COMPENSATORY GROWTH OF TWO BROILER … · COMPENSATORY GROWTH OF TWO BROILER GENOTYPES FOLLOWING THERMAL CONDITIONING AND CRUDE ... 21 days according to the ... during days 3-4 of

8

JAgricampEnvSciDamUnivEgypt Vol9 (3) 2010

COMPENSATORY GROWTH OF TWO BROILER

GENOTYPES FOLLOWING THERMAL

CONDITIONING AND CRUDE PROTEIN REGIMEN UP

TO DAY 21 OF LIFE

W El-TAHWAY1 Y A ATTIA

1 S E AGGREY

2 and W S HABASHY

1

1Department of Animal and Poultry Production Faculty of Agriculture Damanhour

University 2 Poultry Science Department University of Georgia Athens GA 30602-2772

saggreyugaedu

ABSTRACT

This study aimed to investigate the compensatory

growth of two broiler genotypes following exposure to

thermal conditioning (TC) and protein regimen (PR) during

days 1-21 of age Broiler males (n=256) from Hubbard (Hub)

and equal number from Arbor Acres (AA) strains were

randomly distributed in factorial experimental design

among 8 treatment groups (2 protein regimen by 2 thermal

conditions (group of chicks exposed to 373ordm C and 70 RH

for 24 h at day 3 and returned to normal TC and unexposed

control group C (33 ordm C and 45 RH) by two strains of

broilers (Hub and AA) Protein regimen during the

experimental starter period was high CP regimen being 24

(high) and standard protein regimen ie 22 (standard)

Exposing broilers to TC at day 3 of age resulted in

significantly lower growth and worse FCR during days 3-4

of age than the control group However catch-up growth

and feed utilization was shown by day 21 of age T3 at day 21

of age was significantly lower of TC group than the C group

Growth was higher of high PR group but FCR was

similar between high PR and low PR during days 3-4 and 1-

21 of age Plasma albumin glucose MDA basophils and

phagocyte index (PI) were significantly higher of high PR

but plasma globulin albumin ratio was lower

Strain of broilers had a significant effect on growth

during days 3-4 and 1-21 of age WBCs lymphocyte

8


basophils and esinophil showing AA chicks had superior

productive traits 1-21 WBCs and esinophil than Hub

chicks but had lower globulin to albumin ratio basophils

and heterophils

A significant interaction between strain TC and

protein regimen was observed on BWG during days 3-4 and

1-21 of age FCR during days 1-21 of age and phagocytes

activity (PA)

It could be concluded that broilers exposed to TC and

fed standard PR exhibited compensatory growth suggesting

that TC and standard PR may be suitable management tool

to improve chickens tolerance to heat stress late in life

particularly in high temperature regions

Keywords Thermal conditions broiler genotypes protein regimen growth

INTRODUCTION

The global mean surface temperature increased by 08 and 17 ordm

C during the 19th

and 20th

centuries and expected to increase by 06 to

25ordm C during the next 50 years

(httpwwwncdcnoaagovoaclimateglobalwarminghtml)

Increasing ambient temperatures decreases survival and productive

traits (Dale and Fuller 1979 Teeter et al 1985 Deaton et al 1986

Attia et al 2006) The negative effect of heat stress on growth

performance of broilers may be related to the sensitivity of

commercial broilers to high temperature and can be attributed in part

to the inferior development of their cardiovascular and respiratory

system in comparison to their rapid growth potential (Yahav 2000)

The high growth of broilers is connected with high feed intake and

increasing metabolic heat production (Teeter 1994)

Appropriate combination of nutritional and management

strategies are essential for optimal broiler production during heat

stress Thermal conditioning (TC) at an early age has been proposed

by various authors (Arjona et al 1990 Yahav 2000 De Basilio et

al 2001) as a possible solution to reduce susceptibility of broilers to

heat stress however the ability of broilers to recover and show

compensatory growth is addressed (Yahav 2000)

97


Feed and protein intakes as well as protein synthesis and

breakdown were negatively affected by heat stress (Daghir 2008 Lin

et al 2006) and protein synthesis is more affected than protein

breakdown leading to reduce protein deposition that cannot be

restored by high dietary protein (Temim et al 2000) Furthermore

growth rate is suppressed by high levels of dietary protein (Cahaner et

al 1995) and may not are not necessarily increased (Attia et al

2011) under high ambient temperatures Feeding increased protein

could rather be more detrimental to the heat-stressed birds due to the

higher heat increment associated with protein metabolism besides

dietary proteinamino acids also affects protein turnover resulting in

more metabolic heat production (Musharaf and Latshaw 1999) This

study aimed to study compensatory growth of two strains of broilers

following thermal conditioning and crude protein regimen up to day

21 of life

MATERIALS AND METHODS

Birds and Design

A total of 256 chicks from Hubbard (Hub) strain and equal

number of Arbor Acres (AA) chicks at one-day of age were randomly

distributed keeping approximately equal initial body weight in

factorial experimental design among 8 treatment groups (2 protein

regimen by 2 thermal conditions by two strains of broilers)

The dietary CP treatments were 24 CP with 3098 kcal ME kg

from day 1 to 21 of age [High protein regimen] and 22 CP with

3099 kcal ME kg from day 1 to 21 of age [Standard protein regimen]

The composition of the experimental diets and their chemical analysis

are shown in Table 1 The experimental diets were formulated based

on NRC (1994) At day 3 chickens in each CP regimen were divided

into two groups in which one group was exposed to thermal

conditioning (TC) at 373ordm C and 70 RH for 24 h and returned to

control conditions (33 ordm C and 45 RH) condition The other was

unexposed Each treatment was replicated 4 times of 8 chicks each

Management

Chicks were housed in battery brooders from day 1 till 21 days

Feed and water were offered ad libitum Chicks were provided with

97


24 hr of light during day 1 to 3 of age and 23 1 light dark cycle

thereafter Chickens were vaccinated with Hitchiner + IB Influenza

H5 N2 and Gumboro at days 8 11 and 13 of age respectively

Data Collection

Birds were weighed (g) individually at days 1 3 4 and 21 of age

during the experimental period Feed intake was recorded at 3 4 and

21 days according to the replicate-feeding system followed in the

present work and the feed conversion ratio (FCR) was calculated

Blood hematological were determined using 8 samples per treatment

at day 21 of age Hemoglobin concentration (Hgb) was determined as

gdL by the cyanomethemoglobin procedure (Eilers 1967)

Heparinized blood was used for determination of packed cell volume

(PCV) using Wintrobe hematocrit tubes Blood samples were

centrifuged for 20 min at 4000 rpm then PCV values were obtained by

reading the PCV on the graduated hematocrit tubes Red blood cells

(RBCs) were counted on an Ao bright line hemocytometer using light

microscope at 400x magnification Blood samples were diluted 200

times with physiological saline solution before counting White blood

cells (WBCs) were counted on an Ao bright line hemocytometer

using a light microscope at 100x magnification after diluting the blood

samples 20 times with a dilution fluid (3ml glacial acetic acid + 97 ml

distilled water + Lushman stain) according to Hepler (1966)

Differential leukocyte counts were examined in each blood film by

using light microscope with 1000 x magnification power A thin blood

film was prepared by using a small drop of blood The blood film was

completely dried before staining using Giemsa stain The film was

washed in distilled water and dried The number of any individual

type of WBCs was expressed as a percentage of the total (Lucas and

Jamroz 1961) The method of Kawahara et al (1991) was used for

determination of phagocytes activity (PA) and phagocytes index (PI)

Eight blood samples per treatment were also collected in heparinized

tubes at day 21 of age for determination of some biochemical

constituents Plasma was obtained by centrifugation of blood at 1509

g for 20 minutes and was stored at -20 Co for further analyses Plasma

glucose concentration (microgdl) was measured by the method of Trinder

(1969) using commercial kits (Diamoned Diagnostics) Total protein

(gdl) was measured by Weichselbaum (1946) Albumin concentration

(gdl) was determined according to the method of Doumas et al

97


(1977) Globulin concentration (gdl) was estimated by subtraction of

albumin concentration from plasma total protein value according to

Coles (1974) Total plasma cholesterol (mgdl) was determined

according to the method of Watson (1960) The activity of alkaline

phosphatase (ALP) enzyme was assayed in plasma by the method of

Yan et al DGKC 1972) Total antioxidant capacity (micromoll) and

malondialdehyde (MDA micromolL) were determined according to

Koracevic et al (2001) and Richard et al (1992) respectively Plasma

T3 (nmolL) was determined according to the method of Young et al

(1975)

Statistical evaluation

Data were statistically analyzed using PROC ANOVA of

SASreg (SAS Institute 1996 Cary NC USA) Heat conditioning diet

and strains as well as all possible interaction were included in the

factorial model All the percentages were converted as log10 to

normalize data distribution Mean differences at P005 were tested

using the Tukey test (SAS 1996)

RESULTS AND DISCUSSION

Productive performance

Thermal conditioning significantly decreased growth and

impaired FCR of broilers by 403 and 478 respectively only

during days 3-4 of age (Table 2) In addition TC had a significant

negative effect (12) on feed intake of broilers during days 3-4 and 1-

21 of age This result is in agreement with (Yahav 2000) who found

that the negative effect on BWG was coincided with a decrease in feed

intake and short life span of broiler chicks Similarly Attia et al

(1995) and (Saleh et al 1996 Yahav and Plavnik 1999) showing

adoption and compensatory growth the phenomena that are known to

occur after exposing animals to stress Protein regimen and genotype

had a significant effect on growth during days 3-4 and 1- 21 of age

The obtained data confirmed those reported by (Attia et al 2001)

who showed that feeding high PR significantly increased BWG during

days 1-21 of age showing higher protein requirements to fulfill higher

growth during early growth phase On the other hand protein regimen

97


Table 1 Composition calculated and chemical analysis of the

experimental starter diets fed during days 1-21 of age

Ingredient Protein level

24 22

Yellow corn 49175 5528

Soybean meal (48) 38500 3300

Corn gluten meal 3000 300

Dicalcium phosphate 1800 183

Limestone 1300 135

NaCl 0400 040

Premix1 0300 030

DL-Methionine 0160 021

L-Lysine Hcl 0065 023

Vegetable oil 5300 440

Sand 0000 000

Total 100 100

Calculated2 and determined

3 chemical analysis

ME kcalkg 3098 3099

DM 8998 8683

CP 2 2408 2201

CP 3

2421 2266

Lysine 2

126 125

Methionine 2 055 057

SSA 2 093 093

Threonine 2 102 094

Arginine 2 169 154

Tryptophan 2

033 029

Calcium 2 101 102

Phosphorus available 2 048 048

Ash 3 682 684

1Vit+Min mixture provides (per kg diet) vitamin A (retinyl acetate) 24 mg vitamin E (dl--

tocopheryl acetate) 20 mg menadione 23 mg Vitamin D3 (cholecalciferol) 005 mg

riboflavin 55 mg calcium pantothenate 12 mg nicotinic acid 50 mg choline chloride 600

mg vitamin B12 10 microg vitamin B6 3 mg thiamine 3 mg folic acid 1 mg d-biotin 050 mg

Mn 80 mg Zn 60 mg Fe 35 mg Cu 8 mg Se 060 mg 2 Calculated according to NRC (1994) 3 Determined chemical composition according to (AOAC 1990)

97


Table 2 Main effect of thermal conditioning dietary protein regimen and broiler strain on body weight gain feed intake and feed conversion ratio of broiler chickens

Body weight gain g Treatments

Days 1-21 3-4 d Days

Thermal condition1

521 788b

TC

524 132a

C

NS 00001 P value

322 0315 SEM

Protein regimen1

527a

110a

High

517b

101b

Standard

003 0046 P value

322 0315 SEM

Strain1

507 b

112 a

Hub

537 a

984 b

AA

00001 0006 P value

322 0315 SEM

Feed intake g chick period

Thermal condition2

664b

190b

TC

690a

216a

C

0004 00011 P value

629 0544 SEM

Protein regimen2

681 200 High

673 206 Standard

NS NS P value

629 0544 SEM

Strain2

671 208 Hub

683 198 AA

NS NS P value

629 0544 SEM

Feed conversion ratio g feedg gain

Thermal condition2

128 235a

TC

131 159b

C

NS 00001 P value

97


Cont Table 2 Main effect of thermal conditioning dietary protein regimen and broiler strain on body weight gain feed intake and feed conversion ratio of broiler chickens

0013 0072 SEM

Protein regimen2

129 187 High

130 207 Standard

NS NS P value

0013 0072 SEM

Strain2

131 199 Hub

128 195 AA

NS NS P value

0 013 0072 SEM 1 Number of observation =256

2 Number of observation =32

abc Means within a column not sharing a common superscript are

significantly different Pgt 005 NS= Not significant TC=thermal conditioning C=Control

and genotype had no significant effect on FCR during days 1-21 of

age indicating that broilers chicks exhibited compensatory growth

There was a significant interaction between strain of broiler

TC and PR on BWG during days 3-4 and 1-21 of age (Table 3)

During days 3-4 of age under thermal conditions (C and TC) feeding

high PR significantly enhanced growth of only Hub broilers compared

to standard PR However high PR induced a detrimental effect on

growth of AA chicks under only TC During days 1-21 of age Hub

broilers responded similarly to different PR under C and TC however

high PR increased BWG of AA chicks under C but induced the

contrary effect under TC this indicated PR by strain interaction

Similarly Widyaratne and Drew (2011) indicated that low-protein

diets can support growth performance equal to high-protein diets In

the contrast there was no significant interaction in feed intake during

all tested periods (Table 3) There was a significant interaction in FCR

during days 1-21 of age FCR of the AA chicks exposed to control and

TC and fed high PR was significantly better FCR than Hub chicks on

the same treatments Broilers of both strains exposed to TC at day 3 of

age and fed standard PR had the best FCR during days 1-21 of age

Meanwhile under C condition feeding Hub standard PR and AA

98


99


chicks high PR resulted in the best FCR with later strain produced

significantly better FCR than the Hub one These results indicated that

the response of productive performance of broilers chicks to PR

depends on TC and broiler genotype

Biochemical constituents of blood plasma

Total plasma protein albumen globulin glucose alkaline

phosphatase cholesterol and total antioxidant capacity (TAC) at day

21 of age were not significantly affected (Table 4) However MDA at

day 21 of age was significantly higher of TC groups than that of C

These results are similar to those reported by Yahav and McMurtry

(2001) and Requena et al (2010) who indicated that the effect of TC

depends on the severity and length of heat exposure (Attia et al

2011) In addition TC lowered significantly plasma T3 and plasma

globulin-albumin ratio than the C group

Protein regimen significantly affected only plasma albumin

globulin and globulinndashalbumin ratio glucose and MDA at day 21 of

age where high PR resulted in higher plasma albumin glucose and

MDA than the corresponding values obtained of the standard PR

Meanwhile the contrary was shown in the globulin and globulin-

albumin ratio

Genotype had only a significant impact on plasma globulin-

albumin ratio where AA chicks exhibited lower value than the Hub

one There was no significant interaction between strain of broilers

thermal conditioning and CP regimen on biochemical constituents of

blood plasma mentioned before (Table 5)

Blood hematological constituents

Thermal conditioning had a significant negative effect only on

lymphocyte and esinophil (Table 6) Meanwhile the contrary was

shown in the basophil Dietary PR had no significant effect on most of

blood hematological constituents except for basophil and PI at day 21

of age Feeding high PR resulted in higher basophils and PI than

those of standard regimen The relationship between disease control

and nutritional regimen was recently reviewed (Lister 2010) In

agreement with the present results chicks fed high PR resulted in

higher persistency in antibody production at days 10 and 15 after

inoculation with sheep red blood cells than those fed the 19 CP

9


9


7


7


7


group (Praharaj et al 1998 Melo et al 1998) and supplementation

with methionine and choline significantly improved cellular and

humoral immune response (Swain and Johri 2000) Arbor acres

strain had significantly higher WBCs lymphocytes and esinophil than

Hub strain Meanwhile the contrary was shown in basophil and

heterophil

There was no significant interaction between strains of

broilers TC and PR on most of blood hematological at day 21 of age

(Table 7) expect for phagocytes activity Hub chicks exposed to C

and fed high PR had significantly higher PA than that of the same

strain exposed to TC and fed the same diet The AA chicks exposed to

TC and fed high PR had significantly higher PA than that of the same

strain kept on C and fed high and standard PR Meanwhile Hub

chicks exposed to TC and fed high PR had significantly lower PA than

that of AA chicks on the same treatments However Hub chicks

exposed to C and fed high PR had significantly higher PA than that of

AA chicks on the same treatments These findings indicated different

genetic potential between the two strains under investigation which

may be ascribed to variations in genetic makeup (Rosa et al 2007

Benyi et al 2009 Abdullah et al 2010 Yakubu et al 2010)

It could be concluded that broilers exposed to TC and fed

standard PR exhibited compensatory growth suggesting that TC and

standard PR may be suitable management tool to improve chickens

tolerance to heat stress late in life particularly in high temperature

regions

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Benyi K OAcheampong-Boateng DNorris MMathoho and

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Combination of vaccination and competitive exclusion to

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Daghir N2008 Nutrient requirements of poultry at high temperature

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Deaton JW FN Reece SL Branton and JD May 1986 High

environmental temperature and broiler livability Poult Sci

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De BasilioV MVilarino S Yahav and M Picard 2001 Early-

age thermal conditioning and a dual feeding program for male

broilers challenged by heat stress Poult Sci 8029-36

Doumas B T D Watson and H G Biggs 1977 Albumin

standards and the measurement of blood albumin with

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Eilers R J 1967 Notification of final adoption of an international

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Clin Path 47 212-214

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Kawahara E T Ueda and S Nomura 1991 In vitro phagocytic

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Aeromonas salmonicida extracelluar products Gyobyo

Kenkyu Japan 26 213-214

Koracevic D G Koracevic V Djordjevic S Andrejevic and V

Cosic 2001 Method for the measurement of antioxidant

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Lin H H C Jiao J Buyse and E Decuypere 2006 Strategies for

preventing heat stress in poultry Worlds Poult Sci J 62 71-

85

Lister S A 2010 Poultry diseases ndash their control and effects on

nutritional requirements Worlds Poult Sci J 66 633-638

Lucas A M and C Jamroz 1961 Atlas of Avian Hematology

Agriculture Monograph 25 USDA Washington DC USA

Melo J G Mallo C Campelletti E Villar M C Miquel and F

Hoffman 1998 Influence of two different protein content diets

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Medicina Veterinaria (Buenos Aires) 79169minus171

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Musharaf NA and J D Latshaw 1999 Heat increment as

affected by protein and amino acid nutrition Worldrsquos PoultSci

J 55 233-239

National Research Council (NRC) 1994 Nutrient requirement of

poultry 9th

revised Edn National Academy Press Washington

DC USA

Praharaj N K S V Ramarao M R Reddy G Shyam-Sunder

and B L N Reddy 1998 Sire by protein interactions for

growth feed efficiency and immunoresponsiveness in coloured

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Requena F V De Basilio Y Oliveros E Saume A Mendez D

Fung J Marquina and D Bastianelli 2010 Tuning

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acid Clin Chem 38 704-709

Rosa P S D E Faria Filho F Dahlke B S Vieira M Macari

and R L Fulan 2007 Effect of Energy Intake on performance

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Saleh K Y A Attia and H Younis 1996 Effect of feed restriction

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60 (4)153-159

SAS Institute (1996) SASreg

Userrsquos Guide Statistics Version 6 Edn

SAS Institute Inc Cary NC USA

Swain B K and T S Johri 2000 Effect of supplemental

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Teeter R G 1994Optimizing production of heat stressed broilers

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Teeter RG MO Smith FN Owens and SC Arp 1985

Chronic Heat Stress and Respiratory Alkalosis Occurrence and

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Temim S A M Chagneau S Guillaumein J Mitchel R

Peresson and S Tesseraud 2000 Does excess dietary Protein

improve growth performance and characteristics in heat exposed

chickens Poult Sci 79 312 ndash 317

Trinder P 1969 Determination of glucose in blood using glucose

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US National Climatic Data Center 2008 Global warming frequently

asked questions 2008

httpwwwncdcnoaagovoaclimateglobalwarminghtml

Watson D 1960 A simple method for determination of serum

cholesterol Clin Chem Acta 5 637-643

Weichselbaum TE 1946 An accurate and rapid methode for

determination of proteins in small amount of blood serum and

plasma Am J Clin Pathol 16 40ndash48

Widyaratne G P and M D Drew 2011 Effects of protein level

and digestibility on the growth and carcass characteristics of

broiler chickens Poult Sci 90595-603

Yahav S 2000 Domestic fowl-Strategies to confront environmental

conditions Avian and Poult Biol Rev 1181-95

Yahav S and JP McMurtry 2001 Thermotolerance acquisition

in broiler chickens by temperature conditioning early in life-the

effect of timing and ambient temperature Poult Sci 12 1662-

1666

Yahav S and I Plavnik 1999 Effect of early ndash age thermal

conditioning and food restriction on performance and thermo-

tolerance of male broiler chickens Br Poult Sci 40 120 ndash

126

Yakubu A J A Ayoade and Y M Dahiru 2010 Effects of

genotype and population density on growth performance

carcass characteristics and cost-benefits of broiler chickens in

north central Nigeria Trop Anim Health and Prod 42 719-

727

Yan F JH Kersey CA Fritts PW Waldroup H L Stilborn

RC Jr Crumm and DW Rice (DGKC) 1972 Empfehlungen der Deutschen Gesellschaft fuumlr Klinische

Chemie Z Klin Chimerical und Klinical Biochemsirty

10182

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Young DS CL Pestaner and U Gilberman 1975 Effects of

drugs on clinical laboratory tests Clin Chem 21 3660

الملخص العربي

للتقسة الحرارة النمو التعوض لساللتن من دجاج اللحم بعد التعرض وم 12حتى عمر ونظام البروتن الغذائ

2ولد شعبان حبش ndash 1ريسام اجا - 2وسف عبد الوهاب عطة-2ولد الطحاوي

جامعة دمنهور ndashكلة الزراعة ndash قسم االنتاج الحوان والداجن 1 اثنا-جامعة جورجا ndashقسم علوم الدواجن 2

أجرت هذه التجربة بهدف دراسة النمو التعوض لساللتن من دجاج اللحم بعد

األقتصادة وهرمون البروتن الغذائ عل الصفاتالتعرض للتقسة الحرارة ونظام تم استخدام ومكونات الدم البوكماوة والهماتولوجة الثروكسن ف بالزما الدم

كتكوت من ساللة الهابرد و نفس العدد من ساللة االربوراكرز وتم توزعهم 256تقسة حرارة ) تعرض 2نظام بروتن غذائ 2معامالت ) 8عشوائا عل ساعة عند 24 لمدة 77م ورطوبة ordm 373لكتاكت لدرجة حرارة مجموعة من ا

م ورطوبة نسبة 33أام ثم العودة ال درجة الحرارة والرطوبة الطبعة ) 3عمر وكان نظام من كل ساللة ( 45م ورطوبة نسبة ordm 33( وأخري كنترول )45

( أو قاس 24عال )وم 21عمر البروتن المتبع ف عالئق البادئ حتى(22)

أام إل انخفاض معدل 3تعرض الكتاكت ال التقسة الحرارة عند عمر أدى وم من العمر( 4-3النمو وكذلك سوء الكفاءة الغذائة خالل فترة التقسة الحرارة )

وم كان هناك تشابه ف 21-1مقارنة بمجموعة الكنترول أما خالل الفترة من عمر كما أظهرت النتائج انخفاض مستوي هرمون الثروكسن لكفاءة الغذائةمعدل النمو وا

وم للمجامع المعرضه للتقسة الحرارة مقارنة 21ف بالزما الدم عند عمر بالكنترول

4-3أدى نظام البروتن الغذائ المرتفع ال زادة معدل النمو فقط خالل فترة من ة مستوي االلبومن والجلوكوز والمالونالدهد وم من العمروكذلك زاد 21-1اام و

والكرات الدم القاعدة والدلل المناع ف بالزما الدم ولكن انخفضت النسبة مابن الجلوبولن وااللبومن ف بالزما الدم أما بالنسبة لتأثر التركب الوراث فكانت هناك

ة والفسولوجة حث تفوقت ساللة العدد من التأثرات الواضحة ف اإلستجابة اإلنتاجوم وعدد كرات الدم البضاء 21-1االربوركرز ف الصفات االنتاجة ف الفترة من

وكرات الدم الحامضة عن ساللة الهابرد الت كانت متفوقة ف النسبة مابن الجلوبولن وااللبومن وكرات الدم القاعدة والخلطة


ن الساللة والتقسة الحرارة ونظام البروتن الغذائ وقد ظهر تداخل معنوي بوم من العمر وكذلك معدل 21-1اام والفترة من 4-3عل معدل النمو خالل الفترة وم من العمر وكذلك النشاط البلعم 21-1التحول الغذائ ف الفترة من

قاس عتبران أداة مما سبق تضح أن التقسة الحرارة و نظام البروتن الغذائ المناسبة لمعاملة دجاج اللحم ف المناطق المرتفعة ف درجات الحرارة والمماثلة لظروف

تلك المنطقة الت أجرت بها


7


09

Table 3 Effect of the interaction between strain of broilers thermal conditioning and dietary protein regimen on growth performance of broiler

chicks during days 1-21 of age

SEM P value AA Hub Period

TC C TC C

Standard High Standard High Standard High Standard High

Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21

Feed intake (gbirdperiod) 2

109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21

Feed conversion ratio (feedgain) 2

0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21

1= Number of observations were 32 chicks per treatment 2 = Number of observations were 8 replicates per treatment

a b c d e Means within a row not sharing a common superscript are significantly different Pgt 005 N S= Not significant C= Control TC= Thermal condition

7


09

Table 4 Main effect of thermal conditioning dietary protein regimen and strain of broilers on blood biochemical of broiler chicks at 21d of age

Treatment TToottaall pprrootteeiinn

(gdl) AAllbbuummiinn

(gdl) GGlloobbuulliinn

(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee

PPhhoosspphhaattaassee

((IUL)

CChhoolleesstteerrooll (mgdl)

T3 (ngml)

TAC ) micromoll(

MDA( micromolL)

Thermal conditioning1

TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b

P value NS NS NS 0030 NS NS NS 00001 NS 00005

SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b

P value NS 005 005 001 0001 NS NS NS NS 002

SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144

P value NS NS NS 001 NS NS NS NS NS NS

SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052

1= Number of observations were 32 chicks per treatment

a b Means within a column not sharing a common superscript are significantly different Pgt 005 N S not significant TC=Thermal conditioning C= ControlT3=Triiodothyronine TAC= Total

antioxidant capacity MAD=malonaldeyde

7


09

Table 5 Effect of the interaction between strain of broilers thermal conditioning and dietary protein regimen on blood biochemical1 of broiler

chicks at day 99 of age

SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469

TToottaall pprrootteeiinn ggddll 0140 NS 284 296 279 289 236 299 271 269

AAllbbuummiinn gdl 0109 NS 195 196 214 200 219 203 235 200 GGlloobbuulliinn gdl 0060 NS 0701 0681 0776 0712 0933 0697 0891 0764 Globulin-Albumin ratio 0811 NS 121 112 110 117 124 125 114 129 AAllkkaalliinnee PPhhoosspphhaattaassee Ul

191 NS 193 189 191 192 192 190 189 192 CChhoolleesstteerrooll mgdl 0116 NS 246 282 294 296 253 257 285 279 T3 ngml 224 NS 531 591 512 563 531 544 514 508 TAC micromoll

0105 NS 158 153 114 154 158 165 114 141 MDA micromoll 170 NS 729 779 711 756 748 785 731 764 GGlluuccoossee microgdl


N S not significant TC=Thermal conditioning C= ControlT3=Triiodothyronine TAC= Total antioxidant capacity MAD=malonaldeyde

7


09

Table 6 Main effect of thermal conditioning dietary protein regimen and strain of broilers on blood hematology of broiler chicks at d 21 of age

PPCCVV()

HHggbb ((gL) RRBBCC`ss x10

6m

m3

PPhhaaggooccyytteess

iinnddeexx (()) PPhhaaggooccyytteess

aaccttiivviittyy (()) HHeetteerroopphhiill

(()) EEssiinnoopphhiill (())

BBaassoopphhiill

(())

MMoonnoopphhiill(()) LLyymmpphhooccyyttee

(()) WWBBCC`ss

((110033mmmm33) Treatment

Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C

NS NS NS NS NS NS 003 0006 NS 003 NS P value

0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b

167 371 210 Standard

NS NS NS 004 NS NS NS 001 NS NS NS P value

0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA

NS NS 005 NS NS 003 002 0006 NS 0001 0002 P value

0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

1= Number of observations were 32 chicks per treatment ab Means within a column not sharing a common superscript are significantly different Pgt 005 N S = not significant TC=Thermal conditioning C= Control WBC`S= white blood cell RBC`S=Red

blood cell PCV=Packed cell volume Hgb= Hemoglobin

7


09

Table 7 Effect of the interaction between strain of broilers thermal conditioning and protein regimes on blood hematology phagocytes activity

and phagocytes index1 of broiler chicks at day 21 of age

SEM P

value

Arbor Acers Hubbard Criteria

TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3

0705 NS 364 377 395 396 366 371 361 369 LLyymmpphhooccyytteess

0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil

0306 NS 787 812 881 787 756 712 800 787 EEssiinnoopphhiill

0934 NS 509 483 472 472 496 496 507 496 HHeetteerroopphhiill

0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab

PPhhaaggooccyytteess aaccttiivviittyy

(())

0049 NS 151 162 157 161 146 156 156 159 PPhhaaggooccyytteess iinnddeexx (())


a b c Means within a row not sharing a common superscript are significantly different Pgt 005 NS = not significant C=Control TC= Thermal condition WBCs= White blood cells RBCs=

Red blood cells Hgb= Hemoglobin PCV= Packed Cell Volume

7


09

8


basophils and esinophil showing AA chicks had superior

productive traits 1-21 WBCs and esinophil than Hub

chicks but had lower globulin to albumin ratio basophils

and heterophils

A significant interaction between strain TC and

protein regimen was observed on BWG during days 3-4 and

1-21 of age FCR during days 1-21 of age and phagocytes

activity (PA)

It could be concluded that broilers exposed to TC and

fed standard PR exhibited compensatory growth suggesting

that TC and standard PR may be suitable management tool

to improve chickens tolerance to heat stress late in life

particularly in high temperature regions

Keywords Thermal conditions broiler genotypes protein regimen growth

INTRODUCTION

The global mean surface temperature increased by 08 and 17 ordm

C during the 19th

and 20th

centuries and expected to increase by 06 to

25ordm C during the next 50 years

(httpwwwncdcnoaagovoaclimateglobalwarminghtml)

Increasing ambient temperatures decreases survival and productive

traits (Dale and Fuller 1979 Teeter et al 1985 Deaton et al 1986

Attia et al 2006) The negative effect of heat stress on growth

performance of broilers may be related to the sensitivity of

commercial broilers to high temperature and can be attributed in part

to the inferior development of their cardiovascular and respiratory

system in comparison to their rapid growth potential (Yahav 2000)

The high growth of broilers is connected with high feed intake and

increasing metabolic heat production (Teeter 1994)

Appropriate combination of nutritional and management

strategies are essential for optimal broiler production during heat

stress Thermal conditioning (TC) at an early age has been proposed

by various authors (Arjona et al 1990 Yahav 2000 De Basilio et

al 2001) as a possible solution to reduce susceptibility of broilers to

heat stress however the ability of broilers to recover and show

compensatory growth is addressed (Yahav 2000)

97
















21 of life


Birds and Design
















Management



97





Data Collection



































97











(1975)

























97





24 22


Soybean meal (48) 38500 3300



Limestone 1300 135

NaCl 0400 040

Premix1 0300 030




Sand 0000 000

Total 100 100


3 chemical analysis

ME kcalkg 3098 3099

DM 8998 8683

CP 2 2408 2201

CP 3

2421 2266

Lysine 2

126 125


SSA 2 093 093

Threonine 2 102 094

Arginine 2 169 154

Tryptophan 2

033 029

Calcium 2 101 102


Ash 3 682 684






97





Thermal condition1

521 788b

TC

524 132a

C

NS 00001 P value

322 0315 SEM

Protein regimen1

527a

110a

High

517b

101b

Standard

003 0046 P value

322 0315 SEM

Strain1

507 b

112 a

Hub

537 a

984 b

AA

00001 0006 P value

322 0315 SEM


Thermal condition2

664b

190b

TC

690a

216a

C

0004 00011 P value

629 0544 SEM

Protein regimen2

681 200 High

673 206 Standard

NS NS P value

629 0544 SEM

Strain2

671 208 Hub

683 198 AA

NS NS P value

629 0544 SEM


Thermal condition2

128 235a

TC

131 159b

C

NS 00001 P value

97



0013 0072 SEM

Protein regimen2

129 187 High

130 207 Standard

NS NS P value

0013 0072 SEM

Strain2

131 199 Hub

128 195 AA

NS NS P value

























98


99





















albumin ratio

















9


9


7


7


7







heterophil



















regions

REFERENCES











7





J 21 521-547















203261-3282

















7







651268-1269






















85









7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

97
















21 of life


Birds and Design
















Management



97





Data Collection



































97











(1975)

























97





24 22


Soybean meal (48) 38500 3300



Limestone 1300 135

NaCl 0400 040

Premix1 0300 030




Sand 0000 000

Total 100 100


3 chemical analysis

ME kcalkg 3098 3099

DM 8998 8683

CP 2 2408 2201

CP 3

2421 2266

Lysine 2

126 125


SSA 2 093 093

Threonine 2 102 094

Arginine 2 169 154

Tryptophan 2

033 029

Calcium 2 101 102


Ash 3 682 684






97





Thermal condition1

521 788b

TC

524 132a

C

NS 00001 P value

322 0315 SEM

Protein regimen1

527a

110a

High

517b

101b

Standard

003 0046 P value

322 0315 SEM

Strain1

507 b

112 a

Hub

537 a

984 b

AA

00001 0006 P value

322 0315 SEM


Thermal condition2

664b

190b

TC

690a

216a

C

0004 00011 P value

629 0544 SEM

Protein regimen2

681 200 High

673 206 Standard

NS NS P value

629 0544 SEM

Strain2

671 208 Hub

683 198 AA

NS NS P value

629 0544 SEM


Thermal condition2

128 235a

TC

131 159b

C

NS 00001 P value

97



0013 0072 SEM

Protein regimen2

129 187 High

130 207 Standard

NS NS P value

0013 0072 SEM

Strain2

131 199 Hub

128 195 AA

NS NS P value

























98


99





















albumin ratio

















9


9


7


7


7







heterophil



















regions

REFERENCES











7





J 21 521-547















203261-3282

















7







651268-1269






















85









7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

97





Data Collection



































97











(1975)

























97





24 22


Soybean meal (48) 38500 3300



Limestone 1300 135

NaCl 0400 040

Premix1 0300 030




Sand 0000 000

Total 100 100


3 chemical analysis

ME kcalkg 3098 3099

DM 8998 8683

CP 2 2408 2201

CP 3

2421 2266

Lysine 2

126 125


SSA 2 093 093

Threonine 2 102 094

Arginine 2 169 154

Tryptophan 2

033 029

Calcium 2 101 102


Ash 3 682 684






97





Thermal condition1

521 788b

TC

524 132a

C

NS 00001 P value

322 0315 SEM

Protein regimen1

527a

110a

High

517b

101b

Standard

003 0046 P value

322 0315 SEM

Strain1

507 b

112 a

Hub

537 a

984 b

AA

00001 0006 P value

322 0315 SEM


Thermal condition2

664b

190b

TC

690a

216a

C

0004 00011 P value

629 0544 SEM

Protein regimen2

681 200 High

673 206 Standard

NS NS P value

629 0544 SEM

Strain2

671 208 Hub

683 198 AA

NS NS P value

629 0544 SEM


Thermal condition2

128 235a

TC

131 159b

C

NS 00001 P value

97



0013 0072 SEM

Protein regimen2

129 187 High

130 207 Standard

NS NS P value

0013 0072 SEM

Strain2

131 199 Hub

128 195 AA

NS NS P value

























98


99





















albumin ratio

















9


9


7


7


7







heterophil



















regions

REFERENCES











7





J 21 521-547















203261-3282

















7







651268-1269






















85









7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

97











(1975)

























97





24 22


Soybean meal (48) 38500 3300



Limestone 1300 135

NaCl 0400 040

Premix1 0300 030




Sand 0000 000

Total 100 100


3 chemical analysis

ME kcalkg 3098 3099

DM 8998 8683

CP 2 2408 2201

CP 3

2421 2266

Lysine 2

126 125


SSA 2 093 093

Threonine 2 102 094

Arginine 2 169 154

Tryptophan 2

033 029

Calcium 2 101 102


Ash 3 682 684






97





Thermal condition1

521 788b

TC

524 132a

C

NS 00001 P value

322 0315 SEM

Protein regimen1

527a

110a

High

517b

101b

Standard

003 0046 P value

322 0315 SEM

Strain1

507 b

112 a

Hub

537 a

984 b

AA

00001 0006 P value

322 0315 SEM


Thermal condition2

664b

190b

TC

690a

216a

C

0004 00011 P value

629 0544 SEM

Protein regimen2

681 200 High

673 206 Standard

NS NS P value

629 0544 SEM

Strain2

671 208 Hub

683 198 AA

NS NS P value

629 0544 SEM


Thermal condition2

128 235a

TC

131 159b

C

NS 00001 P value

97



0013 0072 SEM

Protein regimen2

129 187 High

130 207 Standard

NS NS P value

0013 0072 SEM

Strain2

131 199 Hub

128 195 AA

NS NS P value

























98


99





















albumin ratio

















9


9


7


7


7







heterophil



















regions

REFERENCES











7





J 21 521-547















203261-3282

















7







651268-1269






















85









7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

97





24 22


Soybean meal (48) 38500 3300



Limestone 1300 135

NaCl 0400 040

Premix1 0300 030




Sand 0000 000

Total 100 100


3 chemical analysis

ME kcalkg 3098 3099

DM 8998 8683

CP 2 2408 2201

CP 3

2421 2266

Lysine 2

126 125


SSA 2 093 093

Threonine 2 102 094

Arginine 2 169 154

Tryptophan 2

033 029

Calcium 2 101 102


Ash 3 682 684






97





Thermal condition1

521 788b

TC

524 132a

C

NS 00001 P value

322 0315 SEM

Protein regimen1

527a

110a

High

517b

101b

Standard

003 0046 P value

322 0315 SEM

Strain1

507 b

112 a

Hub

537 a

984 b

AA

00001 0006 P value

322 0315 SEM


Thermal condition2

664b

190b

TC

690a

216a

C

0004 00011 P value

629 0544 SEM

Protein regimen2

681 200 High

673 206 Standard

NS NS P value

629 0544 SEM

Strain2

671 208 Hub

683 198 AA

NS NS P value

629 0544 SEM


Thermal condition2

128 235a

TC

131 159b

C

NS 00001 P value

97



0013 0072 SEM

Protein regimen2

129 187 High

130 207 Standard

NS NS P value

0013 0072 SEM

Strain2

131 199 Hub

128 195 AA

NS NS P value

























98


99





















albumin ratio

















9


9


7


7


7







heterophil



















regions

REFERENCES











7





J 21 521-547















203261-3282

















7







651268-1269






















85









7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

97





Thermal condition1

521 788b

TC

524 132a

C

NS 00001 P value

322 0315 SEM

Protein regimen1

527a

110a

High

517b

101b

Standard

003 0046 P value

322 0315 SEM

Strain1

507 b

112 a

Hub

537 a

984 b

AA

00001 0006 P value

322 0315 SEM


Thermal condition2

664b

190b

TC

690a

216a

C

0004 00011 P value

629 0544 SEM

Protein regimen2

681 200 High

673 206 Standard

NS NS P value

629 0544 SEM

Strain2

671 208 Hub

683 198 AA

NS NS P value

629 0544 SEM


Thermal condition2

128 235a

TC

131 159b

C

NS 00001 P value

97



0013 0072 SEM

Protein regimen2

129 187 High

130 207 Standard

NS NS P value

0013 0072 SEM

Strain2

131 199 Hub

128 195 AA

NS NS P value

























98


99





















albumin ratio

















9


9


7


7


7







heterophil



















regions

REFERENCES











7





J 21 521-547















203261-3282

















7







651268-1269






















85









7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

97



0013 0072 SEM

Protein regimen2

129 187 High

130 207 Standard

NS NS P value

0013 0072 SEM

Strain2

131 199 Hub

128 195 AA

NS NS P value

























98


99





















albumin ratio

















9


9


7


7


7







heterophil



















regions

REFERENCES











7





J 21 521-547















203261-3282

















7







651268-1269






















85









7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

98


99





















albumin ratio

















9


9


7


7


7







heterophil



















regions

REFERENCES











7





J 21 521-547















203261-3282

















7







651268-1269






















85









7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

99





















albumin ratio

















9


9


7


7


7







heterophil



















regions

REFERENCES











7





J 21 521-547















203261-3282

















7







651268-1269






















85









7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

9


9


7


7


7







heterophil



















regions

REFERENCES











7





J 21 521-547















203261-3282

















7







651268-1269






















85









7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

9


7


7


7







heterophil



















regions

REFERENCES











7





J 21 521-547















203261-3282

















7







651268-1269






















85









7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

7


7


7







heterophil



















regions

REFERENCES











7





J 21 521-547















203261-3282

















7







651268-1269






















85









7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

7


7







heterophil



















regions

REFERENCES











7





J 21 521-547















203261-3282

















7







651268-1269






















85









7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

7







heterophil



















regions

REFERENCES











7





J 21 521-547















203261-3282

















7







651268-1269






















85









7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

7





J 21 521-547















203261-3282

















7







651268-1269






















85









7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

7







651268-1269






















85









7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

7




J 55 233-239


poultry 9th


DC USA





















60 (4)153-159












8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

8








Biochem 6 24-26

















1666




126





727




10182

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

9

























7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09






7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09


7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

7


09




TC C TC C


Body weight gain1

0630 004 768d

517e

125ab

141a

835d

104c

118bc

142a

Days 3-4

200 00003 546b

519c

517c

568a

509cd

512cd

497d

511cd

Day 1-21


109 NS 179 171 238 205 190 219 216 206 Days 3-4

126 NS 664 661 707 702 659 671 663 690 Day 1-21


0143 NS 218 251 184 125 230 242 195 132 Days 3-4

0025 003 125e

127de

137 a

125e

128dce

131bc

129 dc

133b

Day 1-21



7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

7


09





(gdl)

Globulin

albumin

ratio

GGlluuccoossee

(microgdl)

AAllkkaalliinnee


((IUL)


T3 (ngml)

TAC ) micromoll(

MDA( micromolL)


TC 482 279 203 0753b 760 121 191 245b 549 158a

C 489 277 212 0782 a 741 117 191 280a 524 131b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Protein regimen1

High 488 288a 200b 0713b 771a 121 191 268 551 153a

Standard 483 268b 215a 0822a 730b 117 191 257 522 134b


SEM 0076 0070 0055 0031 0850 0405 0955 0058 112 0052

Strain1

HUB 483 269 214 0821a 757 123 191 261 524 144

AA 488 287 201 0714b 744 115 191 264 549 144


SEM 0076 0070 0055 0030 0850 0405 0955 0058 112 0052




7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

7


09



SEM P

value

AA Hub

Criteria TC C TC C


0153 NS 479 493 492 489 455 501 506 469







7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

7


09


PPCCVV()


6m

m3





BBaassoopphhiill

(())


(()) WWBBCC`ss


Thermal condition1

273 886 171 153 178 496 767b 425a 155 369b 210 TC

279 909 168 158 176 487 814a 352b 166 380a 207 C


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Protein regimen1

275 891 170 159a

179 487 775 423a

153 378 207 High

278 905 170 152b

175 496 806 353b



0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM

Strain1

274 883 167 154 177 499a 764b 425a 158 383a 204b Hub

279 912 173 158 177 484b 817a 352b 163 367b 213a AA


0202 0171 0023 0024 0226 0467 0153 0190 0090 0352 0223 SEM



7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

7


09



SEM P

value


TC C TC C


0223 NS 217 218 211 208 203 204 211 197 WWBBCC`ss x103mm

3


0180 NS 163 150 175 163 163 144 167 156 MMoonnophil

0380 NS 325 437 275 369 462 475 350 412 BBaassophil



0045 NS 178 171 168 174 167 168 166 166 RRBBCC`ss x106mm

3

0343 NS 900 881 950 919 867 894 900 869 HHggbb gdL

0900 NS 277

271

286

283

271

274

277

272

PPCCVV

0452 002 178abc

189a

173c

169c

173c

171c

174bc

187ab


(())





7


09

7


09

Documents

COMPENSATORY GROWTH OF TWO BROILER … · COMPENSATORY GROWTH OF TWO BROILER GENOTYPES FOLLOWING THERMAL CONDITIONING AND CRUDE ... 21 days according to the ... during days 3-4 of