COMPARATIVE STUDY OF SWETA PARPATI & SHEETAL PARPATI IN MUTRAKRICHRA W.S.R

TO THEIR ANTI MICROBIAL STUDY - BY DR. NITU SINGH P.G SCHOLAR DEPARTMENT OF RASA SHASTRA

Dr P G Jadar. MD

Dept of Rasashastra

K.L.E’s Shri B M K Ayurveda Mahavidyalaya. Belgaum, Karnataka.

Dr R S Hiremath. MD

Dept of Rasashastra

NEED FOR STUDY

• There are many formulations told for mutrakrichra in Ayurveda. Shweta parpati and sheetal parpati are two such preparations

• It is usually prepared from kajjali or by adding some ingredients to kajjali.

• Sweta parpati is nirgandha, and sheetal parpati is sagandha preparation .

OBJECTIVES• Physical and chemical analysis of raw

materials• Shodhana of ingredients and preparation of

Shweta parpati and sheetal parpati. • Analytical study of prepared compounds&

Comparative evaluation of antimicrobial activity of both formulations on -

1) E.coli 2) Proteus mirabilis 3) Staphylococcus aureus 4) Candida albicans

Raw materials were purchased and authentificated.

Kalmisora nirmalikarana.

Preparation of ela hima.

Kalmisora shodhana -- in order to enhance its diuretic property.

MATERIALS & METHODS

Kankshi shodhana.

Navsadara shodhana.

Preparation of triphala kwatha.

Gandhaka shodhana.

Preparation of shweta parpati:

Kalmi sora-96 gm

shodhita kankshi-12 gm

Shodhita navasadar-6gm

Preparation of sheetal parpati:

Kalmi sora-128gm

Shodhita gandhaka-2 gm

ANALYTICAL PROCEDURES

QUALITATIVE ANALYSIS

Basic radicals : Na+, K+, NH4+, Al+3, Zn+2, Ba+2, Ca+2 ,

Mg+2

Acid radicals: Cl- , Br-, So4, No3 may be present in the compounds.

Determination of pH values-

It is noted about 4.19 in shweta parpati & 6.76 in sheetal parpati. by help of ph meter

Result

Material Before Shodhana After Shodhana

SORA Pandura Sikatakara

Odourless Karpurabha

Pandura Small crystals

Smell of Ela Opaque

KANKSHI Yellowish White; Transparent

Hard Crystals ; Guru,Snigdha

Clear White ; Amorphous

Soft powder ; Laghu, Ruksha

NAVASADAR White

Crystalline

Odourless

White

Crystalline

Odourless

GANDHAKA Greenish yellow

Snigdha

Hard crystals

Yellow

Snigdha

Brittle Crystals

Organoleptic Characteristics:

Contd…

Shweta parpati:

• Colour: white

• Odour: Kshariya

• Touch: smooth

• Taste: lavana,sheeta

• pH: 4.19

• Total ash: 132.8451%

• Total chloride: 3.1475%

• Total sulphate: 4.0824%

• Qualitative test for K+&Al3+: Positive

Sheetal parpati:

• Colour: light brown

• Odour: smell of gandhaka

• Touch: rough

• Taste: lavana,sheeta

• pH: 6.76

• Total ash: 99.1011%

• Total sulphates: 0.2141%

• Qualitative test for K+ & Fe+3: Positive

Antimicrobial Activity

% conc

E. coli S. aureus Pr. mirabilis C. albicans

A B A B A B A B

1%

2.5%

5%

7.5%

10%

+VE

+VE +VE

+VE +VE+VE

+VE

+VE

+VE

+VE

+VE

+VE

+VE

+VE

-VE

-VE-VE

-VE

-VE

-VE-VE

-VE -VE

-VE

-VE

-VE

-VE

-VE

-VE

-VE

-VE

-VE

-VE-VE

-VE

-VE

-VE -VE

-VE

-VE

Colony morphology for identification of micro organisms

1. S.aureus form large colonies of 2-4 mm dia,yellowish Circular clusters

2. E.coli form large ,thick grayish colonies.

3. P.mirabilis show swarming motility& negative to lactose.4. C.albicans are yeast like fungus .

5. Agar diffusion method-None of dilution show zone of inhi bition for any organisms.

6. Agar dilution method-A: Shweta parpati ,B: Sheetal parpati Growth indicated by +VE No growth indicated by -VE

CONCLUSION

1.The pH of both parpatis fall in range that is required for bacterial growth. So it can be said that action of parpatis are independent of change in pH.

2.The two parpatis show encouraging antimicrobial activities at different concentrations in different strains of bacteria.

3. Antibiotic susceptibility tests are not guarantee that an Anti microbial activity will be effective or ineffective

4.The general method of diagnosis in ayurveda is based on doshas like vata ,pitta and kapha.So ayurvedic formulations have different role in every human being.

5.This study gives a scope for in vivo studies regarding action of these formulations in reducing puss and albumin.

THANK U