from diagnosis, the seeds of better health

ISOLATION and ENUMERATION of all urinary tract pathogensColonies are well isolated and easy to identify with real differentiating colours.Microbial enumeration with colourless background to optimise colony counting (for inoculation and interpretation, see back page).

DIRECT IDENTIFICATION CPS ID 3, NEW generation contains specific substrates of the enzymatic activities to be detected.

GREATER PRATICABILITY● E.coli: ß-glucuronidase (ß-GUR). No indole test required.

● Proteus: spontaneous colouration of colonies producing deaminase. No additional TDA test required.

● Enterococci: ß-glucosidase (ß-GLU).

E.coli

P.mirabilis

Enterococci

Pink to burgundy coloniesß-glucuronidase +ß-glucosidase -

E. coli

Light brownto dark brown coloniesß-glucuronidase - / ß-glucosidase -deaminase +

ProteusDetection of Indole

Indole + Indole -

Proteus indole +Morganella, Providencia

Proteus mirabilis

Enterococci Follow up identification

Direct examination

Turquoise coloniesß-glucuronidase -ß-glucosidase +

CocciBacilli

Klebsiella pneumoniae➡ Green to brownish-green

colonies : ß-GLU +➡ Direct examination : bacilli➡ Complete the identification

GREATER RELIABILITY AND IMPROVED PERFORMANCE * The NEW GENERATION of CPS ID 3 has increased :

● Nutrient capacity, mainly for staphylococci and yeasts.

● Sensitivity of detection and specificity of colouration for the main organisms usually found in urine.

● Identification of the bacterial group KESC (Klebsiella, Enterobacter, Serratia and Citrobacter)KESC => better colour intensity, due to increased ß-glucosidase activity.

● Orientation of identification towards Streptococcus agalactiae.

Streptococcus agalactiae➡ Usually violet colonies ➡ Presumption of

Streptococcus agalactiae ➡ Complete the identification

Mixed culture showing :E.coli, Proteus and Enteroccoci

Immediate identification with :

● Pink to burgundy : E.coli

● Light brown to dark brown : Proteus

● Turquoise : Enterococci

Mixed culture showing :E.coli and P. mirabilis

Mixed culture showing :E.coli and Enteroccoci

Mixed culture showing :K.pneumoniae

and Enteroccoci

Candida albicans➡ White colonies ➡ Direct examination : yeasts ➡ Complete the identification

IDENTIFICATION OF POLYMICROBIAL MIXTURES

Pseudomonas aeruginosa➡ Pigmented colonies ➡ Direct examination : mobile bacilli➡ Oxidase +➡ Complete the identification

Staphylococcus aureusImmediate identification with : ➡ Usually yellow colonies➡ Direct examination : cocci➡ Catalase +➡ Slidex Staph Plus

106 CFU/ml

104 CFU/ml

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www.biomerieux.com

Microbial enumeration :● Inoculation with a 10 µl calibrated loop.

● Interpretation of bacteriuria :< 104 CFU/ml : probable contaminationbetween 104 and 105 CFU/ml : equivocal result, repeat test on a new specimen> 105 CFU/ml : probable infection

● Compare the density of the colonies detected with that illustrated below(CFU = Colony forming Unit) :

CPS ID 3 : Ref. 43 541 : kit of 20 plates - Ref. 43 549 : kit of 100 plates

ID Indole TDA : Ref. 56 541 : 200 testsID color Catalase : Ref. 55 561 : 100 testsOxidase Reagent : Ref. 55 635 : 50 ampulesRAPIDEC STAPH : Ref. 03 300 : 10 strips x 3 testsSlidex® Staph Plus : Ref. 73 115 : kit of 50 tests - Ref. 73 116 : kit of 250 tests

bioMérieux offers a wide choice of media, including a comprehensive range of ready-to-use chromogenic media. Consult your local bioMérieux representative for further information and availability.

* For more details see technical sheet

103 CFU/ml

105 CFU/ml