

8229 Parajulee M N M D Arnold S C Carroll A M Cranmer P L Bommireddy and R

B Shrestha 2003 Lygus bugs in Texas cotton Significance of roadside non-cotton hosts

51th

annual meeting of ESA Cincinnati OH (Oct 23-29)

8230 Cranmer A M M N Parajulee J F Leser P L Bommireddy R B Shrestha SC

Carroll and M D Arnold 2003 Seasonal activity patterns of Lygus species as affected by

planting date and cotton cultivar 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8231 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Influence of

pigweed on beet armyworm activity in cotton 51st annual meeting of SWB of ESA

Oklahoma City OK (Feb 22-27)

8232 Carroll S C M N Parajulee R B Shrestha and M D Arnold 2003 Beet armyworm

population abundance in cotton as affected by pigweed Beltwide Cotton Conference

Nashville TN (Jan 6-10)

8233 Bommireddy P L M N Parajulee DO Porter R B Shrestha A M Cranmer M D

Arnold and S C Carroll 2003 Influence of irrigation regime on seasonal abundance

patterns of Lygus species in cotton 51st annual meeting of SWB of ESA Oklahoma City

OK (Feb 22-27)

8234 Shrestha R B M N Parajulee S C Carroll and A M Cranmer 2002 Arthropod

predators in conventional and conservation tillage systems in cotton 50th

annual meeting

of ESA Fort Lauderdale FL (Nov14-20)

8235 Parajulee M N A M Cranmer P L Bommireddy R B Shrestha S C Carroll and

M D Arnold 2002 Host plant sequence of Lygus bugs in Texas cotton

50th

annual meeting of ESA Fort Lauderdale FL (Nov14-20)

8236 Cranmer A M M N Parajulee P L Bommireddy M D Arnold and R B Shrestha

2002 Cotton cultivars and planting date window Do Lygus bugs care 50th

annual

meeting of ESA Fort Lauderdale FL (Nov14-20)

Other Meetings Attended (4 Total)

2003 West Texas Agricultural Chemicals Institute Conference Lubbock TX participated as a

WTACI scholarship award winner

1998 Fourth International Conference of Asian Apiculture Association International Center for

Integrated Mountain Development Lalitpur Nepal participated as DAO representative

(March 23-27)

1993 National Conference on Biotechnology Biotechnology Association of Nepal Kirtipur

Nepal participated as a student volunteer (Apr 29-30)

1993 First Regional Conference on Prospect of Biotechnology in Nepal Biotechnology and

Biodiversity Society of Nepal Birgunj Nepal participated as an organizing member (Apr

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 27: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 27

January 26 2014

27

11-13)

Professional Trainings and Workshops (14 total)

2014 Registered for 2-day bioinformatics workshop ldquoIowa State software carpentry

bootcamprdquo at ISU Ames IA (January 8 and 9)

2013 Participated 1-day ldquoGrant writing Seminar and workshoprdquo at Memorial Union ISU

Ames IA (October 3)

2010 Completed training on ldquoBlackboard online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 COTMAN Plant Mapping Workshop Texas AgriLife Research Lubbock TX (June 30)

2009 Completed training on ldquoWebCT online teaching course preparationrdquo at TLTC TTU

Lubbock TX

2009 Completed training on ldquoRadioactive material handling and laboratory safetyrdquo at Texas

AgriLife Research Lubbock TX (June 30)

2009 Photoshop SAS and Matlab training at TLTC TTU Lubbock TX

2006 Completed training on ldquoShipping and receiving hazardous materials Information

security awareness Ethics in the workplace and Creating a discrimination-free

workplacerdquo sponsored by Texas AampM University

2005 Attended a 2-day training session on ldquoMolecular Techniques for Arthropod Monitoringrdquo

at Dr James Haglerrsquos Laboratory USDA-ARS Western Cotton Research Laboratory

Phoenix AZ Learned marking techniques for Lygus bugs using different proteins and

detection of marked Lygus by direct and DAS-ELISA technique (March 17-18)

2005 Training on genomic DNA library construction and microsatellite marker development

and genotyping in SIMRU USDA Stoneville MS

2004 Participated in a 1-day Grant Writersrsquo Seminar and Workshop ldquoWrite Winning Grantsrdquo

Office of Research Services Texas Tech University (Apr 22)

2001 Netherlands Fellowship Programmes international course on IPM in IAC Wageningen

the Netherlands (March 18 - June 30)

2000 UNDP-IPM international course on crop protection in CICAT Guangzhou China (May

5 - July 4)

1998 Rice IPM trainerrsquos training Nepal (Apr 6 - July 10)

1993 Diploma in Biotechnology Nepal (Feb 13 - May 13)

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 28: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 28

January 26 2014

28

Other Outreach Activities

2011 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2011rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 23-26)

2009 Displayed a 4-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2009rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (8000 people in attendance) This

effort also involved eight graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 18-21)

2008 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2008rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (6000 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 27-29)

2007 Displayed a 3-day ldquoInsect Boothrdquo at the Lubbock Science Spectrum ldquoCritter fest 2007rdquo

and presented live demonstrations and lectures on insect feeding growth and

development and insect management in field crops (5700 people in attendance) This

effort also involved six graduate students to assist with this entomology-awareness

public outreach program Lubbock TX (June 15-17)

Research Experience

I have gained thorough understanding and experience in independently designing developing

and conducting scientific experiments from concept development proposal writing and

experimental design to observation and data collection analysis interpretation of results and

eventual publication and presentation I have spent significant time (gt10 years) executing

entomological research projects as a team leader collaborator or assistant and have authored or

co-authored about 100 publications and presentations based upon results obtained

Insect species studied Phytophagous Hemipterans (Lygus hesperus [western tarnished plant

bug] and Pseudomatoscelis seriatus [cotton fleahopper]) Homoptera (rice green leaf hopper

and plant hopper) Lepidoptera (cotton bollworm beet armyworm tobacco budworm)

Thysanoptera (western flower thrips) Coleoptera (Western Corn Rootworm [Diabrotica

virgifera] predacious ground beetles convergent lady beetles) numerous other insect pest

and predator species found in corn cotton and alfalfa during survey activities Studies

included community structure and species diversity

Field crop experience Corn and cotton rice alfalfa papaya peanut soybean (familiar with

agronomy and pest management of many crop species of legumes vegetables fruits small

grains and oilseeds crops during my time in Nepal)

ScaleScope of experiments conducted

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 29: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 29

January 26 2014

29

I have conducted various level of research from subcellular level (molecular biology study) to

agro-ecosystem and landscape level studies I have conducted studies in growth chambers

(biology physiology morphology and behavioral bioassay) green house field cages (clip-

cage cup-cage whole plant cage and large field cages) large field plot and landscape level

studies

Types of experiments conducted and skilled developed (see page 16-35 for detail publication

listing for all categories of research)

Categories Research Projects Skill amp Experience Gained

Insect mass

rearing Western Corn Rootworm Lygus

cotton fleahopper aphid thrips

lady beetles bollworms and

green leaf hoppers mass rearing

in laboratory

Colony care from egg to adult

Artificial diet preparation colony

health management

Developmental

biology Ontogenic morphometry of Lygus

species

Growth and developmental

biology of bollworm

Morphometry- monitoring

growth and development shape

and size change and ontogenic

trajectories

Feeding

biology Effect of elevated CO2 on feeding

of cotton bollworm

Effect of temperature on

bollworm egg consumption by

various predators

Functional response of lady

beetles feeding on cotton aphids

Effect of cotton boll maturity on

Lygus feeding injury

Functional response bioassay

whole-plant cage study

cup-cage study two choice and

no choice bioassay

Vector biology Rice tungro virus transmission

using green leaf hoppers

Test tube and mass inoculation

transmission assays

Foraging

behavior Host selection behavior of Lygus

Boll selection behavior of Lygus

Intercrop movement behavior

Protein marking and movement

detection

Host preference bioassay Y-tube

bioassay

Insect

physiology +

pesticide

resistance

Metabolism cold tolerance

overwintering diapause spring

emergence and pesticide

resistance of Lygus

Cold bath study emergence cage

dig-up cage study and glass vial

pesticide bioassay

Molecular

biology DNA extraction method

optimization

Molecular marker development

Molecular marker comparison

Protein detection

QTL analysis

DNA extraction genomic DNA

library development

microsatellite marker discovery

AFLP ISSR RAPD SSR

genotyping QTL analysis

ELISA

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 30: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 30

January 26 2014

30

Population

genetics Microsatellite marker based

population structure and genetic

diversity study of Lygus hesperus

SSR based genotyping by

capillary electrophoresis

Systematics

phylogenetic Mitochondrial gene sequence

based phylogeny

Various gene sequencing and

DNA sequence analysis

Insect control-

IPM Field experiments on cultural

control of cotton insect pests

(cultivar tillage planting date

soil nitrogen application

irrigation and weed host

management)

Field plot techniques cotton

agronomy-planting irrigation

fertility management weed

management fruiting and

flowering profile monitoring

harvesting ginning

Sampling and

population

modeling

Sampling plan development and

sampling method comparison

Mechanistic modeling of aphid

population

Sampling plan development

population modeling

Bt-transgenic

trait

technology

evaluation

Performance of Bt-cotton in

different tillage systems different

level of CO2 concentration

Effect of Bt-cotton on target and

non-target insect pests

Screening of transgenic events

Conservation tillage

OTC microcosm experiments

Monsantorsquos regulated field trials

Plant tissue bioassay

Field cage study

Bt-resistance

evolution and

resistance

management

(IRM)

WCR Field resistance monitoring

and detection

Inheritance stability and fitness

cost of resistance trait

Characterizing cross resistance

Development of IRM plan

Single plant seedling mat and

diet bioassay technique

Laboratory rearing hybridization

and fitness parameter

measurement

Bt-trait evaluation through field

plot experimentation

Insect-plant

interactions Crop compensation and plant

growth response to fleahopper

Cotton varietal response

Field trial cotton growth

response measurement

Landscape

ecology Effect of habitat heterogeneity

and spatial characteristics

Habitat mapping insect survey

spatial analysis

Biological

control Pest population suppression and

prey consumption rate

Large cage field study and prey

feeding bioassay

Insect ecology Survey of Lygus abundance

Survey of rice green leaf hoppers

Survey sampling

Community

ecology Species diversity of predacious

ground beetles

Species diversity of arthropods

Insect collection preservation

identification community

structure in cotton and alfalfa

Literature

review Bibliographic database

development and literature

review

Collection arrangement review

and analysis

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 31: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 31

January 26 2014

31

Summary of Research Accomplishments and Work History

Project1 Rice virus disease and its green leaf hopper vector (1992-1994)

I conducted an extensive survey of rice tungro virus disease and its leaf hopper vectors in Nepal I

screened the 15 rice cultivars for of tungro virus susceptibility and also identified 12 alternate

weed hosts of tungro virus Biological and molecular characterization of the Nepalese strain of

tungro virus was performed in laboratory and green house We developed the restriction map of

DNA from RTBV In addition we developed the bibliographic database of tree and plant

pathogen in Nepal and I published a comprehensive review of literature on plant pathology and

rice plant and leaf hopper vectors

Project2 Evaluation of Bt-transgenic technology in elevated CO2 and different crop

management conditions (2002-2004)

I investigated how Bt-cotton can influence on the population of non-target insect pest species such

as thrips cotton fleahopper cotton aphid and Lygus bug I was first to characterize the season-

long population dynamics of thrips and cotton fleahopper in Bt and non Bt cotton in both

conventional and conservation tillage system in Texas high Plains In collaboration with Chinese

Academy of Science we also evaluated the effect of elevated CO2 on cotton plant physisology

and boll worm biology We discovered that elevated CO2 lowers the level of Bt-toxin production

in Bt-transgenic cotton plant and it also reduces the body mass fecundity and growth rate of

cotton bollworm In collaboration with Monsanto we evaluated bollgard technology for cotton

bollworm management and from 2010 I am also involved in evaluation of transgenic cotton for

Lygus management

Project3 Insect community ecology (20022009)

In 2002 I characterized the seasonal dynamics of ground beetle species diversity in Texas High

Plains cotton field and discovered that the Texas High Plains agro-ecosystem had very low

ground beetle diversity I demonstrated that predacious ground beetle diversity can be increased

significantly by adoption of conservation tillage system We also characterized the insect

community structure in furrow irrigated cotton field adjacent to alfalfa in 2009

Project4 Role of predators in biological control of bollworm egg and cotton aphids (2003-

2004)

I was the first researcher to characterize the functional response (density dependent predation)

behavior of eight predator species feeding on bollworm eggs in a single comprehensive laboratory

experiment I also quantified the cotton aphid suppression potential of convergent lady beetle both

in field and laboratory settings I demonstrated that convergent lady beetles have potential to

suppress larger populations of cotton aphids and cotton bollworms I also determined the species

composition of arthropod predator community in a cotton field in the Texas High Plains

Project5 Cotton insect sampling plan and predictive mechanistic model development

(2002-2004)

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 32: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 32

January 26 2014

32

I developed fixed precision sequential sampling plan for thrips and cotton fleahopper population

determination in cotton using visual sampling I evaluated various sampling techniques for thrips

and cotton fleahopper to determine the most efficient sampling technique in cotton We also

developed the mechanistic model for predicting cotton aphid population dynamics which

effectively determines five response variables including birth rate death rate cumulative density

population peak count and time of peak count I determined that irrigation water levels can

significantly affect cotton aphid population dynamics in low precipitation (lt25 cm) years

Project6 Insect marking and movement behavior study and landscape level factors

predisposing Lygus outbreak (2005-2010)

I conducted extensive survey of weed host of Lygus in Texas High Plains and identified 23

reproductively suitable weed hosts of Lygus I also determined the Lygus species composition in

different hosts I optimized the methods for protein marking of natural Lygus population in cotton

and alfalfa field I characterized the seasonal dynamics of intercrop movement behavior of Lygus

in cotton and alfalfa cropping system in Texas using field marking and ELISA techniques I also

conducted the landscape level study of Lygus ecology and developed a predictive model to

describe Lygus abundance in cotton field using the surrounding landscape characteristics affecting

Lygus populations in cotton

Project7 Insect plant interactions (crop compensation boll maturity threshold study etc)

(2007-2011)

We have developed the insect-plant interaction based IPM rules for initiation and termination of

pesticide intervention for Lygus in Texas High Plain cotton I demonstrated that cotton plant can

easily compensates about 37 square loss due to early season Lygus infestation in high input

farming system (drip irrigation) in the Texas High Plains environment thus the early pesticide

application is not justified if fruit loss is below 37 We also characterized the cotton plantrsquos lint

yield compensation capacity for different cotton cultivars different phenological stages and

different irrigation water levels I determined the cotton boll damage potential of Lygus adults and

nymphs at various levels of cotton boll maturity I determined that once cotton boll reaches 350

cumulative heat unit or 25 mm in boll diameter they are considered safe from internal boll

damage by Lygus Because there will be no yield loss when Lygus bugs are unable to penetrate

the internal carpel wall pesticide application against Lygus is not justified beyond 350 heat unit

or when bolls attain 25 mm diameter in size

Project8 Molecular ecology marker development and population genetics of Lygus (2008-

2009)

I was the first person to develop microsatellite enriched genomic DNA library of Lygus hesperus

After sequencing the DNA library I discovered and characterized polymorphic microsatellite

markers Using those microsatellite markers I have determined the genetic diversity and

population genetic structure of Lygus hesperus from the Texas High Plains I have also optimized

the methodology for the genomic DNA extraction from gt5 years old dry preserved Lygus

hesperus samples

Project9 Lygus ontogenic morphometry (2010)

I was the first scientist to conduct the detailed ontogenic morphometry of Lygus hesperus I

demonstrated that ontogenic morphometry of live Lygus hesperus is possible without killing the

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 33: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 33

January 26 2014

33

organism for its measurement I have developed the ontogenic growth model for male and female

Lygus hesperus that allowed us to predict both shape and size of this species at any particular age

I have demonstrated that landmark based morphometric technique is the most appropriate

morphometric approach in quantifying the growth and development of Lygus species I have also

discovered that multivariate analysis of four inter landmark distances can clearly distinguish 5

different nymphal stages of Lygus hesperus with high accuracy In addition I presented detailed

ultrastructure morphology of Lygus hesperus using scanning electron microscopy

Project10 Ecologically intensive integrated pest management (Evaluation of tillage

planting date skip row planting pattern irrigation cultivar and soil nitrogen (2000-2012)

I have developed cultural management strategies for different cotton pests I evaluated the

possibility of insect pest population suppression by various cultural practices like conservation

tillage different cotton cultivars changing cotton planting date change in amount of irrigation

changing method of irrigation method changing cotton planting pattern and plant density and

various level of nitrogen application We conducted series of field experiments over many years

to evaluate the possibility of population suppression of thrips cotton fleahopper cotton aphid

Lygus and bollworm using aforementioned cultural practices

Project11 Characterization of field evolved Bt-resistance in Western Corn Rootworm

(Diabrotica virgifera virgifera) and IRM

httpwwwentiastateedudeptfacultygassmannpeopleram-shrestha

My current research projects are focused on characterization of field evolved Bt-resistance in

Western Corn Rootworm (Diabrotica virgifera virgifera) I am involved in monitoring and

quantifying evolution of Bt-resistance and cross resistance in WCR field populations from various

locations in Iowa for four Bt proteins (Cry3Bb mCry3A eCry31A and Cry3435Ab) We use

single plant bioassay seedling mat bioassay diet bioassay and field experiments to determine the

Bt-resistance I evaluated performance of commercially available corn hybrids with single gene

or stacked gene Bt-traits in controlling WCR in corn field We are developing dynamic economic

threshold for WCR management in Iowa I am also involved in investigation of inheritance

stability and fitness cost of the Bt- resistance of WCR in laboratory We are conducting QTL

analysis of Bt-resistance using SNP markers and planned to develop molecular marker for Bt-

resistant trait

I have a decade of research experience and interest in insect ecology and biology More

specifically insect-plant interaction intercrop movement integrated pest management insect

morphometry landscape management population genetics genetic diversity study molecular

marker development Bt-resistance management and sampling plan development for various pest

species

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom

Page 34: Curriculum Vitae - Entomology › files › people › cv › RB... · Organized and moderated symposium in national and regional levels entomology society meetings Taught 3 graduate

RB Shrestha Page 34

January 26 2014

34

References

1 Dr Aaron Gassmann Assistant Professor Department of Entomology Iowa State

University Ames IA Phone 515-294-7623 E-mail aaronjgmailiastateedu

2 Dr Megha Parajulee Professor and Regents Fellow Texas AampM AgriLife Research and

Extension Center Lubbock TX Phone 806-746-6101 E-mail mparajulagtamuedu

3 Dr Eduardo Segarra Chair and Professor Department of Agricultural and Applied

Economics Texas Tech University Phone 806-742-2821 E-mail eduardosegarrattuedu

4 Dr Chris Sansone Global Regulatory Affairs Manager Bayer CropScience LP

BioScienceIRM (Americas Region) 2 TW Alexander Drive Research Triangle Park NC

27709-2014 Phone +1 919 549 2880 Mobile +1 919 450 7903 Fax +1 919 549 2892

E-mail chrissansonebayercom