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LABORATORY MANUAL

Exercise/Experiment Number:

Lab Code / Lab : BTY412 Bioinformatics Lab

Course / Branch : IV B.Tech. BiotechnologyTite o! the exercise/experiment : Multiple sequence alignment !L"#T$L %& an'Molecular phylogeny ______________________________________________________________ 

 "TE# $: %NTRO&UCT%ON

OB'ECT%(E O) T*E E+ERC%"E/E+#ER%MENT• To un'erstan' an' perform the multiple sequence alignment an'

 phylogenetic analysis using !L"#T$L % an' ()YLI(* respecti+ely.

"TE# ,: AC-U%"%T%ONINTRODUCTION

$ multiple sequence alignment M#$& is a sequence alignment of three or more

 biological sequences protein* ,-$* or -$&. /rom the query sequences 0e can fin' the

e+olutionary relationship by 0hich they share a lineage an' the 'escen'e' from a common

ancestor. Because three or more sequences of biologically rele+ant length can be 'ifficult

an' are almost al0ays timeconsuming to align by han'* computational algorithms are

use' to pro'uce an' analye the alignments. M#$s require more sophisticate'

metho'ologies than pair0ise alignment because they are more computationally comple3.

#rocedure:

#tep 1 Login to 5MBL6 5BI 0ebsite an' select !L"#T$L 7mega option un'er 

sequence analysis

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#tep 2 #elect the type of the molecule to be clustere'

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#tep 8 #elect an' appen' the sequences in the appropriate bo3

#tep 4 un the 'efault parameters an' submit. (aste the screenshot of the

alignment pro'uce'

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esult

The !lustal program 0as use' to align relate' sequences