5
DOI 10.13350 j.cjpb.191108 ·!"· cmnopG 01883 * cd+P /wEu  k>? ô@¬ ÍðA mjñ BCD kE 1. *&+&234u 750004 2. *+%&+43. *+%&@½3*+%&01*&,!"#$ L6ËõÆq;g_^` ; = Q GH  è;n Eg01883 YQK« ;< = {AòK ;<={ÈÉKL ¤×Ñ|}%& Z6Æ SOPMA ¤ DNAStarq Eg01883 ÁL M56 SWISSModelYQhDEÁLML6 Æ ABCPredNIEDB LâKo `  ´µ  = e ¤  è; S ÁLMNÁLMvK ;<={wxqYQâ={Â?U9.DErEg01883ÈÉB.èà*J¨è56q¿ ELISA [\FGÈÉKL _={$yÙUÃÄ;g_^`B*J¨èELISA 2QKÈÉKL Õ|K×Ñ|}'( 0©FG è; O à Eg01883= {;< = { ¾ j{§1-15 19-34 155-169 180-196 216-232 236-248 271-287{0â¡ + = {BJ 99.99% Z69.DEÈÉà*FGKL_Yk={19-34N180-196 ;g_^ÃÄB*«WÂZ ;<={450 }ÂJ0.8218±0.0406N 0.6398±0.0447 -v 2 a 450 } 0.3312±0.0267 N 0.2961± 0.0284 ZíHÁøË&ùú0.05 )。 ') FG F n ;< = { × Ñ è; Âvw¨© ó _ ;g_^`ÿ×Ñè;N23Yk={ÉÀ÷øf¸ùú*+,;g_^`×Ñè;Â;<={rEg01883 .VWX& -./01R383.33 23456278116735234 2019 11127705 犑狅狌狉狀犪犾狅 犘犪狋犺狅 犲狀犅犻狅犾狅 犵狔.2019Nov 14 11 ): 1277-1281. 犘狉犲犱犻犮狋犻狅狀 狊犮狉犲犲狀犻狀犵 犪狀犱犱犲狋犲狉犿犻狀犪狋犻狅狀狅犳狋犺犲狊犲狉狅犾狅犵犻犮犪犾犱犻犪犵狀狅狊狋犻犮狏犪犾狌犲狅犳犅犮犲犾犾犲狆犻狋狅狆犲狊狅犳 犈犮犺犻狀狅 犮狅犮犮狌狊 狉犪狀狌犾狅狊狌狊犈犵01883 ZHAOYinqi XUShimei ZHU Mingxing ZHANGTingrui LV Yongxue ZHAORui ZHAOWei 犖犻狀 狓犻犪犐狀狊狋犻狋狌狋犲狅 犕犲犱犻犮犪犾犛犮犻犲狀犮犲 犢犻狀犮犺狌犪狀 犖犻狀 狓犻犪 犆犺犻狀犪750004 犛犮犻犲狀犮犲犪狀犱犜犲犮犺狀狅犾狅 犵狔 犆犲狀狋犲狉 犖犻狀 狓犻犪犕犲犱犻犮犪犾犝狀犻狏犲狉狊犻狋 犈犮犺犻狀狅犮狅犮犮狅狊犻狊犔犪犫狅狉犪狋狅狉 犖犻狀 狓犻犪犕犲犱犻犮犪犾犝狀犻狏犲狉 狊犻狋 犛犮犺狅狅犾狅 犅犪狊犻犮犕犲犱犻犮犻狀犲 犖犻狀 狓犻犪犕犲犱犻犮犪犾犝狀犻狏犲狉狊犻狋 犃犫狊狋狉犪犮狋 犗犫犼犲犮狋犻狏犲 Tousecomputersoftwaretoanalyze Eg01883 aspecificantigen moleculeof犈犮犺犻狀狅犮狅犮犮狌狊 狉犪狀狌犾狅狊狌狊 inordertopredictthepossibleformationofBcellepitopesandtoexploretheimmunoreactivityanddiagnostic valueofBcellepitopes. 犕犲狋犺狅犱狊 ThesecondarystructureofEg01883wasanalyzedusingtheonlinesoftwareSOPMA andDNAStar andthetertiarystructureoftheproteinwaspredictedusingSWISSModel.TheonlinesoftwareABCPred andIEDB wereusedtoanalyzetheprotein'ssecondaryandtertiarystructureandcharacteristicssuchashydrophilicity flexibility surfaceaccessibility antigenicity Bcellepitopes andartificialsynthesisofepitopepeptides.Antiserumwas collectedfrommiceimmunizedwithrEg01883 andtheimmunoreactiveepitopewasscreenedusingELISA.Serumfrom miceinfectedwith狉犪狀狌犾狅狊狌狊wasusedastheprimaryantibodytoevaluateitsimmunoreactivityanddiagnosticvalue withELISA. 犚犲狊狌犾狋狊 SevenEg01883epitopeswithahighantigenicindexwereinitiallyscreened.TheBcellepitopes werelocatedatpositions1-15 19-34 155-169 180-196 216-232 236-248 and271-287.Theseepitopes weresynthesizedwithapurityof99.99%.Twosuperiorantigenicepitopes 19-34and180-196 werescreenedusing Eg01883immunizedserum.ThesetwoBcellepitopes wererecognizedbytheseruminfected with狉犪狀狌犾狅狊狌狊 .In comparisontothecontrol theA450valuesintheepitopegroupswere0.8218±0.0406and0.6398±0.0447 those valuesdifferedsignificantlyfromthevaluesinthecontrolgroups 0.3312±0.0267and0.2961±0.0284 )( 0.05 · · 4 56789:;< 犑狅狌狉狀犪犾狅 犘犪狋犺狅 犲狀犅犻狅犾狅 犵狔 2019û11ü ý14þý11ÿ Nov.2019 Vol.14 No.11 9:;##$ +&0 %&' No.NZ17207 ); *+%& ¿Á §¨ No.XY2017013 ); !"#$ +&0 %&' No. 81360249 )。 <=>? Email zw6915 163.com >?@A¡Ý1989- ), -Â/023.§/35ϸ]nnÃÄ-ÈÉ&23Email 460647309 qq.com

cmnopG—ŁŒŠ— Eg 01883 cd+ŸŽP ıł/wEœušž · 2020. 6. 15. · DOI:10.13350/j.cjpb.191108 ·!"· cmnopG—ŁŒŠ—Eg01883*Bcd+ŸŽP、 ıł/wEœušž € k>?1,

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  • DOI:10.13350/j.cjpb.191108 ·!"·

    cmnopGEg01883*Bcd+P、

    /wEu 

    k>?

    1,2,3,ô@¬

    1,2,3,ÍðA

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    2,4,BCD

    2,4,kñ

    2,kE

    1,2,3

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    【!"

    】 #$ L6ËõÆq;g_^`;=QGH è;nEg01883,YQK«B;

  •  Conclusion TwopotentialBcellepitopepeptidesweresuccessfullyscreened.Thisfindingiscrucialforidentification

    ofearlydiagnosticantigensof犈.犵狉犪狀狌犾狅狊狌狊anddevelopmentofepitopebasedvaccines.

    【犓犲狔狑狅狉犱狊】 犈犮犺犻狀狅犮狅犮犮狌狊犵狉犪狀狌犾狅狊狌狊;diagnosticantigenpeptide;Bcellepitopes;recombinantprotein犈犵01883;

    bioinformatics

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    .3 犈犵01883$DS、E¹S、äØF¬、YGHSÁ{

    犉犻犵.3 犉犾犲狓犻犫犾犲,犺狔犱狉狅狆犺犻犾犻犮犻狋狔,犪狀狋犻犵犲狀犻犮犻狀犱犻狓犪狀犱狊狌狉犳犪犮犲狆狉狅犫犪犫犾犻狋狔狆狉犲犱犻犮狋犲犱狅犳犪狀狋犻犵犲狀犈犵01883

       .4 犈犵01883$ÄÃ'ÀÁ{

    犉犻犵.4 犜犲狉狋犻犪狉狔狊狋狉狌犮狋狌狉犲狆狉犲犱犻犮狋犻狅狀狅犳犪狋犻犵犲狀犈犵01883

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    犑狅狌狉狀犪犾狅犳犘犪狋犺狅犵犲狀犅犻狅犾狅犵狔 2019û11ü ý14þý11ÿ

    Nov.2019, Vol.14,No.11

  • 5 犈犵01883$犅¤¥YÙ

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