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Wide-Area All-Optical Neurophysiology in Acute Brain Slicessmaller surface area. After cells reached 80% confluency, cells were switched to 16 ml pre-warmed DMEM without FBS for 1–2
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Influence of Cell Confluency on the Expression of the α4 ...file.scirp.org/pdf/ABC_2015033015164214.pdf · J.-M. Bourget et al. 74 1. Introduction The retinal pigment epithelium
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Microfluidic Concentric Gradient Generator Design for High … · cell channels from each other. Cell lines are perfused into each concentric channel, seeded to the desired confluency
Cell Imaging, Cell-based Assays, Spheroid Imaging, Stem ...€¦ · Cells propagated in the flasks for seven days while the cells reached a confluency of 80%. A 600 µL volume of
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Transfection Methods Overview - bio-rad.com · Confluency and Growth Phase Cells should be transfected at 40–80% confluency (cell type dependent) – Too few cells cause cell cultures
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Research Paper Generation of Stable Induced Pluripotent ...FUW-M2rtTA (Addgene) were used to reprogram the zFFs and zSEFs. After reaching 70% confluency, the 293T cells were digested
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The Complete Guide To Optimizing IMMUNOFLUORESCENCE … · 10 The Complete Guide To Optimizing Immunofluorescence Staining 1. When cells have reached the desired cell confluency,
RESEARCH Open Access Pro-inflammatory cytokines derived ...SK-N-SH cell line grown to 90% confluency in 6-well plates (6 × 105 cells/well) were infected with WNV (NY99) at the multiplicity
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Protein-disulfideIsomeraseDisplacestheCholeraToxinA1 ... · CTA1 Transfection Assays—Cells seeded to 80% confluency in 6-well plates were transfected with pcDNA3.1/ssCTA1 (35) usingLipofectamine(Invitrogen)accordingaspermanufactur-er’s
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Immortalization of primaryhuman smooth musclecells · controls and continue to divide withoutgrc confluency. In addition, they survive in medium(0.5%fetal bovine serum). Atappr passage
