Cumulative subject index volumes 92–100

ANALYTICAL BIOCHEMISTRY 100, 376-430 (1979)

CUMULATIVE SUBJECT INDEX’

Volumes 92-100

A

Abscisic acid chromatographic analysis (potato), 97, 33 1

Absorbance aminco-chance dual wavelength split-beam record-

ing spectrophotometer, use limitations and interpretation, 94, 450

red-shift in absorbance maximum of ethidium on reaction with tetraphenylboron, 92, 462

Absorption atomic

digestion of muscle biopsies for trace metal analysis using organic tissue solubilizer (human), 98, 315

atomic spectrometry, application to trace metal analysis

in silkworm eggs and hatched worms, 94, 386

molar coefficient, 2-nitrobenzoic acid (Ellman’s reagent), 94, 75, erratum, 99, 23 1

spectrum assay for measurement of urinary p-aminobenzoic

acid in oral pancreatic function test (human), 98, 13

estimation of glycosaminoglycan concentrations, 99, 183

N-(2-Acetamidohminodiacetic acid free metal ion depletion by “Good’s” buffers,

95, 527 Acetanilide

separation and chromatographic determination of acetanilide 4-hydroxylase activity (mouse), 96, 201

Acetanilide 4-hydroxylase activity, chromatographic quantitative determina-

tion (mouse), 96, 201 Acetoacetate

determination of radioactivity, 92, 3% Acetoacetyl coenzyme A

spectrophotometric cycling assay of tissue levels, 97, 103

Acetone determination of radioactivity, 92, 390 radioactive, reagent in radiometric determination

of L-canaline, 92, 265 Acetonitrile

chromatographic separation of acetylated oligosaccharides, 97, 184

Acetyl coenzyme A spectrophotometric cycling assay of tissue levels,

97, 103 Acetyl coenzyme A hydrolase

charcoal adsorption for microassay, 93, 244 Acetylation

amino sugar methyl glycosides, 94, 36 discriminating galactosamine and glucosamine in

mixtures, 94, 394 Acetylcholine hydrolase

assayed, nature of random experimental error, 94, 265

N-Acetyl-o-ghrcosamine binding lectins, model system for study of binding

specificity, 99, 221 N-Acetyl-P-o-glucosaminidase

DNA, protein, and enzyme activity, fluorometric assay (rat), 94, 166

N-Acetyl-L-glutamic acid y-semialdehyde enzymic synthesis, isolation and characterization

of 2,4-dinitrophenylhydrazones, 96, 94 Acid ethanol

extracted lymphoid interferons, purification by Blue Sepharose chromatography (human), 98, 214

Acids, see also Amino acids and speciJic acids

cinnamic, reverse-phase high-performance liquid chromatographic separation, 93, 233

hexuronic, quantitation by modified carbazole method, 93, 295

4’-(9-Acridinylamino)methanesulfon-m-anisidide antitumor agent, fluorescence assay based on alka-

line hydrolysis, 96, 504 Acriflavine

fluorescent labeling of high molecular weight RNA, 93, 399

Actins plasma membrane, isoelectric focusing (chick fibro-

blasts), 95, 236 solid-phase assay using protein-A (mouse), 94, 140 thin-layer peptide map (chicken, rabbit), 94, 150 viscosity, interaction with chaetoglobosins or cyto-

chalasins (rabbit muscle), 95, 14 Acycloguanosine

radioimmunoassay for antiviral agent BW248U [9-(2-hydroxyethoxymethyl)guanine], 98, 319

’ Boldface numbers indicate appropriate volume; lightface numbers indicate pagination.

376 Copyright 0 1979 by Academic Press, Inc. All rights of reproduction in any form reserved.

Acyl coenzyme A analysis and purification of thioesters, by reversed-

phase ion-pair liquid chromatography, 94, 417 long-chain derivatives, chromatographic determina-

tion of CoASH, %, 181 Acyl-coenzyme A synthetase

activation of serum free fatty acids, enzymatic microdetermination (human, rabbit), 98, 341

Acylcamitines

glass beads, isolation and purification of type I procollagen (human), 96, 433

Affinity chromatography albumin on bromosulfophthalein-glutathione con-

jugate covalently linked to Sepharose 4B (rat plasma), 92, 290

chromatographic and bioautographic method for detection utilizing mutant (Torulopsis bovina),

96, 322 Adenine

binding of fatty acids and drugs to immobilized serum albumin (bovine), 99, 352

dithiobis-(nitrobenzoic acid) induced inter- and intramolecular crosslinks in histone H3, 94, 465

folate-Sepharose column for protein fractionation, 98, 184

coupled optical assay for determination in mixtures, 98, 213

Adenosine enzymic conversion to hypoxanthine, fluorimetric

assay, 95, 377

high-capacity lectin column, glycoprotein purification (wheat germ), 97, 423

immobilized anhydrotrypsin as biospecific adsorbent for peptides produced by trypsin-like proteases (bovine), 98, 198

Adenosine deaminase coupled optical assay for determination of adenine

in mixtures, 98, 213 P1,P5-di-(Adenosine-S’)-pentaphosphate

selective inhibition of adenylate kinase activity, measurement of mitochondrial oxidative phosphorylation (rat), 96, 7

Adenosine phosphorylase coupled optical assay for determination of adenine

in mixtures, 98, 213 Adenosyl methionine

radioactive assay for guanidoacetate methyltransferase, 95, 87

interaction of 6-thiopurines and thiol-containing RNA with cellulose mercurial, 92, 324

isolation of protease inhibitor (human urine), 92, 383

lectin, isolation of an arabinogalactan protein from the style canal of Glaciolus, 92, 41

matrix, electrophoretic desorption of human serum albumin, 92, 144

porous silica gel as bioadsorbent and biocatalyst, 98, 238

purification and characterization of glycoprotein of tumor

virus (mouse), 94, 40

Adenosylhomocysteine inhibition of guanidoacetate methyltransferase, 95,

87 Adenylate kinase

activity inhibited, measurement of mitochondrial oxidative phosphorylation (rat), 96, 7

Adenylyl

aspartate transcarbamylase from germinated seedlings (Phaseolus aureus), 95,401

creatine kinase isozymes with blue Sepharose CLdB (Lepomis cyanellus), 94, 89

glutathione reductase from erythrocytes on 2’,5’- ADP-Sepharose and crystallization of enzyme (porcine), 98, 335

poly(A)-containing RNA and poIy(A) electrophoretic selection and fractionation, 92, 280

Adenylyl cyclase assay modification for guanylyl cyclase in subcellular

tissue, 93, 124 Adenylylation

glyoxalase I from human erythrocytes, 92, 390

glutamine synthetase, enzymic procedures for determining average state (Escherichiu cofi), 95,

275 Adipocytes

guanosine triphosphate cyclohydrolase I and dihydrofolate reductase, 108, 122

S-2-hydroxyacylglutathione hydrolase from erythrocytes (rat), 98, 472

malic enzyme from heart mitochondria (bovine), loo, 299

plasma membranes, isolation using diazotized diiodosulfanihc acid, 96, 236

Adrenodoxin

neurophysins on methionyl-tyrosyl-phenylalanyl- aminohexyl-agarose and measurement of ligand binding parameters, 97, 302

phosphotransacetylase (Clostridium kluyveri),

95, 2

liberation of labile sulfur from ferredoxins by alkahne zinc reagent, 95, 452

Adriamycin quantitative DNA assay (bovine), 95, 559

Adsorption

potato lectin on a N,N’,N”-triacetylchitotriose- Sepharose matrix, 93, 88

quantitative uses (review), 97, 1 synthesis of 8-(6-aminohexyl)-amino-GTP and -GDP

and application as ligands, 99, 189 Aflatoxins

charcoal, microassay of acetyl-CoA hydrolase, 93, 244

high-performance liquid chromatography analysis, 93, 409

CUMULATIVE SUBJECT INDEX 377

378 CUMULATIVE SUBJECT INDEX

Agarose electrophoretic selection and fractionation of

poly(A)-containing RNA and poly(A), 92, 280 -linked antigen, detection of immunocomplex with

antibody (mouse, rabbit), 94, 140 - methionyl - tyrosyl - phenylalanyl - aminohexyl,

chromatographic purification of neurophysins and measurement of ligand binding parameters, 97, 302

Agglutinin binding to synthetic glycoconjugates, N-acetyl-

o-glucosamine binding lectins, 99, 221 factors affecting binding of glycoprotein and the

dissociation of their complexes (Ricinus communis), 99, 254

Aggregation enzyme stabilization by immobilization (review),

93, 1 simultaneous measurement with secretion, oxygen

uptake, proton production, and intracellular metabolites in platelet suspension, 97, 387

Agmatine spectrophotometric estimation of arginine decar-

boxylase (oat seedlings), 92, 33 1 Alanine aminotransferase

assay for coupling to glutamate decarboxylase and measuring WO, release (Escherichia coli), 96, 24

Albumin affinity chromatography on bromosulfophthalein-

glutathion conjugate covalently linked to Sepha- rose 4B (rat plasma), 92, 290

bilirubin binding, fluorometric study of partition of bilirubin among blood components (human), loo, 25

binding of fatty acids and drugs, chromatographic study (bovine), 99, 352

immobilized, porous silica gel as bioadsorbent and biocatalyst (human), 98, 238

interferometric technique for determination of electrophoretic mobility of macromolecules (bovine), 96, 355

kinetics of digestion with chymotrypsin, application of protein separation by molecular weight (bovine), 98, 410

serum desorption from cibacron blue (human), 92,

144 enzyme immunoassays using urease conjugates

(bovine), 95,483 Alcian blue

quantitation and interaction of glycosaminoglycans, 93, 60

Alcohol dehydrogenase activity, photometric assay in blood serum (equine,

human), 99, 65 assayed, nature of random experimental error,

94, 265 -ethyl ester assay for serine proteases, merits of

chromophoric and fluorogenic leaving groups (human, porcine), 99, 53

hepatic, preparation of EE isozyme (equine), 99, 392

substrate, stereoselective preparation of deuterated reduced nicotinamide adenine nucleotides and substrates by enzymatic synthesis, 96, 334

Alcohol sulfotransfetase enzyme transferred 3’-phosphoadenosine-Y-phos-

phosulfate, fiber binding assay, 95, 82 Aldehyde dehydrogenase

substrate, stereoselective preparation of deuterated reduced nicotinamide adenine nucleotides and substrates by enzymatic synthesis, 96, 334

Aldehydes chromatography-spectrometry, estimation of

sphingolipids with reference to plasma ceramides (human), 97, 323

test, determination of amino groups on amino propyl glass, 94, 60

Alditol acetate consolidation of procedure modifications for estima-

tion of neutral sugars, 96, 282 Alditols

chromatographic quantitation of sugars from differential culture media, 99, 365

Aldoses chromatographic quantitation of sugars from differ-

ential culture media, 99, 365 Allophycocyanin

determination of thioether-linked cysteine residues, 92, 489

Allopurinol chromatographic simultaneous analysis with hy-

poxanthine, xanthine, oxipurinol, and uric acid, 98, 18

Aluminium hydroxide urease system, immobilization for determination of

enzyme structure, 100, 54 Amberlite XAD-2

removal of Triton X-100 from aqueous solution, 92, 447

Amidases spectrometric determination of activity, 95, 436

Amido black properties as proteins stain and dye impurities

and effect on protein-dye binding within gels, 96, 263

Amines biogenic, determination using heterocyclic cation

and aromatic anion elution, 97, 282 derivatives, interfering effect on Lowry method

protein assay (review), 180, 201 micromethod for assay with fluorescamine, re-

examination of conditions of reaction, 99, ,379 organic, chromatographic resolution of benzo[a]-

pyrene metabolites and application to marine metabolites (Stenotomus versicolor), 99, 129

Amino acid analyzer


analysis of neutral sugars as glycamines, 97, 166

spacing in isotachophoresis on polyacrylamide gel, 94, 202

determination of biogenic amines using heterocyclic cation and aromatic anion elution, 97, 282

determination of glucosamine, galactosamine, and amino acids, 96, 77

sulfur, chromatography on single-column amino acid analyzer, 98, 293

thiohydantoins, chromatographic isocratic resolution, 95, 494

identification and quantitative determination of chitosan oligosaccharides, 95, 563

single-column, chromatography of sulfur amino acids, 98, 293

trinitrobenzenesulfonic acid method for detection in chromatographic column effluents, 96, 3 17

ultramicro analysis, application to studies of protein metabolism in cultured cells, 96, 45

single-column procedure, basement membrane and collagen-like proteins (human, rabbit), 99, 427

Amino acid decarboxylases spectrophotometric estimation of arginine decar-

boxylase (oat seedlings), 92, 33 1 L-Amino acid decarboxylase

voltammetric assay for activity by chromatography, 108, 160

a-Amino acids separation of peptides on copper-Sephadex (cheese,

yeast), 97, 367 N-acetylation of methyl glycosides, 94, 36 fluorometric quantitation in picomole range, 98,

190 Aminoacyl-tRNA

D-Amino acid oxidase isolation from kidney using granulated hydroxy-

apatite (porcine), 94, 105 purification of flavin adenine dinucleotide and its

analogs, 95, 156

column separation and fractionation, 94, 48 -GTP-elongation factor Tu complex, quantitative

assay for formation, 92, 123 ligases, simultaneous measurement of charging

levels, 99, 372

L-Amino acid oxidase -peroxidase system, protease activity measure-

ment (Aspergillus japonica), 97, 11 Amino acids

analysis

m-Aminobenzamidine trypsin purification, porous silica gel as bioad-

sorbent and biocatalyst, 98, 238 p-Aminobenzoic acid

y-carboxyglutamic acid, 92, 466 extraction of proteins and peptides from Coomas-

sie blue-stained sodium dodecyl sulfate-polyacylamide gels, 96, 352

high-performance liquid chromatography (guinea pig), 93, 423

urinary, assay for measurement in oral pancreatic function test (human), 98, 13

p-Aminobenzoylpolyglutamates cleavage from formyl derivatives, 92, 501

a-Aminobutyrate aminotransferase assay by coupling to glutamate decarboxylase

and measuring WO, release (Escherichia coli), 96, 24

nanogram levels of, by reversed-phase high- pressure liquid chromatography, 92, 170

on solid support, 94, 60

I-Aminocyclopropane- I-carboxylic acid quantitative determination, liberation of ethylene,

loo, 140

automated fluorimetric analysis, 94, 313 branched chain, synthesis of radioactive inter-

mediates, 98, 281

8-(6AminohexyB-amino-guanosine-5’-diphosphate synthesis and application as ligand in affinity

chromatography, 99, 189

chromatographic detection in urine, 97, 328 composition, quantitation of proteins, 96, 130 conversion to analogs of ethylenedinitrilotetra-

acetic acid, chelating agents, 108, 152 electrophoretic and chromatographic characteriza-

tion using acidic mobile phases, 100, 254

8-(6Aminohexyl)-amino-guanosine-5’-triphosphate synthesis and application as ligand in affinity

chromatography, 99, 189 5-Aminolevulinic acid dehydratase

rapid purification and direct spectrophotometric assay, 97, 277

Amino-procollagen fluorometric analysis with o-phthalaldehyde, 93,

174 fluorometric analyzer, construction of instrument

using o-phthalaldehyde as detection agent, 96, 298

interfering effect on Lowry method protein assay (review), 100, 201

micromethod analysis by gas chromatography and mass spectrometry, 92, 426

phenylthiohydantoin, chromatographic analysis on DuPont Zorbax cyanopropylsilane columns, loo, 335

peptidase type 1, determination of kinetic parameters and endopeptidase activity (bovine), 95, 406

Aminotransferase asparate, preparation of p-hydroxy-L-asparate using

cysteine sulfinate and dihydroxyfumarate (porcine), 93, 134

u-Aminovalerate fluorometric assay with o-phthalaldehyde, 95, 44

analysis, fluorometric assay for urea in urine, plasma, and tubular fluid, 98, 136


determination by microfluorometric enzymatic assay, 95, 507

Anhydrotrypsin

electrode used in urease assay, 95, 483 -pyridine, use in gel filtration of peptides, 95,

397 Ammonium sulfate

immobilized, biospecific affinity adsorbent for peptides produced by trypsin-like proteases (bovine), 98, 198

Aniline

crystallization of glutathione reductase chromatographically purified from erythrocytes (porcine), 98, 335

specific maltose labeling in reducing glucose moiety (Spinacacia oleracea), 96, 12

stimulation of phospholipase C (Clostridium per- fn’ngens), 97, 43

Amniotic fluid

radioactive, peptidyl derivative as sensitive, stable, protease substrate, 97, 269

Anthranilate synthetase whole-cell assay (Escherichia coli), 96, 152

Antibodies affinity-purified against collagen, radioimmunoas-

say, 96, 489 antiactin, solid-phase assay using protein-A (mouse,

rabbit), 94, 140 measurement of diamine oxidase activity colori-

metrically, fluorometrically, and radiochemi- tally (human), 96, 411

CAMP (adenosine 3’,5’-monophosphate) -dependent protein kinases, estimation of receptor

capacity, 98, 310

antiestradiol, immobilized, set in competition for tritium-labeled estradiol with estradiol receptor, 94, 278

enzyme immunoassay using urease conjugates, 95, 483

phosphodiesterase measurement, 100, 244 CAMP diesterase

separation of enzymatic activities chromatographi- tally (Dictyostelium discoideum), 100, 179

Amperometry determination of chorionic gonadotropin by mem-

brane-bound antibody (human), 94, 22 Ampholytes

carrier, mitogenic and inhibitory effects on quiescent and stimulated diploid lung fibroblasts (human), 98, 27

Amylases

-antigen interaction, effect of detergents, 98, 445 cellulose, release radioimmunoassay for determina-

tion of chorionic somatomammotropin plasma levels (human), 99, 121

cross-reaction with protein and nitrate reductases (Azotobacter vinelandii), 95, 24

-enzyme conjugates, preparation methods and application for enzyme immunoassay, 100, 108

fluoroimmunoassay on lymphoblastoid cell suspensions (human), 94, 176

membrane-bound, amperometric determination of chorionic gonadotropin (human), 94, 22

-peroxidase binding to membrane fragments, chromatographic quantitation (rabbit, human), 98, 53

combined isoelectric focusing, immunoabsorption, and enzymatic characterization (rabbit), 93, 261

Amyloglucosidase

radioimmunoassay, 4x174 DNA.RNA hybrid (Staphylococcus aureus), 98, 74

Antigens -antibody interaction, effect of detergents, 98, 445 enzyme immunoassays using urease conjugates,

95, 483 specific determination of total carbohydrate con-

tent of liver homogenate in single step (rat), 98, 47

Anabasine nitrosation rate constants, radioimmunoassay of

N’-nitrosonomicotine, 95, 387 Anacystis nidulans

fluoroimmunoassay on lymphoblastoid cell suspensions (human), 94, 176

immunologically active, solid-phase assay of (mouse, rabbit), 94, 140

a-1-Antitrypsin removal of concanavalin A, 93, 180

Apovitellenin II preparation of chlorophyll a by nonchromatographic chromatographic separation from egg yolk (chicken),

method, 96, 126 94, 53 Anex Arabinogalactan

OSTION LGAT 0800, high-performance liquid chromatography isocratic separation of DNA bases and deoxyribonucleosides on, 94, 237

Angiotensin

isolation by lectin affinity chromatography, 92, 41

Arene oxides

converting enzyme, spectrophotometric assay, 95, 540

Angiotensin I

K-region, fluorometric assay of epoxide hydrase activity, 97, 340

Arginase

radioimmunoassay of (human), 94, 125 2,5-Anhydrohexoses

assay, urea electrode, 95, 479 L-Arginine

quantitation of glycosaminoglycan hexosamine, 98, 478

-containing peptides, immobilized anhydrotrypsin as biospecific affinity adsorbent (bovine), 98, 198


Arginine decarboxylase spectrophotometric estimation of (oat seedlings),

92, 331 Argininosuccinase

isolation and comparison of catalytic, physical, and chemical properties with enzymes (bovine brain, liver, kidney), 95, 139

Arsenoza III absorbance, effects of changes in pH, 96, 70

N-Arylacetohydroxamic acid quantitative determination in biochemical reaction

mixtures, 98, 169 N-Arylglycolhydroxamic acid

quantitative determination in biochemical reaction mixtures, 98, 169

Arylsulfatase A activity, problems and solutions for detection after

electrophoresis, 97, 36 Ascorbic acid

automated assay of vitamin C in plasma and tissues (rat), 92, 510

spectrophotometric determination, 96, 1 L-Aspartate

:2-oxoglutarate aminotransferase, enzymatic assay, loo, 20

Asparate aminotransferase assay by coupling to glutamate decarboxylase

and measuring WO, release (Escherichia co/i), 96, 24

Aspartate transcarbamylase chromatographic purification from germinated seed-

lings (Phase&s aureus), 95, 401 ATP (adenosine Y-triphosphate)

factors affecting kinetics of light emission from luciferase (firefly), 95, 194

[y-=P]ATP enzymic preparation, free of salt and buffer,

93, 272 5’ labeling of mononucleotides, 93, 26 substrate for assay of 5-phosphoribosyl-cu- l-pyro-

phosphate synthetase activity (Salmonella typhimurium), 98, 254

ATP phosphoribosyhransferase -histidyI-tRNA complex, nitrocellulose filter bind-

ing, 92, 22 ATP pyrophosphohydrolase

separation of enzymatic activities chromatographi- tally (Dicfyosrelium discoideum), 109, 179

ATP sulfurylase analysis of inorganic pyrophosphate using, 94, 117

ATPase activities of myosin and actomyosin inhibited by

free chromophore (rabbit), 95, 317 activity of isolated myocardial mitochondria (canine),

96, 293 fluorimetry using auxilary-coupling enzymes to as-

say ATPase activity (rabbit muscle), 92, 375 isolation and storage of plasma membranes (Neuro-

spora crassa), 9.5, 554

mitochondrial, labeling of hydrolytic site, 95, 39 Autoradiography

apparatus, longitudinal gel slicer for cylindrical gels, 97, 51

color negative films, radioautography of multiple isotopes, 93, 189

detection and quantitation of lactose synthesis and secretion, 94, 340

double-label, comparison of complex protein mixtures after electrophoresis, 96, 39

immunoassay, rapid technique for semiquantitative mass screening of haptens, 92, 147

localization of peptides, 94, 1.50 radioimmunological screening method for specific

membrane proteins (Escherichia co/i), 97, 153 Avidin

monomer affinity column for purification of biotin- containing enzymes, 94, 366

B

Bacteria enter& modified assay procedures for phospho-

transferase system, 95, 293 extraction and measurement of endogenous free

tryptophan (Escherichia cob), 93, 390 inhibition preparation of pyocyanin and unusual

bacterial sensitivity (Pseudomonas aeruginosa, Profeus morganii), 95, 19

systems utilizing oxygenated leghemoglobin and myoglobin as sources of free dissolved 0, at low concentrations (Rhizobium), 96, 165

Bacteriophages @X174, radioimmunoassay of antibody to RNA.

DNA hybrid (Staphylococcus aureus), 98, 74 Barley stripe mosaic virus

size determination of poly(A) sequences in RNA, 99, 450

Bases compositional analysis of nucleic acids, 93, 26 DNA, high-performance liquid chromatography iso-

cratic separation, 94, 237 reversed-phase liquid chromatography, practical

aspects applied to biochemical and biomedical research (review), 99, 1

Benzidine hemoglobin quantification in polyacrylamide gels,

94,211 Benzo[a]pyrene

enzymatic hydration of K-region arene oxides, 97, 340

macromolecular binding, simultaneous preparation of nuclear DNA, RNA, and protein from carcinogen treated embryo fibroblasts (hamster), 97, 410

metabolism, analysis by position-sensing proportional counting of thin-layer chromatograms, 99, 165

metabolites. chromatographic resolution using or-

ganic amines and application to marine me- Blood tabolites (Stenotomus versicolor), 99, 129 calorimetric determination of sulfhydryl content,

N-Benzoyl-L-tyrosyl-p-aminobenzoic acid 93, 98 oral pancreatic test, assay for measurement of differentiator for conversion of spectrophotometer

urinary p-aminobenzoic acid (human), 98, 13 into coagulometer and application for quanti- Bile tation of venom procoagulants (Croralus hor-

determination of free and conjugated acids as ridus horridus, Agkistrodon contortrix moka- ultraviolet-absorbing ion pairs by reverse-phase sen), 97,418 HPLC, 108,260 effect of selenium on determination of mercury

determination of lithogenic index by chromatog- (mouse, rabbit), 98, 287 raphy and densitometry using dual-wavelength endogenous retinoic acid, demonstration in normal chromatoscanner, 188, 146 physiological conditions (human), 98, 402

3-keto acids, enzymatic method for quantitative estimation of dehydroascorbic acid as 2,4-dinitro- determination, 99, 408 phenylhydrazone derivative in diabetic (hu-

Bilirubin man), 98, 368 determination of total and free serum concentra- photochemical determination of carbon monoxide

tions based on static fluorescence quenching levels, 94, 431 of dansyl serum albumin (bovine, human), microassays of bilirubin and bihrubin binding 96,364 capacity (human), 188, 25

fluorometric study of partition, basis for rapid serum microassay of bilirubin and bilirubin binding determination of lactate dehydrogenase (human), capacity in blood (human), 188, 25 97, 255

Binding photometric assay for alcohol dehydrogenase affmity procedure, purification of cytoplasm elonga- activity (equine, human), 99, 65

tion factor 2 and organellar elongation factor solubilization and assay in liquid scintillation count- G (eukaryote), 99, 434 ing (mouse, rat), 99, 288

assay for tetrodotoxin binding to detergent extracts specific adsorption of white cells, preparation of of excitable tissues (Electrophorus electricus), polysomes and mRNA from immature erythro- 99,12 cytes (chicken), 98, 464

fatty acids and dNgs to immobilized albumin, Blue Sepharose studied by chromatography (bovine), 99, 352 chromatographic purification of acid ethanol-ex-

glycoproteins to concanavahn A and agglutinin tracted lymphoid interferons (human), 98, 214 and the dissociation of their complexes (Ri- CLdB, purification of creatine kinase isozymes cinus communis), 99, 254 (Lepomis cyanellus), 94, 89

measurement of anionic sites on surfaces of kidney Bolton-Hunter reagent, see p-Hydroxyphenyl pro- cells using radiolabeled polycationic ferritin pionic acid (hamster), 97, 400 Borate

parameters, determination by protection experiments, 92, 123

complexes, analysis of glycopeptides by electro-

peroxidase-antibody to membrane fragments, chro- phoresis, 97, 438

matographic quantitation (human, rabbit), 98, ion, comparative analysis of interaction with polyols,

53 95, 383

reaction, dissociation of cyclic AMP-dependent Brain

protein kinase, 98, 3 10 bicarbonate tissue measurement (rat), 95, 183

-receptor assay, application of miniature ultra- biochemical determination of tubulin-microtubule

centrifuge, 96, 395 equilibrium in cultured cells (rat), 96, 155

specificity, N-acetyl-n-glucosamine binding lectins, cerebellum, enzymatic method for measurement of

99,221 GTP and GDP in biological samples (mouse),

Bioautography 95, 512

method for detecting L-camitine and its derivatives cerebroside sulfate, rapid cleavage of ester lipids

using mutant (Torufopsis bovina), %, 322 and large-scale isolation (porcine), 100, 364

Biosynthesis chromatographic analysis of histidine-containing urinary oxalate quantitative index, 94, 477 dipeptides, polyamines, and amino acids (guinea

2,2’-Biquinoline pig), 93, 423 -copper (II) solution, reagent for ascorbic acid dinitrophenyl derivatives, gas chromatography-

analysis, 96, 1 chemical ionization mass spectrometry analysis Biuret method of phenylethylamines (rat), 96, 308

reaction, quantitative determination of insoluble discrete regions, fluorometric simultaneous deter- proteins, 95, 340 mination of noradrenaline and 3-methoxy-4-



hydroxyphenylethyleneglycol sulfate (rat), 97, 352

gas chromatography-mass spectrometric characteristics and assay of rele-methylhistamine (rat), %,56

microtubule protein, polymerization kinetics during purification cycles following isolation without glycerol (bovine), 98, 64

purification of microtubule protein and comparison of assembly requirements for neuronal microtubules (bovine, hog), 96, 225

spectrophotometric cycling assay for reduced coenzyme A and its esters (mouse), 97, 103

Bromide cyanogen and tryptic cleavage maps, argininosuc-

cinase (bovine brain, liver, kidney), 95, 139 N-Bromosuccinimide

attachment of tryptophanyl peptides to 3-aminopropyl-glass suited for subsequent solid-phase degradation, 97, 56

Bromosulfophthalein -glutathione conjugate covalently linked to Sepharose

4B, affinity chromatography of albumin (rat plasma), 92, 290

Buffers Good’s, free metal ion depletion, 95, 527 interfering effect on Lowry method protein assay

(review), 180, 201 sodium dodecyl sulfate-mercaptoethanol-tris elec-

trophoresis buffer, quantitation of solubilized proteins, 100, 64

Cadmium digestion of muscle biopsies for trace analysis

using organic tissue solubilizer (human), 98, 315

Cadmium chloride reducing agent, effect of selenium on determina-

tion of mercury in tissues (mouse, rabbit), 98, 287

Calcium -binding ability, purification of regulator of cyclic

nucleotide phosphodiesterase from liver (rat), 96, 407

binding chelates, chemical and spectral characterization of extrapallial fluid (Mytilus eddis), 100, 324

effects of changes in pH on absorbance of ar- senazo III, 96, 70

electrode, kinetics of mitochondrial transport (rat), 93, 430

ion concentration in presence of magnesium and

chelating effecters of NAD+-linked isocitrate dehydrogenase, 95, 62

depletion, affinity constants for 1:l complexes with N-(2-acetamido)iminodiacetic acid, 95,527

metabolism, y-carboxyglutamate analysis by selective anion exchange elution and fluorescent detection, 98, 219

mitochondrial transport, electrode for studying kinetics, 93, 430

selective electrode, measurement of ion activities, 97, 315

uptake by microsomes, cardiac sarcoplasmic reticulum isolation from heart (bovine), 99, 41

Calorimetry assay for hormone- and metabolite-induced changes

in steady-state heat production by tissue (Zea mays), 100, 77

L-Canaline radiometric determination using [2-Wlacetone as a

reagent, 92, 265 Canavalia ensiformis

radiometric determination of L-canaline using [2- W] acetone as a reagent, 92, 265

Cancer carcinogens, radioimmunoassay of N ‘-nitrosonor-

nicotine (rabbit), 95, 387 micro determination of polyamines with oxidase

(snake venom), 92, 453 Carbamazepine

quantitative determination in brain areas by multiple detection mass fragmentography (rat), 93, 115

Carbamazepine- 10,l I-epoxide quantitative determination in brain areas by multiple

detection mass fragmentography (rat), 93, 115 Carbamoyl disulfides

chromatographic quantitative analysis of deprotection of dithiasuccinoyl amino acids, 95, 160

S-Carbamoylmethylcysteine reduction of sulfoxides in peptides and proteins,

98, 36 Carbamylation

heat-induced, electrophoretic behavior of proteins during, 93, 312

Carbazole method modified

determination of uranic acid in vegetable fiber preparations, 96, 282

hexuronic acid quantitation, 93, 295 Carbohydrates

fluorometric determination of trioses with o-phenylphenol, 94, 1

isotachophoresis for periodate oxidation analysis, 98, 417

separation by reverse-phase high-pressure liquid chromatography, 97, 184

separation of glycoprotein-derived oligosaccharides by thin-layer chromatography, 93, 167

specific determination of total content of liver homogenate in single step, use of purified amyloglucosidase (rat), 98, 47

Carbon 14 -containing polyacrylamide gel slices, one-step liquid

scintillation counting method, 99, 161


Carbon dioxide

bicarbonate tissue measurements (rat liver, brain,

heart, and skeletal muscle), 95, 183

“‘CO,/‘“CO,, spectroscopic method for measurement

(rat), 96, 64

continuous and discontinuous photometric analysis

in gases and fluids, 92, 255

radioactive. unified assay of enzymes of glutamate

metabolism, 96, 24

Carbon monoxide

continuous and discontinuous photometric analysis

in gases and fluids, 92, 255

determination in blood, 94, 43 1

y-Carboxyglutamate

analysis by selective anion exchange elution and

fluorescent detection, 98, 219

y-Carboxyglutamic acid

analysis via alkaline hydrolysis, 92, 466

micro determination of polyamines with oxidase

(snake venom), 92, 453

Carboxymethyldextran

concentration of proteins, 98, 353

L-Carnitine

chromatographic and bioautographic method for

detection utilizing mutant (Tordopsis hovinu),

96, 322

Catalase

trehalose hydrolysis coupled to dioxide consump-

tion, 94, 360

Catalyst

activity, solid phase radioimmunoassay for lipo-

protein lipase (chicken), 94, 346

Catechol

siderophores, chromatographic isolation (Micro-

cocc~.~ denitrijwns, Klehsiello uerogenes,

Bacillus suhtilis), 95, 364

Catechol O-methyltransferase

assay by determination of m- and p-O-methylated

products using chromatography, 98, 452

Catecholamines

reversed-phase liquid chromatography, practical as-

pects applied to biochemical and biomedical

research (review), 99, I

Cells

adhesion, fibronectin- and phosphate-mediated at-

tachment to collagen substrates, 94, 308

culture

biochemical determination oftubulin-microtubule

equilibrium (rat, human), 96, 155

fluorometric analysis of DNA and protein (rat),

94, 166

mitogenic and inhibitory effects of carrier ampho-

lytes on quiescent and stimulated diploid lung

libroblasts (human). 98, 27

disruption by nitrogen cavitation, isolation of intra-

cisternal type A-particles and associated RNA

(mouse), 97, 85

fractions, electrophoretic analysis of basic proteins

using phosphatidyl choline/urea solubilization,

100, 289

separation, application of nonsynchronous flow-

through coil planet centrifuge, 94, 249

silkworm eggs and hatched worms, atomic ab-

sorption spectrometry for trace metal analysis,

94, 386

-surface interactions, methodology allowing stud-

ies in situ, 92, 74

thin-layer, spectroelectrochemical, construction and

characterization, 93, 366

walls, determination of aldoses and uranic acid

content of vegetable fiber, 96, 282

Cellulase

activity determined by gas-liquid chromatography

(ovine), 92, 270

Cellulose

-DEAE, removal of concanavalin A from o-l-

antitrypsin. 93, I80

dihydroxyboryl-substituted, chromatographic puri-

fication of nucleosidyl peptides (rabbit), 99,

85

mercurated, interaction with 6-thiopurines and thiol-

containing RNA, 92, 324

Cellulose acetate

properties of liquid polyacrylamide in the presence

of, 92, 99

Centrifugation

apparatus

column for desalting protein solutions, 100, I84

horizontal flow-through coil planet, countercur-

rent chromatography, 100, 271

nonsynchronous flow-through coil planet, applied

to cell separation with physiological solution,

94, 249

zonal, floating device for top fractionation of

density gradients, 97, 72

elutriation, resolution of erythrocytes (human),

98, 112

Ceramides

chromatographic-spectrometric estimation of

sphingolipids (human), 97, 323

Cerebroside sulfate

brain, rapid cleavage of ester lipids and large-

scale isolation (porcine), 100, 364

Cesium sulfate

isopycnic centrifugation, simultaneous preparation

of nuclear DNA, RNA, and protein from

carcinogen treated embryo fibroblasts (ham-

ster), 97, 410

Cetyltrimethylammonium bromide polyacrylamide

electrophoretic estimation of protein subunit molec-

ular weights, 92, 41 I

Chaetoglobosins

interaction with actins or cytochalasins (rabbit

muscle), 95, 14 enzyme stabilization by immobilization (review), Chelates

93, I calcium binding, chemical and spectral characteri-

zation of extrapallial fluid (Mytilus edulis), 100, 324

catechol-type, chromatographic isolation of siderophores, 95, 364

conversion of amino acids to analogs of ethylenedinitrilotetraacetic acid, 100, 152


metal ion and complex formation, spin-label assay, 99, 175

NAD+-linked isocitrate dehydrogenase, calcium ion concentration, 95, 62

zinc chromatography, purification of plasma a2 macroglobulin and a, proteinase inhibitor (human), 99,415

Chemiluminescence enzyme immunoassay of cortisol using peroxidase

as label, 97, 248 luminol-immunoglobulin G conjugate (human), 93,

267 Chemotherapy

drugs, pre-clinical testing, 95, 329 Chenodeoxycholate

conjugates, enzyme immunoassay, 93, 361 Chironomus fenfans

electrophoretic selection and fractionation of poly(A)-containing RNA and poly(A), 92, 280

Chitosan oligosaccharides, identification and quantitative de-

termination using amino acid analyzer, 95, 563

Chloramine-T estimation of methionine amounts by reaction with,

93, 419 Chloramphenicol

tritium-labeled, chromatographic preparation, 96, 215

Chlorides metal, vaporization characteristics, 95, 8

Chloroform extraction, removal of Triton X-100 from protein

solution, 97, 116 heptane:methanol, calorimetric determination in

subcellular organelles, 97, 403 Chlorophyll a

nonchromatographic preparation method (Anacystis nidulans), 96, 126

Cholesterol epoxide hydrase, radiochromatographic assay, 94,

383 reduced cholesterokphospholipid molar ratios in

cell membranes (human), 95, 344 Cholesteryl 4(3’-pyrenyl)butanoate

incorporation into low-density lipoproteins, 92, 74 Chromatin

lysine-phosphate bonds, reactions of fluorescamine with chromosomal proteins (herring, salmon), 99, 274

micrococcal nuclease digested for fractionation and purification of monomeric nucleosomes, 92,420

Chromatography apparatus; countercurrent, horizontal flow-through

coil planet centrifuge, 100, 271 ascending, improved rapid assay of glycogen syn-

thase, 100, 371 Blue Sepharose

purification of acid ethanol-extracted lymphoid interferons (human), 98, 214

cibacron blue- Sepharose 4B pseudoaffinity, used for preparation of phosphoglycerate kinase (Sac- charomyces cerevisiae), 93, 46

covalent, purification of procollagen type II by, 94, 253

O-(diethylaminoethyl)-cellulose isolation of vitellogenin from plasma (Xenopus

laevis, vertebrates), 97, 145 removal of concanavalin A from o- I-antitrypsin,

93, 180 differentiation of nicotinamide riboside and l-

methylnicotinamide, 93, 384 enzymatic preparation of 3’-phosphoadenosine-5’-

phosphosulfate, 96, 34 exclusion, estimation of peptide molecular weight,

94, 228 gel-filtration, separation of soluble proteins from egg

yolk (chicken), 94, 53 high-pressure gel permeation, separation of enzy-

matic activities (Dictyosfelium discoideum), loo, 179

polyacrylamide gel, separation and identification of pteroylpolyglutamates, 92, 501

quantitative analysis of deprotection of dithiasuccinoyl amino acids, 95, 160

removal of DNA from RNA on acetylated N-[N’- (m - dihydroxylborylphenyl)succinamyl]amino - ethyl cellulose, 100, 174

subunit-exchange behavior of cY-chymotrypsin, 95, 89 spectrophotometric determination of myoglobin

in cardiac and skeletal muscle, 92, 473 two-dimensional, detection and quantitation of

lactose synthesis and secretion, 94, 340 zinc chelates, purification of plasma LYE macro-

globulin and (I, proteinase inhibitor (human), 99, 415

Chromophores blue copper, preparation and characterization of

chemically modified plastocyanin, 95, 254 intramolecularly quenched fluorogenic substrates

for hydrolytic enzymes, 95, 228 merits of leaving groups in assay for serine pro-

teases, substrates for leukocyte and pancreatic elastase (human, porcine), 99, 53

Chromosomes proteins, reactions of fluorescamine (herring, salmon),

99, 274 Chrondroitin sulfates



isomeric, chromatographic determinations of disaccharides resulting from enzymatic degradation, 94, 231

Chronoamperometry determination of homogeneous small molecule-

redox protein reaction rates, 96, 326 Chymotrypsin

immobilized, porous silica gel as bioadsorbent and biocatalyst, 98, 238

kinetics of albumin digestion, application of protein separation by molecular weight (bovine), 98, 410

new substrate synthesized, succinyl-Ala-Ala-Pro- Phe-p-nitroanilide, 99, 3 16

a-Chymotrypsin behavior, subunit-exchange chromatography, 95,89

Cibacron blue differential binding of contractile proteins from

skeletal muscle (rabbit), 95, 3 17 removal of human serum albumin from, 92, 144 -Sepharose 4B pseudoaffinity chromatography used

for preparation of phosphoglycerate kinase from Saccharomyces cerevisiae, 93, 46

Circular dichroism kinetic assay for mandelate racemase using, 94,

329 Citric acid

-detergent, isolating nuclear and cytoplasmic fractions containing undegraded RNA from cells (Xenopus laevis), 100, 166

Citrovorum factor radiochemical assay for Wformyltetrahydrofolic

acid, 94, 483 Cobalt

ion depletion, affinity constants for 1:1 complexes with N-(2-acetamido)-iminodiacetic acid, 95, 527

Coenzyme A affmity column, purification of phosphotransacetyl-

ase (Clostricium kluyveri), 95, 2 determination chromatographically and application

in assay of long-chain acyl-CoA derivatives, 96, 181

total tissue level, spectrophotometric cycling assay (mouse), 97, 103

Colchicine binding, biochemical determination of tubulin-

microtubule equilibrium in cultured cells, 96, 155

Collagen [I-“Clacetylated, assay for collagenase, 99, 340 -like proteins; accelerated, single-column procedure

for amino acid analysis (human, rabbit), 99, 427

Collagenase assay method based on p-dioxane, 92, 136 assay using [I-i*C]acetylated collagen, 99, 340 -like peptidase activity, fluorescence assay, 180,

43

Collagens analysis of cyanogen bromide peptides using elec-

trophoresis, 92, 183 direct visualization in polyacrylamide gels, 97,

359 iodination for use in radioimmunoassay, 96, 489 material, separation of procollagen by covalent

chromatography, 94, 253 quantitative estimation based on binding of Sirius

Supra red F3BA, 94, 96 substrates, fibronectin- and phosphate-mediated at-

tachment of ovary cells (Chinese hamster), 94; 308

synthesis, radiolabeled assay in microwell fibroblast cultures, 96, 220

Colorimetry analysis of ureido compounds, 97,421 assay for nanomole amounts of inorganic phos-

phate, 188, 95 determination of erythrocyte surface siahc acid

residues, 97, 346 determination of long-chain free fatty acids in sub-

cellular organelles, 97, 403 determination of total suhhydryl content of whole

blood and plasma, 93, 98 discriminating between galactosamine and ghtco-

samine in mixtures, 94, 394 measurement of diamine oxidase activity in amniotic

fluid (human), 96, 411 significance of sodium hydroxide in determination

of protein, 108, 98 trypsin-like enzymes, substrate thiobenzl benzyl-

oxycarbonyl+lypsinate, 93, 223 Column chromatography

aminoacyl tRNA, 94, 48 0-(diethylaminoethyl)-celluloseSephace1, separa-

tion of mannosylretinylphosphate from dolichylmannosylphosphate (rat), 97, 298

effluents, trinitrobenzenesulfonic acid method for monitoring amino acids and peptides, 96, 317

isolation of catechol-type siderophores (Micrococ- cus denitrificans , Klebsiella aerogenes , Bacillus subtilis), 95, 364

peroxidase-antibody binding to membrane fragments quantitated (human, rabbit), 98, 53

polyacrylamide beads, histones to determine separation factors (cricket), 94, 220

purification of nucleosidyl peptides on dihydroxyboryl-substituted polyacrylamide and cellulose (rabbit), 99, 85

RPC-5 at high pH, fractionation of dG homooligonucleotides, 99, 213

separation of $*P, from [Y-~*P]ATP, 93, 272 Sepharose 4B

fractionation of isoaccepting tRNA species (Esch- erichia coli), 93, 248

separation of androgen receptor from testosterone- binding globulin, 92, 164


specific maltose labeling in reducing glucose moiety (Spinacacia oleracea), 96, 12

Computers laser spectrophotometer with interactive-graphic

data analysis, 96, 456 programs, spectrometric assay for sulindac and

its metabolites in plasma (human), 95, 579 spectrometer, simultaneous measurement of fluores-

dermis tyrosinase (Rana escufenta ridibunda), 95,433

Cyanogen bromide digestion of cytochromes, estimation of peptide

molecular weight, 94, 228 fluorescent labeled, gel electrophoresis of cleavage

products, 95, 122 y-Cyanogen bromide

cence and phosphorescence using synchro- peptides, separation of fetal hemoglobin chronously gated photon counters, 97, 158 matographically (human), 96, 113

Concanavalin A Cyanopropylsilane binding studies, quantitation and differentiation of DuPont Zorbax columns, chromatographic analysis

lectin-glycoprotein complexes by laser ne- of phenylthiohydantoin amino acids, 100, 335 phelometry, 96, 391 Cyclic nucleotides, see Nucleotides

factors affecting binding of glycoprotein and the Cyclooxygenase dissociation of their complexes, 99, 254 products, determination using high-performance

radiometric assay for 5’-nucleotidase, 95, 497 liquid chromatography and radioimmunoassay, removal of from a-1-antitrypsin, 93, 180 93, 339

Coomassie blue Cysteine prestaining proteins, 93, 373 histone H3, dithiobis-(nitrobenzoic acid induced properties as proteins stain and dye impurities inter- and intramolecular crosslinks, 94, 465

and effect on protein-dye binding with gels, thioether-linked residues, determination of in cyto- %, 262 chromes c and phycobiliproteins, 92, 489

-stained sodium dodecyl sulfate-polyacrylamide gels, extraction of proteins and peptides, 96, 352

Copper (II)-2,2’-biquinoline solution, reagent for ascorbic

acid analysis, 96, 1 digestion of muscle biopsies for trace analysis

using organic tissue solubilizer (human), 98, 315

estimation of proteins in colored or turbid solutions, 100, 92

gelatin films analyzed by secondary ion mass spectrometry, 92, 213

ion depletion, affinity constants for 1: 1 complexes with N-(2-acetamidohminodiacetic acid, 95, 527

-Sephadex, separation of peptides from a-amino acids (cheese, yeast), 97, 367

soaps of free fatty acids, calorimetric determination in subcellular organelles, 97, 403

Cortisol chemiluminescence enzyme immunoassay using per-

oxidase as label, 97, 248 Coulometry

characterization of thin-layer spectroelectrochemical cell, 93, 366

electrochemical method for measuring redox potentials of low potential proteins, 99, 399

Creatine kinase isozymes, purification with blue Sepharose CL-6B

(Lepomis cyan&us), 94, 89 Creatine phosphokinase

myocardial, effect of sodium desoxycholate on extraction (rat), 98, 278

Cresolase activity, spectrophotometric determination in epi-

Cysteine sulfinate used for preparation of diastereoisomers of /3-

hydroxy-L-aspartate with heart aspartate aminotransferase (porcine), 93, 134

Cytidine determination of ribonuclease (pancreatic) activity

in serum (human), 100, 233 Cytochalasans

interaction between actin and chaetoglobisins (rabbit muscle), 95, 14

Cytochrome c attachment of tryptophanyl peptides to 3-amino-

propyl-glass suited for subsequent solid-phase degradation (equine), 97, 56

determination of thioether-linked cysteine, 92, 489 preparation of partial cyanogen bromide digestion,

94, 228 preparation of subunits of flavocytochromes c

(Chlorobium limicola thiosulfatophilum, Chro- matium vinosum), 95, 209

reaction with ferrous-EDTA, comparison between stopped-flow kinetic and chronoamperometric kinetics and as function of ionic strength (equine, Rhodospirillum rubrum), 96, 326

Cytochrome P-450 fractionation (mouse liver), 95, 201 -mediated acetanilide 4-hydroxylase activity, limit

of sensitivity (mouse), 96, 201 Cytokinins

determination by mass spectrometry, 92, 111 Cytoplasm

detergent-citric acid technique for isolating fractions containing undegraded RNA from cells (Xenopus [aevis), 100, 166

purification of elongation factor 2 and organellar

elongation factor G by affinity binding pro- chondroitin sulfates, based on chromatographic cedure (eukaryote), 99, 434 separations and disaccharide products, 94, 23 1

Cytosine Detergents 5methylcytosine ratio, quantitation in DNA, 94,

297

D

Dansylation protein visualization, simultaneous isolation of major

viral proteins electrophoretically, 98, 329 Dansylcadaverine

cationic, electrophoretic estimation of protein subunit molecular weights using cetyltrimethylammonium bromide polyacrylamide, 92, 411

-citric acid, isolating nuclear and cytoplasmic fractions containing undegraded RNA from cells (Xenopus laevis), 100, 166

determination of protein by modified Lowry procedure, 96, 21

staining for transamidating enzymes, 93, 453 3-Deazaadenosylhomocysteine

inhibition of guanidoacetate methyltransferase, 95, a7

Degradation microbial, enzyme stabilization by immobilization

(review), 93, 1

effect on antibody-antigen interaction, 98, 445 effect on disk assay for determination of glyco-

protein glycosyltransferases (human), 98, 95 enzyme activation, fluorometric assay for UDPglu-

curonyltransferase using 3-hydroxybenzo(a)pyrene as substrate, 98, 394


Dehydroascorbic acid estimation in blood of diabetic by isolation as

2,4-dinitrophenylhydrazone derivative (human), 98, 368

Densitometry

ionic, removal from proteins, 93, 153 non-ionic, removal of Triton X-100 from protein

solution, 97, 116

apparatus, low-cost polyacrylamide gel scanning densitometer, 100, 15

determination of lithogenic index of bile using dual-wavelength chromatoscanner, 100, 146

high resolution gel apparatus based on Zeiss PMQ II microcuvette chamber, 95, 520

Density

solubilization, assay for tetrodotoxin binding to detergent extracts of excitable tissues (Elec- trophorus electricus), 99, 72

zwitterionic and nonionic, electrofocusing of membrane proteins, 95, 201

Deuterium -hydrogen exchange by ultraviolet spectropho-

tometry, 92, 517 Deuterium oxide

ultracentrifugation, initial density related to equilibrium density, cesium chloride and rubidium chloride, 94, 193

Density gradients

extraction and measurement at tracer levels in biological fluids, 98, 208

Diabetes mellitus

floating device to permit fractionation from top, 97, 72

zonal electrophoresis of proteins, 93, 41 Densonucleosis virus

solubilized proteins, purification and peptide mapping (Galleria mellonella), 99, 97

Deoxyguanosine 5’-diphosphate (dGDP) nucleotide substrate, determination of phospho-

enolpyruvate carboxykinase activity, 96, 279 3-Deoxyoctulosonic acid

thiobarbituric acid method for measuring, sialic acid interference, 100, 118

Deoxyribodipyrimidine photolyase purification of photoreactivating enzyme (yeast),

95, 77

estimation of dehydroascorbic acid in blood by isolation as 2,4-dinitrophenylhydrazone derivative (human), 98, 368

1,2-Diacylglycerol levels quantitated using diacylglycerol kinase (Esch-

erichia coli), 98, 123 Diacylglycerol kinase

quantitation of 1 ,Zdiacylglycerol picomole levels (Escherichia coli), 98, 123

N,N’-Diallyltartardiamide cross-linked with sodium dodecyl sulfate-poly-

acrylamide gel properties, macromolecules isolated, 93, 275

Dialysis

Deoxyribonucleoside triphosphate pools in lymphoma cells (mouse), 99, 268

Deoxyribonucleosides DNA, isocratic separation by high-performance

liquid chromatography, 94, 237 Deoxyribonucleotides

chromatographic assay in cell extracts, 99, 268 Depolymerization

mitogenic and inhibitory effects of carrier ampholytes on quiescent and stimulated diploid lung fibroblasts (human), 98, 27

quantitative, improved micro technique, 94, 109 Diamine oxidase

activity, analysis in amniotic fluid calorimetrically, fluorometrically, and radiochemically (human), 96, 411

Diastereoisomers B-hvdroxv-r-asnartate. nreoaration of from cvsteine


CUMULATIVE

sultinate and dihydroxyfumarate with heart aspartate aminotransferase (porcine), 93, 134

4,4’-Diazidostilbene-2,2’-disodium sulfonate crosslinking of proteins, enzyme-polymer film, 97,

320 Diazobenzyloxymethyl

paper, RNA hybridization to labeled DNA, 98, 358

Diazomethane preparation of methyl esters, chromatographic

analysis of abscisic acid (potato), 97, 331 3-Diazonium-1,2,4-triazole

reaction with tryptophan at acid pH, scintillation method for tryptophan determination in proteins, 99, 464

5H-Dibenzo[b,f]azepine-5carboxamide, see Car- bamazepine

2,6-Dichlorophenolindophenol nonenzymatic reduction by phenazine methosul-

fate, 99, 112 O-(Diethylaminoethyl)-cellulose

chromatographic isolation of vitellogenin from plasma (Xenogus laevis, vertebrates), 97, 145

disk assay for photoreactivation of pyrimidine dimers in [3H]DNA (Escherichia co& 97, 376

removal of concanavalin A from a-1-antitrypsin, 93, 180

Diethylstilbestrol metabolites, separation by high-pressure liquid

chromatography, 92, I99 Diffusion coefficient

P-lactoglobulin B, estimation from continuous optical scanning in polyacrylamide gel electrophoresis, 97, 120

two-cross immunodiffusion in body fluids, 93, 103 Digestion

alcoholic alkaline, analysis of ol-tocopherol in liver (ovine), 97, 264

muscle biopsies for trace metal analysis using organic tissue solubilizer (human), 98, 315

Digitonin rapid release of cytoplasmic enzymes from cul-

tured cells, 95, 270 Digoxin

rapid technique for semiquantitative mass screening by autoradiographic immunoassay, 92, 147

Dihydrofolate -Sepharose affinity column, purification of guano-

sine triphosphate cyclohydrolase I and dihydrofolate reductase, 100, 122

Dihydrofolate reductase purification on dihydrofolate-Sepharose affinity

column, 180, 122 Dihydroxyborylphenyl group

chromatographic purification of nucleosidyl peptides on dihydroxyboryl-substituted polyacrylamide and cellulose (rabbit), 99, 85

SUBJECT INDEX 389

Dihydroxyfumarate used for preparation of diastereoisomers of /3-

hydroxy-L-aspartate with heart aspartate amino- tranferase (porcine), 93, 134

3,4-Dihydroxyphenylacetic acid specific activity measurement in rat striatum, 92,

82 la,25-Dihydroxyvitamin D3

-receptor complex, from intestinal mucosa quantitation by steroid receptor assay (chicken), 92, 314

Diiodosulfanilic acid diazotized, isolation of adipocyte plasma mem-

branes, 96, 236 Dimethyl sulfoxide

solutions, quantitation and interaction of glycosaminoglycans with Alcian blue, 93, 60

p-Dimethylamino cinnamaldehyde assay for measurement of urinary p-aminobenzoic

acid in oral pancreatic function test (human), 98, 13

7,12-Dimethylbenzo[a]anthracene enzymatic hydration of K-region arene oxides,

97, 340 metabolism, analysis by position-sensing propor-

tional counting of thin-layer chromatograms, 99, 165

Dimethylnitrosamine chromatographic determination of 7-methylguanine

in hepatic DNA (rat), 97, 203 16,16-Dimethyl-prostaglandin E2

quantitative analysis from plasma using deuterated carrier and gas chromatography-mass spectrometry, 108, 109

2,4-Dinitrophenylhydrazone derivatives of glutamic acid y-semialdehyde and

N-acetyl-t-glutamic acid y-semialdehyde, isolation and characterization, 96, 94

separation by high-pressure liquid chromatography, 92, 31

Dioxane aqueous, isolation of foldback DNA utilizing

nuclease Sl digestion, 96, 144 collagenase assay method based on use of, 92, 136 electrolytes, isotachophoresis for periodate oxida-

tion analysis of carbohydrate, 98, 417 Dipeptides

histidine-containing, analysis by high-performance liquid chromatography (guinea pig), 93, 423

trimethylsilylated, identification with chemical ionization mass spectrometry, 92, 525

Diphenyl oxazole fluorographic detection of radioactivity in poly-

acrylamide gels with water-soluble fluor, sodium salicylate, 98, 132

Disaccharides resulting from enzymatic degradation of chon-


droitin sulfates, chromatographic determinations, 94, 231

Dithiasuccinoyl

nucleotide sequence, approaches to analysis (review), 98, 243

protecting group for amines, chromatographic quantitative analysis, 95, 160

5,5’-Dithiobis(2-nitrobenzoic acid) induced inter- and intramolecular crosslinks in

histone H3, 94, 465

~$29, separation of segments from proteins using glass filters, 93, 158

photolyase, purification of photoreactivating enzyme (yeast), 95, 77

preparative fractionation by agarose gel electrophoresis, 95, 174

leukocyte and pancreatic elastase, merits of chromophoric and fluorogenic leaving groups in assays for serine proteases (human, porcine), 99, 53

2-nitrobenzoic acid, molar absorption coefficient, 94, 75, erratum, 99, 231

recycling analysis of NADP and NADPH, 99, 297

DNA base composition of nanogram quantities, 93, 26 bases, deoxyribonucleosides, isocratic separation

of, by high-performance liquid chromatography, 94, 237

preparative gel electrophoresis apparatus for large scale separations, 100, 304

purification and characterization using cesium chloride gradients and vertical rotor (Escherichia coli, Tetrahymina thermophila), 97, 1%

quantitation for localization in metrizamide gradients (Escherichia cob), 93, 306

quantitative assay, modification using adriamycin (bovine), 95, 559

radioactive, DEAE disk assay for photoreactivation of pyrimidine dimers (Escherichiu co& 97, 376

binding of fluorescent stains, 95, 329 -bisbenzimidazole, nanogram assay in cellular ho-

mogenates by fluorescence, 92,497 cell culture, automated fluorometric analysis (rat),

94, 166

reassociative kinetics, fluorometric measurement (rat), 94, 121

cellular damage, quantitative hydroxylapatite batch assay (human, mouse), 97, 77

chromatographic analysis of I-methylguanine (bovine, Escherichia coli, rat), 97, 203

chromatographic removal from RNA on acetylated iV-[N’-(m-dihydroxylborylphenyl)succinamyl]aminoethyl cellulose, 100, 174

detectection of 5-methylcytosine and quantitation of 5-methylcytosine/cytosine ratio in, 94, 297

-diamidinophenylindole fluorescence, nanogram assay in cellular homogenates by fluorescence, 92, 497

relaxed closed circular, determination in presence of superhelical DNA (simian), 93, 346

replicating polyoma sequence, hybridization method for quantitation (Haemophilus parainfluenzae), 96, 378

restriction fragments, fractionation of dG homooligonucleotides, 99, 2 13

.RNA hybrid, radioimmunoassay of antibody to g%X174 (Staphylococcus aureus), 98, 74

sequencing, application of high-voltage polyacrylamide gel electrophoresis, 100, 129

single-stranded, technique for relating long-range base pairing (simian virus 40), 95, 108

small-volume cuvette for high-resolution thermal denaturation, 97, 136

fluorimetric determination using ethidium dimer, 94, 259

high molecular weight, recovery after electrophoretic separation of low gelling temperature agarose gels, 98, 305

foldback, isolation utilizing nuclease Sl digestion in aqueous dioxane, 96, 144

foreign, detection in plasmids (Escherichia co/i), 98, 60

synthesis, mitogenic and inhibitory effects of carrier ampholytes on quiescent and stimulated diploid lung fibroblasts (human), 98, 27

treated with chemical carcinogens, chromatographic analysis of methylated purines (rat), 100, 58

DNase

inhibition of nuclease contaminant in alkaline phosphatase (Escherichia coli), 95, 458


rapid high-yield isolation method for nuclear envelope ghosts (Tetrahymena pyriformis), 100, 282

Dolichylmannosylphosphate chromatographic separation of mannosylretinyl-

phosphate (rat), 97, 298 Dopa

interpretation of nitrocellulose filter assays of protein-nucleic acid binding, 93, 204

length, electrophoretic measurement, 100, 319 microfluorometric determination in biological mate-

rial using 33258 Hoechst, 100, 184 nanogram assay in cellular homogenates by fluores-

cence, 92, 497

enzymatically formed, fluorescence assay based on isolation (brain), 93, 82

Dopamine metabolites, specific activity measurement in rat

striatum, 92, 82 rapid radioenzymatic assay (gastropod& %, 175

Doxorubicin nuclear, preparation from carcinogen treated em-

bryo fibroblasts (hamster), 97, 410 determination by high-pressure liquid chromatog-

raphy, 94, 112


Drugs binding to immobilized serum albumin chromato-

graphic study (bovine), 99, 352 interfering effect on Lowry method protein assay

(review), 100, 201 metabolism, preparation of intestine microsomal

fractions for studying (rat), 93, 73 Dyes, see Staining

E

Edman degradation automated, large sequencer cup and high-speed

drive, 99, 242 solid-phase, attachment of tryptophanyl peptides

to 3-aminopropyl-glass, 97, 56 Egg

yolk, visualization of proteins after isoelectric focusing during two-dimensional gel electrophoresis (Smitria spec.), 98, 81

Elastase leukocyte and pancreatic, study of merits of various

chromophoric and fluorogenic leaving groups in assay for serine proteases (human, porcine), 99, 53

Elastin biosynthesis, valyl-proline as an index of, 92, 61

Electrical activity heterocyclic cation and aromatic anion elution,

determination of biogenic amines, 97, 282 lipid-impregnated filters as study tool for electric

current-generating proteins, 96, 250 measurement of anionic sites on surfaces of kidney

cells using radiolabeled polycationic ferritin (hamster), 97, 400

measurement of ion activities with solvent polymeric membrane electrodes, 97, 3 15

proton production, simultaneous measurement with aggregation, secretion, oxygen uptake, and intracellular metabolites in platelet suspension, 97, 387

radioactive cations, preparation of lipid vesicles, 94, 302

selective anion exchange elution and fluorescent detection, analysis of y-carboxyglutamate, 98, 219

simultaneous measurement of fluorescence and phosphorescence using synchronously gated photon counters, 97, 158

static charge, reduction in lyophilized materials, 92, 128

Electrochemistry measuring redox potentials of low potential pro-

teins by microcoulometry at controlled potentials, 99, 399

quantitation and characterization of metallothioneins, 100, 343

reduction of pteroylpentaglutamate yielding fully reduced tetrahydro derivative, 97, 48

Electrofocusing membrane proteins of zwitterionic and nonionic

detergents, 95, 201 preparative flatbed, in granulated gels with natural

pH gradients generated from simple buffers, 97, 95

Electrolytes dioxane, isotachophoresis for periodate oxidation

analysis of carbohydrate, 98, 417 Electron probe

X-ray microanalysis of biological specimens, sources of silicon peaks (review), 94, 100

Electron microscopy sarcolemmal purity, isolation of myocardial sub-

cellular organelles by ultracentrifugation (canine), 96, 293

Electrophoresis agarose gel

preparative fractionation of nucleic acids, 95, 174

staining of glycoproteins with fluorescent lectins, 96, 208

apparatus, advantages of elution chamber sand- wiched between gel column halves, 92, 205

argininosuccinase, ioslation and comparison of catalytic, physical, and chemical properties (bovine brain, liver, kidney), 95, 139

complete separation of all known animal glycosaminoglycans in monodimensional run, 99, 311

continuous-pore gradient slabs used without special equipment, 92, 91

desorption of serum albumin from cibacron blue (human), 92, 144

high voltage on neutral paper, prediction of oligo- nucleotide mobilities, 98, 8

isolation and purification of type I procollagen by adsorption to glass beads, analysis of Tris elution fraction (human), 96, 433

isolation of (Y and y subunits of submaxillary gland nerve growth factor (mouse), 92, 482

mobility of macromolecules, interferometric determination technique, 96, 355

mobility of membrane fragments on a sucrose gradient (Halobacterium halobium), 92, 228

multisample separation of animal glycosaminoglycans, 93, 37

preparative recovery of intact high molecular weight RNA

and DNA, 98, 305 slab gel, separation of high-molecular-weight spec-

trin bands, 92, 130, erratum, 95, 587 processing of multiple polyacrylamide gel slabs,

99, 105 quantitative comparative analysis of complex two-

dimensional electropherograms, 97, 210 reagents, storage and delivery using soluble hydro-

colloid films, 95, 549 selection and fractionation of poly(A)-containing

RNA and poly(A), 92, 280


semipreparative, with prestained proteins, 93, 373 separation of labeled protein material, 93, 275 thin-layer, characterization of amino acids using

acidic mobile phases, 100, 254 two-dimensional

biotin-containing, avidin monomer affinity column for purification, 94, 366

analysis of basic proteins using phosphatidyl choline/urea solubilization, 100, 289

analysis of urinary proteins (human), 95, 48 protein heat-induced carbamylation, 93, 3 12 separation of small-molecular-weight proteins, 99,

33

chemiluminescence immunoassay of cortisol using peroxidase as label, 97, 248

electrode-based kinetic assay of activities, 95, 479 fluorometric assay for determination of ammonia,

95, 507 homogeneous, immunoassay of chenodeoxycholate

conjugates, 93, 361

zonal, separation of proteins on density gradients, 93, 41

Electroplax

hydrolytic and proteolytic, intramolecularly quenched fluorogenic substrates, 95, 228

immunoassay, urease conjugates and electrode detection, 95, 483

assay for tetrodotoxin binding to detergent extracts of excitable tissues (Eiectrophorus eiec- tricus), 99, 72

Ellman’s reagent, see 5,5’-Dithiobis(2-nitrobenzoic acid)

Elongation factor Tu -GTP-aminoacyl-tRNA complex, quantitative as-

say for formation, 92, 123 Elution

positions, chromatography of sulfur amino acids on single-column amino acid analyzer, 98, 293

Elutriation apparatus, centrifugal, resolution of erythrocytes

(human), 98, 112 Embryos

immunosensors, amperometric determination of chorionic gonadotropin (human), 94, 22

intestine microsomal fractions prepared for studying drug metabolism (rat), 93, 73

levels, determination by immunoelectrophoresis (Neurospora crassa), 95, 97

marker assay, documentation of purity of sarcolemmal and sarcoplasmic reticulum fragments and mitochondria after isolation of myocardial subcellular organelles (canine), %, 293

mass, solid phase radioimmunoassay for lipoprotein lipase (chicken), 94, 346

microdetermination of polyamines with oxidase (snake venom), 92, 453

microdetermination of serum free fatty acids (human, rabbit), 98, 341

radiorespirometer for continuous monitoring of development (chicken), 98, 375

Endoglycosidases

modification in assay for inorganic phosphate, 95, 520

use for structural studies of glycoconjugates (review), 100, 1

Endoplasmic reticulum liberation of intracisternal A-type particles using

nitrogen cavitation (mouse), 97, 85 Enepyranuronic acid

nick-closing, fluorometric assay (simian), 93, 346 polyacrylamide gel electrophoresis, tannic acid

staining and extraction, 95, 575 -polymer film, application of photochemical reac-

tions of bis-azido compounds, 97, 320 proteolytic, assay by the fluorescence polarization

technique, 92, 222 residues for hyaluronate, quantitative assay of

hyaluronate, 96, 474 Enolase

activity, detection on polyacrylamide gels, 98, 226 Enzymes, see also specific enzymes

activity assays using permeabilized cells of Neurospora,

92, 356 automated fluorometric analysis (rat), 94, 166

-antibody conjugates, preparation methods and application for enzyme immunoassay, 100, 108

assay

renaturation, effect of composition of sodium dodecyl sulfate preparations after polyacrylamide gel electrophoresis, 100, 357

reversed-phase liquid chromatography, practical aspects applied to biochemical and biomedical research (review), 99, 1

size determination by radiation inactivation, 92, 2 stabilization by immobilization (review), 93, 1 transamidating, dansylcadaverine specific staining,

93, 453 transamidating, fluorescent activity staining pro-

cedure, 93,445 hypoxanthine-guanine phosphoribosyltransferase

in fibroblast (human), 92, 403 nature of random experimental error, 94, 265 optimizing coupled enzyme assays, 99, 142

auxiliary coupling, fluorimetry to assay ATPase activity (rabbit muscle), 92, 375

bacterial alkaline phosphatase, analysis on RPC-5 of oligoadenylates, 92, 276

trypsin-like substrate, synthesization of thiobenzyl benzloxycarbonyl-L-lypsinate, 93, 223

Epidermis growth factor, radioreceptor assay of tissue content

(human, rat), 93, 286

95, 433

tyrosinase, spectrophotometric determination of cresolase activity (Rana esculenta ridibunda),


Epinephrine radioenzymatic assay for dopamine and N-acetyl-

dopamine (gastropods), 96, 175 Epithelial cells

intestine microsomal fractions prepared for studying drug metabolism (rat), 93, 73

Epoxide hydrases activity, fluorometric assay, 97, 340 partition assay of activity, 92, 16

Erythrocytes chromatographic purification of glutathione reduc-

tase on 2’,5’-ADP-Sepharose and crystallization of enzyme (porcine), 98, 335

calorimetric determination of surface sialic acid residues, 97, 346

immature, preparation of undegraded polysomes and active mRNA (chicken), 98, 464

plasma membrane, reduced cholesterol/phospholipid molar ratios (human), 95, 344

purification glyoxalase I by affinity chromatography and

separation (human), 92, 390 S-2-hydroxyacylglutathione hydroiase (rat), 98,

472 quantification of glucose entry in infinite-& con-

ditions, 92, 361 rapid purification and direct spectrophotometric

assay of Saminolevulinic acid dehydratase (human), 97, 277

resolution by centrifugal elutriation (human), 98, 112

separation, nonsynchronous flowthrough coil planet centrifuge, 94, 249

separation of 1,2-diacylglycerol from triglycerides and phospholipids, 98, 123

o-Erythrose 4-phosphate enzymic method for analysis, 95, 250

Escherichia coli

filter paper assay for measurement of 4-thiouridine- tRNA sulfurtransferase activity, 98, 146

whole-cell assay for anthranilate synthetase, 96, 152 Esterproteases

identification and inhibition in submandibular gland (mouse), 93, 251

Esters cholesteryl, conditions for quantitative preparation

of methyl esters, 94, 402 lipids; rapid cleavage and large-scale isolation from

brain of cerebroside sulfate (porcine), 100, 364 methyl

derivative, chromatographic determination in rumen liquor, plasma, and tissue (ruminants), 99, 324

methanolysis of cholesteryl esters, conditions for quantitative preparation of, 94, 402

preparation with diazomethane for chromatographic analysis of abscisic acid (potato), 97, 331

octamethyl, chromatographic separation, 95, 444 peptidyl ethyl, merits of chromophoric and fluoro-

genie leaving groups in assay for serine proteases (human, porcine), 99, 53

phosphate, detection in thin layer chromatography, 9% 6

Estradiol immobilized antiserum, use in study of receptors

and estradiol-binding proteins, 94, 278 Estrogens

receptor, assay by thin-layer gel filtration (mouse), 94, 29

separation by high-pressure liquid chromatography, 92, 199

Ethanol precipitation, purification of RNA resolved by

analytical and preparative electrophoresis, 98, 358

Ethidium dimer, reagent for fluorimetric determination of

nucleic acids, 94, 259 Ethidium bromide

fluorometric measurement of DNA reassociative kinetics (rat), 94, 121

spectrophotometric determination of tetraphenylboron and tetraphenylarsonium, 92, 462

Ethionine metabolism, synthesis of radioactive 3-methylthio-

propionate and other alkylthio fatty acids, 98, 281

Ethyl acetate extraction, fluorescence assay for 4’-(9-acridinyl-

amino)methanesulfon-m-anisidide, 96, 504 Ethylene

liberation from I-aminocyclopropane-I-carboxylic acid, quantitative determination, 100, 140

Ethylenediaminetetraacetate (EDTA) -ferrous redox reaction with cytochrome c, com-

parison between stopped-flow kinetic and chronoamperometric kinetics (equine), 96, 326

Ethylenedinitrilotetraacetic acid conversion of amino acids to analogs, chelating

agents, 109, 152 Ethynylestradiol

analysis of birth control pills, mass-analyzed ion kinetic energy spectrometry, 96, 104

Etorphine receptor-binding assays, application of miniature

ultracentrifuge, 96, 395 Exchange chromatography

cation, analysis of polyamines, histamine, and I-methylhistamine, 94, 431

subunit, behavior of a-chymotrypsin, 95, 89 Exogludosidases

use for structural studies of glycoconjugates (review), 100, 1

Experimental error


random, nature when acetylcholine hydrolase and alcohol dehydrogenase are assayed, 94, 265

Exponential

microwell cultures, quantitative assay for collagen synthesis, 96, 220

single, fitting to biological data, nonparametric method described, 94, 270

Extrapallial fluid

quiescent and stimulated diploid lung, mitogenic and inhibitory effects of carrier ampholytes (human), 98, 27

chemical and spectral characterization (Myrilus edulis), 100, 324

radioassay of in vitro protein synthesis, 96, 139 skin, problems and solutions for detection of

arylsulfatase A activity after electrophoresis (human), 97, 36

F uptake of low density lipoproteins, 92, 74 Fibroin

Fast green properties as proteins stain and dye impurities

and effect on protein-dye binding with gels, 96, 262

Fatty acids

isolation of mRNA using proteinase K and sodium perchlorate, 98, 116

Fibronectin

alkylthio, synthesis based on degradation of labeled methionine, 98, 281

-mediated attachment of ovary cells to collagen substrates (Chinese hamster), 94, 308

Films

binding to immobilized albumin, chromatographic study (bovine), 99, 352

free, enzymatic microdetermination (human, rabbit), 98, 341


gelatin; containing lithium, rubidium, strontium, or copper, analyzed by secondary ion mass spectrometry, 92, 213

protein, 1H-2H exchange on, frustrated multiple internal reflection infrared spectroscopy, 94, 6

Filtration

long-chain, calorimetric determination in subcellular organelles, 97, 403

Feces

assay for measurement of 4-thiouridine-tRNA sulfurtransferase activity (Escherichia co/i), 98, 146

assay of glycogen synthase, 94, 409 gel

solubilization and assay in liquid scintillation counting (mouse, rat), 99, 288

Ferredoxins

peptide purification in aqueous pyridine-ammonia, 95, 397

hydrogenase-linked assay, 93, 216 liberation of labile sulfur by alkaline zinc reagent,

95,452 Ferricyanide

quantitation of peroxidase-antibody binding to placental membrane fragments (human, rabbit), 98, 53

glass fiber filters

effect on decarboxylation of [I-“Clpyruvate by pyruvate dehydrogenase (bovine), 98, 85

Ferritin hepatic, immunodiffusion method of measurement

reevaluated (rat), 98, 204 polycationic, measurement of anionic sites on

surfaces of kidney cells (hamster), 97, 400 Fibrinogen

staining of glycoproteins in polyacrylamide and agarose gels with fluorescent lectins, %, 208

Fibrinopeptides

assay of glycogen synthase, 94, 409 electrophoretic selection and fractionation of

poly(A)-containing RNA and poly(A), 92, 280 lipid-impregnated filters, study tool for electric

current-generating proteins, 96, 250 nitrocellulose filter binding

protein-nucleic acid, 93, 204 quantitation of histidyl-tRNA-ATP phosphori-

bosyltransferase complex, 92, 22 separation of small vesicles from suspension, %,

384

chromatographic assay (bovine), 96, 246 Fibroblasts

thin-layer gel, assay of estrogen receptor (mouse), 94, 29

Fingerprinting assay of hypoxanthine-guanine phosphoribosyltrans-

ferase (human), 92, 403 biochemical determination of tubulin-microtubule

equilibrium in cultured cells (human), 96, 155 carcinogen treated embryo, simultaneous prepara-

tion of nuclear DNA, RNA, and protein (hamster), 97, 410

gradient thin-layer chromatography of oligonucleotides on DEAE-cellulose as alternative to homochromatography, 98, 346

Flavin

cultured, application of neuraminidase, 95, 222 foreskin, isolation and purification of type I

procollagen by adsorption to glass beads (human), 96,433

adenine dinucleotide and analogs, purification from immobilized glucose oxidase and o-amino acid oxidase, 95, 156

Flavocytochromes c preparation of subunits (Chlorobium limicola f .

thiosulfalephi[um, Chromatium vinosum), 95, __- 209


Flavodoxin hydrogenase-linked assay, 93, 216

Flavoprotein preparation of subunits of flavocytochromes c

(Chlorobium limicola f. thiosulfatophilum, Chromatium vinosum), 95, 209

Fluorescamine micromethod for assay of primary amines and

proteins, reexamination of conditions of reaction, 99, 379

reactions with chromosomal proteins (herring, salmon), 99, 274

Fluorescence activity, staining procedure for transamidating en-

zymes, 93, 445 analysis of lifetime curves, 95, 32 assay for 4’-(9-acridinylamino)methanesulfon-m-ani-

sidide based on alkaline hydrolysis, 96, 504 assay for collagenase-like peptidase activity, 100,

43 assay for tyrosine hydroxylase activity based on

isolation of enzymatically formed dopa (brain), 93, 82

chelating agents, conversion of amino acids to analogs of ethylenedinitrilotetraacetic acid, loo, 152

dansylcadaverine staining for transamidating enzymes, 93,453

derivatives, o-phthalaldehyde reaction with peptides dependent upon free c-amino group lysine, 94, 371

determination of nucleic acids using ethidium dimer reagent, 94, 259

-immunoassay on lymphoblastoid cell suspensions (human), 94, 176

intramolecularly quenched fluorogenic substrates for hydrolytic enzymes, 95, 228

labeled cyanogen bromide cleavage products, gel electrophoresis, 95, 122

lectins, staining of glycoproteins in polyacrylamide and agarose gels, 96, 208

micromethod for assay of primary amines and proteins with fluorescamine reexamination of conditions of reaction, 99, 379

monitoring, characterization of proteins and peptides, 95, 117

quenching dansyl serum albumin, determination of total

and free serum bilirubin concentrations (bovine, human), 96, 364

rapid-mixing apparatus for continuous-flow studies, 97, 428

titration, graphical correction procedure for inner filter correction procedure for inner filter effect, 96,448

relative binding constants of biologically active, intercalating stains, electrophoretic determination, 95, 329

simultaneous measurement with phosphorescence using synchronously gated photon counters, 97, 158

spectroscopy, direct measurement of fluorescent probes associated with undisturbed monolayers of cultured fibroblasts, 92, 74

stopped-flow technique, measurement of dead time, 93,442

synchronously pumped krypton ion dye laser system, biochemical application, 97, 17

Fluorochromes merits of leaving groups in assay for serine pro-

teases, substrates for leukocyte and pancreatic elastase (human, porcine), 99, 53

Fluorography detection of radioactivity in polyacylamide gels

with water-soluble fluor, sodium salicylate, 98, 132

localization of peptides, 94, 150 Fluorometry

analysis of amino acid, effects of reaction temperature and thiol, 93, 174

analysis of polyamines, histamine and I-methylhistamine, 94, 431

apparatus amino acid analyzer, construction of instrument

using o-phthalaldehyde as detection agent, %, 298

titrimetric assay of glycosaminoglycans, 94, 378 assay

adenosine, inosine, and hypoxanthine, 95, 377 ATPase using auxiliary-coupling enzymes (rabbit

muscle), 92, 375 enzymatic determination of ammonia, 95, 507 y-glutamyl transferase, 100, 136 neuraminidase with sodium substrate, 94, 287 nick-closing enzyme (simian), 93, 346 pyridoxal and pyridoxal 5’-phosphate based on

reductive amination, 96, 426 quantitation of aminosugars, 95, 527 UDP-glucuronyltransferase using 3-hydroxy-

benzo(a)pyrene as substrate, 98, 394 urea in urine, plasma, and tubular fluid, 98,

136 automated analysis

amino acids, 94, 313 DNA, protein and enzyme activities (rat), 94,

166 continuous assay of epoxide hydrase activity, 97,

340 determination of trioses with o-phenylphenol, 94, 1 determination of tryptophan (plasma), 92, 338 front-face, optically dense liquid samples, 94, 15 measurement of diamine oxidase activity in amniotic

fluid (human), 96, 4t 1 measuring DNA reassociative kinetics (rat), 94,

121 microassay for determination of glutathione per-

3% CUMULATIVE SUBJECT INDEX

oxidase activity, application to blood platelets (human), 98, 154

monitoring enzymic hydrolysis of terminal galactose from GM,-ganglioside, 94, 186

partition of bilirubin among blood components, basis for rapid microassays of bilirubin and bilirubin binding capacity in blood (human), loo,25

proline reductase assay with o-phthalaldehyde, 95, 44

pyridine nucleotide-linked assays, measurements of glycolytic and tricarboxylic acid cycle metabolites, 97, 394

quantitation of amino sugars in picomole range, 98, 190

simultaneous determination of noradrenaline and 3- methoxy-4-hydroxyphenylethyleneglycol sulfate in discrete brain regions (rat), 97, 352

Fluorophotometry enzyme immunoassay of thyroid-stimulating hor-

mone (human), 96, 419 p-Fluorosulfonyl [“Clbenzoyl-5’-adenosine

labeled enzymes, isolation of peptides derived from adenine nucleotide binding sites, 95, 39

5-Fhiorouracil chromatographic determination in biological speci-

mens (mouse), 97, 232 Folates

electrochemical reduction, 97, 48 oxidative and reductive cleavage, critical appraisal,

93, 91 separation of pteroylpolyglutamates by polyacryl-

amide gel chromatography, 92, 501 Folin assay

removal of Triton X-100 from aqueous solution using Amberlite XAD-2, 92, 447

Formate periodate oxidation of carbohydrates, determined by

isotachophoretic analysis, 98, 417 W-Formyltetrahydrofolate

citrovorum factor, radiochemical assay, 94, 483 Fractionation

aminoacyl tRNA, 94, 48 electrophoretic selection of poly(A)-containing RNA

and poly(A), 92, 280 floating device for fractionation of density gradients

from top, 97, 72 histones to determine separation factors (cricket),

94, 220 preparative high-performance liquid partition chro-

matography of proteins (review), 98, 1 preparative, yeast nucleic acids, 95, 174 protein, folate-Sepharose column as affinity ab-

sorbent, 98, 184 subcellular, estimation of protein concentration, 94,

160 Fragmentography

CNBr reaction, protein fingerprinting, 97, 382

gas chromatography, simultaneous analysis of tryptophan, tryptamine, indole-3-acetic acid, serotonin, and 5-hydroxyindole-3-acetic acid (rat brain), 92, 233

quantitative determination of carbamazepine and carbamazepine-IO,1 I-epoxide in brain areas (rat), 93, 115

Freeze-dry reduction of static charge, 92, 128

Fructose-l ,6-bisphosphate high specific radioactivity, synthesis and purifica-

tion from radioactively labeled inorganic phosphate, 96, 500

Fructosyltransferase inhibition, overcome using Triton X-100 by means

of sodium dodecyl sulfate (Streptococcus murans), 97, 173

Ftorafur, see N,-(2’-TetrahydrofuryB-5-fluorouracil Furanacryloyl tripeptides

spectrophotometric assay for angiotensin converting enzymes, 95, 540

Fusidic acid purification of cytoplasm elongation factor 2 and

organellar elongation factor G (eukaryote), 99, 434

G

Galactosamine determination on amino acid analyzer, separation

of anomers of glucosamine and galactosamine, 96, 77

discrimination fromglucosamine in mixtures, 94,394 quantitation by fluorometric assay, 95, 527 quantitation of glycosaminoglycan hexosamine using

3-methyl-2-benzothiazolone hydrazone hydrochloride, 98, 478

Galactose terminal, fluorometric monitoring of enzymic hy-

drolysis, 94, 186 Galactose oxidase

tritiated-potassium borohydride, use for labeling pectic polysaccharides (grapefruit), 98, 429

Galactosyl P-linked residues, purification of polysaccharides

and glycoproteins containing, 92, 41 Galactosyltransferases

disk assay for determination of activity (human), 98,95

Gallstones determination of lithogenic index of bile, 188, 146

Galvinoxyl spin probe for detecting phospholipid phase transi-

tions, 94, 322 Gangliosides

fluorometric monitoring of enzymic hydrolysis of terminal galactose from, 94, 186

isolation from small amounts of tissue, 94, 335

Gas chromatography enzymatic method for measurement in biological -chemical ionization mass spectrometry samples, 95, 512

analysis of phenylethylamines in brain (rat), Gel electrophoresis 96, 308 agarose-urea, analytical and preparative resolution

estimation of sphingolipids with reference to of complex mixtures of RNA molecules, 98, ceramides of plasma (human), 97, 323 358

degradation steps of melittin, attachment of trypto- apparatus; preparative, large scale separations, phanyl peptides to 3-aminopropyi-glass suited loo, 304 for subsequent solid-phase degradation, 97, 56 chemical and spectral characterization of extra-

determination of hexamethylmelamine in plasma pallial fluid (Mytilus edulis), 100, 324 (mouse), 99, 441 determining relative binding constants of biologi-

determination of protein-bound dodecyl sulfate, cally active gels, intercalating fluorescent stains, 92, 444 95, 329

electron-capture, estimation of oxalic acid in urine fractionation of RNAs by partial denaturation with (canine), 94, 470 methylmercuric hydroxide, 99, 200

ethylene, assay for l-aminocyclopropane-l-carboxy- measurement of DNA length, 100, 319 lit acid, 100, 140 methyl mercuric hydroxide, size distribution of

-mass spectrometry labeled heterogeneous nuclear RNA and mRNA application to analysis of prostaglandin E, in (HeLa cell, mouse), 99, 146

biological media (rabbit), 98, 455 ribosomal proteins, tracking marker, 92, 203 characteristics and assay of tefe-methylhistamine, sodium dodecyl sulfate

96, 56 behavior with reference to SDS-protein com- detection of 5-methylcytosine and quantitation of plexes, 92, 243

5methylcytosine/cytosine ratio in DNA by, 94, -polyacrylamide gels, fluorescent labeled cyano- 297 gen bromide cleavage products, 95, 122

determination of cytokinins, 92, 111 -polyacryIamide gels, recovery of proteins, 94, selected ion recording, determination of nicotine 274

in biological fluids, 94, 4 11 -polyacrylamide gels stained with Coomassie micromethod analysis of amino acids, 92, 426 blue, extraction of proteins and peptides, 96, micromethod for determination of r5N enrichment 352

in plasma lysine, 94, 242 -polyacrylamide slab, tritium labeling of protein, quantitative analysis of 16,16-dimethyl-prostaglandin I 95, 359

ES. 100, 109 protein fingerprinting, 97, 382 quantitative determination of carbamazepine and radioiodination of protein mixtures, 95, 568

carbamazepine-IO, 1 I-epoxide in brain areas two-dimensional, visualization of proteins after (rat), 93, 115 isoelectric focusing (Smittia spec.), 98, 81

simultaneous analysis of substrates, products, and use of liquid polyacrylamide, 92, 99 inhibitors of xanthine oxidase, 98, 18 two-dimensional, identification of individual pro-

N-acetylation of amino sugar methyl glycosides, teins, 92, 174 94, 36 Gels, see also specific gels

analysis of trimethylamine in urine (human), 94, agarose 358 low gelling temperature, recovery of intact high

branched chain cy-keto acids, 92, 156 molecular weight RNA and DNA after electro- determination of 3-methylindoie in rumen liquor, phoretic separation, 98, 305

plasma, and tissue (ruminants), 99, 324 slabs, quantitative assays for trypsin inhibitors determination of cellulase activity (ovine), 92, 270 using immobilized trypsin, 98, 438 quantitation of sugars from differential culture cylindrical, longitudinal gel slicer, 97, 51

media, 99, 365 granulated, preparative flatbed electrofocusing with quantitative determination of ftorafur and 5-fluoro- natural pH gradients generated from simple

uracil in biological specimens (mouse), 97, buffers, 97, 95 232 isoelectric focusing, quantitative assay of protein

two-dimensional, electron-capture detection of kinase, 93, 52 guanethidine in plasma, 96, 118 porous silica, bioadsorbent and biocatalyst, 98,

Gaucher’s disease 238 distribution of glucocerebroside in liver (human), scanning, high resolution densitometer, 95, 520

95, 127, erratum, 98, 485 Genes GDP (guanosine 5’-diphosphate) overlap in densonucleosis virus, purification and



peptide mapping of solubilized proteins (Gal- leria mellonella), 99, 97

trans-Geranylgeranyl pyrophosphate Cl’-labeled, method for synthesis and purification,

92, 248 Ghosts

nuclear envelope, rapid high-yield isolation method ( feetrahymena pyriformis), 100, 282

Glands submandibular, methionine-o-naphtyl ester, ester-

protease substrate (mouse), 93, 251 Glass pores

controlled, derivatized, analysis of amino groups, 94, 60

Globin determination of ‘7-methylguanosine-containing 5’-

termini of mRNA (rabbit), 98, 102 y-Globulin

concentration of proteins using carboxymethyldextran (goat), 98, 353

Globulins testosterone-binding separation of androgen recep-

tor, 92, 164 Glucocerebroside

distribution in liver of patients with Gaucher’s disease, 95, 127, errarum, 98, 485

Glucosamine determination on amino acid analyzer, separation

of anomers of glucosamine and galactosamine, 96, 77

discrimination from galactosamine in mixtures, 94, 394

quantitation by fluorometric assay, 95, 527 Glucose

metabolism, separation of lactose from intermediate, 94, 340

permeation into erythrocytes, calculation of half- saturation concentrations, 92, 361

reducing moiety, specific maltose labeling (Spina- cacia oleracea), 96, 12

Glucose oxidase detection of glucose in amyloglucosidase hy-

drolysate (rat), 98, 47 immobilized, purification of flavin adenine dinucleo-

tide and its analogs, 95, 156 trehalose hydrolysis coupled to dioxide consump-

tion, 94, 360 Glucose 6phosphate dehydrogenase

DNA, protein and enzyme activity, fluorometric assay (rat), 94, 166

-glutathione reductase system, recycling analysis of NADP and NADPH, 99,297

P-Glucosidase immobilization, application of photochemical reac-

tions of bis-azido compounds to preparation of enzyme-polymer film, 97, 320

Glutamate

microfluorometric enzymatic assay for determination of ammonia, 95, 507

Glutamate decarboxylase unified assay for enzymes of glutamate metabo-

lism, 96, 24 Glutamate dehydrogenase

assayed by coupling to glutamate decarboxylase and measuring WO, release (Escherichia co/i), %, 24


Glutamic acid y-semialdehyde enzymic synthesis, isolation and characterization

of 2,4-dinitrophenylhydrazones, 96, 94 Glutaminase

assayed by coupling to glutamate decarboxylase and measuring ‘*CO2 release (Escherichia coli), w; 24

Glutamine synthetase activity, radioisotopic assay in tissue

extracts (mouse), 94, 82 Glutamine synthetase

enzymic procedures for determining average state of adenylylation (Escherichia co/i), 95, 275

y-Glutamyl transferase fluorimetric assay, 100, 136

Glutathione -bromosulfophthalein conjugate covalently linked

to Sepharose 4B, affinity chromatography of albumin (rat plasma), 92, 290

Glutathione peroxidase fluorimetric microassay for determination of ac-

tivity, application to blood platelets (human), 98, 154

Glutathione reductase chromatographic purification from erythrocytes on

2’,5’-ADP-Sepharose and crystallization of enzyme (porcine), 98, 335

-glucose 6-phosphate dehydrogenase system, recycling analysis of NADP and NADPH, 99, 297

GlutathioneS-transferase temperature dependence of activity, median method

for fitting single exponential to biological data, 94, 270

Glycamines analysis of neutral sugars using amino acid analyzer,

97, 166 y-Glycidoxypropyltriethoxysilane

trypsin purification, porous silica gel as bioadsorbent and biocatalyst, 98, 238

Glycoconjugates structural studies, use of endo- and exoglycosidases

(review), 100, 1 Glycogen

one-step enzymic assay of total carbohydrate content of liver homogenate (rat), 98, 47

metabolism, unified assay for enzymes, 96, 24 synthase, filtration assay, 94, 409


Glycogen synthase washing glycogen free of unreacted UDP-[WI-

glucoase by ascending chromatography, 100, 371

Glycolytic acid metabolites, measurement in sample aliquots with

tricarboxylic acid, 97, 394 Glycopeptides

actions of endo-P-N-acetylglucosaminidases (review), 100, I

analysis as borate complexes electrophoretically, 97, 438

Glycoproteins N-acetylation of methyl glycosaminide, 94, 36 -derived oligosaccharides, separation of by thin-

layer chromatography, 93, 167 detection in polyacrylamide gel with thymol and

sulfuric acid, 99, 474 factors affecting binding to concanavalin A and

agglutinin and the dissociation of their complexes (Ricinus communis), 99, 254

Ruorometric assay for quantitation of aminosugars, 95, 527

-lectin complexes, quantitation and differentiation by laser nephelometry, 96, 391

purification on high-capacity lectin affinity column (wheat germ), 97, 423

staining in polyacrylamide and agarose gels with fluorescent lectins, 96, 208

tumor virus, purification and characterization (mouse), 94,40

Glycosaminoglycans complete electrophoretic separation in monodimen-

sional run (animal), 99, 31 I electrophoretic separation (animal), 93, 37 fluorimeter for titrimetric assay, 94, 378 hexosamine, quantitation using 3-methyl-2-benzo-

thiazolone hydrazone hydrochloride, 98, 478 quantitation and interaction with Alcian blue in

dimethyl sulfoxide solutions, 93, 60 spectrophotometric estimation of concentrations,

99, 183 Glycosidases

structural information, analysis of glycopeptides as borate complexes by electrophoresis, 97, 438

structural study of glycoconjugates, use of endo- and exoglycosidases (review), 100, 1

Glyoxalase I purification from erythrocytes by affinity chro-

matography and separation (human), 92, 390 Glyoxalase II, see S-2-Hydroxyacylglutathione hy-

drolase cGMP (guanosine-3’:5’-monophosphate)

phosphodiesterase measurement, 100, 244 Gonadotropin

chorionic, amperometric determination (human), 94, 22

Gradients

cesium chloride function, initial density related to equilibrium

density, 94, 193 purification and characterization of DNA using

vertical rotor, 97, 196 continuous slabs, casting of without special equip-

ment, 92, 91 linear polyacrylamide gels, estimation of protein

molecular weights, 98, 160 low-cost programmer, 98, 142 natural pH, preparative flatbed electrofocusing in

granulated gels, 97, 95 voltage, electrophoretic mobility of high molecular

weight RNA (HeLa cell, mouse), 99, 146 Growth

calorimetric assay of hormone- and metabolite- induced changes in steady-state heat production by tissue (Zea mays), 100, 77

GTP (guanosine 5’-triphosphate) -elongation factor Tu-aminoacyl-tRNA complex,

quantitative assay for formation, 92, 123 enzymatic method for measurement in biological

samples, 95, 512 [a-s*P]GTP

synthesis in a single reaction vessel, 93, 124 Guanethidine

chromatographic electron-capture detection in plasma, 96, 118

Guanidine hydrochloride chromatographic estimation of molecular weights

of protein polypeptide chains, 97, 65 Guanidoacetate methyltransferase

radioactive assay, 95, 87 Guanosine triphosphate cyclohydrolase 1

purification on dihydrofolate-Sepharose affinity column, 100, 122

Guanylyl cyclase activity, modified ‘adenylyl assay in subcellular

tissue fractions, 93, 124

H

Halobacterium halobium electrophoretic mobility on a sucrose gradient,

92, 228 Haptens

rapid technique for semiquantitative mass screening by autoradiographic immunoassay, 92, 147

Haptoglobin concentration, measurement in plasma and other

fluids by spectrometry (human), 99, 421 radioassay based on binding to solid-phase hemo-

globin (rabbit), 93, 355 Hatching enzyme

assay using radioiodinated protein substrates (Xeno- pus laevis), 100, 352

Heart bicarbonate tissue measurement (rat), 95, 183


cardiac sarcoplasmic reticulum isolation (bovine), 99, 41

isolation of myocardial subcellular organelles by sucrose gradient ultracentrifugation (canine), 96, 293

mitochondria, chromatographic purification of malic enzyme (bovine), 100, 299

myocardial creatine phosphokinase and protein, effect of sodium desoxycholate on extraction (rat), 98, 278

myocardium, creatine phosphokinase and protein; effect of sodium desoxycholate on extraction (rat), 98, 278

Helium plasma, low-pressure microwave-induced emission

spectrometry, 95, 8 Hemoglobin

fetal, chromatographic separation of y cyanogen bromide peptides (human), 96, 113

quantification in polyacrylamide gels with benzidine, 94,211

quantitative determination using 3,3’,5,5’-tetramethylbenzidine dihydrochloride, 98, 388

Sepharose-bound, haptoglobin radioassay based on binding to (rabbit), 93, 355

spectrophotometric determination of myoglobin in cardiac and skeletal muscle, 92, 473

Heparin potentiometric titration determination of content

in heparin-Sepharose, 95, 449 Heptane

chloroform:methanol, calorimetric determination in subcellular organelles, 97, 403

Hexamethylmelamine chromatographic determination in plasma (mouse),

99,441 Hexokinase

enzymatic coupling system with NAD-pyrophosphorylase, NAD+ analysis, 97, 309

Hexosamines determination, tetraborate concentration on Mor-

gan-Elson color reaction, 98, 433 fluorometric assay for quantitation of aminosugars,

95, 527 fluorometric quantitation of amino sugars in pico-

mole range, 98, 190 glycosaminoglycans, quantitation using 3-methyl-

2-benzothiazolone hydrazone hydrochloride, 98, 478


High-performance liquid chromatography analysis

abscisic acid in tubers (potato), 97, 33 1 aflatoxins, 93, 409 histidine-containing dipeptides, polyamines, and

ammo acids (guinea pig), 93, 423 methylated purines in DNA treated with chemi-

cal carcinogens (rat), 108, 58

7-methylguanine in DNA (bovine, Escherichin coli), 97, 203

phenylthiohydantoin amino acids on DuPont Zorbax cyanopropylsilane columns, 108, 335

thiamine and its phosphate esters after conversion to highly fluorescent thiochrome derivatives, 97, 191

tissue nucleotides, 93, 227 analyzer and monitor for rapid microscale pep-

tide separations, 98, 178 anion-exchange, determination of 7-methylguano-

sine-containing 5’-termini of mRNA by NaB- [3H]4 labeling, 98, 102

application to analysis of prostaglandin E, in biological media (human, rat, rabbit), 98, 455

assay for deoxyribonucleoside triphosphates in cell extracts, 99, 268

assay of cetechol O-methyltransferase by determination of m- and p-U-methylated products, 98,452

assay of fibrinopeptides (bovine), 96, 246 characterization of proteins and peptides, 95, 117 determination of CoASH and application in assay

of long-chain acyl-CoA derivatives, 96, 181 determination of cyclooxygenase products and

prostaglandin metabolites, 93, 339 determinations of disaccharides resulting from enzy-

matic degradation of chrondroitin sulfates, 94, 231

determination of doxorubicin and its fluorescent metabolites, 94, 112

demonstration of endogenous retinoic acid in normal physiological conditions, compound of blood (human), 98, 402

determination of 5-hydroxytryptamine metabolites in isolated perfused lung (rabbit), 93, 143

estimation of molecular weights of protein polypeptide chains using guanidine hydrochloride, 97, 65

gradient programmer for linear gradient, 98, 142 identification and characterization of syn- and anti-

isomers of retinaloximes, 99, 304 isocratic resolution of amino acid thiohydantoins,

95,494 molecular weight separation of proteins and pep-

tides, 97, 176 preparation of tritium-labeled chloramphenicol and

its analogs, 96, 215 preparative partition of proteins (review), 98, 1 protein, multiple forms of lipoxygenase-1 (soybean),

95, 371 quantitative determination of acetanilide 4-hydroxy-

lase activity (mouse), 96, 201 quantitative determination of N-arylaceto- and N-

arylglycolhydroxamic acid in biochemical reaction mixtures, 98, 169

radioimmunoassays for vinblastine and vincristine (Vinca rosea), 95, 214

reversed-phase


analysis of nanogram levels of free amino acids, 92, 170

determination of free and conjugated bile acids as ultraviolet-absorbing ion pairs, 100, 260

practical aspects applied to biochemical and biomedical research (review), 99, 1

resolution of benzo[a]pyrene metabolites using organic amines and application to marine metabolites (Stenofomus versicolor), 99, 129

separation of acetylated oligosaccharides, 97, 184 separation of cinnimic acids and related com-

pounds, 93, 233 separation of diethylstilbestrol metabolites and

analogs, 92, 199 separation of DNA bases and deoxyribonucleo-

sides, 94, 237 separation of estrogens, 92, 199 separation of y cyanogen bromide peptides of

fetal hemoglobin (human), 96, 113 separation of a-keto acids as their 2,4-dinitro-

phenylhydrazine derivatives, 92, 3 1 separation of uroporphyrin I and III octamethyl

esters, 95, 444 simultaneous analysis of substrates, products, and

inhibitors of xanthine oxidase, 98, 18 voltammetric assay for aromatic L-amino acid

decarboxylase activity, 100, 160 Histamine

enzymatic isotopic assay, measurement of, in plasma and serum (rat), 94, 425

fluorometric analysis, 94,43 1 Histidine

biosynthesis, regulation, 92, 22 Histidinol dehydrogenase

immunodiffusion activity stain, 92, 189 Histidyl-tRNA

-ATP phosphoribosyltransferase complex, nitrocellulose filter binding, 92, 22

Histochemistry enzyme substrate, quantitative assays for trypsin

inhibitors using immobilized trypsin, 98, 438 Histones

electrophoretic analysis of basic proteins using phosphatidyl choline/urea solubilization, 100, 289

H3, dithiobis-(nitrobenzoic acid) induced inter- and intramolecular crosslinks in, 94, 465

separation factors, chromatographic fractionation (cricket), 94, 220

two-dimensional analysis, application of protein separation by molecular weight, 98, 410

Homocysteic acid determination of thioether-Iinked cysteine resi-

dues, 92, 489 Homogenates

cellular, nanogram assay of DNA, 92, 497 ion-exchange assay of sucrose synthetase and su-

crose phosphate synthetase (plant), 93, 196 Homooligonucleotides

dG, fractionation using RPC-5 column chromatography at high pH, 99, 213

Hormones -induced changes in steady-state heat production

by tissue, calorimetric assay (Zea mays), loo, 77

red pigment-concentrating iodination and purification, 92, 46

Horseradish peroxidase fluorophotometric enzyme immunoassay of thyroid-

stimulating hormone (human), 96, 419 immunoassay of chenodeoxycholate conjugates, 93,

361 Hyaluronate

determination by means of periodate-thiobarbituric acid assay, 96, 474

Hyaluronate lyase periodate-thiobarbituric acid assay, determination

of hyaluronate, 96, 474 Hybridization

detecting foreign DNA in plasmids (Escherichia co/i), 98, 60

labeled DNA, RNA transferred from agarose-urea gels on diazobenzyloxymethyl paper, 98, 358

quantitation of replicating polyoma DNA sequences (Haemophilus parainjluenzae), 96, 378

Hydrocarbons carcinogenic polycyclic, analysis of metabolism by

position-sensing proportional counting of thin- layer chromatograms, 99, 16.5

Hydrochloric acid detection of phosphate esters in thin layer chro-

matography, 96, 6 Hydrogen

-deuterium exchange by ultraviolet spectrophotometry, 92, 517

Hydrogen peroxide enzyme stabilization by immobilization (review),

93, 1 Hydrogenase

assay for ferredoxin and flavodoxin, 93, 216 Hydrolysis

alkaline analysis of y-carboxyglutamic acid, 92, 466 fluorescence assay of 4’-(9-acridinylamino)meth-

anesulfon-m-anisidide, 96, 504 enzymic, terminal galactose from GM,-ganglioside,

fluorometric monitoring, 94, 186 peptidyl derivative of [3H]aniline as sensitive,

stable, protease substrate, 97, 269 substrate by renin, effects of pH (human, por-

cine), 94, 125 selective, cholesteryl esters, quantitative prepara-

tion of methyl esters, 94, 402 Hydroperoxides

lipid, spectrophotometric determination, 99, 249 Hydroquinone

analysis of cY-tocopherol in liver employing direct

CUMULATIVE SUBJECT INDEX

attack on tissue with alcoholic alkali (ovine), 97,264

S-2-Hydroxyacylghuathione hydrolase puritication from erythrocytes (rat), 98, 472

p-Hydroxy-L-aspartate diastereoisomers, preparation of from cysteine sul-

finate and dihydroxyfumarate with heart aspartate aminotransferase (porcine), 93, 134

3-Hydroxybenzo(a)pyrene substrate for fluorometric assay for UDPglucuronyl-

transferase, 98, 394 3-Hydroxybutyrate

determination of radioactivity, 92, 390 9-(2-Hydroxyethoxymethyl)guanine

antiviral agent BW248U, radioimmunoassay, 98, 319

SHydroxyindole-3-acetic acid gas chromatography-mass fragmentography analysis

(rat brain), 92, 233 Hydroxylamine

derivatization of retinals, identification and characterization of syn- and anti-isomers of retinaloximes, 99, 304

Hydroxylapatite batch assay for quantitation of cellular DNA

damage (mouse, human), 97, 77 granulated

isolation of maltopentaose by chromatography (corn syrup), 92, 351

used in isolation of o-amino acid oxidase (porcine), 94, 105

Hydroxylation separation of acetanilide and its metabolites

(mouse), 96, 201 3-Hydroxy-3-methylglutaryl-coenzyme A

suppression by low-density lipoproteins, 92, 74 p-Hydroxyphenyl propionic acid

N-hydroxysuccinimide ester, iodination of collagens to high specific activity for use in radioimmunoassay, 96, 489

Hydroxyproline automated fluorimetric analysis, 94, 313

3-Hydroxysteroid dehydrogenase enzymatic method for quantitative determination

of 3-keto bile acids (Neurospora crassa, Pseudomonas testosteroni), 99, 408

preparation (Pseudomonas), 95, 286 S-Hydroxytryptamine

metabolism, determination of in isolated perfused lung by high-pressure liquid chromatography (rabbit), 93, 143

5-Hydroxytryptophol determination of in isolated perfused lung by

high-pressure liquid chromatography (rabbit), 93, 143

25-Hydroxyvitamin D high specific activity, radiochemical synthesis, 96,

481

Hyperbilirubinemia fluorometric study of partition of bilirubin basis

for microassays of bilirubin and bilirubin binding capacity in blood (human), 100, 25

Hypoxanthine enzymic conversion of inosine and adenosine,

fluorimetric assay, 95, 377 chromatographic simultaneous analysis with xan-

thine, allopurinol, oxipurinol, and uric acid, 98, 18

-quanine phosphoribosyltransferase, assay in human fibroblast lysates, 92, 403

Immobilization determination of enzyme structures, applied to sys-

tem urease/aluminium hydroxide, 100, 54 enzymes for stabilization (review), 93, 1

Immune complexes adsorbent used for protein synthesis assay in cells

and cell-free translations (Staphylococcus aureus), 97, 24

Immunoabsorption combined with isoelectric focusing and enzymatic

characterization in same gel, barley and malt amylases (rabbit), 93, 261

Immunoassay application of preparation methods for enzyme-

antibody conjugates, 100, 108 chemiluminescence enzyme, cortisol using peroxi-

dase as label, 97, 248 competitive-binding, luminol-assisted, of immuno-

globulin G (human), 93, 267 enzymic

chenodeoxycholate conjugates, 93, 361 thyroid-stimulating hormone (human), 96, 419 urease conjugates and electrode detection, 95,483

leucovorin, use of *251-labeled protein A to detect immunological binding, 95, 472

solid-phase, for immunoglobulin E, use of iZ51- labeled protein A (human), 93, 207

Immunodiffusion measurement of liver fenitin reevaluated (rat),

98, 204 two-cross technique, diffusion coefficients and pre-

cipitating titers of immunoglobulin (IgG) in serum antibodies, 93, 103

Immunoelectrophoresis curve fitting, 100, 51 determination of nitrate reductase (Neurosporu

crassa), 95, 97 fluorescent activity staining procedure for trans-

amidating enzymes, 93, 445 Immunofluorescence

quantitative assay on lymphoblastoid cell suspensions (human), 94, 176

Immunogiobulins


analysis of glycopeptides as borate complexes by electrophoresis, 97, 438

I@ solid-phase immunoassay using ‘*Wabeled pro-

tein A as a tracer (human), 93, 207 w

immunoabsorption and isoelectric focusing of amylases (rabbit), 92, 261

luminol-assisted, competitive-binding immunoassay (human), 93, 267

sized aggregates, spectrophotometric assay with turbidity correction, 99, 346

two-cross immunodiffusion for determination of precipitating titers and diffusion coefficients in body fluids, 93, 103

radioiodinated, mdioimmunological screening method for specific membrane proteins (Escherichiu

co&), 97, 153

lmmunoreaction effect of detergents on antibody-antigen interac-

tion, 98, 445 Immunosensors

enzyme, amperometric determination of chorionic gonadotropin (human), 94, 22

Indole chromatographic determination in rumen liquor,

plasma, and tissue (ruminants), 99, 324 Indole-3-acetic acid

gas chromatography-mass fragmentography analysis (rat brain), 92, 233

Infrared analysis, extraction and measurement of deuterium

oxide at tracer levels, 98, 208 Infrared spectroscopy

frustrated multiple internal reflection, lH-ZH exchange on protein films, 94, 6

measurement of expired 13C02 (rat), 96, 64 Inosine

enzymic conversion to hypoxanthine, fluorimetric assay, 95, 377

determination of electrophoretic mobility of macromolecules (bovine), 96, 355

Interferons acid ethanol-extracted lymphoid, purification by

Blue Sepharose chromatography (human), 98, 214

Intestine microsomal fractions prepared for studying drug

metabolism (rat), 93, 73 mucosa, steroid receptor assay for quantitation of

la,25dihydroxyvitamin Da-receptor complexes (chicken), 92, 314

[3H]Inulin improved microdialysis technique, 94, 109

Iodate periodate oxidation of carbohydrates, determined

by isotachophoretic analysis, 98, 417

Iodination electrolytic, preparation of bovine parathyroid

hormone, 92, 67 peptide hormones containing tryptophan, 92,46

Iodine monochloride preferential radioiodination of small amounts of

protein in presence of excess lipids, 99, 92 Iodothyronine

separation from thyroid hormones by thin-layer and exchange chromatography, 93, 3 1

Ion exchange chromatography analysis of urinary 3-methylhistidine (human),

93, 394 examination of sulfur amino acids on single-column

amino acid analyzer, 98, 293 separation of thyroid hormones and their metabolites

(simian), 93, 3 1 sucrose synthetase and sucrose phosphate syn-

thetase in plant homogenates, 93, 196 Ion monitoring

experiments in determination of cytokinin, 92, 111 multiple specific, detection of 5methylcytosine in

DNA, 94, 297 selected, determination of nicotine in biological

fluids at picogram levels, 94, 411 selective, micromethod analysis of amino acids us-

ing gas chromatography and mass spectrometry, 92, 426

Ionophores A23 187, incorporation in lipid bilayer of unilamellar

vesicles, 94, 302 Iron

catalytic method for determination of trace amounts in biological material, 99, 28

-EDTA redox reactions with cytochrome c, comparison between stopped-flow kinetics and chronoamperometric kinetics (equine), 96, 326

ion, interference in spectrophotometric determination of ascorbic acid eliminated, 96, 1

-sulfur center, liberation of labile sulfur from ferredoxins by alkaline zinc reagent, 95, 452

Isoamyl alcohol single extraction removal of Triton X-100, 93, 139

Isocitrate dehydrogenase NAD+-linked, calcium concentration in presence of

magnesium and chelating effecters, 95, 62 Isoelectric focusing

combined with immunoabsorption and enzymatic characterization in same gel, barley and malt amyiases (rabbit), 93, 261

concentration and two-dimensional electrophoretic analysis (human), 95, 48

detection of enolase activity on polyacrylamide gels, 98, 226

gels, quantitative assay of protein kinase, 93, 52 mitogenic and inhibitory effects of carrier ampho-

lytes on quiescent and stimulated diploid lung fibroblasts (human), 98, 27


point standardization, in two-dimensional electrophoretic technique, 93, 3 12

preparation of EE isozyme of liver alcohol dehydrogenase (equine), 99, 392

second dimension separation of cellular proteins, 93, 329

quantification by gas-liquid chromatography, 92, 156

a-Keto-/3-methylvaleric acid quantification by gas-iiquid chromatography,

92, 156 Ketone bodies

study of plasma membrane actin (chick fibroblasts), 95, 236

two-dimensional electrophoresis of small-molecular- weight proteins, 99, 33

visualization of proteins during two-dimensional gel electrophoresis (Smittia spec.), 98, 81

Isotachophoresis

direct determination of radioactivity in carboxyl group and acetone moiety, 92, 390

a-Ketovaleric acid quantification by gas-liquid chromatography,

92, 1.56 Kidney

amino acid spacing, on polyacrylamide gel, 94, 202 periodate oxidation analysis of carbohydrate, 98,417

Isotopes

decarboxylation of [I-Wlpyruvate by pyruvate dehydrogenase, effect of fenicyanide (bovine), 98, 85

multiple, radioautography using color negative films, 93, 189

fluorometric assay for urea in urine plasma, and tubular fluid, 98, 136

stable dilution, determination of urinary oxalic acid and oxalate pool size, 94, 477

Isotopic exchange 32P1 incorporated into -y-position of ATP, 93, 272

Isozymes hepatic alcohol dehydrogenase, preparation of EE

isozyme (equine), 99, 392 homodimeric creatine kinase, purification with blue

Sepharose CL-6B (Lepomis cyanellus), 94, 89 purification of glyoxalase I from human erythrocytes

using affinity chromatography and separation, 92, 390

Isopropanol

isolation of o-amino acid oxidase (porcine), 94, 105 papillary slices, analysis of prostaglandin E, in

biological media (human, rabbit, rat), 98, 455 radiolabeled polycationic ferritin, measurement of

anionic sites on surfaces (hamster), 97, 408 source of histamine-N-methyl transferase for enzy-

matic assay of histamine (rat), 94, 425 Krypton

ion dye laser fluorescence system, biochemical ap- plications, 97, 17

L

Labeling precipitation, isolation of RNA, 98, 116

J

Juvenile hormone I metabolism, 92, 16

Juvenoid

base composition of nanogram quantities of un- labeled nucleic acids, 93, 26

fluorescent, of high molecular weight RNA, 93, 399 RNA sequence determination (rabbit), 93, 321

P-Lactam

metabolism of expoxide-containing, 92, 16

K

antibiotics, estimation, 95, 413 Lactate dehydrogenase

calculated molecular weight of proteins in high ionic strengths, contribution of apparent isopotential specific volume (Homarus americanus), 98, 481

a-Keto acids branched chain, gas-liquid chromatography,

92, 156

determination in blood serum, peroxidase-catalyzed oxidation of NADH as indicator reaction (human), 97, 255

p-Lactoglobulin B derivatives, resolution of syn- and anri-isomers,

92, 31 separation by high-pressure liquid chromatography,

92, 31 3-Keto acids

estimation of apparent diffusion coefficient, continuous optical scanning in polyacrylamide gel electrophoresis, 97, 120

Lactones

bile, enzymatic method for quantitative determination (Neurospora crassa, Pseudomonas testosteroni), 99, 408

cy-Ketoglutarate

derivatives and coupling to solid support, attachment of tryptophanyl peptides to 3-aminopropyl-glass , 97, 56

Lactoperoxidase


a-Ketoisocaproic acid

iodination, radioimmunoassay of vasoactive intestinal polypeptide (porcine), 97, 286

radioiodination of protein mixtures for electrophoretic analysis, 95, 568


Lactose synthesis and secretion, detection and quantitation,

94, 340, erratum, 98, 485 Lectins

N-acetyl-D-glucosamine, model system for study of binding specificity, 99, 221

cell-adhesion studies, affinity materials for purification (peanut), 95, 260

factors affecting binding of glycoproteins (Ricinus communis), 99, 254

fluorescent, staining of glycoproteins in polyacrylamide and agarose gels, 96, 208

-glycoprotein complexes, quantitation and differentiation by laser nephelometry, 96, 391

high-capacity affinity column, glycoprotein purification (wheat germ), 97, 423

purification by affinity chromatography on a N, N’, N”-triacetylchitotriose-Sepharose matrix (potato), 93, 88

S-Lactoyi-glutathione preparation methodology, 98, 472

Leghemoglobin oxygenated, sources of free dissolved O2 at low

concentrations (Rhizobium), 96, 165 spectrofluorometric assay, 92, 11

Leucine aminopeptidase protease activity measurement by means of L-amino

acid oxidase-peroxidase system (Aspergillus japonica), 97, 11

Leucovorin immunoassay for vinblastine and vincristine (Vinca

rosea), 95, 214 immunoassay, use of ‘251-labeled protein A to detect

immunological binding, 95, 472 Leukocytes

elastase, sensitive substrates: study of merits of various chromophoric and fluorogenic leaving groups in assay for serine proteases (human), 99, 53

interferons, purification by chromatography (human), 98, 214

Ligands absorbing, fluorescence quenching titration limita-

tion from inner filter effects, 96, 448 application of S-(6-aminohexyl)-amino-GTP and

-GDP in affinity chromatography, 99, 189 binding parameters, chromatographic purification of

neurophysins on methionyl-tyrosyl-phenylalanyl-aminohexyl-agarose, 97, 302

binding of fatty acids and drugs to immobilized serum albumin, chromatographic study (bovine), 99, 352

equilibrium binding constants, measurement of macromolecule (review), 97, 1

-protein interactions, nitrocellulose filter binding in quantitation, 92, 22

quantitation of sialoglycoconjugate immobilization on insoluble supports, 100, 221

thioglycoside, preparation of polyacrylamide gels containing, 95, 260

Light emission from crude and purified luciferase, fac-

tors affecting kinetics (firefly), 95, 194 quasielastic scattering studies of reacting systems,

mixing device, 99, 217 scattered from excitation lamp, fractional contribu-

tion to normalized raw fluorescence lifetime curve, 95, 32

Linoleic acid hydroperoxide assay for lipid peroxides in tissue (rat), 95, 351

Lipids ester; rapid cleavage and large-scale isolation from

brain of cerebroside sulfate (porcine), 100, 364 excess presence, preferential radioiodination of

small amounts of protein, 99, 92 hydroperoxides, spectrophotometric determination,

99, 249 -impregnated filters as tool for studying electric

current-generating proteins, 96, 250 interfering effect on Lowry method protein assay

(review), 108, 201 peroxides, assay in tissues by thiobarbituric acid

reaction (rat), 95, 351 quantitative analysis, diacylglycerol kinase to deter-

mine 1,2-diacylglycerol levels (Escherichia co/i), 98, 123

separation of gangliosides by thin-layer chromatography, 94, 335

vesicles containing high levels of entrapped radioactive cations, preparation, 94, 302

lipase, solid phase radioimmunoassay (chicken), 94, 346

low-density labeled, uptake by normal human fibroblasts, 92, 74

Liposomes, see Phospholipids Lipoxygenase

instrumental improvements for rapid, detailed kinetic studies (soybean), 96, 341

test paper for detection, 99, 118 Lipoxygenase-1

multiplicity, chromatographic studies on protein (soybean), 95, 371

Liquid chromatography, see also High-performance liquid chromatography reversed-phase ion-pair, analysis and purification of

acyl coenzyme A thioesters, 94, 417 reversed-phase, practical aspects applied to bio-

chemical and biomedical research (review), 99, 1 Liquid scintillation counting

one-step method in 3H- and Y-containing gel slices, 99, 161

solubilization and assay of blood and feces (mouse, rat), 99, 288

Lithium gelatin films analyzed by secondary ion mass spec-

trometry, 92, 213


Liver alcohol dehydrogenase, preparation of EE isozyme

(equine), 99, 392 analysis of o-tocopherol employing direct attack on

tissue with alcoholic alkali in presence of hydroquinone and pyrogahol (ovine), 97, 264

bicarbonate tissue measurements (rat), 95, 183 chromatographic determination of 7-methylguanine

in DNA (rat), 97, 203 chromatographic resolution of benzo[a]pyrene

metabolites using organic amines and application to marine metabolites (Stenoromus versicolor), 99, 129

chromatographic separation of mannosylretinylphosphate from dolichylmannosylphosphate (rat), 97, 298

determination of phosphoenolpyruvate carboxykinase activity with deoxyguanosine S-diphosphate as nucleotide substrate (guinea pig), 96, 279

enzymatic preparation of 3’-phosphoadenosine- 5’-phosphosulfate (rat), 96, 34

Gaucher’s disease, distribution of ghrcocerebroside (human), 95, 127, errurum, 98, 485

homogenates arginase assay (bovine), 95, 479 specific determination of total carbohydrate con-

tent in single step using purified amyloglucosidase (rat), 98, 47

immunoditfusion method of ferritin measurement reevaluated (rat), 98, 204

isolation of intact mitochondria (rat), 93, 200 metallothionein, radioimmunoassay with labeled

zinc thionein (rat), 98, 89 microsomes, separation of acetanilide and its

hydroxylated metabolites and determination of acetanilide 4-hydroxylase activity (mouse), 96, 201

mitochondrial adenylate kinase, measurement of mitochondrial oxidative phosphorylation (rat), 9% 7

photometric assay for alcohol dehydrogenase activity in blood serum (equine, human), 99, 65

properties of tyrosine aminotransferase (rat), 95,188 purification of regulator of cyclic nucleotide phos-

phodiesterase utilizing calcium-binding ability (rat), 96, 407

spectrophotometric cycling assay for reduced coenzyme A and its esters (mouse), 97, 103

sterol epoxide hydrase activity from (mouse), 94, 383

visualization and quantification of total complement of free and membrane bound ribosomes (rat), 92, 305

Livetin chromatographic separation from egg yolk (chicken),

94.53

Lowry method protein assay comparison with dye binding method, 94, 160 Fohn phenol protein quantitation (review), 180, 201 interference by detergents, 96, 21 modified for presence of Triton X-100, 93, 139

Luciferase factors affecting kinetics of light emission (fire-

fly), 95, 194 Luminol

-assisted, competitive-binding immunoassay of immunoglobulin G (human), 93, 267

chemiluminescence enzyme immunoassay of cortisol using peroxidase as label, 97, 248

Lung quiescent and stimulated diploid fibroblasts, mito-

genie and inhibitory effects of carrier ampholytes (human), 98, 27

Lymphoblastoids cell suspensions, immunotluorescence assay for anti-

bodies and surface antigens (human), 94, 176 interferons, chromatographic purification (human),

98, 214 Lymphocytes

radioassay of in vitro protein synthesis by fibroblasts, 96, 139

tonsil, reduced cholesterohphospholipid molar ratios (human), 95, 344

Lymphoids acid ethanol-extracted interferons, purification by

Blue Sepharose chromatography (human), 98, 214

Lysine free e-amino group, o-phthalaldehyde reaction with

peptides to yield fluorescent derivatives dependent upon, 94, 371

plasma, micromethod for determination of 15N enrichment, 94, 242

Lysine vasopressin chromatographic purification of neurophysins on

methionyl - tyrosyl - phenylalanyl - aminohexyl-agarose and measurement of hgand binding parameters, 97, 302

Lysosomes contamination, cardiac sarcoplasmic reticulum isola-

tion from heart (bovine), 99, 41 Lysozymes

analysis of recombinant colonies, detecting foreign DNA in plasmids (Escherichia coli), 98, 60

tannic acid staining and extraction of enzymes in electrophoresis, 95, 575

M

Macroglobulin a,, purification of inhibitor using zinc chelate

chromatography (human), 99, 415 Macromolecules


interferometric technique for determination of electrophoretic mobility (bovine, human), 96, 355

ligand equilibrium binding constants, quantitative uses of affinity chromatography (review), 97, 1

Magnesium calcium concentration in presence of NAD+-linked

isocitrate dehydrogenase, 95, 62 ion depletion, affinity constants for 1:l complexes

with N-(2-acetamidohminodiacetic acid, 95, 527 ion, precipitation of vitellogenin from plasma of

estrogen-treated vertebrates, 97, 145 Magnetic resonance spectrometry (MRS)

determination of nitrilases and amidases activities, 95, 436

Malate dehydrogenase L-malate-2-d prepared by coupled synthesis of

NADD to reduction of oxaloacetate, stereoselective preparation of deuterated reduced nicotinamide adenine nucleotides, 96, 334

Malic enzyme chromatographic purification from heart mito-

chondria (bovine), 100, 299 Malondialdehyde

hydrolysis of Y-labeled tetraethoxypropane, 99,458

Maltopentaose isolation by chromatography on granulated hy-

droxylapatite (corn syrup), 92, 351 Maltose

specific labeling in reducing glucose moiety (Spina- cacia oleracea), 96, 12

Mandelate racemase kinetic assay using circular dichroic measurements,

94, 329 Manganese

digestion of muscle biopsies for trace analysis using organic tissue solubilizer (human), 98, 315

ion depletion, affinity constants for 1:l complexes with N-(2-acetamido)iminodiacetic acid, 95,527

Mannolipids chromatographic separation of mannosylretinyl-

phosphate from dolichylmannosylphosphate (rat), 97, 298

Mannosylretinylphosphate separation from dolichylmannosylphosphate

chromatographically (rat), 97, 298 Mapping

numerical, mean electrophoretic mobilities and cor- responding confidence limits for protein repre- sented in two-dimensional electrophoretic pat- tern, 97, 210

peptide, proteins solubilized from densonucleosis virus (Galleria mellonella), 99, 97

thin-layer, labeled peptides via reductive methylation, 94, 150

tryptic

characterization of proteins and peptides by high- performance liquid chromatography and fluorescence monitoring, 95, 117

peptide, comparison of techniques for protein isolation and radioiodination, 96, 84

Mass spectrometry -analyzed ion kinetic energy, identification of in-

dividual steroids in biological matrices, 96, 104 automated Edman degradation with large sequencer

cup and high-speed drive, 99, 242 chemical ionization

-chromatography, estimation of sphingolipids with reference to ceramides of plasma (human), 97, 323

identification of trimethylsilylated dipeptides, 92, 525

computerized assay for sulindac and its metabolites in plasma (human), 95, 579

determination of cytokinins, 92, 1 t 1 -gas chromatography

analysis of phenylethylamines in brain (rat), 96, 308

application to analysis of prostaglandin E, in biological media (rabbit), 98, 455

characteristics and assay of tele-methylhistamine, 96, 56

detection of 5-methylcytosine and quantitation of S-methylcytosineicytosine ratio in DNA, 94,297

selected ion recording, determination of nicotine in biological fluids, 94, 411

micromethod analysis of amino acids, 92, 426 micromethod for determination of 15N enrichment

in plasma lysine, 94, 242 15N analysis, reduction of plant tissue nitrate to

nitric oxide, 97, 131 quantitative analysis of 16,16-dimethylprostaglandin

EZr 100, 109 secondary ion, standards for analysis of soft bio-

logical tissue, 92, 213 single peak monitoring of endogenous retinoic acid,

demonstration in normal physiological conditions (human), 98, 402

Median method for fitting single exponential to biological

data described, 94, 270 Membranes

adipocyte plasma isolation using diazotized diiodosulfanilic acid, 96, 236

antibody-peroxidase binding to fragments, chromatographic quantitation (human, rabbit), 98, 53

basement: accelerated, single-column procedure for amino acid analysis (human, rabbit), 99, 427

cholesterol/phospholipid molar ratios, 95, 344 component radioiodination, automatic sample-

changer attachment for radioactivity counters, 96, 403

enzymes, electrical activity, 96, 250


protein, radioimmunological screening method (Escherichia calf), 97, 153

purple, electrophoretic mobility on a sucrose gradient (Halobacterium halobium), 92, 228

separation of small vesicles from suspension, 96,384 solvent polymeric electrodes, measurement of ion

activities, 97, 3 15 Mercury

determination in tissues, effect of selenium (mouse, rabbit), 98, 287

Metallothioneins electrochemical approach to quantitation and char-

acterization, 100, 343 hepatic, radioimmunoassay with labeled zinc thio-

nein (rat), 98, 89 Metals, see also specific metals

analysis, application of atomic absorption spectrometry, 94, 386

ion chelation and complex formation, spin-label assay, 99, 175

Methanol heptane:chloroform, calorimetric determination in

subcellular organelles, 97, 403 Methanolysis

of cholesteryl esters, 94, 402 Methionine

carbon-14 labeled, estimation of turnover of spermidine (Anacystis nidulans), 95, 73

estimating amount by reaction with chloramine-T, 93, 419

metabolism, synthesis of radioactive 3-methylthiopropionate and other alkylthio fatty acids, 98, 281

Methionine-cY-naphtyl ester substrate for esterproteases of submandibular gland

(mouse), 93, 251 Methionine sulfoxide

reduction of sulfoxides in peptides and proteins, 98, 36

3-Methoxytyramine specific activity measurement in rat striatum, 92,82

Methoxy-N-acetyldopamine rapid radioenzymatic assay (gastropods), 96, 175

3-Methoxy-4-hydroxyphenylethyleneglycol sulfate fluorometric simultaneous determination with

noradrenaline in discrete brain regions (rat), 97, 352

Methyl anthranilate reductive amination of pyridoxal and its 5’-phos-

phate, fluorometric assay, 96, 426 Methyl glycosaminide

N-acetylation, 94, 36 Methyl green

localization of DNA in metrizamide gradients, 93, 306

Methyl groups purification of ribosomal RNA (plant), 100, 70

Methylation reductive, thin-layer mapping of labeled peptides,

94, 150 3-Methyl-2-benzothiazolone hydrazone

calorimetric determination of erythrocyte surface sialic acid residues, 97, 346

3-Methyl-2-benzothiazolone hydrazone hydrochloride quantitation of glycosaminoglycan hexosamine,

98, 478 O-Methylation

chromatographic product determination, assay of catechol O-methyltransferase, 98, 452

S-Methylcysteine metabolism, synthesis of radioactive 3-methylthio-

propionate and other alkylthio fatty acids, 98, 281

5-Methylcytosine detection in DNA by gas chromatography-mass

spectrometry, 94, 297 Methylene blue

liberation of labile sulfur from ferredoxins by alkaline zinc reagent, 95, 452

Methyl-o-D-ghrcopyranoside removal of concanavalin A from o-1-antitrypsin,

93, 180 7-Methylguanine

chromatographic analysis in DNA (bovine, Esch- erichia coli), 97, 203

7-Methylguanosine -containing 5’-termini of mRNA, determination by

NaB[3H], labeling and chromatography (rabbit), 98, 102

I-Methylhistamine fluorometric analysis, 94, 43 1

tele-Methylhistamine gas chromatography-mass spectrometry char-

acteristics and assay, 96, 56 3-Methylindole

chromatographic determination in rumen liquor, plasma, and tissue (ruminants), 99, 324

N-Methylmercaptoacetamide denaturing agent, reduction of methionine sulfoxide

to methionine in peptides and protejns, 98, 36 Methylmercuric hydroxide

electrophoresis of nuclear RNA and mRNA (HeLa cells, mouse), 99, 146

electrophoretic fractionation of RNAs by partial denaturation, 99, 200

I-Methylnicotinamide 4- and 6-pyridones, preparation by alkylation, 96,

189 distinguishing nicotinamide riboside from, 93, 384

I-Methylnicotinic acid preparation by alkylation, 96, 189

Methyl-N-pyridoxyl anthranilate fluorometric assay of pyridoxal and pyridoxal 5’-

phosphate, 96,426


5Methyltetrahydrofolic acid peracid cleavage at Co-N” bridge, 92, 266

3-Methylthiopropionate radioactive, synthesis based on degradation of

labeled methionine, 98, 281 4-Methylumbelliferyl-a-o-N-acetylneuraminate, see

Sodium Metrizamide

gradients, quantitation for DNA localization in, 93, 306

Microbial populations micromethod analysis of amino acids using gas

chromatography and mass spectrometry, 92,426 Microfluorometry

DNA determination in biological material using 33258 Hoechst, 100, 184

Microsomes calcium uptake, cardiac sarcoplasmic reticulum

isolation from heart (bovine), 99, 41 chromatographic resolution of benzo[a]pyrene

metabolites using organic amines and application to marine hepatic metabolites (Srenoromus vet&o/or), 99, 129

Microtubules brain protein, polymerization kinetics during

purification cycles following isolation without glycerol (bovine), 98, 64

protein, purification from brain and comparison of assembly requirements for neuronal microtubules (bovine, hog), 96, 225

-tubulin equilibrium in cultured cells, biochemical determination, 96, 155

Microwave -induced helium plasma emission spectrometry,

95, 8 Mitochondria

calorimetric method for determination of long-chain free fatty acids, 97, 403

heart, chromatographic purification of malic enzyme (bovine), 100, 299

hepatocyte, intact, isolation from liver cells (rat), 93, 200

liver, calcium metabolism (rat), 93, 430 myocardial subcellular organelles, ATPase activity

(canine), 96, 293 oxidative phosphorylation, selective inhibition of

adenylate kinase activity by P’,P5-di-(adenosine-5’)-pentaphosphate (rat), 96, 7

techniques for biochemical respiration measurements, 95, 416

Mitogens effect of carrier ampholytes on quiescent and

stimulated diploid lung fibroblasts (human), 98, 27

Molecular sieving efficiency influenced by the chain length of single

polymer molecules, 92, 99

Molecular weight calculated for proteins in high ionic strengths, con-

tribution of apparent isopotential specific volume (Homarus americanus), 98, 481

chromatographic estimation for protein polypeptide chains using guanidine hydrochloride, 97, 65

estimation for proteins by electrophoresis in ab- sence of denaturing agents, 98, 160

measurements of electrophoretic mobilities of proteins in presence of sodium dodecyl sulfate, 97, 210

protein subunit, electrophoretic estimation using cetyltrimethylammonium bromide polyacrylamide, 92, 411

separation of proteins and peptides with high- pressure liquid chromatography column, 97, 176

size determination of enzymes by radiation inactivation, 92, 2

spectrin bands, separation by preparative slab electrophoresis technique, 92, 130, erratum, 95,587

Monomers micrococcal nuclease digested for fractionation and

purification of nucleosomes, 92, 420 Monooxygenase

separation of acetanilide and chromatographic determination of acetanilide 4-hydroxylase activity (mouse), 96, 201

Mucopolysaccharides spectrophotometric estimation of concentrations,

99, 183 Mucopolysaccharidoses

fluorimeter for titrimetric assay of glycosaminoglycans, 94, 378

Muscle cardiac, separation of hemoglobin by subunit-

exchange chromatography, 92, 473 determination of phosphenolpyruvate carboxy-

kinase activity with deoxyguanosine 5’-diphosphate as nucleotide substrate (rabbit), 96, 279

digestion of biopsies for trace metal analysis using organic tissue solubilizer (human), 98, 315

fiber, fluorimetry using auxiliary-coupling enzymes to assay ATPase activity (rabbit), 92, 375

skeletal bicarbonate tissue measurement (rat), 95, 183 differential binding of contractile proteins to

cibacron blue F3GA-sepharose affinity columns (rabbit), 95, 317

separation of hemoglobin by subunit-exchange chromatography, 92, 473

smooth, separation of hemoglobin by subunit- exchange chromatography, 92, 473

Mutants chromatographic and bioautographic method for

detectin L-carnitine and its derivatives (Torulop- sis bovina), 96, 322

Mutarotation


lag, optimizing coupled enzyme assay, 99, 142 Myoglobin

attachment of tryptophanyl peptides to 3-aminopropyl-glass suited for subsequent solid-phase degradation (whale), 97, 56

oxygenated, sources of free dissolved O2 at low concentrations for experiments with bacteria (Rhizobium), 96, 165

spectrophotometric determination in cardiac, smooth, and skeletal muscle, 92, 473

Myosin interactions of contractile proteins with free and

immobilized cibacron blue F3GA (rabbit), 95, 317

polyacrylamide gel electrophoresis, relations between chains, 95, 133

N

NAD’ (oxidized nicotinamide adenosine dinucleotide) analysis with sodium [3ZP]pyrophosphate and

NAD-pyrophosphorylase, 97, 309 intracellular, quantitative extraction and estimation

(Escherichia coli), 99, 22 -linked isocitrate dehydrogenase, calcium concentra-

tion in presence of magnesium and chelating effecters, 95, 62

NADH (reduced nicotinamide adenine dinucleotide) enzymatic method for measurement of GTP and GDP

in biological samples, 95, 512 intracellular, quantitative extraction and estimation

(Escherichia cdl), 99, 22 microfluorometric enzymatic assay for determina-

tion of ammonia, 95, 507 peroxidase-catalyzed oxidation, determination of

LDH in blood serum (human), 97, 255 stereoselective preparation of deuterated reduced

nicotinamide adenine nucleotides and substrates by enzymatic synthesis, 96, 334

NADP+ (oxidized nicotinamide adenine dinucleotide phosphate) intracellular, quantitative extraction and estimation

(Escherichia coli), 99, 22 recycling analysis using glucose&-phosphate de-

hydrogenase-glutathione reductase system, 99, 297

NADPH (reduced nicotinamide adenine dinucleotide phosphate) deuterated, stereoselective preparation by enzy-

matic synthesis, 96, 334 intracellular, quantitative extraction and estimation

(Escherichia coli), 99, 22 recycling analysis using glucose&phosphate de-

hydrogenase-glutathione reductase system, 99, 297

Nephelometry laser, quantitation and differentiation of lectin-gly-

coprotein complexes, 96, 391

Neuraminidase application to cultured fibroblasts, 95, 222 fluorimetric assay with sodium substrate (Vibrio

chokrae), 94, 287 Neurons

microtubules, assembly requirements compared to microtubules protein (bovine, pig), 96, 224

submaxillary gland growth factor, isolation of a! and y subunits (mouse), 92,482

Neurophysins chromatographic purification on methionyl-

tyrosyl-phenylalanyl-aminohexyl-agarose, 97, 302

Neurospora enzyme assays using permeabilized cells, 92, 356 N. crassa

isolation and storage of plasma membranes, 95, 554

Nickel ion depletion, afhnity constants for 1:l complexes

with N-(2-acetamido)iminodiacetic acid, 95,527 Nicotinamide

separation of excretory metabolites, 96, 189 Nicotinamide methyltransferase

radioenzymatic assay for dopamine and N-acetyldopamine (gastropods), 96, 175

Nicotinamide ribosides distinguishing from 1-methylnicotinamide, 93, 384

Nicotine determination in biological fluids at picogram levels,

94,411 rapid technique for semiquantitative mass screening

by autoradiographic immunoassay, 92, 147 Nicotinic acid

separation of excretory metabolites, 96, 189 Ninhydrin

methionine degradation, synthesis of radioactive 3- methylthiopropionate and other alkylthio fatty acids, 98, 281

reaction amino acids, quantitation of proteins, 96, 130 chromatography of diamino acids on single-

column amino acid analyzer, 98, 293 Nitrate reductases

cross-reaction with antibodies raised to component I of nitrogenase (Azotobacter vine/and?), 95, 24

immunoelectrophoretic determination (Neurospora crassa), 95, 97

Nitrates metal, vaporization characteristics, 95, 8 reduction to nitric oxide for mass spectrometric iSN

analysis in plant tissue, 97, 131 Nitric oxide

reduction of plant tissue nitrate for mass spectrometric 15N analysis, 97, 131

Nitrilases spectrometric determination of activity, 95, 436

4-Nitroanilide


assay for serine proteases, merits of chromophoric and fluorogenic leaving groups (human, porcine), 99, 53

Nitrocellulose filter binding

protein-nucleic acid, 93, 204 quantitation of histidyl-tRNA-ATP phos-

phoribosyltransferase complex, 92, 22 Nitrogen

-based protein assay procedures, variations in sensitivity of proteins, 99, 136

cavitation, cell disruption followed by isolation of intracisternal type A-particles and associated RNA (mouse), 97, 8.5

fixation, systems utilizing oxygenated leghemoglobin and myoglobin as sources of free dissolved 0, at low concentrations (Rhizobiurn), 96, 165

Nitrogenase component I, antibodies cross-reacted with protein

and nitrate reductases (Azorobacter vinelandii),

95, 24 p-Nitrophenylacetate

hydrolysis, rapid-mixing apparatus for continuous- flow studies, 97, 428

p-Nitrosodimethylaniline photometric assay for alcohol dehydrogenase ac-

tivity in blood serum (equine, human), 99, 65 N’-Nitrosonomicotine

synthesis of derivative, radioimmunoassay (rabbit), 95, 387

Noradrenaline fluorometric simultaneous determination with 3-

methoxy-4-hydroxyphenylethyleneglycol sulfate in discrete brain regions (rat), 97, 352

Norepinephrine radioenzymatic assay for dopamine and N-acetyl-

dopamine (gastropods), 96, 175 Norgestrel

analysis of birth control pills, mass-analyzed ion kinetic energy spectrometry, 96, 104

Nornicotine nitrosation rate constants, radioimmunoassay of N’-

nitrosonornicotine, 95, 387 Nuclease

resistance, DEAE disk assay for photoreactivation of pyrimidine dimers in [$H]DNA (Escherichia

coli), 97, 376

Nuclease S 1 isolation of foldback DNA, 96, 144

Nucleophiles micromethod for assay of primary amines and pro-

teins with fluorescamine, reexamination of conditions of reaction, 99, 379

Nucleosides radioactive triphosphates, synthesis, 95, 429 reversed-phase liquid chromatography, practical

aspects applied to biochemical and biomedical research (review), 99, 1

triphosphate pools determined, chromatographic isolation of 5-phosphoryibosyl-a-l-pyrophosphate (Salmonella typhimurium), 98, 254

Nucleosome monomers, fractionation and purification by sodium

phosphate, 92, 420 Nucleotidase

assay for hypoxanthine-quanine phosphoribosyltransferase enzyme by DEAE-filter disk (human fibroblast), 92, 403

5’-Nucleotidase assay, adenosine electrode, 95, 479 radiometric assay, 95, 497

Cyclic 3’,5’-nucleotide phosphodiesterase spectrophotometric continuous assay, 97, 239

Nucleotides adenine, microprocedure extraction for high-

performance liquid chromatography analysis, 93, 227

cyclic, purification of regulator of phosphodiesterase from liver utilizing calcium-binding ability (rat), 96, 407

DNA sequence, approaches to analysis (review), 98, 243


specificity of pyruvate kinase and phosphenolpyruvate carboxykinase (rabbit, guinea pig), 96, 279

0

Oligoadenylates analysis on RPC-5 using a duel-column technique,

92, 276 Oligodeoxyribonucleotides

electrophoretic determination of relative binding constants of biologically active, intercalating fluorescent stains, 95, 329

Oligonucleotides inhibition of nuclease contaminant in alkaline

phosphatase (Escherichia co/i), 95, 458

prediction of electrophoretic mobilities on neutral paper, 98, 8

two-dimensional fractionation, gradient thin-layer chromatography as alternative to homochromatography, 98, 346

Oligosaccharides acetylated, reverse-phase chromatographic separa-

tion, 97, 176 action of diplococcal endo-@-galactosidase (review),

loo, 1 glycoprotein-derived, separation of by thin-layer

chromatography, 93, 167 isolation of maltopentaose by chromatography on

granulated hydroxylapatite (corn syrup), 92,35 1 Optics

continuous scanning in polyacrylamide gel electro-

412 CUMULATIVE

phoresis, estimation of apparent diffusion coefficient of P-lactoglobulin B, 97, 120

convection, mixing device for quasielastic light scattering studies of reacting systems, 99, 217

coupled assay for determination of adenine in mixtures, 98, 213

rotary dispersion, purpose and technique of the Keston polaramic attachment to the Beckman DU spectrophotometer, 92, 55

synchronously pumped krypton ion dye laser fluorescence system, biochemical application, 97, 17

Organic acids tricarboxylic acid cycle, purification and quantifica-

tion, 95,311 Ouabain

receptor-binding assays, application of miniature ultracentrifuge, 96, 395

Oxalate from progenitors, requirements of specificity, re-

covery, and generation, 94,470 pool size, determination by stable isotope dilution,

94,477 Oxalic acid

action on heme proteins, 92, 11 urinary, determination by stable isotope dilution,

94,477 Oxidase

micro determination of polyamines (snake venom), 92, 453

Oxidation cleavage of folates, critical appraisal, 93, 91 peroxidase-catalyzed, NADH as indicator reaction

in determination of LDH in blood serum (human), 97, 255

Oxipurinol chromatographic simultaneous analysis with

hypoxanthine, xanthine, allopurinol, and uric acid, 98, 18

2-Oxoghttarate aminotransferase :L-aspartate, enzymatic and coupling assay, 100, 20

Oxygen consumption, radiorespirometer for continuous

monitoring of embryo development (chicken), 98, 375

continuous and discontinuous photometric analysis in gases and fluids, 92, 255

electrode membrane-covered, biochemical respiration

measurements, 95, 416 measurement of ion activities, 97, 315 polarographic, characteristics of electronic differ-

entiator facilitating continuous monitoring changes in oxygen concentration, 96, 341

system for a spectrophotometer cuvette, 93, 386 uptake, simultaneous measurement with aggrega-

tion, secretion, proton production, and intracellular metabolites in platelet suspension, 97, 387

SUBJECT INDEX

Oxygenase activity; instrumental improvements for rapid, de-

tailed kinetic studies, 96, 334

P

Palmitoyl-coenzyme A chromatographic determination of CoASH and ap-

plication in assay of long-chain acyl-CoA derivatives, 96, 181

Pancreas elastase, study of merits of various chromophoric

and fluorogenic leaving groups in assay for serine proteases (porcine), 99, 53

oral pancreatic function test, assay for measurement of urinary p-aminobenzoic acid (human), 98, 13

Paper chromatography analysis of cY-tocopherol in liver employing direct

attack on tissue with alcoholic alkali in presence of hydroquinone and pyrogallol (ovine), 97,264

mutant utilized for detecting L-carnitine and its derivatives (Torulopsis bovina), %, 322

specific characterization of trace amounts of uridine nucleotides (hamster), 95, 245

Parathyroid hormone bovine, electrolytic iodination, 92, 61

Partition assay for epoxide hydrase activity, 92, 16

Pectin galactose oxidase tritiated-potassium borohydride

method for labeling pectic polysaccharides (grapefruit), 98, 429

Penicillin estimation of p-lactam antibiotics, 95, 413

Pentamethylmelamine chromatographic-spectrometric identification in

plasma as hexamethylmelamine metabolite (mouse), 99, 441

Pepsinogen semiautomated determination in plasma (bovine),

99, 321

Peptidase amino-procollagen type 1, determination of kinetic

parameters and endopeptidase activity (bovine), 95, 406

collagenase-like activity, fluorescence assay, 100,43 Peptides

adenine nucleotide binding sites of labeled enzymes, three-step isolation procedure, 95, 39

analyzer and monitor for rapid microscale separation, 98, 178

bond-containing molecules, calibration of hydrogen- deuterium exchange by ultraviolet spectrophotometry, 92, 517

characterization by high-performance liquid chromatography and fluorescence monitoring of tryptic digests, 95, I17


chromatographic molecular weight separation, 97, 176

collagen cyanogen bromide, analysis using electrophoresis, 92, 183

COOH-terminal degradation, chromatographic isocratic resolution of thiohydantoins, 95, 494

y-cyanogen bromide, chromatographic separation of fetal hemoglobin (human), 96, 113

estimation of molecular weight by exclusion chromatography, 94, 228

fingerprinting, extraction from Coomassie blue- stained sodium dodecyl sulfate-polyacrylamide gels, 96, 352

iodination, comparison of methods, 92, 46 labeled, thin-layer mapping via reductive methyla-

tion, 94, 150 nucleosidyl, chromatographic purification on dihy-

droxyboryl-substituted polyacrylamide and cellulose (rabbit), 99, 85

purification by gel filtration in aqueous pyridine- ammonia, 95, 397

reduction of methionine sulfoxide to methionine, 98, 36

separation from n-amino acids on copper-Sephadex (cheese, yeast), 97, 367

sequential analysis by phenylisothiocyanate procedure for degradation, 96, 469

silver stain for detection in polyacrylamide gels, 98, 231

synthetic, o-phthalaldehyde and the fluorogenic detection, 94, 371

trinitrobenzenesulfonic acid method for detection in chromatographic column effluents, 96, 317

tryptophanyl, attachment to 3-aminopropyl-glass suited for subsequent solid-phase degradation, 97, 56

Peracetic acid cleavage of 5methyltetrahydrofolic acid at Q-N’*

bridge, 92, 366 Performic acid

bicarbonate tissue measurements (rat), 95, 183 Periodate

isotachophoresis for oxidation analysis of carbohydrate, 98, 417

oxidation of ribonucleotides, 99, 268 -thiobarbaturic acid assay for hyaluronate lyase,

determination of hyaluronate, 96, 474 Permeabilization

enzyme assays using cells of Neurospora, 92, 356

Peroxidase -L-amino acid oxidase system, protease activity

measurement-(Aspergilfus japonica), 97, 11 -antibody binding to membrane fragments, chromat-

ographic quantitation (rabbit, human), 98, 53 -catalyzed oxidation for NADH as indicator reaction

for repetitive determinations, determination of LDH in blood serum (human), 97, 255

coupled assay, measurement of diamine oxidase activity in amniotic fluid (human), 96, 411

cytochemical staining using 3,3’,5,5’-tetramethylbenzidine dihydrochloride, 98, 388

label in chemiluminescence enzyme immunoassay of cortisol, 97, 248

Peroxides lipid, assay in tissues by thiobarbituric acid reac-

tion (rat), 95, 351 PH

chromatographic fractionation of dG homooligonucleotides, 99, 213

influence on arsenoza III, 96, 70 mitogenic and inhibitory effects of carrier ampho-

lytes on quiescent and stimulated diploid lung fibroblasts (human), 98, 27

prediction of electrophoretic mobilities of nucleotides on neutral paper, 98, 8

reduction of methionine sulfoxide to methionine in peptides and proteins, 98, 36

separation of alpha and beta anomers of hexosamines, 96, 77

Phenanthrene enzymatic hydration of K-region arene oxides,

97, 340 1, IO-Phenanthroline

catalytic method for determination of trace amounts of iron in biological material, 99, 28

Phenazine ethosulfate preferred electron acceptor to phenazine methosul-

fate in dye-linked enzyme assays, 99, 112 Phenazine methosulfate

dye-linked enzyme assay, phenazine ethosulfate as preferred electron acceptor, 99, 112

p-Phenetidine hydrochloride oxidized, catalytic method for determination of

trace amounts of iron in biological material, 99, 28

Phenol extraction, purification of RNA resolved by ana-

lytical and preparative electrophoresis, 98, 358 Phenol sulfotransferase

enzyme transferred 3’-phosphoadenosine-5’-phosphosulfate, fiber binding assay, 95, 82

Phenolphthalein spectrophotometric assay in biological fluids,

95. 465 Phenylacetic acid

excretion and mass fragmentography (human), 99, 283

Phenylethanolamine brain concentrations quantitated using selected ion

monitoring in chemical ionization mode (rat), 96, 308

Phenylethanolamine-N-methyltransferase one-step method for radiometric analysis in adrenal

chromaffin cells (bovine), 99, 281 Phenylethylamines


gas chromatography-chemical ionization mass spectrometry analysis in brain (rat), %, 308

Phenylisothiocyanate procedure for degradation of peptides, sequential

analysis, 96,469 o-Phenylphenol

fluorometric determination of trioses, 94, 1 Phenylthiohydantoin

amino acid automated Edman degradations with large se-

quencer cup and high-speed drive, 99, 242 chromatographic analysis on DuPont Zorbax

cyanopropylsilane columns, 100, 335 Phosphatases

alkaline conversion of phosphorylcholine to inorganic

phosphate for assay of phospholipase C activity (Clostridium petiingens), 97, 43

inhibition of nuclease contaminant (Escherichia co/i), 95, 458

radiometric assay for 5’-nucleotidase, 95, 497 placental alkaline, measurement of bound peroxi-

dase-antibody in membrane fragments (human, rabbit), 98, 53

Phosphate buffer, effect in preparation of collagen substrates

for cell attachment (Chinese hamster), 94, 308 inorganic

assay for nanomole amounts, 100, 95 conversion of phosphorylcholine using alkaline

phosphatase, assay for phospholipase C activity (Clostridium perfringens), 97, 43

modification in enzymatic assay, 95, 520 synthesis and purification of [ l-32P]fructose- 1,6-

bisphosphate with high specific radioactivity, 96, 500

synthesis of &*P-nucleoside triphosphates, 95, 429

released without deproteinization, automated assay of phosphohydrolases by measuring, 94, 397

Phosphatidyl choline solubilization, electrophoretic analysis of basic pro-

teins, 108, 289 Phosphodiesterases

assay, AMP electrode, 95, 479 cyclic nucleotides, purification of regulator utilizing

calcium-binding ability (rat), %, 407 cGMP and CAMP, measurement, 100, 244 spectrophotometric assay for cyclic 3’,5’-nucleotide

phosphodiesterase, 97, 239 Phosphoenolpyruvate

detection of enolase activity on polyacrylamide gels, 98, 226

Phosphoenolpyruvate carboxykinase activity, determination with deoxyguanosine Y-

diphosphate as nucleotide substrate, 96, 279 6-Phosphogluconate dehydrogenase

DNA, protein and enzyme activity, fluorometric assay (rat), 94, 166

Phosphoglycerate kinase preparation from Saccharamyces cerevisiae using

cibacron blue-Sepharose 4B pseudoaffinity chromatography, 93, 46

Phosphohydrolases automated assay by measuring released phosphate

without deproteinization, 94, 397 Phospholipase C

activity, assay using alkaline phosphatase to convert phosphorylcholine to inorganic phosphate (Clostridium perfringens), 97, 43

Phospholipids reduced cholesterol/phospholipid molar ratios in

cell membranes (human), 95, 344 separation of small vesicles from suspension, 96,

384 vesicles, spin probe for detecting phase transi-

tions, 94, 322 Phosphorescence

simultaneous measurement with fluorescence using synchronously gated photon counters, 97, 158

5-Phospho-o-ribose-a-I-pyrophosphates chromatographic determination of pools (Salmonella

typhimurium), 98, 264 5-Phosphoribosyl-a-1-pyrophosphate

determination of cellular pools, chromatographic isolation (Salmonella typhimurium), 98, 254

5-Phosphoribosyl-a-1-pyrophosphate synthetase assay of activity, chromatographic isolation of

5-phosphoribosyl-o-I-pyrophosphate (Salmo- nella typhimurium), 98, 254

Phosphorylcholine conversion to inorganic phosphate using alkaline

phosphatase, assay for phospholipase C activity (Clostridium per-ingens), 97, 43

Phosphotransacetylase purification by afhnity chromatography (Clostridium

kluyveri), 95, 2 spectrophotometric cycling assay for reduced co-

enzyme A and its esters in tissue (mouse), 97, 103

Phosphotransferase system, modified assay procedures (enteric bac-

teria), 95, 293 Photochemistry

determination of carbon monoxide levels in blood, 94, 431

reactions of bis-azido compounds, application to preparation of enzyme-polymer film, 97, 320

Photolysis apparatus, determination of carbon monoxide levels

in blood, 94, 431 Photometry

assay for alcohol dehydrogenase activity in blood serum (equine, human), 99, 65

continuous and discontinuous analysis of oxygen, carbon dioxide and carbon monoxide in gases and fluids, 92, 255

Photoreactivation


pyrimidine dimers in [3H]DNA, DEAE disk assay (Escherichia coli), 97, 376

o-Phthalafdehyde detection agent in fluorometric microbore amino

acid analyzer, 96, 298 detection, fluorometric amino acid analysis, 93, 174 determination of oxidized glutathione, fluorimetric

assay of blood platelets (human), 98, 154 fluorometric assay of proiine reductase, 95, 44 fluorometric quantitation of amino sugars in pico-

mole range, 98, 190 reaction with peptides to yield fluorescent deriva-

tives dependent upon free e-amino group of lysine, 94, 371

Phycobiliproteins determination of thioether-linked cysteine residues,

92, 489 Phycocyanin

determination of thioether-linked cysteine residues, 92, 489

Phycoerythrin determination of thioether-linked cysteine resi-

dues, 92, 489 Picrate

test, determination of ammo groups on amino propyl glass, 94, 60

Placenta peroxidase-antibody binding to membrane frag-

ments, chromatographic quantitation (human, rabbit), 98, 53

Plant spectrophotometric estimation of arginine decar-

boxylase (oat seedlings), 92, 33 1 Plasma

adipocyte membranes, isolation using diazotized diiodosulfanilic acid, 96, 236

op macroglobulin and lyl proteinase inhibitor, purification using zinc chelate chromatography (human), 99, 415

automated assay of vitamin C (rat), 92, 510 ceramides, chromatographic-spectrometric estima-

tion of sphingolipids (human), 97, 323 chromatographic and precipitative isolation of vitel-

logenin (Xenopus Iaevis, vertebrates), 97, 145 chromatographic determination of hexamethyl-

melamine (mouse), 99, 441 chromatographic electron-capture detection of

guanethidine, 96, 118 calorimetric determination of sulfhydryl content,

93, 98 computerized spectrometric assay for sulindac and

its metabolites (human), 95, 579 estimation of dehydroascorbic acid in diabetic

by isolation as 2,4-dinitrophenylhydrazone derivative (human), 98, 368

fluorometric assay for urea, 98, 132 measurement of haptoglobin concentration by spec-

trometry (human), 99, 42 1 measurement of histamine (rat), 94, 425

membranes actin, isoelectric focusing (chick fibroblasts), 95,

236 isolation and storage (Neurospora crussa), 95,

554 radiometric assay for S’nucleotidase (rat), 95,497

oxalogenesis occurs during assay, 94, 470 quantitative analysis of 16,16-dimethyl-prostaglan-

din E2 using deuterated carrier and gas chromatography-mass spectrometry, 100, 109

radioimmunoassay of vasoactive intestinal polypeptide (porcine), 97, 286

release radioimmunoassay for determination of chorionic somatomammotropin levels (human), 99, 121

semiautomated determination of pepsinogen (bovine), 99, 321

Plasmids detecting foreign DNA using lysozyme and Triton

X- 100 in conjunction with hybridization (Esch- erichia coli), 98, 60

Plasminogen activation, assay with 3H-peptidyl anilides, 97,

269 Plastocyanin

chemically modified, preparation and characterization, 95, 254

Platelets application of fluorimetric microassay for deter-

mination of glutathione peroxidase activity (human), 98, 154

suspension, simultaneous measurement of aggregation, secretion, oxygen uptake, proton production, and intracellular metabolites, 97, 387

Polarization fluorescence, assay of proteolytic enzymes, 92,222

Polarography continuous coupled assay for trehalase, 94, 360 oxygen electrode, characteristics of electronic dif-

ferentiator facilitating continuous monitoring changes in oxygen concentration, 96, 341

Polyacrylamide beads, chromatographic fractionation of histones

(cricket), 94, 220 liquid, properties of in the presence of cellulose

acetate, 92, 99 Polyacrylamide gel electrophoresis

analysis of collagen cyanogen bromide peptides, 92, 183

analysis of glycopeptides as borate complexes, 97, 438

apparatus, sodium dodecyl sulfate, recovery of proteins, 97, 229

automatic sample-changer attachment for radioactivity counters, 96, 403

continuous flow, RNA preparative (Escherichia coli), 93, 299

continuous optical scanning, estimation of apparent

416 CUMULATIVE

diffusion coefficient of P-lactoglobulin B, 97, 120

estimation of protein subunit molecular weights using cetyltrimethylammonium bromide polyacrylamide, 92, 411

high-voltage, application to sequencing of nucleic acid, 100, 129

improved design combining all parts into one integral unit, 100, 87

linear gradient gels, estimation of protein molecular weights, 98, 160

miniaturized system, 99, 233 problems and solutions for detection of arylsul-

fatase A activity, 97, 36 proteins, studies and critique of amido black lOB,

Coomassie blue R and G, and fast green FCF as stains, 96, 262

relations between chains of myosin, 95, 133 sodium dodecyl sulfate

effect of composition of preparations on renaturation of enzymes, 100, 357

purification and characterization of tumor virus glycoprotein (mouse), 94, 40

purification of restriction endonuclease Pal1 (Providencia alcalifaciens), 99, 207

recovery of functional proteins, 99, 170 removal of Triton X-100 from protein solution,

97, 116 simultaneous isolation of major viral proteins,

98, 329 visualization of protein bands, 93, 257

staining of glycoproteins with fluorescent lectins, 96, 208

tannic acid staining and extraction of enzymes, 95, 575

two-dimensional analysis of proteins, 98, 410 double-label autoradiography for comparison of

complex protein mixtures, 96, 39 separation of cellular proteins, 93, 329

Polyacrylamide gels amino acid spacing in isotachophoresis, 94, 202 casting of continuous-pore gradient slabs without

special equipment, 92, 91 containing thioglycoside ligands, preparation, 95,

260 detection of enolase activity, 98, 226 detection of glycoproteins with thymol and sul-

furic acid, 99, 474 dihydroxyboryl-substituted, chromatographic puri-

fication of nucleosidyl peptides (rabbit), 99, 85 direct visualization of collagens and procollagens,

97, 359 effect on the electrophoresis of sodium dodecyl

sulfate, 92, 243 electrophoretic processing of multiple slabs, 99, 105 fluorographic detection of radioactivity with water-

soluble fluor, sodium salicylate, 98, 132

SUBJECT INDEX

‘H- and W-containing gel slices, one-step liquid scintillation counting method, 99, 161

immunoabsorption, isoelectric focusing and enzymatic characterization combined, barley and malt amylases (rabbit), 93, 261

isoelectric-focusing slab gels, two-dimensional electrophoresis of small-molecular-weight proteins, 99, 33

low-cost scanning densitometer, 100, 15 silver stain for detection of proteins and pep-

tides, 98, 231 -sodium dodecyl sulfate

properties cross-linked with N,N’-diallytartar- diamide, macromolecules isolated, 93, 275

recovery of functional proteins, 99, 170 recovery of proteins, 94, 274, 97, 229

solubilizable, quantitative determination of incorporated protein, 100, 264

Polyamines analysis by high-performance liquid chromatog-

raphy (guinea pig), 93, 423 micro determination with oxidase (snake venom),

92, 453 fluorometric analysis, 94, 431

Polyethyleneimine cellulose application of 5-phospho-u-ribose-a-l-pyrophos-

phate into plates, chromatographic determination of pools (Salmonella typhimurium), 98, 264

Polylysine aggregating suspensions of small vesicles of natural

biological membrane of phospholipid or phospholipid-protein mixtures, 96, 384

Polymerization kinetics during purification cycles following isola-

tion of brain microtubule protein without glycerol (bovine), 98, 64

Polynucleotide phosporylase ribonucleotide diphosphate control, 95, 171

Polyols borate ion interaction, comparative analysis, 95,383

Polypeptides elongation factor II, 8-(6-aminohexyl)-amino-GTP

and -GDP applied as ligands in affinity chromatography (porcine, rat), 99, 189

vasoactive intestinal, radioimmunoassay (porcine), 97, 286

Polysaccharides pectic, labeling by galactose oxidase tritiated-

potassium borohydride method (grapefruit), 98, 429

Polysomes extraction without use of detergent (Escherichia

coli), 99, 454

undegraded, preparation from immature erythrocytes (chicken), 98, 464

Polyvinyl chloride


tubing, measurement of ion activities with solvent polymeric membrane electrodes, 97, 315

Porphobilinogen direct assay for enzymatic formation (human), 97,

277 Potassium

-ion-selective electrode, construction and performance characteristics described, 96, 90

measurement of by stimulation of pyruvate kinase (plant material), 92, 370

selective electrode, measurement of ion activities, 97,315

Potassium borohydride tritiated, galactose oxidase method for labeling

pectic polysaccharides (grapefruit), 98, 429 Potassium tetraborate

concentration on Morgan-Elson color reaction, hexosamine determination, 98, 433

Potentiometry enzyme electrode-based kinetic assay of enzyme

activities, 95, 479 titration, determination of heparin content in

heparin-Sepharose, 95,449 Probe

spin, galvinoxyl, detecting phospholipid phase tran- sitions, 94, 322

Procoagulants venom, differentiator for conversion of spectro-

photometer into coagulometer (Crofulus horridus horridus, Agkistrodon contortrix mokasen), 97, 418

Procollagens direct visualization in polyacrylamide gels, 97, 359 peptidase type 1, determination of kinetic parameters

and endopeptidase activity (bovine), 95, 406 type I, isolation and purification by adsorption to

glass beads (human), 96, 433 type II, purification by covalent chromatography,

94, 2.53 Prolactin

oxidized, reduction of sulfoxides in peptides and proteins, 98, 36

Proline automated fluorometric analysis, 94, 3 13

Proline reductase fluorometric assay with o-phthalaldehyde, 95, 44

Pronase -derived glycoproteins, analysis as borate complexes

by electrophoresis, 97, 438 Prostaglandin cyclooxygenase

instrumental improvements for rapid, detailed kinetic studies, 96, 341

Prostaglandin E, analysis in biological media, application of high-

performance liquid chromatography and gas chromatography-mass spectrometry (human, rat, rabbit), 98, 455

Prostaglandins

biosynthesis, determination using high-performance liquid chromatography and radioimmunoassay, 93, 339

Protamines aggregating suspensions of small vesicles of natural

biological membrane of phospholipid or phospholipid-protein mixtures, 96, 384

a-amino groups, reactions of fluorescamine with chromosomal proteins (herring, salmon), 99,274

Protease K isolation of RNA, 98, 116

Proteases activity measured by means of L-amino acid oxi-

dase-peroxidase system (Aspergillus japonica ), 97, 11

inhibitor, isolation by affinity chromatography (human urine), 92, 383

isolation and purification of type I procollagen by adsorption to glass beads (human), 96, 433

peptidyl derivative of [3H]aniIine as sensitive, stable substrate, 97, 269

trypsin-like, immobilized anhydrotrypsin as biospecific affinity adsorbent for peptides (bovine), 98, 198

use of radioiodinated protein substrates for assay of trypsin and hatching enzyme (Xenopus laevis), 100, 352

Protein A 1251-labeled

detection of immunological binding in leucovorin immunoassay, 95, 472

used as tracer for solid-phase immunoassay of immunoglobin E (human), 93, 207

radioimmunoassay of antibody to 4X 174 DNA.RNA hybrid (Staphylococcus aureus), 98, 74

solid-phase assay for antiactin antibody and actin using (mouse, rabbit), 94, 140

Protein kinases cyclic AMP-dependent, estimation of receptor

capacity, 98, 310 quantitative assay on isoelectric focusing gels, 93,52

Proteinase a,, purification of inhibitor using zinc chelate chro-

matography (human), 99, 415 Proteins, see also speciJic proteins

active sites and purification, quantitative uses of affinity chromatography (review). 97, 1

amino acid spacing in isotachophoresis on polyacrylamide gels, 94, 202

automated assay of phosphohydrolases by measuring release phosphate without deproteinization, 94, 397

bands, visualization in preparative sodium dodecyl sulfate-polyacrylamide gels, 93, 257

basic, electrophoretic analysis using phosphatidyl choline/urea solubilization, 100, 289

binding parameters for interaction with substrates, 92, 123


binding to filter paper disks and determination in calorimetric and radioisotopic assay, effect and significance of sodium hydroxide, 108, 98

-bound 4’-(9-acridinylamino)methanesulfon-m- anisidide, fluorescence assay, 96, 504

brain microtubule, polymerization kinetics during purification cycles following isolation without glycerol (bovine), 98, 64

calculated molecular weight, contribution of apparent isopotential specific volume (Homarus americanus), 98, 481

cell culture, automated fluorometric analysis (rat), 94, 166

cellular, two-dimensional separation by polyacrylamide gel electrophoresis and isoelectric focusing, 93, 329

centrifuge column for desalting solutions, 108, 184 characterization by high-performance liquid chroma-

tography and fluorescence monitoring of tryptic digests, 95, 117

chromatographic molecular weight separation, 97, 176

collagen-like; accelerated, single-column procedure for amino acid analysis (human, rabbit), 99,427

complex mixtures, double-label autoradiography after electrophoresis, 96, 39

concentration using carboxymethyldextran, 98, 353 contractile, interactions with free and immobilized

cibacron blue F3GA. 95, 317 COOH-terminal degradation, chromatographic iso-

cratic resolution of thiohydantoins, 95, 494 denaturation, enzyme stabilization by immobiliza-

tion (review), 93, 1 determination, interference by detergents, 96, 21 dye binding

effects of amido black lOB, Coomassie blue R and G, and fast green FCF, %, 262

estimation of concentration in crude cell and tissue extracts (rat), 94, 160

electric current-generating, lipid-impregnated filters as study tool, 96, 250

electrophoresis behavior during heat-induced carbamylation,

93, 312 estimation of molecular weight using cetyltri-

methylammonium bromide polyacrylamide, 92, 411

polyacrylamides cross-linked with N,N’-diallyltartardiamide, 93, 275

estimation in colored or turbid solutions, 109, 92 estimation of molecular weight by electrophoresis in

linear polyacrylamide gradient gels, 98, 160 estradiol-binding, use of immobilized estradiol anti-

serum, 94, 278 extraction from Coomassie blue-stained sodium

dodecyl sulfate-polyacrylamide gels, 96, 352 fingerprinting, electrophoresis after partial frag-

mentation with CNBr, 97, 382

Folin phenol protein quantitation method of Lowry, Rosebrough, Farr, and Randal (review), 100,201

fractionation, Folate-Sepharose column as affinity absorbent, 98, 184

functional, recovery from sodium dodecyl sulfate- polyacrylamide gels, 99, 170

HeLa, recovery from sodium dodecyl sulfate- polyacrylamide gels, 94, 274

hydrolysates alkaline, determination of free y-carboxyglutamic

acid, 98, 219 analyzer and monitor for rapid microscale separa-

tions, 98, 178 hydrophobic, preparative high-performance liquid

partition chromatography (review), 98, 1 identification in two-dimensional gel electrophoresis

systems, 92, 174 in vitro synthesis by fibroblasts, radioassay, 96, 139 insoluble, quantitative determination by application

of biuret reaction, 95, 340 isolation and radioiodination by tryptic peptide map-

ping, 96, 84 ligand interactions, nitrocellulose filter binding in

quantitation, 92, 22 low potential, electrochemical method for measuring

redox potentials by microcoulometry at controlled potentials, 99, 399

membranes electrofocusing of zwitterionic and nonionic de-

tergents, 95, 201 gas chromatography determination of protein-

bound dodecyl sulfate, 92, 444 metabolism in cultured cells, application of amino

acid analysis, 96, 45 micromethod for assay with fluorescamine, re-

examination of conditions of reaction, 99, 379 microtubules, purification from brain and compari-

son of assembly requirements for neuronal microtubules (bovine, hog), 96, 225

mixtures, radioiodination for electrophoretic analysis, 95, 568

myocardial, effect of sodium desoxycholate on extraction (rat), 98, 278

nuclear, preparation from carcinogen-treated embryo fibroblasts (hamster), 97, 410

-phospholipid vesicles, separation from suspension, 96, 384

polypeptide chains, chromatographic estimation of molecular weights using guanidine hydrochloride, 97, 65

precipitation, disk assay for determination of glycoprotein glycosyltransferases (human), 98, 95

preferential radioiodination of small amounts in presence of excess lipids, 99, 92

prestaining with Coomassie blue G prior to gel electrophoresis, 93, 373

quantitation taking into account amino acid composition, %, 130


quantitative determination in solubilizable polyacrylamide gels, 100, 264

radioimmunological screening method (Escherichia co/i), 97, 153

radioiodinated substrates, use for assay of trypsin and hatching enzyme (Xenopus laevis), 100,352

recovery from sodium dodecyl sulfate-polyacrylamide gels, 97, 229

redox-homogeneous small molecule reaction rates, chronoamperometric determination, 96, 326

reduction of methionine sulfoxide to methionine, 98, 36

removal of sodium dodecyl sulfate by ion-pair extraction, 93, 153

riboflavin-binding, purification of by salt gradient and gel filtration (chicken yolk), 92, 345

scintillation method for tryptophan determination, 3-diazonium-1,2,4-triazole reaction, 99, 464

separation by zonal electrophoresis on density gradients, 93, 41

separation of DNA segments using glass filters, 93, 158

silver stain for detection in polyacrylamide gels, 98, 231

small-molecular-weight, two-dimensional electrophoresis, 99, 33

sodium dodecyl sulfate-solubilized, tissue-specific code based on abundance, 92, 193

solubilized from densonucleosis virus, purification and peptide mapping (Galleria mellonella), 99, 97

solubilized in sodium dodecyl sulfate-mercaptoethanol-Tris electrophoresis buffer, 180, 64

soluble, chromatographic separation of egg yolk (chicken), 94, 53

solution, removal of Triton X-100, 97, 116 spectrophotomettic assay with turbidity correction

of sized immunoglobulin G aggregates, 99, 346 structural analysis, automated Edman degradation

with large sequencer cup and high-speed drive, 99, 242

structure, gel electrophoresis of fluorescent labeled cyanogen bromide cleavage products, 95, 122

subunit-exchange chromatography, behavior of (Y- chymotrypsin, 95, 89

synthesis adsorbent for immune complexes used for assay in

cells and cell-free translations (Staphylococcus aureus), 97, 24

detection of eukaryotic ribosome dissociation factors (wheat germ), 93, 238

thricodermin inhibition, preparation of polysomes and mRNA from erythrocytes (chicken), 98,464

tritium labeling electrophoretically on sodium dodecyl sulfate-polyacrylamide slab gels, 95, 359

turnover, micromethod for determination of 15N enrichment in plasma lysine, 94, 242

two-dimensional analysis, separation according to molecular weight, 98, 410

urinary, concentration and two-dimensional electrophoretic analysis (human), 95, 48

variations in sensitivity to protein assay procedures, 99, 136

viral, simultaneous electrophoretic isolation from RNA tumor viruses, 98, 329

visualization after isoelectric focusing during two- dimensional gel electrophoresis (Smittia spec.), 98, 81

Proteolysis isolation and characterization of proteins solubilized

from densonucleosis virus (Galleria mellonella), 99, 97

Protocatechuate 3,4-dioxygenase acquisition and analysis, computer-controlled laser

spectrophotometer with interactive-graphics data analysis, 96, 456

Psoralens photochemical crosslinking, hairpins stabilized on

denatured simian virus 40 for electron micro- scope visualization, 95, 108

Pteroylpentaglutamate electrochemical reduction yielding fully reduced

tetrahydro derivative, 97, 48 Pteroylpolyglutamates

peracid cleavage of 5-methyltetrahydrofolic acid at C-N”’ bridge, 92, 366

separation and identification by polyacrylamide gel chromatography, 92, 501

Purities base, radiometric assay for 5’-nucleotidase, 95, 497 derivative, radioimmunoassay for antiviral agent

BW248U [9-(2-hydroxyethoxymethyl)-guanine), 98, 319

methylated, chromatographic analysis in DNA treated with chemical carcinogens (rat), 180, 58

dthiopurine, interaction with cellulose mercurial, 92, 324

Pyocyanin new method of preparation and unusual bacterial

sensitivity (Pseudomonas aeruginosa, Proteus morganii), 95, 19

Pyrene enzymatic hydration of K-region arene oxides,

97, 340 Pyridine

-ammonia, use in gel filtration of peptides, 95, 397 nucleotide

fluorescence, reliable rapid-mixing apparatus for continuous-flow studies, 97, 428

-linked fluorometric assays, measurements of glycolytic and tricarboxylic acid cycle metabolites, 97, 394

Pyridones I-methylnicotinamide and trigonellin, synthesis,

96, 189


Pyridoxal fluorometric assay based on reductive amination

with methyl anthranilate and sodium cyanoborohydride, 96, 426

Pyridoxal phosphate functional site on purified tyrosine aminotrans-

ferase (rat liver), 95, 188 Pyrimidine

base, radiomettic assay for 5’-nucleotidase, 95, 497 dimers, DEAE disk assay for photoreactivation

in [3H]DNA (Escherichia cob), 97, 376 Pyrogallol

analysis of o-tocopherol in liver employing direct attack on tissue with alcoholic alkali (ovine), 97, 264

Pyrophosphate inorganic, analysis utilizing enzyme ATP sul-

furylase, 94, 117 radioactive, NAD+ analysis in picomole amounts,

97, 309 Pyrophosphorylase

-NAD, NAD+ analysis in picomole amounts, 97,309 Pyruvate

identity confirmation by enzymatic peak shift, 92,31 radioactive, decarboxylation by pyruvate dehydro-

genase, effect of ferricyanide (bovine), 98, 85 Pyruvate dehydrogenase

decarboxylation of [ I-i4C]pyruvate, effect of ferricyanide (bovine), 98, 85

rapid-mixing apparatus for continuous-flow studies, 97, 428

Pyruvate kinase chromatographic purification of nucleosidyl peptides

on dihydroxyboryl-substituted polyacrylamide and cellulose (rabbit), 99, 85

measurement of potassium by stimulation (plant material), 92, 370

R

Radiation inactivation of enzymes for size determination,

92, 2 Radioactivity

amino acid analysis, application to studies of protein metabolism in cultured cells, %, 45

background of immunoprecipitation, adsorbent for immune complexes used for protein synthesis assay in cells and cell-free translations (Staphy- lococcus aureus), 97, 24

counters, automatic sample-changer attachment, 96, 403

determination in ketone bodies, 92, 390 enzymatical microassay, determinations of 5-

phospho-D-ribose-cr-I-pyrophosphate pools (Salmonella typhimurium), 98, 264

guanidoacetate methyltransferase, assay, 95, 87 specific, synthesis and purification of [1-3ZP]fructose-

I$-bisphosphate, 96, 500

Radioassay enzymatic, dopamine and N-acetyldopamine (gas-

tropod& 96, 175 haptoglobin based on binding to hemoglobin (rab-

bit), 93, 355 in vitro protein synthesis by tibroblasts, 96, 139 isoelectric focusing study of plasma membrane actin

(chick fibroblasts), 95, 236 multiple isotopes using color negative films, 93, 189

Radiochemistry assay for N5-formytetrahydrofolic acid, 94, 483 measurement of diamine oxidase activity in amniotic

fluid (human), 96, 411 synthesis of 25-hydroxy[26,27-sH]vitamin D3 with

high specific activity, 96, 481 Radiochromatography

assay for cholesterol epoxide hydrase, 94, 383 Radioimmunoassay

antibody to $X174 DNA.RNA hybrid (Staphylococ- cus aureus), 98, 74

antiviral agent BW248U [9-(2-hydroxyethoxymethyl)guanine], 98, 319

determination of cyclooxygenase products and prostaglandin metabolites, 93, 339

hapten-antibody reaction, vinblastine and vincristine (Vinca rosea), 95, 214

immunoglobulin E, use of 12SI-labeled protein A (human), 93, 207

iodinating collagens to high specific activity with N- hydroxysuccinimide ester of 1Z51-labeled p- hydroxyphenyl propionic acid, 96, 489

liver metallothionein, labeled zinc thionein (rat), 98, 89

N’nitrosonomicotine, 95, 387 rapid technique for semiquantitative mass screening

of haptens, 92, 147 release, determination of chorionic somatomammo-

tropin plasma levels (human), 99, 121 renin and substrate concentrations in serum (hu-

man), 94, 125 solid phase, for lipoprotein lipase (chicken), 94, 346 vasoactive intestinal polypeptide (porcine), 97, 286

Radioimmunology screening method for specific membrane proteins

(Escherichia coli), 97, 153 Radioiodination

lactoperoxidase, of protein mixtures for electrophoretic analysis, 95, 568

peptide hormones containing tryptophan, 92, 46 preferential procedure for small amounts of protein

in presence of excess lipids, 99, 92 protein, comparison of techniques by tryptic pep-

tide mapping, 96, 84 Radioisotopes

assay, significance of sodium hydroxide in determination of protein, 100, 98

modified assay, measuring glutamine synthetase activity in tissue extracts (mouse), 94, 82


Radiometry assay for S-nucleotidase, 95,497 determination of L-canaline using [2-14C] acetone as

a reagent, 92, 265 one-step method for analysis of phenylethanol-

amine-N-methyltransferase in adrenal chromaf- fm cells (bovine), 99, 281

unified assay of enzymes of glutamate metabolism, 96, 24

assay for epidermal growth factor (human, rat), 93, 286

Radiorespirometry continuous monitoring of embryo development

(chicken), 98, 375 Reagents

storage and delivery using soluble hydrocolloid films, 95, 549

Receptors androgen, separation from testosterone-binding

globulin in human tissue, 92, 164 -binding assay, application of miniature ultracen-

trifuge, 96, 395 capacity, estimation for cyclic AMP-dependent

protein kinases, 98, 310 steroid receptor assay for quantitation of la,25-di-

hydroxyvitamin Q-receptor complexes (chicken), 92, 314

use of immobilized estradiol antiserum, 94, 278 Reduction

cleavage of folates, critical appraisal, 93, 91 Regression

analysis of simulated experimental biological data, 94, 210

Renin angiotensin I, radioimmunoassay of (human serum),

94, 125 Resins

cation-exchange, multiplicity of lipoxygenase- 1 demonstrated in chromatographic studies on protein (soybean), 95, 371

Respiration radiorespirometer for continuous monitoring of

embryo development (chicken), 98, 375 systems utilizing oxygenated leghomoglobin and

myoglobin as sources of free dissolved O2 at low concentrations (R&o&m), 96, 165

techniques for biochemical measurements, 95, 416 Restriction endonucleases

analysis of nucleotide sequence of DNA (review). 98, 243

cleavage of eluted DNA, recovery of intact high molecular weight DNA after electrophoretic separation, 98, 305

electrophoretic measurement of DNA length, log, 319

HpoII, hybridization method for quantitation of replicating polyoma DNA sequences (Haemo- philus parainfluenzae), 96, 378

PalI, purification (Provide&a afcalifaciens), 99,207

preparative gel electrophoresis apparatus for large- scale separations, 100, 304

Retina glycoprotein purification on high-capacity lectin af-

finity column (wheat germ), 97, 423 Retinaloximes

syn- and anti-isomers, identification and characterization, 99, 304

Retinoic acid blood, demonstration in normal physiological condi-

tions (human), 98, 402 Retinylphosphate

stimulation of mannosylretinylphosphate, chromatographic separation from dolichylmannosylphosphate (rat), 97, 298

Ribohomopolymers preparation in defined-length, 95, 171

Ribonuclease A pancreatic, Folate-Sepharose column as affinity

absorbent for protein fractionation (bovine), 98, 184

Ribonucleotides cyclic, practical aspects of reversed-phase liquid

chromatography applied to biochemical and biomedical research (review), 99, 1

Ribosomal proteins identification by gel electrophoresis (Escherichia

co/i), 92, 174 quantitative comparative analysis of complex two-

dimensional electropherograms, 97, 210 tracking marker for gel electrophoresis, 92, 203

Ribosomes eukaryotic dissociation factor, detection of (wheat

germ), 93, 238 free and membrane bound, visualization and quan-

tification of total complement (rat liver), 92, 305

procedure for preparation of large amounts of 50 S subunits (Escherichia coli), 99, 332

protein, quantitative comparative analysis of complex two-dimensional electropherograms, 97, 210

purification of cytoplasm elongation factor 2 and organellar elongation factor G (eukaryote), 99, 434

RNA labeled on methyl groups, purification (plant), loo, 70

RNA analytical and preparative gel electrophoresis,

98, 358 associated with intracistemal type A-particles, isola-

tion after cell disruption by nitrogen cavitation (mouse), 97, 85

base composition of nanogram quantities, 93, 26 chromatographic removal of DNA on acetylated


N-[N’-(m-dihydroxylborylphenyl)succinamyl]- amino-ethyl cellulose, 108, 174

DNA hybrid, radioimmunoassay of antibody to 4X174 (Staphylococcus aureus), 98, 74

electrophoretic fractionation by partial denaturation with methylmercuric hydroxide, 99, 200

electrophoretic selection and fractionation of poly(A)-containing RNA and poly(A), 92, 280

eukaryotic processing, relating long-range base pairing (simian virus 40), 95, 108

fluorimetric determination using ethidium dimer, 94, 259

high molecular weight fluorescent labeling, 93, 399 recovery after electrophoretic separation in low

gelling temperature agarose gels, 98, 305 homopolymer preparation, 95, 171 inhibition of nuclease contaminant in alkaline phos-

phatase (Escherichia coli), 95, 458 interference in quantitative DNA assay using adria-

mycin (bovine), 95, 559 interpretation of nitrocellulose filter assay of protein-

nucleic acid binding, 93, 204 isolation combining sodium dodecyl sulfate, pro-

tease K, sodium perchlorate, and isopropanol precipitation, 98, 116

messenger (mRNA) determination of 7-methylguanosine-containing 5’-

termini by NaB[3H]4 labeling and chromatography (rabbit), 98, 102

electrophoretic determination of size distribution (HeLa cell, mouse), 99, 146

preparation from immature erythrocytes (chicken), 98,464

nuclear electrophoretic determination of size distribution

(HeLa cell, mouse), 99, 146 preparation from carcinogen-treated embryo fibro-

blasts (hamster), 97, 410 poly(A), size determination of sequences in barley

stripe mosaic virus, 99, 450 preparative fractionation by agarose gel electro-

phoresis, 95, 174 preparative polyacrylamide gel electrophoresis

(Escherichia co/i), 93, 299 ribosomal (rRNA)

fingerprinting, gradient thin-layer chromatography of oligonucleotides as alternative to homochromatography, 98, 346

purification when labeled on methyl groups (plant), loo, 70

sequencing application of high-voltage polyacrylamide gel

electrophoresis, 100, 129 labeling procedures (rabbit), 93, 321

thiol-containing RNA, interaction with cellulose mercurial, 92, 324

transfer (tRNA) amino acid pools, protein metabolism in cultured

cells, 96, 45 -aminoacyl, column separation and fractionation,

94, 48 -aminoacyl, -GTP-elongation factor Tu complex,

quantitative assay for formation of, 92, 123 Sepharose 4B column chromatographic fractiona-

tion (Escherichia cob), 93, 248 simultaneous measurement of charging levels of

multiple aminoacyl-tRNAs, 99, 372 4-thiouridine sulfurtransferase, filter paper assay

for measurement of activity (‘Escherichia cob), 98, 146

tumor viruses, electrophoretic simultaneous isolation of major viral proteins, 98, 329

undegraded, detergent-citric acid technique for isolating nuclear and cytoplasmic fractions (Xenopus laevis), 108, 166

RNA polymerase II DNA-dependent, concentration of proteins using

carboxymethyldextran, 98, 353 RNase

activity, detection by positive zymogram method, %,464

pancreatic, determination of activity in serum (human), 188, 233

rapid high-yield isolation method for nuclear envelope ghosts (Tetrahymena pyriformis), 100, 282

Rubidium gelatin films analyzed by secondary ion mass

spectrometry, 92, 213 Rubidium chloride

gradient, function, initial density related to equilibrium density, 94, 193

Saccharamyces cerevisiae preparation of phosphoglycerate kinase using

cibacron blue-Sepharose 48 pseudoaftinity chromatography, 93, 46

Saccharides cellulase activity determined by gas-liquid chroma-

tography (ovine), 92, 270 Salts


Saxitoxin assay for tetrodotoxin binding to detergent extracts

of excitable tissues (Electrophorus electricus),

99, 72 Scintillation counting

tryptophan determination in proteins, 3-diazonium- 1,2,4-triazole reaction, 99, 464

Secretion


simultaneous measurement with aggregation, oxygen uptake, proton production, and intracellular metabolites in platelet suspension, 97, 387

Sedimentation

bilirubin, determination of total and free concentrations based on static fluorescence quenching of dansyl serum albumin (bovine, human), 96, 364

equilibrium, calculated molecular weight of proteins in high ionic strengths, contribution of apparent isopotential specific volume (Homarus americanus), 98,481

fractional, nonsynchronous flow-through coil planet centrifuge, 94, 249

Selenium

determination of ribonuclease (pancreatic) activity (human), 100, 233

enzymatic microdetermination of free fatty acids (human, rabbit), 98, 341

microassay for analysis of sulfate (human, mouse), loo, 339

effect on determination of mercury in tissues (mouse, rabbit), 98, 287

Sephadex

two-cross immunodiffusion for determination of precipitating titers and diffusion coefficients of immunoglobin, 93, 103

Shock Adogen 464 coated, separation of aminoacyl-tRNAs,

94, 48 -copper, separation of peptides from a-amino acids

(cheese, yeast), 97, 367 Sepharose

-dihydrofolate affinity column, purification of guanosine triphosphate cyclohydrolase I and dihydrofolate reductase, 100, 122

-heparin, potentiometric titration determination of heparin content, 95, 449

removal of concanavalin A from a- I-antitrypsin, 93, 180

osmotic, extraction of polysomes without use of detergent (Escherichia co/i), 99, 454

thermal, precedure for preparation of large amounts of 50 S ribosomal subunits (Escherichia co/i), 99, 332

Sialic acid erythrocyte surface residues, calorimetric deter-

mination, 97, 346 interference with thiobarbituric acid method for

measuring 3-deoxyoctulosonic acid, 100, 118

-N,N’,N”-triacetylchitotriose matrix, purilfication of potatoe lectin by affinity chromatography, 93, 88

Sepharose 4B

Sialoglycoconjugate quantitation of immobilization on insoluble supports,

varying ligand concentration, 100, 221 Siderophores

afhnity chromatography of albumin on bromosulfophthalein-glutathione conjugate covalently linked to (rat plasma), 92, 290

-cibacron blue pseudoaffinity chromatography used for preparation of phosphoglycerate kinase from Saccharomyces cerevisiae, 93, 46

-thiol, activated, purification of procollagen type II, 94, 253

catechol, chromatographic separation (Micrococcus denitrificans, Klebsiella aerogenes. Bacillus subtilis), 95, 364

Silicic acid column separation of gangliosides from lipids,

94, 335 Silicon

spurious peaks, in electron microprobe X-ray spectra of biological specimens (review), 94, 100

Silver -tridacnin affinity chromatography, isolation of

arabinogalactan, 92, 41 Sepharose 6B

stain for detecting proteins and peptides in polyacrylamide gels, 98, 23 1

Simian virus 40 purification of glyoxalase I from human erythrocytes

by afhnity chromatography and separation, 92, 390

-infected cells, fluorometric assay for nick-closing enzymes from (simian), 93, 346

relating long-range base pairing on single-stranded DNA and eukaryotic RNA processing, 95, 108

Sindbus virus Serine palmitoyltransferase

radioenzymatic assay for dopamine and N-acetyldopamine (gastropod& 96, 175

Serine proteases merits of chromophoric and fluorogenic leaving

groups, substrates for leukocyte and pancreatic elastase (human, porcine), 99, 53

Serotonin gas chromatography-mass fragmentography analysis

(rat brain), 92, 233 Serum

albumin, chromatographic study of binding of fatty acids and drugs (bovine), 99, 352

electrophoretic separation of glycopeptides, 97,438 Sodium

channel, assay for tetrodotoxin binding to detergent extracts of excitable tissues (Electrophorus electricus), 99, 72

substrate, fluorimetric assay of neuraminidase with (4 - methylumbelliferyl- a - D - N - acetylneuramin - ate), 94, 287

Sodium bicarbonate metabolism of ‘3C-compounds (rat), 96, 64

Sodium borotritiide


labeling, determination of 7-methylguanosine-con- taming .5’-termini of mRNA (rabbit), 98, 102

Sodium cyanoborohydride reductive amination of pyridoxal and its 5’-phos-

phate, fluorometric assay, 96, 426 Sodium desoxycholate

effect of extraction of myocardial creatine phosphokinase and protein (rat), 98, 278

Sodium dodecyl sulfate effect of detergents on antibody-antigen interac-

tion, 98, 445 electrophoretic mobility of carbamylated proteins,

93, 312 gel bands, protein fingerprinting, 97, 382 isoelectric-focusing slab gels, two-dimensional elec-

trophoresis of small-molecular-weight proteins, 99, 33

isolation of RNA, 98, 116 -mercaptoethanol-Tris electrophoresis buffer, quan-

titation of solubilized proteins, 100, 64 micellar and monomeric behavior with reference to

sodium dodecyl sulfate-protein complexes, 92, 243

micromethod removal from proteins by ion-pair extraction, 93, 1.53

molecular weight estimates from measurements of electrophoretic mobilities of proteins, 97, 210

polyacrylamide gel electrophoresis effect of composition of preparations on renatura-

tion of enzymes, 188, 357 removal of Triton X-106 from protein solution,

97, 116 simultaneous isolation of major viral proteins, 98,

329 two-dimensional separation of cellular proteins,

93, 329 -polyacrylamide gels

extraction of proteins and peptides, 96, 352 properties crosslinked with N, N ‘-diallytartardi-

amide, macromolecules isolated, 93, 275 recovery of functional proteins, 99, 170 recovery of proteins, 94, 274, 97, 229 visualization of protein bands, 93, 257

protein-bound, gas chromatography determination, 92, 444

proteins solubilized from densonucleosis virus, purification and peptide mapping of proteins (Galleria mellonella), 99, 97

removal of concanavalin A from a-l-antitrypsin, 93, 180

separation of DNA segments from protein using glass filters, 93, 158

-solubilized proteins, tissue-specific code based on, 92, 193

use of Triton X-100 to overcome fructosyltransferase inhibition, 97, 173

Sodium hydroxide effect upon protein binding to filter paper disks

and significance in determination of protein in calorimetric and radioisotopic assay, 108, 98

Sodium perchlorate isolation of RNA, 98, 116

Sodium salicylate fluorographic detection of radioactivity, 98, 132

Sodium tetraborate anhydrous, modified carbazole method for hexuronic

acid quantitation, 93, 295 Somatomammotropin

chorionic plasma levels, determination by release radioimmunoassay (human), 99, 121

Sonication isolation of intact mitochondria from liver cells

(rat), 93, 200 Spectrin

high-molecular-weight bands, separation by preparative slab gel electrophoresis, 92, 130, erratum, 95, 587

Spectroelectrochemistry small-volume cell constructed and characterized,

93, 366 Spectrofluorometry

chromatographic analysis of thiamine and its phosphate esters after conversion to highly fluorescent thiochrome derivatives, 97, 191

determination of free and endogenous tryptophan (Escherichia coli), 93, 390

front-face fluorometry of liquid samples, 94, 15 laser, for studies of cultured cells, 92, 74

Spectrometry apparatus

computerization, simultaneous measurement of fluorescence and phosphorescence using synchronously gated photon counters, 97, 158

fluorescence, coupled with automatic titration device, 94, 65

stainless-steel syringes for stopped-flow kinetics, 93, 407

atomization atomic absorption, application to trace metal analysis in silkworm eggs and hatched worms, 94, 386

measurement of haptoglobin concentration in plasma and other fluids (human), 99, 421

vaporization characteristics of metal chlorides, nitrates and sulfates, 95, 8

Spectrophotometry anaerobic, reaction kinetics study, 93, 380 apparatus

aminco-chance dual wavelength split-beam recording, use limitations and data interpretation from, 94, 450

cuvette, oxygen electrode system and microstirrer, 93, 386

differentiator for conversion into coagulometer and its application for quantitation of venom procoagulants (Crotalus horridus horridus. Agkistrodon contortrix mokasen), 97, 418

Gilford, precise temperature control, 93, 167 chromatographic-spectrometric estimation of de- laser, computerization which permits automated rived aldehydes with reference to ceramides of

operation of instrument for signal averaging, plasma (human), 97, 323 96, 456 Spores

microstirrer for insertion beneath cuvettes, 93,386 microfuge tube technique for disrupting micro- modified cuvette holder for temperature control, quantities (Ceratocystis ulmi), 100, 240

93, 167 Stabilization purpose and technique of the Keston polaramic of enzymes by immobilization (review), 93, 1

attachment to the Beckman DU spectro- Staining, see also specijc stains and dyes photometer, 92, 55 acridine orange, fluorescence used by fluorimeter for

small-volume cuvette for high-resolution thermal assay of glycosaminoglycans, 94, 378 denaturation of nucleic acids, 97, 136 binding estimation of protein concentration in crude

assay for L-aspartate:2-oxoglutarate aminotrans- cell and tissue extracts (rat), 94, 160 ferase, 100, 20 biologically active stains, intercalating fluorescent;

assay of phenolphthalein in biological fluids, 95, 465 electrophoretic determination of relative bind- assay with turbidity correction of sized immuno- ing constants, 95, 329

globulin G aggregates, 99, 346 cytochemical, peroxidase using 3,3’,.5,5’-tetramethyl- atomic absorption, estimation of proteins in colored benzidine dihydrochloride, 98, 388

or turbid solutions, 100, 92 dansylcadaverine, for transamidating enzymes, continuous assay for angiotensin converting en- 93, 453

zyme. 95, 540 direct visualization of collagens and procollagens continuous assay for cyclic 3’,5’-nucleotide phos- in polyacrylamide gels, 97, 359

phodiesterase, 97, 239 5,5’-dithiobis(2-nitrobenzoic acid), testing purity of coupled optical assay for determination of adenine phosphotransacetylase (Clostricium kluyveri),

in mixtures, 98, 213 95, 2 determination of ascorbic acid, 96, 1 extraction of proteins and peptides from Coomassie determination of cresolase activity of epidermis blue-stained sodium dodecyl sulfate-poly-

tyrosinase (Rana esculenta ridibunda), 95, 433 acrylamide gels, 96, 352 determination of lipid hydroperoxides, 99, 249 fluorescent activity of transamidating enzymes, determination of myoglobin in cardiac and skeletal 93, 445

muscle, 92, 473 fluorescent lectins, glycoproteins in polyacrylamide determination of tetraphenylboron and tetra- and agarose gels, 96, 208

phenylarsonium with ethidium bromide, 92,462 immunodiffusion, activity stain for histidinol de- direct assay for 5aminolevulinic acid dehydratase, hydrogenase, 92, 189

97, 277 improved dye uptake by glycosaminoglycans, 93,60 estimation of arginine decarboxylase (oat seedlings), properties of amido black lOB, Coomassie blue Rand

92, 331 G, and fast green FCF as proteins stains and estimation of glycosaminoglycan concentrations, dye impurities and their effects on protein-dye

99, 183 binding within gels, 96, 263 identification of dehydroascorbic acid from blood of Sirius Supra red F3BA, quantitative estimation of

diabetic as 2,4-dinitrophenylhydrazone deriva- collagen based on binding, 94, 96 tive (human), 98, 368 tannic acid, of enzymes in polyacrylamide gel

new chymotrypsin substrate synthesized, succinyl- electrophoresis, 95, 575 Ala-Ala-Pro-Phe-p-nitroanilide, 99, 316 visualization of egg yolk proteins after isoelectric

quantitative estimation of collagen based on bind- focusing during two-dimensional gel electro- ing of Sirius Supra red F3BA, 94, 96 phoresis (Smittia spec.), 98, 81

ultraviolet, measurement and calibration of peptide Stannous chloride group hydrogen-deuterium exchange, 92, 517 reducing agent, effect of selenium on determination

Spectroscopy of mercury in tissues (mouse, rabbit), 98, 287 emission, simultaneous measurement of fluores- Staphylococcus aureus

cence and phosphorescence using synchro- adsorbent for immune complexes, assay for specific nously gated photon counters, 97, 158 protein synthesis in cells and cell-free transla-

Spermidine tions, 97, 24 estimation of turnover (Anacystis nidulans), 95, 73 Steroids

Spheroplasts enzymatic analysis of hydroxysteroid dehydro- extraction of polysomes without use of detergent genases (Pseudomonas), 95, 286

(Escherichia co/i), 99, 454 enzymatic sulfation, preparation of 3’-phospho- Sphingolipids adenosine-5’-phosphosulfate, 96, 34



identification in biological matrices by mass- analyzed ion kinetic energy spectrometry, 96, 104

receptor, quantitation of la,25dihydroxyvitamin D,-receptor complex from intestinal mucosa (chicken), 92, 314

Sterol epoxide hydrase radiochromatographic assay, 94, 383

Stomach reticulum, cardiac sarcoplasmic reticulum isolation

from heart (bovine), 99, 41 Strontium

gelatin films analyzed by secondary ion mass spectrometry, 92, 213

Succinyl coenzyme A enzymatic method for measurement of GTP and

GDP in biological samples, 95, 512 spectrophotometric cycling assay of tissue levels

(mouse), 97, 103 Sucrose

gradient, electrophoretic mobility of membrane fragments, 92, 228

Sucrose phosphate synthetase ion-exchange assay in plant homogenates, 93, 196

Sucrose synthetase ion-exchange assay in plant homogenates, 93, 1%

Sugars interfering effect on Lowry method protein assay

(review), 108, 201 neutral

analysis as glycamines using amino acid analyzer, 97, 166

factors affecting stability, hydrolysis, reduction, acetylation, quantitation, and identification from vegetable fiber preparations, 96, 282

quantitation from differential culture media chromatographically, 99, 365

Sulfates alkyl, chromatographic analysis, 100, 357 metal, vaporization characteristics, 95, 8 serum, microassay for analysis (human, mouse),

loo, 339 Sulfation

assay of sulfotransferases, 95, 82 Sulthydryl groups

calorimetric determination of content in whole blood and plasma, 93, 98

distribution of argininosuccinase (bovine brain, liver, kidney), 95, 139

Sulfonic acid trifluoromethane, deprotection and resin cleavage,

95, 305 Sulfotransferases

coenzyme preparation, %, 24 enzyme transferred 3’-phosphoadenosine-5’-phos-

phosulfate, fiber binding assay, 95, 82 Sulfur

amino acids, chromatography on single-column amino acid analyzer, 98, 293

labile, liberation from ferrodoxins by alkaline zinc reagent, 95, 452

Sulfuric acid detection of glycoproteins in polyacrylamide gel,

99,474 detection of phosphate esters in thin-layer chroma-

tography, 96, 6 Sulfoxides

reduction in peptides and proteins, 98, 36 Sulindac

computerized spectrometric assay in plasma (human), 95, 579

Superoxide systems utilizing oxygenated leghemoglobin and

myoglobin as sources of free dissolved 0, at low concentrations (Rhizobium), 96, 165

Syringes type 412 stainless steel, for stopped-flow kinetics,

93, 407

1

Taka-amylase A tannic acid staining and extraction of enzymes in

electrophoresis, 95, 575 Tannic acid

staining of enzymes in polyacrylamide gel electrophoresis, 95, 575

Target volume theory size determination of enzymes by radiation inactiva-

tion, 92, 2 Temperature

calorimetric assay of hormone- and metabolite- induced changes in steady-state heat production by tissue (Zea mays), 100, 77

control, Gilford Spectrophotometer, 93, 167 elutriation control, resolution of erythrocytes

(human), 98, 112 reaction, effects on fluorescence yields, 93, 174 reduction of methionine sulfoxide to methionine in

peptides and proteins, 98, 36 Testosterone

-binding globulin, separation of androgen receptor, 92, 164

Tetraalkyl-ammonium hydroxide organic tissue solubilizer, digestion of muscle biop-

sies for trace metal analysis (human), 98, 315 Tetraborate

concentration on Morgan-Elson color reaction, hexosamine determination, 98, 433

Tetrabutylammonium counterion in reversed-phase ion-pair liquid chro-

matography of acyl coenzyme A thioesters, 94, 417

Tetraethoxypropane radioactive, hydrolysis to malondialdehyde, 99,458

N,-(2’-TetrahydrofuryB-5-fluorouracil chromatographic determination in biological speci-

mens (mouse), 97, 232


3,3’,5,5’-Tetramethylbenzidine dihydrochloride quantitative determination of hemoglobin and cyto-

chemical staining for peroxidase, 98, 388 Tetramethylmelamine

chromatographic-spectrometric identification in plasma as hexamethylmelamine metabolite (mouse), 99, 441

Tetranitromethane preparation and characterization of chemically

modified plastocyanin, 95, 254 Tetraphenylarsonium

spectrophotometric determination with ethidium bromide, 92, 462

Tetraphenylboron spectrophotometric determination with ethidium

bromide, 92, 462 Tetrodotoxin

assay, binding to detergent extracts of excitable tissues (Electrophorus electricus), 99, 12

Thiamine chromatographic analysis after conversion to highly

fluorescent thiochrome derivatives, 97, 191 Thiamphenicol

chromatographic separation, 96, 215 Thiazolinone

extractions, sequential analysis of peptides by phenylisothiocyanate procedure for degradation, %, 469

Thin-layer chromatography analysis of carcinogenic polycyclic hydrocarbon

metabolism by position-sensing proportional counting, 99, 165

characterization of amino acids using acidic mobile phases, 100, 254

detection of phosphate esters, %, 6 determination of LY- and @-5-phospho-n-ribose-a-l-

pyrophosphate pools (Salmonella typhimurium), 98, 264

determination of lithogenic index of bile using dual- wavelength chromatoscanner, 100, 146

gradient, preparing fingerprints of RNA and isolation of oligonucletides on DEAE-cellulose (Escherichia cob), 98, 346

identification of dehydroascorbic acid from blood of diabetic as 2,4-dinitrophenylhydrazone derivative (human), 98, 368

identification of glutamine synthetase assay products, 94, 82

identity of fractions containing 3-methoxytyramine and 3,4-dihydroxyphenylacetic acid tissue extracts, 92, 82

isolation of gangliosides from small amounts of tissue, 94, 335

method to isolate labeled 5-phosphoribosyl-a-l- pyrophosphate, determination of cellular pools and assay of synthetase activity, 98, 254

separation and quantitation of radiolabeled cho- lestan-3/?,5a,&-epoxide, 94, 383

separation of glycoprotein-derived oligosaccharides, 93, 167

separation of thyroid hormones and their metabolites (simian), 93, 3 1

three-directional, detection of amino acids in urine, 97, 328

Thiobarbituric acid measuring 3-deoxyoctulosonic acid, sialic acid inter-

ference, 100, 118 -periodate assay for hyaluronate lyase, determina-

tion of hyaluronate, 96, 474 reaction, assay for lipid peroxides (rat), 95, 351

Thiobenzl benzyloxycarbonyl-L-Iysinate substrate for a sensitive calorimetric assay for

trypsin-like enzymes, 93, 223 Thioglycosides

polyacrylamide gel preparation containing, 95, 260 Thiohydantoins

isocratic resolution by chromatography, 95, 494 Thiols

effects on fluorescence yields, 93, 174 Sepharose 4B, activated, purification of procollagen

type II, 94, 253 use in reduction of dithiasuccinoyl amino protecting

groups, 95, 160 4-Thiouridine-tRNA sulfurtransferase

filter paper assay for measurement of activity (Escherichia coli), 98, 146

Thricodermin inhibition of protein synthesis, preparation of poly-

somes and mRNA from immature erythrocytes (chicken), 98, 464

Thymol detection of glycoproteins in polyacrylamide gel,

99, 474 Thyroid hormones

separation from metabolites by thin-layer and exchange chromatography (simian), 93, 31

Thyroid-stimulating hormone (TSH) fluorophotometric enzyme immunoassay (human),

96,419 Thyrotrophin

receptor purification, preparative flatbed electrofocusing in granulated gels with natural pH gradients, 97, 95

Tissue extract, estimation of protein concentration, 94, 160 extracts, nucleotides, microprocedure, 93, 227 extracts, radioisotopic assay for measuring gluta-

mine synthetase activity (mouse), 94, 82 Titer

precipitating, two-cross immunodiffusion in body fluids, 93, 103

Titrations assay of glycosaminoglycans using fluorimeter,

94, 378 chloride ion, determination of amino groups on

amino propyl glass, 94, 60 fluorescence


computer controlled apparatus, 94, 65 quenching, graphical correction procedure for in-

ner filter effect, 96, 448 potentiometric, determination of amino groups on

amino propyl glass, 94, 60 Tobacco acid pyrophosphatase

determination of 7-methylguanosine-containing 5’- termini of mRNA (rabbit), 98, 102

a-Tocopherol analysis in liver employing direct attack on tissue

with alcoholic alkali in presence of pyrogallol and hydroquinone (ovine), 97, 264

Toluene organic tissue solubilizer, digestion of muscle biop-

sies for trace metal analysis (human), 98, 315 Transferrin

binding studies, quantitation and differentiation of lectin-glycoprotein complexes by laser nephelometry (human), 96, 391

Transglutaminases fluorescent activity staining procedure, 93, 445 liver, two enzymatically active forms (guinea pig),

93,453 Transketolase

enzymatic method for analysis of D-erythrose Cphos- phate, 95, 250

Translation cell free, analytical and preparative gel electrophore-

sis of RNA, 98, 358 Trehalase

continuous coupled polarographic assay, 94, 360 N,N’,N”-Triacetylchitotriose

-Sepharose matrix, purification of potato lectin by affinity chromatography, 93, 88

Trialkylphospines use in reduction of dithiasuccinoyl amino protecting

group, 95, 160 Tricarboxylic acid

cycle metabolites, measurement with glycolytic acid in

single sample aliquots, 97, 394 micromethod for purification and quantification of

organic acids, 95, 3 11 Trichloro-s-triazine

isolation of protease inhibitor by affinity chromatography (human urine), 92, 383

Tridacnin -Sepharose 4B, affinity chromatography, isolation of

an arabinogalactan, 92, 41 Trifluoromethane sulfonic acid

deprotection and resin cleavage, solid-phase tuftsin synthesis, 95, 305

Trigonelline synthesis of I-methylnicotinic acid, %, 189

Trimethylamine in urine, gas-liquid chromatography analysis

(human), 94, 358 Trinitrobenzenesutfonic acid

method for monitoring amino acids and peptides in chromatographic column effluents, 96, 317

Trioses fluorometric determination with o-phenylphenol,

94, 1 Tritium

-containing polyacrylamide gel slices, one-step liquid scintillation counting method, 99, 161

electrophoretic labeling of protein on sodium dodecyl sulfate-polyacrylamide slab gels, 95, 359

-labeled chloramphenicol, chromatographic preparation, 96, 215

-labeled e&radio1 with estradiol receptor, immobilized antiestradiol antibody set in competition for, 94, 278

potassium borohydride galactose oxidase method for labeling pectic polysaccharides (grapefruit), 98, 429

Triton X-100 detecting foreign DNA in plasmids (Escherichi~

coli), 98, 60 removal from aqueous solution using amberlite

XAD-2, 92, 447 single extraction removal from isoamyl alcohol,

93, 139 use to overcome inhibition of fructosyltransferase

by SDS, 97, 173 Trypsin

assay using radioiodinated protein substrates (Xenopus laevis), 100, 352

assay with 3H-peptidyl anilides, 97, 269 inhibitors, quantitative assays using immobilized

trypsin, 98, 438 -like proteases, immobilized anhydrotrypsin as bio-

specific affinity adsorbent for peptides (bovine), 98, 198

-like protease substrate, synthesization of thiobenzl benzyloxycarbonyl+lypsinate, 93, 223

purification, porous silica gel as bioadsorbent and biocatalyst, 98, 238

Tryptamine gas chromatography-mass fragmentography analy-

sis (rat brain), 92, 233 Tryptophan

free and endogenous, determination (Escherichia coli), 93, 390

gas chromatography-mass fragmentography analysis (rat brain), 92, 233

manually operated and autoanalyzer determination on the formation of norharman (plasma), 92,338

scintillation method for determination in proteins, 3-diazonium-1,2,4-triazole reaction, 99, 464

Tubular fluorometric assay for urea, 98, 132

Tubulin assembly requirements, comparison of microtubule

protein and neuronal microtubules (bovine, hog), %, 225

-microtubule equilibrium in cultured celis, biochemical determination, 96, 155

Tuftsin


solid-phase synthesis, deprotection and resin cleavage with trifluoromethane sulfonic acid, 95, 305

Tumors carcinogen-induced lymphoma, hydroxylapatite

batch assay for quantitation of cellular DNA damage (mouse), 97, 77

mammary, association constant and estrogen receptor concentration (mouse), 94, 29

Tyrosinase epidermis, spectrophotometric determination of

cresolase activity (Rana esculenta ridibundu), 95, 433

Tyrosine aminotransferase cell-free synthesis, assay using adsorbent for im-

mune complexes (S@rylococcus aureus), 97, 24

chromatographic isolation and purification (rat), 95, 188

Tyrosine hydroxylase activity, fluorescence assay based on isolation of

enzymatically formed dopa (brain), 93, 82

U

UDP-glucuronyltransferase fluorometric assay using 3-hydroxybenzo(a)pyrene

as substrate, 98, 394 Ultracentrifugation

apparatus, application in receptor-binding assay, 96, 395

function of cesium chloride and rubidium chloride as gradients, initial density related to equilibrium density, 94, 193

sucrose gradient, isolation of myocardial subcellular fractions (canine), 96, 293

Ultrafiltration methodology, 95, 199

Ultraviolet -absorbing ion pairs, chromatographic determination

of free and conjugated bile acids, 100, 260 Unfolding

-refolding, enzyme stabilization by immobilization (review), 93, 1

Urea fluorometric assay in urine, plasma, and tubular

fluid, 98, 132 Urease

aluminium hydroxide system, immobilization for determination of enzyme structure, 100, 54

assay using ammonia electrode, 95, 483 Ureido

calorimetric analysis of compounds, 97, 42 1 Uric acid

chromatographic simultaneous analysis with hypoxanthine, xanthine, allopurinol, and oxipurinol, 98, 18

Uridine diphosphates, see UDP Uridine

nucleotides, specific characterization of trace amounts (hamster), 95, 245

Uridylyl adenosine substrate, positive zymogram method for detecting

RNase, 96, 464 Urine

analysis of prostaglandin E, (human, rat), 98, 455 assay for measurement of p-aminobenzoic acid in

oral pancreatic function test (human), 98, 13 determination offree y-carboxyglutamic acid, 98,219 fluorometric assay for urea, 98, 132 gas-liquid chromatography analysis of trimethyla-

mine in (human), 94, 358 isolation of protease inhibitor by affinity chroma-

tography (human), 92, 383 measurement of N5-formyltetrahydrofolate, 94, 483 measurement of histamine (rat), 94,425 3-methylhistidine, analysis by exchange chroma-

tography (human), 92, 294 phenylacetic acid excretion, mass fragmentography

(human), 99, 283 Urokinase

assay with “H-peptidyl anilides, 97, 269 Uranic acid

modified carbazole method for determination in vegetable fiber preparations, 96, 282

Uroporphyrin I and III octamethyl esters, chromatographic separa-

tion, 95, 444

V

Valyl-proline isolation of as direct measurement technique for

elastin, 92, 61 Vasopressin

chromatographic purification of neurophysins on methionyl-tryosyl-phenylalanyl-aminohexyl-agarose and measurement of ligand binding parameters, 97, 302

Venom micro determination of polyamines with oxidase

(snake), 92, 453 phosphodiesterase conversion of supercoiled DNA

to relaxed DNA (snake), 93, 346 procoagulants, differentiator for conversion of

spectrophotometer into coagulometer (Crotalus horridus horridus, Agkistrodon contortrix mokasen), 97, 418

Vinblastine radioimmunoassay of hapten-antibody reaction

(Vinca rosea), 95, 214 Vincristine

radioimmunoassay of hapten-antibody reaction (Vim-a rosea), 95, 214

Virions purification and characterization of tumor virus

glycoprotein (mouse), 94, 40 Vitamin A acid, see Retinoic acid


Vitamin C automated assay in plasma tissues (rat), 92, 510

Vitamin Ds radiochemical synthesis of 25-hydroxy[26,27-3H]-

vitamin D3 with high specific activity, 96, 481 Vitamins

steroid receptor assay quantitating la,25dihydroxy- vitamin Ds-receptor complex from intestinal mucosa (chicken), 92, 3 14

Vitellogenin chromatographic and precipitative isolation from

plasma (Xenopus luevis, vertebrates), 97, 145 Voltammetry

assay for aromatic L-amino acid decarboxylase activity by chromatography, 100, 160

cyclic, characterization of thin-layer spectroelectrochemical cell, 93, 366

W

Warfarin binding of fatty acids and drugs to immobilized

serum albumin, chromatographic study (bovine), 99, 352

Water sublimation, extraction and measurement of deuterium

oxide at tracer levels, 98, 208

X

Xanthine chromatographic simultaneous analysis with hy-

poxanthine, allopurinol, oxipurinol, and uric acid, 98, 18

Xanthine oxidase fluorimetric assay for adenosine, inosine, and hypo-

xanthine, 95, 377 simultaneous analysis of substrates, products, and

inhibitors chromatographically, 98, 18 X-ray spectra

biological specimens, sources of silicon peaks (review), 94, 100

Z

Zinc alkaline reagent, liberation of labile sulfur from

ferredoxins, 95, 452 chelate chromatography, purification of plasma (L*

macroglobulin and or proteinase inhibitor (human), 99,415

spectrophotometric assay for angiotensin converting enzymes, 95, 540

Zinc thionein labeled, radioimmunoassay of liver metallothionein

(rat), 98, 89

Documents

Cumulative subject index volumes 92–100