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SOC Buffer in 1 Litre Tryptone 20g (2%) Yeast Extract 5g (0.5%) Sodium Chloride 0.5g (0.05%) Potassium Chloride 0.186g (0.0186%) Water 1L 1 M MgCl 2 2mL 1 M MgSO 4 2mL 1M of D- Glucose 4mL SOB Buffer in 1 Litre Tryptone 20g (2%) Yeast Extract 5g (0.5%) Sodium Chloride 0.5g (0.05%) Potassium Chloride 0.186g (0.0186%) Water 1L 1 M MgCl 2 2mL 1 M MgSO 4 2mL

Competent Cells Preparation

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Competent Cells Preparation

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SOC Buffer in 1 LitreTryptone 20g (2%)Yeast Extract5g(0.5%)Sodium Chloride 0.5g(0.05%)Potassium Chloride0.186g(0.0186%)Water1L1 M MgCl22mL1 M MgSO42mL1M of D- Glucose 4mL

SOB Buffer in 1 LitreTryptone 20g (2%)Yeast Extract5g(0.5%)Sodium Chloride 0.5g(0.05%)Potassium Chloride0.186g(0.0186%)Water1L1 M MgCl22mL1 M MgSO42mL


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