Cold Spring Harbor Laboratory Press

Fly Pushing The Theory and Practice of Drosophila Genetics By Ralph J. Greenspan, N e w York University

Experiments with Drosophila give investigators the chance to combine molecular and genetic ap- proaches in studies of gene expression, cell function, and development. Fly genetics has a large and com- plex folklore that, for the uninitiated, is quite daunt- ing. In this eagerly awaited book, Ralph Greenspan provides a bridge to that folklore. Entertaining and lucid, he guides the reader carefully through the prac- ticalities of making crosses, isolating variants, map- ping genes, constructing specific genotypes, and an- alyzing mutations. No previous knowledge of fly ge- netics is assumed. The techniques used are illus- trated, and practice problems and solutions are in- eluded to assist the reader.

This is an affordable and uniquely valuable book, ideal for graduate students starting projects and estab- lished investigators considering new avenues. A must for both personal and laboratory libraries.

C O N T E N T S

Introduction Chapter One: The Basics of Doing a Cross Fly Chromosomes; Recognizing Markers; Nomenclature; More about Balancers; Deciphering Mating Schemes; Basic Fly Hus- bandry; Coddling Difficult Strains; Collecting Flies for Crosses Chapter Two: Isolating New Variants Principles; Mutagens: Ethylmethane sulfonate (EMS), Ethyl- nitrosourea (ENU), Radiation, Insertional mutagenesis; Genetic Schemes for Detecting Mutants: New alleles at an autosomal locus, New alleles on the X, Identifying new genes by mutagenesis, Isogenize the starter stock, The mutagenesis screen, Screening the X for new genes, Insertional mutagenesis and enhancer trapping, Enhancer-trap screens, New insertional alleles of known loci, Mutating genes for which there are no allelic differences, Screen- ing over a deletion of the locus, lnsertional mutations of cloned genes, Biochemical assays for mutations Chapter Three: Mapping Segregation analysis, Problems with balancers, Meiotic mapping, Deletion mapping, Duplication mapping, Synthesis of deletions and duplications, Identification of new embryonic genes by dele- tion mapping, Mapping by in situ hybridization Chapter Four: Synthesizing Specific Genotypes Principles, Simple manipulations of a single chromosome, Linking mutations, Manipulating two chromosomes, Pitfalls: How Many Flies?, Which Sex?, Manipulating three chromosomes, Making rearrangements, Induction of deletions, Synthesis of stable dele-

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P U B L I S H E D !

tions from T(Y;A)s, Synthesizing deletions from P-element inser- tions, Inducing duplications, Synthesis of stable duplications from T(Y;A)s, Synthesizing attached-X's, Autosynaptic chromosomes and the joys of gibberish Chapter Five: Analysis of Mutations: I. Characteristics of the Allele Formal Classification of Mutations: Principles, Null alleles (amorphs), Hypomorphs, Hypermorphs, Neomorphs, Antimorphs, Gain vs. loss of function, Intragenic interactions; Interacting Loci: Ordering genes in a pathway, Dosage-dependent interactions; Con- ditional Alleles: Inducible promoters; Maintenance of Mutant Phenotypes Chapter Six: Analysis of Mutations: II. Mosaics Principles; Chromosome Loss: Uses of chromosome loss mosaics; Fate Mapping; Focus of Gene Action: Methods for inducing chromosome loss, Ring loss, Chromosome loss mosaics for autosomal genes, Paternal-loss (pal), Claret-non-disjunctionai (cand), Care of mosaic-producing stocks; Mitotic Recombination: Synchronizing age of cultures, Uses of mitotic recombination mosaics; Lineage Analysis; Focus of Gene Action; Time of Gene Action: Germ-line clones, Methods for producing mitotic recom- binant clones; Radiation; The FLP-FRT System: Markers for inter- nal tissues; GAL4 and Expression Mosaicism: Cell ablations Chapter Seven: You're on Your Own References

1997, 155 pp., illus., appendices, index Cloth $35 ISBN 0-87969-492-0

&Development VOLUME 11 NUMBER 12 PAGES 1493-1620 June 15, 1997

E D I T O R I A L B O A R D

J. Adams (Melbourne, Australia) J. Beckwith (Boston, USA) A. Berns (Amsterdam, The Netherlands) E. Blackburn (San Francisco, USA) D. Bohmann (Heidelberg, Germany) J. Brugge (Cambridge, USA) T. Cech (Boulder, USA) P. Chambon (Strasbourg, France) N.-H. Chua (New York, USA) S. Courtneidge (Redwood City, CA USA) S. Elledge (Houston, USA) R. Evans (La Jolla, USA) G. Fink (Cambridge, USA) P. Goodfellow (Harlow, UK) S. Gottesman (Bethesda, USA) C. Gross (San Francisco, USA) R. Grosschedl (San Francisco, USA) M. Groudine (Seattle, USA) L. Guarente (Cambridge, USA) E. Hafen (Zurich, Switzerland) S. Hake (Albany, CA USA) A. Hall (London, UK) R. Harland (Berkeley, USA) E. Harlow (Charlestown, USA) W. Herr (Cold Spring Harbor, USA) J. Hodgkin (Cambridge, UK) J.H.J. Hoeijmakers (Rotterdam, The

Netherlands) R. Horvitz (Cambridge, USA) P. Ingham (Sheffield, UK)

Y.-N. Jan (San Francisco, USA) T. Jessell (New York, USA) N. Jones (London, UK) J. Kadonaga (La Jolla, USA) R. Lehmann (New York, USA) M. Levine (Berkeley, USA) D. Livingston (Boston, USA) J. Manley (New York, USA) R. Martienssen (Cold Spring Harbor, USA) M. Mathews (Newark, NJ USA) W. McGinnis (La Jolla, USA) R. McKay (Bethesda, USA) S. McKnight (Dallas, USA) A. McMahon (Cambridge, USA) L. Parada (Dallas, USA) C. Prives (New York, USA) U. Schibler (Geneva, Switzerland) J. Schlessinger (New York, USA) P. Sharp (Cambridge, USA) C. Sherr (Memphis, USA) D. Solter (Freiburg, FRG) P. Soriano (Seattle, USA) J. Steitz (New Haven, USA) P. Stragier (Paris, France) M. Takeichi (Kyoto, Japan) T. Taniguchi (Tokyo, Japan) S. Tilghman (Princeton, USA) R. Tjian (Berkeley, USA) M. Wigler (Cold Spring Harbor, USA)

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Contents GENES & DEVELOPMENT June 15, 1997

Research papers An activating mutation in a Caenorhabditis elegans Gs protein induces neural degeneration Hendrik C. Korswagen, Jong-Hyuk Park, Yasumi Ohshima, and Ronald H.A. Plasterk

1493

ORC-dependent and origin-specific initiation of DNA replication at defined loci in isolated yeast nuclei Philippe Pasero, Diego Braguglia, and Susan M. Gasser

1504

The Spglp GTPase is an essential, dosage-dependent inducer of septum formation in Schizosaccharomyces pombe Susanne Schmidt, Marc Sohrmann, Kay Hofmann, Alison Woollard, and Viesturs Simanis

1519

Nedd5, a mammalian septin, is a novel cytoskeletal component interacting with actin-based structures Makoto Kinoshita, Sharad Kumar, Akira Mizoguchi, Chizuka Ide, Ayae Kinoshita, Tokuko Haraguchi, Yasushi Hiraoka, and Makoto Noda

1535

Fission yeast WD-repeat protein Popl regulates genome ploidy through ubiquitin- proteasome-mediated degradation of the CDK inhibitor Ruml and the S-phase initiator Cdcl8 Kin-ichiro Kominami and Takashi Toda

1548

CIpX and MuB interact with overlapping regions of Mu transposase: implications for control of the transposition pathway Igor Levchenko, Michael Yamauchi, and Tania A. Baker

1561

Mlhl is unique among mismatch repair proteins in its ability to promote crossing.over during meiosis Neil Hunter and Rhona H. Borts

1573

The mesenchymal winged helix transcription factor Fkh6 is required for the control of gastrointestinal proliferation and differentiation Klaus H. Kaestner, Debra G. Silberg, Peter G. Traber, and G~inther Sch6tz

1583

(continued)

Deletion of the H19 transcription unit reveals the existence of a putative imprinting control element Mar ie -Anne Ripoche, Cha n t a l Kress, Fran~oise Poirier, and Luisa Dandolo

1596

SV40 large T antigen binds to the TBP-TAF~ complex SL1 and coactivates ribosomal RNA transcription Weiguo Zhai, JoAnn Tuan , and Lucio C o m a i

1605

Erratum 1618

Product News 1619

Cover DNA replication at defined foci in isolated yeast nuclei. (Background) Wild-type yeast nuclei incubated in biotin-dUTP nuclear extract. (Inset, top left) Labeled S-phase nucleus. (Inset, bottom right) Surface topography of labeled S-phase nucleus. (For details, see Pasero et al., p. 1504.)